scholarly journals Effect of Aloe Vera Extract on Reducing Aflatoxin B1 in Eggs of Laying Hen and Egg Yolk Oxidative Stability

2021 ◽  
Vol 11 (5) ◽  
pp. 12680-12688
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Stability ◽  
Aflatoxin B1 ◽  
Aloe Vera ◽  
Egg Yolk ◽  
Positive Control ◽  
Laying Hen ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups

Aloe vera extract's effect on Aflatoxin B1 (AFB1) residue and yolk oxidative stability was examined in yolk samples. The results indicated that residue of AB1 in AF-Aloe (100 and 300 ppm) treated groups were lower than the control groups on day 14 and was not detected on day 28. The lipid peroxidation level in all groups was significantly (p<0.05) lower than the positive control group. The weight, production, and carotenoid of eggs were not statistically significant between the groups. These results indicate that Aloe vera extract can be effective in diminishing AFB1.

scholarly journals Evaluation of clinical, histology, TNF-α, and collagen expressions on oral ulcer in rats after treatment with areca nut and chrysanthemum oral gel

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 623
Author(s):  
Liza Meutia Sari ◽  
Zaki Mubarak ◽  
Dina Keumala Sari
Keyword(s):  
Triamcinolone Acetonide ◽  
Positive Control ◽  
Flowering Plant ◽  
Positive Control Group ◽  
Control Group ◽  
Areca Nut ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sprague Dawley Rats ◽  
Tnf Α

Background: Areca nut (Areca catechu Linn.) is the seed of the fruit of the oriental palm that is commonly used among Southeast Asian communities. Chrysanthemum (Dendrathema grandiflora) is a flowering plant originating from East Asia and dominantly grows in China. Both of these plants have strong antioxidant activities. To investigate the mechanism of their wound healing activities, we prepared areca nut and chrysanthemum polyethylene oral gel and performed several in vivo assays using Sprague–Dawley rats. Methods: Sprague–Dawley rats were divided into five groups: Negative control group (rats with base gel treatment), positive control group (rats treated with triamcinolone acetonide), F1 (treatment with 20% areca nut:80% chrysanthemum), F2 (treatment with 50% areca nut:50% chrysanthemum), and F3 (treatment with 80% areca nut:20% chrysanthemum). Traumatic ulcers were performed on the buccal mucosa of all experimental animals that received topical oral gel and triamcinolone acetonide twice a day for seven days. The clinical and histological characteristics were analyzed and scored. Results: During the six days, the ulcerated area receded linearly over time and was completely cicatrized in F2 and positive control group (Dependent t-test, p<0.05). There were significant increases in body weight in F2 and positive control groups. There were no significant differences between groups in histology examination (Kruskal Wallis test, p<0.05). The moderate score of TNF-α levels was seen in F2 and positive control groups (ANOVA/Tukey test). Similar results were seen in the collagenases assay. Conclusions: A balanced combination of areca nut and chrysanthemum extract in the oral gel can optimize the healing of traumatic oral ulcers in rats through the increase of TNF-α and collagen deposition.

scholarly journals Evaluation of clinical, histology, TNF-α, and collagen expressions on oral ulcer in rats after treatment with areca nut and chrysanthemum oral gel

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 623
Author(s):  
Liza Meutia Sari ◽  
Zaki Mubarak ◽  
Dina Keumala Sari
Keyword(s):  
Triamcinolone Acetonide ◽  
Positive Control ◽  
Flowering Plant ◽  
Positive Control Group ◽  
Control Group ◽  
Areca Nut ◽  
Sprague Dawley ◽  
Control Groups ◽  
Sprague Dawley Rats ◽  
Tnf Α

