scholarly journals Biochemical Assessment of Renal and Liver Function among Preeclamptics in Lagos Metropolis

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Oloruntoba Ayodele Ekun ◽  
Oluwatumininu Mary Olawumi ◽  
Christian Chigozie Makwe ◽  
Nkeiruka Ogochukwu Ogidi
Keyword(s):  
Uric Acid ◽  
Liver Function ◽  
Total Protein ◽  
Venous Blood ◽  
Urine Samples ◽  
Plasma Sodium ◽  
Cross Sectional ◽  
Blood Samples ◽  
Spot Urine ◽  
Specific Syndrome

Objectives. Preeclampsia is a pregnancy specific syndrome. Studies have shown that preeclampsia has multiorgan dysfunction effects. This study evaluated biomarkers of renal and liver function among preeclamptic Nigerian women. Study Design. This was a cross-sectional study conducted among 49 preeclamptic women and 50 normotensive healthy pregnant women. Method. The baseline data comprising age, gestational age, and blood pressure were obtained. Venous blood and spot urine samples were collected from each participant. Plasma obtained from blood samples taken into lithium heparinized vacutainer bottles was assayed for electrolytes, urea, creatinine, total protein, albumin, and uric acid, while sera samples from blood samples taken into serum separation tube- (SST-) gel vacutainer were assayed for aspartate transaminase and alanine transaminase using ion selective electrode technique and Cobas autoanalyzer. Spot urine samples were assayed for protein and creatinine using Pyrogallol’s reagent and Jaffe’s methods, respectively. Microalbuminuria (protein/creatinine ratio) was generated from spot urine protein and creatinine data. Result. The plasma sodium, total protein, and albumin in preeclamptic group were significantly decreased (p<0.05) when compared with control. There was statistically significant increase (p<0.05) in microalbuminuria, plasma potassium, urea, creatinine, uric acid levels, serum AST, and ALT activities in preeclamptic group. A positive association (p<0.05) between alanine aminotransferase and biomarkers of renal function was observed. Conclusion. Preeclampsia has deleterious effects on renal and liver function as shown by alteration of these parameters.

scholarly journals Comparison of capillary, venous and buffy coat blood samples in detecting Plasmodium species among malaria suspected patients attending at Hamusite health center. A cross-sectional study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Getu Abeje ◽  
Woyneshet Gelaye ◽  
Getaneh Alemu
Keyword(s):  
Health Center ◽  
Detection Rate ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Buffy Coat ◽  
Blood Film ◽  
Capillary Blood ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples

Abstract Background Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodium parasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. Methods A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. Results Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. Conclusion Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.

Changes in electrolyte concentrations and hydration status in endurance horses following transport and an overnight stay prior to competition

2021 ◽  
pp. 1-6
Author(s):  
C. Langdon Fielding ◽  
K. Gary Magdesian
Keyword(s):  
Total Protein ◽  
Hydration Status ◽  
Plasma Sodium ◽  
Bicarbonate Concentration ◽  
Sodium Potassium ◽  
Urea Nitrogen ◽  
Blood Samples ◽  
Time Points ◽  
Nitrogen Concentrations ◽  
Subsequent Elimination

Abstract OBJECTIVE To evaluate changes in electrolyte concentrations and hydration status that take place in endurance horses prior to the start of a competition and determine whether these changes would be associated with elimination. ANIMALS 19 horses entered in the 2016 Tevis Cup 100-Miles (160 km) One-Day Western States Trail Ride. PROCEDURES Heparinized blood samples were collected at 5 time points: prior to transport to the ride (T0), during check-in the day before the ride (T1), 1 to 2 hours before the start of the ride (T2), at the 15-km mark (T3), and at the 55-km mark (T4). Packed cell volume and plasma sodium, potassium, chloride, urea nitrogen, glucose, bicarbonate, and total protein concentrations were determined and compared across time points and between finishers and nonfinishers. RESULTS Signif icant differences were detected among plasma sodium, potassium, and urea nitrogen concentrations measured prior to the start of the ride (ie, T0, T1, and T2). For all variables except chloride and bicarbonate concentrations, significant differences were detected between values obtained prior to the start of the ride and values obtained during the ride (ie, T3 and T4). Only bicarbonate concentration at the 15-km mark of the ride was significantly associated with finishing status. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that significant changes in plasma sodium, potassium, and urea nitrogen concentrations can occur in endurance horses during transport to a competition and when horses are stabled overnight before an event. Additionally, a lower bicarbonate concentration following a steep climb early during the ride was associated with subsequent elimination.

