Autoantibodies against p53, MMP-7, and Hsp70 as Potential Biomarkers for Detection of Nonmelanoma Skin Cancers

Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are two predominant histological types of nonmelanoma skin cancer (NMSC), lacking effective early diagnostic markers. In this study, we assessed the diagnostic value of autoantibodies against p53, MMP-7, and Hsp70 in skin SCC and BCC. ELISA was performed to detect levels of autoantibodies in sera from 101 NMSC patients and 102 normal controls, who were recruited from the Cancer Hospital of Shantou University Medical College. A receiver operator characteristic curve was used to evaluate the diagnostic value. The serum levels of autoantibodies against p53, MMP-7, and Hsp70 were higher in NMSCs than those in the normal controls (all P < 0.01 ). The AUC of the three-autoantibody panel was 0.841 (95% CI: 0.788-0.894) with the sensitivity and specificity of 60.40% and 91.20% when differentiating NMSCs from normal controls. Furthermore, measurement of this panel could differentiate early-stage skin cancer patients from normal controls (AUC: 0.851; 95% CI: 0.793-0.908). Data from Oncomine showed that the level of p53 mRNA was elevated in BCC ( P < 0.05 ), and the Hsp70 mRNA was upregulated in SCC ( P < 0.001 ). This serum three-autoantibody panel might function in assisting the early diagnosis of NMSC.

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0588 Ring Pulse Oximeter for Screening of Moderate to Severe OSA: A Pilot Study

SLEEP ◽

10.1093/sleep/zsaa056.585 ◽

2020 ◽

Vol 43 (Supplement_1) ◽

pp. A225-A225

Author(s):

J Xue ◽

R Zhao ◽

J Li ◽

L Zhao ◽

B Zhou ◽

...

Keyword(s):

Pilot Study ◽

Pulse Oximeter ◽

Characteristic Curve ◽

Diagnostic Value ◽

Receiver Operator Characteristic Curve ◽

Oxygen Desaturation ◽

Significant Difference ◽

Acceptance Range ◽

The Difference ◽

Two Parameters

Abstract Introduction To evaluate the utility of the ring pulse oximeter for screening of OSA in adults. Methods 87 adults were monitored by a ring pulse oximeter and PSG simultaneously during a nocturnal in-lab sleep testing. 3% oxygen desaturation index (ODI3); Mean oxygen saturation(MSpO2), Saturation impair time below 90% (SIT90) derived from an automated algorithm of the ring pulse oximeter. Meanwhile, the parameters of PSG were scored manually according to the AASM Manual. Correlation and receiver operator characteristic curve analysis were used to measure the accuracy of ring pulse oximeter and its diagnostic value for moderate to severe OSA (AHI≥15). Results Among the 87 participants, 18 cases were AHI<5, 17 cases were diagnosed with mild OSA (AHI:5-14.9), 25 cases were diagnosed with moderate OSA (AHI:15-29.9) and 27 cases were diagnosed with severe OSA (AHI≥30). There was no significant difference between PSG and ring pulse oximeter in regard to ODI3 (23.4±23.5 vs 24.7 ± 21.7), and SIT90 (1.54%, range 0.14%-8.99% vs. 3.20%, range 0.60%, 12.30%) (P>0.05], Further analysis indicated that two parameters from the oximeter correlated well with that derived from PSG (r=0.889, 0.567, respectively, both p<0.05). Although MSpO2 correlated significantly (r=0.448, P<0.05), the difference was remarkable [95.9%, range 94.0% to 97.0% vs. 94.5%, range 93.3% to 95.7%, p<0.05]. Bland-Altman plots showed that the agreement of these three parameters was within the clinical acceptance range. The ROC curve showed that the sensitivity and specificity of the ring pulse oximeter when the oximeter derived ODI3 ≥12.5 in the diagnosis of moderate to severe OSA were 82.7% and 74.3%, respectively. Conclusion The pilot study indicated that ring pulse oximeter can detect oxygen desaturation events accurately, therefore to be used as a screening tool for moderate to severe OSA. Support The study was supported by the National Natural Science Foundation of China (No. 81420108002 and NO. 81570083).

