Heat Generation in Bone Cutting-Implications for Thermal Necrosis

2001 ◽  
Author(s):  
Debra Chenet Millon ◽  
Darren L. Hitt ◽  
Stephan J. LaPointe
Keyword(s):  
Bone Cells ◽  
Heat Exposure ◽  
Fibrous Tissue ◽  
Metatarsal Head ◽  
Bone Cell ◽  
Frictional Heat ◽  
Fat Cell ◽  
Critical Temperatures ◽  
Cell Degeneration ◽  
Prolonged Heat

Abstract A bunion is a common foot disorder caused by an abnormal outward projection of the joint and inward turning of the toe. Surgery to correct the malformation involves cutting the first metatarsal head, repositioning and setting it; the bone is then left to heal itself over time. A potentially serious by-product of the bone cutting is the frictional heat generated. While the heat susceptibility of individual bone cells varies throughout bone and is difficult to quantify, studies have shown that when injured, bone may not always heal as bone but rather as a fibrous tissue of varying degrees of differentiation. Prolonged heat exposure at or above critical temperatures may also lead to fat and bone cell resorption, a subsequent fat cell degeneration of the tissue, local swelling of cells as well as denaturation of the enzymatic and membrane proteins (Eriksson & Albrektsson, 1983, Li et al, 1999).

Effect of K-wire Reuse and Drill Mode on Heat Generation in Bone

Hand ◽  
2021 ◽  
pp. 155894472110031
Author(s):  
Muturi G. Muriuki ◽  
Arun K. Reddy ◽  
Alex Tauchen ◽  
Robert M. Havey ◽  
Avinash G. Patwardhan ◽  
...  
Keyword(s):  
Cell Death ◽  
Cortical Bone ◽  
Heat Generation ◽  
Kirschner Wire ◽  
Heat Exposure ◽  
Critical Temperatures ◽  
Rotary Drilling ◽  
External Cooling ◽  
K Wires ◽  
Oscillating Mode

Background We examined the effect of Kirschner wire (K-wire) reuse and use of oscillating mode on heat generation within cortical bone. Methods Two trocar-tipped K-wires were drilled through the diaphysis of each of 30 human metacarpals and phalanges: one K-wire was inserted in rotary mode and another in oscillating mode. Each wire was reused once. Thermocouples placed within the dorsal and volar bone adjacent to the K-wire drill path measured temperatures throughout each test. Results Peak cortex temperatures were 25°C to 164°C. Rotary drilling achieves peak temperatures quicker (31 ± 78 seconds vs 44 ± 78 seconds, P = .19) than oscillating drilling, but insertion time is also less, resulting in lower overall heat exposure. This effect is also seen when the K-wire is reused (34 ± 70 seconds vs 41 ± 85 seconds, P = .4). The length of time that cortical bone was exposed to critical temperatures (47°C or more) was significantly higher when a wire was reused (36 ± 72 seconds vs 43 ± 82 seconds, P = .008). Peak temperatures greater than 70°C (a temperature associated with instantaneous cell death) were observed on many occasions. Conclusions Overall heat exposure may be higher if a K-wire is reused or inserted in oscillating mode. In the absence of external cooling, K-wire insertion into cortical bone can easily expose bone to temperatures that exceed 70°C and may increase the risk of osteonecrosis.

scholarly journals The Development of Molecular Biology of Osteoporosis

2021 ◽  
Vol 22 (15) ◽  
pp. 8182
Author(s):  
Yongguang Gao ◽  
Suryaji Patil ◽  
Jingxian Jia
Keyword(s):  
Bone Resorption ◽  
Molecular Biology ◽  
Bone Formation ◽  
Bone Mass ◽  
Bone Remodeling ◽  
Bone Cells ◽  
Cell Activity ◽  
Bone Cell ◽  
Bone Disorders ◽  
Molecular Aspects

Osteoporosis is one of the major bone disorders that affects both women and men, and causes bone deterioration and bone strength. Bone remodeling maintains bone mass and mineral homeostasis through the balanced action of osteoblasts and osteoclasts, which are responsible for bone formation and bone resorption, respectively. The imbalance in bone remodeling is known to be the main cause of osteoporosis. The imbalance can be the result of the action of various molecules produced by one bone cell that acts on other bone cells and influence cell activity. The understanding of the effect of these molecules on bone can help identify new targets and therapeutics to prevent and treat bone disorders. In this article, we have focused on molecules that are produced by osteoblasts, osteocytes, and osteoclasts and their mechanism of action on these cells. We have also summarized the different pharmacological osteoporosis treatments that target different molecular aspects of these bone cells to minimize osteoporosis.

