Oral microbial colonization in patients with systemic lupus erythematous: correlation with treatment and disease activity

Lupus ◽  
2012 ◽  
Vol 21 (9) ◽  
pp. 969-977 ◽  
Author(s):  
EAF de Araújo Navas ◽  
EI Sato ◽  
DFA Pereira ◽  
GN Back-Brito ◽  
JA Ishikawa ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Immunosuppressive Drugs ◽  
Inactive Disease ◽  
Control Group ◽  
Healthy Individuals ◽  
Systemic Lupus ◽  
Microbial Counts ◽  
Pseudomonas Spp ◽  
Oral Rinse ◽  
And Control

Treating patients with systemic lupus erythematosus (SLE) with steroids and immunosuppressive drugs may interfere in the presence of potentially opportunistic microorganisms in the oral cavity. The aim of this study was to evaluate the presence of Candida spp., Staphylococcus spp., Enterobacteria and Pseudomonas spp. in the oral cavity of SLE patients, compared with healthy controls. A group of 40 patients who had received therapy for at least 60 days was selected (19–53 years). For the control group, 40 healthy individuals matched for age, gender and use of partial prosthesis were selected. Oral rinse samples were collected and plated on specific culture media. After incubation, the number of colony forming units (CFU) was obtained and the isolates were identified at species level. Microbial counts were compared between SLE and control by analysis of variance (ANOVA) and Mann–Whitney ( p < 0.05 significant). Microorganism counts in patients with and without immunosuppressive drugs, as well with active and inactive disease (according to SLEDAI score) were also compared. No significant differences in CFU/mL between SLE and control patients were observed (yeasts, p = 0.55; Staphylococci, p = 0.24; Enterobacteria/ Pseudomonas spp., p = 0.26). No differences in microbial counts were observed regarding clinical parameters tested. The most frequent species isolated in the SLE group were Candida albicans, Staphylococcus epidermidis and Klebsiella oxytoca. In conclusion, no differences in frequency and microorganism levels were found between SLE patients and healthy individuals.

scholarly journals NOSOGENIC DEPRESSION IN PATIENTS WITH PATHOLOGY OF THE MUCOUS ORAL CAVITY

2020 ◽  
Vol 15 (4) ◽  
pp. 50-54 ◽  
Author(s):  
Karen Karakov ◽  
Tatyana Vlasova ◽  
Artur Oganyan ◽  
Araksia Khachaturyan ◽  
Svetlana Karakova ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Oral Cavity ◽  
Treatment Regimen ◽  
Oral Mucosa ◽  
New Technologies ◽  
Well Being ◽  
Diagnostic Methods ◽  
Control Group ◽  
Traditional Treatment ◽  
And Control

Importance. Despite the improvement of diagnostic methods and the introduction of new technologies in the treatment of pathology of the oral mucosa, it is difficult to achieve stable remission, since 70 % of cases are based on somatic and bodily suffering factors. The article presents data from a comparative analysis of the use of the traditional treatment regimen for patients with pathology of the oral cavity and the inclusion of the latest generation of antipsychotics in the treatment regimen. It was established that, when applied, a tendency towards normalization of psychological and autonomic status was observed. The inclusion of anxiolytics and antioxidants in the complex of general therapy for patients with pathology of the oral mucosa contributed to a more pronounced positive dynamics of achieving and prolonging the phase of remission. Objectives — to evaluate the effectiveness of introducing drugs of the latest generation of antipsychotics into the traditional treatment regimen for patients with pathology of the oral cavity. Methodology. Under our supervision, there were 30 patients aged 40 to 60 years with a diagnosis of pathology of the oral mucosa and pathology of the periodontium. All examined were divided into 2 groups: the main (15 people) and control (15 people). Results. Clinical well-being, manifested in a subjective decrease in pain in the tongue, feelings of rawness, tingling, numbness, awkwardness, in the tongue, was noted already at the 5th visit in 67 % of patients of the main group, while in the control group in 17 % of patients. Conclusions. The inclusion of anxiolytics and antioxidants in the complex of general therapy for patients with pathology of the oral mucosa contributed to a more pronounced positive dynamics of achieving and prolonging the phase of remission. Our data allow us to implement this treatment regimen in practical healthcare.

