Supplementation of Stingless Bee Honey from Heterotrigona itama Improves Antiobesity Parameters in High-Fat Diet Induced Obese Rat Model

Heterotrigona itama is a common stingless bee species found in Southeast Asia. Studies on the health benefits of its honey are limited in comparison with other stingless bee species. This study examines the antiobesity benefits found in stingless bee honey (SBH) from H. itama. The parameters used to measure the benefits were weight change, morphological structures, and biochemical characteristics. The research was conducted by using rats that were given a high-fat diet (HFD). In total 48 male Sprague Dawley (SD) rats were given a formulated HFD to increase the levels of obesity, the HFD was administered with a value of 0.68 g/cm2. The duration of the treatment was six weeks, and the results show that the induction obesity using the HFD was successful. Following this, the rats were then treated with SBH (at dosages of 1000 mg/kg, 750 mg/kg or 500 mg/kg), with orlistat or with a placebo. Compared with typical obesity treatment methods, the one that used the three dosages of SBH showed a higher reduction in body mass index (BMI), percentage of body weight gain, adiposity index, and relative organ weight (ROW). The levels of liver enzymes (ALT, AST, and ALP) were also significantly lower in SBH-treated groups. The levels of triglycerides and LDL-cholesterol were significantly lower, while the level of HDL-cholesterol was significantly higher in comparison with the control obese group. In terms of morphological structures, the number of adipocyte cells was reduced, and the hepatocytes found in the liver were less prone to rupturing when treated with SBH. In conclusion, the administration of SBH led to an improvement in indicators associated with obesity reduction. SBH also possesses a hepatoprotective potential which can reduce the health risks related to obesity.

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Lactobacillus Rhamnosus Yoba Modulates Serum Cholesterol in Normo-Lipidemic Wistar Rats Fed a High Fat Diet (P20-013-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz040.p20-013-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Adaku Iwueke ◽

Conrad Miruka ◽

John Ejekwumadu ◽

Ronald Kiiza ◽

Pius Theophilus

Keyword(s):

Body Weight ◽

High Fat Diet ◽

Wistar Rats ◽

Ldl Cholesterol ◽

Lactobacillus Rhamnosus ◽

Hdl Cholesterol ◽

Male Rats ◽

Fermented Foods ◽

Distilled Water ◽

High Fat

Abstract Objectives The study was aimed at assessing the effects of Lactobacillus rhamnosus yoba on the serum lipid profile and body weight of male Wistar rats fed a high fat diet. Methods 20 seven week old male rats weighing between 120 g and 180 g were used for the study and divided into 4 groups of 5 rats each. The control group was fed normal mice pellets and distilled water, while the other groups were fed mice pellets supplemented with 3% cholesterol and 2% saturated fat in addition to any of distilled water, Lactobacillus rhamnosus yoba or Norvastatin respectively. The body weight was measured at the start of the study and after 2 weeks while serum parameters were measured after 8 weeks. Data was analyzed using SPSS Version 20. ANOVA and Tukey's tests determined significant differences in means at 95% confidence interval. Results Lactobacillus rhamnosus yoba significantly (P < 0.005) modulated weight gain, serum total cholesterol and triglycerides when compared to the control. Similarly, LDL-cholesterol was significantly modulated (P < 0.005) while HDL-cholesterol was significantly enhanced (P < 0.005) when compared to the control. Conclusions The ability of Lactobacillus rhamnosus yoba to elevate HDL cholesterol and modulate LDL-cholesterol without the side effects of statins makes it a potential functional food. In line with the findings, the present study justifies the use of Lactobacillus rhamnosus yoba as a probiotic in fermented foods. Funding Sources NA.

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Obesity is the major contributor to vascular dysfunction and inflammation in high-fat diet hypertensive rats

Clinical Science ◽

10.1042/cs20090395 ◽

2009 ◽

Vol 118 (4) ◽

pp. 291-301 ◽

Cited By ~ 57

Author(s):

