LncRNA NEAT1 is upregulated in recurrent aphthous stomatitis (RAS) and has predictive values

Abstract Background LncRNA NEAT1 promotes inflammatory responses, which contribute to recurrent aphthous stomatitis (RAS). This study focused on the involvement of NEAT1 in RAS. Methods RT-qPCR and ELISA were performed to determine the expression of NEAT1 and proinflammatory factors (IL-2, IL-1β, and TNF-α) in plasma from patients with a history of RAS and showing symptom (n = 80, S-RAS group), people with a history of RAS but showing no symptom (n = 80, NS-RAS group), and controls without a history of RAS (n = 80, Control group). Correlation analysis was performed with Pearson’s correlation coefficient. S-RAS group received treatmen,t and plasma levels of NEAT1 and proinflammatory factors were compared before and after treatment. S-RAS group was followed up for 12 months, and the recurrence was recorded. Results Plasma NEAT1, IL-2, IL-1β, and TNF-α levels were the highest in the S-RAS group, followed in turn by NS-RAS and control groups. NEAT1 was positively and significantly correlated with IL-2, IL-1β, and TNF-α across S-RAS and NS-RAS samples, but not control samples. After treatment, plasma levels of NEAT1, IL-2, IL-1β, and TNF-α decreased significantly. Moreover, a higher recurrence rate was observed during the follow-up in patients with high plasma NEAT1 levels. Conclusion NEAT1 is upregulated in RAS and correlated with multiple proinflammatory factors. Moreover, NEAT1 has predictive values for RAS.

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The Impact of DNA Methylation on IL6 mRNA Levels in Hematinic Deficiency and Atopy-Associated Recurrent Aphthous Stomatitis Patients

International Journal of Dentistry ◽

10.1155/2021/5560695 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Nanan Nur’aeny ◽

Dida Akhmad Gurnida ◽

Oki Suwarsa ◽

Irna Sufiawati

Keyword(s):

Dna Methylation ◽

Recurrent Aphthous Stomatitis ◽

Aphthous Stomatitis ◽

Control Group ◽

Mrna Levels ◽

Modified Dna ◽

Increased Risk ◽

History Of ◽

The Impact ◽

Il6 Gene

Objective. To investigate the DNA methylation using pyrosequencing and its effects on the upregulation of IL6 mRNA in patients with recurrent aphthous stomatitis (RAS) in connection with hematinic deficiency and atopy. Material and Methods. This cross-sectional study was conducted at Dr. Hasan Sadikin Hospital, Bandung, from January–March 2019 and was approved by the Health Research Ethics Committee of Universitas Padjadjaran (Ethics No. 990/UN6.KEP/EC/2018). Furthermore, the subjects had RAS ulcers with a history of at least twice a year along with atopy and dietary imbalance with no history of recurrent intraoral herpes or any systemic diseases. This study was performed on 23 RAS patients and 21 healthy subjects, and the sampling was carried out consecutively. The blood samples were collected from all the subjects, and then, the DNA and RNA were extracted from the peripheral blood mononuclear cells (PBMCs). Consequently, the bisulfite-modified DNA was used to confirm the methylation status of the IL6 gene promoter through the pyrosequencing method. The methylation levels of the IL6 promoter were assessed by a reverse transcriptase-polymerase chain reaction technique. The gene expression of RAS and the control group was analyzed by the 2−ΔΔCT method. The statistical analysis using the Mann–Whitney U test was conducted to evaluate IL6 mRNA levels and DNA methylation with p value <0.05 considered to be statistically significant. Result. The IL6 mRNA levels were approximately 1.88-fold in RAS patients, and there was a significant relationship between the expression of the IL6 gene and the increased risk of RAS p < 0.001 . It was reported that four out of six sites in the cytosine phosphate guanine (CpG) island IL6 promoter had a lower degree of methylation, and two other sites in patients with RAS had greater methylation compared with control, but not statistically significant. Conclusion. This study showed the upregulation of IL6 mRNA levels in RAS patients compared to control. DNA methylation in the present study is at sites 566–658, whereas the location of the IL6 promoter is at sites 1–1684. Thus, it would be necessary conducting some research at other CpG sites of IL6 promoter islands to determine the status of DNA methylation.

