An improved method of diet preparation for toxicological feeding experiments

1984 ◽  
Vol 18 (3) ◽  
pp. 271-274 ◽  
Author(s):  
R. E. Morrissey ◽  
W. P. Norred
Keyword(s):  
Dietary Treatment ◽  
Feeding Preference ◽  
Feed Consumption ◽  
Distilled Water ◽  
Feed Conversion ◽  
Preparation Time ◽  
Treatment Groups ◽  
Feeding Experiments ◽  
Freeze Dried ◽  
Animal Feeding

Groups of 30 rats each were fed 1 of 4 diets to determine feeding preference for freeze-dried meal compared to dry meal, and to determine if 3% agar is a desirable component of freeze-dried meal. The diets were: dry meal, meal+distilled water(freeze-dried), meal+3% agar in distilled water(freeze-dried), and meal+3% agar in distilled water(gel). Diet preparation, and the method for introducing a toxin in a toxicological study, is described. Feed consumption, bodyweight, and observational data were collected. Rats ate freeze-dried meal in amounts comparable to dry meal. There were no significant variations in feed conversion ratios among the dietary treatment groups. Agar is not required for freeze-dried diets, and we recommend that it be omitted to save resources and preparation time. Freeze-dried diets have a longer storage and cage life, thus the frequency of diet preparation, animal feeding and dean-up arc reduced. For studies involving a toxin, these factors would reduce the potential for accidental exposure of personnel to potentially harmful materials.

scholarly journals Effect of supplementation of vitamin E and selenium from Lolyvit-ES on the performance of broiler

2018 ◽  
Vol 3 (4) ◽  
pp. 521-528
Author(s):  
Mohammad Salahuddin ◽  
Zahirul Haque Khandaker ◽  
Mohammad Mohiuddin ◽  
Md Mostain Billah
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Body Weight Gain ◽  
Dietary Treatment ◽  
Experimental Period ◽  
The Body ◽  
Feed Consumption ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Treatment Groups

An experiment was conducted in the poultry rearing unit of Sahjalal Animal Nutrition Field Laboratory, Bangladesh Agricultural University, Mymensingh for a period of 30 days by using 108 one week aged straight run Starbro broiler chicks to find out the effect of vitamin E and selenium as feed supplement for the performance of broiler. Chicks were distributed into 4 dietary treatment groups each having three replications (R1, R2, R3) and each replication contains 9 chicks. Dietary treatment groups were T0 (Control i.e. without supplemental vitamin E and Selenium), T1 (Control + 50 mg vitamin E 0.11 mg selenium per kg diet), T2 (Control + 100 mg vitamin and 0.22 mg Selenium per kg diet and T3 (Control + 150 mg vitamin E and 0.33 mg Selenium per kg diet). At end of the feeding trail the body weight of birds were 1238.28, 1231.85, 1272.59, and 1218.33 gm/bird in treatment groups T0, T1, T2 and T3 respectively which differ significantly (p<0.05) where highest body weight observed in T2 group . Body weight gain was highest in T2 (control+100mg vitamin E and 0.22 mg Selenium per kg diet) and lowest in T0 (control). Total Feed consumption during the experimental period was 2480.30, 2523.80, 2564.56 and 2555.39 g for T0, T1, T2 and T3 respectively. At 2nd and 3rd week of age, weekly feed conversion ratio were significantly (p<0.05) higher in T2 group than the other groups including control. Meat yield parameter of broilers showed significant (P<0.05) differences in dressing weight, breast weight and shank weight. Based on the above findings, the result suggest that supplementation of Vitamin E 100 mg and 0.22 mg Selenium per kg diet from ‘‘Lolyvit-ES’’ as a source of Vitamin E and Selenium may be used in broiler rations.Asian J. Med. Biol. Res. December 2017, 3(4): 521-528

scholarly journals Effects of the Ratio of Dietary Fish Meal to Soybean Meal on the Performance of Broiler Chicks

