Component Release and Mechanical Properties of Endodontic Sealers following Incorporation of Antimicrobial Agents

Root canal sealers with antimicrobial activity are highly beneficial; therefore, their antimicrobial properties could be improved by incorporation of antimicrobial agents. In the present study, the release of the quaternary ammonium compounds from endodontic sealers admixed with either benzalkonium chloride (BC) or cetylpyridinium chloride (CPC) at loadings of 2% wt was monitored. The effect of these additives on the compressive strengths and their release from the sealers was determined after 1 and 4 weeks. All of the materials studied were found to be capable of releasing antimicrobial additive in useful quantities. The release of CPC occurred to a statistically significant greater extent than BC for all materials. The addition of both BC and CPC generally decreased the compressive strength of all the endodontic sealers, with the exception of CPC in AH Plus, where the compressive strength was significantly increased. This suggests that, for these endodontic sealers, the antimicrobial additives alter the setting chemistry. AH Plus is an epoxy-based material cured with an amine, and in this case the increase in compressive strength with CPC is attributed to an enhanced cure reaction with this system. In all other cases, the additive inhibited the cure reaction to a greater or lesser extent.

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Physiological and Transcriptional Characterization of Persistent and Nonpersistent Listeria monocytogenes Isolates

Applied and Environmental Microbiology ◽

10.1128/aem.05529-11 ◽

2011 ◽

Vol 77 (18) ◽

pp. 6559-6569 ◽

Cited By ~ 74

Author(s):

Edward M. Fox ◽

Nola Leonard ◽

Kieran Jordan

Keyword(s):

Listeria Monocytogenes ◽

Antimicrobial Agents ◽

Cetylpyridinium Chloride ◽

Phenotype Microarray ◽

Content Type ◽

Benzethonium Chloride ◽

Transcriptomic Sequencing ◽

Ammonium Compounds ◽

Compare Gene Expression

ABSTRACTThis study aimed to characterize physiological differences between persistent and presumed nonpersistentListeria monocytogenesstrains isolated at processing facilities and to investigate the molecular basis for this by transcriptomic sequencing. Full metabolic profiles of two strains, one persistent and one nonpersistent, were initially screened using Biolog's Phenotype MicroArray (PM) technology. Based on these results, in which major differences from selected antimicrobial agents were detected, another persistent strain and two nonpersistent strains were characterized using two antimicrobial PMs. Resistance to quaternary ammonium compounds (QACs) was shown to be higher among persistent strains. Growth of persistent and nonpersistent strains in various concentrations of the QACs benzethonium chloride (BZT) and cetylpyridinium chloride (CPC) was determined. Transcriptomic sequencing of a persistent and a presumed nonpersistent strain was performed to compare gene expression among these strains in the presence and absence of BZT. Two strains, designated “frequent persisters” because they were the most frequently isolated at the processing facility, showed overall higher resistance to QACs. Transcriptome analysis showed that BZT induced a complex peptidoglycan (PG) biosynthesis response, which may play a key role in BZT resistance. Comparison of persistent and nonpersistent strains indicated that transcription of many genes was upregulated among persistent strains. This included three gene operons:pdu,cob-cbi, andeut. These genes may play a role in the persistence ofL. monocytogenesoutside the human host.

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In vitro evaluation of the antimicrobial activity of five root canal sealers

Brazilian Dental Journal ◽

10.1590/s0103-64402004000100006 ◽

2004 ◽

Vol 15 (1) ◽

pp. 30-35 ◽

Cited By ~ 23

Author(s):

Brenda Paula Figueiredo de Almeida Gomes ◽

José Assis Pedroso ◽

Rogério Castilho Jacinto ◽

Morgana Eli Vianna ◽

Caio Cezar Randi Ferraz ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Contact Method ◽

Streptococcus Sanguis ◽

Actinomyces Naeslundii ◽

Agar Diffusion Test ◽

Significant Difference ◽

Ah Plus ◽

Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

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In VitroEvaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials againstEnterococcus faecalis,Candida albicans, andStaphylococcus aureus

International Journal of Biomaterials ◽

10.1155/2014/383756 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

Duddi Narendra Nirupama ◽

Mohan Thomas Nainan ◽

Rajendran Ramaswamy ◽

Sethumadhavan Muralidharan ◽

Hulimangala Hosakote Lingareddy Usha ◽

...

Keyword(s):

Antimicrobial Activity ◽

Direct Contact ◽

Microbial Growth ◽

Antimicrobial Property ◽

Recurrent Infection ◽

Antimicrobial Effect ◽

Ah Plus ◽

Endodontic Sealers ◽

Root Canal Sealers ◽

Direct Contact Test

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms,E. faecalis, C. albicans, andS. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity againstE. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity againstC. albicansandS. aureuscompared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity againstE. faecalis, C. albicans, andS. aureus.

