scholarly journals The Antimicrobial Effect of Pomegranate Peel Extract versus Chlorhexidine in High Caries Risk Individuals Using Quantitative Real-Time Polymerase Chain Reaction: A Randomized Triple-Blind Controlled Clinical Trial

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Benoy Jacob ◽  
Nivedhitha Malli Sureshbabu ◽  
Manish Ranjan ◽  
Aishwarya Ranganath ◽  
Riluwan Siddique
Keyword(s):  
Clinical Trial ◽  
Control Group ◽  
Caries Risk ◽  
Chain Reaction ◽  
Pomegranate Peel ◽  
Oral Rinse ◽  
High Caries Risk ◽  
Polymerase Chain ◽  
Pomegranate Peel Extract ◽  
Peel Extract

The aim of the present study was to compare the antibacterial effectiveness of chlorhexidine and PPE oral rinse on S. mutans, Lactobacilli, and Veillonella, in clinical salivary samples of patients with advanced stages of dental caries at baseline and two and four weeks with PCR technique. This triple-blind randomized clinical trial involved 60 high caries risk adult patients, 19–59 years of age, randomly allocated into two groups of 30 subjects each. The intervention group received pomegranate peel extract mouthwash, whereas the control group received chlorhexidine mouthwash. Unstimulated pooled saliva was collected from the floor of the mouth before and after the intervention. The quantitative real-time polymerase chain reaction was employed to analyze the bacterial copies of each salivary sample at baseline and two and four weeks. The significance level was fixed at 5% (α = 0.05). Overall comparison of antimicrobial effectiveness across both groups revealed insignificant outcomes. The control group evinced a significant reduction in S. mutans between a specific time, i.e., baseline and 4 weeks ( p = 0.043 ). PPE oral rinse as a natural product or ecological alternative was effective in disrupting activity across all microorganisms tested in this triple-blind RCT; however, the nutraceutical, when compared to chlorhexidine, was not as effective against S. mutans.

scholarly journals Pomegranate Peel Extract Is a Potential Alternative Therapeutic for Giardiasis

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 705
Author(s):  
Asmaa M. El-Kady ◽  
Iman A. M. Abdel-Rahman ◽  
Samer S. Fouad ◽  
Khaled S. Allemailem ◽  
Taghrid Istivan ◽  
...  
Keyword(s):  
Diarrheal Disease ◽  
Apoptotic Cell ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Control Group ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Multiple Drugs ◽  
Peel Extract

Giardiasis is a major diarrheal disease affecting approximately 2.5 million children annually in developing countries. Several studies have reported the resistance of Giardia lamblia (G. lamblia) to multiple drugs. Therefore, identifying an effective drug for giardiasis is a necessity. This study examined the antiparasitic effect of Punica granatum (pomegranate) and evaluated its therapeutic efficacy in rats infected with G. lamblia. In vitro study showed high efficacy of pomegranate peel ethanolic extract in killing G. lamblia cysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number of G. lamblia cysts and trophozoites in feces and intestine respectively. Interestingly, the number of G. lamblia trophozoites and cysts were significantly lower in the pomegranate extract-treated group compared to the metronidazole-positive control group. Moreover, pomegranate extract treatment significantly induced nitric oxide (NO) and reduced serum IL-6 and TNF-α, compared to infected untreated rats. Histological and scanning electron microscopy (SEM) examination of the jejunum and duodenum of pomegranate extract-treated animals confirmed the antiparasitic effect of the extract, and demonstrated the restoration of villi structure with reduction of villi atrophy, decreased infiltration of lymphocytes, and protection of intestinal cells from apoptotic cell death. In conclusion, our data show that the pomegranate peel extract is effective in controlling G. lamblia infections, which suggests that it could be a viable treatment option for giardiasis.

