scholarly journals Effects of Juniperus phoenicea Hydroalcoholic Extract on Inflammatory Mediators and Oxidative Stress Markers in Carrageenan-Induced Paw Oedema in Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Karama Zouari Bouassida ◽  
Samar Makni ◽  
Amina Tounsi ◽  
Lobna Jlaiel ◽  
Mohamed Trigui ◽  
...  
Keyword(s):  
Antioxidant Enzyme Activities ◽  
Phytochemical Analysis ◽  
Protective Effects ◽  
Oedema Formation ◽  
Stress Markers ◽  
Paw Oedema ◽  
Juniperus Phoenicea ◽  
Wild Tree ◽  
Mda Content

Juniperus phoenicea (J. phoenicea) is a wild tree belonging to the Cupressaceae family, commonly used for the treatment of several disorders. This study aimed to evaluate the potential protective effects of J. phoenicea hydroethanolic extract (EtOH-H2OE) against oxidation, acute inflammation, and pain in mice models. For the purpose, chemical compounds of J. phoenicea EtOH-H2OE were also analyzed by GC-MS. The J. phoenicea EtOH-H2OE showed a potent antioxidant activity in vitro, thanks to its richness in phenolic and flavonoid compounds. Mice treated with EtOH-H2OE (100 mg/kg BW) showed reduced paw oedema formation and decreased malondialdehyde (MDA) content. The evaluation of antioxidant enzyme activities in paw oedema tissue after five hours of carrageenan induction showed a significant increase (P<0.05). Inflammatory biomarkers explorations of J. phoenicea EtOH-H2OE-treated mice showed a restoration of the studied parameters to near-normal values. Furthermore, EtOH-H2OE of J. phoenicea produced a significant reduction of the number of abdominal writhes (P<0.05) in a dose-dependent way. Phytochemical analysis of the J. phoenicea EtOH-H2OE by GC-MS showed the presence of hexadecanoic and stearic acids known as anti-inflammatory and analgesic compounds. Our investigation provided evidence that J. phoenicea EtOH-H2OE can effectively reduce the inflammation and pain in mice models.

scholarly journals Anti-Inflammatory Activity of Pleurotus ostreatus, a Culinary Medicinal Mushroom, in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
W. J. A. Banukie N. Jayasuriya ◽  
Shiroma M. Handunnetti ◽  
Chandanie A. Wanigatunge ◽  
Gita H. Fernando ◽  
D. Thusitha U. Abeytunga ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Inflammatory Activity ◽  
No Production ◽  
Protective Effects ◽  
Paw Oedema ◽  
Freeze Dried ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Context. Pleurotus ostreatus (P.o) is a culinary mushroom which is commonly called as “oyster mushroom” belonging to the Basidiomycetous fungi of the order Agaricales and family Pleurotaceae. Objectives. The present study investigates the anti-inflammatory potential of P.o and the underlying mechanisms of activity. Materials and Methods. Anti-inflammatory activity was evaluated using suspensions of freeze-dried and powdered (SFDP) P.o and acetone extract (AE) of P.o in normal and alloxan-induced diabetic rats using the carrageenan-induced rat paw oedema model. The mechanisms by which P.o is mediating the anti-inflammatory activity were studied using in vivo and in vitro assays. Results. At doses of 500–1000 mg/kg, the SFDP of P.o showed long-lasting activity at both early and late phases of carrageenan-induced rat paw oedema. The dose of 750 mg/kg showed the most potent inhibitory activity (92% inhibition) in healthy rats. The AE of P.o showed maximum inhibition of oedema of 87%. P.o exerted protective effects on the inflammatory pathologies in rats with diabetes. The possible mechanisms by which P.o mediates the anti-inflammatory activity were antihistamine activity (52.1%), inhibition of cell migration to the site of inflammation (45.4%), in vitro membrane stabilizing activity (52.6%), and inhibition of nitric oxide (NO) production (91.2%) (P<0.05). Dose-dependent inhibition of NO production was seen with in vitro treatment of rat peritoneal cells with AE of P.o (r = 0.95; P<0.05). Discussion and Conclusion. The promising activity of culinary mushroom P.o against inflammation suggests its potential application as a functional food during inflammatory conditions.

