Biosynthesis of Silver and Gold Nanoparticles Using Aqueous Extract from Crinum latifolium Leaf and Their Applications Forward Antibacterial Effect and Wastewater Treatment

Crinum latifolium (CL) leaf is a source of various biologically active compounds such as alkaloid and phenolic compounds, which exhibit anti-inflammatory, antitumor, and antimicrobial effects. In the purpose of expanding applications for the field of bionanotechnology, we report biosynthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) by using aqueous extract from C. latifolium leaf and explore antibacterial activity and catalytic performance for degradation of pollutants. The formation of CL-AgNPs and CL-AuNPs is confirmed and optimized by UV-visible spectroscopy with surface plasmon resonance (SPR) peaks at around 402 and 539 nm, respectively. The spherical CL-AgNPs have an average diameter of 20.5 nm and the multishaped CL-AuNPs possess an average size of 17.6 nm. The actions of four bacterial strains were strongly inhibited by using the CL-AgNPs. Furthermore, the biosynthesized metallic nanoparticles (MNPs) exhibited the excellent catalytic degradation performance of pollutants.

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Green Synthesis of Gold Nanoparticles Using Polianthes tuberosa L. Floral Extract

Plants ◽

10.3390/plants10112370 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2370

Author(s):

Mousa A. Alghuthaymi ◽

Chandrasekaran Rajkuberan ◽

Thiyagaraj Santhiya ◽

Ondrej Krejcar ◽

Kamil Kuča ◽

...

Keyword(s):

Gold Nanoparticles ◽

Metallic Nanoparticles ◽

Antagonistic Activity ◽

Diffusion Method ◽

X Ray Diffraction ◽

Transmission Electron ◽

Potential Applications ◽

Uv Visible ◽

Dose Dependent ◽

Mcf 7

The developments of green-based metallic nanoparticles (gold) are gaining tremendous interest, having potential applications in health care and diagnosis. Therefore, in the present study, Polianthes tuberosa flower filtered extract was used as a reducing and stabilizing agent to synthesize gold nanoparticles (PtubAuNPs). The PtubAuNPs were extensively characterized by UV–visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and X-ray diffraction. The antibacterial activity of PtubAuNPs was determined by the agar well diffusion method; the PtubAuNPs performed extreme antagonistic activity against the tested pathogens. Furthermore, the cytotoxicity of the PtubAuNPs was evaluated in MCF 7 cells by MTT assay. The PtubAuNPs induced toxicity in MCF 7 cells with the least concentration of 100 µg/mL in a dose-dependent method by inducing apoptosis. Overall, the study manifested that PtubAuNPs are a potent nanomaterial that can be employed as an antimicrobial and anticancer agent.

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Optical, Structural, and Antimicrobial Study of Gold nanoparticles Synthesized Using an Aqueous Extract of Mimusops elengi Raw Fruits

Biointerface Research in Applied Chemistry ◽

10.33263/briac106.70857096 ◽

2020 ◽

Vol 10 (6) ◽

pp. 7085-7096

Keyword(s):

Gold Nanoparticles ◽

Aqueous Extract ◽

X Ray Diffraction ◽

X Ray ◽

Green Route ◽

Infra Red ◽

Hazardous Condition ◽

Uv Visible ◽

Mimusops Elengi ◽

Positive Results

Gold nanoparticles (AuNPs) has been synthesized via a green route using an aqueous extract of Mimusops elengi raw fruits. The phytochemicals of the fruit help in stabilizing and capping of the nanoparticles. The formation and stability of the synthesized samples has been explained based on results that we obtain from UV-Visible spectroscopy, Fourier Transform Infra-Red (FTIR) spectroscopy, Dynamic Light Scattering (DLS), X-ray diffraction (XRD), scanning electron microscopy (SEM). Further, the positive results of the antimicrobial test add one more novelty to this work. The axiom of this work is the synthesis of stable AuNPs without using any external stabilizing or reducing agents. The formation of AuNPs with the help of photochemical present in Mimusops elengi is yet another important aspect of this work, which provides an eco-friendly method for the synthesis of AuNPs, which can be used extensively because of its non-hazardous condition.