scholarly journals EFFECT OF HERBAL TOOTHPASTE CONTAINING NEEM LEAVES EXTRACT (AZADIRACHTA INDICA) AGAINST GINGIVITIS: A CLINICAL STUDY

2019 ◽  
pp. 117-119
Author(s):  
AMELIA PRISCILLA SUGIARTA ◽  
ROBERT LESSANG ◽  
NATALINA .
Keyword(s):  
Clinical Study ◽  
Oral Cavity ◽  
Azadirachta Indica ◽  
Test Group ◽  
Tooth Brushing ◽  
Plaque Index ◽  
Control Group ◽  
Leaf Extracts ◽  
Neem Leaves ◽  
And Control

Objective: Neem leaves are known to have antibacterial, anti-inflammatory, antifungal, antiseptic, antitumor, antihyperglycemic, antiulcer, andantiviral effects. In addition, they can reduce the counts of plaque-forming bacteria in the oral cavity. This study aimed to evaluate the effect of anherbal toothpaste containing neem leaves extract against gingivitis.Methods: A total of 40 subjects were divided into two groups (n=20 each): Experimental and control. Those in the test group were instructed to brushtheir teeth twice a day for 7 days with neem leaves extract toothpaste, whereas the control group subjects were asked to continue with the non-herbalpaste they used for tooth brushing. Plaque index (PI) and papillary bleeding index (PBI) were measured on day 0 and day 7.Results: Significant reductions in PI and PBI were noted between the test and control groups.Conclusion: The findings of this clinical study provide evidence that neem leaf extracts are effective in reducing gingivitis.

scholarly journals Features of the cytokine profile and the effectiveness of radical treatment of focal infection in patients with psoriasis

2021 ◽  
Vol 97 (2) ◽  
pp. 32-40
Author(s):  
Aleksandr V. Patrushev ◽  
Alexey V. Samtsov ◽  
Alexey V. Soukharev
Keyword(s):  
Randomized Study ◽  
Total Duration ◽  
Disease Recurrence ◽  
Cytokine Profile ◽  
Control Group ◽  
Healthy Individuals ◽  
Radical Treatment ◽  
Focal Infection ◽  
Risk Of Disease ◽  
And Control

Background. Focal infection (FI) are important trigger factors for the development of psoriasis, which means they can aggravate the course of dermatosis, including due to an increase in certain pro-inflammatory cytokines in the blood. Aim. To study the cytokine profile of patients with psoriasis in the progressive period of the disease, as well as to assess the effect radical treatment of focal infection on the course of dermatosis. Material and methods. Prospective comparative non-randomized study included 52 patients with psoriasis vulgaris, aged 18 to 65 years, who signed a informed consent. The first group consisted of patients who underwent radical treatment FI (16 people), the second those who did not treat FI (16 people), and the third group consisted of patients in whom FI was not detected (20 people). Cytokine concentrations were measured in the progressive stage of the disease in 52 patients with psoriasis and 20 individuals in the control group (healthy individuals).The spectrum of detected cytokines included: IL-1, IL-2, IL-4, IL-6, IL-8, IL-10, IL-17A, as well as INF- and INF-. For the detection of all cytokines, except for IL-17A, reagents from Vector-Best, Russia were used. The Human ELISA MAX Deluxe Set (BioLegend, USA) was used to determine the concentration of IL-17A. During the main course of treatment (0, 2 and 4 weeks), as well as the follow-up of patients (12, 24 and 52 weeks), the severity of psoriasis was assessed with the calculation of the PASI index. At the end of the study (week 52), the number of relapses and the total duration of remission were recorded. Results. The level of IL-8 was increased in 90,6% of psoriasis patients with FI. In this case, significant differences were obtained in comparison with the group of patients with psoriasis without diagnosed FI and with the control group, in which an increase in the concentration of IL-8 was noted, respectively, in 65% and 30% of cases. A moderate positive correlation was found between the IL-8 level and the PASI index (rs = 0,48; p = 3,6 104). The levels of INF- and IL-6 in psoriasis patients with FI were increased, respectively, in 15,6% and 21,9% of cases and significantly differed from the group of practically healthy individuals (p 0,05), differences from the group of patients with psoriasis without FI was not identified. The concentration of TNF- did not differ in all three groups. The level of IL-17A was significantly increased in comparison with practically healthy individuals both in the group of psoriasis patients with FI and in the group of patients without FI (Me = 3,3 and 4,3 pg/ml versus 0,2 pg/ml). The concentrations of five interleukin cytokines, namely IL-1, IL-2, IL-4, IL-10 and INF-, did not exceed the upper limit of the reference range in all patients of the experimental (patients with psoriasis) and control groups. Evaluation of the PASI index during the observation of patients in the first and second groups showed significantly lower values of this indicator for weeks 24 and 52 in the first group (0,2 vs. 7,0; p = 0,02 and 0,1 vs. 7,2; p = 0,002). The relative risk of disease recurrence in the absence of radical treatment for FI was 2,6 (CI 1,1 to 5,2). Conclusion. Data were obtained showing the role of IL-8 and focal infection, with the presence of which its increased production is associated, in the worsening of the course of psoriasis with the frequent development of relapses. Important preventive measures leading to a decrease in the number of relapses of psoriasis are timely diagnosis and radical treatment of focal infection.

