Platelets in Storage: Altered S-Nitrosothiol Metabolism and Decreased Responsiveness May Be Related.

Abstract Under normal physiologic conditions, platelets circulate in an inactivated state and require only minimal stimulation ensuring their immediate availability for hemostasis. Previous research has shown that during platelet pheresis and storage, platelets undergo early activation resulting in expression of the surface activation marker CD62, release of α granule contents, and a decrease in inducible activation. In addition to regulating vascular tone, nitric oxide (NO) groups in plasma may provide tonic inhibition to circulating platelets thereby preventing the inappropriate adhesion and aggregation seen in stored platelets. S-Nitrosothiols (RSNOs) may serve as a reservoir for nitric oxide (NO) in the normal circulation. We hypothesize that platelets are prematurely activated during the process of apheresis in part due to the lack of RSNOs in the microenvironment during collection and storage. Furthermore, RSNO metabolism by stored platelets is changed by the process of apheresis itself. To test this hypothesis, we monitored the activation status of platelets using CD62 flow cytometry and quantified intracellular NO content and uptake using the fluorescence probe diaminodifluorofluorescein diacetate (DAF-FM DA). Both measurements were made on freshly isolated platelets (Fl-Plts) and on apheresis platelets (A-Plts). In addition, we employed these techniques to evaluate the responsiveness of Fl-Plts and A-Plts to agonists [thrombin receptor activating peptide (TRAP), ADP, and collagen] and to NO-donating molecules after varying storage times. In preliminary studies we have found that Fl-Plts bind RSNOs and demonstrate steroselectivity in their degree of uptake. Fl-Plts incubated with L-RSNOs produce over 2-fold the fluorescence compared to Fl-Plts incubated with D-RSNOs. A-Plts lose this stereoselectivity in RSNO uptake following the collection and storage process. We have also found that A-Plts have up to a 10-fold increase in uptake of nitric oxide from RSNOs compared to Fl-Plts. This significant increase and loss of stereoselectivity in RSNO uptake in A-Plts correlates with the loss of inducible activation over storage time. These data suggest RSNO metabolism in platelets is fundamentally altered during apheresis, collection, and storage and provides a novel target with therapeutic potential for further investigation into the loss of platelet function during storage.

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Komposisi Kimia dan Mikrobiologi Bumbu Instan “Soto Madura”

Journal of Food Technology and Agroindustry ◽

10.24929/jfta.v1i2.729 ◽

2019 ◽

Vol 1 (2) ◽

pp. 25-30

Author(s):

Ratih Yuniastri

Keyword(s):

Proximate Analysis ◽

Acid Number ◽

Nutritional Content ◽

Storage Process ◽

And Storage

Instant seasoning is made by mixing several ingredients and mashing it according to the desired composition. What is offered spices to enhance the flavor and aroma of these herbs. “Soto Madura” needs to be considered to determine the proper packaging and storage process. The spices are then made an analysis of the nutritional content of the spices. The spices were made repeatedly as many 3 times so the repeat data obtained. The analysis carried out includes proximate analysis, analysis of fat damage, and microbilogical content of herbs. The results of the analysis showed the instant seasoning “Soto Madura” contained water of 7,42±0,066%; ash by 1,18±0,056%; protein of 59,04±0,085%; fat 7,77±0,152%; carbohydrates 25,46±0,531%; acid number 0,507±0,035 mg/g; peroxide rate 4,063±0,066 Meg/1000g; free fat (FFA) 0,22±0,036%. For microbiological content, the content of microorganisms in the spices was 1,11x108 colonies; mold 7,9x106 colonies; and coliforn 8,6x106 colonies.

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Therapeutic Potential of Phosphodiesterase Inhibitors for Endothelial Dysfunction- Related Diseases

Current Pharmaceutical Design ◽

10.2174/1381612826666200403172736 ◽

2020 ◽

Vol 26 (30) ◽

pp. 3633-3651 ◽

Cited By ~ 2

Author(s):

Javier Blanco-Rivero ◽

Fabiano E. Xavier

Keyword(s):

