RESPIRATION AND PHOSPHORUS-CONTAINING COMPOUNDS IN NORMAL AND TUMOR TISSUES OF RED BEET ROOTS

1962 ◽  
Vol 40 (9) ◽  
pp. 1251-1256 ◽  
Author(s):  
K. J. Scott ◽  
R. M. Smillie ◽  
G. Krotkov
Keyword(s):  
Inorganic Phosphate ◽  
Analytical Study ◽  
The Other ◽  
Cell Numbers ◽  
Normal Tissues ◽  
Red Beet ◽  
Tumor Tissues ◽  
Labile Phosphorus ◽  
Phosphorus Containing ◽  
Acid Labile

A comparative analytical study has been made of normal and tumor tissues of red beet roots. Per μg DNA, the two tissues had similar cell numbers, protein contents, and respiratory rates. On the other hand, the fresh and dry weights, acid-soluble phosphorus including nucleotides, acid-labile phosphorus, and inorganic phosphate were higher in the normal tissues. Tumor tissues contained more RNA. These results are discussed in relation to the cellular metabolism of the normal and tumor tissues and it is suggested that the main metabolic differences between these tissues lie in the utilization of respiratory energy, rather than in its production.

scholarly journals Changes in Nucleic Acids and Other Phosphorus-Containing Compounds of Developing Wheat Grain

1963 ◽  
Vol 16 (2) ◽  
pp. 332 ◽  
Author(s):  
AC Jennings ◽  
RK Morton
Keyword(s):  
Phytic Acid ◽  
Organic Phosphorus ◽  
Inorganic Phosphorus ◽  
Rapid Decline ◽  
Labile Phosphorus ◽  
Phosphorus Containing ◽  
Soluble Acid ◽  
Phosphoryl Groups ◽  
The Times ◽  
Acid Labile

In both tho developing endosperm and testa-perica.rp thero was a relationship between the content of inorganic phosphorus, acid-soluble acid-labile phosphorus, and acid-soluble organic phosphorus ("phytic acid"), and the water content. The times of restriction of supply of water to the endosperm and to the testa-perion!'p fairly closely corresponded with the times of initiation of rapid synthei?-is of phytic acid and of a corresponding rapid decline in content of inorganic phosphorus and reactive phosphoryl groups.

Calreticulin is Differentially Expressed in Invasive Ductal Carcinoma: A Comparative Study

2019 ◽  
Vol 16 (2) ◽  
pp. 148-155
Author(s):  
Asma Tariq ◽  
Rana Muhammad Mateen ◽  
Iram Fatima ◽  
Muhammad Waheed Akhtar
Keyword(s):  
Invasive Ductal Carcinoma ◽  
Tumor Tissue ◽  
Ductal Carcinoma ◽  
Tissue Level ◽  
Differentially Expressed ◽  
Breast Cancer Patients ◽  
Two Dimensional Gel Electrophoresis ◽  
Normal Tissues ◽  
Tumor Tissues ◽  
Grade Ii

Objective: The aim of the present study was to build protein profiles of untreated breast cancer patients of invasive ductal carcinoma grade II at tissue level in Pakistani population and to compare 2-D profiles of breast tumor tissues with matched normal tissues in order to evaluate for variations of proteins among them. Materials & Methods: Breast tissue profiles were made after polytron tissue lysis and rehydrated proteins were further characterized by using two-dimensional gel electrophoresis. On the basis of isoelectric point (pI) and molecular weight, proteins were identified by online tool named Siena 2-D database and their identification was further confirmed by using MALDI-TOF. Results: Among identified spots, 10 proteins were found to be differentially expressed i.e.; COX5A, THIO, TCTP, HPT, SODC, PPIA, calreticulin (CRT), HBB, albumin and serotransferrin. For further investigation, CRT was selected. The level of CRT in tumors was found to be significantly higher than in normal group (p < 0.05). The increased expression of CRT level in tumor was statistically significant (p = 0.010) at a 95% confidence level (p < 0.05) as analyzed by Mann-Whitney. CRT was found distinctly expressed in high amount in tumor tissue as compared to their matched normal tissues. Conclusion: It has been concluded that CRT expression could discriminate between normal tissue and tumor tissue so it might serve as a possible candidate for future studies in cancer diagnostic markers.