scholarly journals Study on Homocysteinemia as a Risk Factor of Ischemic Stroke

2021 ◽  
Vol 34 (1) ◽  
pp. 33-39
Author(s):  
Md Amjad Hossain Pramanik ◽  
Achinta Kumar Mallick ◽  
Mukul Kumar Sarkar ◽  
SM Emdadul Haque ◽  
Md Raseul Kabir ◽  
...  
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Ischemic Stroke ◽  
Independent Risk Factor ◽  
Homocysteine Level ◽  
Control Group ◽  
Age Group ◽  
Healthy Individuals ◽  
Serum Homocysteine ◽  
And Control

Despite recent advances, only two-third of all strokes can be attributed to known causal risk factors. Homocysteine (tHcy), a sulfur-containing amino acid, is now considered to be an important risk factor for vascular diseases, along with the established risk factors like hyperlipidemia, hypertension, diabetes mellitus, and smoking. Elevated homocysteine levels play a causal role in the pathogenesis of atherosclerosis, thromboembolism and vascular endothelial dysfunction with an increased incidence of ischemic stroke.  This study aimed to find out the association of hyperhomocysteinemia with ischemic stroke. A total of 100 subjects were included in this study, 50 were ischemic stroke patients enrolled as case, and 50 were normal healthy individuals enrolled as control. Serum homocysteine level was measured in both case and control groups. The comparison was made in both groups regarding other common risk factors like diabetes mellitus, hypertension, smoking, dyslipidemia, family history, etc.  Among 100 patients, 50 had ischemic stroke and 50 were healthy individuals. In this study, out of all patients, abnormal serum homocysteine level was found in 32% of cases and 12% of controls. The mean (±SD) serum homocysteine level was found 16.50±13.86 μmole/L in cases and 9.46±3.49 μmole /L in the control group. Significant (p<0.05) difference was found between the case and the control. The incidence of hyperhomocysteinemia is higher in ischemic stroke cases than that in age-sex-matched healthy controls. In our study, serum homocysteine was high in both younger age group patients (16.65±14.55 μmole/L vs. 9.52±3.19 μmole/L) and older age group patients (16.33±9.87 vs. 9.35±3.97 μmole/L,) in case and control group respectively. Significant (p<0.05) difference was found between the case and the control. Multiple logistic regression analysis showed that abnormal serum homocysteine is an independent risk factor of ischemic stroke. So we conclude that hyperhomocysteinemia is an important and independent risk factor for the development of ischemic stroke. Hypertension and smoking are important contributory to elevated serum homocysteine. TAJ 2021; 34: No-1: 33-39

scholarly journals Ringworm: Evaluation of C-reactive Protein and Lipid Profile as well as Their Correlation with the Patients Age and Body Mass Index

