scholarly journals Hepatoprotective Effects ofSilybum marianum(Silymarin) andGlycyrrhiza glabra(Glycyrrhizin) in Combination: A Possible Synergy

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mahmood Rasool ◽  
Javed Iqbal ◽  
Arif Malik ◽  
Hafiza Sobia Ramzan ◽  
Muhammad Saeed Qureshi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Injury ◽  
Liquid Paraffin ◽  
Thiobarbituric Acid ◽  
Positive Control ◽  
Albino Rats ◽  
Dosage Regimens ◽  
Group A ◽  
Hepatoprotective Effects ◽  
Different Doses

Oxidative stress, lipid peroxidation, and transaminase reactions are some of the mechanisms that can lead to liver dysfunction. A time-dependent study was designed to evaluate the ability of silymarin (SLN) and glycyrrhizin (GLN) in different dosage regimens to lessen oxidative stress in the rats with hepatic injury caused by the hepatotoxin carbon tetrachloride. Wistar male albino rats (n= 60) were randomly assigned to six groups. Group A served as a positive control while groups B, C, D, E, and F received a dose of CCl4(50% solution of CCl4in liquid paraffin, 2 mL/kg, intraperitoneally) twice a week to induce hepatic injury. Additionally, the animals received SLN and GLN in different doses for a period of six weeks. CCl4was found to induce hepatic injury by significantly increasing serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and thiobarbituric acid reactive substances while decreasing total protein and the activities of reduced glutathione, superoxide dismutase, and catalase. Treatment with various doses of SLN and GLN significantly reduced ALT, AST, ALP, and TBARS levels and increased GSH, SOD, and CAT levels. Our findings indicated that SLN and GLN have hepatoprotective effects against oxidative stress of the liver.

scholarly journals Studies on Ameliorative Effects of Polyphenolic Extract from Paullinia pinnata L. (Sapindaceae) on Carbon Tetrachloride - Induced Hepatotoxicity and Oxidative Stress: an in vivo Assessment

2018 ◽  
Vol 10 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Mikhail O. NAFIU ◽  
Sefiu S. OLANIYI ◽  
Musa O. SALAWU ◽  
Abdulwakeel Ayokun-nun AJAO ◽  
Musbau A. AKANJI
Keyword(s):  
Oxidative Stress ◽  
Liver Function ◽  
Normal Saline ◽  
Defense Mechanisms ◽  
Liquid Paraffin ◽  
Albino Rats ◽  
Once Daily ◽  
Polyphenolic Extract ◽  
Group A

The current study investigates the effects of polyphenolic extract from the leaf of Paullinia pinnata against CCl4 – induced oxidative stress and liver damage in female albino rats. Thirty albino rats were randomly distributed into six groups (A-F). Rats in group A were given 1 ml normal saline orally to serve as control. The rats in groups B, C, D, E, and F were respectively induced intraperitoneally with single administration of 1 ml/kg body weight (b. wt) CCl4 dissolved in liquid paraffin (1:1). Thirty minutes after induction, the rats in the respective groups were orally treated with normal saline, 50 mg/kg b. wt. Silymarin, 50, 100 and 200 mg/kg b. wt. polyphenolic extract from P. pinnata respectively, once daily for 7 days.  Levels of liver function indices and the activities of antioxidant enzymes were determined. Administration of polyphenolic extract from P. pinnata significantly (p < 0.05) ameliorated CCl4- induced hepatotoxicity with respect to liver function indices, antioxidant and lipid peroxidation parameters. The biochemical changes observed were also consistent with histopathological observations on the rat liver, as architectural degeneration and severe cellular necrosis were restored after the administration of polyphenolic extract from P. pinnata in the treated groups. The study suggests that polyphenolic extract from P. pinnata is a potential hepatoprotective agent against CCl4-mediated hepatic injury through fortification of antioxidant defense mechanisms.

scholarly journals Appraisal of the Antioxidative Potential of Aloe Barbadensis M. on Alcohol-Induced Oxidative Stress

