scholarly journals Immunophenotyping of Peripheral Blood Lymphocytes in Saudi Men

2002 ◽  
Vol 9 (2) ◽  
pp. 279-281 ◽  
Author(s):  
Abdulla Al Qouzi ◽  
Abdulla Al Salamah ◽  
Reem Al Rasheed ◽  
Abdulla Al Musalam ◽  
Khalid Al Khairy ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
B Cells ◽  
Adult Population ◽  
Cell Subset ◽  
Peripheral Blood Lymphocytes ◽  
Published Data ◽  
Reference Ranges ◽  
Cd4 Cells ◽  
Cd8 Cells ◽  
The Absolute

ABSTRACT Flow cytometry is an important tool for the diagnosis and follow-up of immunodeficiency patients, as well as for pateints with leukemia and lymphoma. Lymphocytes and their subsets show variations with race. The aim of this study was to establish reference ranges for lymphocytes and their subsets in an Saudi adult population by using flow cytometry. Blood samples obtained from 209 healthy Saudi men were used for this study. All blood donors were between 18 and 44 years old. Lymphocytes and their subsets were analyzed by flow cytometry, and the absolute and percentage values were calculated. We investigated the expression of T-cell markers (CD3, CD4, and CD8), B cells (CD19), and natural killer cells (CD16 and CD56). The absolute and percent values of each cell subset were compared with published data from different populations by using the Student t test. Reference ranges, each expressed as the mean ± the standard deviation, were as follows: leukocytes (6,335 ± 1759), total lymphocytes (2,224 ± 717), CD3 cells (1,618 ± 547), CD4 cells (869 ± 310), CD8 cells (615 ± 278), CD19 cells (230 ± 130), and CD3-CD16+/CD56+ cells (262 ± 178). The CD4/CD8 ratio was 1.6 ± 0.7. Our results for B cells, CD4 cells, and CD8 cells and for the CD4/CD8 ratio fell in between the reported results for Ethiopian and Dutch subjects. Our results were also different from previously reported findings in an Saudi adult population that showed no increase in CD8 T cells. We thus establish here the reference ranges for lymphocytes and their subsets in a large cohort of Saudi men. The CD8 cell count was not abnormally high, as previously reported, and fell in between previous results obtained for African and European populations.

scholarly journals OP0186 CHANGES IN CIRCULATING B CELL LEVELS AND IMMUNOPHENOTYPE ARE ASSOCIATED WITH DEVELOPMENT OF ARTHRITIS FOLLOWING TREATMENT WITH CHECKPOINT INHIBITORS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 112.2-113
Author(s):  
M. Gatto ◽  
S. Bjursten ◽  
C. Jonell ◽  
C. Jonsson ◽  
S. Mcgrath ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
B Cells ◽  
Adverse Events ◽  
B Cell ◽  
Mononuclear Cells ◽  
Checkpoint Inhibitors ◽  
Therapy Monitoring ◽  
Cell Subset ◽  
Peripheral Blood Mononuclear ◽  
Transitional B Cells

