scholarly journals Prevalence and Genetic Diversity of Human Sapoviruses in Shellfish from Commercial Production Areas in Galicia, Spain

2015 ◽  
Vol 82 (4) ◽  
pp. 1167-1172 ◽  
Author(s):  
Miguel F. Varela ◽  
David Polo ◽  
Jesús L. Romalde
Keyword(s):  
Reverse Transcription ◽  
Gene Sequence ◽  
Content Type ◽  
Viral Contamination ◽  
The North ◽  
Class B ◽  
Capsid Gene Sequence ◽  
Contamination Levels ◽  
The One ◽  
Production Areas

ABSTRACTThe prevalence of human forms ofSapovirus, an emerging pathogen of human gastroenteritis, was investigated in an 18-month survey from class B mollusc-harvesting areas in two Galician rias (northwest Spain). The detection and quantification ofSapoviruswas performed by reverse transcription-real-time PCR, according to the recently developed standard method ISO/TS 15216-1:2013, and genotyping by reverse transcription-nested PCR. The bivalve species studied were wild and cultured mussels (Mytilus galloprovincialis), clams (Venerupis philippinarumandVenerupis decussata), and cockles (Cerastoderma edule).Sapoviruswas detected in 30 out of 168 samples (17.9%), with cockles being the species with the highest prevalence of positives (28.1%), followed by clams (22.6%), wild mussels (14.3%), and cultured mussels (12.9%). The estuary in the south of the region demonstrated a higher percentage of positive samples (21.8%) than the one in the north (14.4%). Viral contamination levels for the positive samples ranged between 1.9 × 103and 1.4 × 105RNA copies/g of digestive tissue. ThirteenSapovirussequences could be obtained based on partial capsid gene sequence and were classified into four genotypes: GI.1 (2 samples), GI.2 (8 samples), GIV.1 (2 samples), and GV.1 (1 sample).

scholarly journals Is ‘co’ in coworking a short for contradictions?

2021 ◽  
Vol 17 (5) ◽  
pp. 38-63
Author(s):  
Silvia Ivaldi ◽  
Annalisa Sannino ◽  
Giuseppe Scaratti
Keyword(s):  
Historical Development ◽  
Design Methodology ◽  
Two Dimensions ◽  
Content Type ◽  
The North ◽  
Dialectical Approach ◽  
History Of ◽  
Coworking Spaces ◽  
Cultural Historical Activity ◽  
The One

PurposeBuilding on the existing literature and on a series of interviews conducted in very diverse coworking spaces, this article attempts at analyzing coworking by focusing on the historical evolution and heterogeneity of its interpretations, as well as the plurality of its realization in practice and prospective developments.Design/methodology/approachThe theoretical framework adopted is Cultural Historical Activity Theory – a dialectical approach which allows the study of human activities as historically evolving and complex systems which change under the impulse of their inner contradictions. The analysis presented here starts with an overview of the history of the theoretical elaborations and discussions of coworking. The authors then focus on the experiences and interpretations of this phenomenon as conveyed by coworkers and coworking managers in the north of Italy – one of the most active coworking areas in Europe.FindingsCoworking first emerged as a way of promoting forms of work and organization that require simultaneous, multidirectional, and reciprocal work, as understood in contrast to forms that incorporate an established division of labor, demarcated communities, and formal and informal sets of rules. However, with time, coworking has evolved toward novel directions, giving rise to heterogeneous interpretations of it. Inquiry constitutes a deeper investigation of the heterogeneity of coworking. The take-away message here is that the prefix co- in coworking can be interpreted, through a play of words, to evoke multiple positions and views conveying internal contradictions.Originality/valueThe historical overview of coworking shows a strong differentiation and multisided interpretation of this phenomenon along two dimensions of historical development, namely, social and business, and outward and inward. The qualitative analysis of the interviews traces the different lived interpretations and conceptions of coworking. The analysis confirms, on the one hand, the complexity and heterogeneity described in the literature, and on the other hand, it enriches the literature by depicting the contradictory nature of the phenomenon, including how the historical and inner tensions of coworking are dynamically evolving in the concrete experiences reported by the managers and users in the coworking spaces.

scholarly journals One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay

2020 ◽  
Author(s):  
Deguo Wang
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Prevention And Control ◽  
Gene Sequence ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
N Gene ◽  
One Pot ◽  
The One ◽  
And Control