Polymer-Graphene Nanoassemblies and their Applications in Cancer Theranostics

2020 ◽  
Vol 20 (11) ◽  
pp. 1340-1351 ◽  
Author(s):  
Ponnurengam M. Sivakumar ◽  
Matin Islami ◽  
Ali Zarrabi ◽  
Arezoo Khosravi ◽  
Shohreh Peimanfard
Keyword(s):  
Mechanical Stability ◽  
Chemical Properties ◽  
The Other ◽  
Hydrophilic Polymer ◽  
Two Dimensional ◽  
Normal Tissues ◽  
Physical Chemical ◽  
Anti Cancer ◽  
Good Biocompatibility ◽  
Cancer Theranostics

Background and objective: Graphene-based nanomaterials have received increasing attention due to their unique physical-chemical properties including two-dimensional planar structure, large surface area, chemical and mechanical stability, superconductivity and good biocompatibility. On the other hand, graphene-based nanomaterials have been explored as theranostics agents, the combination of therapeutics and diagnostics. In recent years, grafting hydrophilic polymer moieties have been introduced as an efficient approach to improve the properties of graphene-based nanomaterials and obtain new nanoassemblies for cancer therapy. Methods and results: This review would illustrate biodistribution, cellular uptake and toxicity of polymergraphene nanoassemblies and summarize part of successes achieved in cancer treatment using such nanoassemblies. Conclusion: The observations showed successful targeting functionality of the polymer-GO conjugations and demonstrated a reduction of the side effects of anti-cancer drugs for normal tissues.

The RAV12 Monoclonal Antibody Recognizes the N-Linked Glycotope RAAG12: Expression in Human Normal and Tumor Tissues

2009 ◽  
Vol 133 (9) ◽  
pp. 1403-1412
Author(s):  
Suzanne K. Coberly ◽  
Francine Z. Chen ◽  
Mark P. Armanini ◽  
Yan Chen ◽  
Peter F. Young ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antigen Expression ◽  
Subcellular Location ◽  
Carbohydrate Antigen ◽  
Cytoplasmic Staining ◽  
Safety Issues ◽  
Normal Tissues ◽  
Tumor Tissues ◽  
Associated Proteins

Abstract Context.—RAAG12 is a primate-restricted N-linked carbohydrate antigen present on multiple membrane-associated proteins. RAAG12 is recognized by the RAV12 monoclonal antibody. RAV12 binds to RAAG12-expressing gastrointestinal adenocarcinomas, modifies growth factor-mediated signaling, induces oncotic cell death in vitro, and has antitumor activity toward gastrointestinal tumor xenografts. Objective.—To determine the expression pattern of RAAG12 in normal and tumor tissue to identify indications for clinical study and potential safety issues. Design.—Immunohistochemistry of 36 normal human tissues and a broad range of tumor tissues to profile RAAG12 expression. Results.—More than 90% of colon, gastric, and pancreatic adenocarcinomas expressed RAAG12, and expression was uniform in most samples. Expression of RAAG12 at lower frequency and/or uniformity was observed in other cancers, including esophageal, ovarian, liver, breast, and prostate carcinomas and adenocarcinomas. Similar RAAG12 expression was observed between primary and metastatic colon adenocarcinomas. No staining was seen on cardiovascular, endocrine, neuromuscular, hematopoietic, or nervous system tissue from non–tumor-bearing individuals. RAAG12 was expressed on mucosal and glandular/ductal epithelium. The gastrointestinal tract mucosa and pancreatic/biliary ducts displayed the most uniform reactivity. RAAG12 exhibited differential subcellular localization in these normal, compared with tumor, tissues. Normal polarized epithelia primarily displayed apical membrane and cytoplasmic staining, whereas tumors exhibited whole membrane staining that increased with decreasing differentiation. Conclusions.—High expression of RAAG12 on tumors of gastrointestinal origin suggests these cancers are appropriate targets for RAV12 therapy. Differential subcellular location of RAAG12 on normal epithelia may limit accessibility of RAV12 to the subset of normal tissues that exhibit antigen expression.

scholarly journals Moral and Social Values Embodied in the Books Content of National Interactive Curriculum for Kindergartens in Jordan—An Analytical Study

