The lipid metabolome of clear cell renal cell carcinoma (CCRCC).

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 10609-10609
Author(s):  
Saby George ◽  
Kyoung-Soo Choi ◽  
Roberto Pili ◽  
Abdul Latif Kazim
Keyword(s):  
Kidney Cancer ◽  
Structural Information ◽  
Charge Ratio ◽  
Control Group ◽  
P Value ◽  
Detailed Knowledge ◽  
Butyl Ether ◽  
Cell Renal Cell Carcinoma ◽  
Lipid Species ◽  
And Control

10609 Background: The commonest type of kidney cancer is CCRCC. Treatment approaches mostly target aberrant vasculature. However, kidney cancer is also known to accumulate lipids and a detailed knowledge of the lipid species present in these tumors could lead to a better understanding of the underlying aberrant metabolic pathways and suggest possible treatment strategies. Lipidomics is an emerging field driven by rapid advances in mass spectrometry (MS), and is widely used to discover biomarkers. We attempt to identify the lipidomic profile of CCRCC using a liquid chromatography MS-based approach (LC-MS). Methods: We utilized 6 fresh frozen representative samples of CCRCC and matching non-tumor areas of kidney from nephrectomy samples. Lipids and other non-polar cellular constituents were extracted from both CCRCC and control tissues by methyl-t-butyl ether /methanol. LC-MS based lipid profiling was performed on a Waters Q-ToF Premier MS coupled with Ultra Performance LC. The peak detection and alignment across all chromatograms were performed using the XCMS software (v 1.14.1, Scripps Center for Metabolomics). Statistical comparisons of the intensities of aligned peaks were performed using the XCMS-built-in Welch's t-test. Results: The outcome of XCMS was converted to a table that contains fold change, p-value and mass to charge ratio (m/z) for each peak, its corresponding retention time, and the integrated peak intensities from all samples. 224 peaks out of 1419 differed between CCRCC and the control group, with p <0.05, calculated by XCMS. About an equal number of analytes increased or decreased in CCRCC compared with control samples. Preliminary attempts to identify the analytes included use of METLIN (Scripps Center for Metabolomics) and HMDB (Human metabolome database, Genome Alberta & Genome Canada) databases. Many of the hits identified phosphatidylcholines, phosphatidylethanolamines, triacylglycerols and diacylglycerols, as well as other lipid species. Conclusions: The lipid metabolomic profile varied significantly between CCRCC and control. Further studies are required to confirm the identities of the lipid species contributing to this variation by obtaining structural information using tandem MS (LC-MS/MS).

The lipid metabolome of kidney cancer.

2012 ◽  
Vol 30 (30_suppl) ◽  
pp. 16-16
Author(s):  
Saby George ◽  
Kyoung-Soo Choi ◽  
Jeff C. Miecznikowski ◽  
Roberto Pili ◽  
A. Latif Kazim
Keyword(s):  
Kidney Cancer ◽  
Treatment Strategies ◽  
Charge Ratio ◽  
P Value ◽  
Detailed Knowledge ◽  
Lipid Profiling ◽  
Butyl Ether ◽  
Bonferroni Adjustment ◽  
Lipid Species ◽  
And Control

16 Background: The commonest type of kidney cancer is CCRCC. Kidney cancer is known to accumulate lipids and a detailed knowledge of the lipid species present in these tumors could lead to a better understanding of the underlying aberrant metabolic pathways and suggest possible treatment strategies. We attempt to identify the lipidomic profile of CCRCC using a liquid chromatography MS-based approach (LC-MS). Methods: We utilized 6 fresh frozen representative samples of CCRCC and matching non-tumor areas of kidney from nephrectomy samples. Lipids and other non-polar cellular constituents were extracted from both CCRCC and control tissues by methyl-t-butyl ether /methanol. LC-MS based lipid profiling was performed on a Waters Q-ToF Premier MS coupled with Ultra Performance LC. The peak detection and alignment across all chromatograms were performed using the XCMS software (v 1.14.1, Scripps Center for Metabolomics). Statistical comparisons of the intensities of aligned peaks were performed using the XCMS-built-in Welch's t-test. The XCMS data was converted to log2 ratios (normal/tumor) in order to utilize the paired aspect of this data: each patient’s disease tissue was analyzed in conjunction with corresponding normal tissue. Significance was determined by controlling the family wise error rate (FWER) at 0.05 using a Bonferroni adjustment. All computations were performed using the R statistical software and the “limma” package. Results: The outcome of XCMS was converted to a table that contains fold change, p value and mass to charge ratio (m/z) for each peak, its corresponding retention time, and the integrated peak intensities from all samples. Controlling FWER at 0.05 using a Bonferroni scheme, we found eight statistically significant lipids. Preliminary attempts to identify the analytes included use of METLIN (Scripps Center for Metabolomics) and HMDB (Human metabolome database, Genome Alberta and Genome Canada) databases. The identified metabolites included phosphatidylcholines, cholesterol esters and triglycerides, as well as other lipid species. Conclusions: The lipid metabolomic profile varied significantly between CCRCC and control. Further studies are underway to confirm the identities and significance of the lipid species in detail.

