Increased Serum Levels of IFN-γ, IL-1β, and IL-6 in Patients with Alopecia Areata and Nonsegmental Vitiligo

Alopecia areata (AA) and vitiligo are both common skin diseases of autoimmune origin. Both alopecia areata and vitiligo have shown to be affected by oxidative stress. The present work is aimed at evaluating and comparing the serum proinflammatory cytokine levels in AA and nonsegmental vitiligo (NSV). A cross-sectional study was conducted of 33 patients with AA, 30 patients with NSV, and 30 healthy controls. Serum levels of interferon γ (IFN-γ), interleukin- (IL-) 1β, and IL-6 were determined quantitatively by ELISA method. Our analysis identified a signature of oxidative stress associated with AA and NSV, characterized by elevated levels of IFN-γ (AA: p=0.007283; NSV: p=0.038467), IL-1β (AA; NSV: p≤0.001), and IL-6 (AA; NSV: p≤0.001). IL-6 was also significantly increased in NSV patients in comparison with AA patients (p=0.004485). Our results supported the hypothesis that oxidative stress may play a significant role in promoting and amplifying the inflammatory process both in AA and vitiligo. The complex understanding of both disease etiopathogenesis involves interrelationships between oxidative stress and autoimmunity. The clinical study registration number is RNN/266/16/KE.

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Serum Cytokine Levels and Their Relation to Clinical Features in Patients with Autoimmune Liver Diseases

Journal of Immunology Research ◽

10.1155/2017/9829436 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 9

Author(s):

Dilyara Akberova ◽

Andrei P. Kiassov ◽

Diana Abdulganieva

Keyword(s):

Liver Diseases ◽

Serum Levels ◽

Cross Sectional Study ◽

Overlap Syndrome ◽

Sectional Study ◽

Serum Cytokine ◽

Cross Sectional ◽

Autoimmune Liver Diseases ◽

Cytokine Levels ◽

Adjusted Logistic Regression

Serum cytokine levels were explored in a combined group of patients with autoimmune liver diseases (AILDs) and separately in patients with autoimmune hepatitis (AIH) and overlap syndrome. Overall, 60 patients with AILD, among them 32 patients with AIH and 28 patients with overlap syndrome, were included in the cross-sectional study. Serum cytokine levels were measured at baseline and compared to those of 21 healthy controls. Patients with AILD had significantly higher levels of IL-6 (0.70 (range 0.17–99.86) in patients with AILD compared to 0.40 (range 0.14–2.65) in controls,p<0.01), IL-8 (1.66 (0.45–34.58) versus 0.53 (0.35–2.38), resp.,p<0.01), and TNF-α(2.61 (0.23–120.88) versus 1.65 (0.21–7.54), resp.,p<0.01). Adjusted logistic regression analysis revealed a pronounced relation of IL-8 and AILD, 48.36 (3.63–643.60), as well as AIH, 18.54 (1.08–318.54), and overlap syndrome, 23.85 (2.37–240.23), while the associations between the level of other cytokines and AILD were assessed as nonsignificant. In the language of absolute numbers, the increase of IL-8 serum level by 1 pg/mL had increased the chance for a patient to find himself in a group of AILD by 48.36 times. Also, high IL-8 serum levels were strongly related to clinical parameters.

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Evaluation of Serum Levels of Inflammation, Fibrinolysis and Oxidative Stress Markers in Coronary Artery Disease Prediction: A Cross-Sectional Study

Arquivos Brasileiros de Cardiologia ◽

10.5935/abc.20190214 ◽

2019 ◽

Cited By ~ 1

Author(s):

Iran Castro ◽

Hugo Fontana Filho

Keyword(s):

Oxidative Stress ◽

Serum Levels ◽

Cross Sectional Study ◽

Disease Prediction ◽

Oxidative Stress Markers ◽

Sectional Study ◽

Cross Sectional ◽

Stress Markers ◽

Artery Disease ◽

And Oxidative Stress

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Task shifting in dermatology: Are nurses prepared and willing?

