scholarly journals Applications of Infrared and Raman Microspectroscopy of Cells and Tissue in Medical Diagnostics: Present Status and Future Promises

2012 ◽  
Vol 27 ◽  
pp. 463-496 ◽  
Author(s):  
Max Diem ◽  
Miloš Miljković ◽  
Benjamin Bird ◽  
Tatyana Chernenko ◽  
Jen Schubert ◽  
...  
Keyword(s):  
Data Analysis ◽  
Vibrational Spectra ◽  
Medical Diagnostics ◽  
Cellular Biology ◽  
Steady Increase ◽  
Research Groups ◽  
The Past ◽  
New Research ◽  
Raman And Ir Spectroscopy

This paper summarizes the progress achieved over the past fifteen years in applying vibrational (Raman and IR) spectroscopy to problems of medical diagnostics and cellular biology. During this time, a number of research groups have verified the enormous information content of vibrational spectra; in fact, genomic, proteomic, and metabolomic information can be deduced by decoding the observed vibrational spectra. This decoding process is aided enormously by the availability of high-power computer workstations and advanced algorithms for data analysis. Furthermore, commercial instrumentation for the fast collection of both Raman and infrared microspectral data has rendered practical the collection of images based solely on spectral data. The progress in the field has been manifested by a steady increase in the number and quality of publications submitted by established and new research groups in vibrational biological and biomedical arenas.

scholarly journals Seismic Data Denoising Based on Sparse and Low-Rank Regularization

Energies ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 372 ◽  
Author(s):  
Shu Li ◽  
Xi Yang ◽  
Haonan Liu ◽  
Yuwei Cai ◽  
Zhenming Peng
Keyword(s):  
Data Analysis ◽  
Seismic Data ◽  
Spatial Information ◽  
Local Area ◽  
Low Rank ◽  
Self Similarity ◽  
Denoising Method ◽  
The Past ◽  
And Inversion

Seismic denoising is a core task of seismic data processing. The quality of a denoising result directly affects data analysis, inversion, imaging and other applications. For the past ten years, there have mainly been two classes of methods for seismic denoising. One is based on the sparsity of seismic data. This kind of method can make use of the sparsity of seismic data in local area. The other is based on nonlocal self-similarity, and it can utilize the spatial information of seismic data. Sparsity and nonlocal self-similarity are important prior information. However, there is no seismic denoising method using both of them. To jointly use the sparsity and nonlocal self-similarity of seismic data, we propose a seismic denoising method using sparsity and low-rank regularization (called SD-SpaLR). Experimental results showed that the SD-SpaLR method has better performance than the conventional wavelet denoising and total variation denoising. This is because both the sparsity and the nonlocal self-similarity of seismic data are utilized in seismic denoising. This study is of significance for designing new seismic data analysis, processing and inversion methods.

Methods for detecting autophagy and determining autophagy-induced cell deathThis review is one of a selection of papers published in a Special Issue on Oxidative Stress in Health and Disease.

2010 ◽  
Vol 88 (3) ◽  
pp. 285-295 ◽  
Author(s):  
Yongqiang Chen ◽  
Meghan B. Azad ◽  
Spencer B. Gibson
Keyword(s):  
Cell Death ◽  
Degradation Process ◽  
Research Groups ◽  
Special Issue ◽  
Lysosomal Degradation ◽  
The Past ◽  
Promote Cell Survival ◽  
Health And Disease ◽  
New Research ◽  
Selection Of

Autophagy is an intracellular lysosomal degradation process, which in the case of macroautophagy, is characterized by the formation of double-membraned autophagosomes. Enhanced under stress conditions, autophagy can function to promote cell survival or cell death depending on the type of cellular stress. Interest in autophagy has increased substantially in the past several years as new research implicates this “self-eating” pathway in cell growth, development, and many human diseases. Various methods have been developed for detecting autophagy; however, the implementation of these methods and the interpretation of the results often vary between studies, and a more standardized approach is required. In this review, we summarize the current methods available for detecting autophagy and for determining its contribution to cell death. Furthermore, we discuss the critical points for the successful application of these methods based on experiences from our laboratory and from other research groups.

