Antibacterial Properties of Oregano Essential Oil Encapsulated in Poly(ε-Caprolactone) Nanoparticles

This work describes an outstanding synthesis of poly(ε-caprolactone) nanoparticles of distinct sizes loaded (PCLNP/OEO) or not (PCLNP) with oregano (Origanum vulgare) essential oil (OEO) by the nanoprecipitation method in order investigate the antimicrobial properties against bacteria Escherichia coli and Staphylococcus aureus. OEO antimicrobial activity was achieved by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). FTIR spectra indicated that encapsulation of OEO by PCLNPs was successful. Scanning electron microscopy images revealed that PCLNP and PCLNP/OEO presented spherical shape with diameters around 150 nm, while PCLNP exhibited size distribution raging 158 to 300 nm and zeta potential ranging from -13.8 to -28.5 mV. Thermogravimetric analysis indicated an increase in initial temperature of weight loss for PCLNP/OEO. PCLNP with 80% wt. of OEO has exhibited the highest decrease of growth of bacteria, and MIC against S. aureus and E. coli and MBC only for E. coli, which indicate a promising alternative for designing stable antimicrobial nanoparticles for application in novel food active packaging.

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Essential Oil and Ethanol Extract of Oregano (Origanum vulgare L.) from Armenian Flora as a Natural Source of Terpenes, Flavonoids and other Phytochemicals with Antiradical, Antioxidant, Metal Chelating, Tyrosinase Inhibitory and Antibacterial Activity

Current Pharmaceutical Design ◽

10.2174/1381612825666190702095612 ◽

2019 ◽

Vol 25 (16) ◽

pp. 1809-1816 ◽

Cited By ~ 9

Author(s):

Armenuhi Moghrovyan ◽

Naira Sahakyan ◽

Anush Babayan ◽

Naira Chichoyan ◽

Margarit Petrosyan ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Natural Source ◽

Origanum Vulgare ◽

Oregano Essential Oil ◽

E Coli ◽

Metal Chelating

Background: Origanum vulgare is used in Armenian cuisine as well as in folk medicine. The aim of this investigation was to determine the peculiarities of the chemical composition of Armenian flora’s oregano essential oil (EO) and ethanol extract (EE) and to characterize mainly antioxidant and antibacterial properties in vitro. Methods: EO of plant aerial parts was extracted by the hydro-distillation method, and the composition was analyzed by gas-chromatography (GC). EE was prepared using ethanol as a solvent. Antioxidant, antibacterial and other biological activity of EO and EE was determined using appropriate methods. Results: The terpenes were found to be the greatest part of oregano EO (β-caryophyllene epoxide - 13.3 %; β- caryophyllene -8.2 %; ο-cymene - 5.2 %). The flavonoid content of EE was 3.9±0.7 mg g-1 catechin equivalents. Antiradical activities of EO and EE expressed with half-maximum inhibitory concentrations were 1057 µg mL-1 and 19.97 µg mL-1. The antioxidant index in the case of using extract (1000 µg mL-1) was 77.3±1.5 %. The metal chelating activity of EE was 74.5±0.2 %. The tyrosinase inhibitory activities of EO, EE and arbutin were 26.5±0.3 %, 6.5±0.2 % and 50±0.1 %, respectively. Both EO and EE of oregano were able to suppress the growth of testmicroorganisms including clinical pathogen isolates, but have moderate antimicrobial activity. EO antibacterial activity against ampicillin-resistant Escherichia coli was similar to the activity against non-resistant strain. EE was not active against ampicillin-resistant E. coli in contrast to non-resistant one. Conclusion: The results indicate high antioxidant and moderate antibacterial as well as high antiradical, metal chelating, tyrosinase inhibitory activity of oregano EO and EE, which can be used as a natural source of terpenes, flavonoids and other phytochemicals in medicine, pharmaceutics, cosmetics and food industry. EO has antibacterial activity also against ampicillin-resistant E. coli.