Our Dermatology Online ◽

10.7241/ourd.20221.4 ◽

2022 ◽

Vol 13 (1) ◽

pp. 22-27

Author(s):

Kavita Kavita ◽

Hitaishi Mehta ◽

Sandhya Ghai ◽

Aarti Garg ◽

Tarun Narang

Keyword(s):

Skin Diseases ◽

Task Shifting ◽

Cross Sectional Study ◽

Attitude Scale ◽

Cross Sectional ◽

Low Level ◽

High Knowledge ◽

Common Skin ◽

Level Of Knowledge ◽

Skin Conditions

Background: The high burden of skin diseases and the shortage of dermatologists are significant challenges in providing care to millions of people with skin diseases. Task shifting to nurses is a viable option for the delivery of dermatologic care in resource-poor settings. Satisfactory knowledge and a positive attitude are crucial for nurses to undertake the task of managing common skin diseases. This study aimed to investigate the knowledge of registered nurses and their attitude toward common skin conditions. Methods: In this descriptive, cross-sectional study, a total of 187 nurses were included from a nurses training institute by total enumeration sampling. A knowledge questionnaire and five-point Likert type-attitude scale were developed, validated, and employed to collect data. Written informed consent was obtained from the participants after approval from the institute ethics committee. Results: The mean of the knowledge scores were 10.7 ± 2.2. Nearly two thirds (62%) of the subjects demonstrated a low level of knowledge, while the remaining 38% showed a moderate level. There was no participant in the high-knowledge category. A majority of the nurses demonstrated a favorable attitude toward learning and undertaking the task of managing common skin conditions. Conclusion: We observed a low level of knowledge on diagnosing and managing common skin conditions. We recommend incorporating the relevant concepts of common skin conditions in the nursing curriculum with an emphasis on continuing education.

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Expression Levels of Inflammatory and Oxidative Stress-Related Genes in Skin Biopsies and Their Association with Pityriasis Alba

Medicina ◽

10.3390/medicina56070359 ◽

2020 ◽

Vol 56 (7) ◽

pp. 359

Author(s):

Margarita L. Martinez-Fierro ◽

Griselda A. Cabral-Pacheco ◽

Idalia Garza-Veloz ◽

Andrés E. Campuzano-García ◽

Alma P. Díaz-Alonso ◽

...

Keyword(s):

Oxidative Stress ◽

Tissue Expression ◽

Cross Sectional Study ◽

Healthy Skin ◽

Cross Sectional ◽

Expression Levels ◽

Over Expression ◽

Common Skin ◽

Skin Biopsies ◽

Stress Related Genes

Background and objectives: Pytiriasis alba (PA) is a common skin disorder which affects 80% of children between six and 16 years. The etiology of PA is unclear, but hypo-pigmented patches in photo-exposed zones characterize the disease. Because the high ultraviolet exposition of the skin promotes an acute inflammatory response and an increase of oxidative stress (OS), this study aimed to evaluate the expression levels of inflammatory and OS-related genes in skin biopsies, and their association with PA. Materials and Methods: A cross-sectional study was carried out. Skin biopsies of the lesion sites and healthy skin (controls) from 16 children with PA were evaluated. The tissue expression of IL-4, IL-6, IL-17A, TNFα, INFγ, IL-1β, SOD1, and HMOX1 was analyzed by qRT-PCR, using SYBR Green and glyceraldehyde-3-phosphate dehydrogenase gene as the endogenous control. Results: There were differences in the ΔCq values of HMOX1, SOD1, IL-6, and IFNγ between tissue with lesions and healthy skin (p < 0.05). Compared with healthy skin, IL-6, IFNγ, HMOX1, and SOD1 were predominantly under-expressed in the lesion sites. However, 25% of skin biopsies with lesions showed over-expression of these four genes. Positive correlations between the expression of IL-6 and HMOX1, SOD1, and IFNγ (p < 0.05) were also observed. Conclusions: Our results suggest the presence of molecular stages of PA, defined according to the over-expression (first stage) or under-expression (second stage) of the HMOX1, SOD1, IL-6, and IFNγ genes in abnormal skin tissue. These findings may have implications for the selection of treatment for PA-related lesions.