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M13 Phages Uptake of Gold Nanoparticles for Radio- and Thermal-Therapy and Contrast Imaging Improvement

Applied Sciences ◽

10.3390/app112311391 ◽

2021 ◽

Vol 11 (23) ◽

pp. 11391

Author(s):

Lorenzo Torrisi ◽

Letteria Silipigni ◽

Lubomir Kovacik ◽

Vasily Lavrentiev ◽

Mariapompea Cutroneo ◽

...

Keyword(s):

Gold Nanoparticles ◽

Laser Ablation ◽

Metallic Nanoparticles ◽

Average Diameter ◽

Thermal Therapy ◽

Low Doses ◽

Spherical Nanoparticles ◽

Contrast Imaging ◽

Au Nps ◽

Tumoral Cells

The presented work deals with the uptake of gold nanoparticles (Au NPs) by M13 phages in solutions. In particular, the Au NPs uptake modalities and their localization in the filamentous phages are evaluated and measured. Gold spherical nanoparticles (with an average diameter of the order of 10 nm) are obtained by laser ablation in water with a sodium citrated surfactant. The interest of such application comes from the possibility to employ living biological structures to transport heavy metallic nanoparticles inside cells of tumoral tissues. Indeed, phages have the capability to introduce Au NPs in the proximity to the cell nucleus, increasing the efficiency of DNA destruction in the tumoral cells by employing low doses of ionizing radiation during radiotherapy and hyperthermia treatments. Several analyses and microscopy characterizations of the prepared phages samples embedding gold nanoparticles are presented, demonstrating that the presence of Au NPs increases the phages imaging contrast.

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Green Synthesis of Silver Nanoparticles using Ajuga parviflora Benth and Digera muricata Leaf extract: Their Characterization and Antimicrobial Activity

Revista de Chimie ◽

10.37358/rc.20.10.8349 ◽

2020 ◽

Vol 71 (10) ◽

pp. 50-57

Author(s):

Kamran Mehdi ◽

Wajid Rehman ◽

Obaid-Ur-rahman Abid ◽

Srosh Fazil ◽

Muhammad Sajid ◽

...

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Leaf Extract ◽

State Of The Art ◽

Sem Analysis ◽

Bacterial Strains ◽

Transmission Electron ◽

Protein Amino Acids ◽

Uv Visible ◽

Average Size

The aim of the present study is to search out nontoxic silver nanoparticles synthesized from the leaf extract of two plants Ajuga parviflora Benth and Digera muricata for antimicrobial activity. The plants used in this investigation are rich in alkaloids, flavonoids, steroids, terpenoids, protein, amino acids, carbohydrate, quninones, phenols and tannins. The formation of nanoparticles were confirmed by UV/Visible spectroscopy, peaks at 423nm for Ajuga parviflora Benth and 408nm for Digera muricata. The morphology of the silver nanoparticles was established through state of the art spectroscopic tools. SEM analysis reveals average size of AgNPs 18 nm for Digera muricata and 22 nm for Ajuga parviflora Benth respectively while transmission electron microscopy confirms that AgNPs are spherical in shape. The synthesized nanoparticles were subjected to Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa. The results suggest that the silver nanoparticles have promising activity against all the bacterial strains and can be used an effective bactericides.

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Biosynthesis of Silver and Gold Nanoparticles Using Aqueous Extract of Codonopsis pilosula Roots for Antibacterial and Catalytic Applications

Journal of Nanomaterials ◽

10.1155/2020/8492016 ◽

2020 ◽

Vol 2020 ◽

pp. 1-18

Author(s):

Van-Dat Doan ◽

Bao-An Huynh ◽

Thanh-Danh Nguyen ◽

Xuan-Thang Cao ◽

Van-Cuong Nguyen ◽

...

