Efficacy of Bravecto® Plus spot-on solution for cats (280 mg/ml fluralaner and 14 mg/ml moxidectin) for the prevention of aelurostrongylosis in experimentally infected cats

Abstract Background The feline lungworm Aelurostrongylus abstrusus affects the lower respiratory tract in cats worldwide. As infections may lead to chronic respiratory changes or even death, preventive treatment in cats with outdoor access is warranted. Methods The preventive efficacy of a spot-on solution (Bravecto® Plus spot-on solution for cats, MSD) against cat aelurostrongylosis was evaluated using three different preventive treatment regimes in a negative controlled, randomized and partially blinded laboratory efficacy study with 31 purposed-bred cats. The minimum recommended dose of 2.0 mg moxidectin + 40 mg fluralaner/kg bodyweight was applied once 12 (Group [G]1), 8 (G2) or 4 (G3) weeks before experimental infection with 300 third-stage larvae (L3) of A. abstrusus. Another group served as untreated control (G4). Individual faecal samples were analysed as of day 30 post infection (pi) to monitor larvae excretion. Necropsy was performed at days 47–50 pi. The lungs were examined macroscopically for pathological findings and (pre-)adult worms were counted to assess preventive efficacy. Results Beginning at day 32–40 pi, all cats of the control group were constantly shedding larvae of A. abstrusus, whereas only one animal of G1 excreted larvae at several consecutive days. In addition, two cats of G1 and G3 and three of G2 were positive on a single occasion. The geometric mean (GM) of the maximum number of excreted larvae was 7574.29 in the control group compared to 1.10 (G1), 1.19 (G2) and 0.53 (G3), resulting in a GM reduction of > 99.9% in all treatment groups. All lungs of the control animals showed severe or very severe alterations at necropsy, while in 94.44% of the treated cats lung pathology was rated as absent or mild. The GM number of (pre-)adult A. abstrusus retrieved from the lungs was 26.57 in the control group, 0.09 in G1 and 0.00 in G2 and G3. Thus, GM worm count reduction was 99.66% in G1 and 100% in G2 and G3. Conclusions A single application of Bravecto® Plus spot-on solution at a dose of 2.0 mg moxidectin + 40 mg fluralaner/kg bodyweight reliably prevents cat aelurostrongylosis for at least 12 weeks.

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Comparative preventive efficacy of ProHeart® 12, Heartgard® Plus and Interceptor® Plus against a macrocyclic lactone-resistant strain (JYD-34) of heartworm (Dirofilaria immitis) in dogs

Parasites & Vectors ◽

10.1186/s13071-021-04708-3 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Tom L. McTier ◽

Susan Holzmer ◽

Kristina Kryda ◽

Sean Mahabir ◽

John W. McCall ◽

...

Keyword(s):

Resistant Strain ◽

Dirofilaria Immitis ◽

Macrocyclic Lactone ◽

Geometric Mean ◽

Treated Group ◽

Control Group ◽

Placebo Control ◽

Worm Count ◽

Preventive Efficacy ◽

The Mean

Abstract Background The current studies compared ProHeart® 12, Heartgard® Plus and Interceptor® Plus for preventive efficacy against JYD-34, a macrocyclic lactone (ML)-resistant strain of Dirofilaria immitis in dogs. Methods In two studies, each using 24 adult beagles, dogs were allocated to four treatment groups (n = 6): placebo-treated control; ProHeart 12 as per label (0.5 mg/kg moxidectin); Heartgard Plus (HGP) as per label (minimum 6 µg/kg ivermectin); and Interceptor Plus (INP) as per label (minimum 0.5 mg/kg milbemycin oxime). In both studies, ProHeart 12 was administered as a single subcutaneous dose on day 0, and HGP and INP were administered orally on days 0, 30, 60, 90, 120 and 150. In Studies 1 and 2, dogs were inoculated with 50 third-stage heartworm larvae (JYD-34 strain) on days −30 and 165, respectively. In Study 2, treatment for both HGP and INP was continued on days 180, 210, 240, 270, 300 and 330. Adult heartworm recoveries were performed on day 185 in Study 1 and on day 360 in Study 2. Results In Studies 1 and 2, all placebo-treated dogs developed adult heartworm infections (geometric mean, 29.9 and 34.9 worms/dog, respectively). A single dose of ProHeart 12 was 100% effective in preventing the development of adult JYD-34 heartworms when treatment was initiated 30 days after heartworm inoculation, while six consecutive monthly doses of HGP and INP were only 10.5% and 14.6% effective, respectively. The mean worm count for the ProHeart 12-treated group was significantly lower (P < 0.0001) than that for the placebo control, HGP- and INP-treated groups. In Study 2, the dogs treated with ProHeart 12 had an efficacy of 98.3%. All dogs treated with HGP and INP for 12 consecutive months had adult heartworms with efficacies of 37.7% and 34.9%, respectively. The mean worm count for the ProHeart 12-treated dogs was significantly lower (P < 0.0001) than those for the control group, HGP- and INP-treated groups. Conclusions A single administration of ProHeart 12 was 98–100% effective in preventing the development of the ML-resistant JYD-34 heartworm strain and was significantly better than multiple consecutive monthly doses of either Heartgard Plus or Interceptor Plus in both studies. Graphic Abstract

