scholarly journals Antioxidant and Wound Healing Activity of Polyherbal Fractions ofClinacanthus nutansandElephantopus scaber

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Muhammad Shahzad Aslam ◽  
Muhammad Syarhabil Ahmad ◽  
Awang Soh Mamat ◽  
Muhammad Zamharir Ahmad ◽  
Faridah Salam
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Fraction ◽  
Burn Wound ◽  
Polyherbal Formulation ◽  
Wound Model ◽  
Elephantopus Scaber

Elephantopus scaberandClinacanthus nutansare traditionally used as wound healing herb. The objective of the present study is to develop a new polyherbal formulation, by comparison, the herbal combination ofElephantopus scaberandClinacanthus nutansas an in vitro antioxidant activity with their individual herbal activity followed by fractionation of polyherbal formulation for in vivo wound healing activities and identification of bioactive compounds from their active fractions. Antioxidant activity was performed in vitro by DPPH scavenging antioxidant activity followed by in vivo wound healing activities using excision wound model, incision wound model, and burn wound model. Toxicity of the fractions of the polyherbal formulation was performed by a dermal toxicity test. The result showed thatElephantopus scabercrude extract on the basis of EC50performs a much faster action (15.67 μg/mL) but with less % inhibition (87.66%) as compared to the combination of the new polyherbal formulation of crude extract (30 μg/mL). The polyherbal formulation has the highest % inhibition (89.49%) at the same dose as compared toElephantopus scaber(87.66%). In comparison among all crude and fractions of new polyherbal formulation, it was found that the ethyl acetate fraction of polyherbal formulation has the fastest activity (EC5014.83 μg/mL) with % inhibition (89.28%). Furthermore, during evaluation of wound contraction on excision and incision wound model, ethyl acetate fraction possesses the highest activity with (P< 0.001) and (P< 0.0001), respectively. During burn wound model, aqueous fraction (P< 0.001) possesses the highest activity followed by an ethyl acetate fraction (P< 0.0001). LC-MS analysis discovered the presence of several flavonoid-based compounds that work synergistically with sesquiterpene lactone and other bioactive compounds. In conclusion, flavonoid increases the antioxidant activity that surges the rate of wound contraction and works synergistically with other bioactive compounds.

scholarly journals In Vitro Antioxidant Activity and Phenolic Content of Cedrus brevifolia Bark

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Elena Cretu ◽  
Juha-Pekka Salminen ◽  
Maarit Karonen ◽  
Anca Miron ◽  
Christiana Charalambous ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Reducing Power ◽  
Ethyl Acetate Fraction ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Highly Correlated ◽  
Antioxidant Effects

A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50=13.9 ± 0.3 and 2.3 ± 0.0 μg/mL, respectively) and reducing capacity (EC50=9.1 ±0.1 μg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r=0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.

scholarly journals Identification of Active Compound Extracts from Noni Fruit (Morinda citrifolia L.) and Its Potential as Antioxidants

2019 ◽  
Vol 22 (5) ◽  
pp. 206-212
Author(s):  
Sogandi Sogandi ◽  
Rabima Rabima
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Morinda Citrifolia ◽  
Chloroform Fraction ◽  
Oxidation Reactions ◽  
First Time

Antioxidants are compounds that can inhibit oxidation reactions by binding to free radicals and highly reactive molecules. One form of reactive oxygen compounds is free radicals, these compounds are formed in the body and are triggered by various factors. Noni fruits have been used by the community as a medicine for high blood pressure, beriberi, urinating, inflammation of bile, inflammation of the intestine, dysentery, constipation, spleen pain, liver pain, diabetes and lumbago. Nevertheless, research on the identification of the active compounds of noni fruit which act as antioxidants yet. This study aims to extract and fractionation of Noni fruit, determine the antioxidant activity of Noni fruit extract, and identify the types of bioactive compounds that act as antioxidants. Noni fruit was extracted used maceration technique with 96% ethanol and then fractionated using n-hexane, chloroform and ethyl acetate solvents. Antioxidant activity from the results of noni fruit fractionation was measured by the DPPH (1.1 diphenyl-2-picrylhydrachyl) method and identification of bioactive compounds was carried out by GCMS. The results showed the greatest antioxidant activity was found in chloroform fraction with a percentage of inhibition of 78.19%. For the first time this study revealed the types of bioactive compounds from noni fruit ethyl acetate fraction which act as antioxidants are n-hexadecanoic acid, squalene, pyridin-3-carboxamide, oxime, n- (2-trifluoro methyl phenyl), and beta-sitosterol

scholarly journals Determination of total phenolic and flavonoid contents of jackfruit peel and in vitro antiradical test