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Characterization of Oregano Essential Oil (Origanum vulgare L. subsp. hirtum) Particles Produced by the Novel Nano Spray Drying Technique

Foods ◽

10.3390/foods10122923 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2923

Author(s):

Fotini Plati ◽

Rigini Papi ◽

Adamantini Paraskevopoulou

Keyword(s):

Essential Oil ◽

Spray Drying ◽

Encapsulation Efficiency ◽

Biological Activities ◽

Antibacterial Properties ◽

Spherical Shape ◽

Partial Replacement ◽

Structural Stabilization ◽

Oregano Essential Oil ◽

Wall Materials

Oregano essential oil (OEO), due to its wide variety of biological activities, could be a “green” alternative to chemical preservatives. On the other hand, the difficulties in its use or storage have turned researchers’ interest in encapsulation strategies as a way to face stability and handling issues. Fabrication of OEO-loaded particles, using nano spray drying technique (NSD) and whey protein isolate-maltodextrin mixtures (1:1, 1:3) as wall materials appears to be a novel and promising strategy. The obtained particles were characterized in terms of volatile composition, encapsulation efficiency, and physicochemical, molecular, morphological, and antibacterial properties. The results confirmed that encapsulation of OEO using NSD achieved high levels of powder recovery (>77%) and encapsulation efficiency (>98%) while assisting in the retention of the main bioactive compounds. The partial replacement of WPI by MD significantly affected particles’ physical properties. FTIR analyses revealed the possible structural stabilization of core and wall materials, while SEM verified the very fine size and spherical shape. Finally, antibacterial studies demonstrated their activity against Escherichia coli and Staphylococcus aureus, which is much stronger in comparison with that of pure OEO, proving the positive effect of NSD and particles’ potential in future food applications.

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Antioxidant and Antimicrobial Properties of Chitosan-PE Bi-Layer Films by Incorporating Oregano Essential Oil

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.469.140 ◽

2013 ◽

Vol 469 ◽

pp. 140-147 ◽

Cited By ~ 3

Author(s):

Hui Juan Bao ◽

Yan Jiang ◽

Jia Qi Liu ◽

Hai Yue Zhang

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Antimicrobial Properties ◽

Reducing Power ◽

Chitosan Solution ◽

Dpph Radical ◽

Oregano Essential Oil ◽

E Coli ◽

Scavenging Effect

Chitosan-PE bi-layer films exhibit antimicrobial activity but poor antioxidant activity. Oregano essential oil (100μL/100mL) was incorporated into chitosan solution to improve films antioxidant and antimicrobial behaviors. The present work investigated the scavenging of O2·, ·OH, DPPH radical and reducing power of film, antimicrobial activity was also evaluated. The results showed that the scavenging effect of film solution on O2·, ·OH, and DPPH was 80.87%, 85.56%, and 87.24%, respectively. Reducing power of film increased with the improvement of oregano essential oil. The antibacterial activity againstE. ColiandB. Subtilisincreased from 80.77% to 96.15% and from 93.02% to 98.10%, respectively. The preservation experiments showed that chitosan-PE film incorporated with oregano essential oil could extend the shelf life of pork to 810 days at 4°C.

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Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-Resistant Escherichia coli

Microorganisms ◽

10.3390/microorganisms10010109 ◽

2022 ◽

Vol 10 (1) ◽

pp. 109

Author(s):

Mohammadreza Pajohi Alamoti ◽

Behnaz Bazargani-Gilani ◽

Razzagh Mahmoudi ◽

Anna Reale ◽

Babak Pakbin ◽

...

Keyword(s):

Escherichia Coli ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Properties ◽

Multidrug Resistant ◽

Zataria Multiflora ◽

Cuminum Cyminum ◽

Plant Essential Oils ◽

E Coli ◽

Mentha Longifolia

Aim of this study was to investigate the antimicrobial properties of herbal plant essential oils (EOs) from selected Iranian plant species such as Ferulago angulata, Zataria multiflora, Cuminum cyminum, and Mentha longifolia against antibiotic-resistant Escherichia coli (E. coli) strains. For this purpose, the Escherichia coli strains, isolated from raw cow’s milk and local dairy products (yogurt, cream, whey, cheese, and confectionery products) collected from different areas of Hamedan province, Iran, were investigated for their resistance to antibiotics (i.e., streptomycin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin, and cefixime). Thus, the E. coli strains were tested for their susceptibility to the above-mentioned essential oils. Regarding antibiotics, the E. coli strains were highly sensitive to ciprofloxacin. In relation to essential oils, the most effective antibacterial activity was observed with Zataria multiflora; also, the bacteria were semi-sensitive to Cuminum cyminum and Mentha longifolia essential oils. All strains were resistant to Ferulago angulata essential oil. According to the results, the essential oil of Zataria multiflora can be considered as a practical and alternative antibacterial strategy to inhibit the growth of multidrug-resistant E. coli of dairy origin.