scholarly journals Krim ekstrak kulit delima merah (Punica granatum) menghambat peningkatan jumlah melanin sama efektifnya dengan krim hidrokuinon pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Eva R. Siahaan ◽  
Wimpie Pangkahila ◽  
A A G P Wiraguna
Keyword(s):  
Guinea Pigs ◽  
Punica Granatum ◽  
Control Group ◽  
Cavia Porcellus ◽  
Pomegranate Peel ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract: As the outer organ of the body, skin becomes the main target of environmental influences, particularly the UVB rays. Based on analysis of phytochemicals, red pomegranate peel extract contains antioxidants such as flavonoids, phenols, tannins, and vitamin C which are able to prevent increased skin melanin by inhibiting the actions of tyrosinase. This study was aimed to prove that application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in female guinea pigs (Cavia porcellus) exposed to UVB as well as to prove that the 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin. This was a true experimental study using post test only control group design. Subjects were divided into three groups, with 10 guinea pigs each group. Group 1, the control group, was treated with UVB exposure and basic cream. Group 2 was treated with UVB exposure and 4% hydroquinone cream. Group 3 was treated with UVB and 8% red pomegranate peel extract cream. A total of 390 mJ/cm UVB dosage was given for 2 weeks. Histopahtologic slides were stained with Masson-Fontana. The amount of melanin area colored in black was evaluated by the percentage of pixel areas of melanin compared with pixel areas of all epidermal tissues. The results showed that the highest amount of melanin was in group 1 (18.13±4.76%), followed by group 3 (1.876±0.73%), and group 2 as the lowest (0.67±0.291%). There was a significant difference between control group and group 2 as s well as group 3 (P <0.05). There was no significant difference between group 2 and group 3 (P >0.05). Conclusion: Application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in guinea pigs (Cavia porcellus) exposed to UVB. The 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin.Keywords: red pomegranate peel extract, melanin, skin, UVBAbstrak: Sebagai organ terluar dari tubuh, kulit menjadi target utama dari pengaruh lingkungan, terutama oleh sinar UVB. Berdasarkan analisis fitokimia, ekstrak kulit delima merah memiliki kandungan antioksidan seperti flavonoid fenol, tanin, dan vitamin C yang mampu mencegah peningkatan jumlah melanin dengan cara menghambat kerja enzim tirosinase. Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak kulit delima merah (Punica granatum) 8% dalam menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB dan krim ekstrak kulit delima merah (Punica granatum) 8% memiliki efektivitas yang sama dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin tersebut. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subyek penelitian dibagi menjadi tiga kelompok, masing-masing berjumlah 10 ekor marmut betina. Kelompok 1 (kontrol) diberikan pajanan sinar UVB dan krim dasar; kelompok 2 diberikan paparan sinar UVB dan krim hidrokuinon 4%; dan kelompok 3diberikan pajanan sinar UVB dan krim ekstrak kulit delima merah 8%. Dosis total UVB 390 mJ/cm2 yang diberikan selama 2 minggu. Sediaan histopatologik jaringan kulit menggunakan pewarnaan Masson-Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan bahwa jumlah melanin paling tinggi terdapat pada kelompok 1 (18,13±4,76%), diikuti kelompok 3 (1,876±0,73%), dan paling rendah pada kelompok 2 (0,67±0,291%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05) sedangkan antara kelompok 2 dan 3 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P >0,05). Simpulan: Krim ekstrak kulit delima merah (Punica granatum) 8% dapat menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina. Krim ekstrak kulit delima merah (Punica granatum) 8% sama efektif dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin pada kulit marmut.Kata kunci: kulit delima merah, melanin, kulit, UVB, marmut betina.

scholarly journals AMELIORATIVE EFFECTS OF PUNICA GRANATUMON RENAL TUBULAR NECROSIS INDUCED BY NANDROLONE DECANOATE IN MICE MODEL

2021 ◽  
Vol 71 (4) ◽  
pp. 1248-51
Author(s):  
Humaira Ali ◽  
Liaqat Ali ◽  
Sadia Shaukat ◽  
Maria Yousaf ◽  
Faiza Umbreen ◽  
...  
Keyword(s):  
Body Weight ◽  
Control Group ◽  
Nandrolone Decanoate ◽  
Oral Gavage ◽  
Pomegranate Peel ◽  
Tubular Necrosis ◽  
Group A ◽  
Renal Tubular ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Objective: To observe the effects of nandrolone decanoateon renal tubules and its protection by punicagranatum (pomegranate) in mice. Study Design: Lab based experimental study. Place and Duration of Study: One year in Anatomy department, Army Medical College, Rawalpindi, in association with National Institution of Health, Islamabad. Methodology: Forty healthy BALB/c mice of both sexes with weight range of 2 5-30gms were equally disseminated into four groups, A as control while B, C and D as trial groups. Three trial groups were inoculated Nandrolone Decanoate 1mg per 100gm of body weight, through intramuscular injections in the hind limb, once weekly for eight weeks. Pomegranate nector was administered in animals of trial group C (3ml per kg of body weight) en route oral gavage tube daily, whereas animals in trial group D was administered pomegranate peel extract (200mg per kg body weight) via oral gavage tube daily for eight weeks. Evaluation of the outcomes of trial groups B, C & D was done amongst them and with control group A. Results: In nandrolone decanoate injected experimental group B, tubular necrosis were appreciated in comparison with control group A (p˂ 0.001), and showed statistical improvement when evaluation was done with pomegranate nectar and pomegranate peel extract treated trial groups C (0.001) and D (p=0.001), correspondingly. Conclusion: Punicagranatum in two forms, as nectar and peel extract, has almost identical curative effects on steroid administered renal tubular mutilation.

scholarly journals Determination of antioxidant and antimicrobial effects of pomegranate peel extract in trout burgers stored at cold temperatures (4±1°C)

