scholarly journals Appraisal of the Antioxidative Potential of Aloe Barbadensis M. on Alcohol-Induced Oxidative Stress

2019 ◽  
Vol 63 (3) ◽  
pp. 34-46 ◽  
Author(s):  
D. I. Akinloye ◽  
R. N. Ugbaja ◽  
O. A. Dosumu
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Distilled Water ◽  
Oxidative Potential ◽  
Aloe Barbadensis ◽  
Group A ◽  
Oxidative Effects

Abstract This investigation estimated the anti-oxidative potential of Aloe barbadensis gel extracts in rats against alcohol-induced oxidative stress. Thirty male albino rats (5 each per group) were included in the experiments. Group A (positive control) and B (negative control) were administered 4 mg.kg–1 body weight distilled water and 50 % alcohol respectively for 21 days. Groups C and D were administered 50 % alcohol for the first 14 days followed by co-administration of 125 mg and 250 mg.kg−1 body weight extract with alcohol respectively for the last 7 days. Groups E and F were administered distilled water for the first 14 days followed by co-administration of 125 and 250 mg.kg−1 body weight Aloe barbadensis gel extracts with distilled water respectively for the last 7 days. The administration of alcohol resulted in a significant (P < 0.05) decrease in the specific activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH) levels, while cholesterol (CHO), triglycerides (TAG), nitric oxide (NO) and malondialdehyde (MDA) concentrations were significantly increased when compared to the controls. Co-mobilization with Aloe barbadensis gel extracts for 7 days significantly reversed the deleterious effects of alcohol in the treated groups when compared to the alcohol group. This study indicated that Aloe barbadensis probably possesses anti-oxidative effects against alcohol–induced oxidative stress in rats.

scholarly journals Evaluation of Carrot Pomace (Daucus carota L.) as Hypocholesterolemic and Hypolipidemic Agent on Albino Rats

2013 ◽  
Vol 5 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Abd El-Moneim M.R. AFIFY ◽  
Ramy R.M ROMEILAH ◽  
Mahmoud M.H. OSFOR ◽  
Amir S.M. ELBAHNASAWY
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Obese People ◽  
Daucus Carota L ◽  
Hypercholesterolemic Diet

The current study examined the attenuating influence of dietary carrot pomace powder (CaPP) on hypercholesterolemia and various oxidative stress-associated with biochemical parameters in hypercholesterolemic rats. Thirty two male albino rats weighing 110±10 g were divided into four groups, the first group received the basal diet only and served as (negative control), the second group received the hypercholesterolemic diet and served as positive control, the other groups received hypercholesterolemic diet supplemented with 10%, 20% CaPP for six weeks. The obtained results revealed that groups supplemented with 10% and 20% CaPP significantly decrease total lipid, total cholesterol, triglycerides, low density lipoprotein cholesterol, liver enzymes: alanine aminotransferase, aspartate aminotransferase compared to positive and negative groups. Organs weight, body weight gain significantly decreased compared with positive control. Moreover dietary carrot pomace powder can used to reduce the body weight and reducing hypercholesterolemic complications. In addition, dietary carrot pomace powder serves to improve the blood picture and to reduce the blood glucose level in hypercholesterolemic rats and could use in obese people for body loss. Data of kidney function (Urea) record an increase in CaPP 20% level (26.9±2.96) but this increase was non significant with the negative control group (26.6±3.1).

scholarly journals Anti-Oxidant and Hepatoprotective Effects of Senecio biafrae on CCl4-induced Liver Damage in Rats

2019 ◽  
Vol 13 (2) ◽  
pp. 31-35
Author(s):  
Israel Oghenevwodoko Okoro ◽  
◽  
Helen Ejiro Kadiri ◽  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Root Extract ◽  
Distilled Water ◽  
Standard Drug ◽  
Anti Oxidant

Background: The present study was performed to explore whether the aqueous extract of Senecio biafrae (S. biafrae) roots provide any in vivo protective activity against carbon tetrachloride (CCl4)-induced hepatotoxicity in male albino rats. Methods: Rats (150-200 grams) were grouped into five groups (A-E) of six rats each and were treated orally for twelve days with 72 hourly administration of CCl4 (1 mL/kg) as follows: Group A received distilled water only (negative control), Group B was administered distilled water plus CCl4 (positive control), Group C was administered 400 mg/kg extract and CCl4, Group D received 200 mg/extract and CCl4, while Group E was administered standard drug (Silymarin 25mg/kg, PO). Results: Pre-treatment with the extract of S. biafrae (200 or 400mg/kg) or Silymarin (25mg/kg) caused significant restoration in the biomarkers as evaluated by reducing the levels of malondialdehyde, transaminases and elevating the levels of superoxide dismutase, catalase and glutathione peroxidase activities, which were altered by CCl4 toxicity. The extract at a dose of 400mg/kg demonstrated similar activities comparable to the standard drug (Silymarin). Conclusion: The results of this study indicate that the root extract of S. biafrae possesses hepatoprotective and anti-oxidant properties which may be due to the presence of phytochemicals in it.