2020 ◽  
Vol 19 (4) ◽  
pp. 673-677
Author(s):  
Md Shafiul Hossen ◽  
Auditi Kar ◽  
Hasan Moinul Haque
Keyword(s):  
Lipid Profile ◽  
Total Cholesterol Level ◽  
Medical Science ◽  
Control Group ◽  
Healthy Individuals ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Significant Difference ◽  
Serum Crp ◽  
And Control

Objectives: Ringworm is a common cutaneous fungal disease with worldwide distribution.It may lead to the abnormal c-reactive protein levels and metabolism of lipid. Thus this study has been designed for determining serum CRP and also evaluating the Lipid profile in patients associated with ringworm. Materials and methods: This study recruited 25 patients with ringworm (cases) and 25 healthy individuals (controls). A questionnaire of personal characteristics was completed for each patient and control. Inflammatory marker (CRP) and Serum lipid profile (total, lowdensity lipoprotein (LDL), high-density lipoprotein (HDL) cholesterol and triglyceride) was recorded for each case and control. The test of significance was applied for statistical analysis. Results and discussion: The mean serum CRP were 1.608±0.14 and 0.648±0.03 mg/dl and total cholesterol level were 175.09±3.26 and 182.09±3.01 mg/dl in ringworm patients and control subject respectively. It was also observed that the serum TG; HDL; LDL levels were 131.76±3.07 and 139.84±3.47 mg/dl; 80.79±1.79 and 73.35±4.14 mg/dl; 67.95±4.64 and 86.55±3.70 mg/dl in patients and control group respectively. The value of CRP was significantly increased in patients compared to control group (p <0.05). There was no significant difference of lipid profile study between patients and control Conclusion: Findings of this study indicate increased concentrations of CRP in patients affected to ringworm and its role in this infection. Patients should thus be routinely screened for such abnormalities Probably observation of lipid profile in healthy individuals in compare with patients group indicates that it has no relationship in case of ringworm. Bangladesh Journal of Medical Science Vol.19(4) 2020 p.673-677

Increased ERK and JNK activities correlate with disease activity in patients with systemic lupus erythematosus

2009 ◽  
Vol 69 (01) ◽  
pp. 175-180 ◽  
Author(s):  
Y Molad ◽  
M Amit-Vasina ◽  
O Bloch ◽  
E Yona ◽  
M J Rapoport
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Disease Activity ◽  
Lupus Erythematosus ◽  
Map Kinases ◽  
Mononuclear Cells ◽  
Activity Index ◽  
Severe Disease ◽  
Inactive Disease ◽  
Control Group ◽  
Systemic Lupus

Background:Aberrant signalling along the p21ras/MAP kinase pathway has been demonstrated in systemic lupus erythematosus (SLE).Objective:To determine whether expression and activity of the MAP kinases ERK and JNK reflect disease activity in patients with SLE.Methods:Blood samples of 42 outpatients with SLE were prospectively collected during four consecutive visits. The control group included 20 healthy subjects. Disease activity was assessed using the SLE Disease Activity Index (SLEDAI). Expression of total ERK and JNK kinases and their active forms (pERK and pJNK) was determined in whole protein lysates of peripheral blood mononuclear cells.Results:The mean levels of the active kinases pERK and pJNK were significantly increased in patients with active disease (SLEDAI 4–20) as compared with patients with inactive disease (SLEDAI 0–3), p = 0.04, as well as with healthy controls, p = 0.03 and p = 0.003 for pERK and pJNK, respectively. The percentage of activated forms of ERK and JNK of the total expression of these MAP kinases was also gradually increased, reaching 50% for pERK and >40% for pJNK in patients with SLE with moderate-to-severe disease (SLEDAI 7–20), p = 0.005, p = 0.005 and p = 0.02, p = 0.05 as compared with controls and inactive patients, respectively. A decrease of more than three SLEDAI points was associated with a significant reduction in the expression of both total and activated forms of ERK and JNK, p = 0.03, p = 0.01, respectively.Conclusions:The results show that ERK and JNK activity reflects disease activity in patients with SLE. These MAP kinases may serve as additional tools for the evaluation of disease activity and management of these patients.

scholarly journals MAGNETIC RESONANCE CHARACTERISTICS OF THE NEUROVASCULAR RELATIONSHIP AS A PROBABLE PREDICTOR OF TRIGEMINAL NEURALGIA

