scholarly journals Rewarding compounds identified from the medicinal plant Rhodiola rosea

2020 ◽  
Vol 223 (16) ◽  
pp. jeb223982
Author(s):  
Birgit Michels ◽  
Katrin Franke ◽  
Aliće Weiglein ◽  
Haider Sultani ◽  
Bertram Gerber ◽  
...  
Keyword(s):  
Dopamine Neurons ◽  
Rhodiola Rosea ◽  
Rewarding Effect ◽  
Root Extracts ◽  
Pure Compounds ◽  
Chemical Profiles ◽  
Nervous System Function ◽  
Positive Correlations ◽  
Behavioral Impact ◽  
Addictive Potential

ABSTRACTPreparations of Rhodiola rosea root are widely used in traditional medicine. They can increase life span in worms and flies, and have various effects related to nervous system function in different animal species and humans. However, which of the compounds in R. rosea is mediating any one of these effects has remained unknown in most cases. Here, an analysis of the volatile and non-volatile low-molecular-weight constituents of R. rosea root samples was accompanied by an investigation of their behavioral impact on Drosophila melanogaster larvae. Rhodiola rosea root samples have an attractive smell and taste to the larvae, and exert a rewarding effect. This rewarding effect was also observed for R. rosea root extracts, and did not require activity of dopamine neurons that mediate known rewards such as sugar. Based on the chemical profiles of R. rosea root extracts and resultant fractions, a bioactivity-correlation analysis (AcorA) was performed to identify candidate rewarding compounds. This suggested positive correlations for – among related compounds – ferulic acid eicosyl ester (FAE-20) and β-sitosterol glucoside. A validation using these as pure compounds confirmed that the correlations were causal. Their rewarding effects can be observed even at low micromolar concentrations and thus at remarkably lower doses than for any known taste reward in the larva. We discuss whether similar rewarding effects, should they be observed in humans, would indicate a habit-forming or addictive potential.

scholarly journals Antioxidant and Antibacterial Activity of Roseroot (Rhodiola rosea L.) Dry Extracts

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1767 ◽  
Author(s):  
Olga Kosakowska ◽  
Katarzyna Bączek ◽  
Jarosław L. Przybył ◽  
Ewelina Pióro-Jabrucka ◽  
Weronika Czupa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Rhodiola Rosea ◽  
Array Detector ◽  
Chemical Compositions ◽  
Antioxidant Power ◽  
Scavenging Capacity ◽  
Chemical Profiles

Roseroot (Rhodiola rosea L.) belongs to plants revealing adaptogenic properties, which are attributed to the presence of specific phenolic compounds and are reflected mainly as antioxidant activity. The aim of the present study was to determine the antioxidant and antibacterial activity of various products obtained from R. rosea (underground organs as well as their aqueous and ethanolic dry extracts) in relation to the chemical profiles of phenolic and essential oil compounds. The chemical profiles were determined by High-performance Liquid Chromatography with a diode-array detector (HPLC-DAD) and Gas chromatography-mass spectrometry (GC-MS), antioxidant activity by (1,1-Diphenyl-2-picrylhydrazyl) Scavenging Capacity Assay (DPPH), (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) Scavenging Capacity Assay (ABTS) and Ferric Reducing Antioxidant Power Assay (FRAP) and antimicrobial properties were expressed as minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) values following the broth microdilutions method. The results show that the investigated samples differed in terms of their chemical compositions and biological activities. The extracts were more abundant in phenolic compounds (salidroside, tyrosol, and rosavin derivatives) in comparison to dried underground organs. The content of the determined phenolics in the analyzed extracts was affected by the solvent used for extraction. The ethanolic extract was characterized by the highest content of these substances in comparison to the aqueous one and the dried raw material, especially with regard to rosavin (969.71 mg/100 g). In parallel, this extract showed the strongest antioxidant and antibacterial activity. However, dried R. rosea underground organs also revealed strong antibacterial effects against, for example, Staphylococcus strains.

