scholarly journals Potent Phosphodiesterase Inhibition and Nitric Oxide Release Stimulation of Anti-Impotence Thai Medicinal Plants from “MANOSROI III” Database

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Aranya Manosroi ◽  
Theeraphong Tangjai ◽  
Charinya Chankhampan ◽  
Worapaka Manosroi ◽  
Yaravee Najarut ◽  
...  
Keyword(s):  
Sexual Behaviors ◽  
Oral Toxicity ◽  
Traditional Use ◽  
Standard Drug ◽  
Nitric Oxide Release ◽  
Plumbago Indica ◽  
Intromission Frequency ◽  
Ea.Hy926 Cells ◽  
Thai Medicinal Plants ◽  
Pde Inhibition

Seven plants in the top rank were selected from the “MANOSROI III” database using the two Thai keywords which meant impotence and sexual tonic. Boesenbergia rotunda (L.) Mansf. extract [EDP1-001(1)] gave the highest PDE inhibition activity of 4.36-fold sildenafil, a standard anti-impotence drug. Plumbago indica Linn. extract [EDP2-001(1)] exhibited the highest NO release stimulation activity of 666.85% which was 1.50-fold acetylcholine, a standard drug. Most selected plant extracts were nontoxic to EA.hy926 cells at 1.0 mg/mL. EDP1-001(1) exhibited the LD50 value of acute oral toxicity in male ICR mice of over 5,000 mg/kg body weight. EDP1-001(1) also indicated the improvement of sexual behaviors in the paroxetine-induced sexual dysfunction male mice with the evaluation of number of courtships (NC), mount frequency (MF), intromission frequency (IF), and ejaculatory frequency (EF) at 87.67±6.17, 121.00±23.50, 36.00±3.21, and 13.67±2.96 which were 2.63-, 1.27-, 0.53-, and 0.62-fold sildenafil-treated mice at day 14 of the treatments, respectively. The present study has not only confirmed the traditional use of Thai plants for the treatment of ED but also indicated the potential and application of the “MANOSROI III” database for Thai plant selection to be developed as ED food supplements.

scholarly journals Effects of Cnidium monnieri (L.) Cuss. fruit extract on sexual behaviors in male rats

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Thuy Duong Dau ◽  
Minh Ha Le ◽  
Tran Thi Giang Huong Nguyen ◽  
Thi Ngoc Mai Dang ◽  
Thi Nguyet Que Do ◽  
...  
Keyword(s):  
Sexual Behaviors ◽  
Hplc Method ◽  
Female Rats ◽  
Male Rats ◽  
Sexual Disorders ◽  
Fruit Extract ◽  
Traditional Use ◽  
Intromission Latency ◽  
Long Time ◽  
Intromission Frequency

Abstract Background Cnidium monnieri (L.) Cuss. has been used for a long time according to the traditional experiences and documentation in order to treat male sexual disorders. Osthole is one of the main bioactive components in Cnidium monnieri (L.) Cuss. In this study, we aimed to evaluate the effect of Cnidium monnieri (L.) Cuss. fruit extract of which osthole content was 35% (determined by HPLC method) on sexual behaviors in adult male rats. Methods Cnidium monnieri (L.) Cuss. fruit extract was administered orally at the dose of 150 mg/kg b.w and after 30 min, male rats were coupled with receptive adult female rats to evaluate sexual behaviors, including mounting and intromission. Results Cnidium monnieri (L.) Cuss. fruit extract at the dose of 150 mg/kg b.w increased the percentage of rats that mounted and intromitted; increased the intromission frequency and decreased mounting latency and intromission latency. Conclusion Cnidium monnieri (L.) Cuss. fruit extract increased the ability to mount and intromit in rats, and scientifically support the traditional use of Cnidium monnieri (L.) Cuss. in treatment of male sexual disorders.

scholarly journals Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ramesh C ◽  
Pinkey Rawal ◽  
Soma Pramanik ◽  
Shabana S
Keyword(s):  
Biochemical Parameters ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

Evaluation of Anti-Fertility Property of Lawsonia Inermis L. (Lythraceae) Roots in Rats