Ahmed A. Elmarakby ◽

John D. Imig

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

Body Weight Gain ◽

Vascular Dysfunction ◽

Normal Diet ◽

Sprague Dawley ◽

High Fat ◽

Hypertensive Rats ◽

Induced Hypertension ◽

Cox 2

Obesity and hypertension are the two major risk factors that contribute to the progression of end-stage renal disease. To examine whether hypertension further exacerbates oxidative stress and vascular dysfunction and inflammation in obese rats, four groups of male Sprague–Dawley rats were fed either a normal (7% fat) or high-fat (36% fat) diet for 6 weeks and osmotic pumps were implanted to deliver ANG (angiotensin II) or vehicle for an additional 4 weeks. Treatment with the high-fat diet did not alter ANG-induced hypertension compared with the normal diet (174±6 compared with 170±5 mmHg respectively). Treatment with the high-fat diet increased body weight gain and plasma leptin levels and induced insulin resistance in normotensive and ANG-induced hypertensive rats. Plasma TBARS (thiobarbituric acid-reacting substances), a measure of oxidative stress, were elevated in high-fat diet-fed rats compared with controls (11.2±1 compared with 8.4±1 nmol/ml respectively) and was increased further in ANG-induced hypertensive rats fed a high-fat diet (18.8±2.2 nmol/ml). Urinary nitrite excretion was also decreased in rats fed a high-fat diet without or with ANG infusion compared with controls. Afferent arteriolar relaxation to acetylcholine was impaired in rats fed the high-fat diet without or with ANG infusion. Renal cortical TNF-α (tumour necrosis factor-α), COX-2 (cyclo-oxygenase-2) and phospho-IKK (inhibitor of nuclear factor κB kinase) expression increased in high-fat diet-fed rats compared with normal diet-fed rats. The increases in phospho-IKK and COX-2 expression were elevated further in ANG-induced hypertensive rats fed the high-fat diet. These results suggest that ANG-induced hypertension exacerbates oxidative stress and renal inflammation without further impairment in vascular dysfunction in high-fat diet-induced obesity.

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The Antihyperlipidaemic Property of Methanol Leaf Extract of Chrysophyllum albidum in Albino Rats (Wistar Strain) Fed on High Fat Diet.

JOURNAL OF ADVANCES IN BIOLOGY ◽

10.24297/jab.v11i1.6793 ◽

2018 ◽

Vol 11 (1) ◽

pp. 2146-2154

Author(s):

Osebhahiemen Happiness Ojemekele ◽

Ehimwenma Sheena Omoregie

Keyword(s):

High Fat Diet ◽

Wistar Rats ◽

Leaf Extract ◽

Ldl Cholesterol ◽

Hdl Cholesterol ◽

Negative Control ◽

High Fat ◽

Body Weights ◽

Liver And Kidney ◽

Group 2

Hyperlipidaemia is a well documented risk factor for cardiovascular disease, a major cause of mortality world-wide. Chrysophyllum albidum (white star apple) has several medicinal properties. Ingestion of the fruits has been found to lower lipid levels in the body, due to its high fibre content. Research works have shown that several parts of the plant are highly medicinal. The aim of this study was to evaluate the antihyperlipidaemic property of methanol leaf extract of Chrysophyllum albidum in Wistar rats fed with a high fat diet. Thirty male Wistar rats weighing between 130g-150g were distributed into five groups of six animals each. Group 1 received normal fat diet (NFD). Group 2 received high fat diet (HFD; Grower mash supplemented with 20% beef tallow). Group 3 received HFD and a standard lipid lowering drug, orlistat (20mg/kg body weight, p.o). Animals in group 4 and 5 were fed on HFD and received methanol leaf extract of Chrysophyllum albidum, 250mg/kg and 500mg/kg, p.o respectively. The administration was carried out for 56 days. On the 57th day, animals were sacrificed by cervical dislocation. Organs were harvested and blood samples were collected for biochemical analyses. Results showed that the administration of HFD increased the total cholesterol, LDL-Cholesterol, triglycerides levels and decreased the HDL- cholesterol levels significantly (p <0.05). Simultaneous administration of Chrysophyllum albidum extract significantly (p<0.05) reduced the total cholesterol, LDL-Cholesterol, triglycerides levels and increased HDL- Cholesterol. Final body weights of animals that received Chrysophyllum albidum extract were significantly (p<0.05) lower than final body weights of animals in groups 2 (negative control). Results from Histopathology revealed inflammation of the liver and kidney of rats in group 2 (negative control). On the other hand, rats that received orlistat and Chrysophyllum albidum extract had normal liver and kidney architecture. It was concluded that Chrysophyllum albidum extract showed antihyperlipidemic effect in rats fed with beef tallow supplemented diet.

