Polymorphism of genes of hemostasis system and methionin exchange in patients with gastrointestinal tumors.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e16011-e16011
Author(s):  
Oleg I. Kit ◽  
Liubov Yu Vladimirova ◽  
Tatiana A. Zykova ◽  
Oksana V. Katelnitskaya ◽  
Andrey A. Maslov ◽  
...  
Keyword(s):  
Univariate Analysis ◽  
Cancer Control ◽  
Main Group ◽  
Control Group ◽  
Hemostasis System ◽  
Genotype Frequencies ◽  
Mtrr A66g ◽  
Group 12 ◽  
Hardy Weinberg Equilibrium ◽  
Pai 1

e16011 Background: The purpose of the study was to analyze the rates of polymorphic allelic variants of genes of the hemostasis system and methionin exchange in patients with gastrointestinal cancers (GICs). Methods: The study included 69 patients with histologically verified GICs (main group): gastric cancer (GC) – 17, colon cancer (CC) – 42, other tumors – 10 (pancreatic cancer – 6, liver cancer – 3, gallbladder cancer – 1) and 50 patients without cancer (control group). 12 polymorphic loci were determined in genomic DNA samples by Real-time PCR: F2 (G20210А, rs1799963), F5 (G1691A, rs6025), F7 (G10976A, rs6046), F13 (G226A, rs5985), FGB G(-455)A (rs1800790), ITGA2-α2 (C807T, rs1126643), ITGB3-b (Т1565С, rs5918), PAI-1 4G(-675)5G, rs1799889), MTHFR (С677Т, rs1801133 and A1298C, rs1801131), MTR (А2756G, rs1805087), MTRR (A66G, rs1801394). Results: The ratio of genotype frequencies maintained in the Hardy-Weinberg equilibrium in all gene loci except FGB G(-455)A in GC patients (p = 0.02). The rate of an alternative allele in the F2 gene among patients with GICs was 1.4%, in the control group – 1.0%; F5 – 1.0% and 4.0%; F7 – 13.0% and 11.0%; F13 – 31.9% and 32.0%; FGB – 29.7% and 22.0%; ITGA2 – 37.7% and 38.0%; ITGB3 – 17.4% and 21.0%; PAI-1 – 55.1% and 56.0%, MTHFR (Т) – 26.6% and 31.3%; MTHFR (С) – 33.0% and 27.5%; MTR – 29.8% and 26.3%; MTRR – 51.1% and 57.5%, respectively (p > 0.05). AA homozygotes at the FGB G(-455)A locus were more frequent in the main group, compared to controls: 4.3% vs 4.0%; p = 0.02. No differences in the frequency of alternative alleles of the studied genes were found between patients with GC and CC. GG genotype at the FGB G(-455)A locus was found in GC patients in 29.4%, in controls – in 60.0% (OR = 0.28, 95% CI 0.08-0.91); GA genotype – in 70.6% and 36.0% (OR = 4.27, 95% CI 1.29-14.06), AA genotype – in 0% and 4.0% (p = 0.04, χ2 = 6.34), respectively. Conclusions: The univariate analysis demonstrated that carriage of the GA genotype at the FGB G(-455)A (rs1800790) locus could be found more often in patients with GC.

scholarly journals STUDY OF THE MESOTHERAPY EFFECT WITH EPIPHYSIS PEPTIDES ON THE MOISTURE AND ELASTICITY OF THE SKIN

2017 ◽  
Vol 20 (5) ◽  
pp. 305-310
Author(s):  
Tatiyana N. Korolkova ◽  
S. E Goma
Keyword(s):  
Moisture Content ◽  
Main Group ◽  
Control Group ◽  
Isotonic Solution ◽  
Metabolic Processes ◽  
Skin Changes ◽  
Age Related ◽  
Intradermal Administration ◽  
Group 12 ◽  
Before And After