Prospective neurobiological effects of the aerial and root extracts and some pure compounds of randomly selectedScorzoneraspecies

2014 ◽  
Vol 52 (7) ◽  
pp. 873-882 ◽  
Author(s):  
F. Sezer Senol ◽  
Ozlem Bahadir Acikara ◽  
Gulcin Saltan Citoglu ◽  
Ilkay Erdogan Orhan ◽  
Stefano Dall’ Acqua ◽  
...  
Keyword(s):  
Root Extracts ◽  
Pure Compounds

scholarly journals Chemovariation and antibacterial activity of extracts and isolated compounds from species of Ixora and Greenea (Ixoroideae, Rubiaceae)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6893 ◽  
Author(s):  
Raveevatoo Buathong ◽  
Voradol Chamchumroon ◽  
Johann Schinnerl ◽  
Markus Bacher ◽  
Wichai Santimaleeworagun ◽  
...  
Keyword(s):  
Stem Bark ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Root Bark ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Plant Parts ◽  
Pure Compounds ◽  
Chemical Profiles

Background A large number of secondary metabolites can be obtained from plants used for traditional medicine in two related genera (Ixora and Greenea) in the subfamily Ixoroideae (Rubiaceae), but there are only a few detailed studies on their bioactivities. Therefore, the main goals of this study were to determine the antibacterial activities of lipophilic extracts from plants of some Ixora and Greenea species native to Thailand, and to isolate some pure compounds from those extracts. Moreover, we compared the occurrence of compounds in different plant parts of samples from different habitats to better understand their variation. Methods A total of 56 lipophilic extracts were obtained from the leaves, stem bark, and root bark of eight Ixora and two Greenea species collected at various locations in Thailand. Isolated compounds were identified using nuclear magnetic resonance. Antimicrobial activities were evaluated against four Gram-positive and nine Gram-negative human pathogenic bacterial strains. Results Extracts from I. javanica, I. nigricans, I. brunonis, and G. montana, along with isolated scopoletin, exhibited antibacterial activities against Gram-positive methicillin-resistant Staphylococcus aureus ATCC 43300, with minimum inhibitory concentration values ranging from 64 to 256 µg/mL. The occurrence of scopoletin, isofraxidin, and geniposidic acid in lipophilic extracts showed some variation among different plant parts and species. Conclusions Lipophilic extracts of Ixora and Greenea species have the potential to be developed as anti-Gram-positive agents, in particular to counter infections of methicillin-resistant S. aureus strains. The chemical profiles showed differences between floristic regions but similarity within the same plant parts.

scholarly journals Investigation of key chemical species from durian peduncles and their correlations with durian maturity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Preeyarad Charoensumran ◽  
Kornkanya Pratumyot ◽  
Tirayut Vilaivan ◽  
Thanit Praneenararat
Keyword(s):  
Chemical Species ◽  
Abundant Species ◽  
High Price ◽  
Maturity Stage ◽  
Spectroscopic Techniques ◽  
Predictive Tool ◽  
Chemical Profiles ◽  
Positive Correlations ◽  
The Relationship ◽  
Non Destructive

AbstractThe popularity and high price of durian make quality control in terms of ripeness very important, which in turn depends heavily on harvesting at an appropriate maturity stage. To date, reports on data-driven methods for maturity prediction are scarce, with many rather focusing on ripeness prediction. Herein, we report the first disclosure of key molecular markers in the liquid extract of durian peduncle that can be a predictive tool for maturity. Multiple chromatographic and spectroscopic techniques including TLC, HPLC, PS-MS, LC–MS/MS, and NMR, were used to characterize chemical profiles of the aqueous extracts from peduncles at different ages. Four compounds that show positive correlations with maturity were identified as sucrose, asparagine, arginine, and pipecolic acid, with asparagine as the most abundant species. This finding paves the way for more research of high impact such as the relationship between biochemical reactions in peduncle and pulp, and the development of accurate and non-destructive sensors for maturity prediction.

scholarly journals Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum officinale L.) Root Extracts Obtained by Different Extraction Techniques

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 837 ◽  
Author(s):  
Nataša Nastić ◽  
Isabel Borrás-Linares ◽  
Jesús Lozano-Sánchez ◽  
Jaroslava Švarc-Gajić ◽  
Antonio Segura-Carretero
Keyword(s):  
Fatty Acids ◽  
High Performance ◽  
Polar Compounds ◽  
Extraction Techniques ◽  
Root Extracts ◽  
Flight Mass Spectrometry ◽  
Solvent Type ◽  
Chemical Profiles ◽  
Solid Liquid ◽  
Phytochemical Profiles

In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extraction parameters on phytochemical profiles of S. officinale root was investigated for all applied techniques. PLE and maceration, using alcohol-based solvents (aqueous methanol or ethanol), were shown to be more efficient in the recovery of more polar compounds. Greater numbers of phenolics were best extracted by PLE using 85% EtOH at 63 °C. The use of SFE and 100% acetone for 30 min enabled good recoveries of nonpolar compounds. SFE using 15% EtOH as a cosolvent at 150 bar produced the best recoveries of a significant number of fatty acids. The main compositional differences between extracts obtained by different extraction techniques were assigned to the solvent type. Hence, these results provided comprehensive approaches for treating comfrey root enriched in different phytochemicals, thereby enhancing its bioaccessibility.