Structure and relationships of basal relictual angiosperms

2004 ◽  
Vol 17 (4) ◽  
pp. 343 ◽  
Author(s):  
Peter K. Endress
Keyword(s):  
Research Methods ◽  
Past Century ◽  
Research Groups ◽  
Basal Angiosperms ◽  
The Past ◽  
New Research ◽  
New Framework

A review of the present perspective of basal angiosperms and the steps that led to this perspective is provided. Major steps of progress over the past century can be seen with the discovery of new extant plants (Degeneria), new fossils and the advent of new research methods, especially molecular cladistic studies, which catalysed new waves of research. A spectacular result in 1999 was the identification of the well-supported basalmost clades of the angiosperms, forming the ANITA grade, with Amborella, Nymphaeales and Austrobaileyales, by several research groups. This provided a new framework in which basal angiosperms can be discussed in evolutionary terms. In this review basalmost angiosperms (ANITA grade and Chloranthaceae) and other basal angiosperms (eumagnoliids and Ceratophyllaceae) are addressed, with a focus on the different major clades and on general traits and evolutionary tendencies. A short outlook for future avenues is also provided.

The Past and Future of the Scientific Study of International Processes

2018 ◽  
Author(s):  
Zeev Maoz
Keyword(s):  
Scientific Discipline ◽  
Scientific Study ◽  
Data Availability ◽  
Policy Implications ◽  
Policy Makers ◽  
The Past ◽  
Substantial Progress ◽  
New Research ◽  
Research Frontiers

The scientific study of international processes (SSIP) has made substantial progress over the past twenty years, establishing itself as the mainstream research community in the field of international relations (IR) and attracting more and more attention from other disciplines. This was due to the convergence of several revolutions that have taken place in the field, including the data revolution, the formal modeling revolution, the methods revolution, the substantive revolution, and the epistemological revolution. In addition to the dramatic increase in the number of the community of scholars who use scientific logic, systematic methods, and empirical data to study IR, there was a significant improvement in the quality of research. This research has yielded important contributions to our understanding of international processes. Some of these contributions went far beyond the field; they have attracted the attention of policy makers as well as quite a few scholars from other disciplines. Some of the key findings that emerged from this research have become—correctly or incorrectly—a key component of the discourse of political leaders. Growing data availability, increased methodological sophistication, and greater scientific discipline within the profession have converged to open new research frontiers, but important challenges remain, such as the disconnect between theory and empirical tests that exists in many cases, and the almost exclusive reliance on the dyadic level of analysis. It is important to make our understanding of international processes translated into broader policy implications.

Journal Publication Performance of Economic History Programs: 1960–1969 and 1970–1974

1975 ◽  
Vol 35 (3) ◽  
pp. 635-641 ◽  
Author(s):  
Albert W. Niemi
Keyword(s):  
Economic History ◽  
Graduate Programs ◽  
Academic Institutions ◽  
Journal Publication ◽  
The Past ◽  
Journal Publications ◽  
Research Findings ◽  
New Research

This paper reviews the output of journal publications by the twenty five leading economic history programs during the past fifteen years. At present, there are no formal ratings of the quality of economic history programs at the various academic institutions, but journal publications can serve as a partial indicator of quality. The Roose-Andersen ratings graduate programs provide separate rankings of economics and history programs, but this information may not be appropriate in evaluating economic history programs, and the ratings have not been updated since 1969. Journal publications are certainly not the only measure of quality academic institutions, but journals are regarded as the standard outlet for disseminating new research findings and expanding the frontiers of knowledge. Those institutions that have concentrated their publication efforts on books, textbooks, monographs, working papers, and other nonjournal forms of publication will of course be underrepresented by the procedures followed in this paper.

scholarly journals The danger of epigenetics misconceptions (epigenetics and stuff…)

2015 ◽  
Vol 93 (6) ◽  
pp. 626-629 ◽  
Author(s):  
Philippe T. Georgel
Keyword(s):  
Gene Expression ◽  
Structure And Function ◽  
Epigenetic Mechanisms ◽  
Research Groups ◽  
Post Translational Modifications ◽  
The Past ◽  
Associated Proteins ◽  
New Research ◽  
The Right ◽  
And Function

Within the past two decades, the fields of chromatin structure and function and transcription regulation research started to fuse and overlap, as evidence mounted to support a very strong regulatory role in gene expression that was associated with histone post-translational modifications, DNA methylation, as well as various chromatin-associated proteins (the pillars of the “Epigenetics” building). The fusion and convergence of these complementary fields is now often simply referred to as “Epigenetics”. During these same 20 years, numerous new research groups have started to recognize the importance of chromatin composition, conformation, and its plasticity. However, as the field started to grow exponentially, its growth came with the spreading of several important misconceptions, which have unfortunately led to improper or hasty conclusions. The goal of this short “opinion” piece is to attempt to minimize future misinterpretations of experimental results and ensure that the right sets of experiment are used to reach the proper conclusion, at least as far as epigenetic mechanisms are concerned.

