Image Guided Automated Non-Prehensile Magnetic Micromanipulation of Cells

2015 ◽  
Author(s):  
Akash Das ◽  
Ajay D. Thakur ◽  
Atul Thakur
Keyword(s):  
Target Cell ◽  
Targeted Drug Delivery ◽  
Biomedical Applications ◽  
Cell Manipulation ◽  
Target Cells ◽  
Image Guided ◽  
Targeted Drug ◽  
And Control ◽  
Minimal Adverse Effect ◽  
Ferromagnetic Particles

Biomedical applications like cell manipulation and targeted drug delivery require automated micro-manipulation of biological material. Magnetic micro-manipulation has high actuation speed and minimal adverse effect on cell viability. Ferromagnetic particles, actuated via magnetic field, are used to push a target cell. The process is however cumbersome therefore require automation. This paper reports design, fabrication, and control of an image guided automated non-prehensile magnetic micromanipulation system. The developed system consists of ferromagnetic microspheres (henceforth referred to as microbots) which are actuated via independently controlling currents in four solenoids placed in a quadrupole configuration. We use image based localization for determining the microbot and target cell locations. We developed feedback planner which invokes either of the two maneuvers, namely, push or align to move microbot in order to push the cell towards the goal location. Instead of customize microtools we use simple spherical shaped microbots for pushing target cells.

scholarly journals Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites

2019 ◽  
Author(s):  
Helen L. Miller ◽  
Sonia Contera ◽  
Adam J.M. Wollman ◽  
Adam Hirst ◽  
Katherine E. Dunn ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Single Molecule ◽  
Targeted Drug Delivery ◽  
Binding Sites ◽  
Dna Origami ◽  
Target Cells ◽  
Dna Nanostructures ◽  
Synthetic Dna ◽  
Drug Molecules ◽  
Targeted Drug

AbstractIntercalation of drug molecules into synthetic DNA nanostructures formed through self-assembled origami has been postulated as a valuable future method for targeted drug delivery. This is due to the excellent biocompatibility of synthetic DNA nanostructures, and high potential for flexible programmability including facile drug release into or near to target cells. Such favourable properties may enable high initial loading and efficient release for a predictable number of drug molecules per nanostructure carrier, important for efficient delivery of safe and effective drug doses to minimise non-specific release away from target cells. However, basic questions remain as to how intercalation-mediated loading depends on the DNA carrier structure. Here we use the interaction of dyes YOYO-1 and acridine orange with a tightly-packed 2D DNA origami tile as a simple model system to investigate intercalation-mediated loading. We employed multiple biophysical techniques including single-molecule fluorescence microscopy, atomic force microscopy, gel electrophoresis and controllable damage using low temperature plasma on synthetic DNA origami samples. Our results indicate that not all potential DNA binding sites are accessible for dye intercalation, which has implications for future DNA nanostructures designed for targeted drug delivery.

scholarly journals Formulation and Evaluation of Copper Nanoparticles Loaded Microsponges

2019 ◽  
Vol 11 (04) ◽  
Author(s):  
S. Kothai ◽  
R. Umamaheswari
Keyword(s):  
Copper Nanoparticles ◽  
Targeted Drug Delivery ◽  
Biomedical Applications ◽  
Entrapment Efficiency ◽  
Diffusion Method ◽  
Hibiscus Rosa Sinensis ◽  
Novel Approach ◽  
Targeted Drug ◽  
Antimicrobial And Antioxidant Activity ◽  
Emulsion Solvent Diffusion Method

Microsponges become imperative in the field of targeted drug delivery and in other biomedical applications. There was a clamant need for designing microsponges incorporating with green synthesised metal nanoparticles rather than the chemical drug in order to reduce the side effects of the drug and thus increasing the effectiveness of nature of the whole material. It provokes us to design this novel approach of loading copper nanoparticles into the microsponges. Here in this work, microsponges based on ethyl cellulose and polyvinyl alcohol were synthesised by Quasi-Emulsion Solvent diffusion method in which copper nanoparticles procured from Hibiscus rosa-sinensis leaf extract was incorporated. The Loaded microsponges were characterised by High Resolution Scanning Electron Microscopy (HR-SEM) and Particle size distribution Analyzer (PSA). The Drug content and Entrapment Efficiency of the microsponges were found out. The antimicrobial and antioxidant activity of the loaded microsponges were evaluated.

scholarly journals Sperm Cell Driven Microrobots—Emerging Opportunities and Challenges for Biologically Inspired Robotic Design

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 448 ◽  
Author(s):  
Ajay Singh ◽  
Mohammad Ansari ◽  
Mihir Mahajan ◽  
Shubhangi Srivastava ◽  
Shubham Kashyap ◽  
...  
Keyword(s):  
Targeted Drug Delivery ◽  
State Of The Art ◽  
Cell Manipulation ◽  
Small Scale ◽  
Sperm Cells ◽  
Biologically Inspired ◽  
New Class ◽  
Targeted Drug ◽  
New Applications ◽  
Coupling Mechanisms