scholarly journals Mean Dietary Salt Intake in Vanuatu: A Population Survey of 755 Participants on Efate Island

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 916
Author(s):  
Katherine Paterson ◽  
Nerida Hinge ◽  
Emalie Sparks ◽  
Kathy Trieu ◽  
Joseph Alvin Santos ◽  
...  
Keyword(s):  
Salt Intake ◽  
Urine Samples ◽  
World Health ◽  
Dietary Salt ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Middle Income ◽  
Dietary Salt Intake ◽  
Spot Urine ◽  
Salt Consumption

Non-communicable diseases are responsible for 63% of global deaths, with a higher burden in low- and middle-income countries. Hypertension is the leading cause of cardiovascular-disease-related deaths worldwide, and approximately 1.7 million deaths are directly attributable to excess salt intake annually. There has been little research conducted on the level of salt consumption amongst the population of Vanuatu. Based on data from other Pacific Island countries and knowledge of changing regional diets, it was predicted that salt intake would exceed the World Health Organization’s (WHO) recommended maximum of 5 g per day. The current study aimed to provide Vanuatu with a preliminary baseline assessment of population salt intake on Efate Island. A cross-sectional survey collected demographic, clinical, and urine data from participants aged 18 to 69 years in rural and urban communities on Efate Island in October 2016 and February 2017. Mean salt intake was determined to be 7.2 (SD 2.3) g/day from spot urine samples, and 5.9 (SD 3.6) g/day from 24-h urine samples, both of which exceed the WHO recommended maximum. Based on the spot urine samples, males had significantly higher salt intake than females (7.8 g compared to 6.5 g; p < 0.001) and almost 85% of the population consumed more than the WHO recommended maximum daily amount. A coordinated government strategy is recommended to reduce salt consumption, including fiscal policies, engagement with the food industry, and education and awareness-raising to promote behavior change.

scholarly journals Effect of Dietary Extruded Linseed and Walnut Meal Mixture (8:1) on Performance and Plasma Protein Profile in Weaned Piglets

2018 ◽  
Vol 75 (2) ◽  
pp. 121 ◽  
Author(s):  
Anca GHEORGHE ◽  
Mihaela HĂBEANU ◽  
Nicoleta Aurelia LEFTER ◽  
Daniela Mihaela GRIGORE
Keyword(s):  
Uric Acid ◽  
Plasma Protein ◽  
Soybean Meal ◽  
Total Protein ◽  
Total Bilirubin ◽  
Protein Profile ◽  
Weaned Piglets ◽  
Urea Nitrogen ◽  
Blood Samples ◽  
Dietary Nitrogen

The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on performance and plasma protein profile in weaned piglets was evaluated for 21 d. Topigs piglets (n=40; BW=8.02±0.82 kg), age 30±3 days, were allotted into 2 groups and fed 2 diets: control [C, based on corn-triticale-soybean meal (SBM)] and experimental (ELS:WM, where the ELS:WM mixture (8:1) partially replace SBM). Blood samples were collected at 7d and 21d after weaning. The plasma protein profile (total protein, total bilirubin, albumin, creatinine, uric acid, urea nitrogen-BUN) were determined by a chemistry analyser. Dietary ELS:WM mixture improve the BW (P=0.047) and ADG (P=0.036) of piglets at 21d after weaning vs C, whereas ADFI and F:G ratio were increase (P>0.05). The plasma protein profile of piglets fed dietary mixture was not affected at 7d and 21d after weaning, except plasma BUN concentration that was decrease (P=0.027) at 21d after weaning. Lower BUN concentration indicated higher availability of dietary nitrogen reflected in a higher deposition of protein. We concluded that dietary ELS:WM mixture improve piglets performance and positively afect plasma protein profile, especially BUN in weaning period.

scholarly journals The Association Between Uric Acid and Symmetric Dimethylarginine Levels in the Patients Undergoing Twice-weekly Hemodialysis

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Aida Lydia ◽  
Yassir Yassir ◽  
Rudy Hidayat ◽  
Suhendro Suwarto
Keyword(s):  
Uric Acid ◽  
Venous Blood ◽  
Tertiary Hospital ◽  
Cross Sectional Study ◽  
Hemodialysis Patients ◽  
Bivariate Analysis ◽  
Symmetric Dimethylarginine ◽  
Cross Sectional ◽  
Increased Risk ◽  
History Of

