scholarly journals Redox Status in Women with Rheumathoid Arthritis

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Aleksandra Vranic ◽  
Aleksandra Antovic ◽  
Nevena Draginic ◽  
Marijana Andjic ◽  
Marko Ravic ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Cartilage Damage ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Redox Status ◽  
Female Population

Abstract The aim of this study was to assess oxidative status and to set baseline characteristics for female population with established rheumatoid arthritis. Total of 42 patients with rheumatoid arthritis and 48 age- and sex-matched controls were included in the study. Clinical examination was performed and assessed disease activity. Peripheral blood samples were used for all the assays. The markers of oxidative stress were assessed, including plasma levels of index of lipid peroxidation - thiobarbituric acid reactive substances, hydrogen peroxide, superoxide anion radical, nitrites and activity of superoxide dismutase, catalase and reduced glutathione levels as antioxidant parameters. In the patients group, levels of hydrogen peroxide and index of lipid peroxidation were higher than in controls. Patients with rheumatoid arthritis had decreased superoxide dismutase and catalase activity compared to healthy subjects. Interestingly, controls had higher levels of nitrites compared to patients. Patients showed a marked increase in reactive oxygen species formation and lipid peroxidation as well as decrease in the activity of antioxidant defense system leading to oxidative stress which may contribute to tissue and cartilage damage and hence to the chronicity of the disease.

scholarly journals Characteristics of lipid peroxidation processes and factors of the antioxidant defense system in chronic atrophic gastritis and gastric cancer

2022 ◽  
Vol 20 (4) ◽  
pp. 63-70
Author(s):  
O. V. Smirnova ◽  
V. V. Tsukanov ◽  
A. A. Sinyakov ◽  
O. L. Moskalenko ◽  
N. G. Elmanova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gastric Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Defense System ◽  
Glutathione S Transferase

Background. The problem of gastric cancer remains unresolved throughout the world, while chronic atrophic gastritis (CAG) increases the likelihood of its development by 15 times. In the Russian Federation, the incidence of gastric cancer (GC) is among the highest, with it prevailing among males. One of the leading mechanisms in molecular pathology of membranes is lipid peroxidation (LPO). The severity of oxidative membrane damage depends on concomitant diseases, contributing to emergence and progression of pathological processes and development of cancer. Currently, the problem of LPO is unsolved in biological systems.The aim of this study was to investigate the state of LPO and antioxidant defense system in CAG and GC. Materials and methods. The parameters were studied in 45 patients with CAG and 50 patients with GC. The control group included 50 practically healthy volunteers without gastrointestinal complaints, who did not have changes in the gastric mucosa according to the fibroesophagogastroduodenoscopy (FEGDS) findings.Results. In patients with CAG, an increase in malondialdehyde, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase was found in the blood plasma compared with the control group. In patients with CAG, lipid peroxidation was activated, and the malondialdehyde level increased by 3.5 times relative to normal values. At the same time, the body fought against oxidative stress by increasing the activity of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase. All patients with GC showed pronounced oxidative stress in the blood plasma in the form of a 45-fold increase in malondialdehyde. The activity of the main antioxidant enzyme superoxide dismutase was reduced in GC. Catalase was activated, which indicated pronounced oxidative stress, significant damage to blood vessels, and massive cell death. Glutathione-related enzymes (glutathione S-transferase and glutathione peroxidase) and the antioxidant protein ceruloplasmin were activated, which also indicated significant oxidative stress and severe intoxication in patients with GC.Conclusion. Depending on the stage and type of cancer, an in-depth study of lipid peroxidation and factors of the antioxidant defense system can be used to correct therapy and prevent cancer and can serve as markers of progression and prognosis in gastric cancer. 

scholarly journals Protective Effect of Dorema glabrum on Induced Oxidative Stress by Diazinon in Hippocampus of Rat

2019 ◽  
pp. 1-7
Author(s):  
Mina Adampourezare ◽  
Parisa Sistani ◽  
Homeira Hatami Nemati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Male Wistar Rats ◽  
Pre Treatment

Introduction: Diazinon (DZN) administration produces lipid peroxidation as an indicator of oxidative stress in the brain. Some medicinal plants such as Dorema glabrum has antioxidant properties, so can be used as an antioxidant that may protect neurons from oxidative stress. The aim of present study was to investigate the effect of D. glabrum against DZN-induced oxidative stress in hippocampus. Methods: Twenty-four adult male Wistar rats were used in this study. The rats randomly were divided into four groups including a control group, and two groups received different doses of D. glabrum (40 and 80 mg/kg) as pre-treatment for 21 days with DZN (100 mg/Kg) that was injected intraperitoneally (ip) in last day of D. glabrum usage, and one group received only DZN. Thiobarbituric acid reactive substances (TBARS), which are the indicators of lipid peroxidation, and the activities of antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase) were determined in the ratsʼ hippocampus. Results: Administration of DZN significantly increased TBARS levels and superoxide dismutase activity and decreased glutathione peroxidase activity but there were no significant changes in catalase activity in the hippocampus. Combined D. glabrum and DZN treatment, caused a significant increase in glutathione peroxidase, a significant decrease of TBARS and a significant decrease in superoxide dismutase and again no significant changes in catalase activity in the rats’ hippocampus when compared to the rats treated with DZN. Conclusion: Our study demonstrated that D. glabrum had an amelioratory effect on oxidative stress induced by DZN.