Background:Inflammatory arthritis (IA) is frequent among rheumatic side effects induced by checkpoint inhibitor (CPI) therapy for metastatic malignancies1. While T cells are likely to sustain the inflammatory process2, fewer data are available concerning the role of B cells3.Objectives:To investigate the phenotype of circulating B cells in patients who develop CPI-induced IA (CPI-IA) and to compare it with features of B cells in patients not developing immune-related adverse events (irAE) upon CPI treatment.Methods:B cell subsets at baseline (before CPI initiation) and during CPI treatment were analyzed in CPI-IA patients and in patients receiving CPI but who did not develop irAE (non-irAE). Peripheral blood mononuclear cells (PBMC) were analyzed by flow cytometry and B cells were identified as CD19+ and divided into naïve (CD27-IgD+), memory (CD27+IgD+/-), double negative (CD27-IgD-) and transitional (CD10+CD24+CD38+/hi) B cells. Levels of CD21, an activation marker on transitional B cells, were also analyzed. Non-parametric tests were used for analysis of differences between groups.Results:Six CPI-IA and 7 non-irAE patients matched for age, gender and CPI treatment were included, who had received CPI treatment due to metastatic melanoma. Flow cytometry revealed a significant increase of circulating B cells (p=0.002) (Figure 1A) and especially of transitional B cells in CPI-IA patients vs. non-irAE (median %, range: 7.8 (4.5-11.4) vs. 3.2 (1.6-4.3),p=0.007) (Figure 1B), while no remarkable changes were seen across other subsets. Transitional B cell levels significantly decreased from active to quiescent CPI-IA in all patients (p=0.008). In two CPI-IA patients for whom baseline sampling was available, the increase of transitional levels occurred early after CPI treatment and before CPI-IA onset. Levels of expression of CD21 on transitional B cells were increased in CPI-IA vs. non-irAE (p=0.01).Conclusion:Transitional B cells are expanded in CPI-IA patients and seem to increase early after start of CPI therapy. Monitoring this B cell subset might lead to closer follow-up and earlier diagnosis of CPI-IA.References:[1]Ramos-Casals M, Brahmer JR, Callahan MK, et al. Immune-related adverse events of checkpoint inhibitors. Nat Rev Dis Primers 2020;6:38[2]Murray-Brown W, Wilsdon TD, Weedon H, et al. Nivolumab-induced synovitis is characterized by florid T cell infiltration and rapid resolution with synovial biopsy-guided therapy. J Immunother Cancer 2020;8:e000281[3]Das R, Bar N, Ferreira M, et al. Early B cell changes predict autoimmunity following combination immune checkpoint blockade. J Clin Invest. 2018;128:715-2Disclosure of Interests:None declared

scholarly journals Effects of classical swine fever virus infection on the porcine leukocyte subsets

2000 ◽  
Vol 48 (1) ◽  
pp. 35-42 ◽  
Author(s):  
H. J. Schubert ◽  
P. Soós ◽  
K. R. Depner
Keyword(s):  
Flow Cytometry ◽  
Virus Infection ◽  
Viral Antigen ◽  
Classical Swine Fever ◽  
Virus Antigen ◽  
Lower Percentage ◽  
Cd4 Cells ◽  
Double Positive ◽  
Cd8 Cells ◽  
Acute Form

The effects of classical swine fever (CSF) virus infection on the porcine leukocyte subsets were investigated by flow cytometry in acute, chronic and convalescent forms of the disease. The virus antigen could be first detected in the monocytes on postinfection (p.i.) day 10 while in the lymphocytes on p.i. day 13. It could be established that the ratio of CD6+ cells decreased until p.i. day 6, but afterwards it started to increase and reached different values. The CD4+CD8+, the CD8+ and the CD6- cells were obviously higher virus positive than the CD4+ and the CD4-CD8-subsets, but essentially all subsets could be infected. The ratio of CD8+ cells increased during the disease, while the number of double positive cells decreased, and that of the CD4+ cells was variable. The viral antigen could be detected in a lower percentage of the CD4+CD8+, CD8+, CD6+ and CD6- cells of the pigs affected with the chronic form of the disease than in those with the acute form. During the experiments no viral antigen could be detected in the leukocytes of the pig that became convalescent, though the changes in its leukocyte subsets were very similar to those seen in pigs in which the viral antigen could be detected. The studies have revealed that essentially all leukocyte subsets can be infected with the CSF virus, but in very different amounts.

Islet Allograft Rejection in Rats: a Time Course Study Characterizing Adhesion Molecule Expression, Mhc Expression, and Infiltrate Immunophenotypes

1998 ◽  
Vol 7 (3) ◽  
pp. 285-297 ◽  
Author(s):  
Douglas A. Coddington ◽  
Hua Yang ◽  
Geoffrey Rowden ◽  
Pat Colp ◽  
Thomas B. Issekutz ◽  
...  
Keyword(s):  
B Cells ◽  
Allograft Rejection ◽  
Time Course ◽  
Class Ii ◽  
Cd4 Cells ◽  
Islet Allograft ◽  
Cd8 Cells ◽  
Class Ii Mhc ◽  
Infiltrating Cells ◽  
Parenchymal Cells

