scholarly journals Transcriptome analysis revealed the regulation of gibberellin and the establishment of photosynthetic system promote rapid seed germination and early growth of seedling in pearl millet

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Bingchao Wu ◽  
Min Sun ◽  
Huan Zhang ◽  
Dan Yang ◽  
Chuang Lin ◽  
...  
Keyword(s):  
Seed Germination ◽  
Pearl Millet ◽  
Seedling Growth ◽  
Molecular Mechanisms ◽  
Germination Rate ◽  
Expression Patterns ◽  
Early Growth ◽  
Growth Stages ◽  
Millet Seed ◽  
Key Genes

Abstract Background Seed germination is the most important stage for the formation of a new plant. This process starts when the dry seed begins to absorb water and ends when the radicle protrudes. The germination rate of seed from different species varies. The rapid germination of seed from species that grow on marginal land allows seedlings to compete with surrounding species, which is also the guarantee of normal plant development and high yield. Pearl millet is an important cereal crop that is used worldwide, and it can also be used to extract bioethanol. Previous germination experiments have shown that pearl millet has a fast seed germination rate, but the molecular mechanisms behind pearl millet are unclear. Therefore, this study explored the expression patterns of genes involved in pearl millet growth from the germination of dry seed to the early growth stages. Results Through the germination test and the measurement of the seedling radicle length, we found that pearl millet seed germinated after 24 h of swelling of the dry seed. Using transcriptome sequencing, we characterized the gene expression patterns of dry seed, water imbibed seed, germ and radicle, and found more differentially expressed genes (DEGs) in radicle than germ. Further analysis showed that different genome clusters function specifically at different tissues and time periods. Weighted gene co-expression network analysis (WGCNA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that many genes that positively regulate plant growth and development are highly enriched and expressed, especially the gibberellin signaling pathway, which can promote seed germination. We speculated that the activation of these key genes promotes the germination of pearl millet seed and the growth of seedlings. To verify this, we measured the content of gibberellin and found that the gibberellin content after seed imbibition rose sharply and remained at a high level. Conclusions In this study, we identified the key genes that participated in the regulation of seed germination and seedling growth. The activation of key genes in these pathways may contribute to the rapid germination and growth of seed and seedlings in pearl millet. These results provided new insight into accelerating the germination rate and seedling growth of species with slow germination.

scholarly journals Transcriptome Analysis Revealed the Regulation of Gibberellin and the Establishment of Photosynthetic System Promote Rapid Seed Germination and Early Growth of Seedling in Pearl Millet

2020 ◽  
Author(s):  
Bingchao Wu ◽  
Min Sun ◽  
Huan Zhang ◽  
Dan Yang ◽  
Chuang Lin ◽  
...  
Keyword(s):  
Seed Germination ◽  
Pearl Millet ◽  
Seedling Growth ◽  
Germination Rate ◽  
Expression Patterns ◽  
Early Growth ◽  
High Yield ◽  
Maintenance Cost ◽  
Key Genes ◽  
Pearl Millet Seeds

Abstract Background：Seed germination is the most important stage for the formation of a new plant. This process starts when the dry seeds begin to absorb water and ends when the radicles sticks out. The germination rate of different plant seeds varies differently. Most energy plants that usually grow on marginal land, the rapid germination of seeds is more conducive to its superiority in competition with surrounding plants, which is also the guarantee of normal plant development and high yield. Pearl millet is an important cereal crop that shares widespread applications in the world. It has the advantages of fast growth, high yield, and low maintenance cost. It can also be used to extract bioethanol to solve the increasingly prominent energy problems. Previous germination experiments are the evidence of very fast seed germination rate of pearl millet, but the molecular mechanisms behind it is still unclear.Results: Through the germination test and the measurement of the germs and radicles length of the seedlings, we found that pearl millet seeds germinated very quickly after 24 hours of swelling of the dry seeds. By using transcriptome sequencing technology, we characterized the gene expression patterns of dry seeds, water imbibed seeds, germs and radicles of seedlings, and found the more DEGs in radicles than germs. Further analysis showed that different genome clusters function specifically at different tissues and time periods. WGCNA and KEGG enrichment analysis showed that that many genes that positively regulate plant growth and development are highly enriched and expressed, especially the gibberellin signaling pathway that can promote seed germination. We speculated that the activation of these key genes promotes the germination of pearl millet seeds and the growth of seedlings. To verify this inference, we measured the content of the main effect hormone gibberellin and found that the gibberellin content after seed imbibition rose sharply and remained at a high level.Conclusions：This study explored the expression patterns of genes involved in pearl millet growth from the germination of dry seeds to the early growth stages. Also identifies the key genes involved in the regulation of seed germination and seedling growth. The activation of key genes in these pathways may contribute to the rapid germination and growth of seeds and seedlings in pearl millet. These results provide new insights to solve the problem for the plants with slow seed germination and seedling growth.