scholarly journals Oxidative Stress of Vanadium-Mediated Oxygen Free Radical Generation Stimulated by Aluminium on Human Erythrocytes

1998 ◽  
Vol 35 (2) ◽  
pp. 254-260 ◽  
Author(s):  
M A M Abou-Seif
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Osmotic Fragility ◽  
Thiobarbituric Acid ◽  
Oxygen Free Radical ◽  
Membrane Lipid ◽  
Human Erythrocytes ◽  
Membrane Lipid Peroxidation ◽  
Radical Generation

It has been suggested that aluminium stimulates vanadium-mediated superoxide radical generation. The oxidative stress of generated superoxide radicals on antioxidant enzyme activity, oxidation of NADH and NADPH, membrane lipid peroxidation and osmotic fragility in human red blood cells (RBC) was investigated. RBC were incubated with varying concentrations of vanadium and aluminium ions at 37°C for 2 h. RBC incubated with vanadium ions showed significantly increased superoxide dismutase and catalase activities, and oxidized NADH and NADPH concentrations compared with control RBC preparations. Erythrocyte lipid peroxidation was assessed by measuring thiobarbituric acid reactivity. RBC incubated with elevated levels of vanadium showed significantly increased membrane lipid peroxidation when compared with control RBC; it increased further on addition of aluminium. A significant positive correlation was observed between the extent of vanadium induced membrane lipid peroxidation and the osmotic fragility of treated RBC. In the presence of vanadium, aluminium stimulates superoxide dismutase and catalase activities, NADH and NADPH oxidation and membrane lipid peroxidation, as well as increasing osmotic fragility of human erythrocytes. The stimulatory effect of aluminium was dependent on concentration. These results may have implications for the mechanism of toxicity of aluminium and vanadium in haemodialysis patients.

scholarly journals STUDI PRELIMINARI TENTANG PENGARUH D-GALAKTOSA DALAM MENGINDUKSI STRES OKSIDATIF PADA MENCIT JANTAN GALUR OUTBRED FK USU

2019 ◽  
Vol 2 (1) ◽  
pp. 1-5
Author(s):  
Siti Sarah Bintang Sarah Bintang ◽  
Yahwardiah Siregar ◽  
Muhammad Ichwan
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Dna Damage ◽  
Superoxide Dismutase ◽  
Free Radicals ◽  
The Body ◽  
Blood Collection ◽  
Male Mice ◽  
North Sumatra

Abstract Oxidative stress occurs due to imbalance of free radicals over antioxidant level in the body. This condition causes lipid peroxidation and DNA damage. D-Galaktosa is The mechanism of oxidative stress induced by d-galactose occurs in the subcellular, especially in the brain's mitochondria. Increasing the concentration of d-galaktosa is oxidized by galaktosa oxidase to form hydrogen peroxide (H202) which causes a decrease in superoxide dismutase (SOD). H202 reacts with reduced iron and forms hydroxide ions (OH-). Objective: The aim of this study was to determine the effect of d-galaktosa induction on oxidative stress levels (MDA) in male mice. Methods: Methods of samples of mice given d-galaktosa and blood collection from the heart were carried out at the Pharmacology Laboratory of the Faculty of Medicine, University of North Sumatra. Results: The results showed that administration of d-galaktosa, through intraperitonial injection every day for 6 weeks, had an effect on the levels of oxidative stress in male mice. Conclusion: The results of this study indicate that administration of d-galaktosa, through intraperitonial injection every day for 6 weeks, has an effect on levels of oxidative stress in male mice.

scholarly journals Circulating lipid peroxidation, plasma and erythrocyte antioxidant status in patients with rheumatoid arthritis

2009 ◽  
Vol 35 (2) ◽  
pp. 57-62 ◽  
Author(s):  
Palanisamy Pasupathi ◽  
Mathiyalagan Deepa ◽  
P. Rani ◽  
R. Ramesh Sankar
Keyword(s):  
Rheumatoid Arthritis ◽  
Lipid Peroxidation ◽  
Healthy Subjects ◽  
Antioxidant Defense ◽  
Defense Mechanism ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reactive Protein ◽  
Species Production