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Lack of response to garlic fed at different dose rates for the control of Haemonchus contortus in Merino wether lambs

Animal Production Science ◽

10.1071/an09057 ◽

2009 ◽

Vol 49 (12) ◽

pp. 1093 ◽

Cited By ~ 2

Author(s):

V. J. Strickland ◽

J. S. Fisher ◽

W. T. Potts ◽

G. W. Hepworth

Keyword(s):

Feed Intake ◽

Haemonchus Contortus ◽

Body Condition Score ◽

Control Group ◽

Feed Conversion ◽

Worm Count ◽

Treatment Groups ◽

Dose Rates ◽

Voluntary Feed Intake ◽

Over Time

With the increased incidence of parasite resistance to chemical anthelmintics worldwide novel approaches to manage parasite infection, such as medicinal plants and their extracts, are being investigated by the scientific community. The current study tested the effect of three rates of garlic (0.9, 1.8 and 3.6%) in a pelleted ration on Haemonchus contortus in sheep. Thirty-nine Merino wether lambs aged 6 months were divided into five treatment groups, including three garlic dose rates and two control groups that received no garlic. All animals were infected with 4000 L3 H. contortus larvae 3 weeks after allocation to treatments. A positive control group was drenched with abamectin 28 days after infection. The synthetic drench was effective in controlling the parasites, but there was no reduction in either worm egg counts (WEC) or total worm count due to the garlic. The 3.6% garlic treatment had significantly lower (P < 0.05) liveweight, feed intake, body condition score and feed conversion ratio than any of the other treatment groups, suggesting that this level of garlic had a low level of anti-nutritional properties. There was an interaction between faecal WEC and voluntary feed intake over time, with the animals with higher voluntary feed intake having lower WEC over time.

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PSVII-13 Effects of corn supplementation on muscle microRNA profiles within horses

Journal of Animal Science ◽

10.1093/jas/skz258.627 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 310-311

Author(s):

Clarissa M Carver ◽

Tanja Hess ◽

Jason Bruemmer ◽

Stephen Coleman ◽

Gabriele Landolt

Keyword(s):

Expression Profiles ◽

Geometric Mean ◽

Gluteus Medius ◽

Basal Diet ◽

Control Group ◽

Treatment Groups ◽

Mirna Expression Profiles ◽

Grass Hay ◽

Mixed Grass ◽

Endogenous Mirnas

Abstract The objective of this study was to determine the effect of corn supplementation on muscle microRNA (miRNA) profiles. Twelve mares were blocked by weight and BCS and assigned to one of two treatments (6 hd/treatment): 1) control, (basal diet: 9 Kg/hd/d of chopped mixed grass hay and ad libitum mixed grass hay), 2) basal diet supplemented with 454 g/d steam flaked corn individually using feed bags. All mares were placed on the basal diet and in the same pen 14 days prior to the beginning of corn supplementation. Mares were weighed and BCS on d -7 and 28. Muscle biopsies of the gluteus medius were taken from all horses on d0 and d26. Muscle samples were analyzed using real time RT-qPCR for 277 endogenous miRNAs. Raw CT values were normalized with the geometric mean of three consistently appearing endogenous miRNAs. Sixteen miRNAs that consistently appeared in at least 8 horses including 3 relating to metabolic disorders appearing at d0, d26, or both were analyzed. Differential abundances of miRNAs using log transformed fold change and differences between BW and BCS of treatment groups were determined by ANOVA and LS means analysis. There was no difference between BW (P < .05) and BCS (P < .05) between the two treatment groups. Feeding corn lead to higher quantities (P < .05) of mir 133a, 1515p, 6155p, 770, and 99b within muscle compared to control group. Lower quantities of mir 382 and 433 were found within muscle of horses fed corn compared to the control group (P < .05). Based upon the results of this study corn supplementation appears to influence endogenous miRNA expression profiles within muscle of horses.