2015 ◽  
Vol 19 ◽  
pp. 65-70
Author(s):  
CT Samagoro ◽  
A Agunu ◽  
H Nuhu ◽  
Z Mohammed ◽  
EM Abdurrahman ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Ethinyl Estradiol ◽  
Corpora Lutea ◽  
Lawsonia Inermis ◽  
Control Groups ◽  
Traditional Use ◽  
Standard Drug ◽  
Male Wistar Rats ◽  
Implantation Sites ◽  
And Control

Traditional contraceptive practices and use of medicinal plants is a common occurrence in Kaduna State, Nigeria. Lawsonia inermis L. (Lythraceae) roots which is the most frequently used anti-fertility agent was evaluated in rats. Apparently healthy female and male Wistar rats were used. Pre-implantation and mating ratio, determinations of implantation sites and Corpora lutea were also conducted. The effects of extract on weight of the rats were also determined. There were loss of implantation sites and decrease in body weight. The number of implantation sites showed dose–response relationship significantly (p£0.05) among the doses of extract and to standard drug (ethinyl estradiol). There was also significant (p£0.05) difference observed in the number of Corpora lutea in all experimental and control groups. Similarly, there was significant (p£0.05) difference observed in all the experimental and control groups on percentage pre-implantation loss. The plant extract exhibit anti-fertility effects. The finding may explain the traditional use of the plant as antifertility agent.Keywords: Traditional Medicine, Contraception, Methanol Extract, L. inermis Root, Medicinal Plants

Hypoglycemic Activity of Methanol Fraction of Tectona grandis (Linn) Bark in Experimental Rat Models

2021 ◽  
pp. 4247-4252
Author(s):  
Bishwanath Mishra ◽  
Durga M. Kar ◽  
Laxmidhar Maharana ◽  
Sujit Dash ◽  
Ganesh P. Mishra
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Tectona Grandis ◽  
Hypoglycemic Activity ◽  
Oral Glucose ◽  
Alcoholic Extract ◽  
Traditional Use ◽  
Standard Drug ◽  
Single Dose Study ◽  
Dose Study

Diabetes mellitus (DM) is a now a major global health problem and its incidence is increasing day by day in whole world. There are various medicinal plants in India those possess antidiabetic property which are traditionally used in management of diabetes. Tectona grandis Linn. (TG) plant belonging to family Verbenaceae is medicinally reported and claims to cure various diseases in Indian traditional system of medicine (Ayurveda) and also in folklore. The purpose of this present study is to examine the hypoglycemic potential of methanol fractions (50, 100 and 200mg/kg body weight) of Tectona grandis bark (MFTG) from defatted hydro-alcoholic extract in normoglycemic, streptozotocin induced (45mg.kg‒1) diabetic and glucose loaded hyperglycemic rats by single and multiple oral administration in comparison to standard drug Glibenclamide (2.5 mg/kg body weight). Initially acute oral toxicity study of MFTG was carried out in rats to estimate the dose for animal study. The study report showed that the MFTG (200mg/kg) significantly (p<0.05 to p<0.01) reduces blood glucose level both in normoglycemic and diabetic rats induced by Streptozotocin and oral glucose loaded methods till the end of 8 hour and 3hour respectively during the single dose study and from the 15th day to 30th day in multi dose study. Hence the present study reveals that MFTG possess significant hypoglycemic activity which inspires the traditional use of the plant for the treatment of diabetes mellitus.

Sexual stimulant effects of the mixture of Mondia whitei, Dracaena arborea, and Bridelia ferruginea in normal and prediabetic male Wistar rats

2019 ◽  
Vol 30 (4) ◽  
Author(s):  
Pierre Watcho ◽  
Marc-Aurèle Tchuenchie Gatchueng ◽  
Patrick Brice Defo Deeh ◽  
Modeste Wankeu-Nya ◽  
Esther Ngadjui ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Wistar Rats ◽  
Tap Water ◽  
Sildenafil Citrate ◽  
Control Group ◽  
Traditional Use ◽  
Bridelia Ferruginea ◽  
Male Wistar Rats ◽  
Intromission Frequency ◽  
Performance Enhancers

