scholarly journals Formoterol decreases muscle wasting as well as inflammation in the rat model of rheumatoid arthritis

2016 ◽  
Vol 310 (11) ◽  
pp. E925-E937 ◽  
Author(s):  
Ana Belén Gómez-SanMiguel ◽  
Carolina Gomez-Moreira ◽  
María Paz Nieto-Bona ◽  
Carmen Fernández-Galaz ◽  
Maria Ángeles Villanúa ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Body Weight ◽  
Muscle Wasting ◽  
Body Weight Loss ◽  
Intradermal Injection ◽  
Cross Sectional ◽  
Akt Phosphorylation ◽  
Male Wistar Rats ◽  
And Control ◽  
Igfbp 3

Adjuvant-induced arthritis is an experimental model of rheumatoid arthritis that is associated with body weight loss and muscle wasting. β2-adrenergic receptor agonists are powerful anabolic agents that trigger skeletal muscle hypertrophy and have been proposed as a promising treatment for muscle wasting in human patients. The aim of this work was to determine whether formoterol, a selective β2-adrenoreceptor agonist, is able to ameliorate muscle wasting in arthritic rats. Arthritis was induced in male Wistar rats by intradermal injection of Freund's adjuvant. Control and arthritic rats were injected daily with 50 μg/kg sc formoterol or saline for 12 days. Body weight change, food intake, and arthritis index were analyzed. After euthanasia, in the gastrocnemius mRNA was analyzed by PCR, and proteins were analyzed by Western blotting. Arthritis decreased gastrocnemius weight, cross-sectional area, and myofiber size, whereas formoterol increased those variables in both arthritic and control rats. Formoterol decreased the external signs of arthritis as well as NF-κB(p65) activation, TNFα, and COX-2 levels in the gastrocnemius of arthritic and control rats. Those effects of formoterol were associated with a decreased expression of myostatin, atrogin-1, and MuRF1 and in LC3b lipidation. Arthritis increased the expression of MyoD, myogenin, IGF-I, and IGFBP-3 and -5 in the gastrocnemius. In control and in arthritic rats, treatment with formoterol increased Akt phosphorylation and myogenin levels, whereas it decreased IGFBP-3 expression in the gastrocnemius. These data suggest that formoterol has an anti-inflammatory effect and decreases muscle wasting in arthritic rats through increasing Akt activity and myogenin and decreasing myostatin, the p-NF-κB(p65)/TNF pathway, and IGFBP-3.

scholarly journals Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

2020 ◽  
Vol 20 (07) ◽  
pp. 16984-16996
Author(s):  
MMC Anyakudo ◽  
◽  
DO Adeniji ◽  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Morphological Features ◽  
High Protein ◽  
Control Group ◽  
Formulated Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats ◽  
And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

scholarly journals Dynamic Changes of Orexin A and Leptin in Obese Children during Body Weight Reduction

2007 ◽  
pp. 89-96
Author(s):  
J Bronský ◽  
J Nedvídková ◽  
H Zamrazilová ◽  
M Pechová ◽  
M Chada ◽  
...  
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Weight Reduction ◽  
Plasma Levels ◽  
Body Height ◽  
Body Weight Loss ◽  
Average Weight ◽  
Obese Children ◽  
Orexin A ◽  
Igfbp 3

In this study, we describe changes of plasma levels of the hypothalamic neuropeptide orexin A in obese children during the reduction of body weight and its relationship to other biochemical and anthropometrical parameters. We measured orexin A fasting plasma levels by the RIA method in 58 obese children--33 girls and 25 boys; mean age 13.1+/-0.38 years (range 7-18.5) before and after 5 weeks of weight-reduction therapy. Leptin, IGF-1, and IGFBP-3 levels were measured in all the subjects and were compared to orexin A levels and anthropometrical data. Average weight in subjects before weight-reduction was 74.2+/-2.79 kg and after weight-loss 67.4+/-2.60 kg (p<0.0001). Orexin A levels before the therapy were 33.3+/-1.97 pg/ml and after the therapy 51.7+/-3.07 pg/ml (p<0.0001). Levels of orexin A were not significantly different between girls and boys (p=0.7842). We found negative correlation between orexin A and age (r = -0.5395; p<0.0001), body height (r = -0.4751; p=0.0002), body weight (r = -0.4030; p=0.0017) and BMI (r = -0.2607; p=0.0481). No correlation was found between orexin A and IGF-1, IGFBP-3 or leptin. Orexin A plasma levels increased during body weight loss, whereas the reverse was true for leptin levels. These findings support the hypothesis that orexin A may be involved in regulation of nutritional status in children.