Background: Uric acid (UA) levels are associated with increased risk of cardiovascular events and mortality in hemodialysis patients. However, there are still conflicting data on the mechanism of increased risks related to uric acid levels. Objectives: This study assessed the association between uric acid levels and symmetric dimethylarginine (SDMA), as a marker of cardiovascular disease, in the subjects undergoing hemodialysis twice weekly. Methods: This was a cross-sectional study conducted in a tertiary hospital in Jakarta, Indonesia. We included all the adults who underwent hemodialysis twice weekly for at least three months in our hospital. Subjects already on uric acid lowering therapy, pregnant or lactating women and those with a history of malignancy were excluded. Uric acid and SDMA levels were measured at the same time in pre-dialysis venous blood samples. Bivariate analysis was performed using the Mann-Whitney U test or one-way ANOVA. Results: A total of 126 subjects were included. The median level of UA was 8.4 mg/dL (IQR: 2.6, min: 4.1, max: 13.6), and 72 subjects (57.14%) had UA levels of 8 mg/dL or higher. The median SDMA level was 535.5 (312.7) mmol/dL (min: 119.7, max: 1895.5). Subjects with UA levels > 8 mg/dL had significantly higher SDMA levels compared to subjects with UA levels < 8 mg/dL (550.1 (IQR: 357.25) vs 491.35 (IQR: 181.1), P: 0.0475). Conclusions: In twice-weekly hemodialysis patients, UA levels above 8 mg/dL were associated with increased SDMA levels.

Metabolic response to maximal exercise of 800 and 2,000 m in the thoroughbred horse

1987 ◽  
Vol 63 (1) ◽  
pp. 12-19 ◽  
Author(s):  
R. C. Harris ◽  
D. J. Marlin ◽  
D. H. Snow
Keyword(s):  
Uric Acid ◽  
Maximal Exercise ◽  
Metabolic Response ◽  
Venous Blood ◽  
Glycerol Content ◽  
Muscle Lactate ◽  
Blood Samples ◽  
Thoroughbred Horse ◽  
Exercise Muscle ◽  
Muscle Biopsies

To define the metabolic response to maximal exercise in the thoroughbred horse under field conditions, muscle biopsies and venous blood samples were taken from five horses after a single 800-m gallop and from four horses after a single 2,000-m gallop. Muscle and blood samples were also collected during 60 min of recovery. After exercise muscle ATP contents were decreased by 30 +/- 7 (SD) and 47 +/- 3% after the 800- and 2,000-m gallops, respectively. As indicators of purine catabolism, ammonia and uric acid increased in plasma, the accumulation being greater after the 2,000-m gallop. Blood ammonia peaked immediately after exercise and uric acid after 40–60 min of recovery. Muscle glycogen utilization over the 800- and 2,000-m gallops averaged 2.68 +/- 0.90 and 1.06 +/- 0.12 mmol glucosyl units.kg dry muscle-1.s-1, respectively, and the total used amounted to 27.3 +/- 6.6 and 32.5 +/- 8.8% of the initial store. Muscle lactate accumulation averaged 123.5 +/- 49.7 and 167.3 +/- 20.7 mmol/kg dry muscle, respectively, and declined during recovery with half times of 22.9 +/- 4.2 and 18.9 +/- 6.6 min. Blood lactate peaked 5–10 min after exercise. Exercise resulted in only a small increase in muscle glycerol content, but this continued to rise during recovery reaching 9–12 mmol/kg dry muscle after 20 min. During this time the increase in muscle glycerol content exactly matched the decline in glycerol 3-phosphate.

scholarly journals Reliability of studies of iodine intake and recommendations for number of samples in groups and in individuals

2007 ◽  
Vol 99 (4) ◽  
pp. 813-818 ◽  
Author(s):  
Stig Andersen ◽  
Jesper Karmisholt ◽  
Klaus M. Pedersen ◽  
Peter Laurberg
Keyword(s):  
Standard Deviation ◽  
Urinary Iodine ◽  
Iodine Intake ◽  
Urine Samples ◽  
Urinary Iodine Excretion ◽  
Iodine Nutrition ◽  
Cross Sectional ◽  
Spot Urine ◽  
Iodine Excretion ◽  
And Gender