Abstract Background We investigated the effects of a polyherbal formulation prepared from the extracts of Mondia whitei (Periplocaceae), Dracaena arborea (Dracaenaceae), and Bridelia ferruginea (Euphorbiaceae) (MDB) on the sexual behavior of normal rats (NR) and prediabetic rats (PR). Methods Male Wistar rats were administered with drinking fructose solution (21%) or tap water for 16 weeks. After induction of prediabetic status, NR (n = 30) and PR (n = 30) were randomly distributed into 10 groups of six animals each and orally treated with distilled water (10 mL/kg), sildenafil citrate (5 mg/kg), or MDB (50, 100, or 500 mg/kg) for 21 days. Sexual behavior parameters per series (S) of ejaculation were evaluated on days 1, 7, 14, and 21. Results Drinking fructose solution (21%) induced prediabetic status in rats, characterized by a significant (p < 0.01) increase in glycemia by 43.41% compared with the control group. MDB improved sexual performances of NR and PR by increasing the mount frequency (MF) and the intromission frequency (IF) as well as the number of rats capable of ejaculating. For instance, the MF and the IF were significantly increased in animals administered with MDB for 7 (50 mg/kg, S1 and S2), 14 (100 mg/kg, S3), or 21 days (100 or 500 mg/kg, S2). This increase was more pronounced on days 7 and 14 in NR and PR treated with sildenafil citrate or MDB extracts, respectively. Conclusions The mixture of MDB improved sexual activity in NR and PR. This result may further justify the traditional use of these plants as sexual performance enhancers.

scholarly journals Investigation of Antimicrobial Properties of Doscorea pentaphylla from Mid Western Ghats, India

1970 ◽  
Vol 8 (8) ◽  
pp. 91-96 ◽  
Author(s):  
G Prakash ◽  
BB Hosetti
Keyword(s):  
Western Ghats ◽  
Fungal Infections ◽  
Petroleum Ether Extract ◽  
Pathogenic Fungi ◽  
Antibacterial Drug ◽  
Bacterial Strains ◽  
Traditional Use ◽  
Standard Drug ◽  
Clinical Strains ◽  
Crude Extracts

Antibacterial and antifungal activity of crude extracts of medicinally important and traditionally used yam plant Dioscorea pentaphylla from mid Western Ghats was evaluated against 27 bacterial and 5 fungal clinical strains collected of the patients from infectious sources. The clinical strains belonging to their respective species showed concentration dependent susceptibility towards crude petroleum ether extract, chloroform extract and methanol extract at 100ìg/100ìL. All the extracts exhibited predominant antibacterial activity against S. aureus (ATCC-20852), P. aeruginosa (ATCC-29737) and K. pneumoniae (MTCC-618) respectively. and five clinically isolated pathogenic fungi, T. rubrum, M. gypseum, T. tonsurans, M. audouini, and C. albicans with antibacterial drug Ciprofloxacin and antifungal drug Fluconozole (50ìg/100ìL) as the standard drug. Out of three extracts, ethanol extracts possessed better minimum inhibition concentration against all the bacterial strains. All the three extracts showed significant result against all the five fungal pathogen strains. The results are promising and supported the traditional use of D. Pentaphylla for the treatment of bacterial and fungal infections. Keywords : Antifungal; Antibacterial; Crude extracts Dioscorea pentaphylla; MIC and Traditional medicine. DOI: 10.3126/sw.v8i8.3857 Scientific World Vol.8(8) 2010 pp.91-96

scholarly journals CHEMICAL ANALYSIS, ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS OF ACETONE EXTRACT OF ALGERIAN SOLENOSTEMMA ARGEL (DELILE) HAYNE LEAVES

2020 ◽  
pp. 72-81
Author(s):  
DAOUIA KELTOUM BENMAAROUF ◽  
DIANA C. G. A. PINTO ◽  
BERNARD CHINA ◽  
SAFIA ZENIA ◽  
KHEIRA BOUZID BENDESARI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Granuloma Formation ◽  
Paw Edema ◽  
Oral Toxicity ◽  
Traditional Use ◽  
Anti Inflammatory ◽  
Dose Dependent