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Squamous cell carcinoma antigen serum levels as prognostic parameter in patients with early stage vulvar cancer

Gynecologic Oncology ◽

10.1016/j.ygyno.2005.03.028 ◽

2005 ◽

Vol 97 (3) ◽

pp. 904-907 ◽

Cited By ~ 5

Author(s):

Lukas A. Hefler ◽

Gerhard Sliutz ◽

Sepp Leodolter ◽

Paul Speiser ◽

Elmar Joura ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Vulvar Cancer ◽

Early Stage ◽

Serum Levels ◽

Squamous Cell Carcinoma Antigen ◽

Prognostic Parameter

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Serum CYR61 As A Potential Biomarker for The Diagnosis of Esophagogastric Junction Tumor

Bioscience Reports ◽

10.1042/bsr20204117 ◽

2021 ◽

Author(s):

Ling-Yu Chu ◽

Jian-Yuan Zhou ◽

Yi-Xuan Zhao ◽

Yan-Ting Ou ◽

Tian Yang ◽

...

Keyword(s):

Early Stage ◽

Area Under The Curve ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Operating Characteristics ◽

Tumor Patients ◽

Chi Square ◽

Potential Biomarker ◽

The Difference ◽

Normal Controls

Background：Esophagogastric junction tumor (EGJ) is a rare but fatal disease with a rapid rising incidence worldwide in the late 20 years, and it lacks a convenient and safe method for diagnosis. This study aimed to evaluate the potential of serum CYR61 as a biomarker for the diagnosis of EGJ tumor. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to estimate CYR61 levels in sera of 152 EGJ tumor patients and 137 normal controls. Receiver operating characteristics (ROC) was carried out to evaluate the diagnostic accuracy. The Mann–Whitney’s U test was used to compare the difference of serum levels of CYR61 between groups. And chi-square tests were employed to estimate the correlation of the positive rate of serum CYR61 between/among subgroups. Results: Serum CYR61 levels were statistically lower in EGJ tumor and early-stage EGJ tumor patients than those in normal controls (P<0.0001). The sensitivity, specificity, and the area under the curve (AUC) of this biomarker in EGJ tumor were 88.2%, 43.8% and 0.691, respectively, and those for early stage of EGJ tumor were 80.0%, 66.4% and 0.722, respectively. Analyses showed that there was no correlation between the clinical data and the levels of CYR61 (P>0.05). Conclusion: This study showed that CYR61 might be a potential biomarker to assist the diagnosis of EGJ tumor.

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The Diagnostic Value of Mir-133a in ST Elevation and Non-ST Elevation Myocardial Infarction: A Meta-Analysis

Cells ◽

10.3390/cells9040793 ◽

2020 ◽

Vol 9 (4) ◽

pp. 793

Author(s):

Yehuda Wexler ◽

Udi Nussinovitch

Keyword(s):

