In vivo antioxidant potentials of Piliostigma thonningii (Schum) leaves: Studies on hepatic marker enzyme, antioxidant system, drug detoxifying enzyme and lipid peroxidation

2010 ◽  
Vol 30 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Taofeek Olakunle Ajiboye
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Carbon Tetrachloride ◽  
Oxidative Damage ◽  
Antioxidant Enzyme ◽  
Albino Rats ◽  
Marker Enzyme ◽  
Quinone Oxidoreductase ◽  
Piliostigma Thonningii

In this study, the in vivo antioxidant potentials of Piliostigma thonningii were investigated in carbon tetrachloride-induced hepatic and oxidative damage in rat. Albino rats were grouped into six, group A rats received sterile distilled water for 14 days while B rats received 0.5 mL/kg body weight of carbon tetrachloride intraperitoneally on day 14. Group C rats received 200 mg/kg body weight of Piliostigma thonningii leaves extract for 14 days. Groups D, E and F rats were pretreated with 50, 100 and 200 mg/kg body weight of Piliostigma thonningii leaves for 14 days and challenged with 0.5 mL/kg body weight of carbon tetrachloride on day 14. The extract treatment significantly attenuated both the decrease and the increase in liver and serum marker enzyme. Antioxidant enzyme activity as well as level of uridyl diphosphoglucuronosyl transferase, quinone oxidoreductase and glutathione S-transferase was significantly induced. There was attenuation of malonidialdehyde and lipid hydroperoxide increase. On the basis of the available data in this report, it can be postulated that Piliostigma thonningii leaves protect liver against hepatic and oxidative damage by carbon tetrachloride possibly by acting as an in vivo free radical scavenger, induction of antioxidant enzymes, drug detoxifying enzymes and prevention of excessive stimulation of antioxidant enzyme and lipid peroxidation.

scholarly journals Oxidative stress and lipid peroxidation with exposure of emerging disinfection byproduct 2,6-dichlorobenzoquinone in mice

2021 ◽  
Author(s):  
Shi-Wei Li ◽  
Ming-Hui Chang ◽  
Wen-Jun Zhao ◽  
He-Lian Li ◽  
Hong-Jie Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Heme Oxygenase 1 ◽  
Quinone Oxidoreductase ◽  
Disinfection Byproduct ◽  
Adult Mice ◽  
Stress Damage ◽  
The Impact

Abstract 2,6-dichlorobenzoquinone (2,6-DCBQ) is an emerging disinfection byproduct frequently detected in drinking water. Previous studies have indicated that 2,6-DCBQ causes oxidative stress damage in some live systems, but this has yet to be tested in vivo in mammals. In the present study, adult mice were exposed to 2,6-DCBQ for 30 d via gavage at 0 ~ 100 mg kg− 1 with the responses of antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT]), key oxidative stress response genes (Heme oxygenase-1 [HO-1], NADPH quinone oxidoreductase 1 [NQO1] and glutamate-L-cysteine ligase catalytic subunit [GCLC]) in the Nrf2-keap1 pathway, and lipid peroxidation (malonaldehyde, MDA) as an indicator of oxidative damage being measured. Our results indicated that 2,6-DCBQ decreased the activities of SOD and CAT, repressed transcription of key genes in the Nrf2-keap1 pathway, and caused measurable oxidative damage. These results reveal the impact of 2,6-DCBQ in a model mammalian system and are key to understanding the potential impacts of 2,6-DCBQ in humans.

scholarly journals Hepatoprotective effects of ethanol extract of Caesalpiniabonduc against carbon tetrachloride induced hepatotoxicity in Albino Rats

2016 ◽  
Vol 20 (2) ◽  
pp. 396-401
Author(s):  
A.E. Ubhenin ◽  
I Igbe ◽  
F.A. Adamude ◽  
A Falodun
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Serum Marker ◽  
Albino Rats ◽  
Different Doses

The present study was carried out to evaluate the Acute toxicity, hepatoprotective and in-vivo antioxidant activities of ethanolic extract of Caesalpinia bonduc leaf on carbon tetrachloride (CCl4) induced liver damage using Swiss albino rats . The ethanolic extract of the plant of C. bonduc were suspended in 5 % tragacanth and then administered orally at doses of 250 and 500 mg/kg body weight for fourteen days before intraperitoneally injection of Carbon tetrachloride (CCl4) at dose of 2 mL/kg body weight. The plant extracts at 250 and 500mg/kg b.wt showed a remarkable hepatoprotective and invivo antioxidant activities against carbon tetrachloride CCl4 – induced hepatotoxity judged from the serum marker enzymes .The CCl4 induced significant increase in aspartate amino transferase( AST), alanine amino transferase (ALT), alkaline phophatase (ALP), total bilirubin, and malondyaldehyde (MDA) with a reduction of total protein, catalase, and glutathione peroxidase . Treatment of rats with different doses of plant extract (250 and 500 mg/kg b.wt.) significantly (P< 0.001) altered serum maker enzymes and antioxidant levels to near normal levels. The study suggests that C. Bonduc specifically chloroform and ethyl acetate fraction may be good sources of natural antioxidant and hepatoprotective substance.Keywords: Ceasalpiniabonduc, hepatoprotective, intraperitoneally , antioxidant

scholarly journals Antidiabetic and antiulcerative potential of Garcinia lanceifolia Roxb. bark