Nitric Oxide ◽

Endothelial Dysfunction ◽

Endothelial Function ◽

Therapeutic Potential ◽

Treatment Strategies ◽

Phosphodiesterase Inhibitors ◽

Developed Countries ◽

Major Health Problem ◽

Pde Inhibitors ◽

Pharmaceutical Industries

Cardiovascular diseases (CVD) are considered a major health problem worldwide, being the main cause of mortality in developing and developed countries. Endothelial dysfunction, characterized by a decline in nitric oxide production and/or bioavailability, increased oxidative stress, decreased prostacyclin levels, and a reduction of endothelium-derived hyperpolarizing factor is considered an important prognostic indicator of various CVD. Changes in cyclic nucleotides production and/ or signalling, such as guanosine 3', 5'-monophosphate (cGMP) and adenosine 3', 5'-monophosphate (cAMP), also accompany many vascular disorders that course with altered endothelial function. Phosphodiesterases (PDE) are metallophosphohydrolases that catalyse cAMP and cGMP hydrolysis, thereby terminating the cyclic nucleotide-dependent signalling. The development of drugs that selectively block the activity of specific PDE families remains of great interest to the research, clinical and pharmaceutical industries. In the present review, we will discuss the effects of PDE inhibitors on CVD related to altered endothelial function, such as atherosclerosis, diabetes mellitus, arterial hypertension, stroke, aging and cirrhosis. Multiple evidences suggest that PDEs inhibition represents an attractive medical approach for the treatment of endothelial dysfunction-related diseases. Selective PDE inhibitors, especially PDE3 and PDE5 inhibitors are proposed to increase vascular NO levels by increasing antioxidant status or endothelial nitric oxide synthase expression and activation and to improve the morphological architecture of the endothelial surface. Thereby, selective PDE inhibitors can improve the endothelial function in various CVD, increasing the evidence that these drugs are potential treatment strategies for vascular dysfunction and reinforcing their potential role as an adjuvant in the pharmacotherapy of CVD.

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Use of Families of Euphorbiaceae for the Production of Biodiesel in Semiarid Areas

Proceedings ◽

10.3390/proceedings2020052002 ◽

2021 ◽

Vol 52 (1) ◽

pp. 2

Author(s):

Noé Anes García ◽

Antonio Luis Marqués Sierra

Keyword(s):

Fossil Fuels ◽

Chemical Properties ◽

Petroleum Products ◽

Prolonged Storage ◽

Physical Chemical ◽

Semiarid Areas ◽

Storage Times ◽

And Storage ◽

Improved Characteristics ◽

Made In

In recent years, developments made to reduce the consequences generated using petroleum products have been strengthening; therefore, biofuels have become a requirement in different countries worldwide with the objective of reducing not only the high levels of current pollution, but also mitigating the effects generated by global warming. Despite the advances that have been made in the field of research on Jatropha, it is still necessary to carry out more detailed studies aimed at achieving a better use of it, identifying the influence of its physical–chemical properties in terms of quality levels, as well as determining its behavior when mixed with palm oil to achieve a biodiesel with better yields, whose impact will be reflected mainly in the environmental field, helping to mitigate the production of greenhouse gases that are produced by petroleum products. Although currently the biofuels sector has made important advances in research, it is necessary to deepen the physical–chemical analyses both in the production and storage processes of biodiesel, so that in the future it can be fully fulfilled with the energy requirements that are currently only achieved with fossil fuels, so it is necessary to direct this research toward the development of new products with improved characteristics, especially when exposed to prolonged storage times and low temperatures.

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The Actin-Sequestering Protein Thymosin Beta-4 Is a Novel Target of Hypoxia-Inducible Nitric Oxide and HIF-1α Regulation

PLoS ONE ◽

10.1371/journal.pone.0106532 ◽

2014 ◽

Vol 9 (10) ◽

pp. e106532 ◽

Cited By ~ 1

Author(s):

Yun-Kyoung Ryu ◽

Joo-Hyun Kang ◽

Eun-Yi Moon

Keyword(s):

Nitric Oxide ◽

Thymosin Beta 4 ◽

Novel Target ◽

Inducible Nitric Oxide

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Transit and storage times of bipolar transistors in a VLSI environment

IEE Proceedings I Solid State and Electron Devices ◽

10.1049/ip-i-1.1983.0027 ◽

1983 ◽

Vol 130 (3) ◽

pp. 151

Author(s):

S.S. Rofail

Keyword(s):

Bipolar Transistors ◽

Storage Times ◽

And Storage

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The Effect of Hydrothermal Treatment on Metabolite Composition of Hass Avocados Stored in a Controlled Atmosphere

Plants ◽

10.3390/plants10112427 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2427

Author(s):

Rosana Chirinos ◽

David Campos ◽

Sofía Martínez ◽

Sílfida Llanos ◽

Indira Betalleluz-Pallardel ◽

...