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Production and Characterization of Keratin/Tragacanth Gum Nanohydrogels for Drug Delivery in Medical Textiles

Frontiers in Materials ◽

10.3389/fmats.2021.720385 ◽

2021 ◽

Vol 8 ◽

Author(s):

Nazanin Mansouri Shirazi ◽

Niloofar Eslahi ◽

Adeleh Gholipour-Kanani

Keyword(s):

Disulfide Bonds ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Spherical Shape ◽

Herbal Extract ◽

Minimum Size ◽

Mechanical Resistance ◽

Tragacanth Gum ◽

Medical Textiles

Keratin protein has been applied for biomedical applications due to its biocompatibility, biodegradability, mechanical resistance, and bioavailability. Tragacanth gum (TG) as a polysaccharide-based biopolymer has wound healing and antimicrobial properties. In this study, keratin was extracted from protein-based chicken feather by using reduction hydrolysis (sodium sulfide), and nanogels of keratin and TG composites at different ratios were produced by using the chemical cross-linking method. Then, cinnamon (5 and 10%) as an antibacterial herbal extract was added to the nanogels and coated on cotton fabric. The morphology and size of the composite nanogels, chemical structure, biological, and antibacterial properties were evaluated. According to DLS results, TGK2:1 (ratio of TG to keratin = 2:1) had the minimum size (80 nm) and PDI (0.1), and therefore, this sample was chosen as the optimum one. FESEM and TEM images showed the semi-spherical shape of the produced nanogels. FTIR spectra revealed the possible hydrogen bonding between the components, and the formation of disulfide bonds after the addition of hydrogen peroxide was confirmed by XPS. After loading cinnamon into the nanogels, an increase in size was observed from 80 nm for free-nanogel to 85 and 105 nm for 5 and 10% extract-loaded nanogels, respectively. Besides, more cinnamon was released from the treated fabrics by increasing time and cinnamon concentration. The antibacterial test exhibited good antibacterial properties against both Gram-positive and Gram-negative bacteria. Finally, MTT assay approved the biocompatibility of the produced nanogels for potential use in medical textiles.

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Improving the Quality of Turkey Meat via Storage Temperature, Packaging Atmosphere, and Oregano (Origanum vulgare) Essential Oil Addition

Agriculture ◽

10.3390/agriculture10100463 ◽

2020 ◽

Vol 10 (10) ◽

pp. 463

Author(s):

Samir A. Mahgoub ◽

Mohamed E. Abd El-Hack ◽

Zohair S. Mulla ◽

Waleed R. El-Ghareeb ◽

Ayman E. Taha ◽

...

Keyword(s):