Myocardial Infarction ◽

Cardiovascular Disease ◽

Diagnostic Performance ◽

Characteristic Curve ◽

Diagnostic Value ◽

Receiver Operator Characteristic Curve ◽

St Elevation Myocardial Infarction ◽

Diagnostic Potential ◽

St Elevation ◽

The Impact

Numerous studies have reported correlations between plasma microRNA signatures and cardiovascular disease. MicroRNA-133a (Mir-133a) has been researched extensively for its diagnostic value in acute myocardial infarction (AMI). While initial results seemed promising, more recent studies cast doubt on the diagnostic utility of Mir-133a, calling its clinical prospects into question. Here, the diagnostic potential of Mir-133a was analyzed using data from multiple papers. Medline, Embase, and Web of Science were systematically searched for publications containing “Cardiovascular Disease”, “MicroRNA”, “Mir-133a” and their synonyms. Diagnostic performance was assessed using area under the summary receiver operator characteristic curve (AUC), while examining the impact of age, sex, final diagnosis, and time. Of the 753 identified publications, 9 were included in the quantitative analysis. The pooled AUC for Mir-133a was 0.73. Analyses performed separately on studies using healthy vs. symptomatic controls yielded pooled AUCs of 0.89 and 0.68, respectively. Age and sex were not found to significantly affect diagnostic performance. Our findings indicate that control characteristics and methodological inconsistencies are likely the causes of incongruent reports, and that Mir-133a may have limited use in distinguishing symptomatic patients from those suffering AMI. Lastly, we hypothesized that Mir-133a may find a new use as a risk stratification biomarker in patients with specific subsets of non-ST elevation myocardial infarction (NSTEMI).

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The Diagnostic Value of Serum L1CAM in Patients With Colorectal Cancer

Technology in Cancer Research & Treatment ◽

10.1177/1533033820920971 ◽

2020 ◽

Vol 19 ◽

pp. 153303382092097

Author(s):

Ling-Yu Chu ◽

Dong-Ming Guo ◽

Jun-Tian Chen ◽

Wang-Kai Fang ◽

Jian-Jun Xie ◽

...

Keyword(s):

Colorectal Cancer ◽

Cell Adhesion ◽

Confidence Interval ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Operating Characteristic ◽

Early Stage ◽

Diagnostic Value ◽

L1 Cell Adhesion Molecule ◽

Normal Controls

Objective: Colorectal cancer is one of the most important malignant cancer in the world with high incidence and mortality. Some studies have found that the expression of low serum L1 cell adhesion molecule is associated with poor prognosis in some malignancies. It is suggested that L1 cell adhesion molecule is a candidate serum marker for certain tumors. However, the relationship between serum L1 cell adhesion molecule and colorectal cancer, especially about the diagnostic value, is rarely reported. Therefore, this study aimed to evaluate the diagnostic potential of serum L1 cell adhesion molecule in patients with colorectal cancer. Methods: Enzyme-linked immunosorbent assay was carried out to detect L1 cell adhesion molecule level in sera of 229 patients with colorectal cancer and 145 normal controls. Receiver operating characteristic curves were employed to calculate the accuracy of diagnosis. Results: The levels of serum L1 cell adhesion molecule in the colorectal cancer group were significantly lower than that in normal controls ( P < .05). In the normal group, the area under the receiver operating characteristic curve (area under the curve) of all colorectal cancer was 0.781 (95% confidence interval: 0.734-0.828) and early-stage colorectal cancer was 0.764 (95% confidence interval: 0.705-0.823). With optimized cutoff of 17.760 ng/mL, L1 cell adhesion molecule showed certain diagnostic value with specificity of 90.3% and sensitivities of 43.2% and 36.2% in colorectal cancer and early-stage colorectal cancer, respectively. Clinical data analysis showed that the levels of L1 cell adhesion molecule were significantly correlated with gender ( P < .05) and early and late stages ( P < .05). Furthermore, when compared with carcinoembryonic antigen, serum L1 cell adhesion molecule had significantly improved diagnostic accuracy for both colorectal cancer and early-stage colorectal cancer. Conclusions: Our study demonstrated that serum L1 cell adhesion molecule might be served as a potential biomarker for the diagnosis of colorectal cancer.

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The diagnostic value of serum levels of VEGF, bFGF and endostatin in patients with liver cancer

Journal of Clinical Oncology ◽

10.1200/jco.2007.25.18_suppl.21133 ◽

2007 ◽

Vol 25 (18_suppl) ◽

pp. 21133-21133

Author(s):

A. Nikopoulou ◽

P. Makrantonakis ◽

M. Karamouzis ◽

P. Nikolaidis ◽

J. Agorastos ◽

...