Effect of orexin-A (hypocretin-1) on hyperalgesic and cachectic manifestations of experimentally induced rheumatoid arthritis in rats

2014 ◽  
Vol 92 (10) ◽  
pp. 813-820 ◽  
Author(s):  
Adham R. Mohamed ◽  
Wessam F. El-Hadidy
Keyword(s):  
Rheumatoid Arthritis ◽  
Body Mass ◽  
Joint Destruction ◽  
Serum Levels ◽  
Intradermal Injection ◽  
Pain Sensation ◽  
Orexin A ◽  
Male Wistar Rats ◽  
Normal Controls ◽  
Inflammatory Effect

Orexin-A has been shown to modulate pain sensation and increase appetite. Rheumatoid arthritis (RA) is characterized by joint destruction, deformity, hyperalgesia, and weight reduction. Aim: Evaluate the possible effect of orexin-A on hyperalgesic and cachectic manifestations in an adjuvant-induced arthritis (AIA) rat model. Methods: Forty adult male Wistar rats were distributed among 4 groups; I, normal controls; II, rats with AIA induced by intradermal injection of Mycobacterium butyricum, but with no other treatment; III, AIA rats treated daily with an intravenous injection of orexin-A for 8 days; and IV, AIA rats treated orally with dexamethasone for 8 days. The parameters we assessed were pain-associated behavior, body mass, hind paw volume, serum levels of nerve growth factor (NGF) and neuropeptide Y (NPY). Results: Orexin-A caused a significant reduction in pain sensation and NGF levels, and increased body mass and the levels of NPY, whereas treatment with dexamethasone led to significant reductions in paw swelling and pain sensation. Conclusion: Orexin-A has hypoalgesic properties and increases body mass, whereas dexamethasone has a potent anti-inflammatory effect. Therefore, the combination of orexin-A and dexamethasone should have a greater effect with respect to attenuating the manifestations and complications associated with RA.

scholarly journals Body weight loss and associated factors among adults People living with HIV/AIDS on antiretroviral therapy in Shashemane referral Hospital, Oromia national regional state, Ethiopia: A facility based cross sectional Study

2020 ◽  
Author(s):  
Dedefo Bati Bedassa ◽  
Tsegaye Demissie Gamebo ◽  
Dereje Yohannes Teferi
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
Body Weight Loss ◽  
Cross Sectional Study ◽  
Referral Hospital ◽  
People Living With Hiv ◽  
Cross Sectional ◽  
Under Nutrition ◽  
Living With Hiv ◽  
Hiv Aids

Abstract Background: HIV/AIDS and under nutrition commonly manifested as body weight loss are both highly prevalent in many parts of the world. Their effects are interrelated and act in a vicious cycle. Both HIV and under nutrition can independently cause progressive damage to the immune system and increased susceptibility to infection. Ethiopia is one of the countries affected by both epidemics, despite, study done on acute under nutrition among HIV/AIDS adults in particular were found inadequate. Thus the main aim of this study is to assess the magnitude of acute under nutrition (weight loss within 3 months) and associated factors among people living with HIV/AIDS adults on ART in Shashemane referral Hospital. Methods: Facility based cross-sectional study conducted in shashemane referral Hospital Oromia region, Ethiopia from August 3 to September 4.2016. Systematic random sampling technique employed and 402 study participants were included. Data was collected by interview patients using pre-tested structured questionnaire, review of patients register, weight and height measurements were taken. The data were entered into Epi Info version 3.5.1 and analyzed using version 20 SPSS statistical package and percentages of body weight loss were calculated. Logistic regression analyses were carried out to identify factors associated with body weight loss of > 5% within three months. Strength of association determined using P< 0.05 and odd ratio (95% CI). Result: The magnitude body weight loss > 5% were 15.9% (95% CI; 12.4-19.7). Low CD4 level of less than 200mm3/ml (AOR=8.41,95% CI:3.46-20.44), inability to consume high protein diets like meat, egg & fish per week (AOR=2.97, 95% CI;1.39-6.35), low meal frequency of 2 or less per day (AOR=3.09,95% CI: 1.25-7.68) and low average income of 1000 birr per month (AOR=9.07,95% CI: 3.71-22.14) were significantly associated with Body weight loss of > 5% within 3 months with P<0.05. Conclusion: Body weight loss >5% within 3 months(acute under nutrition) is still a problem in people living with HIV/AIDS adults on ART, specifically among advanced immune compromised, low income, consumes less meal frequently per day and fewer or no consumption of animal product.