Keyword(s):

Electron Microscopy ◽

Gold Nanoparticles ◽

Aqueous Extract ◽

Metal Ion ◽

Metallic Nanoparticles ◽

Ion Concentration ◽

Gram Negative ◽

X Ray ◽

Codonopsis Pilosula ◽

Vis Spectroscopy

In this study, biogenic silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were synthesized by a green approach using an aqueous extract from Codonopsis pilosula (CP) roots as a reducing and stabilizing agent. The formation of CP-AgNPs and CP-AuNPs was confirmed and optimized by UV-Vis spectroscopy. The CP-AgNPs and CP-AuNPs obtained under optimum conditions of metal ion concentration, reaction temperature, and reaction time were characterized by high-resolution transition electron microscopy (HR-TEM), selected area electron diffraction (SAED) analysis, field-emission scan electron microscopy (FE-SEM), powder X-ray diffraction (XRD) analysis, Fourier transform infrared (FTIR) spectroscopy, dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS) method. It has been found that the biosynthesized CP-AgNPs and CP-AuNPs were formed in spherical shape with an average size of 10±2.5 nm and 20±3.2 nm, respectively. The biosynthesized metallic nanoparticles exhibited selective bacterial activity against three bacterial strains including two Gram-positive bacteria of Bacillus subtilis and Staphylococcus aureus and one Gram-negative bacteria of Escherichia coli. Meanwhile, there was no antibacterial activity detected toward Gram-negative Salmonella enteritidis. CP-AgNPs and CP-AuNPs also manifested an excellent catalytic performance in the reduction of 1,4-dinitrobenzene, 2-nitrophenol, 3-nitrophenol, and 4-nitrophenol.

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Magnetic Separation and Centri-Chronoamperometric Detection of Foodborne Bacteria Using Antibiotic-Coated Metallic Nanoparticles

Biosensors ◽

10.3390/bios11070205 ◽

2021 ◽

Vol 11 (7) ◽

pp. 205

Author(s):

Mohamed Fethi Diouani ◽

Maher Sayhi ◽

Zehaira Romeissa Djafar ◽

Samir Ben Jomaa ◽

Kamel Belgacem ◽

...

Keyword(s):

Gold Nanoparticles ◽

Rapid Detection ◽

Metallic Nanoparticles ◽

Pathogenic Bacteria ◽

Magnetic Beads ◽

Hydrogen Reduction ◽

Shigella Flexneri ◽

Diagnostic Methods ◽

Bacterial Strains ◽

Efficient Detection

Quality and food safety represent a major stake and growing societal challenge in the world. Bacterial contamination of food and water resources is an element that pushes scientists to develop new means for the rapid and efficient detection and identification of these pathogens. Conventional detection tools are often bulky, laborious, expensive to buy, and, above all, require an analysis time of a few hours to several days. The interest in developing new, simple, rapid, and nonlaborious bacteriological diagnostic methods is therefore increasingly important for scientists, industry, and regulatory bodies. In this study, antibiotic-functionalized metallic nanoparticles were used to isolate and identify the foodborne bacterial strains Bacillus cereus and Shigella flexneri. With this aim, a new diagnostic tool for the rapid detection of foodborne pathogenic bacteria, gold nanoparticle-based centri-chronoamperometry, has been developed. Vancomycin was first stabilized at the surface of gold nanoparticles and then incubated with the bacteria B. cereus or S. flexneri to form the AuNP@vancomycin/bacteria complex. This complex was separated by centrifugation, then treated with hydrochloric acid and placed at the surface of a carbon microelectrode. The gold nanoparticles of the formed complex catalyzed the hydrogen reduction reaction, and the generated current was used as an analytical signal. Our results show the possibility of the simple and rapid detection of the S. flexneri and B. cereus strains at very low numbers of 3 cells/mL and 12 cells/mL, respectively. On the other hand, vancomycin-capped magnetic beads were easily synthesized and then used to separate the bacteria from the culture medium. The results show that vancomycin at the surface of these metallic nanoparticles is able to interact with the bacteria membrane and then used to separate the bacteria and to purify an inoculated medium.