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Biosynthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against studied microorganisms and its effect on compressive strength

10.21203/rs.3.rs-35758/v2 ◽

2020 ◽

Author(s):

Enas Tawfik Enan ◽

Amal A. Ashour ◽

Sakeenabi Basha ◽

Nayef H. Felemban ◽

sanaa M. F. gad El-Rab

Keyword(s):

Antimicrobial Activity ◽

Compressive Strength ◽

Antimicrobial Agents ◽

Glass Ionomer Cement ◽

Antimicrobial Properties ◽

Visible Spectrum ◽

Test System ◽

Glass Ionomer ◽

Group D ◽

Ionomer Cement

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of restorations failure. The advise for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents.Objectives: Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength (CS) of GIC was also evaluated.Material and methods: Biosynthesis of AgNPs were done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus). The distribution of biofilm was examined via scanning electron microscope. The CS of the tested material was measured using a Material Test System(MTS).Results: UV-Visible spectrum showed a peak of 429 nm. TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5-25.8 nm. The maximum inhibitory zone was recorded for group D against both tested bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused an insignificant effect on CS of GIC.Conclusion: TheAgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against studied microorganisms. The agents can be safely added with minimal effect on mechanical properties of the original cement.

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Periapical repair after root canal filling with different root canal sealers

Brazilian Dental Journal ◽

10.1590/s0103-64402009000500006 ◽

2009 ◽

Vol 20 (5) ◽

pp. 389-395 ◽

Cited By ~ 11

Author(s):

Mário Tanomaru-Filho ◽

Juliane Maria Guerreiro Tanomaru ◽

Mario Roberto Leonardo ◽

Lea Assed Bezerra da Silva

Keyword(s):

Root Canal ◽

Histopathological Analysis ◽

Sodium Hypochlorite Solution ◽

Mineralized Tissue ◽

Root Canal Filling ◽

Root Canals ◽

Ah Plus ◽

Hematoxylin And Eosin ◽

Endodontic Sealers ◽

Root Canal Sealers

The aim of this study was to evaluate periapical repair after root canal filling with different endodontic sealers. Sixty-four root canals from dog´s teeth were filled, divided into 4 groups (n=16). Root canals were instrumented with K-type files and irrigated with 1% sodium hypochlorite solution. Root canals were filled in the same session by active lateral condensation of the cones and sealers: Intrafill, AH Plus, Roeko Seal and Resilon/Epiphany System. After 90 days, the animals were euthanized and the tissues to be evaluated were processed and stained with hematoxylin and eosin. For histopathological analysis, the following parameters were evaluated: inflammatory process, mineralized tissue resorption, and apical mineralized tissue deposition. Histopathological analysis demonstrated that Intrafill had less favorable results in terms of apical and periapical repair, compared to the other sealers (p<0.05). AH Plus, Roeko Seal, and Epiphany sealers had similar and satisfactory results (p>0.05). In conclusion, AH Plus and the materials Roeko Seal and Epiphany are good options for clinical use in Endodontics.

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SYNTHETIC ANTIMICROBIAL AGENT AND ANTIMICROBIAL FABRICS: PROGRESS AND CHALLENGES

IIUM Engineering Journal ◽

10.31436/iiumej.v19i2.929 ◽

2018 ◽

Vol 19 (2) ◽

pp. 10-29 ◽

Cited By ~ 1

Author(s):

Norashikin Binti Mat Zain ◽

John Olabode Akindoyo ◽

Mohammad Dalour Hossen Beg

Keyword(s):