The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique.

2020 ◽  
Vol 3 (11) ◽  
pp. 1100-1104
Author(s):  
Hussein Naeem Aldhaheri ◽  
Ihsan Edan AlSaimary ◽  
Murtadha Mohammed ALMusafer
Keyword(s):  
Personal Information ◽  
Receptor Gene ◽  
Specific Antigen ◽  
Gene Clusters ◽  
Control Group ◽  
P Value ◽  
Group Data ◽  
Elisa Technique ◽  
And Control

      The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.

PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar
Keyword(s):  
Wound Healing ◽  
Caesarean Section ◽  
Amniotic Membrane ◽  
Healing Time ◽  
Cost Of Care ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
And Control ◽  
Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

scholarly journals E MUSCULOSKELETAL DISORDERS (MS PENGARUH WORKPLACE STRETCHING EXERCISE TERHADAP PENURUNAN KELUHAN MUSCULOSKELETAL DISORDERS(MSDs) PADA PEKERJA BATIK TULIS DI KECAMATAN SOKARAJA

2017 ◽  
Vol 9 (02) ◽  
pp. 49
Author(s):  
Siti Harwanti ◽  
Nur Ulfah ◽  
Budi Aji
Keyword(s):  
Musculoskeletal Disorders ◽  
Wilcoxon Test ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Test Analysis ◽  
Result Show ◽  
Post Test ◽  
Equivalent Control ◽  
And Control

Batik maked process especially �mbironi�, is done in sit position. If this position maintained for a long period, that could be cause muscle strain which may lead into musculoskeletal disorders. The research was aim to know the effect of Workplace Stretching Exercise (WSE) to reduced MSDs in hand-made batik workers. The research was quasy experimental by non-equivalent control group design. Subjects were 37 female handmade batik workers used purposive sampling. Data analysis used Friedman test and Wilcoxon test, then for two independent sample used Independent t Test and Mann Whitney test with significancy level at 5% or a = 0,05. Analysis result show that there is no difference in MSDs on experiment and control group after pre-test which had p-value = 0,371 (>0,05). The result of middle-test and post-test p value = 0,000 (<0,05) that there is significant mean difference of MSDs between experiment and control group. Based on the middle-test and post-test analysis result, it could be conclude that there is an effect of WSE to reduce MSDs of handmade batik workers.

scholarly journals Android-Based Education toward the Role of Postpartum Primipara Mothers in Fulfilling the Independent Perinium Care

2021 ◽  
Vol 1 (2) ◽  
pp. 55-67
Author(s):  
Werna Nontji ◽  
Dwi Kartika Sari ◽  
Sitti Maria Ulfa ◽  
Syafruddin Syarif ◽  
Inez Vravty Lestari ◽  
...  
Keyword(s):  
Treatment Group ◽  
Intervention Group ◽  
Wilcoxon Test ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
Post Test ◽  
Android App ◽  
And Control ◽  
Perineal Care

