Using Deep Learning Artificial Intelligence Algorithms to Verify N-Nitroso-N-Methylurea and Urethane Positive Control Proliferative Changes in Tg-RasH2 Mouse Carcinogenicity Studies

2020 ◽  
pp. 019262332097398
Author(s):  
Daniel Rudmann ◽  
Jay Albretsen ◽  
Colin Doolan ◽  
Mark Gregson ◽  
Beth Dray ◽  
...  
Keyword(s):  
Artificial Intelligence ◽  
Deep Learning ◽  
Decision Support ◽  
Positive Control ◽  
Control Group ◽  
Control Groups ◽  
Test Study ◽  
Provide Decision Support ◽  
Proliferative Lesion ◽  
High Concordance

In Tg-rasH2 carcinogenicity mouse models, a positive control group is treated with a carcinogen such as urethane or N-nitroso-N-methylurea to test study validity based on the presence of the expected proliferative lesions in the transgenic mice. We hypothesized that artificial intelligence–based deep learning (DL) could provide decision support for the toxicologic pathologist by screening for the proliferative changes, verifying the expected pattern for the positive control groups. Whole slide images (WSIs) of the lungs, thymus, and stomach from positive control groups were used for supervised training of a convolutional neural network (CNN). A single pathologist annotated WSIs of normal and abnormal tissue regions for training the CNN-based supervised classifier using INHAND criteria. The algorithm was evaluated using a subset of tissue regions that were not used for training and then additional tissues were evaluated blindly by 2 independent pathologists. A binary output (proliferative classes present or not) from the pathologists was compared to that of the CNN classifier. The CNN model grouped proliferative lesion positive and negative animals at high concordance with the pathologists. This process simulated a workflow for review of these studies, whereby a DL algorithm could provide decision support for the pathologists in a nonclinical study.

scholarly journals Assessing autonomous ship navigation using bridge simulators enhanced by cycle-consistent adversarial networks

2021 ◽  
pp. 1748006X2110210
Author(s):  
Andreas Brandsæter ◽  
Ottar L Osen
Keyword(s):  
Artificial Intelligence ◽  
Deep Learning ◽  
Decision Support ◽  
Object Detection ◽  
Autonomous Navigation ◽  
Adversarial Networks ◽  
Maritime Navigation ◽  
Ship Navigation ◽  
Autonomous Ship ◽  
Ship Operation

The advent of artificial intelligence and deep learning has provided sophisticated functionality for sensor fusion and object detection and classification which have accelerated the development of highly automated and autonomous ships as well as decision support systems for maritime navigation. It is, however, challenging to assess how the implementation of these systems affects the safety of ship operation. We propose to utilize marine training simulators to conduct controlled, repeated experiments allowing us to compare and assess how functionality for autonomous navigation and decision support affects navigation performance and safety. However, although marine training simulators are realistic to human navigators, it cannot be assumed that the simulators are sufficiently realistic for testing the object detection and classification functionality, and hence this functionality cannot be directly implemented in the simulators. We propose to overcome this challenge by utilizing Cycle-Consistent Adversarial Networks (Cycle-GANs) to transform the simulator data before object detection and classification is performed. Once object detection and classification are completed, the result is transferred back to the simulator environment. Based on this result, decision support functionality with realistic accuracy and robustness can be presented and autonomous ships can make decisions and navigate in the simulator environment.

scholarly journals Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

2020 ◽  
Vol 18 (1) ◽  
pp. 47
Author(s):  
FERIZAL NEGERI SAMUDRA ◽  
RETNO BUDIARTI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Sea Cucumber ◽  
Antibacterial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Control Groups ◽  
Shigella Spp ◽  
Diarrhea Disease

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on <em>Shigella Dysentriae</em> growth in vitro is needed.</p><p><strong>Objectives</strong>: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the <em>Shigella dysentriae</em> bacteria in vitro.</p><p><strong>Method</strong>: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.</p><p><strong>Result</strong>: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.</p><p><strong>Conclusion</strong>: Sea cucumber (<em>Holoturia scabra</em>) inhibit the growth of <em>Shigella dysenteriae</em>.</p><p><strong>Key words</strong>: <em>Shigella dysenteriae</em>, sea cucumber (<em>Holoturia scabra</em>), antibacterial</p>

scholarly journals RUELLIA TUBEROSA LINN. ACTS AS ANTI-FERTILITY AGENT THAT REDUCES SPERM COUNT, MOTILITY AND VIABILITY IN MALE SWISS ALBINO MICE (MUS-MUSCULUS)