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Nilutpal Sharma Bora ◽  
Partha Sarathi Bairy ◽  
Abdus Salam ◽  
Bibhuti Bhusan Kakoti
Keyword(s):  
Body Weight ◽  
Serum Levels ◽  
Northeast India ◽  
Scientific Data ◽  
Antidiabetic Activity ◽  
Histological Evaluation ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Dose Dependent

Abstract Background Garcinia lanceifolia Roxb. has been used by many ethnic communities of Northeast India to mitigate various disorders like dyspepsia, ulcers, diabetes, etc. However, a robust scientific study on its antidiabetic and antiulcer potential is unavailable till date. The aim of this present study is to scientifically validate if the antidiabetic and antiulcer effects reported by the ethnic tribes of Assam has any scientific value or not. The effects were tested in adult Wistar albino rats using approved animal models for preclinical testing of pharmacological activities. Results The hydroalcoholic extract of the bark of Garcinia lanceifolia Roxb. was prepared and its LD50 was calculated. The LD50 was determined to be greater than 5000 mg/kg body weight. The extract at doses of 250 mg/kg body weight and 500 mg/kg body weight was found to exhibit a very potent dose-dependent antidiabetic activity. The results were backed by a battery of test including analysis of serum levels of blood glucose, lipid profiles, in vivo antioxidant enzymes, and histopathological studies. Evidence of dose-dependent antiulcer activity of the extract was backed by robust scientific data. It was found that HAEGL induced a significant dose-dependent increase in the ulcer index in both alcohol-induced and acetic acid-induced ulcer models, which was evident from the macroscopic observation of the inner lining of the gastric mucosa and the histological evaluation of the extracted stomach. Conclusion The results suggested that the bark of Garcinia lanceifolia (Roxb.) has significant antidiabetic and antiulcer potential. Further studies with respect to the development herbal dosage forms and its safety evaluation are required.

Antioxidant enzyme activities and lipid peroxidation in Mytilus galloprovincialis from the French Mediterranean coast

2010 ◽  
Vol 39 (4) ◽  
Author(s):  
Krzysztof Gwoździński ◽  
Marta Gonciarz ◽  
Ewa Kilańczyk ◽  
Aleksandra Kowalczyk ◽  
Anna Pieniążek ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Antioxidant Enzyme ◽  
Digestive Gland ◽  
Enzyme Activities ◽  
Mytilus Galloprovincialis ◽  
Mediterranean Coast ◽  
Antioxidant Enzyme Activities ◽  
Gland Tissue ◽  
Two Populations

Antioxidant enzyme activities and lipid peroxidation inIn the present work we have studied some of the indicators of oxidative damage of the digestive gland tissue of two populations of mussels

scholarly journals Protective effects of honey and bee venom against lipopolysaccharide and carbon tetrachloride-induced hepatoxicity and lipid peroxidation in rats

2020 ◽  
Vol 9 (5) ◽  
pp. 693-705
Author(s):  
Noha M Meligi ◽  
Suzan Alaa Ismail ◽  
Nagy S Tawfik
Keyword(s):  
Lipid Peroxidation ◽  
Carbon Tetrachloride ◽  
Hematological Parameters ◽  
Lipid Peroxide ◽  
Alpha Fetoprotein ◽  
Bee Venom ◽  
Albino Rats ◽  
Average Weight ◽  
Protective Effects ◽  
Liver Histopathology

Abstract In the present study, the protective effects of honey and bee venom (BV) either independently or in combination against lipopolysaccharide (LPS) and carbon tetrachloride (CCl4)-induced hepatoxicity, lipid peroxidation, and hematological alterations in male albino rats were investigated. In addition, histopathological alterations of hepatic tissues induced by LPS/CCL4 were recorded. Sixty-four of male albino rats of average weight 120–150 g were included in this study. Rats were divided into eight equal groups of eight. The obtained results demonstrated that treatment with LPS/CCl4 caused an increase in the levels of alpha-fetoprotein, which was accompanied by changes in the hepatic function biomarkers that characterized by the increased levels of transaminases (AST, ALT). The results showed oxidative stress as assigned by the increase in lipid peroxide. Meantime detraction in the antioxidants, including glutathione peroxidase was observed. Interruptions in biochemical parameters accompanied by disturbances in hematological parameters and liver histopathology were resulted due to exposure to LPS/CCl4. This study showed the use of honey and BV provided a protective effect on hepatotoxicity induced by LPS/CCl4. This might have been occurred through the reduction of hepatic transaminases and the “Alpha-fetoprotein” in serum and the equilibration of the antioxidation system, thereby, inhibiting the reactive oxygen species accumulation. Honey and BV administration reestablish disturbed hematological parameters and liver histopathology persuaded by LPS/CCl4. More interesting, we demonstrated that using a combination of the honey and BV showed promising enhancement in their protective effects over the use of just one of the two reagents.