2016 ◽  
Vol 6 (2) ◽  
pp. 216
Author(s):  
Reham M Al-Mohtadi ◽  
Eid H El-Subhieen ◽  
Ahmad A Al-khattab
Keyword(s):  
Analytical Study ◽  
Social Values ◽  
Personal Values ◽  
The Other ◽  
Analysis Tools ◽  
Other Hand ◽  
Interactive Curriculum

<p>The study aimed to discuss the moral and social values embodied in the books contents of national interactive curriculum for kindergartens in Jordan; through analyzing such books’ contents. For the purposes of this study, a list of proposed moral and social values was prepared, including twenty eight values. Word, phrase and image are considered herein as analysis tools. The Generated outputs referred to 360 values which are contained in the books of national interactive curriculum for kindergartens in Jordan. The National Social values field occupied the first rank of 256 repetitions, at 71.11%. On the other hand, family personal values field occupied the 2<sup>nd</sup>. rank; 104 repetitions at 28.89%. The outputs also displayed the availability of many suitable moral and social values stipulated for in the study tool. In turn, it was noticed the unavailability of many suitable moral and social values.</p>

TOXOHORMONE IN BACTERIA-FREE TUMORS

1966 ◽  
Vol 44 (8) ◽  
pp. 1069-1087 ◽  
Author(s):  
J. C. Nixon ◽  
B. Zinman
Keyword(s):  
Gel Filtration ◽  
Human Kidney ◽  
Metastatic Carcinoma ◽  
Exchange Chromatography ◽  
Human Spleen ◽  
Normal Tissues ◽  
Highly Active ◽  
Normal Human Kidney ◽  
Tumor Tissues ◽  
Normal Human

Toxohormone was extracted from bacteria-free human tumors and normal tissues, and assayed for activity by measuring the decrease in serum iron levels of rats 12 hours after injection of the extracts. In contrast with the findings of others, the results of the present study demonstrated that active toxohormone could be isolated from bacteria-free tumor tissues. Bacteria-free normal human kidney and spleen also yielded active toxohormone extracts, whereas extracts of normal human- and rat-skeletal muscle and rat liver had no activity.Four active toxohormone extracts were purified by ion-exchange chromatography followed by gel filtration. Human leukemic spleen, metastatic carcinoma of the cecum, and normal human spleen and kidney yielded several highly active purified fractions.

Concentrations of glucose, albumin, inorganic phosphate and sodium in the blood of beef calves at 2 ½ months of age and their relationships with subsequent weight gain

1980 ◽  
Vol 94 (1) ◽  
pp. 95-104 ◽  
Author(s):  
G. J. Rowlands
Keyword(s):  
Growth Rate ◽  
Blood Glucose ◽  
Growth Rates ◽  
Inorganic Phosphate ◽  
The Body ◽  
The Other ◽  
Growth Depression ◽  
Blood Samples ◽  
Beef Calves ◽  
Body Weights

SummaryBlood samples were taken at 9, 10 and 11 weeks of age from 230 male Hereford × Friesian calves, the progeny of 12 Hereford bulls. Concentrations of blood glucose, serum albumin, inorganic phosphate and sodium were measured and correlated with body weights and growth rates until slaughter at 19½ months of age.Correlations between growth rates and glucose concentrations (0·44) and between growth rates and albumin concentrations (0·38) were observed until 4 months of age. Similar correlations between body weights and blood glucose or albumin concentrations persisted until 6 months of age. By 10 months, however, the correlations had begun to decline, and by slaughter they had become insignificant.Correlations between growth rates and inorganic phosphate or sodium concentrations were smaller, and also decreased with age.The effect of hypoglycaemia on growth rate was compared with the effect of enzootic pneumonia. Although the 10% of calves with the lowest glucose concentrations were growing 24% more slowly than the other calves at the time of sampling, this growth depression was not related to pneumonia, and they subsequently made up for most of the early loss of growth.Three blood samples were also taken from 22 Hereford bulls. No significant correlations were observed between the blood composition of the bulls and the body weights or weight gains of their progeny.

scholarly journals Identification of key genes related to macrophage infiltration in gastric cancer based on WGCNA