2016 ◽  
Vol 9 (1) ◽  
pp. 105
Author(s):  
Pardeshi M. H. ◽  
Deshmukh A. A. ◽  
Gajare K. A.
Keyword(s):  
Mus Musculus ◽  
Sperm Count ◽  
Positive Control ◽  
Male Reproduction ◽  
Control Group ◽  
Global Public Health ◽  
Control Groups ◽  
Swiss Albino Mice ◽  
Sperm Suspension ◽  
Albino Mice

Objective: Fertility control is an issue of global public health. Many of the contraceptives available today have one or the other side effects. Many plants and plant products are suggested as contraceptives in folk and traditional systems of medicine. However, that are least exploited in this regard. In the present investigation, root powder of Ruellia tuberosa was studied for its effect on male reproduction in mice.Methods: The Swiss albino mice, Mus musculus of age three months were grouped into four, i)control group, fed on standard pellet, ii)experimental groups I and II received root powder of Ruellia tuberosa 50 mg/mouse/days for 15 d and 30 d respectively in the pellets, iii)positive control groups I and II received cotton seed oil 25 µl/mouse/day for 15 and 30 d and iv)recovery group received Ruellia tuberosa (50 mg/mouse/days) containing pellets for 15 d and later standard pellet for 15 d. Cauda epididymis sperm suspension was analyzed for sperm count, motility and viability.Results: There was a highly significant decrease in sperm count, motility and viability (p<0.001) in experimental groups I and II and positive control groups I and II. The sperm count was reduced to 19.24±1.74 million/ml and 15.97±5.61 million/ml as compared to sperm count in control group (55.12±4.63 million/ml) in experimental groups. Partial reversal of the effect was noticed in a recovery group.Conclusion: The results suggest that Ruellia tuberosa can be a potent member of reversible oral male contraceptives.

scholarly journals Efficacy of a herbal toothpaste on patients with established gingivitis: a randomized controlled trial

2006 ◽  
Vol 20 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Fabiana Ozaki ◽  
Claudio Mendes Pannuti ◽  
Ana Vitória Imbronito ◽  
Wellington Pessotti ◽  
Luciana Saraiva ◽  
...  
Keyword(s):  
Adverse Reactions ◽  
Controlled Trial ◽  
Test Group ◽  
Experimental Period ◽  
Positive Control ◽  
Control Group ◽  
Control Groups ◽  
Double Blind ◽  
Significant Difference ◽  
And Control

The aim of this randomised, double blind controlled trial was to verify the efficacy of a herbal dentifrice on the reduction of plaque and gingivitis. Forty eight volunteers with established gingivitis were randomly assigned to either a test group (herbal dentifrice) or positive control group (dentifrice with triclosan and fluoride). The dentifrices were distributed in plain white tubes by an independent pharmacy, which revealed the contents of each tube only after the experimental period. Plaque and gingivitis assessments were carried out on baseline and after 28 days of product use. All examinations were conducted by the same calibrated investigator. Subjects were instructed to brush their teeth three times daily using their assigned dentifrice for 28 days. There was a significant reduction in plaque levels in both the test and control groups. However, there was no significant difference between the groups. A significant reduction in gingivitis was observed in both groups, although there was no significant difference between them. No adverse reactions were reported. The authors concluded that both dentifrices were effective in reducing plaque and gingivitis in subjects with established gingivitis.

scholarly journals Estrogenic effects of silymarin in ovariectomized rats 

2001 ◽  
Vol 46 (No. 1) ◽  
pp. 17-23 ◽  
Author(s):  
V. Kummer ◽  
J. Mašková ◽  
J. Čanderle ◽  
Z. Zralý ◽  
J. Neča ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Positive Control ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Minor Role ◽  
Control Groups ◽  
Estrogenic Effects ◽  
Ovx Rats ◽  
A Minor ◽  
Negative Controls