Wistar Furth (RT1u) islets transplanted under the renal capsules of streptozotocin-diabetic Lewis (RT1l) rats reject after 5–6 days of normoglycemia. Hand-picked WF islets (1500–2000) were transplanted under the kidney capsules of diabetic Lew or WF rats. Rats bearing iso- or allografts were killed on posttransplant days 2, 4, and 6. Serial frozen sections of grafts and controls were stained by immunoperoxidase for rat MAC-1, class II MHC, CD2, CD4, CD8, B-cells, VLA-4, LFA-1, L-selectin, ICAM-1, and VCAM-1. Infiltrating cells, parenchymal cells, and endothelial cells in five distinct compartments (i.e., peritoneal reflection, subcapsular perivascular space, islet grafts, graft–kidney interface, and kidney) were evaluated for expression of the various markers at each interval. Significant infiltrates arrived in three distinct waves in both iso- and allografts. First, macrophages blanketed the peritoneal capsular reflection and infiltrated by day 2. Second, the first wave of lymphocytes arrived in the edematous subcapsular soft tissue via capsular vessels by day 2 (allo > iso). Third, the second wave of lymphocytes arrived from the renal parenchyma to form a dense band at the graft–kidney interface and around grafts by days 4 and 6 (allo >>> iso); CD4+ cells vastly outnumbered CD8+ cells, with CD4+ cells being mobilized first and from interstitial vessels throughout the entire kidney. CD8+ cells emigrated only from renal interstitial vessels adjacent to the graft. Large numbers of L-selectin+, VLA-4+, and LFA-1+ cells were seen in the infiltrates with the most intensely staining cells being intravascular. B-cells composed a very small proportion of infiltrating cells in both allo- and isografts. Endothelial staining for ICAM-1 and VCAM-1 was prominent throughout. Both class II MHC and ICAM-1 expression were induced on renal tubular epithelial cells, but neither was found on islet parenchymal cells. In conclusion, this study shows that islet allograft rejection is more complex than previously realized.

Treatment and biology of lymphomatoid granulomatosis

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 8029-8029 ◽  
Author(s):  
B. R. Healey Bird ◽  
N. Grant ◽  
K. Dunleavy ◽  
J. Janik ◽  
J. Cohen ◽  
...  
Keyword(s):  
B Cells ◽  
Lymphocyte Subsets ◽  
High Dose ◽  
Cd4 Cells ◽  
Cd8 Cells ◽  
Viral Loads ◽  
The Mean ◽  
Male Sex ◽  
Age Range ◽  
Grade Iii

8029 Background: LYG is a rare angiocentric-destructive process with EBV+ B-cells and reactive T-cells. LYG is graded with grades I-II showing rare-moderate large EBV+ B-cells (usually polyclonal or oligoclonal) and grade III showing numerous large EBV+ B-cells (usually monoclonal), likely reflecting progressive transformation. Historically, steroids and/or chemotherapy have a 14 mos median survival. Methods: We are investigating Interferon-a (I-a) for grade I/II and dose-adjusted EPOCH ±Rituximab (R) for grade III LYG. Results: Characteristics of 53 pts are: male sex 68%; median age (range) 46 (17–67) and median ECOG P.S. 1 (0–3). Disease sites include lung 98%, CNS 38%, kidney 15%, skin 17%, liver 19% and nodes 4%. On study LYG grades are I-30%, II-26% and III-44%. Prior treatment was none-28%, chemotherapy± R-34%, and steroids alone-40% of pts. For grades I/II, I-a is begun at 7.5 million IUs TIW and escalated as tolerated until disease regression and continued 1 yr after CR. Of 31 patients treated with I-a, PFS is 62% at the median f/u of 5.3 yrs. Of 25 evaluable pts (3 NE; 3 TE), 60% had sustained CR for a median of 60 mos (4–175). In 9 pts who progressed on I-a, grade III was found in 5. Thus, in 20 pts with only grade I/II, 75% had sustained CR with I-a. In 11 evaluable pts with CNS disease, 81% achieved remission with I-a alone. The median time to remission is 9 mos (3–40) and median I-a dose is 20 MIU (7–40). Among 24 pts receiving DA-EPOCH±R, PFS is 40% at the median f/u of 28 mos. Of 21 evaluable pts (2 NE, 1 TE), 66% achieved CR. OS of all 53 pts is 68% at the median f/u of 4 yrs. Median EBV viral loads in 29 pts at study entry were 18 copies/10e6 genome equivalents (0–22727) (normal<200). Lymphocyte subsets in 30 pts showed a median CD4–428 (24–2322) and CD8–165 cells/mm3 (42–1316). In 12 pts in CR and with serial values, the mean CD8 cells (131 ± 44) (p2= 0.013) but not CD4 cells (65 ± 75) increased with treatment. Conclusions: High dose I-a produces sustained remissions in grade I/II LYG and is effective in CNS LYG. DA-EPOCH±R can produce durable CRs in grade III LYG. We hypothesize LYG emerges in a compromised immune milieu and undergoes progressive transformation if not effectively treated. Historical results suggest steroids may allow transformation by compromising immune function. No significant financial relationships to disclose.