2019 ◽  
Vol 63 (3) ◽  
pp. 34-46 ◽  
Author(s):  
D. I. Akinloye ◽  
R. N. Ugbaja ◽  
O. A. Dosumu
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Distilled Water ◽  
Oxidative Potential ◽  
Aloe Barbadensis ◽  
Group A ◽  
Oxidative Effects

Abstract This investigation estimated the anti-oxidative potential of Aloe barbadensis gel extracts in rats against alcohol-induced oxidative stress. Thirty male albino rats (5 each per group) were included in the experiments. Group A (positive control) and B (negative control) were administered 4 mg.kg–1 body weight distilled water and 50 % alcohol respectively for 21 days. Groups C and D were administered 50 % alcohol for the first 14 days followed by co-administration of 125 mg and 250 mg.kg−1 body weight extract with alcohol respectively for the last 7 days. Groups E and F were administered distilled water for the first 14 days followed by co-administration of 125 and 250 mg.kg−1 body weight Aloe barbadensis gel extracts with distilled water respectively for the last 7 days. The administration of alcohol resulted in a significant (P < 0.05) decrease in the specific activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH) levels, while cholesterol (CHO), triglycerides (TAG), nitric oxide (NO) and malondialdehyde (MDA) concentrations were significantly increased when compared to the controls. Co-mobilization with Aloe barbadensis gel extracts for 7 days significantly reversed the deleterious effects of alcohol in the treated groups when compared to the alcohol group. This study indicated that Aloe barbadensis probably possesses anti-oxidative effects against alcohol–induced oxidative stress in rats.

scholarly journals Ameliorative Effect of the Methanol Extract of Napoleonae imperialis Leaves against Methotrexate-induced Renal Damage in Albino Rats

2020 ◽  
pp. 10-20
Author(s):  
O. J. Mba ◽  
G. S. Aloh ◽  
E. N. Uhuo
Keyword(s):  
Normal Control ◽  
Renal Damage ◽  
Biochemical Analysis ◽  
Methanol Extract ◽  
Positive Control ◽  
Control Group ◽  
Albino Rats ◽  
Ameliorative Effect ◽  
Group A ◽  
Group B

Aim: This study was aimed to evaluate the effect of methanol extract of Napoleonae imperialis leaves against methotrexate renal damage in albino rats. Methodology: Thirty (30) male albino rats of mean weight 130 g were used for this study. The animals for the study were grouped into five (5) of six (6) rats each. Group A (normal control) received feed and water only and Group B (positive control) was induced with methotrexate without treatment. Test groups (C and D) were orally given 250 mg and 500 mg/kg b.wt of leaves extract, and group E was orally given the extract only (500 mg/kg b.wt) respectively for 14 days. All the rats used in this study were initially subjected to renal damage using 0.5 ml/kg of methotrexate except the normal control group. The rats were sacrificed after 14 days and the blood samples were collected for biochemical analysis, Results: From the result obtained, there was a significant (p< 0.05) decrease in the groups that received 250 mg/kg and 500 mg/kg b.wt of the plant extract in (Urea, Creatinine and Na+), and a significant increase (p< 0.05) in K+ and Cl-. Also there was a significant (p< 0.05) decrease in (Urea, Creatinine, and Na+) in comparison with the control groups and the group that received the extract only (500 mg/kg b.wt), and a significant increase (p< 0.05) in (K+ and Na+). Conclusion: The study indicates that the methanol extract of Napoleonae imperialis leaves may have exerted renal functioning effects in albino rats, and may also be used pharmacologically in the management of organ toxicity.

scholarly journals Hepatoprotective effects of Tagetes lucida root extract in carbon tetrachloride-induced hepatotoxicity in Wistar albino rats through amelioration of oxidative stress