2021 ◽  
Vol 4 (1) ◽  
pp. 47-53
Author(s):  
Nwe Nwe Htin ◽  
May Phyo Chit
Keyword(s):  
Body Weight ◽  
Soybean Meal ◽  
Fish Meal ◽  
Control Diet ◽  
Point Of View ◽  
Feed Consumption ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Dietary Fish

A completely randomized design (CRD) was used with a total of 160 day-old commercial broiler chicks which were randomly assigned to four treatment groups with five replicates into 20 pens and brooded. Feed and water were provided ad libitum. From day old chick to 21 day, the broiler chicks were fed Diet 1 (5% fish meal and 25% soybean meal, 1: 5), Diet 2 (3% fish meal and 30% soybean meal, 1: 10) was served as control diet, Diet 3 (2% fish meal and 30% soybean meal, 1: 15) and Diet 4 (1.5% fish meal and 30% soybean meal, 1: 20). From day 22 onwards, the broiler chicks were fed finisher diet, Diet 1 (5% fish meal and 25% soybean meal, 1: 5), Diet 2 (2.6% fish meal and 26% soybean meal, 1: 10, serve as control diet, Diet 3 (1.8% fish meal and 27% soybean meal, 1: 15) and Diet 4 (1.3% fish meal and 26% soybean meal, 1: 20). Weekly performances and mortality are measured. By feeding FS5 diet (5% fish meal and 25% soybean meal), the heavier body weight, increased feed consumption and feed conversion ratio of broiler chicks was observed when compared to that of other treatment groups. Conclusively, it is obvious that the ratio of dietary fish meal to soybean meal 1: 5 (FS5) improved body weight but the lower feed efficiency of this diet should be considered in economical point of view.

scholarly journals Bıldırcın Rasyonlarında Yonca Unu ve Enzim Kullanımının Performans, Karkas ve İnce Bağırsak Parametrelerine Etkisi

2020 ◽  
Vol 8 (6) ◽  
pp. 1353-1358
Author(s):  
Mahmut Mutlu ◽  
Alpönder Yıldız
Keyword(s):  
Dietary Treatment ◽  
Conversion Ratio ◽  
The Body ◽  
Intestinal Development ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Japanese Quails ◽  
Carcass Parameters ◽  
Alfalfa Meal ◽  
Different Levels

This study was carried out to determine the influence of different levels of alfalfa meal and the addition of enzyme on performance, carcass and intestinal parameters in growing quails. Japanese quails (n=240), 1 days of age, were divided into 6 dietary treatment groups and the experiment lasted for 5 weeks. In each experiment group there were 4 replicates, and in each replicates there were 10 chicks. Six diets, arranged a factorial design with three alfalfa meal levels (0, 2.5 and 5.0 %) and two levels of added enzyme (0.0 and 1.0 g/kg) were used. The treatments did not effect on the body weight, feed intake and feed conversion ratio. However, relative liver, leg+back, intestine weights and crypt depth were significantly increased, and relative heart and breast weights and villus width decreased by dietary alfalfa meal levels. The addition of enzyme to diets containing alfalfa meal did not affect the performance and carcass parameters, but reduced the intestinal histomorphology parameters in quails. According to the results obtained from the experiment, it can be said that the use of alfalfa meal in growing quail diets did not affect the performance, but the use of alfalfa meal at the level of 2.5% in the diet promotes intestinal development.

scholarly journals Administration of Saccharomyces boulardii mafic-1701 improves feed conversion ratio, promotes antioxidant capacity, alleviates intestinal inflammation and modulates gut microbiota in weaned piglets

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Wenxiu Zhang ◽  
Chengling Bao ◽  
Jian Wang ◽  
Jianjun Zang ◽  
Yunhe Cao
Keyword(s):  
Intestinal Inflammation ◽  
Animal Health ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Saccharomyces Boulardii ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Weaned Piglets ◽  
Treatment Groups