High-voltage Electron Microscopy of astroglial cell whole mounts

1986 ◽  
Vol 44 ◽  
pp. 316-317
Author(s):  
J.N. Turner ◽  
W.G. Shain ◽  
V. Madelian ◽  
R.A. Grassucci ◽  
D.L. Forman
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Time Lapse ◽  
Astroglial Cells ◽  
High Voltage Electron Microscopy ◽  
Rat Glioma ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Nervous System Function ◽  
Beta Adrenergic Agonist

Homogeneous cultures of astroglial cells have proved useful for studying biochemical, pharmacological, and toxicological responses of astrocytes to effectors of central nervous system function. LRM 55 astroglial cells, which were derived from a rat glioma and maintained in continuous culture, exhibit a number of astrocyte properties (1-3). Stimulation of LRM 55s and astrocytes in primary cell cultures with the beta-adrenergic agonist isoproterenol results in rapid changes of morphology. Studies with time lapse video light microscopy (VLM) and high-voltage electron microscopy (HVEM) have been correlated to changes in intracellular levels of c-AMP. This report emphasizes the HVEM results.

Two-Point Discrimination in the Use of Upper Extremity Nerve Function in the AMA Guides

2015 ◽  
Vol 20 (1) ◽  
pp. 9-11
Author(s):  
Richard Katz
Keyword(s):  
Case Report ◽  
Sensory Function ◽  
Sixth Edition ◽  
Steel Manufacturing ◽  
Nerve Recovery ◽  
Nervous System Function ◽  
Tunnel Syndrome ◽  
Nocturnal Awakening ◽  
Subjective Reports ◽  
Obese Male

Abstract This article presents a case report regarding a 34-year-old obese male who works as a chipper and grinder at a steel manufacturing mill and uses high-frequency vibratory power tools. He presents with typical complaints of carpal tunnel syndrome, including numbness in all five digits, wrist pain, nocturnal awakening/numbness, and others. Two-point discrimination (2PD) using a caliper was tested in the digits of the upper extremities and was 5 mm throughout. 2PD first appeared in the AMA Guides to the Evaluation of Permanent Impairment (AMA Guides), Fifth Edition, and the Sixth Edition states, “sensory deficits can be challenging to grade, since the clinical examination is based on subjective reports by the patient. Grading is based on the results of sensibility testing and two-point discrimination, to improve inter-rater reliability.” The discussion of “sensibility” involves a concept of sensory perception that is more appropriate in surgery literature than neurological literature, and the discussion of the case report in this article reflects the recent literature regarding 2PD as a measure of sensibility. The authors report that sensibility is not a simple recovery of sensory function following nerve injury but rather is a complex interaction between nerve recovery and modulation of central nervous system function in spinal cord, subcortical, and cortical structures. The authors ask if the value of 2PD in the clinical assessment of impairment has been overrated within the AMA Guides, as was range of motion in the assessment of spine impairment.

A Test of Three Sadism Measures

2020 ◽  
Vol 41 (4) ◽  
pp. 219-227 ◽  
Author(s):  
Bojana M. Dinić ◽  
Tara Bulut Allred ◽  
Boban Petrović ◽  
Anja Wertag
Keyword(s):  
Item Response ◽  
Social Groups ◽  
Similarity Index ◽  
Dark Triad ◽  
Hierarchical Regression ◽  
Fit Indices ◽  
Profile Similarity ◽  
Positive Attitudes ◽  
Confirmatory Factor ◽  
Positive Correlations

Abstract. The aim of this study was to evaluate psychometric properties of three sadism scales: Short Sadistic Impulse Scale (SSIS), Varieties of Sadistic Tendencies (VAST, which measures direct and vicarious sadism), and Assessment of Sadistic Personality (ASP). Sample included 443 participants (50.1% men) from the general population. Reliability based on internal consistency of all scales was good, and results of Confirmatory Factor Analysis (CFA) showed that all three scales had acceptable fit indices for the proposed structure. Results of Item Response Theory (IRT) analysis showed that all three scales had higher measurement precision (information) in above-average scores. Validity of the scales was supported through moderate to high positive correlations with the Dark Triad traits, especially psychopathy, as well as positive correlations with aggressiveness and negative with Honesty-Humility. Moreover, results of hierarchical regression analysis showed that all three measures of direct, but not vicarious sadism, contributed significantly above and beyond other Dark Triad traits to the prediction of increased positive attitudes toward dangerous social groups. The profile similarity index showed that the SSIS and the ASP were highly overlapping, while vicarious sadism seems distinct from other sadism scales.

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