The aim of the study was to investigate the levels of lipid peroxidation, and both plasma and erythrocyte antioxidant states in patients with rheumatoid arthritis. The population consisted of 60 subjects divided into two groups, 30 subjects had evidence of rheumatoid arthritis and age and sex matched healthy subjects were studied as controls. The level of plasma and erythrocyte thiobarbituric acid reactive substances (TBARS) was markedly increased in both the rheumatoid arthritis patients when compared to control subjects. The activities of plasma and erythrocyte antioxidants were significantly decreased in rheumatoid arthritis. C reactive protein, rheumatoid factor and antistreptolysin-O were significantly higher in rheumatoid arthritis patients than in healthy subjects. In conclusion, on the basis of enhanced lipid peroxidation in rheumatoid arthritis patients with concomitant failure of both the plasma, and erythrocyte antioxidants defense mechanism. These results are consistent with the underlying hypothesis that there is an  imbalance between reactive oxygen species production and the antioxidant defense system in inflammatory rheumatoid arthritis disease.Keywords: Antioxidant; Lipid peroxidation; Rheumatoid arthritisOnline: 13 August 2009DOI: 10.3329/bmrcb.v35i2.2798Bangladesh Med Res Counc Bull 2009; 35: 57-62

Analysis of Oxidative Stress Indicators in Polish Patients With Prostate Cancer

2021 ◽  
Author(s):  
Joanna Maria Drozdz-Afelt ◽  
Beata Barbara Koim-Puchowska ◽  
Piotr Kaminski
Keyword(s):  
Prostate Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Detoxification Enzymes ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Significant Difference

Abstract Aims: The aim of the study was to analyze the activity of antioxidant enzymes (glutathione S-transferase, catalase, superoxide dismutase) in order to determine the role of detoxification mechanisms in prostate cancer. The concentration of malondialdehyde, which is an indicator of lipid peroxidation in cancer patients, was also tested.Methods: The activities of superoxide dismutase (SOD), catalase CAT and glutathione S-transferase (GST ) were measured using ready-made kits; lipid peroxidation intensity was determined by the thiobarbituric acid method.Results: Superoxide dismutase was the only enzyme among antioxidant and detoxification enzymes for which a statistically significant difference in activity was found between the studied groups [1.4 U * ml-1 in patients vs. 1.6 U * ml-1 in control]. No statistically significant differences were found for two other biomarkers of antioxidant activity (GST, CAT). There were also no statistically significant differences in the concentration of MDA between the group of men with prostate cancer and the control group.Conclusion: The lower SOD activity in men with prostate cancer may be due to a deficiency in their antioxidant defense system.

Antioxidative Responses of Tobacco Expressing a Bacterial Glutathione Reductase

2003 ◽  
Vol 58 (11-12) ◽  
pp. 843-849 ◽  
Author(s):  
Barbara Lederer ◽  
Peter Böger
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Stress Response ◽  
Glutathione Reductase ◽  
Wild Type ◽  
Transcript Levels ◽  
E Coli ◽  
Violaxanthin Deepoxidase

Abstract Reports on stress response of tobacco expressing a bacterial glutathione reductase (GR) do not agree. To clarify this situation we investigated several parameters using the tobacco BelW3 line and its transformant BelW3gor expressing an E. coli GR. This alteration in the activity of GR led to an ambiguous modification of the antioxidative system. In contrast to the wild type, the transgenic tobacco suffered lipid peroxidation under moderate light intensities, while it was found to be more resistant towards oxidative stress induced by paraquat or hydrogen peroxide. Transcript levels for violaxanthin deepoxidase and cytosolic Cu-Zn-superoxide dismutase were strongly reduced in BelW3gor plants as compared to BelW3.

scholarly journals Effects of Nonylphenol-Induced Oxidative Stress in Ovary of Cichlid Fish, Etroplus maculatus (Bloch, 1795)

2016 ◽  
Vol 58 ◽  
pp. 11-15 ◽  
Author(s):  
K.P. Asifa ◽  
K.C. Chitra
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Cichlid Fish ◽  
Time Dependent ◽  
Dependent Manner ◽  
Treatment Groups ◽  
Hydrogen Peroxide Generation

The present study was designed to evaluate the effects of nonylphenol in the pro-oxidant/ antioxidant system in ovary of the cichlid fish Etroplus maculatus. Fishes were exposed at two sublethal concentrations (one-fifth and one-tenth of LC50) of nonylphenol for 24, 72 and 96 h maintaining control groups. The oxidative stress indices as the activities of antioxidant enzymes, superoxide dismutase, catalase, glutathione reductase along with the levels of hydrogen peroxide generation and lipid peroxidation were monitored in concentration-and time-dependent manner. Activity of superoxide dismutase significantly (P<0.05) increased at both concentrations in time-dependent manner. Meanwhile the activities of catalase and glutathione reductase significantly (P<0.05) decreased after 72 and 96 h of nonylphenol treatment. The levels of hydrogen peroxide generation and lipid peroxidation increased in all treatment groups when compared to controls. The present results demonstrated that the induction of oxidative stress in ovary of fish by the generation of lipid peroxidation could be due to the exposure of environmental contaminant, nonylphenol. Therefore, the observed oxidative stress in ovary can be indicated as a mechanism of toxicity in the fish exposed to nonylphenol.