scholarly journals Impacts of different salt source and concentrations on germination and seedling growth of many pumpkin seeds used as rootstoch in Iran

Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 235-250 ◽  
Author(s):  
Ahmad Dadashpour
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Seedling Growth ◽  
Root Length ◽  
Germination Rate ◽  
Shoot Length ◽  
Root Weight ◽  
Pumpkin Seeds ◽  
High Concentrations ◽  
Shoot Weight

The effects of different salt sources (C Cl2, NaCl, and KCl) and concentrations, as measured by electrical conductivity, (0, "control", 1, 3, 5, 7 and 9 dS m-1) on seed germination and seedling growth of ?Ferro?, ?Obez?, ?RS 841? and ?Strong Tosa F1? pumpkin varieties used as rootstock were investigated in this study. The results showed that germination rate, root length, shoot length, fresh root weight, dry root weight, fresh shoot weight and dry shoot weights tend to decrease when the electrical conductivity of the solution is higher than 5 dS m-1, independent of salt sources and in all of the varieties. Three days after seeding, a germination ratio of 5 % was obtained from RS 841 variety in all salt source and concentrations, while a germination ratio over 50 % was obtained in ?Strong Tosa? variety for the same conditions except CaCl2 salt source. Nevertheless, seeds germinated in medium having high concentrations of CaCl2 had lower germination rate and poor seedling growth, compared to media having the same concentrations of NaCl and KCl. It was concluded that all of the varieties studied were more sensitive to the concentrations prepared using CaCl2 than that of the KCl, and NaCl.

scholarly journals Consequences of Seed Priming with Salicylic Acid and Hydro Priming on Smooth Vetch Seedling Growth under Water Deficiency

2017 ◽  
Vol 9 (12) ◽  
pp. 259
Author(s):  
Amin Namdari ◽  
Abolfazl Baghbani
Keyword(s):  
Salicylic Acid ◽  
Water Deficit ◽  
Seedling Growth ◽  
Glycine Betaine ◽  
Soluble Sugars ◽  
Early Growth ◽  
Water Deficit Stress ◽  
Growth Stages ◽  
Water Deficiency ◽  
Available Water

Due to low rainfall at early autumn, smooth vetch seedling growth in rain-fed lands often is limited by water deficit stress yet the data regarding the reactions of smooth vetch to water deficit at early growth stages are pretty rare. The objective of current study was to examine possibility of using priming treatments (hydro priming and priming salicylic acid) to alleviate the inhibitory effect of water deficiency during early growth of Smooth Vetch. In this respect, seeds were soaked in distilled water (hydro priming) or 0.5 mM solution of SA for 36 h at 10 °C then dried back to original moisture content. Pots were irrigated for 25 days at four levels of available water containing field capacity (FC), 75% FC, 50% FC and 25% FC. In general, seedling emergence and early growth were markedly limited by increasing water deficiency. However, priming treatments particularly with SA caused considerable improvement in either emergence or growth of seedlings (dry weight, length). The obtained results showed that primed samples exhibited higher accumulation of proline, glycine betaine (GB) under all levels of available water except 100% FC and also higher total soluble sugars (TSS) and trehalose under severe water deficit (25% FC). SA primed samples had higher relative water content especially under higher levels of water deficiency. The more balanced water status within SA primed samples also was accompanied with higher accumulation of proline and glycine betaine. There were significant differences between two priming treatments in terms of proline and GB content within seedlings and SA priming considerably increased proline and GB accumulation. In contrast to proline and GB, TSS and trehalose content wasn’t influenced by SA treatment and both hydro and SA primed samples showed statistically similar quantities.

scholarly journals Unique expression patterns of multiple key genes associated with the evolution of mammalian flight

2014 ◽  
Vol 281 (1783) ◽  
pp. 20133133 ◽  
Author(s):  
Zhe Wang ◽  
Mengyao Dai ◽  
Yao Wang ◽  
Kimberly L. Cooper ◽  
Tengteng Zhu ◽  
...  
Keyword(s):  
Regulatory Networks ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Genetic Changes ◽  
Multiple Gene ◽  
Membrane Growth ◽  
Complex Integration ◽  
Key Genes ◽  
Bat Wing