Introduction. Mesotherapy is a widely used method of preventing external signs of aging in cosmetology. However, drugs that have proven effectiveness are not enough, therefore epiphyseal peptides are of great interest. Purpose: is to study the effect of intradermal administration of peptide epiphysis on the functional para-meters of the skin. Material and methods. There were 38 women in the research devided into 2 comparable groups. The main group (26 persons) was treated by mesotherapy with epiphyseal peptides. Face, neck and decollete zones were treated by the method of “deep nappage” once a week, 5 procedures per course. Isotonic solution of NaCl was similarly injected into the neck and decollete areas of the control group (12 people). The moisture content (Corneometer CM 825, “Courage & Khazaka”, Germany) and the elasticity (Cutometer MPA 580, “Courage & Khazaka”, Germany) of the skin were determined. Functional skin parameters were evaluated before and after treatment (in both groups) and also on the 1st and 6th months after mesotherapy in the main group. Results. Under the influence of epiphysis peptides the moisture content of the skin increases by 10% after treatment (in the control group without changes) and after 1 month, then decreases by 5% in 6 months below the initial values. In the main group R0 and R8 parameters increase immediately after treatment by 21% and 20% (in the control group R0 and R8 decrease by 4-6%), then decrease by 6-7% and by 28%. The R4 parameter increases immediately after treatment by 20-25% (in the control group it decreases by 13%), then decreases by 12% and 24%; F0 and F1 parameteres increase immediately after treatment by 17% and 22% (in the control group they decrease by 4% and 3%), then decrease by 11% and 9%, by 25% and 28%. Conclusion. The revealed dynamics of skin functional indexes may support the stimulating effect of peptide epiphysis on the cellular elements of the dermis (activation of metabolic processes after treatment and improvement of elasticity parameters by 25-28% after 6 months). Mesotherapy with epiphyseal peptides can be recommended as a method of prevention and correction of age-related skin changes.

Features of pregnancy, childbirth and postpartum period in sexually transmitted infections

2020 ◽  
pp. 63-65
Author(s):  
O.І. Krotik ◽  
Keyword(s):  
Pregnant Women ◽  
Sexually Transmitted Infections ◽  
Premature Birth ◽  
Main Group ◽  
Control Group ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Sexually Transmitted ◽  
Placental Dysfunction ◽  
Group 12

The objective: to identify the features of pregnancy, childbirth, the postpartum period in patients with sexually transmitted infections. Materials and methods. A retrospective analysis of 150 pregnancy and childbirth histories was performed: the main group included 100 pregnant women with a history of sexually transmitted infections (STIs) and episodes of manifestations during this pregnancy and 50 pregnant women in the control group without this pathology. The exclusion criteria were pregnant women with sexually transmitted infections whose pregnancies ended in short-term abortions. Results. The threat of abortion was detected in 46% of the main group, against 26% of the control group. Oligohydramnios 13% in the main group against 8% in the control group. Preeclampsia in the main group 12%, against 5% in the control group. FGR in the main group 10% vs. 6% in the control. Placental dysfunction in the main group of 20% vs. 16% in the control. Bacterial vaginosis was 67% in the main group versus 14% in the control group. The risk of miscarriage, premature birth in the main group is 20% compared with the control group of 4%. Premature rupture of membranes was observed in 33% of women in the main group against 16% in the control group. Conclusions. The threat of abortion occurred in women of the main group (46%), which is 1.7 times more often than in the control group (26%). Oligohydramnios was observed in (13%) of the main group, which is 1.6 times more than in the control group (8%). Preeclampsia occurred 2 times more often in the main group (12%) than in the control group (6%). FGR occurred 1.7 times more often in the main group (10%) than in the control group (6%). Placental dysfunction was 1.25 times more common in the main group (20%) than in the control group (16%). A high percentage of bacterial vaginosis (67%) was observed in patients of the main group, which is 4.8 times higher than in the control group (14%). The risk of miscarriage, premature birth in the main group was higher (20%) and was observed 5 times more often than in the control group (4%). Premature rupture of membranes is observed in (33%) women in the main group, which is 2 times higher than in the control group (16%). Keywords: sexually transmitted infections (STIs), pregnancy, childbirth.

scholarly journals Atopic dermatitis in children and adolescents with obesity and overweight: clinical, anthropometric data, psycho-emotional status and predictors of effective treatment