With the advent of small-scale robotics, several exciting new applications like Targeted Drug Delivery, single cell manipulation and so forth, are being discussed. However, some challenges remain to be overcome before any such technology becomes medically usable; among which propulsion and biocompatibility are the main challenges. Propulsion at micro-scale where the Reynolds number is very low is difficult. To overcome this, nature has developed flagella which have evolved over millions of years to work as a micromotor. Among the microscopic cells that exhibit this mode of propulsion, sperm cells are considered to be fast paced. Here, we give a brief review of the state-of-the-art of Spermbots—a new class of microrobots created by coupling sperm cells to mechanical loads. Spermbots utilize the flagellar movement of the sperm cells for propulsion and as such do not require any toxic fuel in their environment. They are also naturally biocompatible and show considerable speed of motion thereby giving us an option to overcome the two challenges of propulsion and biocompatibility. The coupling mechanisms of physical load to the sperm cells are discussed along with the advantages and challenges associated with the spermbot. A few most promising applications of spermbots are also discussed in detail. A brief discussion of the future outlook of this extremely promising category of microrobots is given at the end.

scholarly journals Systemic Review of Biodegradable Nanomaterials in Nanomedicine

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 656 ◽  
Author(s):  
Shi Su ◽  
Peter M. Kang
Keyword(s):  
Targeted Drug Delivery ◽  
Biomedical Applications ◽  
Systemic Review ◽  
Advantages And Disadvantages ◽  
Literature Source ◽  
Target Sites ◽  
Different Types ◽  
Bio Imaging ◽  
Targeted Drug ◽  
Careful Design

Background: Nanomedicine is a field of science that uses nanoscale materials for the diagnosis and treatment of human disease. It has emerged as an important aspect of the therapeutics, but at the same time, also raises concerns regarding the safety of the nanomaterials involved. Recent applications of functionalized biodegradable nanomaterials have significantly improved the safety profile of nanomedicine. Objective: Our goal is to evaluate different types of biodegradable nanomaterials that have been functionalized for their biomedical applications. Method: In this review, we used PubMed as our literature source and selected recently published studies on biodegradable nanomaterials and their applications in nanomedicine. Results: We found that biodegradable polymers are commonly functionalized for various purposes. Their property of being naturally degraded under biological conditions allows these biodegradable nanomaterials to be used for many biomedical purposes, including bio-imaging, targeted drug delivery, implantation and tissue engineering. The degradability of these nanoparticles can be utilized to control cargo release, by allowing efficient degradation of the nanomaterials at the target site while maintaining nanoparticle integrity at off-target sites. Conclusion: While each biodegradable nanomaterial has its advantages and disadvantages, with careful design and functionalization, biodegradable nanoparticles hold great future in nanomedicine.

scholarly journals Targeted Drug Delivery and Image-Guided Therapy of Heterogeneous Ovarian Cancer Using HER2-Targeted Theranostic Nanoparticles

Theranostics ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 778-795 ◽  
Author(s):  
Minati Satpathy ◽  
Liya Wang ◽  
Rafal J. Zielinski ◽  
Weiping Qian ◽  
Y. Andrew Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
Image Guided Therapy ◽  
Image Guided ◽  
Theranostic Nanoparticles ◽  
Targeted Drug ◽  
Guided Therapy

Enhanced cellular uptake and tumor penetration of nanoparticles by imprinting the “hidden” part of membrane receptors for targeted drug delivery

2017 ◽  
Vol 53 (81) ◽  
pp. 11114-11117 ◽  
Author(s):  
Shan Peng ◽  
Yahua Wang ◽  
Na Li ◽  
Chong Li
Keyword(s):  
Drug Delivery ◽  
Cell Membrane ◽  
Cellular Uptake ◽  
Target Cell ◽  
Targeted Drug Delivery ◽  
Transmembrane Domain ◽  
Membrane Receptors ◽  
Tumor Penetration ◽  
Target Cell Membrane ◽  
Targeted Drug

Biting deep into the target cell membrane: nanoparticles targeting the transmembrane domain by trifluoroethanol-assisted epitope imprinting.

BIOMEDICAL APPLICATIONS OF MAGNETIC NANOPARTICLES

NANO ◽  
2010 ◽  
Vol 05 (05) ◽  
pp. 245-270 ◽  
Author(s):  
AIGUO WU ◽  
PING OU ◽  
LEYONG ZENG
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Nanoparticles ◽  
Targeted Drug Delivery ◽  
Biomedical Applications ◽  
Therapeutic Agents ◽  
Short Description ◽  
Resonance Imaging ◽  
Drug Delivery Carrier ◽  
Targeted Drug ◽  
Magnetic Resonance Imaging Mri

In this review, the applications of magnetic nanoparticles in biomedicine are summarized and introduced in three parts. (1) A short description of magnetic nanoparticles is explained. (2) Applications of magnetic nanoparticles in biomedicine are summarized. In biology, new progress of the magnetic separation techniques based on magnetic nanoparticles is discussed. In medicine, the magnetic nanoparticles as therapeutic agents (particularly as a hyperthermia agent, a targeted drug delivery carrier, and a magnetofection agent) as well as contrast agents in magnetic resonance imaging (MRI) are explained in detail. (3) A discussion and remarking conclusion of magnetic nanoparticles in biomedical applications are described. Finally, a perspective of the magnetic nanoparticles in biomedicine in future is also described.

Novel DNA Aptamers Against CCL21 Protein: Characterization and Biomedical Applications for Targeted Drug Delivery to T Cell-Rich Zones

2018 ◽  
Vol 28 (4) ◽  
pp. 242-251 ◽  
Author(s):  
Louis Chonco ◽  
Gerónimo Fernández ◽  
Rahul Kalhapure ◽  
María J. Hernáiz ◽  
Cecilia García-Oliva ◽  
...  
Keyword(s):  
Drug Delivery ◽  
T Cell ◽  
Targeted Drug Delivery ◽  
Biomedical Applications ◽  
Protein Characterization ◽  
Dna Aptamers ◽  
Targeted Drug
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