scholarly journals Antifungal Activity of Various Extracts of Seeds of the Plant Malva Parviflora (Linn)

2017 ◽  
Vol 14 (4) ◽  
pp. 1409-1412
Author(s):  
Jadhav Raina ◽  
Parihar Sangeeta
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antifungal Agent ◽  
Antifungal Agents ◽  
Diffusion Method ◽  
Dilution Method ◽  
Significant Activity ◽  
Water As Solvent ◽  
Malva Parviflora ◽  
Agar Well Diffusion Method

ABSTRACT: Development of more effective and less toxic antifungal agents is required for the treatment of various fungal disease.Plants and their extraction preparation have beenused as medicine against infectious diseases.The present study was aimed to study, the antifungal activity of the seeds of various seed abstract of plant Malva Parviflora (Linn). The antifungal activity of seeds extract of plant was determine by using agar well diffusion method,MIC(minimum inhibitoryconcentration) and MFC (minimum fungicidal count)by using micro dilution method . The seeds extract of the plant were examined using Methanol, Ethyl acetate ,Petroleum ether and water as solvent and tested against different fungi pathogens. From the result it can be concluded that the all the seeds extract shows the significant activity against the micro organism, hence these extract may be used as a source of antifungal agent obtained from herbal medicine and may be explore as new and effective antifungal agent. Various solvent extracts of the plant Malva parvifiora (Linn) have been found to possess enough antibacterial activity and may potentially be explored as human antifungal agent.

scholarly journals Kaolin/Gum Arabic Nanocomposite as Antifungal agent against Aspergillus Flavus and Saccharomyces cerevisiae

2019 ◽  
pp. 2316-2320
Author(s):  
Zaccheus Shehu ◽  
Wilson Danbature Lamayi ◽  
Adunbe Maisanda Sabo ◽  
Sani Ibrahim Aliyu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Antifungal Activity ◽  
Aspergillus Flavus ◽  
Antifungal Agents ◽  
Gum Arabic ◽  
Diffusion Method ◽  
Cork Borer ◽  
Petri Dishes ◽  
Ethanol Extracts ◽  
Agar Well Diffusion Method

Kaolin/Gum Arabic nanocomposite was cheaply synthesized from Kaolin and Gum Arabic. The Kaolin/Gum Arabic nanocomposite suspension, Gum Arabic extracts and Kaolin suspension were applied as antifungal agents. The antifungal activity was tested using agar well diffusion method where by wells were made on the petri dishes with cork borer 6mm diameter in size and various concentrations (150 µg/L, 200 µg/L, and 250 µg/L) of Gum Arabic ethanol extracts, Gum Arabic /Kaolin nanocomposite, and Kaolin was propelled into the wells with the help of micropipette and the petri dishes were allowed to stand for 30 minutes to ensure proper diffusion before being incubated at 37oC. The results showed that synthesized Kaolin/Gum Arabic nanocomposite and Gum Arabic possess significant antifungal activity against Aspergillus flavus and Saccharomyces cerevisiae. No antifungal activity detected for Kaolin against Aspergillus flavus and Saccharomyces cerevisiae. From the results obtained it could be concluded that the synthesized Kaolin/Gum Arabic nanocomposite and Gum Arabic possess significant antifungal activity against Aspergillus flavus and Saccharomyces cerevisiae.

scholarly journals SYNTHESIS, ANTIMICROBIAL, ANTITUBERCULAR AND CHEMINFORMATIC STUDIES OF 2-(1-BENZOFURAN-2-YL)-N'-[(3Z)-2-OXO-1, 2-DIHYDRO-3H-INDOL-3-YLIDENE] QUINOLINE-4-CARBOHYDRAZIDE AND ITS DERIVATIVES

2017 ◽  
Vol 9 (5) ◽  
pp. 260 ◽  
Author(s):  
S. Santoshkumar ◽  
N. D. Satyanarayana ◽  
R. Anantacharya ◽  
P. Sameer
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
In Silico ◽  
Antitubercular Activity ◽  
Mean Value ◽  
Diffusion Method ◽  
Pharmacokinetic Parameters ◽  
Moderate Activity ◽  
Significant Activity ◽  
Standard Drug