2021 ◽  
pp. 4-14
Author(s):  
O.B. Nemchaninova ◽  
◽  
M.Yu. Dolgikh ◽  
T.B. Reshetnikova ◽  
◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Children And Adolescents ◽  
Treatment Efficacy ◽  
Univariate Analysis ◽  
Main Group ◽  
Overweight And Obesity ◽  
Control Group ◽  
Emotional Status ◽  
Anthropometric Data ◽  
And Gender

The results of a comprehensive analysis of clinical, anthropometric data and an assessment of the psycho-emotional status of 62 children and adolescents (mean age 11.8 ± 3.0 years) with atopic dermatitis and overweight or varying degrees of obesity (the main group) are presented. The control group included 60 patients (mean age 10.1 ± 3.6 years) with atopic dermatitis and body weight corresponding to age and gender standards. In the main group, 45.2% were children and adolescents with overweight, and with first-, second- and third-degree obesity were 25.8, 11.3 and 17.7% respectively. The severe course of dermatosis in the main group was recorded 1.4 times more often than in the control group. With the aid of univariate analysis of the treatment efficacy predictors, it was revealed that weight loss and improvement in psycho-emotional state can significantly increase the treatment efficacy of atopic dermatitis in children and adolescents with overweight and obesity, which indicates the advisability of interaction between a dermatologist, endocrinologist and psychotherapist in the treatment of this category of patients.

scholarly journals Trauma-induced coagulopathy: prospective observation study

2021 ◽  
pp. 29-34
Author(s):  
Mariana Vyshynska
Keyword(s):  
Venous Blood ◽  
Severe Trauma ◽  
Main Group ◽  
Control Group ◽  
Observation Study ◽  
Hemostasis System ◽  
Primary Hemostasis ◽  
Normal Number ◽  
Trauma Induced Coagulopathy ◽  
Thrombotic Complications

Coagulopathy remains the leading cause of illness and death in people with severe trauma. The aim was to study the indicators of vascular-platelet hemostasis in severe trauma and to establish changes in the main pathophysiological mechanisms of primary hemostasis that occur in patients with trauma-induced coagulopathy, compared with almost healthy individuals of the same age. Materials and methods. The study included 44 patients aged 19 to 55 years (36.1 [28.7; 43.2] years). The control group included 20 patients of the therapeutic department without preconditions for changes in the hemostasis system, and the main group - 24 patients with diagnosis of “severe trauma” who were treated in the anesthesiology and intensive care department. Results and discussion. We studied indicators of intravascular platelet activation. Patients had a normal number of platelets in the venous blood, but the level of spherocytes and spheroechinocytes increased. On the 3rd day after the injury, number of platelets in the venous blood was normal, however the level of discocytes decreased, and the level of discochinocytes, spheroechinocytes and the sum of active forms of platelets increased. On the fifth day, the level of discochinocytes and active forms of platelets, significantly higher (p<0.05) among patients of the main group, and spherocytes, spheroechinocytes and platelets involved in the aggregates, were significantly lower than in the control group of patients (p<0.05). Conclusions. Indicators of vascular-platelet hemostasis in patients with polytrauma had significant differences from those of the control group. This may be evidence of activation of the vascular-platelet system of the hemostasis system and may be a prerequisite for late thrombotic complications in patients with polytrauma

ANALYSIS OF MICROBLE LANDSCAPE IN CHILDREN WITH DISEASES

Vestnik ◽  
2021 ◽  
pp. 102-106
Author(s):  
Ж.И. Рысбаева ◽  
А.А. Амзеева ◽  
Х. Азимова
Keyword(s):  
Escherichia Coli ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Main Group ◽  
Pantoea Agglomerans ◽  
Control Group ◽  
Anaerobic Microorganisms ◽  
Actinomyces Naeslundii ◽  
Parvimonas Micra ◽  
Group 12