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Vaccination with an Ostertagia ostertagi Polyprotein Allergen Protects Calves against Homologous Challenge Infection

Infection and Immunity ◽

10.1128/iai.72.5.2995-3001.2004 ◽

2004 ◽

Vol 72 (5) ◽

pp. 2995-3001 ◽

Cited By ~ 33

Author(s):

Isabel Vercauteren ◽

Peter Geldhof ◽

Jozef Vercruysse ◽

Iris Peelaers ◽

Wim Van Den Broeck ◽

...

Keyword(s):

Geometric Mean ◽

Single Copy ◽

Control Group ◽

Female Adult ◽

Ostertagia Ostertagi ◽

Effector Cells ◽

Third Stage ◽

Specific Immunoglobulin ◽

Native Antigen ◽

Homologous Challenge

ABSTRACT As an alternative to antihelminthic drugs, we are exploiting vaccination to control infections with the abomasal nematode Ostertagia ostertagi in cattle. Our focus for vaccine targets is excretory-secretory (ES) products of this parasite. One of the most abundant antigens in larval and adult Ostertagia ES products is a protein homologous to nematode polyprotein allergens. We found that the Ostertagia polyprotein allergen (OPA) is encoded by a single-copy gene. OPA comprises three or more repeated units, and only the 15-kDa subunits are found in ES products. The native antigen is localized in the intestinal cells of third-stage larvae and in the hypodermis and cuticle of fourth-stage larvae and adult parasites. Vaccination of cattle with native OPA (nOPA) in combination with QuilA resulted in protection against Ostertagia challenge infections. The geometric mean cumulative fecal egg counts in the nOPA-vaccinated animals were reduced by 60% compared to the counts in the control group during the 2-month course of the experiment. Both male and female adult worms in nOPA-vaccinated animals were significantly shorter than the worms in the control animals. In the abomasal mucus of vaccinated animals the nOPA-specific immunoglobulin G1 (IgG1) and IgG2 levels were significantly elevated compared to the levels in the control animals. Reductions in the Ostertagia egg output and the length of the adult parasites were significantly correlated with IgG1 levels. IgG2 titers were only negatively associated with adult worm length. Protected animals showed no accumulation of effector cells (mast cells, globular leukocytes, and eosinophils) in the mucosa. In contrast to the native antigen, recombinant OPA expressed in Escherichia coli did not stimulate any protection.

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A Controlled Study of the Effect of Therapies Aimed at Adolescent and Family Psychopathology in Anorexia Nervosa

The British Journal of Psychiatry ◽

10.1192/bjp.159.3.325 ◽

1991 ◽

Vol 159 (3) ◽

pp. 325-333 ◽

Cited By ~ 186

Author(s):

A. H. Crisp ◽

Kingsley Norton ◽

Simon Gowers ◽

Christine Halek ◽

Carol Bowyer ◽

...

Keyword(s):

Anorexia Nervosa ◽

Weight Gain ◽

Developmental Psychopathology ◽

Controlled Study ◽

Control Group ◽

Treatment Groups ◽

Treatment Regimes ◽

Body Weights ◽

One Year ◽

Family Psychopathology

Ninety patients with severe anorexia nervosa fulfilling DSM–III–R criteria were assessed in depth in terms of their family developmental psychopathology and then randomly allocated to either one of three treatment groups or to no treatment. In three treatment regimes, a behavioural approach to diet and weight gain was coupled with individual and family psychotherapy directed at the adolescent maturational problems. All three treatment regimes were highly significantly effective at one year in terms of weight gain, return of menstruation, and aspects of social and sexual adjustment. Body weights above those at pubertal onset were achieved for the group mean maximum and one-year follow-up weights for all three treatment groups but not the control group.