Objective: Synthesis of novel 2-(1-benzofuran-2-yl)-N'-[(3Z)-2-oxo-1, 2-dihydro-3H-indol-3-ylidene] quinoline-4-carbohydrazide and its derivatives for antimicrobial and antitubercular activity.Methods: Synthesis was carried out using the general method and the structures were confirmed by IR, 1H-NMR, [13]C-NMR and mass spectral analysis. The antibacterial activity was carried by agar well diffusion method, antifungal activity was performed by poison food technique and antitubercular activity was carried out by Microplate Alamar Blue Assay (MABA) method with the help of H37Rv. In silico absorption, distribution, metabolism, excretion, toxicity (ADMET) study of the drug, likeliness was carried out in ACD/lab-2.Results: The results revealed that at 25 mg/ml concentration, compounds 3a and 5a showed good antibacterial activity at 3.5±0.1, 3.8±0.3, 3.6±0.2 respectively against E. coli, K. pneumonia and S. typhimurium, when compared with drug streptomycin with similar concentration. The percentage of inhibition found at 50 µg/ml concentration, compounds 2b and 6a exhibited good antifungal activity at 53±1.15, 57±1.52 against A. flavus and C. neoformans, compared with standard drug fluconazole. The increase in activity was found to be dose dependent. The analogue 2a showed good antitubercular activity at 12.5±0.5 µg/ml, compounds 2b, 3a, 4a-b, 5a-b and 6a-b exhibited significant activity at 25±0.57 µg/ml and compound 3b showed moderate activity at 50±0.57 µg/ml. The mean value of P<0.05 were considered to be statistically significant. The absorption, distribution, metabolism, excretion and toxicity studies of the entitled molecules were analyzed and found to be in acceptable range.Conclusion: The study reveals that compounds containing benzofuran coupled nitrogen heterocycles are essential for activity as they possess excellent drug-like characteristics, suggesting to be potentially best inhibitor of H37Rv strain. The in silico ADME analysis also revealed that all the compounds were in acceptable range to obey the pharmacokinetic parameters.

Enhancing Antibacterial Activity by Combination of Chloramphenicol with Constituents from Dracaena cochinchinensis (Lour.) S.C.Chen

2018 ◽  
Vol 17 (1) ◽  
pp. 74-80
Author(s):  
Ritbey Ruga ◽  
Warinthorn Chavasiri
Keyword(s):  
Antibacterial Activity ◽  
Spectroscopic Analysis ◽  
Inhibitory Concentration ◽  
Colorimetric Assay ◽  
Optical Rotation ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Well Diffusion Method

Background: Seven compounds were isolated from Dracaena cochinchinensis and elucidated their structure by NMR spectroscopic analysis and determined the optical rotation for certain compounds. Methods: These compounds were screened for antibacterial activity by using agar well diffusion and determination of minimum inhibitory concentration (MIC) was conducted by the broth micro-dilution method using resazurin colorimetric assay. Further investigation on combination effect between each compound with chloramphenicol was conducted by agar well diffusion method. Among the seven compounds, compound 4 displayed the highest antibacterial activity against P. acnes and S. aureus with the inhibition zone of 17.3 and 16.7 mm, respectively. The MIC value of 1 and 4 against all tested bacteria was 62.5 µM, whereas those of other compounds were 62.5-250 µM. The combination of 4 and chloramphenicol exhibited the most synergistic effect against P. acnes with the rate in increasing antibacterial activity of 4 in combination as 1,250 folds. This compound also enhanced the antibacterial activity of chloramphenicol at 50 µM with an inhibition zone of 16.3 mm comparing to its activity alone (8.7 mm). Results & Conclusion: The results revealed compound 4 displayed the highest antibacterial activity alone and in combination with chloramphenicol against P. acnes and S. aureus.

Synthesis and Evaluation of Demethoxyviridin Derivatives as Potential Antimicrobials

2005 ◽  
Vol 60 (9-10) ◽  
pp. 686-692
Author(s):  
Ismail Kiran ◽  
Semra Ilhan ◽  
Tamer Akar ◽  
Lacine Tur ◽  
Erdinc Erol
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Geotrichum Candidum ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Antibacterial And Antifungal Activities ◽  
Agar Dilution ◽  
Antibacterial And Antifungal

Abstract The in vitro antibacterial and antifungal activities of demethoxyviridin and some synthetic analogues were evaluated by the agar diffusion method. The minimum inhibitory concentrations (MIC) of the active compounds were also determined by the agar dilution method. Demethoxyviridin (1) showed moderate antibacterial activity against most of the strains tested. 1α-Hydroxydemethoxyviridin (3) showed antibacterial activity and the most potent in vitro antifungal activity with MIC of 20 μg/ml (0.062 mm) against Aspergillus niger, A. fumigatus, A. flavus, A. parasiticus, Fusarium solani, F. graminarum, Geotrichum candidum whereas 5′-methylfuro-(4′,3′,2′-4,5,6)androst-5-ene-3,17-dione (7) exhibited very weak antifungal activity against Candida albicans only.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

scholarly journals Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Séverine Boisard ◽  
Anne-Marie Le Ray ◽  
Anne Landreau ◽  
Marie Kempf ◽  
Viviane Cassisa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Gram Positive ◽  
Weak Activity ◽  
Agar Dilution

During this study, thein vitroantifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains:Candida albicans, C. glabrata, andAspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains includingStaphylococcus aureus. Organic extracts showed a significant antifungal activity againstC. albicansandC. glabrata(MIC80between 16 and 31 µg/mL) but only a weak activity towardsA. fumigatus(MIC80= 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially againstS. aureus(SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC10030–97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.