AbstractBackgroundRapid and reliable diagnostic assays were critical for prevention and control of the coronavirus pneumonia caused by COVID-19.ObjectiveThis study was to establish one-pot real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay and one-pot visual RT-LAMP assay for the detection of COVID-19.MethodsSix specific LAMP primers targeting the N gene of COVID-19 were designed, the RT-LAMP reaction system was optimized with plasmid pUC57 containing N gene sequence, the detection limit was determined with a serial dilution of the plasmid pUC57 containing N gene sequence, and the one-pot real-time RT-LAMP assay and one-pot visual RT-LAMP assay for the detection of COVID-19 were established.ResultsOur results showed that the one-pot RT-LAMP assays can detect COVID-19 with a limit of ≥ 6 copies per μl−1 of pUC57 containing N gene sequence.ConclusionThis study provides rapid, reliable and sensitive tools for facilitating preliminary and cost-effective prevention and control of COVID-19.

scholarly journals Novosphingobium fuchskuhlense sp. nov., isolated from the north-east basin of Lake Grosse Fuchskuhle

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 586-592 ◽  
Author(s):  
Stefanie P. Glaeser ◽  
Kathrin Bolte ◽  
Karin Martin ◽  
Hans-Jürgen Busse ◽  
Hans-Peter Grossart ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
North East ◽  
The North ◽  
Type Strains

A yellow pigmented, Gram-negative, rod-shaped bacterium designated FNE08-7T was isolated from subsurface water of the north-east basin of the bog lake Grosse Fuchskuhle (Brandenburg, Germany). A first analysis of the nearly full-length 16S rRNA gene sequence analysis including environmental 16S rRNA gene sequences derived from freshwater ecosystems showed that strain FNE08-7T is the first cultured representative, to our knowledge, of the freshwater tribe Novo-A2. Further analysis indicates highest 16S rRNA gene sequence similarities to the type strains of Novosphingobium stygium (98.0 %) and Novosphingobium taihuense (97.4 %) and between 94.0 % and 96.9 % sequence similarity to other members of the genus Novosphingobium . Reconstruction of phylogenetic trees showed that strain FNE08-7T formed a distinct cluster with the type strains of N. stygium and N. taihuense supported by high bootstrap values. DNA–DNA hybridization of strain FNE08-7T with N. stygium SMCC B0712T and N. taihuense DSM 17507T revealed low similarity values of 18.4 % (reciprocal: 11.4 %) and 23.1 % (reciprocal: 54.2 %), respectively. The predominant fatty acid of the isolate is C18 : 1ω7c (56.4 %) and two characteristic 2-hydroxy fatty acids, C14 : 0 2-OH (16.5 %) and C15 : 0 2-OH (3.3 %) occur. Ubiquinone Q-10 is the major respiratory quinone. The predominant polar lipids are phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine and minor amounts of diphosphatidylglycerol. Spermidine is the predominant polyamine. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicate that strain FNE08-7T represents a novel species of the genus Novosphingobium within the Alphaproteobacteria . Therefore, we propose the species Novosphingobium fuchskuhlense sp. nov., with FNE08-7T ( = DSM 25065T = CCM 7978T = CCUG 61508T) as the type strain.

Mexican regional inequalities will rise despite SEZs

2015 ◽  
Keyword(s):  
Focal Point ◽  
Left Wing ◽  
Content Type ◽  
Southern States ◽  
Regional Inequalities ◽  
The North ◽  
North American Market ◽  
Central Regions ◽  
The Government ◽  
The One

Subject Regional inequalities in Mexico. Significance Regional differences within Mexico, notably between the northern and central regions on the one hand, and southern states on the other, show no sign of diminishing. Rather, divergence seems set to continue as the country evolves into a manufacturing powerhouse focused on the North American market. Impacts The government lacks financial resources, especially given low oil prices, to mount an aggressive industrial policy to develop poor states. Poverty in Guerrero, Oaxaca and Chiapas makes them a continuing focal point for left-wing radical activism. No auto industry investments should be expected in the southern states, even with the incentive of the SEZs.

MBAH HENDRO KUSUMO BIN SYECH AHMAD MUTAMAKKIN: JEJAK SYIAR ISLAM DI WILAYAH PATI

EMPIRISMA ◽  
2008 ◽  
Vol 27 (2) ◽  
Author(s):  
Fathimatuz Zahra Dan Abdul Azis
Keyword(s):  
Central Region ◽  
Marital Relationship ◽  
Southern Region ◽  
North Coast ◽  
The North ◽  
Trace Back ◽  
Northern Regions ◽  
The One