Food Research ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 84-90
Author(s):  
Yamin ◽  
Ruslin ◽  
Mistriyani ◽  
Sabarudin ◽  
S. Ihsan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Colorimetric Method ◽  
Ethyl Acetate Fraction ◽  
Premature Aging ◽  
Natural Antioxidant ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Free radical is any molecular species that have unpaired free electrons in their outer orbital shell that make radicals highly reactive, resulting in pathogenesis conditions such as cellular injury, premature aging, cancer, hepatic disorders, neurodegenerative diseases, cardiovascular disease, and kidney disease. One source of natural antioxidant is jackfruit. The purpose of this research was to determine the phenolic and flavonoid contents in the extracts and fractions of jackfruit peel and their potential as antioxidants. Jackfruit peel powder was extracted from maceration. The total phenolic content was determined by the Folin-Ciocalteu method. Meanwhile, flavonoid content was determined using the aluminium chloride complex colorimetric method. Measurements of antioxidant activity were conducted using the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The ethyl acetate fraction had high phenolic and flavonoid contents, which were 49.667±1.508 g GAE/100 g of sample and 70.199±0.374 g of quercertin equivalent/100 g. The ethyl acetate fraction had the strongest antioxidant activity with IC50 value of 4.539±0.201 µg/ mL and correlation value (R2 ) of 0.5881 for phenols and R2 of 0.7241 for flavonoids. Ethyl acetate fraction of jackfruit peel is very potential to be developed as a natural antioxidant and functional food.

Chemical composition, antioxidant, alpha-glucosidase inhibitory, anticholinesterase and photoprotective activities of the aerial parts of Schinus molle L.

2020 ◽  
Vol 16 ◽  
Author(s):  
Mustapha M. Bouhenna ◽  
Chawki Bensouici ◽  
Latifa Khattabi ◽  
Farid Chebrouk ◽  
Nabil Mameri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Sun Protection ◽  
Antidiabetic Activity ◽  
Protection Factor ◽  
Schinus Molle ◽  
Rp Hplc ◽  
Sun Protection Factor

Background: Schinus molle L. is a medicinal and aromatic plant widely used in folk medicine commonly found in Algeria and Mediterranean region. In the present work, we investigated the in vitro antioxidant, anti-cholinesterase, αglucosidase inhibitory and photoprotective potentials of the Schinus molle L. plant’s extract/fractions. The metabolite profile of Schinus molle L. was analyzed using RP-HPLC and GC-MS. Methods: Antioxidant activity was assessed using different tests. Anticholinesterase activity was investigated by quantifying the acetylcholinesterase and butyrylcholinesterase inhibitory activities. Antidiabetic activity was investigated by αglucosidase inhibition test, whereas photoprotective activity was evaluated by sun protection factor (SPF) using spectrophotometry UV-Vis. The extracts were then submitted to RP-HPLC–PDA and GC–MS analysis. Results: Ethyl acetate and buthanol fractions with high contents of total phenolics and flavonoids exhibited the strongest antioxidant activity. Ethyl acetate extract exhibited considerable enzyme inhibition potential on acetylcholinesterase, butyrylcholinesterase and α-glucosidase with percentages of inhibition of 99.08 ± 0.79 %, 100 % and 98.80 ± 0.18 % respectively. Furthermore, the ethyl acetate fraction showed high photoprotective activity with the sun protection factor (SPF) value = 38,26 ± 0.73. Three phenolic acids (gallic, tannic and vanillic acids) and five flavonoids (myricetin, apigenin, naringenin, rutin and quercetin) were identified with RP-HPLC–PDA. Conclusion: These findings suggest that Schinus molle L. may be useful in the development of an alternative agent for oxidative stress, Alzheimer’s disease, diabetes and could be used as a natural sunscreen in pharmaceutics or cosmetic formulations.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF POLYHERBAL FORMULATION

2017 ◽  
Vol 9 (6) ◽  
pp. 12
Author(s):  
Madhuri A. Theng ◽  
G. R. Sitaphale ◽  
K. R. Biyani
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Soxhlet Extraction ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Butea Monosperma ◽  
Polyherbal Formulation ◽  
Wound Model ◽  
Acacia Arabica ◽  
Wound Healing Activity

Objective: The present study describes the anti-microbial acivity of Acacia arabica and Butea monosperma bark extract.Methods: For this purpose aqueous extract of bark were prepared by “Soxhlet extraction method”. The experimentally induced burn wound model in rats by “Excision method”.Results: As a result of this study it was found that the extract of bark generally revealed antimicrobial and wound healing activity.Conclusion: The result of the study suggest that the Acacia arabica and Butea monosperma bark of polyherbal gel effective in accelerating wound healing process.

scholarly journals in vitro Antioxidant Activity of Ethyl Acetate Fraction of Fermentation Products of Ginkgo biloba

2014 ◽  
Vol 26 (21) ◽  
pp. 7351-7354 ◽  
Author(s):  
Xuan Liu ◽  
Xin Zhan ◽  
Xianwei Zou ◽  
Yun Huang ◽  
Qian Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ginkgo Biloba ◽  
Ethyl Acetate Fraction ◽  
Fermentation Products ◽  
In Vitro Antioxidant Activity

scholarly journals Evaluation of the antioxidant potentials of extract and fractions of Vitex doniana fruit and identification of the bioactive metabolites using HPLC-DAD-MS analysis