Abstract Background Probiotics are used as a means to improve animal health and intestinal development. Saccharomyces boulardii is a well-known probiotic; however, few studies have examined the effects of S. boulardii on weaned piglet performance. Therefore, this 28-day study compared the effects of S. boulardii mafic-1701 and aureomycin in diets for weaned piglets on growth performance, antioxidant parameters, inflammation and intestinal microbiota. One hundred and eight piglets, weaned at 28 d of age (8.5 ± 1.1 kg), were randomly divided into the three dietary treatment groups with six pens and six piglets per pen (half male and half female). The dietary treatment groups were as follows: 1) basal diet (CON); 2) basal diet supplemented with 75 mg/kg aureomycin (ANT); 3) basal diet supplemented with 1 × 108 CFU/kg S. boulardii mafic-1701 (SB). Results Compared to CON group, SB group had higher feed efficiency (P < 0.05) in the last 14 d and lower diarrhea rate (P <  0.05) over the entire 28 d. Total superoxide dismutase in serum was markedly increased in SB group (P < 0.05). Moreover, compared with CON group, SB group decreased the levels of pro-inflammatory cytokines interleukin-6 (P <  0.01) and Tumor necrosis factor-α (P < 0.05) in jejunum. Supplementation of S. boulardii mafic-1701 increased the abundance of Ruminococcaceae_UCG_009 and Turicibacter (P < 0.05), whereas the abundance of unclassified_Clostridiaceae_4 was decreased (P < 0.05). Furthermore, S. boulardii mafic-1701 administration increased cecal concentration of microbial metabolites, isobutyrate and valerate (P < 0.05). Conclusions The improvement in feed conversion ratio, reduction in diarrhea rate in weaned piglets provided diets supplemented with S. boulardii mafic-1701 may be associated with enhanced antioxidant activity, anti-inflammatory responses and improved intestinal microbial ecology.

scholarly journals Yaz Koşullarında Gece-Gündüz Yemlemesi ve Aydınlatmanın Etlik Piliçlerin Performansı Üzerine Etkileri

2018 ◽  
Vol 6 (1) ◽  
pp. 91 ◽  
Author(s):  
Mesut Karaman ◽  
Mehmet Öcal
Keyword(s):  
Broiler Chickens ◽  
Live Weight ◽  
Summer Season ◽  
Performance Criteria ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Light Regimen ◽  
Night Feeding ◽  
Ad Libitum

This study, aimed to determine the effects of different light regimen and day/night feeding on some performance criteria of 1104 broiler chickens during summer season. At the beginning of second week they were separated according to their sexes, and experiment had 2 × 6 replications. The 1st group, as control, received continuous lighting (daylight + lightening during night) and fed ad-libitum. The 2nd group was treated with continuous lighting too, but they had no access to feed between10:00 and 16:00 o’clock during the last three weeks. The 3rd group was also exposed to continuous lighting but had no access to feed between10:00 am and 16:00 pm. Fourth group received continuous lighting but had no access to feed between 24:00 pm and 06:00 am. Fifth group received continuous lighting, but they were not fed between 24:00 pm and 06:00 am during the last three weeks of experimental process. Sixth group had no access to feed box during 24:00 pm and 06:00 am, when they had not been exposed to light as well. There were no notable differences in live weight gain of male and female birds for all treatment groups, and similarly differences among feed consumption and feed conversion ratios were not remarkable statistically regardless of the sexes of chickens. There were significant differences in hot-cold carcass yields of the 3rd and 6th treatment groups for male birds and 6th treatment group for female birds. The differences for carcass weights of male birds were statistically significant and 3rd and 6th treatments groups were tend to be relatively higher than the other treatment groups. The differences among treatment groups for carcass weights of female birds, however, were not significant and similarly the effects of the treatments on the mortality rate was found to be in significant for same birds. Lightning (24h) and feeding ad - libitum affected the performance of the birds adversely, whilst restricted feeding and lightning process reduced the yield-loss the animals during the summer season.

scholarly journals Comparative Assessment of Growth Performance and Nutrients Utilization of African Catfish (Clarias gariepinus, Burchell 1882) Fed Chicken Offal and Shrimp-Based Diets

