Antiproliferative, antibacterial, and antioxidant activities of Bauhinia strychnifolia Craib aqueous extracts in gut and liver perspective

Abstract Background Bauhinia strychnifolia Craib is an herb in Thai traditional medicine. Its decoction is traditionally used as an anticancer, antidiarrheal, and hangover remedy for centuries. Several studies described bioactivities of its organic solvent extracts, however, only few demonstrated the usefulness of the decoction. Here, we aimed to determine the bioactivities of Bauhinia strychnifolia Craib root and stem aqueous extracts in gut and liver perspective. Methods To achieve the goal, we performed MTT test, microscopic analyses, disc diffusion assay, broth microdilution assay, free radicals scavenging assays, and LC-MS analysis. Results We found that the extracts inhibited the growth of human hepatocellular carcinoma (HepG2) and colon adenocarcinoma (HT-29) cell lines. Moreover, they also inhibited the growth of gram-positive bacteria Staphylococcus aureus and Bacillus cereus but not inhibited the growth of gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Furthermore, the extracts exhibited moderate antioxidant activity and increased GSH production in HepG2 cell line when compared with untreated. Our LC-MS analysis confirmed the existence of anticancer and antioxidant; 3,5,7,3′,5′-pentahydroxyflavanonol-3-O-α-L-rhamnopyranoside and β-sitosterol, in the extracts. Conclusion The results from our study supported that the administration of Bauhinia strychnifolia Craib root and stem decoction would really aid colon or liver cancer patients and detoxify the alcoholic drunkard as it is claimed in Thai traditional medicine.

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Enaminone-Derived Pyrazoles with Antimicrobial Activity

Journal of Chemistry ◽

10.1155/2019/2467970 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10

Author(s):

Mashooq Ahmad Bhat ◽

Mohamed A. Al-Omar ◽

Ahmed M. Naglah ◽

Abdul Arif Khan

Keyword(s):

Antimicrobial Activity ◽

Gram Negative Bacteria ◽

Significant Activity ◽

Disc Diffusion ◽

Initial Screening ◽

Gram Positive ◽

Gram Negative ◽

Disc Diffusion Assay ◽

Gram Positive Bacteria ◽

Diffusion Assay

A series of pyrazoles derived from the substituted enaminones were synthesized and were evaluated for antimicrobial activity. All the compounds were characterized by the spectral data and elemental analysis. The synthesized compounds were initially screened for their antimicrobial activity against ATCC 6538, NCTC 10400, NCTC 10418, and ATCC 27853. During initial screening, compounds (P1, P6, and P11) presented significant antimicrobial activity through disc diffusion assay. These compounds were further evaluated for antimicrobial activity at different time points against Gram-positive and Gram-negative bacteria and presented significant activity for 6 hours. The activity was found to be greater against Gram-positive bacteria. In contrast at 24 hours, the activity was found only against Gram-positive bacteria except compound (P11), showing activity against both types of bacteria. Compound (P11) was found to have highest activity against both Gram-positive and Gram-negative bacteria.

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Chemical Composition, Antimicrobial, Cytotoxic and Antiplasmodial Activities of Three Sponges from Buton Islands, Indonesia

ILMU KELAUTAN Indonesian Journal of Marine Sciences ◽

10.14710/ik.ijms.22.3.147-154 ◽

2017 ◽

Vol 22 (3) ◽

pp. 147

Author(s):

Masteria Yunovilsa Putra ◽

Tri Aryono Hadi

Keyword(s):

Fungal Pathogen ◽

Brine Shrimp ◽

Antimicrobial Activities ◽

Marine Sponges ◽

Sponge Species ◽

Gram Negative Bacteria ◽

Significant Activity ◽

Gram Positive Bacteria ◽

Ms Analysis ◽

Inhibition Zones

GC-MS analysis of the crude extracts of three different species of Indonesian marine sponges has been carried out for identification of bioactive compounds. The GC-MS analysis from Haliclona (Gellius) sp., Lamellodysidea herbacea, and Spheciospongia inconstans revealed the presence of 23, 21, 19 various compounds, respectively and mainly sterols and fatty acids. All the sponge species has been evaluated for antimicrobial activities, cytotoxicity using brine shrimp lethality bioassay and heme polymerization inhibitory activity assay for antiplasmodial activity. In this study, all the sponge species showed antimicrobial activities against at leastone of the test strains. Among them, the extract of sponge Lamellodysidea herbacea displayed activity against two Gram-positive bacteria (S. aureus and B. subtilis) and the Gram-negative bacteria V. cholerae, with inhibition zones of 10.3, 9.2 and 9.5 mm, respectively. The sponge Haliclona (Gellius) sp., showed significant activity against fungal pathogen C. albicans. The sponge Haliclona (Gellius) sp., displayed the ability to inhibit heme polymerization indicating an anti-Plasmodium function and also showed potent cytotoxic activity against the brine shrimp Artemia sp. Keywords: GC-MS analysis, antimicrobial, sponges,bioactive