Keyword(s):

Hydrothermal Treatment ◽

Nutritional Value ◽

Syringic Acid ◽

Controlled Atmosphere ◽

Coumaric Acid ◽

Primary And Secondary Metabolites ◽

Avocado Fruit ◽

Lipophilic Antioxidant ◽

Storage Times ◽

And Storage

Avocado cv. Hass consumption has expanded worldwide given its nutritional, sensory, and functional attributes. In this work, avocado fruit from two harvests was subjected to hydrothermal treatment (38 °C for 1 h) or left untreated (control) and then stored for 30 and 50 days in a controlled atmosphere (4 kPa O2 and 6 kPa CO2 at 7 °C) (HTCA and CA, respectively) with subsequent ripening at ~20 °C. The fruit was evaluated for primary and secondary metabolites at harvest, after storage, and after reaching edible ripeness. A decrease from harvest to edible ripeness in mannoheptulose and perseitol was observed while β-sitosterol, hydrophilic and lipophilic antioxidant activity (H-AOX, L-AOX), abscisic acid, and total phenolics (composed of p-coumaric and caffeic acids such as aglycones or their derivatives) increased. HTCA fruit at edible ripeness displayed higher contents of mannoheptulose, perseitol, β-sitosterol, L-AOX, caffeic acid, and p-coumaric acid derivatives, while CA fruit presented higher contents of α-tocopherol, H-AOX, and syringic acid glycoside for both harvests and storage times. The results indicate that a hydrothermal treatment prior to CA enables fruit of high nutritional value characterized by enhanced content of phenolic compounds at edible ripeness to reach distant markets.

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Karakteristik Fisikokimia dan Aktifitas Antioksidan Daging Itik yang Dilapisi Ekstrak Propolis Selama Penyimpanan Suhu Ruang

Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan ◽

10.29244/jipthp.9.3.151-157 ◽

2021 ◽

Vol 9 (3) ◽

pp. 151-157

Author(s):

A. Apriantini ◽

S. Adinata ◽

Y. C. Endrawati

Keyword(s):

Shelf Life ◽

Storage Time ◽

Ph Value ◽

Edible Coating ◽

Cooking Loss ◽

Propolis Extract ◽

Randomized Design ◽

Completely Randomized Design ◽

Storage Times ◽

And Storage

Duck meat is a perishable food that has a short shelf-life. Therefore, packaging is needed to be used tomaintain duck meat quality during storage, one of the ways to extend shelf-life of duck meat is usingpropolis extract as edible coating which is contained antioxidant substances which can be used to extendduring storage. The aim of this research was to analyze the effect of propolis extract as an edible coatingmaterial on duck meat to maintain the quality and extend shelf-life of duck meat at room temperature.The research was used a completely randomized design (CRD) factorial 4x3 with 3 replications,consisting of 4 propolis concentrations (0.0%, 0.5%, 1.0%, 1.5%) and 3 storage times (0 hours, 6 hoursand 24 hours). Duck meats coated with propolis extracts had a significant effect on the water content andantioxidant activity of duck meat (P < 0.05). The storage time had a significant effect on the pH valueand the cooking loss of duck meat (P <0.05). The addition of propolis extract and storage time had asignificant effect on the pH value, cooking loss and antioxidant activity of duck meat (P <0.05).

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THE IMPLEMENTATION OF INDIGENOUS KNOWLEDGE IN PRESERVING UNIVERSITAS INDONESIA LIBRARY’S MANUSCRIPTS

International Review of Humanities Studies ◽

10.7454/irhs.v4i1.150 ◽

2019 ◽

Vol 4 (1) ◽

Author(s):

Deka Anggawira ◽

Tamara Adriani Salim

Keyword(s):

Data Collection ◽

Indigenous Knowledge ◽

Direct Observation ◽

Qualitative Approach ◽

Storage Process ◽

Structured Interviews ◽

Traditional Approaches ◽

Maintenance Process ◽

And Storage ◽

Collection Methods

This study discusses the implementation of local wisdom in the preservation of manuscripts at Universitas Indonesia’s library. The purpose of this study is to identify the implementation of local wisdom in the preservation of manuscripts in that library. This research uses a qualitative approach coupled with direct observation and structured interviews as data collection methods. The results of this study indicate that Universitas Indonesia Library has implemented local wisdom in preserving manuscripts. This can be seen from the use of local wisdom in the storage process, including the design of the rooms and storage facilities and the pattern of behavior in its storage process. The maintenance process of local wisdom includes the control of the environment using traditional approaches and the use of traditional materials in the maintenance of manuscripts. Another finding is that the process of capturing or inheriting knowledge from a previous manuscript is based on the manpower manifested in its preservation behavior. Therefore, it can be understood that the implementation of local wisdom in the process of preservation of manuscripts in UI Library is based on the preservation of knowledge from previous manuscript managers or librarians.

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