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Insulin Resistance and Inflammation in Black Women with and without Breast Cancer: Cause for Concern

Ethnicity & Disease ◽

10.18865/ed.26.4.513 ◽

2016 ◽

Vol 26 (4) ◽

pp. 513 ◽

Cited By ~ 2

Author(s):

Kathleen A. Griffith ◽

Seon Yoon Chung ◽

Shijun Zhu ◽

Alice S. Ryan

Keyword(s):

Breast Cancer ◽

Insulin Resistance ◽

Black Women ◽

White Women ◽

Cancer Recurrence ◽

Control Group ◽

Study Objective ◽

Increased Risk ◽

Tnf Α ◽

History Of

Objective: After chemotherapy for breast cancer, Black women gain more weight and have an increased mortality rate compared with White women. Our study objective was to compare biomarkers associated with obesity in Black women with and without a history of breast cancer.Design: Case-controlSetting: Academic/federal institutionParticipants: Black women with a history of breast cancer (cases) and age-matched controls.Methods: We compared insulin resistance, inflammation, and lipids in overweight and obese Black women with a history of breast cancer (n=19), age similar controls (n=25), and older controls (n=32). Groups did not differ on mean body mass index (BMI), which was 35.4 kg/m2, 36.0 kg/m2, and 33.0 kg/m2, respectively.Main Outcome Measures: Insulin resistance (HOMA-IR); inflammation (TNF-α, IL-1b, IL-6, IL-8, CRP); lipids (cholesterol, triglycerides).Results: Cases had 1.6 and 1.38 times higher HOMA-IR values compared with age similar and older controls, respectively (P≤.001 for both). TNF-α and IL-1b were significantly higher in cases compared with both control groups (P<.001 for both). IL-6 was also higher in cases compared with age-similar controls (P=.007), and IL-8 was lower in cases compared with older controls (P<.05). Lipids did not differ between cases and either control group.Conclusions: Black women with breast cancer were significantly more insulin resistant with increased inflammation compared not only with age similar controls but with women who were, on average, a decade older. These biomarkers of insulin resistance and inflammation may be associated with increased risk of breast cancer recurrence and require ongoing evaluation, especially given the relatively abnormal findings compared with the controls in this underserved group. Ethn Dis. 2016;26(4):513-520; doi:10.18865/ed.26.4.513

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RECURRENT APHTHOUS STOMATITIS RELATED TO PSYCHOLOGICAL STRESS, FOOD ALLERGY AND GERD

ODONTO Dental Journal ◽

10.30659/odj.6.0.45-51 ◽

2019 ◽

Vol 6 ◽

pp. 45

Author(s):

Rina Kartika Sari ◽

Diah Savitri Ernawati ◽

Bagus Soebadi

Keyword(s):

Food Allergy ◽

Psychological Stress ◽

Oral Mucosa ◽

Recurrent Aphthous Stomatitis ◽

Predisposing Factors ◽

Aphthous Stomatitis ◽

Cow Milk ◽

Esophageal Mucosa ◽

History Of ◽

Multiple Ulcers

Background: Recurrent Aphthous Stomatitis (RAS) is inflammation in oral mucosa characterized by recurrent single or multiple ulcers that usually affected in non keratinized mucosa. Etiology RAS is unknown but psychological stress, allergy, and gastrointestinal disease can be predisposing factors Case Management: A 23rd years old complained recurrent oral ulcer with free ulcer period for 3-5 days. The patient had a history of food allergy, GERD and psychological stress. Intraoral examination showed recurrent multiple ulcers in variation site of the mouth. DASS 42 screening showed high stress and high anxiety. Skin Prick Test showed positive allergy to kapok, beef, chicken, cow milk, white egg, duck egg, shrimp, cob fish, milkfish, chocolate, and peanut. Ulcers treated with nonsteroid antiinflammation Aloe Vera gel and stress management by reading assignment method.Discussion: Psychological stress altered the immune system so oral mucosa prone to inflammation, and make the history of GERD getting worse. Stress causes cortisol secretion that changes the imbalance of proinflammatory and antiinflammatory cytokines. Oral mucosa becomes more susceptible to hypersensitivity. In addition, stress decreased oral and esophageal mucosa resistance to GERDConclusion: RAS triggered by psychological stress, allergy, and GERD. Treatment of RAS is by elimination predisposing factors to prevent recurrence.

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Depression, comorbidities and the TNF-α system

European Psychiatry ◽

10.1016/j.eurpsy.2008.03.013 ◽

2008 ◽

Vol 23 (6) ◽

pp. 421-429 ◽

Cited By ~ 111

Author(s):

H. Himmerich ◽

S. Fulda ◽

J. Linseisen ◽

H. Seiler ◽

G. Wolfram ◽

...