Выявляемость патогенных и условно- патогенных микроорганизмов было в два раза выше в основной группе (24 изолята), чем в контрольной- 12 изолят. Немаловажное значение имеет, что у детей с гастродуоденальной патологией отмечается симбиоз патогенных анаэробных микроорганизмов: Escherichia coli5,5%, Enterobacter cloacae2,7%, Enterobacter aerogenes2,7%, Pantoea agglomerans2,7%, Pseudomonas putida2,7%, Veillonella spp. 2,7%, Parvimonas micra 2,7%, Actinomyces naeslundii2,7%, Actinomyces meyeri2,7%, Clostridium histolyticum2,7% , которые усугубляют течение заболевания. При этом сравнительная характеристика показало, анаэробных штаммов высеяно в основной группе в 84,6% случаях по сравнению с контрольной - 15,4%. The detectability of pathogenic and opportunistic microorganisms was twice as high in the main group (24 isolates) than in the control group (12 isolates). It is also important that children with gastroduodenal pathology have a symbiosis of pathogenic anaerobic microorganisms: Escherichia coli 5.5%, Enterobacter cloacae 2.7%, Enterobacter aerogenes 2.7%, Pantoea agglomerans 2.7%, Veudomonas putidaon 2.7%. 2.7%, Parvimonas micra 2.7%, Actinomyces naeslundii 2.7%, Actinomyces meyeri 2.7%, Clostridium histolyticum 2.7%, which aggravate the course of the disease. At the same time, the comparative characteristics showed that anaerobic strains were sown in the main group in 84.6% of cases compared to the control group - 15.4%.

scholarly journals Nonpharmacological Strengthening of Fibrinolysis in Complex Therapy of Primary Open-Angle Glaucoma

2020 ◽  
Vol 17 (1) ◽  
pp. 96-104
Author(s):  
S. I. Makogon ◽  
A. P. Momot ◽  
A. S. Makogon ◽  
T. D. Shaternikova ◽  
Y. A. Bryukhanova
Keyword(s):  
Open Angle Glaucoma ◽  
Main Group ◽  
Control Group ◽  
Open Angle ◽  
Complex Therapy ◽  
Ophthalmologic Examination ◽  
And Control ◽  
Pai 1

Purpose: to study the effect of dosed pneumatic vasocompression (DPVK) on the functional parameters of the organ of vision in patients with primary open-angle gluacoma in the complex therapy of this form of pathology.Patients and Methods. The study included 39 patients with stage II POAG, which were randomized into two groups: main and control. All patients received a conservative complex drug therapy. Patients of the main group were additionally conducted DPVK sessions — twice a week (only 4 procedures during the entire course of treatment). All patients underwent standard ophthalmologic examination, hemostasis and fibrinolysis.Results. In the main group of patients, 1 hour after the end of compression, an increase (from baseline) in t-PA level (by median — 1.4 times) was observed while reducing the content of PAI-1 (by median — 2.7 times); improvement of hydrodynamic indicators (improvement of the outflow lightness coefficient, by 19.04 % in OD and 20.0 % in OS, decrease in KB by 24.7 % in OD and by 22.3 % in OS from the initial level) and retinal sensitivity (decrease the number of cattle type 1 in both eyes: 1.70 times on OD and 2.14 times on OS, decrease in type 2 cattle by 2.70 (OD) and 2.30 times (OS). In this regard, an increase sites of normal retina’s photosensitivity in 1.14 times on the OD and 1.19 times on the OS, in contrast to patients in the control group, where no significant changes were found.Conclusion. The obtained preliminary results of the study indicate the prospects for non-pharmacological stimulation of fibrinolysis in the inpatient stage of the addition of conservative therapy in patients with POAG, achieved with a course of dosed pneumatic vasocompression.

scholarly journals THE INFLUNECE OF 4G/5G POLYMORPHISM OF ACTIVATOR INHIBITOR PLASMINOGEN 1 GENE (PAI-1) ON DEVELOPEMENT DEEP VEIN THROMBOSIS