Background: Educating mothers during their postpartum period could potentially help them to overcome some important phases after giving birth. The process of education is evaluated based on the mothers’ knowledge about their independent self-care. Independency is an activity that is started individually and is done based on self-capability. The independency in the postpartum care is not only important to decrease the mother’s mortality and morbidity rate, but it is also crucial to strengthen and improve the post-partum mother’s healthy behavior during the perineal care. Providing education using Android-based application called BUBI Care could be potential to facilitate a more dynamic transfer of knowledge to the postpartum mothers.Aims: To analyze the knowledge, skills, and independence of primipara postpartum mothers in independent perineal care before and after accessing BUBI Care app. Research Method: employing quasi experimental research with pre-test and post-test design with control group design. The sample for this research were 19 pregnant mothers TM III (pregnancy age of ? 38 weeks) on each group. The treatment group was educated using BUBI Care Android app that was conducted at one of Public Health Center. The control group was educated without BUBI Care that was conducted at a Midwife Practice Clinic. The research was conducted on September to October 2020. Study Result: According to the Wilcoxon test, there was a difference in the pre-test knowledge of the treatment and control groups with the similar median of 53 and p-value of 0.666, the treatment group showed their scores improved to 80 on the post-test while the control group stayed at 53 with the p-value of 0.000. It means that BUBI Care app education influences the post-test. On the other hand, the perineal care skill saw a difference between the intervention and control groups. The intervention group had a mean of 70.05 and 56.68 for the control group with the p-value of 0.002 which means that there was an influence from the BUBI Care app education. Additionally, Mann Whitney test showed that the intervention group had a mean score of 78.95, but the control group only had 49.26, the total difference between the two are 29.69 with the p-value of 0.000. It can be concluded that there is a significance in difference in the independency rate from the provision of BUBI Care Android app education.Conclusion:  there is a significance effect on the intervention group in terms of Android based usage.

scholarly journals EFFECT OF AUDIO THERAPY USING AL-QUR'AN MURROTAL ON BEHAVIOR DEVELOPMENT IN CHILDREN WITH AUTISM

2017 ◽  
Vol 3 (5) ◽  
pp. 470-477
Author(s):  
Anjar Astuti ◽  
Suryono Suryono ◽  
Melyana Nurul Widyawati ◽  
Ari Suwondo ◽  
Mardiyono Mardiyono
Keyword(s):  
Neurodevelopmental Disorder ◽  
Alternative Therapy ◽  
Children With Autism ◽  
Simple Random Sampling ◽  
Behavioral Development ◽  
Control Group ◽  
P Value ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Background: Autism is a neurodevelopmental disorder. Audio therapy is considered as one of the complementary therapies to improve the behavior of children with autism.Objective: This study was to analysis the effectiveness of the Al-Qur'an murrotal audio therapy on behavioral development in children with autism.Methods: This study was a true-experimental research using pretest and posttest approach with control group. This research was conducted on November 2016 in the Autism Foundation of Semarang City. There were 30 samples were selected using simple random sampling, with 15 samples assigned in the experiment and control group. Mann Whitney and Wilcoxon tests were used for data analysis in this study.Results: Results showed that there was a significant difference in the mean of behavioral development after given intervention between the experiment group and control group with p-value 0.034 (<0.05). The mean of behavioral development in the experiment group (4.53) was higher than the mean in the control group (3.47).Conclusion: The Al-Qur'an murrotal audio therapy is effective to develop behavior of children with autism. Therefore, it is expected that this audio therapy can be applied an alternative therapy for children with autism.

scholarly journals EFFECT OF BACK EXERCISE TO DECREASE LOW BACK PAIN OF BATIK ARTISANTS IN KAUMAN VILLAGE, SOKARAJA

2019 ◽  
Vol 11 (1) ◽  
pp. 45
Author(s):  
Siti Harwanti ◽  
Budi Aji ◽  
Nur Ulfah
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Treatment Group ◽  
Exercise Therapy ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Low Back ◽  
Control Group Design ◽  
And Control

ABSTRACT Low back pain ( LBP ) is a musculoskeletal disorder that occurs in the lower back which can be caused by various diseases and  activity of the body being less good. One effort to overcome the LBP is back exercise. The research purpose is to find out the effect of back exercise therapy which concern to lowering painful complaint of batik artisants in Kauman village Sokaraja subsdistrict. This research is using Quasi Experiment technique phenomenological non equivalen control group design (pre -post test). Sample taking is using purposive sampling technique. Data analysis use Friedman experiment for treatment group and Repeated Annova experiment for control group. Experiment analysis is different between treatment group and control group with using Mann Whitney experiment. The result of Friedman experiment in treatment group shows that p-value=0,000(p<0,05), it means the therapy those given is effective to lowering painful complaint. The result of Repeated Annova experiment in control group shows that p-value=0,234(p>0,05), it means the painful complaint seems not different in control group. The result of Mann Whitney experiment shows that p-value=0,000(p<0,05) it means there are differences between painful complaint in treatment group and control group after doing back exercise. There is the effect by giving back exercise therapy which concern to lowering the complaint of low back pain for batik artisants in Kauman village Sokaraja subdistrict.   Key words: Handmade Batik, Low back pain, back exercise

scholarly journals Pengaruh Pemberian Tablet Fe Terhadap Hemoglobin Pada Taruni Politeknik Ilmu Pelayaran Semarang