scholarly journals In Vitro Effects of Brassinosteroids on the Growth and Antioxidant Enzyme Activities in Groundnut

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Aman Verma ◽  
C. P. Malik ◽  
V. K. Gupta
Keyword(s):  
Antioxidant Enzyme ◽  
Chlorophyll Content ◽  
Enzyme Activities ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Hill Reaction ◽  
Antioxidant Enzyme Activities ◽  
Mda Content ◽  
In Vitro Effects

This paper deals with the in vitro effects of brassinosteroids (BRs) on growth in the form of multiple shoots, chlorophyll content, Hill reaction activity (HRA), activities of catalase (CAT), peroxidase (POX), polyphenol oxidase (PPX), and ascorbate peroxidase (APX) in Arachis hypogaea L. genotypes (M-13 and PBS24030). In vitro impact of BR on shoot multiplication potential was found to be the best at 1 mL L−1 with BA (3 mg L−1) in both the cultivars. Flowering was observed in PBS24030 on the medium containing 2.0 mL L−1 BR with 3 mg L−1 BA. Rhizogenesis was noticed in the presence of BR alone. Total chlorophyll content and HRA were highest at 2.0 mL L−1 with BA in M-13 and 1.0 mL L−1 with BA in PBS24030. Antioxidant enzyme activities were increased in the presence of BR whether alone or in combination with BA in both the cultivars. However, progressive decline was observed in case of MDA content. The results obtained in the study clearly indicated not only the in vitro establishment of groundnut cultivars in the presence of BR alone and in combination with BA but also its effect on various growth promotory physiological parameters.

scholarly journals Allicin Protects PC12 Cells Against 6-OHDA-Induced Oxidative Stress and Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics

2015 ◽  
Vol 36 (3) ◽  
pp. 966-979 ◽  
Author(s):  
Hao Liu ◽  
Ping Mao ◽  
Jia Wang ◽  
Tuo Wang ◽  
Chang-Hou Xie
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
Pc12 Cells ◽  
Atp Synthesis ◽  
Ros Generation ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Cytochrome C Release ◽  
Endogenous Antioxidant

Background: Parkinson disease (PD) is a common adult-onset neurodegenerative disorder, and PD related neuronal injury is associated with oxidative stress and mitochondrial dysfunction. Allicin, the main biologically active compound derived from garlic, has been shown to exert various anti-oxidative and anti-apoptotic activities in in vitro and in vivo studies. Methods: The present study aimed to investigate the potential protective role of allicin in an in vitro PD model induced by 6-hydroxydopamine (6-OHDA) in PC12 cells. The protective effects were measured by cell viability, decreased lactate dehydrogenase (LDH) release and flow cytometry, and the anti-oxidative activity was determined by reactive oxygen species (ROS) generation, lipid peroxidation and the endogenous antioxidant enzyme activities. Mitochondrial function in PC12 cells was detected by mitochondrial membrane potential (MMP) collapse, cytochrome c release, mitochondrial ATP synthesis, and the mitochondrial Ca2+ buffering capacity. To investigate the potential mechanism, we also measured the expression of mitochondrial biogenesis factors, mitochondrial morphological dynamic changes, as well as detected mitochondrial dynamic proteins by western blot. Results: We found that allicin treatment significant increased cell viability, and decreased LDH release and apoptotic cell death after 6-OHDA exposure. Allicin also inhibited ROS generation, reduced lipid peroxidation and preserved the endogenous antioxidant enzyme activities. These protective effects were associated with suppressed mitochondrial dysfunction, as evidenced by decreased MMP collapse and cytochrome c release, preserved mitochondrial ATP synthesis, and the promotion of mitochondrial Ca2+ buffering capacity. In addition, allicin significantly enhanced mitochondrial biogenesis and prevented fragmentation of mitochondrial network after 6-OHDA treatment. The results of western blot analysis showed that the 6-OHDA induced decrease in the expression of optic atrophy type 1 (Opa-1), increase in mitochondrial fission 1 (Fis-1) and dynamin-related protein 1 (Drp-1) were all partially revised by allicin. Conclusion: In summary, our data strongly suggested that allicin treatment can exert protective effects against PD related neuronal injury through inhibiting oxidative stress and mitochondrial dysfunction with dynamic changes.