Influence of Race, Age and Sex on the Lymphocyte Subsets in Peripheral Blood of Healthy Malaysian Adults

1995 ◽  
Vol 32 (6) ◽  
pp. 532-539 ◽  
Author(s):  
M L Choong ◽  
S H Ton ◽  
S K Cheong
Keyword(s):  
T Cells ◽  
B Cells ◽  
Nk Cells ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Reference Range ◽  
Suppressor Cells ◽  
Skewed Distribution ◽  
Cd4 Cells ◽  
Cd8 Cells

The lymphocyte subsets in the peripheral blood of healthy Malaysian adults (212 subjects, age 18–71 years) were analysed using a flow cytometer FACScan in an effort to establish a reference range for the lymphocyte subsets. The lymphocyte subsets studied were T cells (CD3), B cells (CD19), natural killer (NK) cells (CD3−CD16+/CD56+), helper/inducer cells (CD4), cytotoxic/suppressor cells (CD8) and the helper/suppressor ratio (CD4/CD8). The distributions of T cells, CD4 cells and CD8 cells were symmetric about their means while B cells, NK cells and CD4/CD8 ratio followed a skewed distribution. Differences in race were observed for T cells, NK cells, CD4 cells and CD4/CD8 ratio where the Indians were significantly different from the Malays and the Chinese (higher T cells, CD4 cells and CD4/CD8 ratio and lower NK cells). The B cells were significantly lower in the Chinese than the Malays and the Indians. Age differences were seen only in the Chinese where increased CD4 cells and CD4/CD8 ratio, and decreased CD8 cells were observed. A sex difference was observed only in the Chinese where the CD4/CD8 ratio was significantly higher in females than males.

Changes in T-cell subpopulations in mice during prolonged experimental secondary infection with Echinococcus granulosus

1995 ◽  
Vol 15 (4) ◽  
pp. 201-208 ◽  
Author(s):  
M. C. Rueda ◽  
A. Osuna ◽  
P. H. De Rycke ◽  
D. Janssen
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Echinococcus Granulosus ◽  
Peripheral Blood ◽  
Secondary Infection ◽  
Interleukin 2 ◽  
Cd4 Cells ◽  
Cd8 Cells ◽  
Cell Subpopulations ◽  
T Cell Subpopulations

Balb/c mice were infected intraperitoneally with protoscoleces of Echinococcus granulosus. After 15 months of infection, and by means of flow cytometry, the expression of T-cell markers CD3, CD4, and CDS on T cells from peripheral blood, spleen, and thymus was analyzed and compared with that of age-matched controls. Infected mice had higher percentages of CD3+, and CD4+ cells in peripheral blood, and higher percentages of CD8+ cells in the spleen, when compared with control mice. CD4+ and CD8+ cells in peripheral blood and CD8+ cells in thymus also showed higher percentages of expression of interleukin-2 receptor. The results infer a role for interleukin-2 in experimental secondary echinococcosis.

scholarly journals Establishment of Adult Peripheral Blood Lymphocyte Subset Reference Range for an Asian Population by Single-Platform Flow Cytometry: Influence of Age, Sex, and Race and Comparison with Other Published Studies

2004 ◽  
Vol 11 (1) ◽  
pp. 168-173 ◽  
Author(s):  
Wee J. Chng ◽  
Guat B. Tan ◽  
Ponnudurai Kuperan
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood Lymphocyte ◽  
Peripheral Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Subsets ◽  
Reference Range ◽  
Nk Cell ◽  
Published Data ◽  
Reference Ranges ◽  
Cell Counts