Antimicrobial Agents ◽

Antimicrobial Properties ◽

Antimicrobial Treatment ◽

Environmentally Benign ◽

Active Lifestyle ◽

Polyhexamethylene Biguanide ◽

Safety And Health ◽

Future Challenges ◽

Plant Sources ◽

Ammonium Compounds

ABSTRACT: Recently, there is a strengthening requirement for antimicrobial fabrics that are resistant to pathogens, offer greater hygiene, and are protective in an active lifestyle. Synthetic compounds have been applied to impart antimicrobial properties to the fabrics. In this paper, synthetic antimicrobial agents, namely quaternary ammonium compounds (QACs), polyhexamethylene biguanide (PHMB), triclosan, and N-halamine are discussed along with their mechanisms of action. Research has been done on synthetic antimicrobial treatment of fabrics; the tested microorganisms used to determine their efficiency and durability are summarized. It is also found that although synthetic antimicrobial agents have excellent strength to kill the microorganisms, some of these antimicrobial agents are harmful and have adverse effects on the environment, safety, and health. Identification of these problems will provide future challenges to overcome and improve the techniques used for antimicrobial treatment of the fabrics. Based on the infromation presented herein, it is recommended that subsequent studies should consider the use of antimicrobial agents that are either obtained from plant sources or from microorganisms. On the other hand, nanotechnlogy should be explored for production of antimicrobial agents in further studies based on its potential to facilitate the production of environmentally benign antimicrobial agents. ABSTRAK: Kebelakangan ini, permintaan terhadap fabrik antimikrobial bagi mencegah jangkitan patogen, penjagaan kebersihan dan perlindungan bagi gaya hidup yang aktif semakin meningkat. Sebatian sintetik telah diguna pakai bagi menambah sifat antimikrobial pada fabrik. Di dalam artikel ini, agen antimikrob sintetik yang dinamakan sebagai sebatian ammonium kuater (QAC), polyhexamethylene biguanide (PHMB), triclosan dan N-halamine serta mekanisma tindak balasnya telah dibincangkan. Penyelidikan telah dijalankan terhadap fabrik yang dirawat menggunakan agen antimikrob sintetik. Mikroorganisma yang digunakan bagi menentukan kecekapan dan daya tahan turut diringkaskan. Keputusan menunjukkan agen antimikrobial sintetik mempunyai tindak balas yang sangat baik untuk membunuh mikroorganisma, namun sebahagian agen antimikrobial ini berbahaya dan mempunyai kesan buruk terhadap alam sekitar, keselamatan dan kesihatan. Melalui kaedah kenal pasti masalah, ia dapat memberi solusi dan membaiki teknik rawatan antimikrobial pada fabrik pada masa hadapan. Hasil keputusan dan perbincangan menunjukkan kajian seterusnya harus menimbang penggunaan agen antimikrobial yang diperoleh dari sumber tumbuhan. Selain itu, nanoteknologi harus diterokai bagi pengeluaran agen antimikrobial berdasarkan potensinya yang mesra alam.

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Green synthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against dental biofilm and its effect on compressive strength

10.21203/rs.3.rs-35758/v1 ◽

2020 ◽

Author(s):

sanaa M. F. gad El-Rab ◽

Enas Tawfik Enan ◽

Amal A. Ashour ◽

Sakeenabi Basha ◽

Nayef H. Felemban

Keyword(s):

Antimicrobial Activity ◽

Compressive Strength ◽

Antimicrobial Agents ◽

Glass Ionomer Cement ◽

Antimicrobial Properties ◽

Test System ◽

Glass Ionomer ◽

Dental Biofilm ◽

Group D ◽

Ionomer Cement

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of their failure. The urge for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents. Objectives Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength of GIC was also evaluated. Material and methods Biosynthesis of AgNPs was done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity. The distribution of biofilm was examined via scanning electron microscope. Compressive strength (CS) of the tested material was measured using a Material Test System (MTS). Results UV-Visible spectrum showed a peak of 429 nm.TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5–25.8 nm. The maximum inhibitory zone was recorded for group D against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused insignificant effect on compressive strength of GIC. Conclusion Cupressus macrocarpa extract biosynthesized AgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against dental biofilm. The agents can be safely added without impairing mechanical properties of the set cement.

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Antimicrobial properties of actively purified secondary metabolites isolated from different marine organisms

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200730144536 ◽

2020 ◽

Vol 21 ◽

Author(s):

Nilushi Indika Bamunu Arachchige ◽

Fazlurrahman Khan ◽

Young-Mog Kim

Keyword(s):

Secondary Metabolites ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Bacterial Species ◽

Antimicrobial Properties ◽

Antimicrobial Effect ◽

Cost Effective ◽

Resistance Mechanisms ◽

Marine Organisms ◽

Antimicrobial Compounds

Background: The treatment of infection caused by pathogenic bacteria becomes one of the serious concerns globally. The failure in the treatment was found due to the exhibition of multiple resistance mechanisms against the antimicrobial agents. Emergence of resistant bacterial species has also been observed due to prolong treatment using conventional antibiotics. To combat these problems, several alternative strategies have been employed using biological and chemically synthesized compounds as antibacterial agents. Marine organisms considered as one of the potential sources for the isolation of bioactive compounds due to the easily available, cost-effective, and eco-friendly. Methods: The online search methodology was adapted for the collection of information related to the antimicrobial properties of marine-derived compounds. These compound has been isolated and purified by different purification techniques, and their structure also characterized. Furthermore, the antibacterial activities have been reported by using broth microdilution as well as disc diffusion assays. Results: The present review paper describes the antimicrobial effect of diverse secondary metabolites which are isolated and purified from the different marine organisms. The structural elucidation of each secondary metabolite has also been done in the present paper, which will help for the in silico designing of the novel and potent antimicrobial compounds. Conclusion: A thorough literature search has been made and summarizes the list of antimicrobial compounds that are isolated from both prokaryotic and eukaryotic marine organisms. The information obtained from the present paper will be helpful for the application of marine compounds as antimicrobial agents against different antibiotic-resistant human pathogenic bacteria.