The objective of this study was to test whether silymarin induces changes indicative of estrogenic effects in gonadal organs of ovariectomized (OVX) rats. Silymarin was administered in two experimental groups of OVX rats (n&nbsp;= 7 + 7) for 30 days at the doses of 25 or 50 mg per animal per day. OVX rats (n&nbsp;= 7) receiving 5&nbsp;&micro;g of 17b-estradiol (E2) for the last three days before killing and untreated OVX rats (n&nbsp;= 7) were used as the positive and the negative controls, respectively. Uterine and blood samples were collected immediately after killing. Compared with the negative controls, total and normalized uterine weights were significantly higher in the two experimental groups (P&nbsp;&lt; 0.01 and&nbsp;P&nbsp;&lt;&nbsp;0.05, respectively). Uterotrophic effects of silymarin were also evident from increased heights of the luminal epithelium (P&nbsp;&lt; 0.01) and the endometrium (P&nbsp;&lt; 0.05). The response was dose-independent within the tested range. The strongest uterine response was observed in the OVX rats treated with E2. A highly significant decrease in mean density of estrogen receptor (ERa) immunostaining in the luminal and the glandular endometrial epithelia (P&nbsp;&lt; 0.01) and a stronger ERa immunostaining in stromal cells were observed in the two experimental and the positive control groups. The activities of alkaline and acid phosphatases were significantly increased in the luminal (P&nbsp;&lt; 0.05) and the glandular (P&nbsp;&lt; 0.01) epithelia only in the rats treated with E2. Both silymarin and E2&nbsp;induced an increase in thyroid hormone concentrations in blood serum. The rises of free T3&nbsp;and T4&nbsp;were significant (P&nbsp;&lt; 0.05) in the group receiving 50 mg of silymarin per day. Hepatic oxidative metabolism of steroids was assumed to be another target of the action of silymarin. The mitochondrial cytochrome&nbsp;P450-dependent testosterone hydroxylase activity and the cytochrome&nbsp;P4501A-dependent 7-ethoxyresorufin O-deethylase activity were significantly increased (P&nbsp;&lt; 0.05) in the group receiving 50&nbsp;mg of silymarin per day and in the E2-treated control group, respectively. However, the modulations of the CYP enzymes played only a minor role in the overall estrogenic effect of silymarin. Histological and functional alterations in the OVX rats treated orally with silymarin for 30 days were consistent with those seen in E2-treated rats and were indicative of estrogenic effects of silymarin.

scholarly journals INFLUENCE OF THE COMBINATION OF PASAK BUMI ROOT AND PROPOLIS EXTRACTS ON PARASITEMIA LEVELS IN MICE INFECTED WITH PLASMODIUM BERGHEI

2019 ◽  
pp. 268-271
Author(s):  
NUR HASANAH ◽  
HENDRI ASTUTY
Keyword(s):  
Combination Therapy ◽  
Plasmodium Berghei ◽  
Malaria Infection ◽  
Positive Control ◽  
Control Group ◽  
Control Groups ◽  
Propolis Extract ◽  
Blood Smears ◽  
Significant Difference ◽  
Global Concern

Objective: Malaria infection remains a global concern due to increasing resistance to artemisinin-based combination therapy. This study examinedthe antimalarial effects of propolis extract alone and in combination with pasak bumi root extract.Methods: In the study, 30 mice were divided into six groups including two control groups, two groups of mice treated with propolis aloneat concentrations of 90 and 180 mg/kg body weight (BW), and two combination groups of mice treated with 90 or 180 mg/kg BW propolis incombination with 60 or 75 mg/kg BW pasak bumi, respectively. Plasmodium berghei 2% was injected into each mouse, and blood smears wereprepared after 8 days to assess parasitemia.Results: The results revealed no significant difference in parasitemia levels between the positive control and the two combination groups (p=0.136 and0.289, respectively). However, superior growth inhibition (GI) results were observed in the combination groups (97.97% and 97.83%, respectively)than in the propolis monotherapy groups, whereas better outcomes were observed in the positive control group (98.63% GI) than in the propolismonotherapy groups (23.88% and 51.66%, respectively).Conclusion: These results illustrate that combination therapy is superior to propolis monotherapy in inhibiting parasitemia.

scholarly journals Low-dose brain estrogen prevents menopausal syndrome while maintaining the diversity of the gut microbiomes in estrogen-deficient rats

2018 ◽  
Vol 315 (1) ◽  
pp. E99-E109 ◽  
Author(s):  
Sunmin Park ◽  
Da Sol Kim ◽  
Eun Seon Kang ◽  
Da Bin Kim ◽  
Suna Kang
Keyword(s):  
Normal Control ◽  
Gut Microbiome ◽  
Positive Control ◽  
Menopausal Symptoms ◽  
The Other ◽  
Control Group ◽  
Control Groups ◽  
Ovx Rats ◽  
Control Serum ◽  
17Β Estradiol