2019 ◽  
pp. 1-11
Author(s):  
Elvis Monfung Ayim ◽  
Ettah Akpang Ivon ◽  
Raymond Odey Ajang ◽  
Akaninyene Paul Joseph
Keyword(s):  
Growth Rate ◽  
Growth Performance ◽  
Juvenile Fish ◽  
Clarias Gariepinus ◽  
Feed Consumption ◽  
African Catfish ◽  
Fish Feed ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Fish Group

A comparative study on the growth performance of African catfish (Clarias gariepinus) fed shrimp based diet (SBD), chicken offal based diet (COBD) and coppens feed was carried-out for 22 weeks in concrete tanks measuring 3.5 x 1.7 x 1.5 m3. Triplicate groups of 40 juveniles with average length of 9.15 ± 0.17cm and weight of 20.00 ± 2.58g were stored in each tank. A total of 360 juveniles were used throughout the study. Fish were fed daily at 3% of their body weight through-out the duration of the experiment. The nutrient composition of the 3 nutrients differed significantly at p<0.05. Weight gain (WG) (g) was highest (17189.33 ± 506.61g) in fish fed diet A (Coppens feed), followed by 15045.33±202.42 g (diet B) (SBD) and least (14357.33 ± 108.25 g) (diet C) (COBD). The Growth rate (GR) was highest in fish fed with diet A (111.62 ± 3.29 g/day), followed by 97.69±1.31 g/day (diet B), and least (93.23±0.70 g/day) in diet C. Specific growth rate (SGR) was highest in juveniles fed diet A (2.02 ± 0.02 %/day), followed by 1.94±0.01 %/day for diet B, and least (1.91±0.01 %/day) for diet C. Mean growth rate (MGR) was highest in juveniles fed diet A (11.88 ± 0.29 mg/day), followed by 11.74±0.15 mg/day for diet B, and least (11.69±0.09 mg/day) in fish fed diet C. The WG, GR and SGR varied significantly between the juvenile fish group fed the 3 diets at p<0.05, while MGR did not vary significantly between fish group fed the 3 diets at p>0.05. Fish fed diet A had a higher feed consumption (FC) (41650.00 ± 315.34 g/kg), than that fed diet B (39034.24 ± 86.34 g/kg) and then diet C (38276.00 ± 342.97 g/kg). Juveniles fed Coppens feed did better, with a feed conversion ratio (FCR) of 2.42 ± 0.06, followed by 2.59±0.04 for diet B and 2.67 ± 0.03 for fish fed diet C. Feed conversion efficiency (FCE) was higher in fish fed diet A (41.26±0.94 %), followed by 38.54 ± 0.54 % (diet B) and least (37.52 ± 0.51 %) (diet C). The FC and FCR varied significantly between the fish fed the 3 diets at p<0.05, while FCE was not significantly different between the 3 treatment groups at p>0.05. The water parameters of the culture water varied significantly between each treatment groups (p<0.05), and were within the rage suitable for normal growth of fish. Though coppens feed yielded better growth performance and feed utilisation, the use of COBD and SBD is recommended for fish farmers in Nigeria. More researches should be carried out on using chicken offals and shrimps in fish feed formulation.

scholarly journals Penampilan Ayam Pedaging yang Mengkonsumsi Pakan Mengandung Tepung Kulit Nanas Disuplementasi dengan Yoghurt

2013 ◽  
Vol 13 (2) ◽  
pp. 15-20 ◽  
Author(s):  
Nurhayati Nurhayati
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Pineapple Peel