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Green and Synthetic Metallic Nanoparticles - Obtaining, Characterization and Biological Evaluation in Association with Lupeol

Revista de Chimie ◽

10.37358/rc.20.5.8137 ◽

2020 ◽

Vol 71 (5) ◽

pp. 299-304

Author(s):

Flavia Bociort ◽

Adelina Cheveresan ◽

Razvan Dragoi ◽

Alina Heghes ◽

Camelia Szuhanek ◽

...

Keyword(s):

Colon Cancer ◽

Gold Nanoparticles ◽

Cancer Cells ◽

Metallic Nanoparticles ◽

Chemical Characterization ◽

Biological Evaluation ◽

Colon Cancer Cells ◽

Lemon Balm ◽

Physico Chemical ◽

Average Size

The field of metal nanotechnology continues to draw the attention of researchers from different areas due to the outstanding properties of nanoparticles. The main aims of this research were to obtain and to study two types of gold nanoparticles; different obtaining pathways (the chemical synthesis and the biosynthesis based on an aqueous extract of lemon balm) were involved in this research. The chemically synthesized nanoparticles were loaded with lupeol. After a preliminary physico-chemical characterization, the compounds obtained were tested in order to evaluate the effects on the viability of colon cancer cells. The formulation of these new gold nanoparticles with average size between 38 and 82 nm, with a good stability against the tendency to form agglomerations according to the Zeta potential values, was confirmed by UV-Vis spectra; the particles containing lupeol have show the best activity against the colon cancer cells.

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Synthesis of Gold Nanoparticles by using Batch Method and Study its Antibacterial Activity

Baghdad Science Journal ◽

10.21123/bsj.2020.17.2(si).0633 ◽

2020 ◽

Vol 17 (2(SI)) ◽

pp. 0633

Author(s):

Ahmed Mohammed et al.

Keyword(s):

Gold Nanoparticles ◽

Dilution Method ◽

X Ray Diffraction ◽

X Ray ◽

E Coli ◽

Inhibition Concentration ◽

Uv Visible ◽

Average Size ◽

Broth Dilution ◽

Initial Heating

The research involves preparing gold nanoparticles (AuNPs) and studying the factors that influence the shape, sizes and distribution ratio of the prepared particles according to Turkevich method. These factors include (reaction temperature, initial heating, concentration of gold ions, concentration and quantity of added citrate, reaction time and order of reactant addition). Gold nanoparticles prepared were characterized by the following measurements: UV-Visible spectroscopy, X-ray diffraction and scanning electron microscopy. The average size of gold nanoparticles was formed in the range (20 -35) nm. The amount of added citrate was changed and studied. In addition, the concentration of added gold ions was changed and the calibration curve was calculated used to estimate Au ions in different samples. The effectiveness of gold nanoparticles prepared according to Turkevich method was studied as antibacterial agents against E. coli bacteria. The minimum inhibition concentration of gold nanoparticles that inhibit bacterial growth was calculated using the broth dilution method, which is based on several dilutions to determine the inhibition concentration.