2020 ◽  
Author(s):  
Haiyan Chen ◽  
Cangang Zhang ◽  
Shuai Cao ◽  
Meng Cao ◽  
Nana Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Immune Cell ◽  
Accurate Diagnosis ◽  
Marker Genes ◽  
Hub Genes ◽  
Normal Tissues ◽  
Tumor Tissues ◽  
New Strategy ◽  
Tumor Environment ◽  
Advanced Stages

Abstract Background: Gastric cancer (GC) is rampant around the world. Most of the GC cases are detected in advanced stages with poor prognosis. The identification of marker genes for early diagnosis is of great significance. Studying the tumor environment is helpful to acknowledge the process of tumorigenesis, development, and metastasis.Methods: In GEO, 22 kinds of immune cell infiltration were calculated by CIBERSORT. Macrophages were discovered remarkably infiltrated higher in GC compared with normal tissues. WGCNA was utilized to construct the network and then identify key modules and genes related to macrophages in TCGA.Results: Finally, 18 hub genes were verified. In the PPI bar chart, the top 3 genes were chosen as hub genes involved in most pathways. On the TIMER and THPA websites, it is verified that the expression levels of CYBB, CD86 and C3AR1 genes in tumor tissues were higher than those in normal tissues.Conclusion: These genes may work as biomarkers or targets for accurate diagnosis and treatment of GC in the future. Our findings may be a new strategy for the treatment of GC.

scholarly journals Interleukin 37 Expression was Negatively Associated with Pathological Grading in Human Gliomas

2021 ◽  
Author(s):  
Sheng Liu ◽  
Ying Ba ◽  
Chenglong Li ◽  
Mengyang Xing ◽  
Tao Zhang ◽  
...  
Keyword(s):  
Survival Time ◽  
Grade Level ◽  
Brain Injuries ◽  
Control Group ◽  
Normal Brain ◽  
Normal Tissues ◽  
Pathological Type ◽  
Tumor Tissues ◽  
Clinicopathologic Factors ◽  
Immunohistochemical Techniques

Abstract Background: Little is known about the roles of interleukin 37 (IL-37), a newly identified cytokine, in the pathogenesis of cancer. In this study, we aimed to determine the expression of IL-37 in gliomas with different pathological grades and evaluated its effects on survival. Methods: Ninety-five participants with different pathological grades of glioma were included in this study, which were classified into grade I-II (n=27), grade III (n=30), and grade IV (n=38). Ten normal brain tissues that were resected for intracranial decompression after traumatic brain injuries served as control group. The expression of IL-37 mRNA and protein in glioma tissues was determined using Real-time PCR and immunohistochemical techniques. The association between IL-37 expression and various clinicopathologic factors was evaluated. Results: IL-37 mRNA was expressed in normal tissues and tumor tissues, and the expression of IL-37 in tumor tissues were significantly higher than normal brain tissue (p<0.05). IL-37 expression showed decline with the increase of grade level. The expression of IL-37 was significantly lower in glioma tissues of a high malignancy compared with the glioma tissues of a low malignancy. Patients with low IL-37 expression showed a shorter survival time. Conclusions: Low IL-37 expression was negatively correlated with pathological grade, rather than pathological type. Low IL-37 was positively correlated with survival time. Thus, IL-37 maybe plays an inhibitory role in glioma progression.

scholarly journals Comparison of auxin activty in tumourous and normal callus cultures from sunflower and tobacco plants

2015 ◽  
Vol 48 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Z. Chirek
Keyword(s):  
Genetic Regulation ◽  
Callus Cultures ◽  
The Other ◽  
Hormone Metabolism ◽  
Tobacco Plants ◽  
Auxin Level ◽  
Avena Coleoptile ◽  
Auxin Activity ◽  
Normal Tissues ◽  
Different Types

In normal and tumourous calluses of sunflower and tobacco the level of extractable auxins was determined by <i>Avena coleoptile</i> straight growth test. Auxin activity was detected practically in two zones: I - at position with R<sub>f</sub> 0.2-0.4 and II - at position with R<sub>f</sub> 0.6-0.9. The tumour tissues of sunflower and tobacco plants, representing different types of neoplastic growth exhibit a 3 times higher auxin activity as compared with that of the corresponding normal tissues. Tobacco tissues, on the other hand, had a higher auxin level than the corresponding sunflower tissues and they exhibited different proportions in the activity of zones I and II, which points to a dominance of genetic regulation of hormone metabolism in these plants.

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