scholarly journals Mycobacterium avium Subspecies paratuberculosis Cultured from Locally and Commercially Pasteurized Cow's Milk in the Czech Republic

2005 ◽  
Vol 71 (3) ◽  
pp. 1210-1214 ◽  
Author(s):  
Wuhib Y. Ayele ◽  
Petra Svastova ◽  
Petr Roubal ◽  
Milan Bartos ◽  
Ivo Pavlik
Keyword(s):  
Czech Republic ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Raw Milk ◽  
The United States ◽  
Cow’S Milk ◽  
Fecal Culture ◽  
The Czech Republic ◽  
Cow's Milk ◽  
Culture Positive

ABSTRACT Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7°C for 15 s in a local pasteurization unit were also obtained from two dairy herds, designated herds A and B, with low and high levels, respectively, of subclinical Mycobacterium avium subsp. paratuberculosis infection, and from one herd, herd C, without infection. Infection in individual cows in each herd was tested by fecal culturing. Milk samples were brought to the Veterinary Research Institute in Brno, Czech Republic, processed, inoculated onto Herrold's egg yolk slants, and incubated for 32 weeks. Colonies were characterized by morphology, Ziehl-Neelsen staining, mycobactin J dependency, and IS900 PCR results. M. avium subsp. paratuberculosis was cultured from 4 of 244 units (1.6%) of commercially pasteurized retail milk. M. avium subsp. paratuberculosis was also cultured from 2 of 100 (2%) cartons of locally pasteurized milk derived from infected herds A and B and from 0 of 100 cartons of milk from uninfected herd C. Raw milk from 1 of 10 (10%) fecal culture-positive cows in herd A and from 13 of 66 (19.7%) fecal culture-positive cows in herd B was culture positive for M. avium subsp. paratuberculosis. These findings confirm that M. avium subsp. paratuberculosis is present in raw milk from subclinically infected dairy cows. The culture of M. avium subsp. paratuberculosis in the Czech Republic from retail milk that had been pasteurized locally or commercially to the required national and European Union standards is in agreement with similar research on milk destined for consumers in the United Kingdom and the United States and shows that humans are being exposed to this chronic enteric pathogen by this route.

Assessment of the Colorimetric and Fluorometric Assays for Alkaline Phosphatase Activity in Cow's, Goat's, and Sheep's Milk

2008 ◽  
Vol 71 (9) ◽  
pp. 1884-1888 ◽  
Author(s):  
V. KLOTZ ◽  
ART HILL ◽  
K. WARRINER ◽  
M. GRIFFITHS ◽  
J. ODUMERU
Keyword(s):  
Alkaline Phosphatase ◽  
Colorimetric Assay ◽  
Residual Activity ◽  
Raw Milk ◽  
The United States ◽  
Cow’S Milk ◽  
Human Pathogens ◽  
Cow's Milk ◽  
Limit Of Quantitation ◽  
Time Required

Raw milk is a well-established vehicle for the carriage of human pathogens, and many regulatory bodies have consequently mandated compulsory pasteurization as a food safety intervention. The residual activity of alkaline phosphatase (ALP) has historically been used to verify the adequacy of pasteurization of cow's milk. However, there is uncertainty on how the current ALP standards and methods of analysis can be applied to sheep's and goat's milk, which naturally contain different levels of the enzyme than that found in cow's milk. The official ALP methods applied in Canada (colorimetric assay; MFO-3) and in the United States (Fluorophos) were assessed for their ability to detect enzyme activity in raw and pasteurized milk derived from cows, sheep, and goats. The detection limit and the limit of quantitation were 0.8 and 2.02 μg/ml phenol, respectively, for the MFO-3 method and 43 and 85 mU/liter, respectively, for the Fluorophos method. The average ALP levels in raw goat's, cow's, and sheep's milk were 165, 1,562, and 3,512 μg/ml phenol, respectively. Raw milk detection limits, which correspond to raw milk phosphatase levels, were 0.051, 0.485, and 0.023% in cow's, goat's, and sheep's milk, respectively, for the MFO-3 method and 0.007, 0.070, and 0.004%, respectively, for the Fluorophos method. Although both methods can be used for ALP determination in cow's, goat's, and sheep's milk, the Fluorophos assay was superior to the colorimetric MFO-3 method based on sensitivity and time required to complete the analysis.