Morphological studies of bone and tendon

1992 ◽  
Vol 73 (2) ◽  
pp. S10-S13 ◽  
Author(s):  
S. B. Doty ◽  
E. R. Morey-Holton ◽  
G. N. Durnova ◽  
A. S. Kaplansky
Keyword(s):  
Electron Microscopy ◽  
Bone Cells ◽  
Weight Bearing ◽  
Light And Electron Microscopy ◽  
Cell Activity ◽  
Bone Cell ◽  
Electron Microscopic ◽  
Adult Rats ◽  
Morphological Studies ◽  
Metatarsal Bones

The Soviet biosatellite COSMOS 2044 carried adult rats on a spaceflight that lasted 13.8 days and was intended to repeat animal studies carried out on COSMOS 1887. Skeletal tissue and tendon from animals flown on COSMOS 2044 were studied by light and electron microscopy, histochemistry, and morphometric techniques. Studies were confined to the bone cells and vasculature from the weight-bearing tibias. Results indicated that vascular changes at the periosteal and subperiosteal region of the tibia were not apparent by light microscopy or histochemistry. However, electron microscopy indicated that vascular inclusions were present in bone samples from the flight animals. A unique combination of microscopy and histochemical techniques indicated that the endosteal osteoblasts from this same mid-diaphyseal region demonstrated a slight (but not statistically significant) reduction in bone cell activity. Electron-microscopic studies of the tendons from metatarsal bones showed a collagen fibril disorganization as a result of spaceflight. Thus changes described for COSMOS 1887 were present in COSMOS 2044, but the changes ascribed to spaceflight were not as evident.

scholarly journals Minireview: Transcriptional Regulation in Development of Bone

Endocrinology ◽  
2005 ◽  
Vol 146 (3) ◽  
pp. 1012-1017 ◽  
Author(s):  
Tatsuya Kobayashi ◽  
Henry Kronenberg
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Transcription Factors ◽  
Bone Cells ◽  
Genetic Manipulation ◽  
Bone Development ◽  
Regulation Of Gene Expression ◽  
Bone Cell ◽  
Cellular Functions ◽  
Multiple Differentiation

Regulation of gene expression by transcription factors is one of the major mechanisms for controlling cellular functions. Recent advances in genetic manipulation of model animals has allowed the study of the roles of various genes and their products in physiological settings and has demonstrated the importance of specific transcription factors in bone development. Three lineages of bone cells, chondrocytes, osteoblasts, and osteoclasts, develop and differentiate according to their distinct developmental programs. These cells go through multiple differentiation stages, which are often regulated by specific transcription factors. In this minireview, we will discuss selected transcription factors that have been demonstrated to critically affect bone cell development. Further study of these molecules will lead to deeper understanding in mechanisms that govern development of bone.

scholarly journals Protocol of Co-Culture of Human Osteoblasts and Osteoclasts to Test Biomaterials for Bone Tissue Engineering

2022 ◽  
Vol 5 (1) ◽  
pp. 8
Author(s):  
Giorgia Borciani ◽  
Giorgia Montalbano ◽  
Nicola Baldini ◽  
Chiara Vitale-Brovarone ◽  
Gabriela Ciapetti
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Cells ◽  
Bone Cell ◽  
Seeding Density ◽  
Bone Homeostasis ◽  
Bone Microenvironment

New biomaterials and scaffolds for bone tissue engineering (BTE) applications require to be tested in a bone microenvironment reliable model. On this assumption, the in vitro laboratory protocols with bone cells represent worthy experimental systems improving our knowledge about bone homeostasis, reducing the costs of experimentation. To this day, several models of the bone microenvironment are reported in the literature, but few delineate a protocol for testing new biomaterials using bone cells. Herein we propose a clear protocol to set up an indirect co-culture system of human-derived osteoblasts and osteoclast precursors, providing well-defined criteria such as the cell seeding density, cell:cell ratio, the culture medium, and the proofs of differentiation. The material to be tested may be easily introduced in the system and the cell response analyzed. The physical separation of osteoblasts and osteoclasts allows distinguishing the effects of the material onto the two cell types and to evaluate the correlation between material and cell behavior, cell morphology, and adhesion. The whole protocol requires about 4 to 6 weeks with an intermediate level of expertise. The system is an in vitro model of the bone remodeling system useful in testing innovative materials for bone regeneration, and potentially exploitable in different application fields. The use of human primary cells represents a close replica of the bone cell cooperation in vivo and may be employed as a feasible system to test materials and scaffolds for bone substitution and regeneration.