GROWTH INHIBITORY ACTIVITIES OF THE RHIZOME CRUDE EXTRACT OF Curcuma longa ON THE HUMAN PATHOGENIC FUNGUS Mucor circinelloides

2020 ◽  
Vol 65 (10) ◽  
pp. 82-91
Author(s):  
Phuong Nguyen Anh ◽  
Mai Le Thi Tuyet ◽  
Trung Trieu Anh
Keyword(s):  
Antifungal Activity ◽  
Crude Extract ◽  
Fungal Infections ◽  
Early Stage ◽  
Curcuma Longa ◽  
Pathogenic Fungus ◽  
Mucor Circinelloides ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Dilution Method

Mucormycosis is an uncommon but life-threatening invasive fungal infection, mostly occurs in immunocompromised patients. Lacking the appropriate antifungal drugs is one of the reasons that lead to difficulties in the management of mucormycosis. Curcuma longa has been used traditionally and widely to treat various diseases, including fungal infections. In the search for novel antifungal compounds from natural resources, we evaluated the effect of rhizome crude extract of C. longa on Mucor circinelloides – a causal agent of mucormycosis. The results of screening, using broth dilution method and agar-well diffusion method, showed that the C. longa extract exhibited promising antifungal activity against the fungus M. circinelloides. In liquid medium, C. longa extract decreased the ability of spore germination and the speed of hyphae formation of M. circinelloides decreased by up to approximately 70% and 90%, respectively. Besides, in a solid medium, the crude extract presented similar activity with amphotericin B (400 μg\mL) in decreasing the growth of M. circinelloides by nearly 77%. Moreover, the extract of C. longa also likely to induce the yeast-like type of growth of the dimorphic M. circinelloides in the early stage. These results suggest the plant could be a potential source for further study on biochemical components and the mechanism of its antifungal activity.

scholarly journals Microwave-Hydrothermal Rapid Synthesis of Cellulose/Ag Nanocomposites and Their Antibacterial Activity

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 978 ◽  
Author(s):  
Lian-Hua Fu ◽  
Qing-Long Gao ◽  
Chao Qi ◽  
Ming-Guo Ma ◽  
Jun-Feng Li
Keyword(s):  
Antibacterial Activity ◽  
Antimicrobial Properties ◽  
Silver Ions ◽  
Heating Time ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Human Beings ◽  
Microwave Hydrothermal ◽  
Wide Range

Silver-based antimicrobial nanomaterials are considered as the most promising antibacterial agents owing to their outstanding antimicrobial efficacy and their relatively low toxicity to human beings. In this work, we report on a facile and environment-friendly microwave-hydrothermal method to prepare cellulose/Ag nanocomposites using hemicellulose as the reductant. The influences of the microwave-hydrothermal heating time and temperature, as well as the hemicellulose concentration on the formation of cellulose nanocomposites, were investigated in detail. Experimental results indicated that the hemicellulose was an effective reductant for silver ions, with higher temperature and longer heating time favoring the formation of silver with higher crystallinity and mass content in the nanocomposites. Moreover, the antimicrobial properties of the as-prepared cellulose/Ag nanocomposites were explored using Gram-positive S. aureus ATCC 6538 and Gram-negative E. coli HB 101 by both disc diffusion method and agar dilution method, and the nanocomposites showed excellent antibacterial activity. These results demonstrate that the as-prepared cellulose/Ag nanocomposites, as a kind of antibacterial material, are promising for applications in a wide range of biomedical fields.

scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF SILVER NANOPARTICLES AGAINST NEISSERIA GONORRHOEAE AND CANDIDA ALBICANS

2020 ◽  
Vol 8 (6) ◽  
pp. 179-187
Author(s):  
Titik Taufikurohmah ◽  
Tasha Anandya Tantyani
Keyword(s):  
Antibacterial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Neisseria Gonorrhoeae ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Disk Diffusion Method ◽  
Fungal Inhibition ◽  
Inhibition Response ◽  
Antibacterial And Antifungal

This Research on the antibacterial and antifungal activity of nanosilver against Neisseria gonorrhoeae and Candida albincas fungi has been carried out. The purpose of this study was to determine antibacterial activity of nanosilver against Neisseria gonorrhoeae and antifungal activity against Candida albincas. Synthesis Nanosilver uses bottom up method and characterized using UV-Vis Spectrophotometer. Nanosliver concentrations used were 30, 40, 50, and 60 ppm. Antibacterial and antifungal activity tests using disk diffusion method. Observations obtained in form of the presence or absence of clear zones formed around paper discs indicate the inhibition of nanosilver on microbial growth. The results of testing the antifungal activity of Candida albicans on nanosilver with concentrations of 30, 40, 50 and 60 ppm resulted in clear zones of 9.73 nm, 11.46 nm, 11.93 nm, and 13 nm with fungal inhibition response categories is medium and strong. The results antibacterial activity test of Neisseria gonorrhoeae on nanosilver with concentrations of 30, 40, 50 and 60 ppm did not show any clear zone around the disc, it showed that nanosilver in this study did not have antibacterial activity against Neisseria gonorrhoeae.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

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