scholarly journals Polysaccharides with Antioxidative and Antiaging Activities from Enzymatic-Extractable Mycelium by Agrocybe aegerita (Brig.) Sing

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Huijuan Jing ◽  
Qing Zhang ◽  
Min Liu ◽  
Jianjun Zhang ◽  
Chen Zhang ◽  
...  
Keyword(s):  
Functional Foods ◽  
Antioxidant Activities ◽  
Monosaccharide Composition ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Organ Protection ◽  
Agrocybe Aegerita ◽  
Dose Dependent ◽  
Water Extractable

This study aimed to investigate the antioxidant, antiaging, and organ protective effects of the water-extractable mycelium polysaccharides (MPS) and enzymic-extractable mycelium polysaccharides (En-MPS) by Agrocybe aegerita (Brig.) Sing in D-galactose-induced (D-gal-induced) aging mice. In in vitro assays, the En-MPS demonstrated stronger antioxidant activities in dose-dependent manners. The mice experiments revealed that both En-MPS and MPS had potential effects on antioxidation, antiaging, and organ protection mainly by improving the antioxidant enzyme activities, decreasing the lipid peroxidation, and remitting the lipid metabolism. Furthermore, chemical composition and monosaccharide composition of polysaccharides were also measured, and the results indicated that differences in biological activity of MPS and En-MPS samples showed a significant correlation to their purity. The findings demonstrated that the polysaccharides by A. aegerita (Brig.) Sing could be exploited as natural and functional foods for the prevention and alleviation of aging and its complications.

scholarly journals Characterization, Antioxidant, Anti-Aging and Organ Protective Effects of Sulfated Polysaccharides from Flammulina velutipes

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3517 ◽  
Author(s):  
Fangfang Yuan ◽  
Zheng Gao ◽  
Wenbo Liu ◽  
Huaping Li ◽  
Yiwen Zhang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Average Molecular Weight ◽  
Reducing Power ◽  
Flammulina Velutipes ◽  
Sulfated Polysaccharides ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Characteristic Functional

As an irreversible and complex degenerative physiological process, the treatment for aging seems strategically necessary, and polysaccharides play important roles against aging owing to their abundant bioactivities. In this paper, the antioxidant and anti-aging activities of Flammulina velutipes polysaccharides (FPS) and its sulfated FPS (SFPS) on d-galactose-induced aging mice were investigated. The in vitro antioxidant activities demonstrated that SFPS had strong reducing power and superior scavenging effects on 2, 2-diphenylpicrylhydrazyl (DPPH), hydroxyl radicals and the chelating activities of Fe2+. The in vivo animal experiments manifested that the SFPS showed superior antioxidant and protective abilities against the d-galactose-induced aging by increasing the antioxidant enzyme activities, decreasing lipid peroxidation, improving the inflammatory response and ameliorating the anile condition of mice. Furthermore, the structural analysis of SFPS was investigated through FT-IR, NMR, and HPLC analysis, and the results indicated that SFPS was a homogeneous heteropolysaccharide with a weight-average molecular weight of 2.81 × 103 Da. Furthermore, SFPS has also changed in characteristic functional groups and monosaccharide composition compared to FPS. These results suggested that sulfated modification could enhance the anti-oxidation, anti-aging and protective activities of F. velutipes polysaccharides, which may provide references for the development of functional foods and natural medicines.