The iodine intake level in a population is determined in cross-sectional studies. Urinary iodine varies considerably and the reliability of studies of iodine nutrition and the number of samples needed is unsettled. We performed a longitudinal study of sixteen healthy men living in an area of mild to moderate iodine deficiency. Iodine and creatinine concentrations were measured in spot urine samples collected monthly for 13 months. From these data we calculated the number of urine samples needed to determine the iodine excretion level for crude urinary iodine and for 24 h iodine excretion estimated from age- and gender-specific creatinine excretions. We found that mean urinary iodine excretion varied from 30 to 87 μg/l (31 to 91 μg/24 h). Sample iodine varied from 10 to 260 μg/l (20 to 161 μg/24 h). Crude urinary iodine varied more than estimated 24 h iodine excretion (population standard deviation 32v. 26; individual standard deviation 29v. 21; Bartlett's test,P < 0·01 for both). The number of spot urine samples needed to estimate the iodine level in a population with 95 % confidence within a precision range of ± 10 % was about 125 (100 when using estimated 24 h iodine excretions), and within a precision range of ± 5 % was about 500 (400). A precision range of ± 20 % in an individual required twelve urine samples or more (seven when using estimated 24 h iodine excretions). In conclusion, estimating population iodine excretion requires 100–500 spot urine samples for each group or subgroup. Less than ten urine samples in an individual may be misleading.

scholarly journals The Effect of Submaximal Exercise on Blood Creatinine, Urea, Total Protein and Uric Acid Levels of Trained and Untrained Subjects

2018 ◽  
Vol 3 (66) ◽  
Author(s):  
Alina Gailiūnienė ◽  
Arvydas Stasiulis ◽  
Jolanta Michailovienė
Keyword(s):  
Renal Function ◽  
Uric Acid ◽  
Total Protein ◽  
Nitrogen Compounds ◽  
Blood Samples ◽  
Before And After ◽  
Group 2 ◽  
Exercise Induced ◽  
Blood Creatinine ◽  
Group 1

There are numerous studies about exercise-induced sports hematuria, proteinuria, acute renal failure following a marathon (Steward, Posen, 1980; Poortmans et al., 2001; Ayca et al., 2006). But studies investigating the effects of exercise on blood indicators of renal function are quite few.The aim of this study was to investigate the effects of submaximal veloergometric exercise on very important bio-chemical indicators of renal function — level nitrogen compounds in the blood. We investigated concentration of creatinine, urea, total protein and uric acid in venous blood samples before and after submaximal veloergometric exercise. Those nitrogen compounds were studied in three groups of subjects.The study was performed with 10 trained (Group 1), 10 untrained subjects (Group 2) and 10 subjects with I o  hiper-tensive status (Group 3). The age range was 20.5—21.3 years, weight — 71.8—77.3 kg, height — 180—177 cm. All subjects voluntered to participate in the study after providing written informed consent. The study was approved in accordance with the Declaration of Helsinki. Blood samples were collected before and after the submaximal velo-ergometric test into vacumtrainer tubes. Concentrations of creatinine, urea, total protein and uric acid in the serum were determined using Technicon Auto Analyzer ADVIA 1650 system.All data were reported as mean ± standard deviation (SD) unless otherwise specifi ed, and statistical signifi cance was recognized when p ≤ 0.05.No statistically signifi cant difference was observed between pre- and post exercise blood creatinine, urea, total protein and uric acid mean levels of all group subjects. A marked exercise induced increase in blood creatinine and total protein concentrations was observed when the results of trained and untrained participants’ parameter differences were compared after the exercise.A signifi cant (p < 0.05) exercise-induced increase in blood urea and total protein concentration was observed when the mean values of Group 1 and Group 2 before the exercise and parameters after the exercise were compared.When blood creatinine, urea, total protein and uric acid levels were compared separately for the participants, it was observed that seven persons in Group 1 and three persons in Group 2 showed a marked exercise-induced increase in the blood nitrogen compounds level.Research results suggest that 1) the testing exercise-induced statistically insignifi cant (p > 0.05) increases in the blood parameters of nitrogen compounds (creatinine, urea, total protein and uric acid) could be due to the common phenomenon of the physical stress and catecholamine effects, 2) postexercise changes of blood nitrogen compounds were signifi cant (p < 0.05) when the results of Group 1 with Group 2 participants were compared. The signifi cant differences in metabolic responce in Group 1 and  Group 2 participants probably refl ect differences in work volume and intensity, and 3) further studies are needed to be performed on more subjects to evaluate exercise-specifi c effects on postexercise changes of blood nitrogen compounds in athletes and nonathletes.Keywords: blood, creatinine, urea, total protein, uric acid. 