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Fruit Biowaste Mediated Green Route Approach Silver Nanopartilces - As Antibacterial Material

Kongunadu Research Journal ◽

10.26524/krj307 ◽

2019 ◽

Vol 6 (2) ◽

pp. 81-86

Author(s):

Ranjith Kumar R ◽

Manikantan J ◽

Balaganesh A.S ◽

Dinesh K.P.B ◽

Chandar Shekar B

Keyword(s):

Green Chemistry ◽

Aqueous Extract ◽

Metallic Nanoparticles ◽

Nitrate Solution ◽

Antibacterial Properties ◽

Silver Nitrate Solution ◽

Sem Image ◽

Vis Spectroscopy ◽

Antibacterial Material ◽

Average Size

Environmental free approach or green chemistry synthesis of metallic nanoparticles has become new growing branch of nanobiotehnology. In this present work a simple and environmental free biosynthesis silver nanoparticles (AgNPs) were prepared using Musambi Peels (MPs) aqueous extract as the reducing agent guided by the principles of green chemistry. The fruit waste aqueous extract was challenged with silver nitrate solution for the production of AgNPs in room temperature. The crystalline phase and morphology of AgNPs were determined from UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectra, X-raydiffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS).The UV-Vis spectrum indicated that the surface plasmon broad peak was observed nearby 450 nm throughout the reaction 30min-24h. XRD spectrum revealed that the average size of biowaste mediated AgNPs obtained approximately 46 nm by using the Debye-Scherrer equation. SEM image of AgNPs showed uniformly distributed on the surface of the cell with high agglomeration. EDS analysis revealed that the presence of silver was confirmed from the Ag peaks at 2.8-3.7 keV. In addition, the biowaste mediated AgNPsloaded disk were tested for antibacterial properties against Escherchia coli and Staphylococcus aureus and found that the obtained metallic AgNPs have been good antibacterial material for biological applications.

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Synergistic antibacterial activity of herbal extracts with antibiotics on bacteria responsible for periodontitis

The Journal of Infection in Developing Countries ◽

10.3855/jidc.14904 ◽

2021 ◽

Vol 15 (11) ◽

pp. 1685-1693

Author(s):

Shahabe Abullais Saquib ◽

Nabeeh Abdullah AlQahtani ◽

Irfan Ahmad ◽

Suraj Arora ◽

Shaik Mohammed Asif ◽

...

Keyword(s):

Antibacterial Activity ◽

Plant Extracts ◽

Bacterial Resistance ◽

Antibacterial Effect ◽

Punica Granatum ◽

Biologically Active ◽

Diffusion Method ◽

Bacterial Strains ◽

Periodontopathic Bacteria ◽

Favorable Conditions

Introduction: Development of bacterial resistance and antimicrobial side-effect has shifted the focus of research toward Ethnopharmacology. A biologically active compound derived from the plants may increase the effectiveness of antibiotic when used in combination. The present study aims to determine the synergistic antibacterial effect of ethanolic extracts of Punica granatum (pericarp), Commiphora molmol, Azadirachta indica (bark) in combination with amoxicillin, metronidazole, tetracycline, and azithromycin on periodontopathic bacteria: Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans. Methodology: Periodontopathic bacterial strains were isolated from the plaque sample that was collected from periodontitis patients and grown under favorable conditions. Susceptibility of bacteria to the antibiotics and extracts was determined by disc diffusion method by measuring the diameter of the inhibition zones. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts were evaluated against each bacterium. Synergistic effect of plant extract in combination with antibiotics was tested against each bacterium by measuring the diameter of zone of inhibition (ZOI). Results: Findings revealed that all plant extracts exhibited an inhibitory effects on the proliferation and growth of periodontopathic bacteria. The maximum antibacterial effect was exhibited by C. molmol on P. gingivalis (ZOI = 20 ± 0.55 mm, MIC = 0.53 ± 0.24 mg/mL and MBC = 5.21 ± 1.81 mg/mL) (p < 0.05), meanwhile, no antibacterial activity was exhibited by P. granatum on T. forsythia. Synergistic antibacterial effect was recorded when plant extracts were used in combination with antibiotics. The best synergism was exhibited by P. granatum with amoxicillin against A. actinomycetemcomitans (24 ± 1.00 mm) (p < 0.05). Conclusions: The synergistic test showed significant antibacterial activity when plant extracts were combined with antibiotics against all the experimented bacteria.

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