scholarly journals Protective Effects of Fruit Wines against Hydrogen Peroxide—Induced Oxidative Stress in Rat Synaptosomes

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1414
Author(s):  
Uroš Čakar ◽  
Mirjana Čolović ◽  
Danijela Milenković ◽  
Branislava Medić ◽  
Danijela Krstić ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Glutathione Peroxidase ◽  
Membrane Damage ◽  
Principal Component ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Sod Activity ◽  
Black Chokeberry

This study aimed to evaluate, in vitro, the antioxidative potential of fruit wines produced from berry fruits (i.e., black chokeberry, blueberry, blackberry, and raspberry), cherry, and apple by different technological processes. For this purpose, the activities of antioxidant enzymes (catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD)) and malondialdehyde (MDA) content as a marker of membrane damage were determined in wine-treated synaptosomes with hydrogen peroxide-induced oxidative stress. All studied wines induced increased antioxidant enzyme activities and decreased MDA levels compared to hydrogen peroxide-treated synaptosomes (i.e., control). The highest SOD activity was observed in synaptosomes treated with blackberry wine (6.81 U/mg), whereas blueberry wine induced the highest catalase and glutathione peroxidase activities (0.058 U/mg and 0.017 U/mg, respectively). Black chokeberry proved to be the best in lipid peroxidation protection with the lowest MDA value (1.42 nmol/mg). Finally, principal component analysis and hierarchical cluster analysis additionally highlighted a higher antioxidant capacity of wines produced from dark-skinned fruits (i.e., blackberry, black chokeberry, and blueberry). The results suggest protective effects of the fruit wines against oxidative damage, and, accordingly, their promising application as functional food.

Protective effects of casein-derived peptide VLPVPQK against hydrogen peroxide–induced dysfunction and cellular oxidative damage in rat osteoblastic cells

2017 ◽  
Vol 36 (9) ◽  
pp. 967-980 ◽  
Author(s):  
SB Mada ◽  
S Reddi ◽  
N Kumar ◽  
S Kapila ◽  
R Kapila
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Osteoblastic Cells ◽  
Antioxidant Enzyme Activities ◽  
Protective Agent ◽  
Protective Effects ◽  
Differentiation Markers

Oxidative stress inhibits osteoblast differentiation and function that lead to the development of osteoporosis. Casein-derived peptide VLPVPQK (PEP), a potent antioxidant, was isolated from β-casein of buffalo milk. We used an in vitro oxidative stress model induced by hydrogen peroxide (H2O2) in rat osteoblastic cells to investigate the protective effects of PEP against H2O2-induced dysfunction and oxidative damage. Cells were pretreated with PEP (50–200 ng/mL) for 2, 7 or 21 days followed by 0.3 mM H2O2 treatment for 24 h and then markers of osteogenic development, oxidative damage and apoptosis were examined. PEP significantly increased the viability and differentiation markers of osteoblast cells such as alkaline phosphatase and calcium mineralization. Moreover, PEP suppressed the production of reactive oxygen species (ROS), lipid peroxidation and ameliorated H2O2-induced reduction in glutathione, superoxide dismutase and catalase activities. In addition, PEP partially inhibited caspase-9 and-3 activities and reduced propidium iodide–positive cells. Altogether, our results demonstrated that PEP could protect rat osteoblast against H2O2-induced dysfunction and oxidative damage by reduction of ROS production, lipid peroxidation and increased antioxidant enzyme activities. Thus, our data suggest that PEP might be a valuable protective agent against oxidative stress–related diseases such as osteoporosis.

scholarly journals Effect of Superoxide Dismutase on Semen Parameters and Antioxidant Enzyme Activities of Liquid Stored (5°C) Mithun (Bos frontalis) Semen