scholarly journals Androgenic Influences on Behavior, Body Weight, and Body Composition in a Model of Chronic Social Stress

Endocrinology ◽  
2007 ◽  
Vol 148 (12) ◽  
pp. 6145-6156 ◽  
Author(s):  
Mary M. N. Nguyen ◽  
Kellie L. K. Tamashiro ◽  
Susan J. Melhorn ◽  
Li Y. Ma ◽  
Stacy R. Gardner ◽  
...  
Keyword(s):  
Weight Loss ◽  
Body Composition ◽  
Body Weight ◽  
Adipose Tissue ◽  
Social Stress ◽  
Body Weight Loss ◽  
Oral Glucose ◽  
Lean Tissue ◽  
Chronic Social Stress ◽  
And Control

The visible burrow system (VBS) is a model used to study chronic social stress in colony-housed rats. A hierarchy develops among the males resulting in dominant (DOM) and subordinate (SUB) animals. Hierarchy-associated changes in body weight, body composition, behavior, and neuroendocrine measures have been observed. After 14 d of VBS housing, SUB animals have decreased body weight, elevated corticosterone, and decreased testosterone (T), compared with DOM animals and controls, placing SUB animals in an ideal endocrine state to regain lost body weight as adipose tissue. It is hypothesized that maintaining constant androgen concentrations in SUB males during stress will prevent body weight loss by maintaining more lean body mass. To test this, animals were gonadectomized and implanted with SILASTIC implants containing T, 5α-dihydrotestosterone (DHT), or cholesterol. Implants maintained constant physiological levels of T. Standard intact, T, and DHT implant colonies formed hierarchies, whereas cholesterol colonies did not. Androgen manipulations significantly altered offensive and defensive behaviors only on the first day of VBS housing. After VBS stress, intact, T, and DHT SUB animals weighed less and lost more adipose and lean tissue than DOM and control males, whereas DOM animals primarily lost adipose tissue. However, on recovery, DHT SUB animals maintained more lean tissue than intact SUB animals. Oral glucose tolerance tests revealed that glucose clears faster in stressed T-implanted males that have increased adipose tissue. Overall, these data suggest that constant androgen concentrations in SUB animals do not prevent weight loss and changes in body composition during stress but do so during recovery.

scholarly journals Nicotinamide attenuates streptozotocin-induced diabetes complications and increases survival rate in rats: role of autonomic nervous system

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Paula L. Cruz ◽  
Ivana C. Moraes-Silva ◽  
Amanda A. Ribeiro ◽  
Jacqueline F. Machi ◽  
Marcelo Dantas Tavares de Melo ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Survival Rate ◽  
Baroreflex Sensitivity ◽  
Diabetes Complications ◽  
Body Weight Loss ◽  
Diabetic Rats ◽  
Male Wistar Rats ◽  
Streptozotocin Induced Diabetes

Abstract Background To evaluate the effect of nicotinamide prior to streptozotocin-induced (STZ) diabetes in baroreflex sensitivity and cardiovascular autonomic modulation, and its association with hemodynamics and metabolic parameters. Methods Methods: Male Wistar rats were divided into control (Cont) and STZ-induced diabetes (Diab). Half of the rats from each group received a single dose of nicotinamide (100 mg/Kg) before STZ injection (Cont+NicA and Diab+NicA). All groups were followed-up for 5 weeks. Results Body weight loss of more than 40% was observed in Diab throughout the period (Diab: 271.00 ± 12.74 g; Diab+NicA: 344.62 ± 17.82). Increased glycemia was seen in Diab rats (541.28 ± 18.68 mg/dl) while Diab+NicA group had a slight decrease (440.87 ± 20.96 mg/dl). However, insulin resistance was observed only in Diab. In relation to Cont, heart rate, mean blood pressure and diastolic function were reduced when compared to Diab, together with parasympathetic modulation and baroreflex sensitivity. All of these parameters were improved in Diab+NicA when compared to Diab. Improved baroreflex sensitivity and parasympathetic modulation were correlated with glycemia, insulin resistance, and body weight mass. Additionally, Diab+NicA group increased survival rate. Conclusions Results suggest that the association of nicotinamide in STZ-induced diabetic rats prevents most of the expected derangements mainly by preserving parasympathetic and baroreflex parameters.