Bats are the only mammals capable of true flight. Critical adaptations for flight include a pair of dramatically elongated hands with broad wing membranes. To study the molecular mechanisms of bat wing evolution, we perform genomewide mRNA sequencing and in situ hybridization for embryonic bat limbs. We identify seven key genes that display unique expression patterns in embryonic bat wings and feet, compared with mouse fore- and hindlimbs. The expression of all 5′HoxD genes ( Hoxd9–13 ) and Tbx3 , six known crucial transcription factors for limb and digit development, is extremely high and prolonged in the elongating wing area. The expression of Fam5c , a tumour suppressor, in bat limbs is bat-specific and significantly high in all short digit regions (the thumb and foot digits). These results suggest multiple genetic changes occurred independently during the evolution of bat wings to elongate the hand digits, promote membrane growth and keep other digits short. Our findings also indicate that the evolution of limb morphology depends on the complex integration of multiple gene regulatory networks and biological processes that control digit formation and identity, chondrogenesis, and interdigital regression or retention.

Japanese Foxtail (Alopecurus japonicus) Management in Wheat in China: Seed Germination, Seedling Emergence, and Response to Herbicide Treatments

2017 ◽  
Vol 32 (2) ◽  
pp. 211-220 ◽  
Author(s):  
Hengzhi Wang ◽  
Yizhao Huang ◽  
Lele Zhang ◽  
Weitang Liu ◽  
Jinxin Wang
Keyword(s):  
Seed Germination ◽  
Integrated Management ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Integrated Approach ◽  
Growth Stages ◽  
Ph Values ◽  
Grass Weed ◽  
Different Growth Stages

AbstractLittle information is published related to seed germination and seedling emergence of Japanese foxtail, a troublesome annual grass weed widely distributed in winter wheat fields in China. Three Japanese foxtail populations were studied under laboratory and greenhouse conditions, to determine the effects of different environmental factors on seed germination or seedling emergence. Chemical control is absolutely necessary in integrated management, and efficacy of POST herbicides against different growth stages of Japanese foxtail was evaluated. Germination rate was 90% or more when temperature ranged from 5 to 25 C, with germination onset shortened as temperature increased. Light was not required for germination to occur. For pH values ranging from 5 to10 there was no effect on seed germination. Japanese foxtail seed germination was sensitive to osmotic stress and completely inhibited at an osmotic potential of -1.1 MPa. The ‘1513’ population of Japanese foxtail demonstrated tolerance to soil salinity, with 98% germination at 80 mM NaCl compared with 25 and 40% germination for populations ‘1532’ and ‘1544’, respectively. High amounts of crop residue (10 t ha−1) suppressed Japanese foxtail emergence 38 to 55%. Germination of seeds placed at 160 C for 5 min was completely inhibited for dry seeds, with a similar effect at 130 C for pre-soaked seeds. Seed burial in the soil from 0 to 4 cm had no effect on seedling emergence, but burial at 7 cm completely inhibited seedling emergence. POST herbicides mesosulfuron-methyl (13.5 g ai ha−1), clodinafop-propargyl (67.5 g ai ha−1), pyroxsulam (13.5 g ai ha−1), pinoxaden (67.5 g ai ha−1) and isoproturon (1125 g ai ha−1) reduced plant dry weight 80% or more when applied at three- to seven-leaf stage, but control declined with application at later growth stages. The information from this study helps to develop an integrated approach to Japanese foxtail management.

scholarly journals Elucidation of seed dormancy and phytohormones by germination after exogenous foliage whey application

1970 ◽  
pp. 08-14
Author(s):  
Jadhav Rajesh K, Pranay Mulam, Khot Vidhesh
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seedling Growth ◽  
Rapid Growth ◽  
Germination Rate ◽  
Exogenous Application ◽  
Paper Towel ◽  
Leguminous Seeds ◽  
Growth Of Seedlings ◽  
Leaf Juice