scholarly journals Evidence for Chlordecone-Stimulated Oxidative Stress in Different Tissues of the Cichlid Fish, Pseudetroplus Maculatus (Bloch, 1795)

2017 ◽  
Vol 75 (2) ◽  
pp. 67-75
Author(s):  
Kunimmal Poothaadammal Asifa ◽  
Kumari Chidambaran Chitra
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Cichlid Fish ◽  
Gill Tissue ◽  
The Body ◽  
Marker Enzyme ◽  
Antioxidant Defence System

Abstract The present study was designed to assess the effect of chlordecone on the stimulation of oxidative stress in gill, liver and brain tissues of the cichlid fish, Pseudetroplus maculatus. Fishes were exposed to chlordecone at two sublethal concentrations, 3.5 and 7 μg/L, for 24, 72 and 96 h, maintaining ten animals in each group. Chlordecone treatment did not alter the body weight of the animal. However, significant increase in the secretion of mucous and decrease in the weights of gill, brain and hepatosomatic index were observed at 7 μg/L of chlordecone treatment only after 96 h. Gill tissue showed significant increase in the activities of superoxide dismutase, catalase and glutathione reductase along with elevated levels of hydrogen peroxide and lipid peroxidation in concentration and timedependent manner. This could be the defensive mechanism of gill tissue to escape the toxic effects of chlordecone. In the liver tissue, superoxide dismutase activity was increased by 39% at 3.5 μg/L and by 73% at 7 μg/L of chlordecone treatment. Activities of catalase and glutathione reductase were decreased 3 to 9 times at 3.5 and 7 μg/L concentrations, respectively, with concomitant increase in hydrogen peroxide generation (17 to 28 times) and lipid peroxidation (3 to 7 times) at the end of 96 h, which reveals the failure of hepatic antioxidant system to prevent free radical generation owing to chlordecone exposure. Activities of all antioxidant enzymes in the brain were inhibited by 29 to 80% along with the induction of hydrogen peroxide (13 to 20 times) and lipid peroxidation (6 to 11 times), thereby indicating imbalance in the antioxidant status. Activities of gill and liver marker enzyme - alkaline phosphatase - and acetylcholinesterase in brain were decreased. Therefore, imbalance in the antioxidant defence system as a result of chlordecone toxicity could lead to susceptible oxidative stress in various tissues of the fish.

scholarly journals Lipid Peroxidation, Antioxidative Defense and Level of 8-Hydroxy-2-Deoxyguanosine in Cervical Cancer Patients

2018 ◽  
Vol 37 (3) ◽  
pp. 336-345 ◽  
Author(s):  
Marija Jelić ◽  
Aljoša Mandić ◽  
Nebojša Kladar ◽  
Jan Sudji ◽  
Biljana Božin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Group Iv ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Group Iii ◽  
Group Ii

SummaryOxidative stress has been associated with cervical cancer. Our aim was to examine lipid peroxidation and the extent of oxidative stress in women diagnosed with different stages of cervical cancer in order to evaluate its potential role in the evolution of cancer. We measured the concentration of thiobarbituric acid reactive substances, activities of antioxidative enzymes and 8-hydroxy-2-deoxyguanosine in 153 subjects. Enzymatic activity as well as TBARS concentration were measured spectrophotometrically, while 8-OHdG was determined by gas chromatography-mass spectrometry. PPatients were categorized: group II H-SIL; group III FIGO Ia-Ib and group IV FIGO IIa-IV. Our results showed highly significant increase in the level of lipid peroxidation in group IV when com pared to the control group, group II and group III (p<0.001). Activity of superoxide dismutase was also significantly higher in group IV when compared to control group (p<0.01), group II (p<0.01) and group III (p<0.05). Activity of catalase was also significantly higher in group IV when compared to control group (p<0.005), group II (p<0.005) and group III (p<0.05). Activity of glutathione-S-transferase was also significantly higher in group IV when compared to control group (p<0.05), group II (p<0.05) and group III (p<0.05). Activities of glutathione peroxidase and glutathione reductase showed no significant differences among the groups. Level of 8-OHdG was significantly higher in group IV than in the other groups (p<0.01). It can be concluded that oxidative stress is possibly involved in the pathogenesis of cervical cancer, demonstrated by increased lipid peroxidation and an altered antioxidant defense system and higher levels of 8-OHdG.

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