Background: Areca nut (Areca catechu Linn.) is the seed of the fruit of the oriental palm that is commonly used among Southeast Asian communities. Chrysanthemum (Dendrathema grandiflora) is a flowering plant originating from East Asia and dominantly grows in China. Both of these plants have strong antioxidant activities. To investigate the mechanism of their wound healing activities, we prepared areca nut and chrysanthemum polyethylene oral gel and performed several in vivo assays using Sprague–Dawley rats. Methods: Sprague–Dawley rats were divided into five groups: Negative control group (rats with base gel treatment), positive control group (rats treated with triamcinolone acetonide), F1 (treatment with 20% areca nut:80% chrysanthemum), F2 (treatment with 50% areca nut:50% chrysanthemum), and F3 (treatment with 80% areca nut:20% chrysanthemum). Traumatic ulcers were performed on the buccal mucosa of all experimental animals that received topical oral gel and triamcinolone acetonide twice a day for seven days. The clinical and histological characteristics were analyzed and scored. Results: During the six days, the ulcerated area receded linearly over time and was completely cicatrized in F2, F3, and positive control group (Dependent t-test, p<0.05). There were significant increases in body weight in F2 and positive control groups. There were no significant differences between groups in histology examination (Kruskal Wallis test, p<0.05). The moderate score of TNF-α levels was seen in F2 and positive control groups (ANOVA/Tukey test, p<0.05). In the collagenases assay, a high concentration of areca nut (F3) induced the abundance of collagen during the ulcer healing process. Conclusions: The combination of areca nut and chrysanthemum extract in the oral gel can optimize the healing of traumatic oral ulcers in Sprague-Dawley rats through the increase of TNF-α and collagen deposition.

scholarly journals Tissue injuries of wistar rats treated with hydroalcoholic extract of Sonchus oleraceus L.

2011 ◽  
Vol 47 (3) ◽  
pp. 605-613 ◽  
Author(s):  
Franciele Carla Prichoa ◽  
Silvane Souza Roman ◽  
Vanusa Manfredini
Keyword(s):  
Wistar Rats ◽  
Skin Lesions ◽  
Positive Control ◽  
Negative Control ◽  
Hair Follicles ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups ◽  
Sonchus Oleraceus ◽  
Experimental Group

The use of plant species is emerging as an important alternative in the treatment of injuries. Therefore, the extract of Sonchus oleraceus 10% was employed in the repair of skin lesions. A total of 36 male Wistar rats were subjected to a punch injury and divided into three groups: a negative control, receiving no treatment, a positive control, treated with Dersani, and the experimental group treated with the extract. The injury was assessed macroscopically and microscopically. Morphometric data was collected at the 3rd, 5th and 7th postoperative day, and the experimental group showed greater changes in shrinkage of the lesion compared to control groups. On the 3rd postoperative day, the injury in the experimental group showed less necrotic tissue, lower slough and more granulation tissue in relation to the positive control group. On the 7th and 10th postoperative day, the injury in the experimental group showed lower slough compared to the positive control group. Microscopic analysis of lesions on the 5th postoperative day revealed increased fibroplasia in the experimental group compared to control groups, while on the 14th postoperative day less neovascularization was evident in the experimental group and increased formation of hair follicles in the negative control group. The extract of S. oleraceus provided tissue repair in accordance with normal physiological patterns thus confirming empirical evidence for its use.

scholarly journals Chitosan nanoparticle vaccine loaded with crude extracellular proteins of C. perfringens and Salmonella flagella is partially protective against necrotic enteritis in broiler chickens

2020 ◽  
Author(s):  
Gabriel Akerele ◽  
Nour Ramadan ◽  
Muhammed Mortada ◽  
Revathi Shanmugasundaram ◽  
Sankar Renu ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Positive Control ◽  
Negative Control ◽  
Extracellular Proteins ◽  
Positive Control Group ◽  
Economic Losses ◽  
Control Group ◽  
Necrotic Enteritis ◽  
Mrna Levels ◽  
Control Groups

AbstractNecrotic enteritis (NE) causes significant economic losses and food shortages world-wide. There are currently no licensed commercial vaccines against NE in broilers. Chitosan nanoparticles were formulated with extracellular proteins of C. perfringens surface-tagged with Salmonella flagellar proteins. One-day-old male broiler chicks were completely randomized to 3 treatments: Non-vaccinated non-challenged as negative control, Vaccinated-challenged, and non-vaccinated challenge as positive control. On day of hatch, d7, and d14 post-hatch, vaccinated-challenged birds were orally gavage with 50μg vaccine in 0.5ml PBS while positive control birds were gavage with 0.5ml PBS only. Birds in the vaccinated-challenged and positive control groups were orally infected on d14 post-hatch, with 5,000 oocysts/bird of E. maxima, followed by log 8 CFU of a virulent strain of C. perfringens on d19, d20, and d21 post-hatch. From d14 to 21 and d14 to 28 post-hatches, mortality in the vaccinated-challenged group was higher than that in the positive control group, approaching statistical significance (p=0.07). On d21 post-hatch, the mean lesion score of 3 birds/cage in the vaccinated-challenged group was higher than the positive control group, approaching statistical significance (p = 0.05). From d 14 to 28 post-hatch, the feed intake was higher and feed conversion ratio lower in the vaccinated-challenged group compared to the positive control group (p<0.05). On d21 post-hatch, antigen specific recall proliferation in the vaccinated-challenged group was higher than that in the negative and positive control groups (p<0.05). On d21 post-hatch, cecal tonsils CD8+ T lymphocytes expression in the vaccinated-challenged group was similar to the negative control group (p>0.05) but higher than that in the positive control group (p<0.05). Finally, vaccination resulted in an increase in ileal mRNA levels of zonula occluding on d21 post-hatch. In conclusion, there were numerical but not statistically significant decrease in NE lesions and mortality in vaccinated and challenged broilers. Further studies are needed to improve the efficacy of the vaccine and understand the mechanism underlying protection in vaccinated birds.