A study was conducted with an aim of achieving 100% seed germination and to evaluate the rate of dormancy upon exogenous application of the deproteinised leaf juice (DPJ) in various leguminous and non leguminous seeds. DPJ if inducing retardation of dormancy, the activity of enzyme was analysed to prove the presence of phytohormones. DPJ from the three non leguminous foliages of brinjal, radddish and dasheen were prepared by green crop fractionation. The seed germination results obtained by the paper towel method and the activity of enzyme protease in DPJ of radish and Colocasia leaves separately. There was variation in germination rate by different DPJ influence. Some seeds showed fast germination while some germinated late. Few seeds were not germinated by control. In some seeds, DPJ decreased the rate of germination while on the contrary there was rapid growth of seedlings by the potentiality of DPJ. DPJ found mutagenic by its influencing inhibitory expression in seedling growth in some cases. The enzyme protease released by phytohormone gibberellin during the seed germination. Positive glyoxlic test indicated presence of auxins in whey.

scholarly journals ​Influence of Temperature and Salinity Stress on Seed Germination and Seedling Growth of Soybean (Glycine max L.)

2021 ◽  
Author(s):  
Mustafa Çirka ◽  
Ali Rahmi Kaya ◽  
Tamer Eryiğit
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Rate ◽  
Petri Dish ◽  
Negative Effects ◽  
Randomized Design ◽  
Salt Level ◽  
Glycine Max L ◽  
Early Seedling ◽  
Lower Temperature

Background: The high salt level of a germinating environment can lead to reduced, delayed, and even complete inhibition of germination and seeding growth due to osmotic action and/or ion toxicity. Based on this viewpoint, the aim of this study was to investigate germination temperature and salinity effects on germination and early seedling growth of soybean, which can be sown as first and second crops. Methods: Soybean seeds were subjected to NaCl induced saline germinating media prepared in petri dishes under two different germination temperatures (20±2 and 25±2oC). Thirty sterilized seeds per petri dish were sown in ten salt treatments (0, 100, 150, 250, 750, 1000, 2250, 5000, 7500 and 10000 ppm NaCl L-1). The study was carried out according to the completely randomized design with four replications. Result: Lower temperature promoted seed germination, while the high temperature significantly inhibited the seed germination at all NaCl doses tested. As a result, 25oC temperatures, which can only be measured at the time of the second sowing, have been found to negatively affect germination and also increase the negative effects of salt. Due to the moderate tolerance of soybeans to salt stress, the germination rate was positively affected up to 750 ppm NaCl L-1 dose and resulted in severe reductions in subsequent doses. Also, the tolerance of soybean was negatively influenced by the interaction of temperature and NaCl concentration.

scholarly journals Responses of Maize Varieties to Salt Stress in Relation to Germination and Seedling Growth

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Nuurismaan Hassan ◽  
Md. Kamrul Hasan ◽  
Md. Obaidullah Shaddam ◽  
Mohammad Sohidul Islam ◽  
Celaleddin Barutçular ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Agricultural Research ◽  
Growth Parameters ◽  
Tap Water ◽  
Germination Rate ◽  
Dry Weight ◽  
Growth Stages ◽  
Water Control ◽  
Maize Varieties

A pot experiment was carried out at the Laboratory of Department of Agronomy, Hajee Mohamad Danesh Science and Technology University (HSTU), Bangladesh during 2016 to evaluate the response of maize varieties at germination and seedling growth stages under salt stress. The seeds of the BARI (Bangladesh Agricultural Research Institute) developed four maize varietiesviz.Barnali, Khoi Vutta, Mohor and BARI Maize 5 were placed in plastic pots (each of 25 cm length and 12 cm width) on sand bed irrigated with tap water (control), 100 and 200 mM NaCl salt solutions. It was replicated in thrice with completely randomized design (CRD). Salinity stress significantly affected the germination characters and seedling growth parameters of maize varieties. The germination percentages (GP) and germination rate (GR) reduced significantly with increasing salinity, and the variety Khoi Vutta showed the highest GP and GR followed by Barnali and Mohor showed the lowest GP and GR followed by BARI maize 5. Under high salinity level, seedling growths characteristics like shoot and root lengths, fresh and dry weight of shoot and roots reduced remarkably in the variety Mohor indicating salt susceptible while the minimum reduction of the aforementioned traits was observed in the variety Khoi Vutta demonstrating high salt tolerant variety. The studied varieties can be ranked on the basis of salt tolerance as Khoi Vutta > Barnali > BARI Maize 5 > Mohor from the experiment.

scholarly journals The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity Stress

2012 ◽  
Vol 76 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Maryam Haghighi ◽  
Zahra Afifipour ◽  
Maryam Mozafarian
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Tomato Seeds ◽  
Germination Characteristics ◽  
Salinity Levels ◽  
Mean Germination Time

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.

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