scholarly journals Energy Metabolism of Bone

2017 ◽  
Vol 45 (7) ◽  
pp. 887-893 ◽  
Author(s):  
Katherine J. Motyl ◽  
Anyonya R. Guntur ◽  
Adriana Lelis Carvalho ◽  
Clifford J. Rosen
Keyword(s):  
Bone Remodeling ◽  
Energy Homeostasis ◽  
Bone Structure ◽  
Bone Cells ◽  
Bone Cell ◽  
Whole Body ◽  
Blood Calcium ◽  
Micro Cracks ◽  
Intimate Link ◽  
Body Energy

Biological processes utilize energy and therefore must be prioritized based on fuel availability. Bone is no exception to this, and the benefit of remodeling when necessary outweighs the energy costs. Bone remodeling is important for maintaining blood calcium homeostasis, repairing micro cracks and fractures, and modifying bone structure so that it is better suited to withstand loading demands. Osteoclasts, osteoblasts, and osteocytes are the primary cells responsible for bone remodeling, although bone marrow adipocytes and other cells may also play an indirect role. There is a renewed interest in bone cell energetics because of the potential for these processes to be targeted for osteoporosis therapies. In contrast, due to the intimate link between bone and energy homeostasis, pharmaceuticals that treat metabolic disease or have metabolic side effects often have deleterious bone consequences. In this brief review, we will introduce osteoporosis, discuss how bone cells utilize energy to function, evidence for bone regulating whole body energy homeostasis, and some of the unanswered questions and opportunities for further research in the field.

scholarly journals Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1305 ◽  
Author(s):  
Arturo Pujia ◽  
Cristina Russo ◽  
Samantha Maurotti ◽  
Roberta Pujia ◽  
Vincenzo Mollace ◽  
...  
Keyword(s):  
Bone Cells ◽  
Citrus Fruit ◽  
Cell Types ◽  
Bone Cell ◽  
In Vivo Studies ◽  
Bone Biology ◽  
High Concentration ◽  
Mineral Density ◽  
Differentiation Markers

Epidemiological studies show that fruit consumption may modulate bone mineral density. However, data regarding the effect of the Citrus bergamia Risso (Bergamot orange), a citrus fruit containing a high concentration of flavonoids, on bone health are still lacking. In this study, we investigated the effects of Bergamot polyphenols on the Wnt/β-catenin pathway in two distinct bone cell types (Saos-2 and MG63). Findings showed that exposure to 0.01 and 0.1 mg/mL doses upregulate β-catenin expression (p = 0.001), osteoblast differentiation markers (e.g., RUNX2 and COL1A), and downregulate RANKL (p = 0.028), as compared to the control. Our results highlight, for the first time, that Bergamot polyphenols act on bone cells through the β-catenin pathway. In vivo studies are necessary to fully understand Bergamot’s role against bone resorption.

scholarly journals Origins of Alterations to Rankl Null Mutant Mouse Dental Root Development

2020 ◽  
Vol 21 (6) ◽  
pp. 2201
Author(s):  
Andrea Gama ◽  
Jorge William Vargas-Franco ◽  
Diana Carolina Sánchez Mesa ◽  
Elizabeth Restrepo Bedoya ◽  
Jérome Amiaud ◽  
...  
Keyword(s):  
Root Development ◽  
Alveolar Bone ◽  
Bone Cells ◽  
Cell Communication ◽  
Neutralizing Antibody ◽  
Bone Cell ◽  
Experimental Models ◽  
Nuclear Antigen ◽  
Bone Cell Differentiation ◽  
Proliferating Cell