scholarly journals The effectiveness of Boiled figs leaf (FIcus Carica L) and Rosy Periwinkle (Catharantus roseus) on SGOT and SGPT levels of male wistar strain rats with hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 850-859
Author(s):  
Debora Oktaviani
Keyword(s):  
Treatment Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups ◽  
Wistar Strain ◽  
Significant Difference

Hepatitis is an inflammatory disease of liver cells that is contagious in the low category, the cause of it is bacteria, viruses, drugs and alcohol. The purpose of this study was to determine the effectiveness of boiled figs leaf (Ficus Carica L) and Rosy periwinkle (Catharantus roseus ) in reducing SGOT and SGPT levels. The subjects of this study were 30 male Wistar strain rats with age of 2-3 months, body weight 180-200 grams. Rats were divided into 3 groups; treatment groups, negative control groups and positive control groups. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. The treatment group was given 0.4 grams boiled figs leaf (Ficus Carica L) and 2.6 grams boiled rosy periwinkle  (Catharantus roseus) it given as much as 3.6 cc / day orally for 7 days. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare SGOT and SGPT levels. The results showed there were significant differences in SGOT levels between the treatment group, the positive control group, and the negative control group (p <0.05) and there was a significant difference in the SGPT value between the treatment group and the positive control group (p <0.05), while the negative control group and the treatment group there was no significant difference (p = 0.75). The conclusion of this study was the water extraction of figs leaf (Ficus Carica L), Rosy periwinkle (Catharantus roseus) had an effect in decreasing the SGOT and SGPT values of male wistar strain rats induced by paracetamol.

scholarly journals Resistance to Fracture of Dental Roots Obturated with Different Materials

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Berkan Celikten ◽  
Ceren Feriha Uzuntas ◽  
Kamran Gulsahi
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

scholarly journals Antidiabetic and antihyperlipidemic activities of Cucumis melo var. momordica fruit extract on experimental animals

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Arvind Kumar Srivastava ◽  
Alok Mukerjee ◽  
Abhishek Tripathi
Keyword(s):  
Blood Glucose ◽  
Cucumis Melo ◽  
Biological Properties ◽  
Positive Control ◽  
Positive Control Group ◽  
Public Health Issue ◽  
Control Group ◽  
Fruit Extract ◽  
Experimental Animals ◽  
Glucose Levels

Abstract Background Diabetes mellitus is a major public health issue related to the irregular metabolism of carbohydrates, protein, and fat. It occurs due to insufficient insulin production and insulin action. Cucumis melo possesses several biological properties including antioxidant, anti-inflammatory, antibacterial, antihypothyroidism, and antiangiogenic activities. The objective of the present study was to determine the antidiabetic and antihyperlipidemic activities of Cucumis melo var. momordica fruit extract on experimental animals. Result Results show that treatment with C. melo fruit extract and fraction caused a reduction in blood glucose levels. Cucumis melo toluene fraction (CMTF) exhibited a significant (*P < 0.05) reduction of blood glucose level on the 28th day, i.e., 122 mg/dL, in comparison with the positive control group (streptozotocin (STZ)). However, the extract of C. melo showed less significant results in comparison with CMTF. Triglyceride, LDL, and VLDL levels were increased chronically due to STZ and were significantly (*P < 0.05) restored to 84.16, 86.97, and 19.73, respectively, by CMTF in comparison with the positive control group (STZ in the dose of 55 mg/kg). The extract-treated groups also showed similar results as CMTF, but their efficacy was lesser than CMTF. Conclusion It is can be concluded that C. melo fruits can be used as an effective antidiabetic and antihyperlipidemic drug. Graphical abstract

Protective effect of Saccharomyces cerevisiae in Rattus norvegicus Ischemic Stroke Model