2020 ◽  
Vol 37 (4) ◽  
pp. 415-422
Author(s):  
Ilknur Uçak
Keyword(s):  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Control Group ◽  
Pomegranate Peel ◽  
Cold Temperatures ◽  
Pomegranate Peel Extract ◽  
Peel Extract ◽  
Total Yeast

Recently, it has become a common practice to utilize food processing waste as natural additives in food products by evaluating their antioxidant and antimicrobial activities. Pomegranate peel is also an important by-product involved in the pomegranate processing industry and has strong antioxidant and antimicrobial properties. Therefore, the aim of this study is to examine the quality changes of trout burgers prepared with pomegranate peel extract during the refrigerated (4±1°C) storage. For this purpose, peroxide value, thiobarturic acid (TBARs), total aerobic mesophilic bacteria (TAMB) count, total psyschophilis bacteria (TPB) count, total coliform bacteria, total yeast-mold, total lactic acid bacteria (LAB) count and sensory quality parameters were evaluated in trout burgers. Peoxide value was found to be 2.75 meq O2/kg at the beginning with increasing in all groups and were observed significantly lower in the groups prepared with pomegranate peel extract than the control group (24.85 meq O2/kg). TBARs value of trout fillet was 0.07 mg MDA/kg and increased in all groups until the end of storage. The lowest values compared to the control group were found in the group prepared with 1% pomegranate peel extract. At the end of storage, TBARs values of the control, groups enriched with 0.5% and 1% pomegranate peel extract reached 2.78, 2.32 and 2.25 mg MDA/kg, respectively. It has been determined that pomegranate peel extract has a suppressive effect on TAMB, TPB, total coliform, total yeast-mold and LAB growth in trout burgers. As a result of sensory evaluations, pomegranate peel extract has been observed to extend the shelf life of trout burgers by 6 days compared to the control group. All these data revealed that pomegranate peel extract can be used as an alternative natural additive in trout burgers.

scholarly journals The effect of red pomegranate (Punica ganatum) peel extract on leukocyte number and type in rats exposed with mobile phone electromagnetic radiation

2011 ◽  
Vol 9 (2) ◽  
pp. 55-61
Author(s):  
MULKI RAKHMAWATI ◽  
ISNA QADRIYATI ◽  
LILIK WIJAYANTI
Keyword(s):  
Electromagnetic Radiation ◽  
Mobile Phone ◽  
Punica Granatum ◽  
Exposed Group ◽  
T Test ◽  
Control Group ◽  
Pomegranate Peel ◽  
Leukocyte Number ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Rakhmawati M, Qadriyati I, Wijayanti L. 2011. The effect of red pomegranate (Punica granatum) peel extract on leukocyte number and type in rats exposed with mobile phone electromagnetic radiation. Biofarmasi 9: 55-61. This research aimed to examine the effect of red pomegranate (Punica granatum) peel extract on leukocyte number and type in rats exposed with mobile phone electromagnetic radiation. This study was a laboratory experimental post-test only control group design. The subjects used were 32 male rats, divided into 4 groups: (i) control group, (ii) mobile phone electromagnetic radiation-exposed group for 4 hour/day for 14 days, (iii) mobile phone electromagnetic radiation-exposed group for 4 hour/ day for 14 days with red pomegranate peel extract 50 mg/kg weight body in pre and during exposed, and (iv) mobile phone electromagnetic radiation-exposed group for 4 hour/day for 14 days with red pomegranate peel extract 50 mg/kg body weight pre, during and post-exposed. After 41 days, blood was collected in a clean tube with EDTA from orbital sinus of rats. Blood was used for leukocyte number and type in Pathology Clinic Laboratory, Faculty of Medicine, Sebelas Maret University. The data obtained were statistically analyzed with independent t-test by using SPSS Program for Microsoft Windows release 16.0 with a significance level at p<0.05. The results showed that red pomegranate peel extract decrease the leukocyte number than only exposed to mobile phone electromagnetic radiation group. The treatment of red pomegranate peel extract decreased for eosinophil, lymphocyte and monocyte, while for neutrophil, the treatment of red pomegranate peel extract showed increase than only exposed with mobile phone electromagnetic radiation group. Statistical analysis with independent t-test showed that the result was significant between group 1 and 3, and between group 2 and 3 of leukocyte number, but no significant for other groups. Meanwhile, the result of independent t-test showed no significant between all groups of the type of leukocyte. The experiment result showed that red pomegranate peel extract can significantly decrease the leukocyte number in rats exposed with mobile phone electromagnetic radiation (p<0.05), but no significant for different count (p>0.05).