Pati is a region on the north coast, according to the hypothesis of the researcher, the region is divided into three categories. The northern regions are more religious, the central is more plural, while the southern region is in the middle. In the central region there are many relics of tombs believed to be the those of the Muslim proselytizers in the area of Pati. The one that attracts the researcher is a tomb in the Gambiran area, where there are five local Muslim saints buried, one of them belons to mbah Hendro Kusumo, the son of Syech Ahmad Mutamakkin. This article attempts to trace back the spreading of Islam in Pati based on the existence of thetomb of Mbah Hendro Kusumo. It wants to answer question of whethere the existence of his tomb is due to his studying there or marital relationship, and how it relates to the spreading of Islam.Keywords: Mbah Hendro Kusumo, Traces of Islamic Dakwah, Islam

Preaching and Sermons

2017 ◽  
Author(s):  
Robert H. Ellison
Keyword(s):  
Eighteenth Century ◽  
North Atlantic ◽  
Religious Revival ◽  
Early Nineteenth Century ◽  
Denominational Identity ◽  
The North ◽  
The North Atlantic ◽  
The One ◽  
The Impact

Prompted by the convulsions of the late eighteenth century and inspired by the expansion of evangelicalism across the North Atlantic world, Protestant Dissenters from the 1790s eagerly subscribed to a millennial vision of a world transformed through missionary activism and religious revival. Voluntary societies proliferated in the early nineteenth century to spread the gospel and transform society at home and overseas. In doing so, they engaged many thousands of converts who felt the call to share their experience of personal conversion with others. Though social respectability and business methods became a notable feature of Victorian Nonconformity, the religious populism of the earlier period did not disappear and religious revival remained a key component of Dissenting experience. The impact of this revitalization was mixed. On the one hand, growth was not sustained in the long term and, to some extent, involvement in interdenominational activity undermined denominational identity; on the other hand, Nonconformists gained a social and political prominence they had not enjoyed since the middle of the seventeenth century and their efforts laid the basis for the twentieth-century explosion of evangelicalism in Africa, Asia, and South America.

How Phillips saw the light

2020 ◽  
Vol 36 (8) ◽  
pp. 29-31
Keyword(s):  
Design Methodology ◽  
Reading Time ◽  
Daily Basis ◽  
Business World ◽  
Content Type ◽  
Freak Show ◽  
The World ◽  
Pertinent Information ◽  
The One ◽  
Practical Implications

Purpose Reviews the latest management developments across the globe and pinpoints practical implications from cutting-edge research and case studies. Design/methodology/approach This briefing is prepared by an independent writer who adds their own impartial comments and places the articles in context. Findings The problem with developing a reputation of being something of an oracle in the business world is that all of a sudden, everyone expects you to pull off the trick of interpreting the future on a daily basis. Like a freak show circus act or one-hit wonder pop singer, people expect you to perform when they see you, and they expect you to perform the thing that made you famous, even if it is the one thing in the world you don’t want to do. And when you fail to deliver on these heightened expectations, you are dismissed as a one trick pony, however good that trick is in the first place. Originality/value The briefing saves busy executives and researchers hours of reading time by selecting only the very best, most pertinent information and presenting it in a condensed and easy-to-digest format.

PROMETHEE-based ranking of project managers based on the five personality traits

Kybernetes ◽  
2019 ◽  
Vol 49 (4) ◽  
pp. 1083-1102
Author(s):  
Georgios N. Aretoulis ◽  
Jason Papathanasiou ◽  
Fani Antoniou
Keyword(s):  
Personality Traits ◽  
Questionnaire Survey ◽  
Multicriteria Decision Making ◽  
Project Managers ◽  
Preference Ranking ◽  
Infrastructure Projects ◽  
Public Infrastructure ◽  
Content Type ◽  
The One ◽  
Selection Of

Purpose This paper aims to rank and identify the most efficient project managers (PMs) based on personality traits, using Preference Ranking Organization METHod for Enrichment Evaluations (PROMETHEE) methodology. Design/methodology/approach The proposed methodology relies on the five personality traits. These were used as the selection criteria. A questionnaire survey among 82 experienced engineers was used to estimate the required weights per personality trait. A second two-part questionnaire survey aimed at recording the PMs profile and assess the performance of personality traits per PM. PMs with the most years of experience are selected to be ranked through Visual PROMETHEE. Findings The findings suggest that a competent PM is the one that scores low on the “Neuroticism” trait and high especially on the “Conscientiousness” trait. Research limitations/implications The research applied a psychometric test specifically designed for Greek people. Furthermore, the proposed methodology is based on the personality characteristics to rank the PMs and does not consider the technical skills. Furthermore, the type of project is not considered in the process of ranking PMs. Practical implications The findings could contribute in the selection of the best PM that maximizes the project team’s performance. Social implications Improved project team communication and collaboration leading to improved project performance through better communication and collaboration. This is an additional benefit for the society, especially in the delivery of public infrastructure projects. A lot of public infrastructure projects deviate largely as far as cost and schedule is concerned and this is an additional burden for public and society. Proper project management through efficient PMs would save people’s money and time. Originality/value Identification of the best PMbased on a combination of multicriteria decision-making and psychometric tests, which focus on personality traits.