Keyword(s):

Growth Factor ◽

Serum Level ◽

Liver Cancer ◽

High Specificity ◽

Serum Levels ◽

Diagnostic Value ◽

Primary Hepatocellular Carcinoma ◽

Myocardial Infraction ◽

Group B ◽

Normal Controls

21133 Background: : Aim of this study is to evaluate the diagnostic value of serum levels of vascular endothelial growth factor ( VEGF ), basic fibroblast growth factor ( bFGF ) and Endostatin in patients with primary hepatocellular carcinoma ( PHC ) and in patients with metastatic liver disease ( MLD ). Methods: Eighty participants were included in this study and divided into three groups. Exclusion criteria were history of myocardial infraction, stroke, diabetic retinopathy, rheumatoid arthritis, psoriatic arthritis, pregnancy, trauma and recent surgical treatment. In group A were included 20 normal controls (NC), in group B 30 patients with PHC and in group C 30 patients with MLD. The concentrations of VEGF, bFGF and Endostatin in serum were measured by using enzyme like immunosorbent assay kits (Quantikine R&D systems Inc., Mineapolis.MN). Results: Results are shown in table . In patients with PHC there was a positive correlation between the serum level of VEGF and tumor size (r=0.517, p=0.08), between the serum level of VEGF and platelets number (r=0.573, p=0.003) and between the serum level of VEGF and the serum level of a-fetoprotein (r=0.478, p=0.029). In patients with PHC sensitivity of VEGF, bFGF and Endostatin was found 60%, 54% and 23% respectively. In patients with MLD sensitivity of VEGF, bFGF and Endostatin was found 73%, 73% and 27.5% respectively. However, both in patients with PHC and with MLD specificity of VEGF, bFGF and Endostatin were found 95%, 95% and 100% respectively. Conclusions: Angiogenic factors VEGF, bFGF and Endostatin can distinguish normal controls from patients with liver cancer. Serum levels of VEGF are related to the size of the tumor in patients with PHC. Serum VEGF, bFGF and Endostatin could be useful tumor markers in the diagnosis of PHC and MLD because of their high specificity. The significant correlation of VEGF with a- fetoprotein indicates its importance as a marker in diagnosis of PHC. [Table: see text] No significant financial relationships to disclose.

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Circulating lncRNA DLG1-AS1 in Plasma as a Novel Diagnostic Marker for Esophageal Squamous Cell Carcinoma (ESCC)

10.21203/rs.3.rs-43654/v1 ◽

2020 ◽

Author(s):

Xiuqin Wei ◽

Qiang Gao ◽

Wei Jia ◽

Chao Ma ◽

Mei Xue ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Operating Characteristic ◽

Early Stage ◽

Diagnostic Value ◽

Healthy Controls ◽

Healthy Control

Abstract BACKGROUND: Esophageal squamous cell carcinoma (ESCC) in some cases can be diagnosed as esophageal varices (EV). DLG1-AS1 promotes cervical cancer, while its function is other malignancies remains unknown. Our aim for this study is to study the role of DLG1-AS1 in esophageal squamous cell carcinoma.METHODS: Plasma levels of DLG1-AS1 in 66 early stage ESCC patients, 60 EV patients and 60 healthy controls were measured by RT-qPCR. Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of DLG1-AS1 for early stage ESCC. Relationship between miR-145 and DLG1-AS1 was analyzed by overexpression experiments. Proliferation of cells was determined by CCK-8 assay. RESULTS: DLG1-AS1 was upregulated in ESCC, but not in EV patients compared with healthy control. DLG1-AS1 overexpression distinguished ESCC patients from healthy controls and EV patients. Plasma miR-145 was inversely correlated with DLG1-AS1 in ESCC patients. Moreover, DLG1-AS1 overexpression resulted in the downregulation of miR-145, while miR-145 mimic transfection did not significantly alter DLG1-AS1. Overexpression of DLG1-AS1 mediated the promoted, while overexpression of miR-145 resulted in inhibited proliferation of ESCC cells. The role of DLG1-AS1 overexpression was inhibited by miR-145 mimic transfection. CONCLUSION: Therefore, DLG1-AS1 may promote ESCC under the repression of miR-145.