scholarly journals Evaluation of Serum Interleukin-6 and C-Reactive Protein Levels among Women During Term Labour

2020 ◽  
pp. 69-76
Author(s):  
Ubongabasi A. James ◽  
John O. Imaralu ◽  
Ijeoma Esiaba
Keyword(s):  
Interleukin 6 ◽  
Venous Blood ◽  
University Teaching ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Reactive Protein ◽  
Cross Sectional ◽  
Blood Samples ◽  
Reactive Protein ◽  
Significant Difference ◽  
Term Labour

Aims: To determine the serum concentrations of C-reactive protein (CRP) and interleukin 6 (IL-6) as well as level of leukocytosis, as inflammatory contributory factors in women with uncomplicated term pregnancies before, during and after labour. Study Design:  This is a cross-sectional study. Place and Duration of Study: The study was carried out in the Department of Obstetrics and Gynaecology of Babcock University Teaching Hospital (BUTH) and Department of Biochemistry, Babcock University, Ogun State Nigeria between June 2019 and February 2020. Methodology: 45 venous blood samples were obtained from 34 selected women and grouped into three; prenatal (≥ 32 weeks, n = 18), labour (4 to 6 cm dilation, n = 12) and postnatal (≤ 24 hour postpartum, n = 15). Sixteen blood samples were also obtained from the umbilical cord. Levels of CRP and IL-6 were determined by Enzyme Linked Immunosorbent Assay (ELISA) techniques and the leukocyte count, by hematologic method. Differences in statistical mean were evaluated by one way analysis of variance (ANOVA) with Bonferroni post hoc comparison. The level of significance was set at P < 0.05. Results: Concentration of serum IL-6 was significantly high during labour (1354.79 ± 189.16 pg/mL) compared to the prenatal (14.94 ± 4.86 pg/mL, P < .001) and postnatal (13.17 ± 3.06 pg/mL, P < .001) periods. The low level of CRP observed during active labour compared to the prenatal and postnatal periods did not reach significant difference. The levels of these inflammatory markers were low in the cord blood. Leukocyte counts (P = .011) as well as neutrophils (P = .014) and MID cell fractions (P = .004) were significantly higher during the postnatal period. Conclusion: The high levels of serum IL-6 observed in this study supports human term labour as an inflammatory event not associated with increased leukocytosis.

scholarly journals Hyperuricemia in Systemic Hypertension and its correlation with systolic and diastolic blood pressure.

2020 ◽  
Vol 27 (01) ◽  
pp. 89-93
Author(s):  
Haji Khan Khoharo ◽  
Ali Akbar Shah ◽  
Fatima Qureshi ◽  
Sajjad Ali Almani
Keyword(s):  
Blood Pressure ◽  
Uric Acid ◽  
Diastolic Blood Pressure ◽  
Serum Uric Acid ◽  
Study Design ◽  
Venous Blood ◽  
University Hospital ◽  
Systemic Hypertension ◽  
Cross Sectional ◽  
Positive Correlation

Objectives: To determine the serum uric acid (SUA) in systemic hypertension and its correlation with systolic (SBP) and diastolic blood pressure (DBP). Study Design: Cross sectional study design. Setting: Department of Medicine, Isra University Hospital. Period: From April 2016 – February 2017. Material and Methods: A sample of 100 cases of systemic hypertension and 100 age, gender, body weight and BMI controls were selected through non-probability purposive sampling. Volunteers were asked for history, physical examination, and blood sampling. Systemic BP was measured with a mercury sphygmomanometer. 2 ml venous blood was taken, centrifuged and sera were used for detection of SUA. Data was saved in a pre- structured Performa. Computed based statistical software (SPSS v 22.0, IBM, Incorporation, USA) was used for data analysis. Data variables were analyzed at 95% CI (P ≤ 0.05). Results: Serum uric acid in controls was 2.91±0.75 mg/dl compared to 5.70±1.76 mg/dl (P=0.0001). 57% of cases revealed hyperuricemia compared to 11% in control (X2=17.5, P=0.0001). Serum Uric acid showed significantly positive correlation with Systolic BP (r= 0.518*, p=0.0001) and Diastolic BP (r= 0.397**, p=0.0001). Conclusion: The present study reports hyperuricemia in 57% cases of systemic hypertension and uric acid shows positive correlation with systolic and diastolic blood pressure.

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