We evaluated the effects of intracerebroventricular administration (ICV) of brain estrogen and progesterone on menopausal symptoms and their effects on the secretion of follicle-stimulating hormone(FSH) and luteinizing hormone (LH) in estrogen-deficient rats. Three weeks after ovariectomy (OVX) or sham operation, OVX rats were given ICV infusions of either 17β-estradiol (4 μg/day; ICV-E), progesterone(0.8 μg/day; ICV-P), or vehicle (control) for 4 wk. OVX rats in the positive-control group were orally provided 150 μg 17β-estradiol·kg body wt−1·day−1. Sham rats had ICV vehicle infusion (normal-control). Serum 17β-estradiol levels of ICV-E and ICV-P groups were higher than the control group but much lower than the normal- and positive-control groups. Tail skin temperature was higher in the control group than the other groups. Serum FSH and LH levels were much higher in the control group than positive- and normal-control groups, but ICV-E and ICV-P lowered the levels similar to the normal-control treatment. ICV-E and ICV-P prevented the decreased energy expenditure in OVX rats. Homeostasis model assessment estimate of insulin resistance was lowered in the descending order of the control, positive-control, ICV-P, ICV-E, and normal-control treatments. The decreased bone mineral density was prevented by the positive-control, ICV-E, and ICV-P treatments. The control group exhibited decreased short-term memory and spatial memory compared with the other groups. Surprisingly, the control group exhibited a decreased richness of the gut microbiome compared with normal-control group, and ICV-E protected against the decrease the most. In conclusion, small amounts of brain estrogen and, to some extent, progesterone improved menopausal symptoms by decreasing serum FSH levels and maintaining the diversity of the gut microbiome in estrogen-deficient rats.

scholarly journals Effect of Aloe Vera Extract on Reducing Aflatoxin B1 in Eggs of Laying Hen and Egg Yolk Oxidative Stability

2021 ◽  
Vol 11 (5) ◽  
pp. 12680-12688
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Stability ◽  
Aflatoxin B1 ◽  
Aloe Vera ◽  
Egg Yolk ◽  
Positive Control ◽  
Laying Hen ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups

Aloe vera extract's effect on Aflatoxin B1 (AFB1) residue and yolk oxidative stability was examined in yolk samples. The results indicated that residue of AB1 in AF-Aloe (100 and 300 ppm) treated groups were lower than the control groups on day 14 and was not detected on day 28. The lipid peroxidation level in all groups was significantly (p<0.05) lower than the positive control group. The weight, production, and carotenoid of eggs were not statistically significant between the groups. These results indicate that Aloe vera extract can be effective in diminishing AFB1.

scholarly journals Evaluation of clinical, histology, TNF-α, and collagen expressions on oral ulcer in rats after treatment with areca nut and chrysanthemum oral gel

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 623
Author(s):  
Liza Meutia Sari ◽  
Zaki Mubarak ◽  
Dina Keumala Sari
Keyword(s):  
Triamcinolone Acetonide ◽  
Positive Control ◽  
Flowering Plant ◽  
Positive Control Group ◽  
Control Group ◽  
Areca Nut ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sprague Dawley Rats ◽  
Tnf Α

Background: Areca nut (Areca catechu Linn.) is the seed of the fruit of the oriental palm that is commonly used among Southeast Asian communities. Chrysanthemum (Dendrathema grandiflora) is a flowering plant originating from East Asia and dominantly grows in China. Both of these plants have strong antioxidant activities. To investigate the mechanism of their wound healing activities, we prepared areca nut and chrysanthemum polyethylene oral gel and performed several in vivo assays using Sprague–Dawley rats. Methods: Sprague–Dawley rats were divided into five groups: Negative control group (rats with base gel treatment), positive control group (rats treated with triamcinolone acetonide), F1 (treatment with 20% areca nut:80% chrysanthemum), F2 (treatment with 50% areca nut:50% chrysanthemum), and F3 (treatment with 80% areca nut:20% chrysanthemum). Traumatic ulcers were performed on the buccal mucosa of all experimental animals that received topical oral gel and triamcinolone acetonide twice a day for seven days. The clinical and histological characteristics were analyzed and scored. Results: During the six days, the ulcerated area receded linearly over time and was completely cicatrized in F2 and positive control group (Dependent t-test, p<0.05). There were significant increases in body weight in F2 and positive control groups. There were no significant differences between groups in histology examination (Kruskal Wallis test, p<0.05). The moderate score of TNF-α levels was seen in F2 and positive control groups (ANOVA/Tukey test). Similar results were seen in the collagenases assay. Conclusions: A balanced combination of areca nut and chrysanthemum extract in the oral gel can optimize the healing of traumatic oral ulcers in rats through the increase of TNF-α and collagen deposition.

Sign in / Sign up

Export Citation Format

Share Document