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Analysis of Phytoconstituents and Biological Activities on The Selected Medicinal Plants of Dolakha and Sindhualchowk District of Nepal

Journal of Institute of Science and Technology ◽

10.3126/jist.v22i2.19605 ◽

2018 ◽

Vol 22 (2) ◽

pp. 140-147 ◽

Cited By ~ 1

Author(s):

Bimala Subba ◽

Susila Thapa

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Antioxidant Power ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Pharmacologically Active

Phytochemical and biological activities of methanolic extract of seven plants viz. Scindapsus officinalis Schott. , Lepisorus loriformis, Nicotiana tabacum L., Clematis buchananiana DC., Astilbe rivularis D. Don, Potentilla fulgens Wall. ex Hook and Taxus wallichiana Zucc. were carried out. The brine shrimp bioassay showed that N. tabacum, A. rivularis, and P. fulgens were pharmacologically active. The antibacterial potential was studied against Staphylococcus aureus (gram positive bacteria) and Escherichia coli (gram negative bacteria) using Agar Well Diffusion Method. Roots of P. fulgens showed inhibition against gram positive bacteria while the rhizome of A. rivularis showed inhibition against both gram positive and gram negative bacteria. Antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power FRAP assay. Both assays showed that P. fulgens has high antioxidant activities with (IC50 =15.57 ± 3.6) lower than standard ascorbic acid. Journal of Institute of Science and TechnologyVolume 22, Issue 2, January 2018, Page: 140-147

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Facile Synthesis of Natural Anise-Based Nanoemulsions and Their Antimicrobial Activity

Polymers ◽

10.3390/polym13122009 ◽

2021 ◽

Vol 13 (12) ◽

pp. 2009

Author(s):

Ola A. Abu Ali ◽

Mehrez E. El-Naggar ◽

Mohamed S. Abdel-Aziz ◽

Dalia I. Saleh ◽

Mohamed. A. Abu-Saied ◽

...

Keyword(s):

Antimicrobial Agents ◽

Reducing Sugars ◽

Total Phenolics ◽

Antioxidant Activities ◽

Chemical Properties ◽

Geranyl Acetate ◽

Gram Negative Bacteria ◽

Facile Synthesis ◽

Gram Positive Bacteria ◽

And Chemical Properties

Anise oil was prepared in its nanoemulsion form to facilitate the penetration of microbial walls, causing microbe mortality. The penetration occurred easily owing to the reduction in its size (nm). Nanoemulsions with different concentrations of anise oil were prepared using lecithin as an emulsifying agent with the aid of an ultra-sonification process. Their morphological and chemical properties were then characterized. The promising constituents were l-Menthone (11.22%), Gurjunene (6.78%), Geranyl acetate (4.03%), Elemene (3.93%), Geranyl tiglate (3.53%), geraniol (3.48%), linalool (0.17%) as well as camphene (0.12%). Different concentrations of prepared anise oil in micro and nanoemulsions were tested as antimicrobial agents against Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli), yeast (Candida albicans) and fungi (Asperigillus niger). The findings illustrated that the anise oil-based nanoemulsion exhibited better results. Different biochemical and biological evaluations of anise oil nanoemulsions were conducted, including determining killing times, antioxidant activities (using three different methods), and total phenolics. A trial to estimate the mode of action of anise oil-based nanoemulsion as an antimicrobial agent against S. aureus and C. albicans was performed via studying the release of reducing sugars and protein and conducting scanning electron microscopy.

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ANTIBACTERIAL ACTIVITY OF BARK EXTRACTS OF FICUS GLOMERATA ROXB AGAINST SOME GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

INDIAN DRUGS ◽

10.53879/id.50.05.p0044 ◽

2013 ◽

Vol 50 (05) ◽

pp. 44-47

Author(s):

S. D. Bhinge ◽

◽

M. G Hogade ◽

A. S Savali ◽

H. R. Chitapurkar ◽

...