scholarly journals To Evaluate the Diuretic Activity in Ethanolic Extract of Leaves of Delonix Regia in Wistar Albino Rats

2018 ◽  
Vol 11 (2) ◽  
pp. 959-963
Author(s):  
Jayanthi M K ◽  
Siddamma Amoghimath
Keyword(s):  
Body Weight ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Diuretic Activity ◽  
Delonix Regia ◽  
Group 4 ◽  
Group 2 ◽  
Wistar Albino Rats

To study the diuretic activity in ethanolic extract of leaves of delonix regia in wistar albino rats. After obtaining the permission from the Institutional Ethical Committee (IAEC) Wistar albino rats were randomly selected and divided into 4 groups. The animals were fasted and deprived of food and water 20hrs prior to the experiment. On the day of experimentation, Group 1 negative control received Normal saline (25ml/kg), Group 2 positive control received Furosemide 20mg/kg body weight, and Group 3 received ethanonic extract of delonix regia 100mg/kg and Group 4 received ethanonic extract of delonix regia 200 mg/kg. Delonix regia increase the excretion of sodium and water at the dose of 100mg/kg and 200mg/kg of body weight. The diuretic index is 5.93 and 6.45 at 100mg/kg and 200mg/kg of delonix regia respectively. The diuretic activity if delonix regia 100mg/kg is 0.61 and 0.67 at dose 200mg/kg, which is more than that of negative control but less when compared to positive control. Ethanonic extract of Delonix regia showed significant diuretic activity at both 100mg/kg and 200mg/kg doses.

scholarly journals Hepatoprotective Effects ofSilybum marianum(Silymarin) andGlycyrrhiza glabra(Glycyrrhizin) in Combination: A Possible Synergy

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mahmood Rasool ◽  
Javed Iqbal ◽  
Arif Malik ◽  
Hafiza Sobia Ramzan ◽  
Muhammad Saeed Qureshi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Injury ◽  
Liquid Paraffin ◽  
Thiobarbituric Acid ◽  
Positive Control ◽  
Albino Rats ◽  
Dosage Regimens ◽  
Group A ◽  
Hepatoprotective Effects ◽  
Different Doses

Oxidative stress, lipid peroxidation, and transaminase reactions are some of the mechanisms that can lead to liver dysfunction. A time-dependent study was designed to evaluate the ability of silymarin (SLN) and glycyrrhizin (GLN) in different dosage regimens to lessen oxidative stress in the rats with hepatic injury caused by the hepatotoxin carbon tetrachloride. Wistar male albino rats (n= 60) were randomly assigned to six groups. Group A served as a positive control while groups B, C, D, E, and F received a dose of CCl4(50% solution of CCl4in liquid paraffin, 2 mL/kg, intraperitoneally) twice a week to induce hepatic injury. Additionally, the animals received SLN and GLN in different doses for a period of six weeks. CCl4was found to induce hepatic injury by significantly increasing serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and thiobarbituric acid reactive substances while decreasing total protein and the activities of reduced glutathione, superoxide dismutase, and catalase. Treatment with various doses of SLN and GLN significantly reduced ALT, AST, ALP, and TBARS levels and increased GSH, SOD, and CAT levels. Our findings indicated that SLN and GLN have hepatoprotective effects against oxidative stress of the liver.

scholarly journals Effects of Aqueous Extract of Vigna unguiculata Seedlings Against Doxorubicin-Induced Cardiotoxicity and Kidney damage In Female Albino Rats

2016 ◽  
Vol 1 (04) ◽  
Author(s):  
Olatunde A. Oseni ◽  
Ademola S. K. Idowu ◽  
Aminat Isah
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Vigna Unguiculata ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Enzyme Markers