2021 ◽  
Vol 59 (1) ◽  
pp. 986-997
Author(s):  
Samah Ali El-Newary ◽  
Rasha Fouad Ismail ◽  
Nermeen Mohammed Shaffie ◽  
Saber Fayez Hendawy ◽  
Elsayed Omer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Albino Rats ◽  
Root Extract ◽  
Hepatoprotective Effects ◽  
Wistar Albino Rats

scholarly journals Assessment of the Antioxidant Potential of Hypoestes rosea Leaf in Lead-acetate-induced Albino Rats

2020 ◽  
pp. 45-55
Author(s):  
F. K. Uwikor ◽  
E. O. Nwachuku ◽  
F. Igwe ◽  
E. S. Bartimaeus
Keyword(s):  
Oxidative Stress ◽  
Lead Acetate ◽  
Positive Control ◽  
Post Treatment ◽  
Albino Rats ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Pre Treatment ◽  
The Impact ◽  
Dose Dependent

Hypoestes rosea has been used as a traditional medicine in the Niger delta for dysfunction of the endocrine system. However, there has been no known study on the effects of hypoestes rosea on oxidative stress. In this study we evaluated the effect of aqueous extract of Hypoestes rosea (AEHR) leaf on oxidative stress markers of lead acetate induced male and female albino rats at acute and sub-chronic stages in pre-treatment and post-treatment phases. Animals were divided into 17 groups of five each for both sexes in the treatment groups, while the positive control group had 10 animals in each sex. 8 groups were for the acute phase of the study for 21 days in each sex, while 8 were for 35 days for the sub chronic stage of the study. Negative Control (NC) group received rat feed only, Experimental (EC) group received 100 mg/kg bwt/day for 21 days at acute and 35 days for sub chronic.  Positive Control (PC) group received 60mg/kg b.wt per day of lead acetate for 35 days. The other 3 groups received 100 mgkg, 200 mg/kg and 300 mg/kg b. wt respectively for 14 and 28 days either as pre treatment or post treatment, for both sexes of the albino rats. Samples were taken at the end of the study period through the jugular vein under chloroform anaesthesia. Results showed lead acetate induced oxidative stress in the rats, evidenced by the significantly decreased (p < 0.05) Superoxide Dismutase (SOD) and Total Antioxidant Capacity (TAC) between the NC and PC groups. The plant in a dose dependent pattern was able to significantly (p < 0.05), reverse the effect of lead acetate in the Post and pre treatment phases. Our study also shows that dose dependent AEHR extract significantly reduced the impact of lead in oxidative stress markers. In conclusion, consumption of AEHR by albino rats could help protect against lead acetate induced oxidative stress.

scholarly journals Choline and Cystine Deficient Diets in Animal Models with Hepatocellular Injury: Evaluation of Oxidative Stress and Expression of RAGE, TNF-α, and IL-1β

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Juliana Célia F. Santos ◽  
Orlando R. P. de Araújo ◽  
Iara B. Valentim ◽  
Kívia Queiroz de Andrade ◽  
Fabiana Andréa Moura ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Animal Models ◽  
Hepatic Steatosis ◽  
Hepatic Injury ◽  
Young Male ◽  
Thiobarbituric Acid ◽  
Hepatocellular Injury ◽  
Oxidative Stress Biomarkers ◽  
Tnf Α ◽  
Male Wistar Rats

This study aims to evaluate the effects of diets deficient in choline and/or cystine on hepatocellular injury in animal models (young male Wistar rats, aged 21 days), by monitoring some of the oxidative stress biomarkers and the expression of RAGE, TNF-α, and IL-1β. The animals were divided into 6 groups (n=10) and submitted to different diets over 30 days: AIN-93 diet (standard, St), AIN-93 choline deficient (CD) diet and AIN-93 choline and cystine deficient (CCD) diet, in the pellet (pl) and powder (pw) diet forms. Independently of the diet form, AIN-93 diet already led to hepatic steatosis and CD/CCD diets provoked hepatic damage. The increase of lipid peroxidation, represented by the evaluation of thiobarbituric acid reactive species, associated with the decrease of levels of antioxidant enzymes, were the parameters with higher significance toward redox profile in this model of hepatic injury. Regarding inflammation, in relation to TNF-α, higher levels were evidenced inCD(pl), while, for IL-1β, no significant alteration was detected. RAGE expression was practically the same in all groups, with exception ofCCD(pw)versusCCD(pl). These results together confirm that AIN-93 causes hepatic steatosis and choline and/or cysteine deficiencies produce important hepatic injury associated with oxidative stress and inflammatory profiles.