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Mortality of Sprague Dawley (SD) Rat on Long Term High-Fat Diet (HFD)

International Journal of Advancement in Life Sciences Research ◽

10.31632/ijalsr.20.v04i01.005 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Christianus Rustin ◽

Zulhabri Othman ◽

Muhammad Fakhruddin Irfan Bin Sazali

Keyword(s):

Sudden Death ◽

Chronic Inflammation ◽

High Fat Diet ◽

Cardiac Tissue ◽

Future Research ◽

Sprague Dawley ◽

High Fat ◽

Sd Rat ◽

Sd Rats

Background and Objective: Heart failure is linked with metabolic syndrome due to an unbalanced dietary intake. Previous studies suggest that cardiac dysfunction is related to chronic inflammation due to injury within the heart muscle. This study focused on the examination and sudden death of Sprague Dawley (SD) rat which was on long-term High-Fat Diet (HFD). The outcomes will potentially provide an insight into future research in sudden death due to HFD. Methods: Total of 15 SD rats grouped into ten HFD and five on normal diet (ND); Female SD rats on HFD underwent physical examination, tissue analysis 12 hours post-death using Hematoxylin & Eosin (H&E) followed by morphological assessment and SD rats from ND used as control. Microscopic images processed using ImageJ and data analysis performed in SPSS software. Results: No significant physical trauma prior to death, however on the 60th day SD rat suffered chronic inflammation to cardiac tissues with Lee Index (LI) of 0.30, indicated the occurrence of Myocardial infarction (MI). The average mean between the size area of inflammation and the region on cardiac tissue is (834.19 ± 103.41) and 94.2% of inflammation activities explained by the four random regions of cardiac tissue (R2= 0.942, F(1, 2)= 32.401, p< 0.05), with every one unit increase in the size area of inflammation, the infected region of cardiac tissue is estimated to increase at 0.970 (?= 0.970, p< 0.05). Conclusion: Chronic inflammation resulted in myocardial injuries led to mortality of SD rats largely due to HFD.

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PENGARUH AROMATERAPI MINYAK ATSIRI JAHE TERHADAP KADAR TRIGLISERIDA DAN KOLESTEROL DARAH TIKUS YANG DIBERI PAKAN TINGGI LEMAK

Wahana Peternakan ◽

10.37090/jwputb.v1i2.41 ◽

2018 ◽

Vol 1 (2) ◽

Author(s):

Aji Agung Cahyaji

Keyword(s):

Essential Oil ◽

Total Cholesterol ◽

Cholesterol Level ◽

High Fat Diet ◽

Ldl Cholesterol ◽

Density Lipoprotein ◽

Hdl Cholesterol ◽

Albino Rats ◽

Standard Diet ◽

High Fat

The study aims to determine the effect of ginger (Zingiber officinale) essential oil via inhalation on blood triglyceride, total cholesterol, High Density Lipoprotein (HDL) cholesterol, and Low Density Lipoprotein (LDL) cholesterol level of rats that fed high fat diet. Eighteen albino rats (Rattus norvegicus) were devided into three treatments groups. The treatments were K1 (standard diet) as negative control, K2 (high fat diet) as positive control, and K3 (high fat diet + ginger essential oil inhalation). Blood samples were collected after 5 weeks of treatment period. The result showed the level of triglyceride, cholesterol, and HDL cholesterol at treatment K3 tend to be lower than treatment K2. LDL cholesterol level at treatment K3 show higher result than treatment K2. From the result of this study cocluded that inhalation of ginger essential oil can lowering triglyceride, total cholesterol, and LDL cholesterol level and raise HDL cholesterol level. Keywords: triglyceride, cholesterol, HDL cholesterol, LDL cholesterol, ginger essential oil

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Milk minerals modify the effect of fat intake on serum lipid profile: results from an animal and a human short-term study

British Journal Of Nutrition ◽

10.1017/s0007114513003826 ◽

2013 ◽

Vol 111 (8) ◽

pp. 1412-1420 ◽

Cited By ~ 24

Author(s):

Janne K. Lorenzen ◽

Søren K. Jensen ◽

Arne Astrup

Keyword(s):