Objective: To investigate the qualitative composition of the acetonic extract from leaves of S. argel (AESA) and their anti-inflammatory and analgesic properties in vivo. Methods: AESA profile was established by UHPLC/DAD/ESI-MS2. AESA was subjected to the acute oral toxicity study according to the OECD-420 method. Antioxydant activity of AESA was performed by DPPH radical scavenging assay. Anti-inflammatory effects of AESA were determined in two animal models: carrageenan-induced paw edema in rats and cotton pellet-induced granuloma formation in rats. Further, anti-nociceptives activities of AESA were assessed by hot plate test, acetic acid-induced abdominal writhing test and formalin test. Results: The in vivo AESA toxicity was low. AESA expresses a maximum radical scavenging activity with a IC50 value of 36,05 μg/ml. The AESA at 250 and 400 mg/kg significantly reduced carrageen an induced paw edema by 70.09% and 85.53% 6h after carrageenan injection, respectively. AESA produced significant dose-dependent anti-inflammatory effect against cotton pellets-induced granuloma formation in rats. In addition, AESA at 250 and 400 mg/kg significantly reduced acetic acid-induced writhing by 56.83 and 80.41%, respectively. Oral administration of 250 and 400 mg/kg of AESA caused a significant dose dependent anti-nociceptive effect in both neurogenic and inflammatory phases of formalin-induced licking. AESA also impacted the pain latency in the hot plat test. Conclusion: These data suggest that AESA possesses antioxidant, anti-inflammatory and anti-nociceptive effects. These results support the traditional use of S. argel to cure pain and inflammatory diseases in the Algerian Sahara.

scholarly journals EX-VIVO: ANTHELMINTIC EVALUATION OF BOMBAX CEIBA STEM EXTRACTS

2020 ◽  
pp. 431-436
Author(s):  
Sukhbir Lal Khokra ◽  
Sonia Gogat ◽  
Rakesh Narang ◽  
Manish Devgun
Keyword(s):  
Ex Vivo ◽  
Research Work ◽  
Anthelmintic Activity ◽  
Cost Effective ◽  
Stem Bark ◽  
Maximum Activity ◽  
Traditional Use ◽  
Standard Drug ◽  
Methanolic Extracts ◽  
And Control

Bombax ceiba plant belongs to bombacaceae family and is commonly known as Semal (Hindi) and Cotton tree (English). It has numerous effects like stimulant, astringent, haemostatic, diuretics, antidiarrhoeal, emeticand antipyretic. Major pharmacological activities reported in this plant are hypoglycemic, antianxiety, antibacterial, antiviral and anti-inflammatory. Numerous side effects and increased resistance to anthelmintic drugs prompted us to discover cost effective safe formulation of conventional medicinal plants. In the present study, research work is focused to evaluate the possible anthelmintic effects of Bombax ceiba. To investigate anthelmintic potential of Bombax ceiba plant, different extracts from dried powder stem bark were tested on adult Indian earthworms (Pheretima posthuma) having physiological & anatomical resemblance with human intestinal roundworm parasites. The drugs were divided in 14 groups consisting of test, standard and control. Each group was consisting of six Indian earthworms of approximately same size. Albendazole was used as standard drug while tween80 in normal saline (1:1) was used as control. The result data reveals that all extracts have significant anthelmintic activity as compared to Albendazole taken as standard drug. The methanolic extract at dose of 50 mg/ml concentration showed paralysis time 30±0.96 minutes and death 33.66±0.71 minutes, which is equivalent to standard drug values at dose of 20 mg/ml. Ethylacetate extract at dose of 50 mg/ml showed paralysis at 28.66±0.71 minutes and death at 34.16±1.10 minutes, which proves that it is even better than standard drug in terms of paralysis time and equipotent in terms of time taken for causing death. Thus among the extracts, ethylacetate and methanolic extracts showed maximum activity with least time taken for paralysis and death of earthworms and were found equally potent as Albendazole at a dose of 50 mg/ml. Thus, Bombax ceiba traditional use in helminitic infestation was proved scientifically. On comparing phytochemical evaluation with anthelmintic activity it is assumed that glycosides may be responsible for this activity. A further investigation may lead development of novel anthelmintic drug or formulation with anthelmintic activity.