scholarly journals Iodine content development in raw cow’s milk in three regions of the Czech Republic between the years 2008 and 2018

2019 ◽  
Vol 88 (3) ◽  
pp. 265-270
Author(s):  
Roman Konečný ◽  
Zuzana Křížová ◽  
Jan Hladký ◽  
Jitka Kautská ◽  
Lucie Hasoňová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Iodine Content ◽  
Iodine Concentration ◽  
Cow’S Milk ◽  
Human Consumption ◽  
The Czech Republic ◽  
Cow's Milk ◽  
Current State ◽  
Content Development ◽  
Raw Cow’S Milk

The study examines an analysis and evaluation of iodine content in raw cow’s milk in three regions of the Czech Republic between the years 2008 and 2018. Bulk milk samples were collected at dairy farms situated in South Bohemia, Central Bohemia, and the Vysočina Region. Iodine in milk was determined on the basis of alkaline ashing, using the spectrophotometric method according to Sandell-Kolthoff. The highest mean iodine content was measured in 2009 (485.5 ± 408.2 μg/l) and the lowest in 2016 (169.2 ± 71.7 μg/l). Since 2010 there has been a gradual decline of iodine concentration, from 479.5 ± 304.9 μg/l in 2010 to 231.2 ± 63.5 μg/l in 2018. A similar decreasing tendency was recorded in all the monitored regions. The percentage of samples containing iodine above 500 μg/l was on the decrease (37% in 2009; 0% in 2016–2018) and less than 80 μg/l (8% in 2009; 0% in 2017–2018). The current state corresponds to the requirements for iodine content in milk for human consumption. The study emphasizes the importance of continuous iodine content checks in milk and the related adjustments of iodine supplementation to the feeding rations of dairy cows.

Detection of enterotoxigenic genes inStaphylococcus aureusisolated from bulk tank cow’s milk samples in the Czech Republic

2012 ◽  
Vol 41 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Z. Šťástková ◽  
R. Karpíšková ◽  
T. Gelbíčová ◽  
V. Vaňáč ◽  
Š. Tůma ◽  
...  
Keyword(s):  
Czech Republic ◽  
Cow’S Milk ◽  
The Czech Republic ◽  
Cow's Milk ◽  
Milk Samples ◽  
Bulk Tank

scholarly journals Microbiological quality of raw milk in the Czech Republic

2016 ◽  
Vol 34 (No. 3) ◽  
pp. 189-196 ◽  
Author(s):  
Bogdanovičová Kateřina ◽  
Vyletělová-Klimešová Marcela ◽  
Babák Vladimír ◽  
Kalhotka Libor ◽  
Koláčková Ivana ◽  
...  
Keyword(s):  
Czech Republic ◽  
Cow’S Milk ◽  
Salmonella Spp ◽  
The Czech Republic ◽  
Cow's Milk ◽  
E Coli ◽  
Goat’S Milk ◽  
Goat's Milk ◽  
Campylobacter Spp

The microbiological and hygienic quality of cow’s, goat’s and sheep’s milk in the Czech Republic was evaluated. Milk (230 samples) was collected on 41 farms and investigated from May 2012 to October 2014. Milk was analysed for the presence of selected groups and types of bacteria: mesophilic microorganisms (total plate count – TPC), enterococci, Enterobacteriaceae, Escherichia coli, Staphylococcus aureus, Salmonella spp., Campylobacter spp., and Listeria monocytogenes. Besides these indicators and pathogenic agents, somatic cell count was determined as one of the indicators of mammary gland health in cows. TPC ranged between 8.3 × 10<sup>2 </sup>and 1.2 × 10<sup>9</sup> CFU/ml and somatic cells between 1.6 × 10<sup>4</sup> and 6.8 × 10<sup>6 </sup>cells/ml. The presence of E. coli was confirmed in 86.3% of samples and the colony counts ranged from 1.0 × 10<sup>1 </sup>to 4.0 × 10<sup>6 </sup>CFU/ml. The presence of verotoxigenic E. coli was confirmed in 3 samples (1.3%) (cow’s milk 0%; goat’s milk 6.3%; sheep’s milk 4.4%). The presence of S. aureus was confirmed in 29.1% of the samples (cow’s milk 26.9%; goat’s milk 34.4%; sheep’s milk 39.1%), but the numbers were very low (&lt; 5.0 × 10<sup>2</sup> CFU/ml). L. monocytogenes was confirmed in 3 examined samples (1.3%) (cow’s milk 0.6%; goat’s milk 3.1%; sheep’s milk 4.4%). Salmonella spp. and Campylobacter spp. were not detected in any of the samples tested.