2021 ◽  
pp. 5785-5789
Author(s):  
Dedy Budi Kurniawan ◽  
Mokhamad Fahmi Rizki Syaban ◽  
Arinal Mufidah ◽  
Muhammad Unzila Rafsi Zulfikri ◽  
Wibi Riawan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ischemic Stroke ◽  
Brain Tissue ◽  
Tissue Injury ◽  
Intervention Group ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Hematopoietic Stem ◽  
The Brain

Stroke is one of the leading causes of morbidity and mortality in all ages. Ischemic stroke activates excitotoxic glutamate cascade leading to brain tissue injury. Saccharomyces cerevisiae is a unicellular yeast widely found in nature. S. cerevisiae is neuroprotective and able to increase the differentiation of hematopoietic stem cells (HSCs) into neuronal cells. it may increase levels of neuroprotectant BDNF in the brain tissue, therefore increase the protection of neurons. BDNF may prevent glutamate-driven excitotoxicity by reducing glutamate levels. This study uses a randomized post-test only controlled group design. In this in vivo study, rodent models of ischemic stroke were divided into five groups comprising of the negative control group, positive control group, intervention group 1 (18mg/kgBW), intervention group 2 (36mg/kgBW) and intervention group 3 (72 mg/kgBW). Groups treated with Saccharomyces cerevisiae extract showed significantly increased BDNF levels in the brain tissue, and the expression of the glutamate level was significantly reduced (P <0.05) compared to the positive control group. Thus Saccharomyces cerevisiae has a promising potential to become a therapy against ischemic stroke disease. however further research is needed regarding the efficacy and toxicity of Saccharomyces cerevisiae.

scholarly journals Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4) in rats

2009 ◽  
Vol 24 (5) ◽  
pp. 383-386 ◽  
Author(s):  
Cecília Maria de Carvalho Xavier Holanda ◽  
Monique Batista da Costa ◽  
Natália Chilinque Zambão da Silva ◽  
Maurício Ferreira da Silva Júnior ◽  
Vanessa Santos de Arruda Barbosa ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Aloe Vera ◽  
Treated Group ◽  
Control Group ◽  
Blood Levels ◽  
Control Groups ◽  
High Increase ◽  
Tropical Plant ◽  
And Control ◽  
The Brain

PURPOSE: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na99mTcO4 and laboratorial parameters in Wistar rats. METHODS: Twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na99mTcO4 by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (%ATI/g) was determined. Biochemical dosages were performed. RESULTS: There was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p<0.05). CONCLUSION: The aqueous extract of Aloe vera facilitated the uptake of Na99mTcO4 in organs of rats and it was responsible to a high increase of levels of AST and ALT.

scholarly journals Screening of natural product extracts for fly repellent and larvicide

2021 ◽  
Vol 20 (1) ◽  
pp. 25-40
Author(s):  
Kanokwan Bootyothee ◽  
◽  
Surasit Aunpromma ◽  
Atchara Artchayasawat ◽  
Opal Pitaksakulrat ◽  
...  
Keyword(s):  
Natural Products ◽  
Mortality Rate ◽  
Crude Extract ◽  
Rural Areas ◽  
Inorganic Compounds ◽  
Food Contamination ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Significant Difference

Fly is one of the vectors of foodborne pathogenic vectors and causes myiasis in humans and animals. To prevent the contamination of food and myiasis, various chemical products are commonly used. However, eco-friendly is now a trend all over the world so natural products are the alternative ways to reduce the chemical residue in the environment. The present study was screened the 12 natural products in 4 groups; the essential oil plants, the alkaloid plants, the cyanogenic plant, and the inorganic compounds which use a simple extraction on the fly repelling and larvicide. The fly repelling efficacy with 2 criteria consist of the time of the first swarm and percent repelling (PR) in the first 15 min. The larvicidal efficacy was determined by the mortality rate within 24 h. The Kaffir lime peels aqueous crude extract exhibited the best result on fly repelling from both criteria with no significant difference. The bamboo shoots aqueous crude extract gave the best result on larvicide with significantly different from other groups (P < 0.001) which was faster than the positive control group (Coumaphos & Propoxur). Moreover, the high mortality rate reached 73.33 ± 15.28% within 24 h, higher than the positive control group. The present study indicates the power of common natural products on fly repellent and larvicide that may apply for fly repellant to reduce the food contamination especially fresh materials and prevention or treatment of animal and human myiasis in rural areas

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