Doxorubicin is an anthracycline drug which is believed to cause immediate damage to myocardial cells by free radical generation in the cause of treatment of cancer. This study was however aimed to investigate the effects of the aqueous extract of the seedlings of Vigna unguiculata on heart, kidney as well as lipid profile disorders caused by this drug on female Wistar albino rats. Fifteen female Wistar albino rats were divided into three groups. Group 1 animals served as normal positive control; Group 2 animals served as negative control which were treated with 0.5mL of (20mg/kg body weight doxorubicin) while Group 3 animals were treated with 0.5mL each of 20mg/kg body weight doxorubicin and 10% aqueous extract of seedlings of Vigna unguiculata. Some enzyme markers and lipid contents were determined. The results of the study showed an increase in the activities of plasma ALP and AST after treatment with the drug except in the heart for AST which showed a significant reduction, while treatment with the extract brought about a decrease in the plasma and the organs except for kidney AST. The ALT on the other hand showed slight increase in the plasma with a decrease in the kidney and heart after treatment with the drug as the treatment with the extract tend to restore it to the control in both cases, there was increased plasma and kidney but reduced heart HDL-C after treatment with the drug which was observed to be restored to control after treatment with extract in both situations. The triglyceride and total cholesterol did not show similar trend in the plasma and the studied organs. The LDL-C was also observed to be increased in both plasma and organs after the treatment with the drug which later reduced significantly towards the control after the administration of the sprout extract. The present study has shown that the doxorubicin has damaging effects on both kidney and heart tissues while the sprouts extract produce a restoration to the normal in both organs.

scholarly journals ETHNOPHARMACOLOGICAL STUDY OF BRAIN OXIDATIVE STRESS IMPROVING POTENTIAL OF CURCUMIN IN INTOXICATED RATS

2021 ◽  
pp. 62-66
Author(s):  
FATEN IBRAHIM EL-SAYED
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Brain Tissue ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Stress Effect ◽  
Stress Effects ◽  
Stress Markers ◽  
Group A

Objective: The following study aimed to investigate the efficacy of curcumin at preventing amikacin neurotoxicity Methods: Twenty-four male Wister albino rats were randomly divided into four groups including-G (1): control group includes six rats, they were administered 0.5 ml of saline orally for 14 consecutive days. G (2): includes six rats; they were administered 200 mg/kg curcumin orally for 14 consecutive days. G (3): includes six rats, they were administered 300 mg/kg body weight/day of amikacin intraperitoneally for 14 consecutive days G (4): includes six rats, they were administered 200 mg/kg curcumin orally concurrently with 300 mg/kg body weight/day of amikacin. All animals were kept in the same conditions from feed, heat and humidity. Results: According to the result obtained after sacrification of all animals after the end of 14 d, Results revealed that amikacin at the dose rate of 300 mg/kg b. wt for 14 d induces significant changes in oxidative stress markers compared to the control group, a significant reduction in CAT. SOD. GSH (1.51±0.16, 77.00±0.73 and 84.06±4.42) respectively compared to control (3.63±0.11, 98.48±0.18 and 117.05±0.52) along with a significant increase in MDA activity (219.02±3.34) compared to control group (180.42±0.19), That indicate oxidative stress effect of it. On the beneficial side rats received amikacin 300 mg/kg B. wt I/p concurrently with 200 mg/kg b. wt curcumin for successive 14day result in a significant increase in CAT. SOD. GSH (2.23±0.09,92.00±0.26, 102.25±1.71) and decrease in MDA concentration (139.23±3.89) compared to amikacin treated group levels along with histopathological changes appear in brain tissue in the group treated with amikacin include nuclear pyknosis and degeneration in some neurons in the hippocampus, multiple focal eosinophilic plaque formation in the striatum also this results enhanced by activated caspase-3 expression in the brain tissue following amikacin administration. Conclusion: The present study proved that Oral administration of curcumin at the dose of 200 mg/kg for 14 d concurrently with amikacin significantly mitigates its neurotoxic and oxidative stress effects.

scholarly journals HEPATOPROTECTIVE ACIVITY OF THE ETHANOLIC EXTRACT OF DOG FRUIT RIND (Pithecellobium lobatum Benth.)

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
Ruqiah Ganda Putri Panjaitan ◽  
Ely Savitri ◽  
Titin Titin
Keyword(s):  
Body Weight ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Alanine Transaminase ◽  
Albino Rats ◽  
Aspartate Transaminase ◽  
Wistar Albino Rats ◽  
Fruit Rind