scholarly journals Intracerebroventricular injection of citrate inhibits hypothalamic AMPK and modulates feeding behavior and peripheral insulin signaling

2008 ◽  
Vol 198 (1) ◽  
pp. 157-168 ◽  
Author(s):  
Graziela R Stoppa ◽  
Maristela Cesquini ◽  
Erika A Roman ◽  
Patrícia O Prada ◽  
Adriana S Torsoni ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Body Weight ◽  
Adipose Tissue ◽  
Protein Kinase ◽  
Body Weight Loss ◽  
Control Group ◽  
Euglycemic Hyperinsulinemic Clamp ◽  
Akt Phosphorylation ◽  
Hypothalamic Cells ◽  
Feeding Control

We hypothesized that citrate might modulate the AMP-activated protein kinase/acetyl-CoA carboxylase (AMPK)/(ACC) pathway and participate in neuronal feeding control and glucose homeostasis. To address this issue, we injected citrate into the lateral ventricle of rats. Intracerebroventricular (ICV) injection of citrate diminished the phosphorylation of hypothalamic AMPK/ACC, increased the expression of anorexigenic neuropeptide (pro-opiomelanocortin and corticotropin-releasing hormone), elevated the level of malonyl-CoA in the hypothalamus, and reduced food intake. No change was observed in the concentration of blood insulin after the injection of citrate. With a euglycemic–hyperinsulinemic clamp, the glucose infusion rate was higher in the citrate group than in the control group (28.6±0.8 vs 19.3±0.2 mU/kg body weight/min respectively), and so was glucose uptake in skeletal muscle and the epididymal fat pad. Concordantly, insulin receptor (IR), IR substrate type 1 (IRS1), IRS2, and protein kinase B (AKT) phosphorylation in adipose tissue and skeletal muscle was improved by citrate ICV treatment. Moreover, the treatment with citrate for 7 days promoted body weight loss and decreased the adipose tissue. Our results suggest that citrate and glucose may serve as signals of energy and nutrient availability to hypothalamic cells.

scholarly journals Obesity and Prevalence of Latent Tuberculosis: A Population-Based Survey

2021 ◽  
Vol 14 ◽  
pp. 117863372199460
Author(s):  
Alaa Badawi ◽  
Christina J Liu
Keyword(s):  
Body Weight ◽  
Risk Factor ◽  
Adult Population ◽  
Latent Tuberculosis ◽  
Population Based ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Logistic Regression Models ◽  
Cardiometabolic Risk Markers ◽  
And Control

Objective: Diminution in body weight is a known risk factor that increases the burden of active tuberculosis (TB). However, conflicting evidence exists for the effect of body weight on the risk of latent tuberculosis infection (LTBI). The objective of the present study is to examine the prevalence of LTBI at different body weights, evaluate the extent of association between body mass index (BMI) and LTBI and identify factors mediating this relationship in an adult population. Methods: We conducted a cross-sectional study to estimate the relationship between BMI and LTBI in participants from the US-National Health and Nutrition Examination Survey (NHANES; 2012, n = 5156; 514 with LTBI and 4642 controls). Results: The association between BMI and levels of cardiometabolic risk markers in both LTBI and control groups had a similar profile. When adjusted for age and sex, BMI was significantly inversely correlated with the prevalence of LTBI ( r = −0.147, P < .001). Effect of BMI on the risk of LTBI was evaluated using multivariate logistic regression models adjusted for age, sex, diabetes, and level of education. In this model, increasing BMI was significantly associated with lower risk of LTBI (OR = 0.85; 95%CI: 0.77-0.96, P < .01). Conclusion: This study further establishes an inverse relationship between BMI and prevalence of LTBI. Decreased BMI can be considered as a risk factor in LTBI, the reservoir for active TB cases.

scholarly journals Association between previous rheumatoid arthritis and COVID-19 and its severity: a nationwide cohort study in South Korea