Keyword(s):

Antibacterial Activity ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Aqueous Extracts ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Agar Diffusion Method ◽

Ficus Glomerata ◽

And Staphylococcus Aureus

Petroleum ether, ethanol and aqueous extracts of the bark of Ficus glomerata (Urticaceae) were screened for their antibacterial activity using agar diffusion method. They were tested against six bacteria; three Gram-positive bacteria (Bacillus subtilis, Bacillus aureus and Staphylococcus aureus) and three Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa). The susceptibility of the microorganisms to the extracts of these plants was compared with each other and with selected antibiotics.

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In vitro Antimicrobial Activity of Plants used in Cambodian Traditional Medicine

The American Journal of Chinese Medicine ◽

10.1142/s0192415x07005338 ◽

2007 ◽

Vol 35 (05) ◽

pp. 867-873 ◽

Cited By ~ 9

Author(s):

Aun Chea ◽

Marie-Caroline Jonville ◽

Sok-Siya Bun ◽

Michèle Laget ◽

Riad Elias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Traditional Medicine ◽

Bactericidal Effect ◽

Gram Positive Bacteria ◽

Antibacterial And Antifungal Activities ◽

Methanolic Extracts ◽

Disk Diffusion Assay ◽

Antibacterial And Antifungal ◽

Diffusion Assay

The purpose of the present study was to screen 27 plant species used in the traditional medicine of Cambodia for in vitro antibacterial and antifungal activities. Thirty-three methanolic extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Mycobacterium smegmatis and Candida albicans. Screened by disk diffusion assay, the extracts showed antimicrobial activity especially on Gram-positive bacteria. None of the crude methanolic extracts showed activity against P. aeruginosa. Twenty-five selected extracts were evaluated using a micro-dilution test. Harrisonia perforata (roots) and Hymenodictyon excelsum (bark) exhibited a bactericidal effect against S. aureus at a concentration of 500 μg/ml. Azadirachta indica (bark), Harrisonia perforata (roots and stem) and Shorea obtusa (roots) exhibited a bactericidal effect against M. smegmatis at 250 μg/ml.

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GC-MS Analysis of Antibacterial Phytochemicals from Cassia sophera Linn

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2020.13.5.10 ◽

2020 ◽

Vol 13 (5) ◽

pp. 5131-5137

Author(s):

Dr. Shyamal K. Jash ◽

Dilip Gorai ◽

Lalan Chandra Mandal ◽

Sekhar Pal

Keyword(s):

Chemical Constituents ◽

Research Work ◽

Enterobacter Aerogenes ◽

Gas Chromatography Mass Spectrometry ◽

Gram Negative Bacteria ◽

Research Groups ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Ms Analysis ◽

The World

Cassia sophera Linn (Leguminosae) plants are widely distributed worldwide, and find immense applications in traditional systems of medicine in many countries. The plant has been partly explored by various research groups in the world. In our present study, two oils (OL-1 & OL-2) from aerial parts and roots of Cassia sophera have been evaluated by GC/MS using Perkin-Elmer Gas Chromatography–Mass Spectrometry and comparison of spectral data with the existing in National Institute of Standards and Technology (NIST) library. GC/MS analysis of the two oils revealed the presence of a number of chemical constituents. Again, the isolated oils exhibited considerable antibacterial activity against two Gram positive bacteria viz. Bacillus megaterium & Staphylococcus aureus and two Gram negative bacteria viz. Escherichia coli & Enterobacter aerogenes. Therefore, the present study will definitely inspire the researchers engaged in this direction to undertake further research work on this plant for searching new lead molecules

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Antimicrobial activity of ethanolic and aqueous extracts of medicinally used tree ferns Alsophila cuspidata and Cyathea microdonta

Acta Botanica Malacitana ◽

10.24310/abm.v42i1.2885 ◽

2018 ◽

Vol 42 (1) ◽

pp. 119-123

Author(s):

Charlie Longtine ◽

Adrian Tejedor

Keyword(s):

Antimicrobial Activity ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Aqueous Extracts ◽

Antimicrobial Compounds ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Neotropical Tree ◽