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Synbiotic administration of canine-derived strain Lactobacillus fermentum CCM 7421 and inulin to healthy dogs

Canadian Journal of Microbiology ◽

10.1139/cjm-2012-0472 ◽

2013 ◽

Vol 59 (5) ◽

pp. 347-352 ◽

Cited By ~ 4

Author(s):

Viola Strompfová ◽

Andrea Lauková ◽

Dušan Cilik

Keyword(s):

Healthy Adult ◽

Ph Value ◽

Ammonia Concentration ◽

Lactobacillus Fermentum ◽

Microbial Populations ◽

Control Group ◽

Treatment Groups ◽

Laxative Effect ◽

Faecal Samples ◽

Healthy Dogs

The canine-derived strain Lactobacillus fermentum CCM 7421 has been demonstrated to exert certain health benefits as a probiotic in dogs. Synbiotic combinations are widely used but are rarely studied in dogs. In this study the prebiotic inulin in combination with L. fermentum CCM 7421 was tested for its effects on faecal microbial populations, faecal characteristics, and blood biochemistry in canine experiments. Healthy adult dogs (n = 36) were randomly assigned to 1 of 3 treatment groups (12 dogs/treatment): (i) the control group (C), (ii) the probiotic group (LF group: L. fermentum CCM 7421, 108 CFU/mL of Ringer buffer, 0.1 mL/kg of body mass), and (iii) the synbiotic group (LF+I group: L. fermentum CCM 7421 + inulin (I; Raftifeed IPS) added as 1% of diet). The experiment lasted for 7 weeks with a 2-week treatment period. We detected a significant increase of lactic acid bacteria (LF versus C, day 7; LF versus C and LF versus LF+I, days 28 and 49), a decrease of clostridia (LF versus C, day 14), a lower pH value (LF versus LF+I, day 28), and a higher ammonia concentration (LF versus LF+I, days 14 and 49) in faecal samples. The synbiotic LF+I combination did not intensify the probiotic L. fermentum CCM 7421 efficacy, but its slight laxative effect can be useful to prevent constipation, e.g., in senior dogs.

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Pengaruh Konsumsi Sari Buah Jambu Merah dan Madu terhadap Kenaikan Nilai HB pada Ibu Hamil di Tempat Praktek Mandiri Bidan Muarofah Surabaya

WIRARAJA MEDIKA ◽

10.24929/fik.v10i1.938 ◽

2020 ◽

Vol 10 (1) ◽

pp. 36-40

Author(s):

Ratna Indriyani ◽

Aulia Aulia ◽

Maulana Widi Andrian ◽

Emdat Suprayitno

Keyword(s):

Pregnant Women ◽

Preventive Treatment ◽

Wilcoxon Test ◽

Average Level ◽

Control Group ◽

Treatment Groups ◽

Before And After ◽

Post Test ◽

Equivalent Control ◽

Pregnant Mothers

ABSTRAK Pregnancy is one of the Physiology things but, in the process of pregnancy not a little pregnant mothers that experienced the problem like Anemia disease. The Aim of the research is To Known the effect in giving the red Guava juice and honey into the e knhancement HB level for the Pregnant Women in semester two and semester Three by the Light anemia. The method employed in this research was Experimental Quasy by Up Design Non equivalent Control Group pre test and post test design . the instrument or the sample of this research is the pregnant mothers in semester two and semester three consists of 32 as informants and they are will divide into the two groups as follows: 16 Respondent in treatment groups and 16 respondent in control groups. The technique that used in this research was accidental sampling. The data was collected by using observation task. The data will analyse by using Wilcoxon test. The result of the research that the level of Pregnant mother’s HB before Giving the Red Guava Juice and honey is in the average level of 9,81. The HB level of pregnant mother’s after Giving the Red Guava Juice and honey most of the levels of Pregnant Mother’s HB was increased in the treatment group in the average level of 10,25 gr %, while in the control group have no found the differentiated of the HB level before and after the treatment in average level of 9,81 gr % by the result of the Wilcoxon test analyse p=0,000 (p<0,05). Based on the previous analysis above writer would like to draw some conclusion as follows: the sicnificant influence in giving the Red Guava Juice and honey to increase the HB levels of pregnan mother’s. its expected all the pregnant women to Check up routine about the HB level to knowing early the Anemia’s effect in Pregnancy so that it cawill be taken by the Preventive treatment Keyword: Anemia, Hb, pregnant mother

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RELATION OF THE MARKERS OF MYOCARDIAL DYSFUNCTION AND SYSTEMIC INFLAMMATION WITH STRUCTURAL AND FUNCTIONAL PARAMETERS OF THE LUNGS IN MYOCARDIAL INFARCTION PATIENTS