Keyword(s):

Plasma Levels ◽

Smoking Status ◽

Normal Population ◽

Max Planck ◽

Nutrition Survey ◽

Representative Study ◽

Tnf Α ◽

History Of ◽

Soluble Tnf Receptor ◽

History Of Depression

AbstractDepression has frequently been reported to be associated with other physical diseases and changes in the cytokine system. We aimed to investigate associations between a medical history of depression, its comorbidities and cytokine plasma levels in the Bavarian Nutrition Survey II (BVS II) study sample and in patients suffering from an acute depressive episode.The BVS II is a representative study of the Bavarian population aged 13–80 years. The disease history of its 1050 participants was assessed through face-to-face interviews. A sub-sample of 568 subjects and 62 additional acutely depressed inpatients of the Max Planck Institute of Psychiatry participated in anthropometric measurements and blood sampling. Tumor necrosis factor-α (TNF-α) and soluble TNF receptor (sTNF-R) p55 and sTNF-R p75 plasma levels were measured using enzyme-linked immunosorbent assays.A history of depression was associated with a higher incidence of high blood pressure, peptic ulcer, dyslipoproteinemia, osteoporosis, allergic skin rash, atopic eczema and thyroid disease.Within the BVS II sample, participants with a history of depression differed from subjects who had never had depression with regard to sTNF-R p55 and sTNF-R p75 levels even when controlling for age, BMI and smoking status. Acutely depressed inpatients showed even higher levels of sTNF-R p55 and sTNF-R p75 than subjects in the normal population. TNF-α levels were also significantly elevated in acutely depressed patients.These results confirm earlier studies regarding the comorbidities of depression and support the hypothesis that activation of the TNF-α system may contribute to the development of a depressive disorder.

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Mannose-binding lectin serum levels and (Gly54asp) gene polymorphism in recurrent aphthous stomatitis: A case-control study

International Journal of Immunopathology and Pharmacology ◽

10.1177/20587384211064454 ◽

2021 ◽

Vol 35 ◽

pp. 205873842110644

Author(s):

Shereen A Baioumy ◽

Shaimaa H Fouad ◽

Shaimaa A Abdalgeleel ◽

Ahmed A Baiomy ◽

Dina E Sallam ◽

...

Keyword(s):

Case Control Study ◽

Serum Levels ◽

Case Control ◽

Recurrent Aphthous Stomatitis ◽

Mannose Binding Lectin ◽

Aphthous Stomatitis ◽

Control Group ◽

Mannose Binding ◽

Binding Lectin ◽

Control Study

Objectives: Dysregulation of the immune response appears to play a significant role in recurrent aphthous stomatitis (RAS) development. The main objective of this case–control study is to investigate the blood levels of mannose-binding lectin (MBL) and the frequency of the MBL2 gene (gly54asp) polymorphism in RAS patients, including 40 RAS patients and 40 healthy controls. Methods: Serum MBL levels were determined by ELISA, while the PCR-restriction fragment length polymorphism was used in MBL2 genotyping. Results: The median serum MBL level was significantly lower in the RAS group than in the control group (975 ng/mL (545–1320) vs. 1760 ng/mL (1254–2134); p≤ 0.001). The MBL levels were significantly lower in the BB genotype, whereas they were significantly higher in the wild type AA with a median of 525 and 1340 ng/mL, respectively ( p =0.005). The B allele was expressed in significantly higher percentages of RAS patients than in controls. There was no significant association between MBL serum levels ( p=0.685) or MBL2 codon 54 genotypes ( p=0.382) with the type of ulcers. Conclusion: There was an association between low MBL serum levels and the variant allele B of the MBL2 (gly54asp) gene, and the susceptibility to RAS. As a result, potential novel therapeutic options for RAS patients with MBL deficiency should be investigated.

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Puerarin mitigates acute liver injury in septic rats by regulating proinflammatory factors and oxidative stress levels

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v20i11.11 ◽

2021 ◽

Vol 20 (11) ◽

pp. 2305-2310

Author(s):

Jinan Zheng ◽

Qing Huang ◽

Jingjing Fang

Keyword(s):