Broiler chicken performance feed ration containing pineapple peel meal and supplemented by yoghurtABSTRACT. An experiment was conducted to determine the broiler performance feed ration contained pineapple peel meal supplemented by yoghurt. A hundred broiler chickens 2 days old were used in this study those divided into 4 treatment groups and kept for 4 weeks. The treatments were level of pineapple peel meal (PPM) into the ration; P0 = ration without PPM, P1 = ration contained 5 % of PPM, P2 = ration contained 10 % of PPM and P3 = ration contained 15 % of PPM. To neutralize high crude fiber content in the ration, chickens were supplemented by yoghurt consisted 3,3 x 108 CFU/ml of Lactobacillus bulgaricus and Streptococcus thermophilus as much as 3 ml/kg body weight. Yoghurt was offered in the morning before feeding. Each treatment was replicated 5 times. The experiment was assigned into Completely Randomized Design with 4 treatments and 5 replications. Parameters were feed consumption, water consumption, body weight gain and feed conversion ratio. Data were analyzed by Analysis of variance (ANOVA) and significantly effect was tested by Duncan’s Multiple Range Test. Results of this study showed that there was no significant difference (P0.05) among 0, 5, and 10 % the treatment groups on feed consumption, water consumption, body weight gain and feed conversion ratio. Offered 15 % of pineapple peel to the chickens did not significantly (P0.05) increased feed consumption and conversion ratio but did not significantly (P0.05) decreased body weight gain.  It is concluded that pineapple peel meal might be mixed into the broiler chicken ration up to 10 % without any significant adverse effect  on performance when they were supplemented by yoghurt as much as 3 ml/kg of body weight.

scholarly journals The Effect of Clove Oil Supplementation in Laying Hen Diets on Performance, Egg Quality, Some Blood Parameters, and Yolk TBARS

2021 ◽  
Vol 9 (12) ◽  
pp. 2213-2218
Author(s):  
Melek Şehitoğlu ◽  
Hatice Kaya
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Live Weight ◽  
Blood Parameters ◽  
Feed Consumption ◽  
Control Group ◽  
Clove Oil ◽  
Feed Conversion ◽  
Treatment Groups

In this study, it was investigated the effect of clove oil supplementation at increasing levels into laying hens’ diets on performance, egg quality traits, some blood parameters and yolk TBARS (Thiobarbituric Acid Reagent) values. For this purpose 96 Lohman white laying hens, 28 weeks of age, were divided into four treatment groups. Control group was fed with basal diet (C) and treatment groups were fed with diets formed by addition of clove oil at 50 ppm (CO1), 100 ppm (CO2) and 150 ppm (CO3), respectively. During the trial, feed and water were given as ad-libitum, and poultry house was illuminated for 17 hours. Experiment lasted for 13 weeks. Addition clove oil at increasing rates into diet did not affect the live weight. The data analysed as polynomial showed that supplementation of clove oil into layer diet linearly improved feed conversion ratio and increased the egg production. But, daily feed consumption, egg weight, damaged egg ratio and egg quality parameters were not affected by treatments. Serum parameters such as triglyceride, glucose, aspartate aminotransferase, alanine aminotransferase and calcium were not affected by the clove oil supplementation. TBARS values in C, CO1, and CO2 were found higher than the CO3 group fed with diet including 150 ppm clove oil. In conclusion, clove oil at 150 ppm level in diets of laying hens could be used due to extend the egg shelf life and to decline serum cholesterol content.

Broiler breeder egg production and quality are affected by timing of increased dietary Ca relative to photostimulation

2004 ◽  
Vol 84 (3) ◽  
pp. 411-420
Author(s):  
A. Petruk and D. R. Korver
Keyword(s):  
Body Weight ◽  
Egg Production ◽  
Dietary Treatment ◽  
Feed Consumption ◽  
Optimal Time ◽  
Shell Weight ◽  
Egg Weight ◽  
Broiler Breeder ◽  
Treatment Groups ◽  
Ash Weight