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Pattern of skin diseases in patients attended OPD of Dermatology and Venereology in a tertiary care teaching hospital in Bangladesh

Bangladesh Journal of Physiology and Pharmacology ◽

10.3329/bjpp.v29i1-2.20061 ◽

2014 ◽

Vol 29 (1-2) ◽

pp. 8-11

Author(s):

Abul Kalam Mohammad Yousuf ◽

Yasmin Joarder ◽

Ayasha Aktar ◽

Md. Nazmul Hoq ◽

Mushfika Rahman ◽

...

Keyword(s):

Skin Diseases ◽

Acne Vulgaris ◽

Tertiary Care ◽

Cross Sectional Study ◽

Personal Hygiene ◽

College Hospital ◽

Cross Sectional ◽

Economic Class ◽

Medical College ◽

Common Skin

Background: Skin problems are one of the most common health problems seen in the developing countries. Objective: To assess the pattern of skin diseases most commonly encountered in Dermatology & Venereology Outdoor Patient Department (OPD) of Northern International Medical college Hospital (NIMCH). Materials and Methods: This cross sectional study was carried out in the Dermatology & Venereology OPD of NIMCH, Dhanmondi, Dhaka, Bangladesh, during the period of January 2012 to December 2012. A total of 1169 patients (511male and 658 female) aged 10-44 years, attended in the OPD in which 969 patients (450 male and 519 female) were found to have dermatological problems. Data were analyzed by using SPSS soft-ware version 18. Results: The pattern of skin diseases was higher in females (53.6%) than in males (46.4%). The most common disorders seen in outpatient department is eczema (23.7%) followed by fungal infection (15.5%), scabies (13.4%) and acne vulgaris (11.9%). A remarkable part (41.4%) of the patients was illiterate. Majority of the patients (67.6%) belonged to poor socio-economic class. 61.4 % patients were residing in overcrowded homes and 53% patients were not maintained personal hygienic standards Conclusion: This study gives a fair picture of pattern of common skin diseases in urban Dhanmondi and its surrounding locality, Dhaka. From this study, it can be concluded that better health education, maintaining personal hygiene, improvement in the standard of living, proper case diagnosis and proper treatment may remain of importance in managing common skin diseases. http://dx.doi.org/10.3329/bjpp.v29i1-2.20061 Bangladesh J Physiol Pharmacol 2013; 29(1&2) : 8-11

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Profil penyakit kulit pada pelajar sekolah asrama di Kabupaten Magelang, Jawa Tengah

Journal of Community Empowerment for Health ◽

10.22146/jcoemph.38312 ◽

2019 ◽

Vol 1 (2) ◽

pp. 79

Author(s):

Tuntas Rayinda ◽

Devi Artami Susetiati ◽

Sri Awalia Febriana

Keyword(s):

Skin Diseases ◽

Acne Vulgaris ◽

Boarding Schools ◽

Cross Sectional Study ◽

Pityriasis Versicolor ◽

Hormonal Changes ◽

Sectional Study ◽

Cross Sectional ◽

Different Types ◽

Common Skin

Individuals who live in boarding schools often experience various skin diseases, both infection and non-infectious because of exposure to various risk factors such as hormonal changes, poor hygiene and sanitation, and dense living quarters. To date, data on the prevalence of disease in adolescents in boarding schools are still very limited. This study is an observational cross-sectional study that aims to describe skin diseases that often occur in adolescents who live in boarding schools. Surveys and clinical examinations are carried out by dermatologist. Based on a survey of 1,250 students aged 10 - 16 years, 1,073 students (85.8%) had at least one skin disease. A total of 1,073 cases from 27 different types of skin diseases were found in these students. The five most common skin diseases are dermatophytosis, scabies, acne vulgaris, ecthyma, and pityriasis versicolor. Education, periodic surveys, mass treatment, and interventions to improve hygiene and living conditions are key to success in the management of skin diseases in students living in boarding schools.

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Prevalence of Common Skin Diseases and Their Associated Factors among Military Personnel in Korea: A Cross-sectional Study

Journal of Korean Medical Science ◽

10.3346/jkms.2012.27.10.1248 ◽

2012 ◽

Vol 27 (10) ◽

pp. 1248 ◽

Cited By ~ 20

Author(s):

Jung Min Bae ◽

Beomman Ha ◽

Hongsun Lee ◽

Chang Keun Park ◽

Hyun Joon Kim ◽

...