Oxidative stress and changes in adenosine deaminase activity of cattle experimentally infected by Fasciola hepatica

Parasitology ◽  
2017 ◽  
Vol 144 (4) ◽  
pp. 520-526 ◽  
Author(s):  
ALEKSANDRO S. DA SILVA ◽  
MATHEUS D. BALDISSERA ◽  
NATHIELI B. BOTTARI ◽  
MATHEUS E. GABRIEL ◽  
LEANDRO A. RHODEN ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adenosine Deaminase ◽  
Liver Tissue ◽  
Fasciola Hepatica ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Adenosine Deaminase Activity ◽  
Group A ◽  
Group B

SUMMARYThe aim of this study was to evaluate the oxidative stress in serum and liver and adenosine deaminase (ADA) activity of cattle experimentally infected by Fasciola hepatica. The group A consisted of five healthy animals (uninfected), and the group B was composed of five animals orally infected with 200 metacercariae of F. hepatica. On days 20, 40, 60 and 80 post-infection (PI) serum was collected to measure oxidative stress variables. On day 100 PI, animals were humanely euthanized and liver samples were collected. Infected animals showed lower (P < 0·05) seric ADA activities on days 40 and 60 PI but higher (P < 0·05) in the liver tissue compared with uninfected animals. Seric and hepatic reactive oxygen species (ROS) were higher (P < 0·05) in infected compared with uninfected animals. Hepatic thiobarbituric acid reactive substances were higher (P < 0·05) in infected animals. Catalase and glutathione S-transferase activities were lower in liver tissue of infected animals, while glutathione peroxidase was higher compared with uninfected (P < 0·05). In summary, we conclude that oxidative stress occurs in cattle experimentally infected by F. hepatica, mainly due to excessive ROS production in the course of fasciolosis, contributing to hepatic damage, and that increased in hepatic ADA activity may contribute to the inflammatory process.

Carbon Tetrachloride-Induced Hepatotoxicity: Protective Effect of 'Rocket' Eruca sativa L. in Rats

2010 ◽  
Vol 38 (01) ◽  
pp. 75-88 ◽  
Author(s):  
Saleh Alqasoumi
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Liver Tissue ◽  
Hepatic Injury ◽  
Liver Toxicity ◽  
Elevated Serum ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Eruca Sativa ◽  
Protein Sulfhydryl

The hepatoprotective and antioxidant effect of an ethanolic extract of 'Rocket' Eruca sativa L. (EER), on liver injury induced by carbon tetrachloride ( CCl4 ) was investigated. Wistar albino rats were administered 250 and 500 mg/kg body weight extract orally for 10 consecutive days. Marker enzymes GOT, GPT, ALP, GGT and bilirubin were estimated in serum. Whereas, non-protein sulfhydryl (NP-SH), total protein (TP) and malondialdehyde (MDA) were estimated in liver tissue as markers for oxidative stress. Histopathological assessment was also done on liver tissue. CCl4 induced liver poisoning in all treated animals was evident by elevated serum GOT, GPT, ALP, GGT and bilirubin levels. Induction of oxidative stress in the liver tissue by CCl4 was evidenced by a fall in the levels of NP-SH and TP; and an increased level of MDA concentration. EER administration for 10 days prevented the CCl4 induced hepatic injury and oxidative stress. Furthermore, the extract also reduced the pentobarbital-induced prolongation of sleeping time in mice. The ability of rocket extract to protect the liver toxicity in rats was further confirmed by histological findings in the liver tissue. In conclusion, it was observed that Eruca sativa L. extract protects the liver against CCl4 induced hepatic injury through its potent antioxidant activity in rats.

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