Tree Ferns

Medicinal plants are important components of traditional medicine and sources of novel antimicrobial compounds against drug resistance pathogen strains. This study tested the antimicrobial activity of ethanolic and aqueous extracts of medicinally used Neotropical tree ferns Cyathea microdonta and Alsophila cuspidata against gram positive bacteria and gram negative bacteria using the Kirby-Bauer disc diffusion method. Highest antimicrobial activity was observed in ethanolic extracts, and extracts were more active against gram positive bacteria than gram negative bacteria. No difference was observed in antimicrobial activity between species or between extracts made from the leaves, stipe, or caudex. These results suggest that the traditional use of tree fern mucilage as a topical agent for cuts and leishmaniasis lesions may reduce risk of secondary infection with skin bacteria such as Staphylococcus aureus, and that Neotropical Cyatheaceae are a potential source of novel antimicrobial compounds.

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THE EFFECT OF TERMITES EXTRACT ON INHIBITION OF GROWTH OF SOME PATHOGENIC BACTERIA AND SYNTHESIS OF BIOFILM

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51ispecial.895 ◽

2020 ◽

Vol 51 (Special) ◽

Author(s):

Saleh & et al.

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Gram Negative Bacteria ◽

Gram Positive Bacteria ◽

Inhibition Of Growth ◽

Biofilm Production ◽

Antibacterial Material ◽

Diffusion Assay

This study was aimed to extract the effective material from the dry nests of termites and detect its antibacterial activity against some pathogenic bacterial isolates and inhibit synthesis of its biofilm. Termites dry nests were collected and the effective material was extracted then the antibacterial activity was detected using the disc diffusion assay. Results were showed that the extract have antibacterial material from the Termites dry nests, this extract showed antibacterial activity against Gram positive bacteria (Staphylococcus aureus) at (21.5mm) and Gram negative bacteria ( Enterobacter sp. and Pseudomonas aeruginosa) at (26 mm and 20 mm) respectively by inhibiting their growth, as well as its effect on biofilm production of pathogenic bacteria. Staphylococcus aureus, Enterobacter sp. and Pseudomonas aeruginosa revealed a significant decrease (P<0.01) in biofilm synthesis as the concentration of the extract increased.

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Antimicrobial and antioxidant activity of the extracts from Origanum vulgare L. growing wild in Bosnia and Herzegovina

Genetics & Applications ◽

10.31383/ga.vol2iss2pp62-66 ◽

2018 ◽

Vol 2 (2) ◽

pp. 62

Author(s):

Renata Bešta-Gajević ◽

Erna Karalija ◽

Anesa Jerković-Mujkić ◽

Dženana Karadža ◽

Lejla Smajlović-Skenderagić ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Antioxidant Activities ◽

Gram Negative Bacteria ◽

Aqueous Extracts ◽

Origanum Vulgare ◽

Gram Negative

Antifungal, antimicrobial, insecticidal and antioxidant activities of Origanum vulgare L. provide the basis for suggesting that oregano plant extracts may be useful for prevention and treatment of many infection. The main goal of this study was to determine antimicrobial and antioxidant activities of methanolic and aqueous extracts from the leaves and flowers of Origanum vulgare. Antimicrobial testing of plant extracts was done using well diffusion method. Activity of extracts were tested against Gram positive bacteria: Staphylococcus aureus ATCC 25923, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212 and five Gram-negative bacteria: Salmonella abony ATCC 6017, Salmonella enterica serovar Enteritidis ATCC 31194, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 25922, extended-spectrum β-lactamase (ESBL) producing Escherichia coli ATCC 35218 and fungi Candida albicans ATCC 1023. Antibiotics ampicillin, streptomycin and antimycotic nystatin were used as positive controle. The antioxidant activity was determined by using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The highest values for inhibition zone for methanolic and aqueous extracts were recorded for Gram positive Bacillus subtilis, Staphylococcus aureus and MRSA. Methanolic extracts exhibited antibacterial activity against tested Gram negative bacteria in variable degree while the growth of these bacteria was not inhibited by aqueous extracts. Tested fungi Candida albicans was not susceptible to investigated oregano extracts. All the extracts showed moderate to potent antioxidant activity, among which the methanolic flower extract demonstrated the strongest antioxidant activity with the IC50 value of 0.205 mg/mL. Therefore it can be concluded that flower and leaf oregano extracts have great antibacterial and antioxidant potential.

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