Ross 508 broiler breeder pullets (n = 400) were reared in floor pens in a light-tight facility with feed allocation based on breeder-recommended body weight targets. At 17 wk, 250 pullets within 15% of the mean weight were randomly assigned to individual cages in one of two environmentally controlled environments: 18°C (exp. 1, n = 125) or 28°C (exp. 2, n = 125). The pullets were photostimulated at 20 wk of age. At 2-wk intervals from 18 to 26 wk, successive groups of hens (n = 25 per environment) were switched from a 0.88% Ca grower diet to a 2.96% Ca breeder diet. Dietary treatment names were based on the time (in weeks) relative to photostimulation that the diet was changed from the grower to the breeder diet (PS – 2, PS, PS + 2, PS+4, and PS + 6, respectively). The hens were killed at 31 wk of age and one leg from each hen was taken for bone mineral analysis. The birds in both experiments maintained target body weights as recommended by the primary breeder; however, the hens in exp. 2 did not consume the allotted feed for the final 2 wk of the experiment. Dietary treatment did not affect feed consumption or body weight. Overall percent hen day production was not affected by dietary treatment in either experiment. In exp. 1, hens in the PS + 4 and PS + 6 treatments produced larger eggs and hens in the PS + 4 treatment produced eggs with greater shell weights. Shell weight as a percentage of egg weight was significantly lower from the PS + 6 hens than the other dietary treatment groups. The PS + 4 treatment resulted in the highest bone Ca as a percentage of ash weight, indicating that changing the diet 4 wk after photostimulation was the optimal time to increase bone Ca reserves in that environment. In exp. 2, the PS + 6 treatment had the highest egg weight whereas birds in the PS and PS + 2 treatment groups had the highest shell weights. Percent shell was the lowest in the PS + 6 and PS + 4 treatment groups overall, although it was only significantly different until 27.4 wk of age. Ca as a percentage of bone weight was lowest in the PS + 2 and PS + 6 treatment groups and Ca as a percentage of ash was lowest in the PS + 2 treatment group. Overall, the results suggest that improper timing of increasing dietary Ca either before or too long after photostimulation decreased eggshell quality and bone characteristics. Elevated environmental temperature appeared to exacerbate these effects; therefore, the change from a low-Ca grower to a high-Ca breeder diet must be made with greater precision in hot climates. Key words: Broiler breeder, photostimulation, dietary calcium, calcium metabolism, heat stress

scholarly journals Effect of Nanosilica and Bentonite as Mycotoxins Adsorbent Agent in Broiler Chickens’ Diet on Growth Performance and Hepatic Histopathology

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2129
Author(s):  
Abdallah A. Ghazalah ◽  
Mamduh O. Abd-Elsamee ◽  
Kout Elkloub M. E. Moustafa ◽  
Mohamed Abdelrazik Khattab ◽  
Abd-Elrahim A. A. Rehan
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Control Diet ◽  
Dietary Treatment ◽  
Control Group ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Four Levels ◽  
Hepatic Histopathology

Mycotoxins are toxic secondary metabolites produced by different strains of fungi, such as aspergillus, fusarium, and penicillium that can contaminate feed ingredients or the entire feed of poultry and animals. Mycotoxins can cause many serious complications to both humans and animals due to carcinogenic, mutagenic, and immunosuppressive disorders. Therefore, the present experiment aims to investigate the effect of broiler chickens’ diets supplemented with different levels of nanosilica (NS) as an adsorbent agent of mycotoxins on their growth performance and hepatic histopathology. Detectable levels of toxins were present in the feed before feeding, and all levels of mycotoxins were above the normal limit. A total of 180 one-day-old male Arbor Acres broiler chickens were allocated randomly to six treatment groups with three replicates per group, including ten chickens per replicate. The experiment lasted for five weeks, and dietary treatments included control diet and diets with four levels of nanosilica as 0.05%, 0.10%, 0.15%, and 0.20% as well as 0.50% bentonite (fixfin® Dry) diet. Bodyweight, body weight gain, average daily feed intake, and feed conversion ratio were measured weekly. At the end of the fifth week, six chickens per treatment were sacrificed to investigate the effects of NS and bentonite on carcass characteristics and hepatic histopathology. The results showed that providing broiler chickens’ diets with an adsorbent agent, such as NS or bentonite, can reduce the side effects of mycotoxins and enhance their growth performance. The best record was achieved with NS at 0.20%, compared with the control group and other dietary treatment groups. Accordingly, 0.20% of NS could be used in broiler chickens’ diets to minimize the harmful effects of mycotoxins.

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