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Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease

Frontiers in Neurology ◽

10.3389/fneur.2021.680765 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xu Yi ◽

Yujia Yang ◽

Zhengfan Zhao ◽

Manyu Xu ◽

Yuan Zhang ◽

...

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Early Stage ◽

High Sensitivity ◽

Serum Levels ◽

Diagnostic Value ◽

Diagnostic Techniques ◽

Mature Brain ◽

Early Diagnostic

Parkinson's disease (PD) is one of the most common chronic, progressive, and neurodegenerative diseases characterized clinically by resting tremor, bradykinesia, rigidity, and postural instability. As this disease is usually detected in the later stages, the cure is often delayed, ultimately leading to disability due to the lack of early diagnostic techniques. Therefore, it is of great importance to identify reliable biomarkers with high sensitivity and specificity for the early diagnosis of PD. In this study, we aimed to investigate whether serum expressions of mature brain-derived neurotrophic factor (mBDNF) and proBDNF can serve as biomarkers for the diagnosis of PD at early stage. One hundred and fifty-six patients with limb tremor and/or bradykinesia meeting the inclusion criteria were assigned to either ex-PD group (PD cases) or ex-NPD group (non-PD cases) and then reassigned to either po-PD group (with PD) or po-NPD group (without PD) at 1-year follow-up based on the results of the rediagnoses as performed in accordance with MDS Parkinson's diagnostic criteria. To improve early diagnostic accuracy, grouping (PD group and non-PD group) at initial visit and follow-up was performed differently and independently. Serum mBDNF and proBDNF levels were measured by enzyme-linked immunosorbent assays. The results demonstrated that serum levels of mBDNF and mBDNF/proBDNF were significantly lower in the ex-PD group (19.73 ± 7.31 and 0.09 ± 0.05 ng/ml) as compared with the ex-NPD group (23.47 ± 8.21 and 0.15 ± 0.12 ng/ml) (p < 0.01 for both) and in the po-PD group (19.24 ± 7.20 and 0.09 ± 0.05 ng/ml) as compared with the po-NPD group (25.05 ± 7.67 and 0.16 ± 0.14 ng/ml) (p < 0.01 for both). However, a significantly higher serum level of proBDNF was noted in the ex-PD group (235.49 ± 60.75 ng/ml) as compared with the ex-NPD group (191.75 ± 66.12 ng/ml) (p < 0.01) and in the po-PD group (235.56 ± 60.80 ng/ml) as compared with the po-NPD group (188.42 ± 65.08 ng/ml) (p < 0.01). In conclusion, mBDNF/proBDNF can be used as biomarkers for early stage Parkinson's disease; in addition, mBDNF plus proBDNF has better diagnostic value than mBDNF alone in the diagnosis of PD.

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Surfactant Protein D and KL-6 as Serum Biomarkers of Interstitial Lung Disease in Patients with Scleroderma

The Journal of Rheumatology ◽

10.3899/jrheum.080633 ◽

2009 ◽

Vol 36 (4) ◽

pp. 773-780 ◽

Cited By ~ 103

Author(s):

FAYE N. HANT ◽

ANNA LUDWICKA-BRADLEY ◽

HE-JING WANG ◽

NING LI ◽

ROBERT ELASHOFF ◽

...

Keyword(s):