CARDIOVASCULAR THERAPY AND PREVENTION ◽

10.15829/1728-8800-2018-2-24-28 ◽

2018 ◽

Vol 17 (2) ◽

pp. 24-28

Author(s):

O. M. Polikutina ◽

Y. S. Slepynina ◽

E. D. Bazdyrev ◽

V. N. Karetnikova ◽

O. L. Barbarach

Keyword(s):

Myocardial Infarction ◽

Systemic Inflammation ◽

Myocardial Dysfunction ◽

Control Group ◽

Chronic Obstructive ◽

Lung Pathology ◽

Functional Changes ◽

Functional Parameters ◽

Lung Capacity ◽

Copd Patients

Aim. To evaluate the structural and functional changes in the lungs of ST elevation myocardial infarction (STEMI) patients with absence or presence of chronic obstructive lung disease (COPD), and the relation with myocardial dysfunction and systemic inflammation.Material and methods. Totally, 189 STEMI patients included: group 1 — STEMI with COPD of moderate and mild grade, 2 — STEMI with no lung pathology. Groups were comparable by clinical and anamnestic parameters. Assessment of lung function and blood collection were done at 1012 day of STEMI. For comparison of the parameters representing structural and functional changes in the lungs and comparison of Creactive protein (CRP), Nterminal probrain natriuretic peptide (NTproBNP) concentration, a control group was formed with no pulmonary pathology, comparable by age and sex with the STEMI patients.Results. In COPD patients, higher values revealed of the parameters representing the part of residual volumes in pulmonary structure. Higher residual volume (RV) was found also in STEMI and no COPD comparing to controls, however the relation RV/TLC (total lung capacity) was not higher than normal range. In both groups there were lower values of diffusion lung capacity (DLCO) comparing to controls. The lowest DLCO found in COPD patients. Concentration of NTproBNP (H=41,6; p<0,001) and CRP (H=38,6; p<0,001) in COPD was significantly higher in STEMI with no COPD patients than in controls. The negative correlations found for NTproBNP and CRP with forced expiratory volume 1 sec, FEV/FVC1, DLCO, and positive — with the values of thoracic volume, RV/TLC.Conclusion. In STEMI patients the increase revealed of residual lung volumes. Mostly the level of residual volumes is high in STEMI and COPD patients. There are associations of NTproBNP and CRP with structural and functional parameters of the lungs regardless of COPD.

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Changes of IL-4 and IFN-g expression in monocytes and T-helper lymphocytes while modeling of systemic juvenile idiopathic arthritis in Wistar rats

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2018.02.61-69 ◽

2018 ◽

pp. 61-69

Author(s):

В.Н. Сахаров ◽

П.Ф. Литвицкий ◽

Е.И. Алексеева ◽

Н.А. Маянский ◽

Р.Ш. Закиров

Keyword(s):