Total Cholesterol ◽

High Fat Diet ◽

Ldl Cholesterol ◽

Control Period ◽

Saturated Fat ◽

Hdl Cholesterol ◽

Cholesterol Concentration ◽

Control Group ◽

High Fat ◽

Milk Minerals

Despite a high content of saturated fat, evidence from observational studies indicates that the consumption of dairy products may have a neutral effect or may be inversely associated with the risk of CVD. We aimed to examine whether milk minerals modify the effect of saturated fat on serum lipid profile. We present data from two studies. Study I had a randomised, blinded, parallel design (n 24 pigs) with a 10 d adaptation period during which a high-fat diet was fed to the pigs and a 14 d intervention period during which the same diet either enriched with milk minerals (MM group) or placebo (control group) was fed to the pigs. Study II had a randomised cross-over design (n 9 men) where the subjects were fed either a high-fat diet enriched with milk minerals (MM period) or a regular diet (control period). In both the studies, blood variables were measured before and after the intervention and faecal and urine samples were collected at the end of the dietary periods. The increase in plasma total cholesterol and LDL-cholesterol concentrations but not in HDL-cholesterol concentration was markedly lowered by milk minerals in both the studies. In the animal study, baseline adjusted total cholesterol and LDL-cholesterol concentrations in the MM group were 11 % (P= 0·004) and 13 % (P= 0·03) lower compared with those in the control group after the intervention. Similarly in the human study, baseline adjusted total cholesterol and LDL-cholesterol concentrations were 6 % (P= 0·002) and 9 % (P= 0·03) lower after the MM period compared with those in the control period. HDL-cholesterol concentration was not lowered by milk minerals. These short-term studies indicate that the addition of milk minerals to a high-fat diet to some extent attenuates the increase in total cholesterol and LDL-cholesterol concentrations, without affecting HDL-cholesterol concentration.

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The Effects of TwoLactobacillus plantarumStrains on Rat Lipid Metabolism Receiving a High Fat Diet

The Scientific World JOURNAL ◽

10.1155/2013/135142 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 24

Author(s):

Rastislav Salaj ◽

Jana Štofilová ◽

Alena Šoltesová ◽

Zdenka Hertelyová ◽

Emília Hijová ◽

...

Keyword(s):

Body Weight ◽

Lipid Metabolism ◽

Lactobacillus Plantarum ◽

High Fat Diet ◽

Ldl Cholesterol ◽

Body Weight Gain ◽

Diet Group ◽

High Fat ◽

Probiotic Strains ◽

Total Bile Acids

The aim of our study was to evaluate the effects of the different probiotic strains,Lactobacillus plantarumLS/07 andLactobacillus plantarumBiocenol LP96, on lipid metabolism and body weight in rats fed a high fat diet. Compared with the high fat diet group, the results showed thatLactobacillus plantarumLS/07 reduced serum cholesterol and LDL cholesterol, butLactobacillus plantarumBiocenol LP96 decreased triglycerides and VLDL, while there was no change in the serum HDL level and liver lipids. Both probiotic strains lowered total bile acids in serum. Our strains have no significant change in body weight, gain weight, and body fat. These findings indicate that the effect of lactobacilli on lipid metabolism may differ among strains and that theLactobacillus plantarumLS/07 andLactobacillus plantarumBiocenol LP96 can be used to improve lipid profile and can contribute to a healthier bowel microbial balance.

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Effect of Yogurt Fermented by Lactobacillus Fermentum TSI and L. Fermentum S2 Derived from a Mongolian Traditional Dairy Product on Rats with High-Fat-Diet-Induced Obesity

Foods ◽

10.3390/foods9050594 ◽

2020 ◽

Vol 9 (5) ◽

pp. 594

Author(s):

Won-Young Cho ◽

Go-Eun Hong ◽

Ha-Jung Lee ◽

Su-Jung Yeon ◽

Hyun-Dong Paik ◽

...

Keyword(s):

High Fat Diet ◽

Dairy Product ◽

Hdl Cholesterol ◽

Blood Lipid ◽

Normal Diet ◽

Lactobacillus Fermentum ◽

Sprague Dawley ◽

High Fat ◽

Diet Induced Obesity ◽

Saline Administration

This study aimed to investigate the metabolic effect of yogurt fermented by Lactobacillus fermentum TSI and S2 isolated from a Mongolian traditional dairy product on rats with high-fat-diet-induced obesity. Quality characteristics of yogurt fermented by commercial starter (CON), L. fermentum TSI2 (TSI2 group), L. fermentum S2 (S2 group), and mixed TSI2 and S2 strains at 1:1 (MIX group), were verified. Six-week-old male Sprague-Dawley rats were divided into five groups and administered the following diets: group NOR, normal diet with oral saline administration; group HF, high-fat diet (HD) with oral saline administration; group TSI, HD and L. fermentum TSI-fermented yogurt; group S2, HD and L. fermentum S2-fermented yogurt; and group MIX, HD and MIX-fermented yogurt. After eight weeks, the HD groups displayed significantly increased body weight and fat, serum cholesterol, and abdominal adipose tissue levels. However, serum HDL cholesterol levels were higher, triglyceride levels were lower, and abdominal adipocytes were smaller in the TSI and S2 groups than in the HF group. These results indicate that L. fermentum TSI reduces abdominal fat and improves blood lipid metabolism in HD-induced obese rats.