“Floating Melodies and Memories” of the Terezín Memorial

Transfers ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 138-141
Author(s):  
Chia-ling Lai
Keyword(s):  
United States ◽  
Czech Republic ◽  
The United States ◽  
Concentration Camp ◽  
Dark Tourism ◽  
The Czech Republic ◽  
Will To Live ◽  
Worldwide Phenomenon ◽  
The Will ◽  
Musical Heritage

As Andrea Huyssen observes, since the 1990s the preservation of Holocaust heritage has become a worldwide phenomenon, and this “difficult heritage” has also led to the rise of “dark tourism.” Neither as sensationally traumatic as Auschwitz’s termination concentration camp in Poland nor as aesthetic as the forms of many modern Jewish museums in Germany and the United States, the Terezín Memorial in the Czech Republic provides a different way to present memorials of atrocity: it juxtaposes the original deadly site with the musical heritage that shows the will to live.

Evaluation of Spectrophotometric and Fluorometric Methods for Alkaline Phosphatase Activity Determination in Ewe's Milk

2000 ◽  
Vol 63 (9) ◽  
pp. 1258-1261 ◽  
Author(s):  
M. F. SCINTU ◽  
E. DAGA ◽  
A. LEDDA
Keyword(s):  
Alkaline Phosphatase ◽  
Standard Deviation ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Official Method ◽  
Cow's Milk ◽  
Alp Activity ◽  
Relative Standard ◽  
Ewe's Milk ◽  
European Method

The alkaline phosphatase (ALP) activity test has been used since 1935 to assess the effectiveness of pasteurization. Different analytical methods exist for detecting ALP in milk. Unfortunately, there is little information about ALP activity in ewe's milk. The aim of this study was to assess and compare the official European method (spectrophotometric method) and the Fluorophos method (fluorometric method) regarding their use in ewe's milk. Bulk ewe's milk samples were taken from a flock and from three different dairies. A portion of the original sample was pasteurized at 63°C for 30 min in a circulating bath; another portion was heated to and kept at 95°C for about 2 min, and 0.1% (vol/vol) of raw milk was added. The samples obtained were analyzed in duplicate using the spectrophotometric and fluorometric methods. The relation between ALP activity determined by the two methods was characterized by the following equation: Y = 1.34 + 0.0039X (where Y = ALP in μg of phenol per ml of milk and X = ALP in mU/liter; R2 = 91.5%). Precision parameters (repeatability [r], standard deviation of repeatability [sr], and relative standard deviation of repeatability [RSDr]) for both methods were calculated. The values of RSDr for the Fluorophos method were 4.30 for pasteurized milk and 2.96 for 0.1% raw milk, close to the value indicated by Rocco in whole cow's milk (RSDr = 4.4). The repeatability for the official method (r = 2.16) was close to that indicated for whole cow's milk (r = 2).

The Use of Whole Cow's Milk in Infancy

PEDIATRICS ◽  
1992 ◽  
Vol 89 (6) ◽  
pp. 1105-1109 ◽  
Author(s):  
Keyword(s):  
Breast Milk ◽  
American Academy ◽  
Infant Formula ◽  
Iron Status ◽  
The United States ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Solid Foods ◽  
American Academy Of Pediatrics ◽  
Iron Deficient