Interstitial Lung Disease ◽

Lung Disease ◽

Characteristic Curve ◽

High Sensitivity ◽

Serum Levels ◽

Surfactant Protein ◽

Receiver Operator Characteristic Curve ◽

Surfactant Protein D ◽

High Resolution Computed Tomography ◽

Type Ii Pneumocytes

Objective.To assess whether serum concentrations of surfactant protein D (SP-D) and Krebs von den Lungen-6 (KL-6), glycoproteins expressed by type II pneumocytes, correlate with the presence of “alveolitis” and measures of lung function in patients enrolled in the Scleroderma Lung Study (SLS).Methods.Serum obtained at baseline screening of patients with systemic sclerosis (SSc, scleroderma) in the SLS was assayed. “Alveolitis” was defined by either bronchoalveolar lavage or thoracic high-resolution computed tomography (HRCT) by SLS criteria. SP-D and KL-6 levels were measured by ELISA in 66 SSc patients (44 with “alveolitis,” 22 without “alveolitis”) and in 10 healthy controls. These were compared to clinical measures of lung disease and “alveolitis” in the SLS patients.Results.SP-D levels were 300 ± 214 ng/ml (mean ± SD) in the SSc patients compared to 40 ± 51 ng/ml in controls (p < 0.0001). KL-6 levels were 1225 ± 984 U/ml in the SSc patients and 333 ± 294 U/ml in controls (p < 0.0001). SSc patients with “alveolitis” had higher levels of both SP-D and KL-6 than those without “alveolitis.” The level of SP-D was 353 ± 219 ng/ml in patients with “alveolitis” and 161 ± 143 ng/ml without “alveolitis” (p = 0.0002). The level of KL-6 was 1458 ± 1070 U/ml in patients with “alveolitis” and 640 ± 487 U/ml without “alveolitis” (p = 0.0001). Receiver operator characteristic curve analysis demonstrated high sensitivity and specificity of both SP-D and KL-6 for the determination of “alveolitis.” KL-6 and SP-D were positively correlated with maximum fibrosis scores, but not with maximum ground-glass opacities, on HRCT.Conclusion.Serum levels of SP-D and KL-6 appear to be indicative of “alveolitis” in SSc patients as defined by the SLS, and are significantly higher than in SSc patients without “alveolitis.” Serum SP-D and KL-6 may serve as noninvasive serological means of assessing interstitial lung disease in patients with SSc.

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Development of Human Toxo IgG ELISA Kit, and False-Positivity of Latex Agglutination Test for the Diagnosis of Toxoplasmosis

Pathogens ◽

10.3390/pathogens10091111 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1111

Author(s):

Sarfraz-ur-Rahman Sarfraz-ur-Rahman ◽

Haroon Akbar ◽

Muhammad Zubair Shabbir ◽

Ubaid Ullah ◽

Muhammad Imran Rashid

Keyword(s):

Toxoplasma Gondii ◽

Characteristic Curve ◽

Diagnostic Value ◽

Latex Agglutination ◽

Receiver Operator Characteristic Curve ◽

Elisa Kit ◽

Metal Affinity ◽

Wide Range ◽

Zoonotic Parasite ◽

Human Sera

Toxoplasma gondii is an intracellular zoonotic parasite that causes infection in a wide range of warm-blooded animals and humans. The main aim of this studywas to assess the diagnostic value of the recombinant SAG1 antigen (rSAG1) for T. gondii-IgG screening through the Human Toxo IgG ELISA Kit (K). The rSAG1 was expressed in E. coli (DE3), and it was purified through metal-affinity chromatography. The rSAG1 was confirmed by immunoblotting, and it had a band on 35 kDa.Total of 400 human sera were tested by LAT and K. One hundred and twenty-two (30.5%) sera were found positive by LAT and eighty-nine (22.25%) sera were found positive by K.Out of 400 samples, 80 were selected to evaluate the performance of K through commercial Toxoplasma gondii IgG ELISA Kit (C). Out of 80 human sera, 55 (68.75%) were found positive, 25 (31.25%) were found negative by K and C, respectively. The cut-off value for K was 0.398 and it was calculated through the receiver operator characteristic curve. The ELISA plates were coated at optimized concentration of rSAG1 = 0.125 µg/mL, and the test was performed by diluting the sera at 1:50. The sensitivity and specificity of K were observed to be 98.5% and 100%, respectively. The six sera (K-L+were found positive through LAT and these human sera were later evaluated by Western blot analysis. These sera did not produce a band equivalent to 35 kDa on WB analysis thus, LAT produced false-positive results

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