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

Motilitas dan Vabilitas Spermatozoa Ayam Kampung pada Suhu 5°C Menggunakan Pengencer dan Lama Simpan yang Berbeda

2018 ◽  
Vol 5 (2) ◽  
pp. 102
Author(s):  
Enike Dwi Kusumawati ◽  
Selvinus Lawu Woli ◽  
Aju Tjatur Nugroho Krisnaningsih ◽  
Waluyo Edi Susanto ◽  
Syam Rahadi
Keyword(s):  
Data Analysis ◽  
Storage Temperature ◽  
Coconut Water ◽  
Laboratory Research ◽  
Ringer Lactate ◽  
Randomized Design ◽  
Native Chicken ◽  
Lactate Solution ◽  
Better Than

ABSTRAKPenelitian ini dilakukan untuk mengetahui motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5oC menggunakan pengencer dan lama simpan yang berbeda. Metode yang digunakan dalam penelitian ini adalah penelitian laboratorium menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan pengencer ringer lactat solution, air kelapa dan tanpa pengencer serta lama simpan 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, masing-masing diulang 10 kali. Variabel yang diamati yaitu motilitas dan viabilitas spermatozoa. Analisa data yang digunakan adalah analisis varian. Hasil analisis data menunjukkan bahwa motilitas dan viabilitas spermatozoa menggunakan pengencer ringer lactat solution lebih tinggi (P<0,01) serta dapat bertahan sampai lama simpan 24 jam dibandingkan air kelapa dan tanpa pengencer. Adapun nilai motilitas ringer lactat solution, air kelapa dan tanpa pengencer pada lama simpan 24 jam masing-masing sebesar 43,5±17,17%; 8±4,83%; 6,5±2,4%, sedangkan nilai viabilitasnya sebesar 83,2±7,25%; 64,6±3,20%; dan 63,1±2,33%. Kesimpulan dari hasil penelitian ini adalah ringer lactat solution lebih baik dibandingkan air kelapa dan tanpa pengencer dalam mempertahankan kualitas semen ayam kampung pada suhu simpan 5oC sampai lama simpan 24 jam.Kata Kunci : air kelapa, ayam kampung, motilitas, spermatozoa, viabilitas  ABSTRACTThis study was conducted to determine the motility and viability of spermatozoa of Native chickens at 5oC using different diluents and time storage. The method used in this study was laboratory research using Factorial Completely Randomized Design with ringer lactate solution, coconut water and without diluent at 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30 hours of time storage each repeated 10 times. The variables observed were motility and viability of sperm. Data analysis used is variance analysis. The results of data analysis showed that the motility and viability of spermatozoa using ringer lactate solution diluents was higher (P <0.05) than coconut water and without diluents. The motility values of ringer lactat solution, coconut water and without diluents were 43,5±17,17%; 8±4,83%; 6,5±2,4% respectively, while the viability values were 83,2±7,25%; 64,6±3,20% and 63,1±2,33%. The conclusion of this study is that ringer lactat solution is better than coconut water an without diluents in maintaining the quality of Native chicken semen at a storage temperature of 5oC until 24 hours.Keywords: coconut water, motility, native chicken, sperm, viability

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

Exercise Capacity in Chronic Heart Failure

2012 ◽  
Vol 9 (1) ◽  
pp. 57-60 ◽  
Author(s):  
Gina G Mentzer ◽  
Alex J Auseon
Keyword(s):  
Quality Of Life ◽  
Heart Failure ◽  
Exercise Training ◽  
Exercise Capacity ◽  
Positive Impact ◽  
Pharmacologic Therapy ◽  
The Past ◽  
Diagnosis And Prognosis ◽  
Future Therapy

Heart failure (HF) affects more than 5 million people and has an increasing incidence and cost burden. Patients note symptoms of dyspnea and fatigue that result in a decreased quality of life, which has not drastically improved over the past decades despite advances in therapies. The assessment of exercise capacity can provide information regarding patient diagnosis and prognosis, while doubling as a potential future therapy. clinically, there is acceptance that exercise is safe in hf and can have a positive impact on morbidity and quality of life, although evidence for improvement in mortality is still lacking. specific prescriptions for exercise training have not been developed because many variables and confounding factors have prevented research trials from demonstrating an ideal regimen. Physicians are becoming more aware of the indices and goals for hf patients in exercise testing and therapy to provide comprehensive cardiac care. it is further postulated that a combination of exercise training and pharmacologic therapy may eventually provide the most benefits to those suffering from hf.

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