Oxidative Stress ◽

Liver Injury ◽

Acute Liver Injury ◽

Serum Levels ◽

Expression Level ◽

Sepsis Group ◽

Control Group ◽

Sprague Dawley ◽

Tnf Α ◽

Proinflammatory Factors

Purpose: To determine the protective effect of puerarin against acute liver injury in septic rats, and the mechanism involved.Methods: Eighty-seven Sprague-Dawley (SD) rats were assigned to control, sepsis and puerarin groups (each having 29 rats). Serum levels of NF-kB, TNF-α, IL-1 β, IL-6, ALT and AST were assayed. Liver lesions and levels of NO, SOD, iNOS and malondialdehyde (MDA) were measured using standard procedures.Results: Compared with the control group, the levels of NF-kB, TNF-α, IL-1β, IL-6, AST, ALT, NO, MDA and iNOS significantly increased in the sepsis group, while SOD level decreased significantly. In contrast, there were marked decreases in NF-kB, TNF-α, IL-1β, AST, ALT, NO, MDA and iNOS in puerarin group, relative to the sepsis group, while SOD expression level was significantly increased (p <0.05). The level of p-p38 in liver of septic rats was up-regulated, relative to control rats, while Nrf2 significantly decreased (p < 0.05). The expression level of p-p38 in the puerarin group was significantly decreased, relative to the sepsis group, while the expression level of Nrf2 significantly increased (p < 0.05).Conclusion: Puerarin mitigates acute liver injury in septic rats by inhibiting NF-kB and p38 signaling pathway, down-regulating proinflammatory factors, and suppressing oxidative stress. Thus, puerarin may be developed for use in the treatment liver injury.

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Evaluation of Salivary Interferon-Gamma Level in Patients with Recurrent Aphthous Stomatitis

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v28i9.4773 ◽

2020 ◽

Author(s):

Marieh Honarmand ◽

Ramin Saravani ◽

Hossein Ansari ◽

Iman Teimoori

Keyword(s):

Interferon Gamma ◽

Systemic Disease ◽

Recovery Period ◽

Recurrent Aphthous Stomatitis ◽

Aphthous Stomatitis ◽

Control Group ◽

Healthy Individuals ◽

Salivary Level ◽

The Mean ◽

Control Study

Introduction: Recurrent aphthous stomatitis (RAS) is defined by recurring ulcers restricted to oral mucosa in the patients with no other signs of systemic disease. The purpose of this study was to evaluate the salivary level of interferon gamma in patients with RAS. Methods: In this case-control study, 30 patients with RAS (in Recurrence and recovery period) in the experimental and 25 healthy people as the control group were compared. Salivary interferon gamma level was evaluated by the use of ELISA method. Data were analyzed via SPSS version 16 software with Mann-U-Whitney test and Wilcoxon. P <0.05 was considered significant. Results: The mean salivary interferon gamma levels were 45.01±31.02 pg/ml and 32.04±19.02 pg/ml in the patients with RAS and healthy individuals, respectively. (P>0.001). In addition, interferon gamma level was 45.01± 31.02 pg/ml and 58.03± 52.04 pg/ml in the recurrence and recovery periods, respectively. (P=0.67). Conclusion: Salivary interferon gamma levels were higher in the patients with RAS than healthy individuals.

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Protective effects of emodin on lung injuries in rat models of liver fibrosis

Open Life Sciences ◽

10.1515/biol-2019-0069 ◽

2019 ◽

Vol 14 (1) ◽

pp. 611-618

Author(s):

Lili Zhang ◽

Huiying Zhang ◽

Limin Wang ◽

Yimin Fan ◽

Cuiying Zhang ◽

...

Keyword(s):

Liver Fibrosis ◽

Inflammatory Responses ◽

Control Group ◽

Protective Effects ◽

Model Group ◽

Lung Weight ◽

Rat Models ◽

Tnf Α ◽

Lung Injuries ◽

Weight Coefficients

AbstractObjectiveThe aim of this study is to investigate the protective effects of emodin (EMD) on the lung injuries in the rat models of liver fibrosis.MethodsLiver fibrosis was established in rats and the effect of intervention using EMD treatment was determined. Liver and lung weight coefficients were measured and lung content of TNF-α (tumor necrosis factor α), MDA (malondialdehyde), NO (nitric oxide), and ONOO- (peroxynitrite) were determined. Finally, histopathological changes were evaluated.ResultsCompared with the normal control group, the lung weight coefficient was significantly increased in the fibrosis model group. Moreover, pulmonary edema and inflammatory responses were observed. Levels of TNF-α, MDA, NO, and ONOO- in the lung homogenate were significantly increased in the fibrosis model group. After EMD treatment, the lung weight coefficients were significantly reduced. Moreover, pathological changes in the lung tissue were dramatically alleviated. Levels of TNF-α, MDA, NO, and ONOO- were significantly decreased.ConclusionEMD exhibits protective effects against lung injuries in a rat model of liver fibrosis.