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Differential sensitivity of chronic high-fat-diet-induced obesity in Sprague-Dawley rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2017-0030 ◽

2018 ◽

Vol 29 (5) ◽

pp. 553-563 ◽

Cited By ~ 2

Author(s):

Shakthi R.K. Devan ◽

Surendar Arumugam ◽

Ganesh Shankar ◽

Suresh Poosala

Keyword(s):

Body Weight ◽

Weight Gain ◽

High Fat Diet ◽

Body Weight Gain ◽

Differential Sensitivity ◽

Sprague Dawley ◽

High Fat ◽

Diet Induced Obesity ◽

High Fat Diets ◽

Fat Diets

AbstractBackgroundThe prevalence of obesity is reported to be increasing owing to the high intake of dietary fat and is a predisposing risk factor with associated complex metabolic syndromes in the human population. Preclinical rodent models play a pivotal role in understanding the pathogenesis of obesity and development of new treatment strategies for humans. High-fat-diet (HFD)-induced rodents are used for chronic obesity models owing to their quick adaptation to high-fat diets and rapid body weight gain and different rats (Wistar Sprague-Dawley and Lewis) have been used by various researchers. However, the selection of appropriate stock contributes to the translation of clinically linked disease phenotypes to preclinical animal models.MethodsThe study was conducted using two commonly used rat stocks Hsd:Sprague-Dawley (SD) and Crl:Charles River (CD) to develop a chronic high-fat-diet-induced obesity model (DIO) to explore the underlying mechanisms of obesity and its utilization in drug discovery and development during preclinical stages. In addition two high-fat diets of different composition were evaluated (D12327; 40% kcal fat and D12492; 60% kcal fat) for their potential to induce obesity using these two stocks.ResultsA differential sensitivity to HFD was observed in body weight gain fat mass composition and obesity-linked symptoms such as impaired glucose tolerance insulin and leptin levels. The comparative research findings of Hsd:SD and Crl:CD rat stocks suggested that Crl:CD rats are more prone to diet-induced obesity and its associated complications.ConclusionsCrl:CD rats were found to be a suitable model for obesity over Hsd:SD when considering the important hallmarks of metabolic disorders that may be utilized for obesity-related research.

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Abstract 18754: Genetic Predisposition to Coronary Artery Disease and Changes in Lipid Profile in Response to 2-year Weight-loss Diet Intervention: The Pounds Lost Trial

Circulation ◽

10.1161/circ.132.suppl_3.18754 ◽

2015 ◽

Vol 132 (suppl_3) ◽

Author(s):

Tiange Wang ◽

Tao Huang ◽

Yan Zheng ◽

George Bray ◽

Frank M Sacks ◽

...

Keyword(s):

Total Cholesterol ◽

Genetic Predisposition ◽

Genetic Risk ◽

High Fat Diet ◽

Ldl Cholesterol ◽

Hdl Cholesterol ◽

High Fat ◽

Low Fat ◽

Diet Intervention ◽

Low Fat Diet

Introduction: Dyslipidemia is a major risk factor of coronary artery disease (CAD), and is affected by interaction between genetic and environmental factors, such as diets. Weight-loss diet intervention has been widely used to improve lipids to mitigate cardiovascular complications of obesity. Hypothesis: We hypothesize that the genetic predisposition to CAD may modify lipids metabolism in response to diets. Methods: We calculated a genetic risk score (GRS) based on 19 CAD-associated single nucleotide polymorphisms in 744 adults from a 2-year diet intervention study: the POUNDS Lost trial. We examined the changes in total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol and triglycerides by the GRS and diet intervention. Results and Conclusions: We found significant interaction between the GRS and dietary fat intake on changes in total cholesterol, HDL and LDL cholesterol (P-interaction=0.02, 0.01 and 0.02, respectively) after the 2-year intervention. Overall, both low- and high-fat diets decreased total cholesterol, LDL cholesterol and triglycerides, but increased HDL cholesterol. At 2 years, in the highest tertile of the GRS, participants eating the low-fat diet showed a greater decrease in total cholesterol and LDL cholesterol than those eating the high-fat diet; whereas participants eating the high-fat diet showed a greater increase in HDL cholesterol than those eating the low-fat diet (all P<0.001). In lower tertiles of the GRS, the difference in lipids changes between the two diet groups was not significant. In conclusion, genetic predisposition to CAD might modify the dietary fat induced lipids changes. Our data suggest that in participants with higher genetic risk, low-fat diet may lower total cholesterol and LDL cholesterol, while high-fat diet may improve HDL cholesterol. And in participants with lower genetic risk, the two diets showed similar effects on improvement of lipid profile.

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