2020 ◽  
Vol 2 (2) ◽  
pp. 52-67
Author(s):  
Anggi Vina Hariyati ◽  
Cahyaningrum ◽  
Adil Zulkarnain
Keyword(s):  
Intervention Group ◽  
Sampling Technique ◽  
Bivariate Analysis ◽  
Control Group ◽  
P Value ◽  
Merchant Marine ◽  
Purposive Sampling ◽  
The Public ◽  
Significant Difference ◽  
And Control

Anemia is one of the public health problems that need more attention of the female because they experience menstruation and they are in the growth phase. From the data of Semarang City Health Office  in 2011, it is shown the incidence of anemia in adolescents aged 10-19 years old are 68.24%. The prevention effort of the case is by measuring hemoglobin and prescribing blood supplement. The objective of the study is to find out the effect of Fe tablets on Hemoglobin in Semarang Merchant Marine Polytechnique students. The objective of the study is to find out the effect of Fe tablets on Hemoglobin in Semarang Merchant Marine Polytechnique students. This research used pre-experimental design with the two group pretest posttest. The population in this Study were all Merchant Marine Polytechnique Semarang at the 4th semester of female students  amounting to 50 with the total respondents 34 respondents taken with purposive sampling technique. The data were collected by performing Hemoglobin examination. The results of the study show that the average Hemoglobin before being prescribed Fe on the intervention and control group was 11.29 g/dl. After being prescribed Fe to the intervention group, it is found the average increased to 13.69 g/dl, while in the control group it is found averaged 11.72 g/dl. Bivariate Analysis uses an independent t test, the value of t is 6.136 with a p-value of   0.000. It shows that the p-value is 0,000 <((0.05), this indicates that there is a significant difference in the decrease in Hemoglobin levels in the population after being prescribed blood supplement between the intervention and control groups in The Semarang Merchant Marine Politechnique Students. It is suggested that the female adolescents to consume more iron-containing foods or consume Fe supplements such as Fe, during menstruation. ABSTRAK  Anemia merupakan salah satu masalah kesehatan masyarakat yang perlu khusus nya pada remaja  putri yang perlu mendapatkan perhatian khusus karena remaja putri mengalami mestruasi tiap bulan dan dalam masa pertumbuhan . Hasil Data Dinas Kesehatan Kota Semarang tahun 2011.  Kejadian anemia pada remaja usia 10-19 sebanyak 68,24%. Untuk menangani masalah tersebut pencegahan yang dilakukan yaitu dengan cara mengukur hemoglobin dan pemberian suplemen tablet tambah darah . Penelitian ini bertujuan untuk melihat Pengaruh Pemberian Tablet Fe Terhadap Hemoglobin Pada Taruni Politeknik Ilmu Pelayaran Semarang. Penelitian ini bertujuan untuk melihat Pengaruh Pemberian Tablet Fe Terhadap Hemoglobin Pada Taruni Politeknik Ilmu Pelayaran Semarang. Jenis penelitian ini menggunakan  pre-eksperimen design dengan rancangan Two group pretest posttest. Populasi dalam penelitian iniadalah seluruh taruni politeknik ilmu pelayaran semarang semester 4 berjumlah 50 taruni dengan jumlah sempel 34 responden dengan tehnik pengambilan sempel purposive sampling. Pengambilan data dengan melakukan pemeriksaan Hemoglobin.  Penelitian menunjukan bahwa rata-rata Hemoglobin sebelum diberikan pada taruni kelompok intervensi  dan  kontrol sama yaitu  11,29 g/dl.  Dan setelah diberikan pada kelompok intervensi  rata-rata meningkat menjadi 13,69 g/dl, sedangkan  kelompok kontrol rata-rata sebesar 11,72 g/dl. Analisis bivariate Menggunakan uji t independen, didapatkan nilai t hitung 6,136 dengan p-value sebesar 0,000. Terlihat bahwa p-value 0,000 <a (0,05), ini menunjukkan bahwa ada perbedaan secara signifikan penurunan (selisih) kadar Hemoglobin b pada taruni sesudah diberikan tablet penambah darah antara kelompok intervensi dan kontrol pada taruni Politeknik Ilmu Pelayaran Semarang.  Hendaknya bagi remaja putri lebih banyak mengomsumsi makanan yang mengandung zat besi atau mengomsumsi suplemen yang mengandung besi seperti Fe terutama saat mestruasi