scholarly journals Antimalarial Activity of Meriandra dianthera Leaf Extracts in Plasmodium berghei-Infected Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Kalay Hagazy ◽  
Gereziher G. Sibhat ◽  
Aman Karim ◽  
Gebretsadkan H. Tekulu ◽  
Gomathi Periasamy ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Mice Model ◽  
Oral Toxicity ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Crude Leaf Extract

Objective. To evaluate the antimalarial effect of aqueous methanolic extract and solvent fractions of Meriandra dianthera leaves against Plasmodium berghei in mice model. Method. M. dianthera leaves were extracted with 80% methanol and dried. The dried crude extract was then defatted and further fractionated with chloroform, ethyl acetate, and butanol. Acute oral toxicity test was performed as per the Organization for Economic Cooperation and Development guideline 425. Peter’s 4-day suppressive test was used to determine the in vivo antimalarial activity of the extract and fractions. Result. The crude leaf extract of Meriandra dianthera showed parasite inhibition of 42.28% and 45.52% at doses of 400 and 600 mg/kg, respectively, as compared to the negative control. Moreover, the mice which received chloroform and aqueous fractions at the dose of 400 mg/kg/day showed significant (P<0.001) chemosuppression compared to the negative control. Both the extract and fractions were able to prevent P. berghei induced body weight loss and body temperature reduction and also increased the survival time of the mice as compared to the negative control. The aqueous methanolic leaf extract of M. dianthera showed no gross signs of toxicity or mortality in mice until a single oral dose of 2000 mg/kg. Conclusion. The extracts of M. dianthera leaves showed promising antimalarial activity, with no sign of toxicity and therefore may support its traditional use for the treatment of malaria.

scholarly journals Evaluation of the antimicrobial activity and safety of Rhus vulgaris (Anacardiaceae) extracts

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Angela Mutuku ◽  
Lizzy Mwamburi ◽  
Lucia Keter ◽  
Joyce Ondicho ◽  
Richard Korir ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Skin Irritation ◽  
Toxicity Testing ◽  
Oral Toxicity ◽  
Traditional Use ◽  
One Way Anova

Abstract Background Medicinal plants have been used in the treatment of various ailments in most developing countries. Oral infections are the most prevalent diseases in man. The Rhus family has been found to have antimicrobial, antimalarial, and anti-inflammatory properties. Few studies have been done on Rhus vulgaris Meikle. A study was conducted to determine the effect of Rhus vulgaris Meikle stem bark extracts against selected oral pathogenic microorganisms and the safety of the extracts in vitro and in vivo. Methods Methanol:dichloromethane (1:1), methanol and aqueous extracts were tested for bacteriostatic and bactericidal effects against Methicillin Resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Streptococcus mutans and Candida albicans. Cytotoxicity of the active extracts was determined using Vero E6 cell lines while safety was evaluated in mice and rats. Phytochemical screening was performed on the methanol extracts. One-way ANOVA and Tukey’s multiple comparisons tests were performed using IBM SPSS statistics 20.0 for antimicrobial assay and acute toxicity testing. One-way ANOVA and Dunnett’s multiple comparison tests were conducted using GraphPad Prism 8.0 for cytotoxicity assay. Results Methanol extract of Rhus vulgaris showed significant antimicrobial activity against MRSA (12.00 ± 0.00 mm; p-value of < 0.005; Minimum Inhibitory Concentration of 0.391 mg/ml; Minimum Bactericidal Concentration of 1.563 mg/ml). The extract were not cytotoxic at 100 μg/ml which was the highest tested concentration. In acute dermal irritation testing, the methanol extract resulted in mild irritation with erythema and flaking that cleared within 8 days. There were no observable adverse effects from oral administration of the extracts (acute oral toxicity testing) at concentrations of 50 mg/kg, 300 mg/kg and 2000 mg/kg. Tannins, saponins, flavonoids, terpenoids, glycosides, alkaloids and phenols were detected in the methanol extract. Conclusions Antimicrobial activity of R. vulgaris extracts supports its traditional use as a toothbrush. Cytotoxicity demonstrated by the extracts as well as the mild skin irritation warrants further study before R. vulgaris can be recommended for the development of effective and safe mouthwashes.

Sign in / Sign up

Export Citation Format

Share Document