2021 ◽  
Vol 17 (1) ◽  
pp. 160-175
Author(s):  
Keziah Uchechi Ajah ◽  
Ozioma Anne Asomugha ◽  
Chinazom Perpetua Ifedigbo ◽  
Kosoluchukwu Chidubem Umeh ◽  
Onyeka Chinwuba Obidiegwu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Vitex Doniana

Isoflavones and flavonoids in general found in fruits and vegetable act as natural antioxidants. This study was aimed at screening the isoflavone-rich fractions of Vitex doniana fruit extract for antioxidant activity using in vitro and in vivo models. The ethyl acetate and butanol fractions were screened for in vitro antioxidant activity by DPPH free radical scavenging and FRAP (Fe3+ Reducing Antioxidant Power) assays using ascorbic acid as standard. In vivo antioxidant activity was also tested against carbon tetrachloride-induced toxicity in mice using carbon tetrachloride (4 mL/kg) and silymarin (100 mg/kg) as standard. The ethyl acetate fraction and butanol Vacuum Liquid Chromatography (VLC) sub-fractions were subjected to High Performance Liquid Chromatography–Diode Array Detector (HPLC-DAD) and Liquid Chromatography-Mass Spectrometry (LC-MS) analyses. The ethyl acetate and butanol fractions inhibited DPPH radicals with an IC50 of 897 µg/mL and 2809.38 µg/mL respectively. Both fractions also showed mild FRAP result with EC50 of 1401 µg/mL and 7051 µg/mL respectively. The fractions produced significant decrease (P < 0.05) in ALP levels only while ethyl acetate fraction caused a significant reduction in the value of only malondialdehyde. Both fractions also increased levels of superoxide dismutase (SOD). HPLC-DAD analysis led to the detection of cinnamic acid, protocatechuic acid and two isoflavones daidzein and genistein. Further LC-MS analysis confirmed the presence of the isoflavones tectorigenin, 5-O-methylgenistein, and 5-O-methyltectorigenin, among other flavonoids, peonidin, 5-methylpeonidin, methylmalvidin and kaempferol-3-O-(2''galloyl)-glucopyranoside. The detected isoflavones and other flavonoids may contribute to the observed mild in vitro and good in vivo antioxidant activity of V. doniana fruit extract and fractions.

Antioxidant, anti-inflammatory and healing potential of ethyl acetate fraction of Bauhinia ungulata L. (Fabaceae) on in vitro and in vivo wound model

2020 ◽  
Vol 47 (4) ◽  
pp. 2845-2859
Author(s):  
Raphael de Oliveira Rodrigues ◽  
Juliana Navarro Ueda Yaochite ◽  
Greyce Luri Sasahara ◽  
Andressa Almeida Albuquerque ◽  
Said Gonçalves da Cruz Fonseca ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Wound Model ◽  
Anti Inflammatory

scholarly journals Antioxidant, Hepatoprotective, and Antidepression Effects of Rumex tingitanus Extracts and Identification of a Novel Bioactive Compound

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Dhekra Mhalla ◽  
Karama Zouari Bouassida ◽  
Rachid Chawech ◽  
Amira Bouaziz ◽  
Samar Makni ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Liver Toxicity ◽  
Bioactive Compound ◽  
Ethyl Acetate Fraction ◽  
Hydroalcoholic Extract ◽  
Immobility Time ◽  
Promising Source

Over the last few decades, Rumex species have been recognized as a promising source of new compounds with numerous pharmacological activities. Therefore, the antioxidant activity of Rumex tingitanus (R. tingitanus) leaves extracts was evaluated in vitro and then confirmed in vivo as well as the antidepressant-like and toxicological effects of the extracts. The ethyl acetate fraction (Rt EtOAcF) followed by hydroalcoholic extract (Rt EtOH-H2O) showed a remarkable in vitro antioxidant activity. The hydroalcoholic extract (Rt EtOH-H2O) showed significant hepatoprotective activity against carbon tetrachloride- (CCl4-) induced liver toxicity which is seen from inhibition of the malondialdehyde (MDA) accumulation and enhancement of the liver antioxidant enzymes activities. The Rt EtOH-H2O and Rt EtOAcF extracts were able to reduce the immobility time in mice and then elicited a significant antidepressant-like effect. The ethyl acetate fraction (Rt EtOAcF) was purified and resulted in the identification of a new antioxidant component called 4′-p-acetylcoumaroyl luteolin. The Rt EtOAcF and the 4′-p-acetylcoumaroyl luteolin revealed a strong antioxidant activity using DPPH test with IC50 of 11.7 ± 0.2 and 20.74 ± 0.6 μg/ml, respectively, and AAI of 3.39 and 1.92 better than that of BHT, used as control.

scholarly journals Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts

2019 ◽  
Vol 8 (2S7) ◽  
pp. 87-89
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Value ◽  
Dpph Method

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants

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