2020 ◽  
Vol 10 (1) ◽  
pp. 51-57
Author(s):  
Maja Pođanin ◽  
◽  
Rifet Terzić ◽  
Aldijana Avdić ◽  
Amela Jusić ◽  
...  
Keyword(s):  
Risk Factors ◽  
Venous Thromboembolism ◽  
Sampling Procedure ◽  
Control Group ◽  
Vein Thrombosis ◽  
Genotype Frequencies ◽  
North Eastern ◽  
External Risk ◽  
Deep Vein ◽  
Pai 1

Analysis of 4G/5G polymorphisms of PAI-1 gene in interaction with other genetic and external risk factors that induce development of venous thromboembolism can be used for risk assessment for development of venous thromboembolism. Research was conducted on 202 examinees of both genders, older than 18, from north-eastern Bosnia. Experimental group included 100 examinees with diagnosed DVT and 101 examinees who until the sampling procedure did not have diagnosed DVT. In DVT group, following genotype frequencies were determined: 27% of examinees had a normal genotype 5G/5G, 68% are heterozygotes and 5% are mutated homozygotes 4G/4G. In the control group, frequencies of 4G/5G polymorphisms of PAI-1 gene were: 42.6% of examinees have 5G/5G genotype, 55.4% are heterozygotes 4G/5G and 2% are 4G/4G genotype. Obtained results support the hypothesis that PAI-1 in interaction with other genetic and external risk factors probably induces the development of venous thromboembolism.

scholarly journals VEGF gene rs35569394 polymorphism in patients with Polycystic Ovary Syndrome

2020 ◽  
Vol 66 (10) ◽  
pp. 1396-1401
Author(s):  
Sheila Silveira Fernandes ◽  
Alessandra Bernadete Trovó de Marqui ◽  
Daniela Reis Fernandes Teles ◽  
Elisabete Aparecida Montovani Rodrigues Resende ◽  
Marco Fábio Prata Lima ◽  
...  
Keyword(s):  
Polycystic Ovary ◽  
Descriptive Analysis ◽  
Univariate Analysis ◽  
Control Group ◽  
Genotypic Frequency ◽  
Vegf Gene ◽  
Gene Insertion ◽  
Pcos Group ◽  
History Of ◽  
Hardy Weinberg Equilibrium

SUMMARY OBJECTIVE: The relationship between the clinicopathological and sociodemographics characteristics of acral melanomas diagnosed at BACKGROUND: This study aimed to investigate the frequency of VEGF gene insertion (I) / deletion (D) polymorphism (rs35569394) in patients with Polycystic Ovarian Syndrome (PCOS) and to compare with a control population to verify its association with the pathology. METHODS: 206 women participated in this study, 103 with PCOS (group of patients) and 103 without the disease (control group). After extraction of genomic DNA from the samples, molecular analysis was performed by Polymerase Chain Reaction (PCR) and electrophoresis in polycrylamide. Descriptive analysis, univariate analysis and logistic regression model were used. Results were presented in odds ratio (OR) and 95% confidence interval (95% CI), considering the significance of p <0.05. RESULTS: There were no statistical differences between patients and controls for allele frequencies (χ2 = 1.16, p = 0.56). The genotypic frequency distribution was in Hardy Weinberg equilibrium for the patients (χ2 = 2.42; p <0.05), but not for the control group (χ2 = 7.26; p <0.05). Regarding risk factors for the syndrome, a history of familial PCOS is more frequent among women with the syndrome. CONCLUSIONS: In the present study, there is no association between VEGF gene I / D polymorphism and PCOS.

scholarly journals MOBP rs616147 Polymorphism and Risk of Amyotrophic Lateral Sclerosis in a Greek Population: A Case-Control Study

Medicina ◽  
2021 ◽  
Vol 57 (12) ◽  
pp. 1337
Author(s):  
Ioannis Liampas ◽  
Vasileios Siokas ◽  
Athina-Maria Aloizou ◽  
Christos Bakirtzis ◽  
Zisis Tsouris ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Case Control Study ◽  
Univariate Analysis ◽  
Case Control ◽  
University Hospital ◽  
Control Group ◽  
Sporadic Als ◽  
Hardy Weinberg Equilibrium ◽  
Control Study ◽  
Lateral Sclerosis