scholarly journals Effect of Probiotics on Allergic Rhinitis: A Randomized, Controlled, Clinical Trial

2020 ◽  
Vol 9 ◽  
pp. 1918 ◽  
Author(s):  
Mahnaz Sadeghi-Shabestari ◽  
Yalda Jabbari Moghaddam ◽  
Hasan Rezapoor ◽  
Mojataba Sohrabpour
Keyword(s):  
Clinical Trial ◽  
Allergic Rhinitis ◽  
Standard Therapy ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
P Value ◽  
Nasal Discharge ◽  
Oral Corticosteroids ◽  
And Control

Background: Allergic rhinitis (AR) is one of the most common diseases in the world and affects about 10-50% of the general population. Probiotics are live microorganisms that help the normal state of the intestine, and if prescribed correctly, they can stimulate the mucosal immune system to prevent inflammatory symptoms of allergy and atopy. The present study aims to investigate the role of probiotics in the treatment of AR when added to standard therapy as adjuvant agents. Materials and Methods: In this clinical trial study, 28 patients older than 15 years with AR randomly divided into probiotics and control groups. The probiotics group received standard therapy for AR accompanied by probiotic capsules every 12 hours for eight weeks, whereas the control group received standard therapy for AR with placebo capsules as the same protocol. Data were analyzed using SPSS Version 23 (IBM Corporation, Armonk, NY, USA) and, the P-value less than 0.05 was considered statistically significant. Results: In the probiotics group, 14.3% of patients had sneezing at the baseline, which significantly decreased to 4.6% (P<0.01). Also, the necessity for nasal and oral corticosteroids after treatment with probiotics in the probiotics group was less than the control group (P<0.01). Although cough, nasal discharge, conchae hypertrophy, and night sleep disorders reduced after treatment with probiotics, this reduction was not statistically significant between the two groups. Conclusion: Based on the results of this clinical trial, the use of probiotics had no significant effect on the outcome of patients with AR. [GMJ.2020;9:e1918] 

scholarly journals EFFECT OF COMBINATION OF HYPNOBREASTFEEDING AND ACUPRESSURE ON ANXIETY AND WOUND PAIN IN POST-CAESAREAN MOTHERS

2017 ◽  
Vol 3 (5) ◽  
pp. 525-532
Author(s):  
Diah Evawanna Anuhgera ◽  
Tjahjono Kuncoro ◽  
Sri Sumarni ◽  
Mardiyono Mardiyono ◽  
Ari Suwondo
Keyword(s):  
Public Hospital ◽  
Alternative Therapy ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Data ◽  
Before And After ◽  
Wound Pain ◽  
And Control

Background: Post-cesarean mothers often experience anxiety and discomfort due to long-term pain. The combination of hypnobreastfeeding and acupressure is considered to be effective in reducing anxiety and pain levels. Objective: This study aims to examine the effect of combination of hypnobreastfeeding and acupressure on anxiety and pain levels in post-caesarean mothers.Methods: This study was a true experiment with pretest-posttest control group design, conducted in the Ambarawa Public Hospital on 5 November to 9 December 2016. There were 36 participants selected using stratified random sampling, with 18 assigned in the experiment and control group. Data were analyzed using paired t-test and wilcoxon test.Results: There were statistically significant differences of anxiety and pain levels before and after intervention in the experiment and control group with p-value 0.001 (<0.05).Conclusion: The combination of hypnobreastfeeding and acupressure has a significant effect in reducing anxiety and pain levels in post-cesarean mothers. This intervention could be applied as an alternative therapy in treating post-caesarean mothers.

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