2018 ◽  
Vol 99 (5) ◽  
pp. 231-236
Author(s):  
A. V. Bakunovich ◽  
V. E. Sinitsyn ◽  
E. A. Mershina
Keyword(s):  
Magnetic Resonance ◽  
Trigeminal Neuralgia ◽  
Posterior Cranial Fossa ◽  
Control Group ◽  
Slice Thickness ◽  
Healthy Individuals ◽  
Study Population ◽  
Cranial Fossa ◽  
And Control ◽  
Symptomatic Side

Objective.To validate MR-criteria used for diagnosis of trigeminal neuralgia (TN).Material and methods.Study population consisted of 133 patients: with trigeminal neuralgia (86, surgically (56) and clinically (30) verified; 55 ± 11 years) and control group (47; 51 ± 16 years). The data were obtained using the FIESTA sequence (slice thickness 0.5 mm) on 3T and 1.5T (Discovery 750w and Optima 450w, General Electric).Results.The diameters of the adjacent vessels on the symptomatic side were significantly bigger than the diameters on the asymptomatic side in patients with neuralgia (medians 1.2 and 1 mm, p<0.001). The shortest distances between nerves and vessels on the symptomatic side were significantly smaller than the distances on the asymptomatic side (medians 0 and 1.6 mm, p<0.001). The distances between points of closest neurovascular contact and pons on the symptomatic side were significantly smaller in comparison with the controls (medians 2.7 and 5.3 mm, p<0.001); the distances between the symptomatic and asymptomatic side were not statistically different (medians 2.7 and 3.9 mm, p=0.09) in this parameter. The lengths of closest neurovascular contact was bigger in patient with TN (medians 3 and 0 mm, p<0.05), as well as in comparison with the asymptomatic side (medians 3 and 0 mm, p<0.0001).Conclusion.Noninvasive MR-measurements of the posterior cranial fossa vessels show the differences between the symptomatic and asymptomatic side in the patients with TN as well as healthy individuals. This result may be used as a predictor of TN.

scholarly journals Hyperprolactenemia in Women with Systemic Lupus Erythematusus

2007 ◽  
Vol 4 (4) ◽  
pp. 617-621
Author(s):  
Baghdad Science Journal
Keyword(s):  
Lupus Erythematosus ◽  
Serum Prolactin ◽  
Control Group ◽  
Immunoradiometric Assay ◽  
Stage Versus ◽  
Active Stage ◽  
Systemic Lupus ◽  
Control Groups ◽  
And Control ◽  
Age And Sex

Recent accumulated evidences suggest that prolactin is an important immunomodulator and may have a role in the pathogenesis of systemic lupus erythematosus (SLE). The aim of this study was to assess the frequency of hyperprolactinemia in women with SLE and to evaluate its correlation with disease flares. Serum prolactin levels were measured in 62 women with SLE and 50 age- and sex-matched healthy controls. In patients and control groups prolactin levels were determined by immunoradiometric assay (IRMA). The prolactin level was found to be higher than normal rang in (40.3%) of SLE patients in active stage versus only (8.06%) of the same SLE patients but in the inactive stage and in (4%) of control group, the elevation was ranging between mild (72%), medium (20%) and high (8%). The study concludes that patients who having a flare - up had a trend to higher mean of prolactin (mild and medium) levels than inactive stage patients.

scholarly journals Circulating TCR γδ Cells in the Patients with Systemic Lupus Erythematosus

1999 ◽  
Vol 8 (6) ◽  
pp. 305-312 ◽  
Author(s):  
Ewa Robak ◽  
Jerzy Z. Błoński ◽  
Jacek Bartkowiak ◽  
Hanna Niewiadomska ◽  
Anna Sysa-Jędrzejowska ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Lupus Erythematosus ◽  
Γδ T Cells ◽  
Inactive Disease ◽  
Control Group ◽  
Systemic Lupus