The pediatrician is faced with a difficult challenge in providing recommendations for optimal nutrition in older infants. Because the milk (or formula) portion of the diet represents 35% to 100% of total daily calories and because WCM and breast milk or infant formula differ markedly in composition, the selection of a milk or formula has a great impact on nutrient intake. Infants fed WCM have low intakes of iron, linoleic acid, and vitamin E, and excessive intakes of sodium, potassium, and protein, illustrating the poor nutritional compatibility of solid foods and WCM. These nutrient intakes are not optimal and may result in altered nutritional status, with the most dramatic effect on iron status. Infants fed iron-fortified formula or breast milk for the first 12 months of life generally maintain normal iron status. No studies have concluded that the introduction of WCM into the diet at 6 months of age produces adequate iron status in later infancy; however, recent studies have demonstrated that iron status is significantly impaired when WCM is introduced into the diet of 6-month-old infants. Data from studies abroad of highly iron-deficient infant populations suggest that infants fed partially modified milk formulas with supplemental iron in a highly bioavailable form (ferrous sulfate) may maintain adequate iron status. However, these studies do not address the overall nutritional adequacy of the infant's diet. Such formulas have not been studied in the United States. Optimal nutrition of the infant involves selecting the appropriate milk source and eventually introducing infant solid foods. To achieve this goal, the American Academy of Pediatrics recommends that infants be fed breast milk for the first 6 to 12 months. The only acceptable alternative to breast milk is iron-fortified infant formula. Appropriate solid foods should be added between the ages of 4 and 6 months. Consumption of breast milk or iron-fortified formula, along with age-appropriate solid foods and juices, during the first 12 months of life allows for more balanced nutrition. The American Academy of Pediatrics recommends that whole cow's milk and low-iron formulas not be used during the first year of life.

scholarly journals Evaluation of the Physicochemical Quality of Raw Bovine and Goat Milk Marketed in the Steppic Region of Djelfa (Algeria)

2019 ◽  
Vol 76 (1) ◽  
pp. 55
Author(s):  
Mourad HAMIROUNE ◽  
Sounia DAHMANI ◽  
Zineb KASMI ◽  
Abdelhamid FOUGHALIA ◽  
Mahmoud DJEMAL
Keyword(s):  
Freezing Point ◽  
Bovine Milk ◽  
Goat Milk ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Physicochemical Quality ◽  
Cow's Milk ◽  
Goat’S Milk ◽  
Disciplinary Procedures ◽  
Goat's Milk

This research was conducted to study the key physicochemical parameters of samples of raw bovine and goat milk collected in the steppic region of Djelfa. One hundred and six samples of raw milk were collected from April 2018 to May 2018, at points of sale and analyzed. The results showed that cow’s milk had 3.66±0.89% fat, 11.4±1.56% solid not fat, 4.35±0.61% protein, 6.35±0.89% lactose and a density of 1.0360±0.0056 with a freezing point of -0.380±0.053 °C. While goat’s milk had 3.43±0.65% fat, 10.2±0.92% solid not fat, 3.88±0.36% protein, 5.66±0.52% lactose and a density of 1.0317±0.0035 with a freezing point of -0.348±0.044 °C. This proves that cow’s milk has a slightly higher physicochemical quality than goat’s milk. In addition, the present study showed that 100% raw goat milk is wet against 97.1% raw bovine milk. This indicates the presence of cases of fraud requiring disciplinary procedures. Moreover, in the majority of the cases, the storage temperatures of the milk far exceed the values recommended by the Algerian standards (+6°C). It is necessary to establish a program of control and popularization of all the actors of the sector in order to improve the quality and the quantity of raw milk produced.

scholarly journals Analysis of development of basic characteristics of the dairy industry in the Czech Republic

2005 ◽  
Vol 53 (6) ◽  
pp. 93-100
Author(s):  
Renata Kučerová
Keyword(s):  
Growth Rate ◽  
Czech Republic ◽  
Life Cycle ◽  
Dairy Industry ◽  
Raw Milk ◽  
The European Union ◽  
Agricultural Producers ◽  
The Czech Republic ◽  
Market Growth ◽  
Basic Characteristics

The paper deals with the analysis of changes in the development of basic characteristics of the dairy industry in the Czech Republic, which cohere with the integration of the Czech Republic into the European Union. The attention is paid on size of the market, growth rate, life cycle, development of prices and development of foreign trade. The total domestic consumption reached 2111.1 million litres in 2004. The industry is in the maturity. The excess of supply exists in the industry; the growth rate is low, under 5% per year. The integration of the Czech Republic into the EU didn’t bring about changes in the development of basic characteristics – size of the market, growth rate, and life cycle. The volume of production changed. The total volume of purchase of raw milk for production went down by 1.4% to the value in 2000. And all prices in the product vertical – milk and milk products (prices of agricultural producers, production prices and consumer’s prices) rose.The paper is a part of solution of the research plan of the FBE MUAF in Brno, No. MSM 6215648904.

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