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Clinical Evaluation of 660 nm Diode Laser Therapy on the Pain, Size and Functional Disorders of Recurrent Aphthous Stomatitis

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.268 ◽

2019 ◽

Vol 7 (9) ◽

pp. 1516-1522 ◽

Cited By ~ 1

Author(s):

Hisham Abdelmonem Soliman ◽

Diana Mostafa

Keyword(s):

Laser Therapy ◽

Diode Laser ◽

Pathological Condition ◽

Ventral Surface ◽

Healing Time ◽

Recurrent Aphthous Stomatitis ◽

Aphthous Stomatitis ◽

Control Group ◽

Functional Disorders ◽

Significant Difference

BACKGROUND: Minor recurrent aphthous stomatitis (MiRAS) is one of the most common, recurrent, and painful mucosal pathological condition. It is characterised by round or shallow oval ulcers, less than 10 mm in diameter, surrounded by a thin erythematous halo. It involves non-keratinized mucosa such as the labial and buccal mucosa, the ventral surface or borders of the tongue and the floor of the mouth, but it is uncommon to occur on the keratinised mucosa. It heals spontaneously within 10-14 days without scarring. There is no curative remedy to prevent its recurrence; also, available modalities only reduce the symptoms and severity of the lesion. AIM: Since these lesions may be extremely painful, we decided to estimate the pain-relieving and healing properties of low energy level laser therapy using diode laser 660 nm on MiRAS. MATERIAL AND METHODS: Twenty healthy patients suffering from minor aphthous ulcers were randomly selected from the Out-Patient Clinic of Oral Medicine Department, Faculty of Dentistry, Alexandria University. They were equally divided into two groups, study group who received 660 nm diode laser irradiation while the control group received placebo (sodium bicarbonate rinse). The visual analogue scale, size reduction, effectiveness indices and functional disorders were compared between the groups. RESULTS: Both groups presented a statistically significant difference from baseline to follow up periods. But, diode laser 660 nm treatment showed more remarkable improvements in reduction of healing time, pain and lesion size. CONCLUSION: We concluded that diode laser 660 nm should be further considered as an effective alternative therapeutic regimen to patients who suffer from recurrent aphthous stomatitis.

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Exosomal neurofilament light

Neurology ◽

10.1212/wnl.0000000000009577 ◽

2020 ◽

Vol 94 (23) ◽

pp. e2412-e2423 ◽

Cited By ~ 5

Author(s):

Vivian A. Guedes ◽

Kimbra Kenney ◽

Pashtun Shahim ◽

Bao-Xi Qu ◽

Chen Lai ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Plasma Levels ◽

Depression Symptoms ◽

Neurofilament Light ◽

Tnf Α ◽

Ultrasensitive Assay ◽

Postconcussive Syndrome ◽

History Of ◽

Endothelial Growth Factor

ObjectiveTo measure exosomal and plasma levels of candidate blood biomarkers in veterans with history of mild traumatic brain injury (mTBI) and test their relationship with chronic symptoms.MethodsExosomal and plasma levels of neurofilament light (NfL) chain, tumor necrosis factor (TNF)–α, interleukin (IL)–6, IL-10, and vascular endothelial growth factor (VEGF) were measured using an ultrasensitive assay in a cohort of 195 veterans, enrolled in the Chronic Effects of Neurotrauma Consortium Longitudinal Study. We examined relationships between candidate biomarkers and symptoms of postconcussive syndrome (PCS), posttraumatic stress disorder (PTSD), and depression. Biomarker levels were compared among those with no traumatic brain injury (TBI) (controls), 1–2 mTBIs, and repetitive (3 or more) mTBIs.ResultsElevated exosomal and plasma levels of NfL were associated with repetitive mTBIs and with chronic PCS, PTSD, and depression symptoms. Plasma TNF-α levels correlated with PCS and PTSD symptoms. The total number of mTBIs correlated with exosomal and plasma NfL levels and plasma IL-6. Increased number of years since the most recent TBI correlated with higher exosomal NfL and lower plasma IL-6 levels, while increased number of years since first TBI correlated with higher levels of exosomal and plasma NfL, as well as plasma TNF-α and VEGF.ConclusionRepetitive mTBIs are associated with elevated exosomal and plasma levels of NfL, even years following these injuries, with the greatest elevations in those with chronic PCS, PTSD, and depression symptoms. Our results suggest a possible neuroinflammatory and axonal disruptive basis for symptoms that persist years after mTBI, especially repetitive.

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