ABSTRACT We established a normal reference range for peripheral blood lymphocyte subsets in a multiracial adult population by using single-platform flow cytometry. Further analysis of our cohort showed that the CD8+-cell counts decrease with age, there is a gender difference in NK cell percentages and counts, and there are significant differences in the CD3+-, CD4+-, and CD19+-cell counts between Indians and other racial groups. Overall, our results are significantly different from other published data. This difference further stresses the need for different populations to establish their own reference ranges as these may have important implications for the management of patients with human immunodeficiency virus and AIDS. The use of single-platform flow cytometry will eliminate some of the variability between different study centers, making studies more comparable. This platform should be used for future studies into the effects of age, sex, and race on lymphocyte subsets.

scholarly journals Effect of Diet and Exercise on the Peripheral Immune System in Young Balb/c Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
B. E. Martínez-Carrillo ◽  
R. A. Jarillo-Luna ◽  
R. Campos-Rodríguez ◽  
R. Valdés-Ramos ◽  
V. Rivera-Aguilar
Keyword(s):  
Oxidative Stress ◽  
Immune System ◽  
B Cells ◽  
Lymphocyte Subpopulations ◽  
Cd4 Cells ◽  
Cd8 Cells ◽  
Peripheral Immune System ◽  
Diet And Exercise ◽  
Unbalanced Diet ◽  
And Oxidative Stress

Although diet and exercise clearly have an influence on immune function, studies are scarce on the effect caused by exercise and the consumption of a carbohydrate-rich or fat-rich diet on the peripheral immune system. The aim of the present study was to evaluate the effect of exercise and the two aforementioned unbalanced diets on young Balb/c mice, especially in relation to BMI, the level of glucose, and the percentage of lymphocyte subpopulations in peripheral blood. The changes found were then related to the synthesis of leptin and adiponectin as well as the production of oxidative stress. The increase in BMI found with the carbohydrate-rich and fat-rich diets showed correlation with the levels of leptin and adiponectin. An increase in leptin and a decrease in adiponectin directly correlated with an increase in total lymphocytes and CD4+ cells and with a decrease in B cells. The increase in leptin also correlated with an increase in CD8+ cells. Glycemia and oxidative stress increased with the two unbalanced diets, negatively affecting the proliferation of total lymphocytes and the percentage of B cells, apparently by causing alterations in proteins through carbonylation. These alterations caused by an unbalanced diet were not modified by moderate exercise.

Peripheral Blood Lymphocytes Subpopulations and Apoptotic Markers in Patients with Lymphoid Malignancies and in Controls: An Epidemiologic Case-Control Study.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3858-3858
Author(s):  
Marc M. Maynadie ◽  
Romain Casey ◽  
Karine Piazzon ◽  
Jean Claude Capiod ◽  
Paule-Marie Carli
Keyword(s):  
B Cells ◽  
B Cell ◽  
Peripheral Blood ◽  
Case Control Study ◽  
Cell Subset ◽  
Case Control ◽  
Peripheral Blood Lymphocytes ◽  
Lymphoid Malignancies ◽  
Proliferation And Apoptosis ◽  
Control Study

Abstract Few references ranges of normal peripheral blood lymphocytes subpopulations are available in the literature and fewer data were available regarding activation, proliferation and apoptosis antigen expression on such populations. We studied these parameters in patients included in an epidemiologic case-control study on risk factors of lymphoid malignancies conducted within European countries. Cell surface staining of peripheral blood lymphocyte antigens were analysed by multicolour flow cytometry in 300 cases and 300 controls. We determined CD3+, CD3+/CD4+, CD3+/CD8+, CD3−/CD56+CD16+, CD19+, CD19+/CD5+, CD19+/CD5− and CD57+ populations. Expression of CD25, CD16, CD40, CD154, CD95 and CD178 were studied on these populations. CD expressions were compared by multiple regressions between controls and diseases, after stratification on circulating phase. In controls we observed a significant decrease of B cells and an increase of NK cells with age. No difference was found according to sex, smoking status and Body Mass Index. In Follicular Lymphoma, Diffuse Large B Cell Lymphoma and Marginal Zone Lymphoma (MZL) without circulating phase cases, we observed a decrease of the B cell subset and an increase of the NK cell subset instead of only a trend was found in Multiple Myeloma, Mycosis Fongoides, Hodgkin Disease and Lymphoplasmacytic Lymphoma. CD95 expression was increased in HD, DLBCL, MZL without circulating phase and Hairy Cell Leukemia but decreased in B-cell Chronic Lymphocytic Leukemia and MZL with circulating phase. CD40 was decreased on B cells in HD, FL, MZL, B-ALL and CLL. This study was one of the most important, in term of number of patients included, particularly concerning data on activation, proliferation and apoptosis markers in normal subjects but also in several lymphoid malignancies.