The present study was conducted to investigate the hepatoprotective activity of ethanolic extract of dog fruit rind (Pithecellobium lobatum benth.) against carbon tetrachloride (CCl4) induced hepatotoxicity in rats. Male Wistar albino rats were randomly divided into four groups and administered orally with 50 mg/200 g body weight of dog fruit rind extract (K1), 100 mg/200 g body weight (K2) of dog fruit rind extract, 5 mg/200 g body weight of silymarin (K3/positive control), and 0.4 mL/200 g body weight of distilled water (K4/negative control), for seven days The levels of alanine transaminase (ALT) of each K1; K2; K3; and K4 were 143.40±83.75 U/L, 94.80±93.77 U/L, 130.20±58.54 U/L and 147.25±107.97 U/L, respectively, while the aspartate transaminase (AST) levels were 304.20±128.67 U/L; 213.20±88.93 U/L; 333.00±128.31 U/L; and 239.25 ± 94.90 U/L, respectively (P>0.05). Group K2 showed better histological pattern than other groups with 60% of mild and 40% of moderate liver damage. Our findings revealed the hepatoprotective activity of the ethanolic extract of dog fruit rind.

scholarly journals Haematopoeitic potentials of methanolic extract of Tetrapleura Tetraptera pods in male Albino rats

2021 ◽  
pp. 73-77
Keyword(s):  
Body Weight ◽  
Plasma Protein ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Livestock Industry ◽  
Tetrapleura Tetraptera ◽  
Successful Induction

Anaemia constitutes a major challenge in the livestock industry worldwide. This study was carried out to determine the haematopoietic potential of methanol extract of Tetrapleura tetraptera (T. tetraptera) in male albino rats. Twenty five rats weighing between 147 and 166 g were used for the study. Anaemia was induced by daily removal of 2ml of blood per 100g body weight for ten days. The extraction was by cold maceration. Thereafter, the animals were administered with the extract for seven days. The rats were treated with graded doses of the extract, 50mg/kg, 100mg/kg and 200mg/kg for groups A, B and C respectively while groups D (negative control) and E (positive control) received 5 ml/kg of water. Results showed successful induction of anaemia. There was significant (p<0.05) increases in the erythrocytic parameters and plasma protein after administration of the methanoloic extract of T. tetraptera. The concentration dependent increase in the PCV, HBC, RBC, MCH and MCHC in the groups treated with the extract when compared with the untreated groups indicates haematopoietic potentials of T. Tetraptera.

UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

2020 ◽  
pp. 68-73
Author(s):  
Yuni Asri Mulatsih Agami ◽  
Eka Wisnu Kusuma
Keyword(s):  
Oxidative Stress ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Experimental Methods ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Negative Group ◽  
Ic50 Value ◽  
Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7%  Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7  setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif.    Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii  in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

The Impact of L-Carnitine and Coenzyme Q10 as Protection Against Busulfan-Oxidative Stress in the Liver of Adult Rats

2020 ◽  
Vol 10 (5) ◽  
pp. 578-586
Author(s):  
Areeg M. Abdelrazek ◽  
Shimaa A. Haredy
Keyword(s):  
Oxidative Stress ◽  
Coenzyme Q10 ◽  
Protective Role ◽  
Albino Rats ◽  
Distilled Water ◽  
Negative Effects ◽  
Adult Rats ◽  
Stress Damage ◽  
The Impact ◽  
Liver Tissues

Background: Busulfan (Bu) is an anticancer drug with a variety of adverse effects for cancer patients. Oxidative stress has been considered as a common pathological mechanism and it has a key role in the initiation and progression of liver injury by Bu. Aim: The study aimed to evaluate the antioxidant impact of L-Carnitine and Coenzyme Q10 and their protective role against oxidative stress damage in liver tissues. Methods and Material: Thirty-six albino rats were divided equally into six groups. G1 (con), received I.P. injection of DMSO plus 1 ml of distilled water daily by oral gavages; G2 (Bu), received I.P. injection of Bu plus 1 ml of the distilled water daily; G3 (L-Car), received 1 ml of L-Car orally; G4 (Bu + L-Car) received I.P. injection of Bu plus 1 ml of L-Car, G5 (CoQ10) 1 ml of CoQ10 daily; and G6 (Bu + CoQ10) received I.P. injection of Bu plus 1 ml of CoQ10 daily. Results: The recent data showed that Bu induced significant (P<0.05) elevation in serum ALT, AST, liver GSSG, NO, MDA and 8-OHDG, while showing significant (P<0.05) decrease in liver GSH and ATP. On the other hand, L-Carnitine and Coenzyme Q10 ameliorated the negative effects prompted by Bu. Immunohistochemical expression of caspase-3 in liver tissues reported pathological alterations in Bu group while also showed significant recovery in L-Car more than CoQ10. Conclusion: L-Car, as well as CoQ10, can enhance the hepatotoxic effects of Bu by promoting energy production in oxidative phosphorylation process and by scavenging the free radicals.

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