BMJ Open ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. e054753
Author(s):  
Younghee Jung ◽  
Mihye Kwon ◽  
Hyo Geun Choi
Keyword(s):  
Rheumatoid Arthritis ◽  
Primary Outcome ◽  
Cross Sectional Study ◽  
Secondary Outcome ◽  
Diagnostic Code ◽  
Cross Sectional ◽  
International Classification Of Disease ◽  
Laboratory Confirmation ◽  
History Of ◽  
And Control

ObjectivesThe prevalence of rheumatoid arthritis (RA) among patients with COVID-19 and the association between RA and the outcome of COVID-19 remain unclear. We aimed to compare the prevalence of RA between participants with and without COVID-19; we then analysed the association between the presence of RA and the severity of COVID-19.DesignA cross-sectional study.SettingData from a nationwide COVID-19 cohort database by the Korea National Health Insurance Corporation were used.Participants and interventionsA total of 8070 patients with COVID-19 (1 January 2020 through 4 June 2020) were matched with 32 280 control participants with regard to age, sex and income. Patients with COVID-19 were confirmed by SARS-CoV-2 PCR and controls were collected from the database. RA was confirmed using the diagnostic code (International Classification of Disease, Tenth Revision) and medication claim codes. Conditional/unconditional logistic regression was applied to analyse the association between RA and COVID-19.Primary outcome and secondary outcomeLaboratory confirmation of SARS-CoV-2 infection was defined as the primary outcome. The secondary outcome was severe COVID-19 defined as a history of intensive care unit admission, invasive ventilation or death.ResultsThe prevalence of RA in the COVID-19 (0.4%, 35/8070) and control (0.4%, 121/32,280) groups did not differ (p=0.446). After adjusting for underlying diseases, no association between RA and COVID-19 was observed (adjusted OR=1.14, 95% CI: 0.78 to 1.67) and COVID-19 severity was not associated with RA (adjusted OR=0.62, 95% CI: 0.14 to 7.29). The overall mortality rate was 2.9% (237/8070) and RA was not significantly associated with mortality (adjusted OR=1.64, 95% CI: 0.33 to 8.15).ConclusionWe did not find an association between the presence of RA and COVID-19. In addition, RA was not associated with the severity of COVID-19.

scholarly journals Participation in physical activity decreased more in people with rheumatoid arthritis than the general population during the COVID-19 lockdown: a cross-sectional study

2021 ◽  
Author(s):  
Christopher Balchin ◽  
Ai Lyn Tan ◽  
Oliver J. Wilson ◽  
Jim McKenna ◽  
Antonios Stavropoulos-Kalinoglou
Keyword(s):  
Rheumatoid Arthritis ◽  
Physical Activity ◽  
Body Weight ◽  
Online Survey ◽  
Cross Sectional Study ◽  
Mental Wellbeing ◽  
Cross Sectional ◽  
The Impact ◽  
Exercise Equipment

AbstractThe COVID-19 pandemic and social distancing restrictions have significantly reduced population-wide physical activity (PA) levels. However, the impact of the pandemic and relevant restrictions on PA participation, and any potential barriers to it, in people with rheumatoid arthritis (RA) are not clear. Furthermore, we are unsure if any such PA changes have affected their body weight, mental wellbeing, and/or quality of life (QoL). Thus, the aim of this study was to examine the impact of the lockdown on PA participation in people with RA, versus people without RA. Participants (n = 128; RA = 27, non-RA = 101) completed a self-administered online survey, which included questions on PA, body weight, mental wellbeing and QoL. PA participation during lockdown was significantly lower among RA versus non-RA participants (p < 0.001). Additionally, a similar profile of results was found where more RA participants vs non-RA participants reported reduced habitual PA (59% vs 33%) and increased body weight (59% vs 35%). Mental wellbeing scores were similarly low in both groups during lockdown (RA: 20.8 ± 4.2; non-RA: 22.2 ± 3.4, p = 0.080). Matched group comparisons identified similar trends to full sample analyses. In the first months of the lockdown, more people with RA reported decreased PA participation and increased body weight than their non-RA counterparts. Access to exercise equipment and facilities appears to be the main cause for these results. Looking beyond COVID-19, specific PA promotion for people with RA will be required to prevent a pandemic of inactivity.

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