Keyword(s):

Military Personnel ◽

Associated Factors ◽

Skin Diseases ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Common Skin

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Identification of Sarcoptes scabiei by Clinical Examination and Follow-up Examination in Medan City, Indonesia

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.7692 ◽

2021 ◽

Vol 9 (B) ◽

pp. 1633-1636

Author(s):

Afrida Aryani Nasution ◽

Imam Budi Putra ◽

Mutiara Indah Sari

Keyword(s):

Skin Diseases ◽

Clinical Symptoms ◽

Delta Wing ◽

High Sensitivity ◽

Sarcoptes Scabiei ◽

Cross Sectional Study ◽

Cross Sectional ◽

Common Skin ◽

Reverse Primer

Background: Scabies is a disease caused by the mite Sarcoptes scabiei var. hominis. In Indonesia, scabies ranks third out of the 12 most common skin diseases. In terms of disease screening, direct visualization of dermatitis from mites and microscopy of skin scrapings is less sensitive. PCR and dermoscopy examinations have a high sensitivity value to Sarcoptes mites. Aims: This study aims to identify Sarcoptes scabiei mites between clinical symptoms and supporting examinations, namely PCR and dermoscopy methods. Methods: This research is a cross-sectional study, with descriptive analytics. The number of samples of 50 people who met the inclusion criteria was examined by microscopic examination, dermoscopy, and PCR. We state it to be positive if we found scabies mites or their eggs on microscopy, delta wing sign, or jet wet contrail on dermoscopy and there is a 135bp DNA band on PCR. Results: 50 samples diagnosed with scabies based on cardinal sign of scabies, gender were 80% male and 20% female with an average age of 14 years. Based on the location of the rash, the most rashes were between the fingers and toes, each 26% and the least on the head as much as 2%. Based examination tools, no Sarcoptes scabiei mites were found through microscopic and dermoscopic examination, while the PCR examination found 12 positive samples of scabies. Conclusion: PCR examination is very sensitive and specific even in very small quantities, with the fore primer SSUDF and the reverse primer SSUDR. Further research is needed to assess the sensitivity and specificity of dermoscopy and PCR in diagnosing scabies.

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Pattern of skin diseases in patients attending OPD of selected upazilla health complex, Bangladesh

Z H Sikder Women’s Medical College Journal ◽

10.47648/zhswmcj.2020.v0202.01 ◽

2020 ◽

Vol 2 (Number 2) ◽

pp. 3-6

Author(s):

Chinmay Biswas ◽

Shila Rani Das

Keyword(s):

Skin Diseases ◽

Social Economic ◽

Parasitic Infection ◽

Cross Sectional Study ◽

Cross Sectional ◽

Different Types ◽

Common Skin ◽

Study Population ◽

Infectious Skin Diseases ◽

Diagnostic Importance

Skin diseases differ in different countries, and within various regions of a country depending on social, economic, racial and environmental factors. Many patients have reported various patterns of skin diseases in different countries. It was a descriptive type of cross sectional study conducted at outpatient department (OPD) of Dhamrai upazilla health complex to know the pattern of skin diseases during the period of June 2018 to December 2018. Total 2000 patients at any age and sex who attended in the OPD of the hospital with different types of skin disorders/diseases were selected as study population. Among the patients 27.05% were less than 10 years of age and 56.04% were female, 45.05% patients were illiterate, 57.03% had monthly family oncome more than Tk 15000 and 40.89% were housewife, 20.01% were students. The diagnosis was made on clinical basis as an expert dermatologist. Lab. investigations were restricted to the cases where they carried diagnostic importance. Majority (57%) were suffering from some infectious among them 33.02% had parasitic infection, 28.3% suffering from fungal infection, 20.1% bacterial infection and 43% from some non-infectious skin diseases among them 37.79% had eczema, 17.87% had papulosquamous disease, 14.25% had acne, 14.01% had urticaria, 8.7% had vitiligo. Infectious skin diseases were the most common skin disease seen in our study, followed by dermatological infections. The emerging challenges for dermatologists are to prevent and reduce these skin diseases.

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