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Sputter-Deposited Ag Nanoparticles on Electrospun PCL Scaffolds: Morphology, Wettability and Antibacterial Activity

Coatings ◽

10.3390/coatings11030345 ◽

2021 ◽

Vol 11 (3) ◽

pp. 345

Author(s):

Daniele Valerini ◽

Loredana Tammaro ◽

Roberta Vitali ◽

Gloria Guillot ◽

Antonio Rinaldi

Keyword(s):

Ag Nanoparticles ◽

Food Packaging ◽

Antimicrobial Agents ◽

Antimicrobial Properties ◽

Deposition Process ◽

Polymer Fibers ◽

Antibacterial Action ◽

Porous Scaffolds ◽

Electrospinning Technique ◽

Sputtering Deposition

Porous scaffolds made of biocompatible and environmental-friendly polymer fibers with diameters in the nano/micro range can find applications in a wide variety of sectors, spanning from the biomedical field to textiles and so on. Their development has received a boost in the last decades thanks to advances in the production methods, such as the electrospinning technique. Conferring antimicrobial properties to these fibrous structures is a primary requirement for many of their applications, but the addition of antimicrobial agents by wet methods can present a series of drawbacks. In this work, strong antibacterial action is successfully provided to electrospun polycaprolactone (PCL) scaffolds by silver (Ag) addition through a simple and flexible way, namely the sputtering deposition of silver onto the PCL fibers. SEM-EDS analyses demonstrate that the polymer fibers get coated by Ag nanoparticles without undergoing any alteration of their morphological integrity upon the deposition process. The influence on wettability is evaluated with polar (water) and non-polar (diiodomethane) liquids, evidencing that this coating method allows preserving the hydrophobic character of the PCL polymer. Excellent antibacterial action (reduction > 99.995% in 4 h) is demonstrated against Escherichia coli. The easy fabrication of these PCL-Ag mats can be applicable to the production of biomedical devices, bioremediation and antifouling systems in filtration, personal protective equipment (PPE), food packaging materials, etc.

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Phenotypic and Genotypic Properties of Fluoroquinolone-Resistant, qnr-Carrying Escherichia coli Isolated from the German Food Chain in 2017

Microorganisms ◽

10.3390/microorganisms9061308 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1308

Author(s):

Katharina Juraschek ◽

Carlus Deneke ◽

Silvia Schmoger ◽

Mirjam Grobbel ◽

Burkhard Malorny ◽

...

Keyword(s):

Antimicrobial Agents ◽

Point Mutations ◽

Beta Lactamase ◽

Resistance Development ◽

Extended Spectrum Beta Lactamase ◽

E Coli ◽

German Food ◽

Sequence Types ◽

Ammonium Compounds ◽

Additional Point

Fluoroquinolones are the highest priority, critically important antimicrobial agents. Resistance development can occur via different mechanisms, with plasmid-mediated quinolone resistance (PMQR) being prevalent in the livestock and food area. Especially, qnr genes, commonly located on mobile genetic elements, are major drivers for the spread of resistance determinants against fluoroquinolones. We investigated the prevalence and characteristics of qnr-positive Escherichia (E.) coli obtained from different monitoring programs in Germany in 2017. Furthermore, we aimed to evaluate commonalities of qnr-carrying plasmids in E. coli. We found qnr to be broadly spread over different livestock and food matrices, and to be present in various sequence types. The qnr-positive isolates were predominantly detected within selectively isolated ESBL (extended spectrum beta-lactamase)-producing E. coli, leading to a frequent association with other resistance genes, especially cephalosporin determinants. Furthermore, we found that qnr correlates with the presence of genes involved in resistance development against quaternary ammonium compounds (qac). The detection of additional point mutations in many isolates within the chromosomal QRDR region led to even higher MIC values against fluoroquinolones for the investigated E. coli. All of these attributes should be carefully taken into account in the risk assessment of qnr-carrying E. coli from livestock and food.

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