Background and Objectives: To date, only one study has investigated the association between the rs616147 polymorphism of the Myelin-associated Oligodendrocyte Basic Protein (MOBP) locus and Amyotrophic Lateral Sclerosis (ALS). Materials and Methods: A case-control study was performed. Patients with definite sporadic ALS were prospectively and consecutively recruited from the inpatient and outpatient clinics of the Neurology Department of the General University Hospital of Larissa, Central Greece. Community based, age and sex matched healthy individuals with a free personal and family history constituted the control group. Results: A total of 155 patients with definite sporadic ALS and an equal number of healthy controls were genotyped. The power of our sample size was slightly above 80% and MOBP rs616147 was determined to be in Hardy-Weinberg Equilibrium among healthy participants (p = 1.00). According to the univariate analysis, there was no significant relationship between rs616147 and ALS [log-additive OR = 0.85 (0.61, 1.19), over-dominant OR = 0.73 (0.46, 1.15), recessive OR = 1.02 (0.50, 2.09), dominant OR = 0.74 (0.47, 1.16), co-dominant OR1 = 0.71 (0.44, 1.14) and co-dominant OR2 = 0.88 (0.42, 1.84). Additionally, the effect of rs616147 on the age of ALS onset was determined insignificant using both unadjusted and adjusted (sex, site of onset) cox-proportional models. Finally, rs616147 was not related to the site of ALS onset. Conclusions: Our study is the first to report the absence of an association between MOBP rs616147 and ALS among individuals of Greek ancestry. Additional, larger nationwide and multi-ethnic studies are warranted to shed light on the connection between rs616147 and ALS.

Abstract 165: Angiotensinogen M235T Modifies the Relationship between PAI-1 and Renal Blood Flow in Caucasian Hypertensive Individuals

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Qaiser Shafiq ◽  
Bei Sun ◽  
Jonathan S Williams
Keyword(s):  
Blood Flow ◽  
Renal Blood Flow ◽  
Renin Angiotensin System ◽  
Plasminogen Activator Inhibitor ◽  
Plasminogen Activator Inhibitor 1 ◽  
Cardiovascular Damage ◽  
Genotype Frequencies ◽  
Hardy Weinberg Equilibrium ◽  
The Relationship ◽  
Pai 1

Studies have reported an association of angiotensinogen (AGT) gene polymorphisms with hypertension and renal blood flow (RBF). The T allele of rs699 (AGT 235T) is associated with increased levels of angiotensinogen and reduced RBF. Plasminogen activator inhibitor-1 (PAI-1) is an inhibitor of the fibrinolytic pathway. Elevated PAI-1 is associated with increased renin-angiotensin system (RAS) activity and cardiovascular damage. We hypothesized that hypertensive individuals with the TT genotype of AGT 235 would display higher levels of PAI-1. We further hypothesized an inverse relationship would be observed between PAI-1 and RBF, and that the strength of this correlation would be dependent on genotype status. A total of 159 Caucasian hypertensive participants (mean age 49.3y, BMI 27.6) had data available for AGT235, PAI-1 and RBF from the HyperPATH study. RBF was measured by para-aminohippurate clearance method while on a liberal salt diet (>200 mmol Na/day for 1 week). Genotype frequencies were in Hardy-Weinberg Equilibrium (MM 41/MT 94/TT 24). Multivariate regression demonstrated the TT genotype was associated with higher PAI-1 levels compared with MT + MM (41.0±8.1 v. 26.9±7.1ng/ml, p=0.005). Genotype status modified the relationship between PAI-1 and RBF whereby only the TT genotype showed a significant correlation after adjusting for age, BMI and BP (TT pr2=0.22, Beta= -1.66 p=0.001 v. MT+MM pr2=0.05, Beta= -0.82, p=0.21). AGT 235T is associated with elevated PAI-1 and influences the relationship between PAI-1 and RBF in Caucasian hypertensives. This provides mechanistic clues to explain the genetic underpinnings involving AGT polymorphisms and hypertension.

Sign in / Sign up

Export Citation Format

Share Document