The purpose of the present study was to assess the early stages of development of mouse first molar roots in the osteopetrotic context of RANKL invalidation in order to demonstrate that the radicular phenotype observed resulted not only from defective osteoclasts, but also from loss of cell-to-cell communication among dental, periodontium and alveolar bone cells involving RANKL signaling. Two experimental models were used in this study: Rankl mutants with permanent RANKL invalidation, and C57BL/6J mice injected during the first postnatal week with a RANKL neutralizing antibody corresponding to a transient RANKL invalidation. The dento-alveolar complex was systematically analyzed using micro-CT, and histological and immunohistochemical approaches. These experiments showed that the root elongation alterations observed in the Rankl-/- mice were associated with reduced proliferation of the RANK-expressing HERS cells with a significant decrease in proliferating cell nuclear antigen (PCNA) expression and a significant increase in P21 expression. The phenotypic comparison of the adult first molar root at 35 days between permanent and transitory invalidations of RANKL made it possible to demonstrate that alterations in dental root development have at least two origins, one intrinsic and linked to proliferation/differentiation perturbations in dental-root-forming cells, the other extrinsic and corresponding to disturbances of bone cell differentiation/function.

scholarly journals Intercellular calcium wave propagation in linear and circuit-like bone cell networks

2010 ◽  
Vol 368 (1912) ◽  
pp. 617-633 ◽  
Author(s):  
Bo Huo ◽  
Xin L. Lu ◽  
X. Edward Guo
Keyword(s):  
Wave Propagation ◽  
Gap Junctions ◽  
Calcium Release ◽  
Bone Cells ◽  
Molecular Transport ◽  
Bone Cell ◽  
Calcium Wave ◽  
Contact Printing ◽  
Inhibition Studies ◽  
Cell Networks

In the present study, the mechanism of intercellular calcium wave propagation in bone cell networks was identified. By using micro-contact printing and self-assembled monolayer technologies, two types of in vitro bone cell networks were constructed: open-ended linear chains and looped hexagonal networks with precisely controlled intercellular distances. Intracellular calcium responses of the cells were recorded and analysed when a single cell in the network was mechanically stimulated by nano-indentation. The looped cell network was shown to be more efficient than the linear pattern in transferring calcium signals from cell to cell. This phenomenon was further examined by pathway-inhibition studies. Intercellular calcium wave propagation was significantly impeded when extracellular adenosine triphosphate (ATP) in the medium was hydrolysed. Chemical uncoupling of gap junctions, however, did not significantly decrease the transferred distance of the calcium wave in the cell networks. Thus, it is extracellular ATP diffusion, rather than molecular transport through gap junctions, that dominantly mediates the transmission of mechanically elicited intercellular calcium waves in bone cells. The inhibition studies also demonstrated that the mechanical stimulation-induced calcium responses required extracellular calcium influx, whereas the ATP-elicited calcium wave relied on calcium release from the calcium store of the endoplasmic reticulum.

Selected Contribution: Regulatory pathways involved in mechanical induction of c-fos gene expression in bone cells

2000 ◽  
Vol 89 (6) ◽  
pp. 2498-2507 ◽  
Author(s):  
M. A. Peake ◽  
L. M. Cooling ◽  
J. L. Magnay ◽  
P. B. M. Thomas ◽  
A. J. El Haj
Keyword(s):  
Bone Cells ◽  
Mechanical Load ◽  
Mechanical Strain ◽  
Collagen Type I ◽  
Bone Cell ◽  
Type I ◽  
Regulatory Pathways ◽  
Four Point Bending ◽  
Load Response ◽  
Factor C

The regulatory pathways involved in the rapid response of the AP-1 transcription factor, c- fos, to mechanical load in human primary osteoblast-like (HOB) cells and the human MG-63 bone cell line were investigated using a four-point bending model. HOB and MG-63 cells showed upregulation of c- fos expression on fibronectin and collagen type I substrates; however, MG-63 cells did not respond on laminin YIGSR substrates. Addition of cytochalasin D and Arg-Gly-Asp peptides during loading did not inhibit the response, whereas addition of β1-integrin antibodies inhibited the load response. The role of Ca2+ signaling has been demonstrated by blocking upregulation with addition of 2 mM EGTA, which chelates extracellular Ca2+, and gadolinium (10 μM), which inhibits stretch-activated channels. Addition of the Ca2+ ionophore A-23187 induced upregulation without loading; however, addition of nifedipine (10 μM), the L-type channel blocker, failed to prevent the load response. Inhibitors of downstream pathways indicated the involvement of protein kinase C. Our results demonstrate a key involvement of Ca2+ signaling pathways and integrin binding in the c- fos response to mechanical strain.

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