scholarly journals Unswitched Memory B Cell Deficiency Is Associated with Acute Chest Syndrome and Stroke in Sickle Cell Disease

Blood ◽  
2019 ◽  
Vol 134 (Supplement_1) ◽  
pp. 1010-1010
Author(s):  
Sameera Bhamidipati ◽  
Venee N. Tubman ◽  
Norma Estrada ◽  
Charles Minard
Keyword(s):  
Flow Cytometry ◽  
B Cells ◽  
Sickle Cell Disease ◽  
Blood Transfusion ◽  
B Cell ◽  
Cell Subset ◽  
Acute Chest Syndrome ◽  
Cell Disease ◽  
History Of

Background: The spleen is among the first organs negatively affected by sickle cell disease (SCD). Unswitched memory B cells (UMBCs) from the spleen, including the marginal zone B cells, circulate in peripheral blood and have been used as a measure of splenic function in non-hematologic disorders associated with functional asplenia. UMBC deficiency has been associated with adverse infectious and inflammatory outcomes in models of stroke and cardiovascular disease. The role of UMBCs has not been assessed in pediatric SCD. In this study, we sought to test he hypothesis that low UMBC is associated with significant infectious and inflammatory complications of SCD. Design/Method: Children seen at the Texas Children's Cancer and Hematology Centers from March - December 2018 were recruited for participation. Participants were 18 months -18 years of age. Samples were collected for complete blood count, serum analysis, and B cell subset by flow cytometry. For B cell subset quantification, whole blood samples were stained with fluorescent-labelled CD45, CD19, IgD, and CD27. The samples were analyzed by flow cytometry using the BD LSR Fortessa or LSR II (BD Biosciences, USA). In each sample, at least 2500 CD19+ events were captured. Data were analyzed using FlowJo X.V. Clinical data was abstracted from the electronic medical record into an online study database. Statistical analyses were performed using STATA. This study was approved by the Baylor College of Medicine IRB. Results: We enrolled 234 subjects. Among these, 169 had an evaluable UMBC. Those with UMBC data included 114 (67.4%) Hb SS and Hb S/b0-thalassemia, 14 (8.3%) Hb SC, Hb b+-thalassemia, and HbS/other, and 41 (24.2%) controls without SCD. There was no difference in the mean age at sampling between children with SCD and controls without SCD (8.74 vs 8.39 years, P=0.70). The geometric mean UMBC was 5.75% of all CD19+ (B) cells for controls, 4.24 % for HbSS-like group, and 5.29% for SCD patients with any other genotype. UMBC declined by 2.97% per year among all SCD subjects (P<0.01). UMBC declined 0.82% per year among all controls, although the rate of decline was not significant, P= 0.62). The change per year did not significantly differ between cohorts (P=0.26). To determine whether UMBC is associated with clinical complications of SCD, we reviewed the EMR of 127 subjects to identify any lifetime episodes of acute chest syndrome, bacteremia, blood transfusion, dactylitis, or stroke. Patients with any history of acute chest syndrome had significantly lower UMBC than those who have not had acute chest syndrome (median 4.3 vs. 6.2; P< 0.01). Patients with any history of stroke had significantly lower UMBC than those who have not had stroke (median 2.9 vs. 4.7; P=0.04). No significant difference was detected between groups with and without a history of bacteremia, blood transfusion, or dactylitis. Conclusion: We have demonstrated that children with SCD are deficient in UMBCs and that UMBC deficiency worsens with age. UMBC deficiency is associated with history of acute chest syndrome and stroke. Additional studies are needed to establish the mechanisms of UMBC deficiency and the role of UMBCs in the clinical outcomes for children with SCD. Table Disclosures Tubman: Novartis Pharmceuticals: Honoraria.

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