Systemic lupus erythematosus (SLE) is a disorder with a wide range of immunological abnormalities. The results of the studies undertaken in the last decade indicated that SLE pathogenesis was mainly connected with the breakdown of the activation control of B and T cells, generating humoral or cell-mediated responses against several self-antigens of affected cells. The last studies demonstrate that the role of γδ T lymphocytes in autoimmune diseases can be especially important. Flow cytometry techniques were used to investigate the number and percentage of TCR γδ T cells and their most frequent subtypes in peripheral blood of 32 patients with SLE and 16 healthy volunteers. We also correlated TCR γδ cells number with the level of T CD3+, T CD4+, T CD8+, and NK (CD16) cells (cytometric measurements) and SLE activity (on the basis of clinical investigations). Our studies were preliminary attempts to evaluate the role of that minor T cell subpopulation in SLE. Absolute numbers of cells expressing γδ TCR in most SLE blood specimens were significantly lower than in the control group (P<0.006). However, since the level of total T cell population was also decreased in the case of SLE, the mean values of the percentage γδ T cells of pan T lymphocytes were almost the same in both analysed populations (7.1% vs 6.3%, respectively). In contrast to Vδ2+ and Vγ9+ subtypes of pan γδ T cells, Vδ3+ T cells number was higher in SLE patients (20×10 cells/μl) than in healthy control group (2×2 cells/μl) (P=0.001). However, we found no differences between the numbers of pan γδ T lymphocytes and studied their subtypes in the patients with active and inactive disease. These cell subpopulations were doubled in the treated patients with immunosuppressive agents in comparison with untreated ones; however, data were not statistically significant. Our study indicated that Vδ3+ subtype of γδ T cells seems to be involved in SLE pathogenesis; however, we accept the idea that the autoimmunity does not develop from a single abnormality, but rather from a number of different events.

scholarly journals 1040 Unexpected prevalence of subtle systolic and diastolic dysfunction in genotype-positive phenotype-negative relatives of dilated cardiomyopathy patients

2020 ◽  
Vol 21 (Supplement_1) ◽  
Author(s):  
A Paldino ◽  
G De Angelis ◽  
M Dal Ferro ◽  
G Faganello ◽  
A Porcari ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Diastolic Dysfunction ◽  
Longitudinal Strain ◽  
Global Longitudinal Strain ◽  
Prognostic Significance ◽  
Left Ventricular ◽  
Control Group ◽  
Healthy Individuals ◽  
Early Therapy ◽  
And Control

Abstract Funding Acknowledgements None Background Left ventricular global longitudinal strain (LV-GLS) and peak left atrial longitudinal strain (PALS) are promising techniques for the detection of a subtle systolic and diastolic dysfunction for an early diagnosis of genetically determined dilated cardiomyopathy (DCM). Purpose. We sought to evaluate the prevalence of subtle systolic and diastolic dysfunction by LV-GLS and PALS in a cohort of genotype-positive phenotype-negative (GPFN) DCM relatives. Methods. We analysed echocardiograms (including LV-GLS and PALS) of 41 GPFN relatives of DCM patients (GPFN group - 37 ± 14 years, 48.8% male). They were compared with a matched group of 52 healthy individuals (control group). Reduced LV-GLS and PALS were defined as &gt;-18% and &lt;23.1%, respectively, according to literature data. Results. GPFN and control groups were grossly similar according to standard echocardiographic measurements. Conversely, mean LV-GLS was -18.8 ± 2.7% in the GPFN group vs. -24.0 ± 1.8% in the control group (p &lt; 0.001). 20 subjects (48.8%) in the GPFN group and no subjects in the control group had a reduced LV-GLS. The mean PALS was 29.2 ± 6.7% in the GPFN group vs. 40.8 ± 8.5% in the control group (p &lt; 0.001). 7 subjects (18.4%) in the GPFN group and one (2%) in the control group had a reduced PALS. During a median follow-up of 27 months, 6 (14.6%) GPFN relatives developed a LV ejection fraction &lt;50%. Among them, 4 (66%) had a reduced LV-GLS at initial evaluation. Conclusions. LV-GLS and PALS are impaired in GPFN relatives of DCM patients compared to healthy individuals, when standard echocardiographic parameters are normal. Further studies are warranted to add prognostic significance to this result, which may lead in the future to an early therapy initiation. Abstract 1040 Figure. Mean LV-GLS and PALS in GPFN and control

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