Juvenile Idiopathic Arthritis ◽

Peripheral Blood Mononuclear Cells ◽

Wistar Rats ◽

Mononuclear Cells ◽

Systemic Juvenile Idiopathic Arthritis ◽

Control Group ◽

Peripheral Blood Mononuclear ◽

Treatment Groups ◽

Blood Mononuclear Cells ◽

T Helpers

Цель исследования - изучение перепрограммирования мононуклеарных лейкоцитов на модели системного ювенильного идиопатического артрита (сЮИА), воспроизводимой у крыс Wistar с использованием полного адъюванта Фрейнда и липополисахарида. Методика. сЮИА воспроизведен у 6-месячных крыс-самцов Wistar. На 40-е сут. эксперимента животные были разделены на 3 группы: 1-я группа - контроль; 2-я - группа доксициклина; 3-я - группа дексаметазона. Взятие проб крови у животных проводили на нулевые, 41-е и 55-е сут. Мононуклеарные клетки периферической крови выделяли гравиметрически, после чего окрашивали их на маркеры и внутриклеточные цитокины. Дифференцировали моноциты (CD3-CD4+) и Т-хелперы (CD3+CD4+). Анализировали динамику внутриклеточной экспрессии интерлейкина IL-4 (рассматривали как маркер про-М2 фенотипа, так как в случае выделения из клетки ИЛ-4 служит стимулятором М2 поляризации макрофагов) и IFN-g (как маркер про-М1 фенотипа) по данным проточной цитофлуориметрии. Применяли непараметрический статистический тест Mann-Whitney-Wilcoxon в программе R для статистической обработки данных. Результаты и заключение. При моделировании сЮИА выявлено закономерное изменение фенотипа моноцитов. Применение же доксициклина и дексаметазона приводило к более ранней поляризации их по про-М2-пути в отношении моноцитов (на 41-е сут.) в сравнении с контролем. Про-М1 эффект (на 55-е сут., в сравнении с контролем) выявлен также в группах доксициклина и дексаметазона. У животных разных групп обнаружены характерные динамические изменения внутриклеточной экспрессии цитокинов. Важно, что различная направленность поляризации фенотипа при сЮИА и применении препаратов наблюдается не только у моноцитов, но и у Т-хелперов. The study objective was to evaluate targeted reprogramming of peripheral blood mononuclear cells in systemic juvenile idiopathic arthritis (sJIA) modeled in 6-month-old male Wistar rats by co-administration of complete Freund’s adjuvant and lipopolysaccharide. Methods. On day 40 of the experiment, rats were divided into three groups: control, doxycycline, and dexamethasone groups. Blood samples were collected on days 0, 41, and 55. Peripheral blood mononuclear cells were isolated gravimetrically and stained for markers and cytokines. Monocytes (CD3-CD4+) and T-helpers (CD3+CD4+) were differentiated as target cells. IL-4 was considered a marker for the pro-M2 phenotype since IL-4 can activate M2 macrophage polarization; IFN-g was considered a marker for the pro-M1 phenotype. Time-related changes in the intracellular expression of IL-4 and IFN-g were studied using flow cytometry. Data were analyzed using nonparametric statistical tests (Mann-Whitney-Wilcoxon) in the R environment for statistical computing. Results and conclusions. Monocytes (like macrophages) underwent reprogramming during the development of modeled sJIA disease. In monocytes of doxycycline and dexamethasone treatment groups, pro-M2 effects were observed earlier (day 41) than in the control group. Pro-M1 effects were observed in monocytes of doxycycline and dexamethasone groups on day 55, as compared with the control group. Characteristic time-related changes of intracellular cytokine expression were described for different groups. Importantly, the differently directed phenotype polarization was observed in sJIA and treatment groups for both monocytes and T-helpers.

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Mechanism Involved in Fortification by Berberine in CDDP-Induced Nephrotoxicity

Current Molecular Pharmacology ◽

10.2174/1874467213666200220142202 ◽

2020 ◽

Vol 13 (4) ◽

pp. 342-352 ◽

Cited By ~ 1

Author(s):

Vipin K. Verma ◽

Salma Malik ◽

Ekta Mutneja ◽

Anil K. Sahu ◽

Kumari Rupashi ◽

...

Keyword(s):

Oxidative Stress ◽

Renal Tissue ◽

Isoquinoline Alkaloid ◽

Experimental Models ◽

Beclin 1 ◽

Control Treatment ◽

Control Group ◽

Treatment Groups ◽

Single Intraperitoneal Injection ◽

Male Wistar Rats

Background: The activation of Nrf2/HO-1 pathway has been shown to protect against cisplatin- induced nephrotoxicity by reducing oxidative stress. Berberine (Ber), an isoquinoline alkaloid, has demonstrated antioxidant, anti-inflammatory and anti-apoptotic activities in various experimental models. Aim: To check the effect of Ber on cisplatin-induced nephrotoxicity and to explore the involved mechanism. Methods: Adult male Wistar rats were divided into 6 groups: Normal, cisplatin-control, treatment groups and per se group. Normal saline and Ber (20, 40 and 80 mg/kg; p.o.) was administered to rats for 10 days. A single intraperitoneal injection of cisplatin (8 mg/kg) was injected on 7th day to induced nephrotoxicity. On 10th day, rats were sacrificed, the kidney was removed and stored for the estimation of various parameters. Results: As compared to cisplatin-control group, Ber pretreatment improved renal function system and preserved renal architecture. It also diminished oxidative stress by upregulating the expression of Nrf2/HO-1 proteins. In addition, Ber attenuated the cisplatin mediated inflammation and apoptosis. Furthermore, it also reduced the phosphorylation of p38/JNK and PARP/Beclin-1 expression in the kidney. Conclusion: Ber attenuated renal injury by activating Nrf2/HO-1 and inhibiting JNK/p38MAPKs/ PARP/Beclin-1 expression which prevented oxidative stress, inflammation, apoptosis and autophagy in renal tissue.

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