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
P. Perumal
Keyword(s):  
Superoxide Dismutase ◽  
Membrane Integrity ◽  
Semen Parameters ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Total Antioxidant ◽  
Biochemical Profiles ◽  
Group 2

The present study was undertaken to assess the effect of superoxide dismutase (SOD) on sperm motility, and viability; total sperm abnormality; acrosomal and plasma membrane integrity; DNA abnormality; antioxidant profiles such as catalase (CAT), reduced glutathione (GSH), and total antioxidant capacity (TAC); enzymatic profiles such as aspartate amino transaminase (AST), and alanine amino transaminase (ALT); and biochemical profiles such as malondialdehyde (MDA) production and cholesterol efflux. Total numbers of 50 ejaculates were collected twice a week from eight mithun bulls and semen was split into four equal aliquots, diluted with the TEYC extender. Group 1: semen without additives (control), and group 2 to group 4: semen was diluted with 50 U/mL, 100 U/mL, and 150 U/mL of SOD, respectively. These seminal parameters, antioxidant, enzymatic, and biochemical profiles were assessed at 5°C for 1, 6, 12, 24, and 30 h of incubation. Inclusion of SOD into diluent resulted in significant (P<0.05) decrease in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities at different hours of storage periods as compared with control group. Additionally, SOD at 100 U/mL has significant improvement in quality of mithun semen than SOD at 50 or 150 U/mL stored in in-vitro for up to 30 h. It was concluded that the possible protective effects of SOD on sperm parameters are that it prevents MDA production and preserves the antioxidants and intracellular enzymes during preservation.

scholarly journals In vitro treatment of 3T3-L1 adipocytes with recombinant Calcium/calmodulin-dependent Protein Kinase IV (CaMKIV) limits ER stress and improves insulin sensitivity through inhibition of autophagy via the mTOR/CREB signaling pathway

2020 ◽  
Author(s):  
Jiali Liu ◽  
Ruihua Yang ◽  
Hao Meng ◽  
Ting Zhou ◽  
Qian He
Keyword(s):  
Insulin Resistance ◽  
Insulin Sensitivity ◽  
Er Stress ◽  
Insulin Signaling ◽  
Protective Effects ◽  
Calcium Calmodulin Dependent ◽  
Stress Markers ◽  
Dependent Protein ◽  
Treatment Of Obesity

Abstract Background: CaMKIV has been identified as a potential regulator of skeletal muscle glucose metabolism and insulin gene expression in the pancreas. However, the mechanism of CaMKIV involved in adipose insulin resistance is not fully understood. Autophagy has to be shown as a potential therapeutic target for ER (endoplasmic reticulum) stress and insulin resistance. The purpose of this study is to investigate the effects of CaMKIV on the ER stress, autophagic function and insulin signaling in tunicamycin treated adipocytes. Methods: In this study, we treated mature 3T3-L1 adipocytes with tunicamycin to induce ER stress. Then recombinant CaMKIV and/or targeted-siRNA of CREB and mTOR were transfected into tunicamycin treated 3T3-L1 adipocytes. The ER stress markers, autophagy activation, mTOR/CREB signaling and insulin sensitivity were analyzed by western blotting or electron microscopy. Results: CaMKIV not only reversed tunicamycin-induced expression of p-PERK, cleaved-ATF6, Atg7 and LC3II, as well as the reduction of p62 expression, but also improved expression of p-Akt and p-IRS-1. Moreover, CaMKIV inhibited activated ER stress and elevated insulin sensitivity inAtg7 siRNA transfected adipocytes. However, the protective effects of CaMKIV were nullified by suppression of mTOR or CREB in tunicamycin-induced adipocytes. Conclusion: This study proves recombinant CaMKIV inhibits ER stress and improves insulin signaling by regulation of autophagy. The protective effect of CaMKIV in adipocytes at least partly through mTOR/CREB signaling, which could be regarded as novel opportunities for treatment of obesity and type 2 diabetes.

Sign in / Sign up

Export Citation Format

Share Document