scholarly journals Hemidesmus indicus Protects against ethanol-induced liver toxicity

2008 ◽  
Vol 13 (1) ◽  
Author(s):  
Nadana Saravanan ◽  
Namasivayam Nalini
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Weight Gain ◽  
Enzyme Activities ◽  
Liver Glycogen ◽  
Root Extract ◽  
Marker Enzyme ◽  
Hemidesmus Indicus ◽  
Ft Ir ◽  
G Ratio

AbstractAlcoholic liver disease (ALD) is one of the most common diseases in modern society. A large number of studies are in progress aiming to identify natural substances that would be effective in reducing the severity of ALD. Although there are currently a number of drugs on the market, their long-term use can have numerous side effects. Hemidesmus indicus is an indigenous Ayurvedic medicinal plant used in soft drinks in India. In this study, we examined the effects of its ethanolic root extract on experimental liver damage in order to evaluate its hepatoprotective effects against hepatotoxicity induced in rats by ethanol at a dosage of 5 g/kg body weight for 60 days. The H. indicus root extract was given at a dose of 500 mg/kg body weight for the last 30 days of the experiment. The animals were monitored for food intake and weight gain. The liver was analysed for the degree of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) and antioxidant status using the activities of glutathione-depedendant enzymes. The degree of liver damage was analysed using serum marker enzyme activities, the total protein, albumin, globulin, ceruloplasmin and liver glycogen contents, and the A/G ratio. The Fourier transform infrared spectra (FT-IR) of the liver tissues were recorded in the region of 4000–400 cm−1. The ethanol-fed rats showed significantly elevated liver marker enzyme activities, lipid peroxidation levels and reduced antioxidant levels as compared to the control rats. Oral administration of H. indicus for the latter 30 days resulted in an increased food intake and weight gain, decreased TBARS levels, near normal levels of glutathione-dependent enzymes, increased total protein, albumin, globulin and liver glycogen contents, an increased A/G ratio, and decreased liver marker enzyme activities and ceruloplasmin levels. The relative intensity of the liver FT-IR bands for the experimental groups were found to be altered significantly (p < 0.05) compared to the control samples. For the group that had H. indicus co-administered with ethanol, the intensity of the bands was near normal. Moreover, the results of the FT-IR study correlated with our biochemical results.

scholarly journals Nephroprotective acitvity of Amomum subulatum seeds against cypermethrin induced nephrotoxicity in rats

2016 ◽  
Vol 5 (4) ◽  
pp. 145-149
Author(s):  
Goutham Sagarkatte Puttanna ◽  
◽  
Swarnalatha Nayak ◽  
Mundugaru Ravi ◽  
B Ravishankar ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Normal Control ◽  
Elevated Serum ◽  
Kidney Tissue ◽  
Seed Extract ◽  
Tissue Homogenate ◽  
Albino Rats ◽  
Test Drug ◽  
Amomum Subulatum

The objective of the present study was to evaluate nephroprotective activity of Amomum subulatum seed extract against Cypermethrin induced nephrotoxicity in albino rats. The animals were divided into five different groups consisting of six rats each. Except normal control and test drug alone group, all the rats were treated with Cypermethrin 25g/kg body weight orally for 28 consecutive days and test group IV & V (ASSE 200 & 400mg/kg body weight) were co administered with Cypermethrin orally for 28 consecutive days. On the 28th haematalogical, biochemical and histopathalogical parameters were estimated. The chronic administration of Cypermethrin caused significant elevation in the serum creatinine and urea level and increased lipid peroxidation in the kidney tissue homogenate as compared to normal control. The histological examination of kidney tissues revealed mild fatty changes especially in the sub capsular region in sections, dilatation of glomerulus along with obliteration of periglomerular space and shrinkage of glomerulus was observed. The co administration of test drug significantly attenuated the Cypermethrin induced elevated serum urea and creatinine and lipid peroxidation in tissue homogenate. Thus we can conclude the Amomum subulatum seed extract has significant nephroprotective effect and reversed Cypermethrin induced nephrotoxicity.

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