Effects of pomegranate peel extract and vitamin E on the inflammatory status and endothelial function in hemodialysis patients: a randomized controlled clinical trial

2020 ◽  
Vol 11 (9) ◽  
pp. 7987-7993
Author(s):  
Tina Jafari ◽  
Aziz A. Fallah ◽  
Ali Reyhanian ◽  
Elham Sarmast
Keyword(s):  
Clinical Trial ◽  
Vascular Function ◽  
Hemodialysis Patients ◽  
Controlled Clinical Trial ◽  
Randomized Controlled Clinical Trial ◽  
Pomegranate Peel ◽  
Randomized Controlled ◽  
Inflammatory Status ◽  
Pomegranate Peel Extract ◽  
Peel Extract

This study showed that combination therapy with PPE and Vit E is more effective than single therapy to ameliorate inflammation and vascular function in hemodialysis patients.

scholarly journals Effects of Pomegranate Peel Extract and Vitamin E on Quality of Life in Hemodialysis Patients: A Randomized Placebo-Controlled Clinical Trial

2020 ◽  
Vol 7 (3) ◽  
pp. 129-135
Author(s):  
Tina Jafari ◽  
Leila Mahmoodnia ◽  
Mohsen Saeedi
Keyword(s):  
Quality Of Life ◽  
Clinical Trial ◽  
Placebo Group ◽  
Vitamin E ◽  
Short Form ◽  
Bodily Pain ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Background and aims: Quality of life (QOL) is poor in hemodialysis (HD) patients. High oxidative stress and inflammatory conditions disturb their normal physiological, emotional, and physical functions. This study aimed to assess the effects of pomegranate peel extract (PPE) alone and in combination with vitamin E (Vit E) as anti-oxidant and anti-inflammatory substances on QOL of HD patients using Short-form 36 (SF-36) QOL questionnaire. Methods: This study was a double-blinded, placebo-controlled randomized clinical trial on HD patients. A total of 100 HD patients were randomly divided into 4 equal groups as follows: Pom+Vit E group, which received 2 PPE tablets + 1 Vit E soft gel daily, Pom group, which received 2 PPE tablets+1 Vit E placebo soft gel daily, Vit E group, which received 1 Vit E soft gel+2 PPE placebo tablets daily, and Placebo group, which received 2 PPE placebo tablets + 1 Vit E placebo soft gel daily. The intervention duration was 8 weeks. The stratified block randomization method based on sex, age, HD duration, and employment status was used for randomization. Results: The mean age of participants ranged between 51 and 57 years with an HD duration of 9-11.2 months. Bodily pain score and general health score significantly increased in the Pom group and Pom+ Vit E group. The emotional role functioning score of the Pom+Vit E group was significantly higher than that of the placebo group (P<0.05). Conclusion: The consumption of PPE and Vit E had beneficial effects on mental components but not the physical components of QOL. Moreover, combination therapy was more effective than single therapy.

Effectiveness of Resin-Based Sealants with and without Fluoride Placed in a High Caries Risk Population: Multicentric 2-Year Randomized Clinical Trial

2018 ◽  
Vol 52 (4) ◽  
pp. 312-322 ◽  
Author(s):  
Michèle Muller-Bolla ◽  
Fréderic Courson ◽  
Laurence Lupi-Pégurier ◽  
Corinne Tardieu ◽  
Shrisha Mohit ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Retention Rate ◽  
Intervention Group ◽  
Assessment System ◽  
Caries Detection ◽  
Control Group ◽  
Caries Risk ◽  
High Caries Risk

A split-mouth randomized clinical trial was carried out to assess the effectiveness of sealants in preventing carious ICDAS (International Caries Detection and Assessment System) 3–6 lesions within a 2-year follow-up. We evaluated the effectiveness of 2 types of resin-based sealants, with and without fluoride, their retention rates, and the caries risk factors related to their outcomes. The study included 663 tooth pairs in 400 children (aged 5–15 years) considered to be at high individual caries risk (ICR) and presenting permanent molars free of caries or affected by ICDAS 1–2 lesions. In the first randomization, molars were either randomized to the treatment group receiving a dental sealant or the control group (nontreatment), and in the second randomization the sealant material to be used in the intervention group was selected. Uni- and multivariate Cox proportional hazard models were employed. At the 2-year follow-up, 483 tooth pairs were assessed: sealed molars had 83% (adjusted HR = 0.17; 95% CI: 0.15–0.20) less risk of developing ICDAS 3–6 lesions than molars without sealant. The magnitude of the protective effect was lower among teeth with ICDAS 1–2 lesions or with occlusal deep fissures than without. If the total retention rate of sealants was 70% at 2 years, sealant loss was not associated with the risk of caries (HR = 1.29, 95% CI: 0.92–1.79, p = 0.14). Sealants allow the prevention of new ICDAS 3–6 lesions or progression of noncavitated carious lesions in children at high ICR, and the effect of the sealant was similar regardless of whether it contained fluoride or not.

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