scholarly journals Thinner Sea Ice Contribution to the Remarkable Polynya Formation North of Greenland in August 2018

2021 ◽  
Author(s):  
Xiaoyi Shen ◽  
Chang-Qing Ke ◽  
Bin Cheng ◽  
Wentao Xia ◽  
Mengmeng Li ◽  
...  
Keyword(s):  
Sea Ice ◽  
Ice Cover ◽  
Reanalysis Data ◽  
Mean Value ◽  
Ocean Model ◽  
North Coast ◽  
Average Value ◽  
The North ◽  
Wind Sea ◽  
The One

AbstractIn August 2018, a remarkable polynya was observed off the north coast of Greenland, a perennial ice zone where thick sea ice cover persists. In order to investigate the formation process of this polynya, satellite observations, a coupled ice-ocean model, ocean profiling data, and atmosphere reanalysis data were applied. We found that the thinnest sea ice cover in August since 1978 (mean value of 1.1 m, compared to the average value of 2.8 m during 1978–2017) and the modest southerly wind caused by a positive North Atlantic Oscillation (mean value of 0.82, compared to the climatological value of −0.02) were responsible for the formation and maintenance of this polynya. The opening mechanism of this polynya differs from the one formed in February 2018 in the same area caused by persistent anomalously high wind. Sea ice drift patterns have become more responsive to the atmospheric forcing due to thinning of sea ice cover in this region.

scholarly journals Faecalicoccus acidiformans gen. nov., sp. nov., isolated from the chicken caecum, and reclassification of Streptococcus pleomorphus (Barnes et al. 1977), Eubacterium biforme (Eggerth 1935) and Eubacterium cylindroides (Cato et al. 1974) as Faecalicoccus pleomorphus comb. nov., Holdemanella biformis gen. nov., comb. nov. and Faecalitalea cylindroides gen. nov., comb. nov., respectively, within the family Erysipelotrichaceae

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3877-3884 ◽  
Author(s):  
Celine De Maesschalck ◽  
Filip Van Immerseel ◽  
Venessa Eeckhaut ◽  
Siegrid De Baere ◽  
Margo Cnockaert ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Strains LMG 27428T and LMG 27427 were isolated from the caecal content of a chicken and produced butyric, lactic and formic acids as major metabolic end products. The genomic DNA G+C contents of strains LMG 27428T and LMG 27427 were 40.4 and 38.8 mol%. On the basis of 16S rRNA gene sequence similarity, both strains were most closely related to the generically misclassified Streptococcus pleomorphus ATCC 29734T. Strain LMG 27428T could be distinguished from S. pleomorphus ATCC 29734T based on production of more lactic acid and less formic acid in M2GSC medium, a higher DNA G+C content and the absence of activities of acid phosphatase and leucine, arginine, leucyl glycine, pyroglutamic acid, glycine and histidine arylamidases, while strain LMG 27428 was biochemically indistinguishable from S. pleomorphus ATCC 29734T. The novel genus Faecalicoccus gen. nov. within the family Erysipelotrichaceae is proposed to accommodate strains LMG 27428T and LMG 27427. Strain LMG 27428T ( = DSM 26963T) is the type strain of Faecalicoccus acidiformans sp. nov., and strain LMG 27427 ( = DSM 26962) is a strain of Faecalicoccus pleomorphus comb. nov. (type strain LMG 17756T = ATCC 29734T = DSM 20574T). Furthermore, the nearest phylogenetic neighbours of the genus Faecalicoccus are the generically misclassified Eubacterium cylindroides DSM 3983T (94.4 % 16S rRNA gene sequence similarity to strain LMG 27428T) and Eubacterium biforme DSM 3989T (92.7 % 16S rRNA gene sequence similarity to strain LMG 27428T). We present genotypic and phenotypic data that allow the differentiation of each of these taxa and propose to reclassify these generically misnamed species of the genus Eubacterium formally as Faecalitalea cylindroides gen. nov., comb. nov. and Holdemanella biformis gen. nov., comb. nov., respectively. The type strain of Faecalitalea cylindroides is DSM 3983T = ATCC 27803T = JCM 10261T and that of Holdemanella biformis is DSM 3989T = ATCC 27806T = CCUG 28091T.

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