Essential Oil ◽

Storage Temperature ◽

Modified Atmosphere Packaging ◽

Modern World ◽

Origanum Vulgare ◽

Oregano Essential Oil ◽

E Coli ◽

Turkey Meat ◽

Storage Temperatures

The provision of plentiful good-quality food is a primary issue in the modern world. This work was planned to study the influence of packaging atmosphere and oregano (Origanum vulgare) essential oil addition [(vacuum packaging: T1 or modified atmosphere packaging or T2 (CO2/N2 = 4:6) or T3, T2 with oregano essential oil (T2 + EO)] under various storage temperatures (0, 5, 10, and 15 °C) on the control of survival of Escherichia coli O157:H7 and associated spoilage flora in sliced smoked turkey meat. The pathogen increased by only <1.0 log colony-forming unit (CFU)/g under all packaging and temperature combinations. Moreover, T1, T2, and T3 exerted practically similar inhibitory activity against the pathogen and dominating bacteria, with a relatively low growth of E. coli O157:H7 in sliced smoked turkey during the shelf life under all storage regimes compared to the control. However, the pathogen survival was highest on the sliced smoked turkey under T1, decreasing by only 0.67, 0.74, 0.63, and 1.30 log CFU/g within 37 days if kept at 0, 5, 10, and 15 °C, respectively. Under T2 and the same condition, E. coli O157:H7 in the product declined by only 0.31, 0.50, 0.72, and 1.10 log CFU/g within 37 days of storage, respectively. In the T3 samples, the pathogen was reduced by only 0.33, 0.67, 1.72, and 3.46 log CFU/g through 37 days of storage, respectively. Under T3 were E. coli O157:H7 populations in smoked turkey eliminated (negative by enrichment) under all conditions (after 129, 95, 95, and 43 days maintained at 0, 5, 10, and 15 °C, respectively) compared with other packaging temperature combinations. Thus, T3 contributed to developing ready-to-eat smoked turkey with enhanced product quality and eliminating the pathogen.

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Synthesis and Antimicrobial Properties of Zinc Oxide Nanoparticles

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2020.18707 ◽

2020 ◽

Vol 20 (10) ◽

pp. 5977-5996 ◽

Cited By ~ 1

Author(s):

Saee Gharpure ◽

Balaprasad Ankamwar

Keyword(s):

Zinc Oxide ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Metal Oxide Nanoparticles ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Physical Contact ◽

Oxide Nanoparticles ◽

Synthesis Methods ◽

Promising Alternative

With increase in incidence of multidrug resistant pathogens, there is a demand to adapt newer approaches in order to combat these diseases as traditional therapy is insufficient for their treatment. Use of nanotechnology provides a promising alternative as antimicrobial agents as against traditional antibiotics. Metal oxides have been exploited for a long times for their antimicrobial properties. Zinc oxide nanoparticles (ZnO NPs) are preferred over other metal oxide nanoparticles because of their bio-compatible nature and excellent antibacterial potentials. The basic mechanism of bactericidal nature of ZnO nanoparticles includes physical contact between ZnO nanoparticles and the bacterial cell wall, generation of reactive oxygen species (ROS) as well as free radicals and release of Zn2+ ions. This review focuses on different synthesis methods of ZnO nanoparticles, various analytical techniques frequently used for testing antibacterial properties, mechanism explaining antibacterial nature of ZnO nanoparticles as well as different factors affecting the antibacterial properties.

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Electrospun Active Biopapers of Food Waste Derived Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Short-Term and Long-Term Antimicrobial Performance

Nanomaterials ◽

10.3390/nano10030506 ◽

2020 ◽

Vol 10 (3) ◽

pp. 506 ◽

Cited By ~ 5

Author(s):

Kelly J. Figueroa-Lopez ◽

Sergio Torres-Giner ◽

Daniela Enescu ◽

Luis Cabedo ◽

Miguel A. Cerqueira ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Properties ◽

High Antimicrobial Activity ◽

Short Term ◽

Oregano Essential Oil ◽

E Coli ◽

Closed Systems ◽

Antimicrobial Performance ◽

And Migration

This research reports about the development by electrospinning of fiber-based films made of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) derived from fermented fruit waste, so-called bio-papers, with enhanced antimicrobial performance. To this end, different combinations of oregano essential oil (OEO) and zinc oxide nanoparticles (ZnONPs) were added to PHBV solutions and electrospun into mats that were, thereafter, converted into homogeneous and continuous films of ~130 μm. The morphology, optical, thermal, mechanical properties, crystallinity, and migration into food simulants of the resultant PHBV-based bio-papers were evaluated and their antimicrobial properties were assessed against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in both open and closed systems. It was observed that the antimicrobial activity decreased after 15 days due to the release of the volatile compounds, whereas the bio-papers filled with ZnONPs showed high antimicrobial activity for up to 48 days. The electrospun PHBV biopapers containing 2.5 wt% OEO + 2.25 wt% ZnONPs successfully provided the most optimal activity for short and long periods against both bacteria.

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