The Antioxidant Activity of Ethanol and Methanol Extracts of Sesame meal by Ultrasonic Method in Comparison with the Synthetic Antioxidants in Iranian Mutton Tallow

Background:: Mushrooms are deemed as a special delicacy in many countries. They are considered an important cuisine due to their bioactive ingredients and possible health benefits. Methods: Herein, we measured selected biological properties of methanol extracts of Pleurotus citrinopileatus and Boletus edulis fruiting bodies including; in vitro antimicrobial activity, anti-α- glucosidase activity, antioxidant activity, anti-lipase activity and cytotoxic activity against different cancer cells and normal cells. Results: B. edulis methanol extracts showed high antimicrobial and anti-α-glucosidase activity. In contrast, P. citrinopileatus methanol extracts showed superior antioxidant activity indicated by (1,1- diphenyl-2-picrylhydrazyl) DPPH radical scavenging with half maximal inhibitory concentration of IC50 37.4 µg/ml, anti-lipase activities with IC50 65.2 µg/ml and high cytotoxicity activity against HepG2 and HeLa cell lines with IC50 22.8 and 36.7 µg/ml, respectively. Flow cytometric analysis of the cell cycle was used to show apoptotic effects of methanol extracts against HepG2 and HeLa cells. Conclusion: P. citrinopileatus and B. edulis methanolic extracts appear to contain biologically active compounds that might be used to treat some common human diseases.

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Antioxidant Compounds from Microalgae: A Review

Marine Drugs ◽

10.3390/md19100549 ◽

2021 ◽

Vol 19 (10) ◽

pp. 549

Author(s):

Noémie Coulombier ◽

Thierry Jauffrais ◽

Nicolas Lebouvier

Keyword(s):

Antioxidant Activity ◽

Natural Products ◽

Mode Of Action ◽

Natural Antioxidants ◽

Culture Conditions ◽

Antioxidant Compounds ◽

Oxygen Species ◽

Synthetic Antioxidants ◽

Metabolic Plasticity ◽

The Right

The demand for natural products isolated from microalgae has increased over the last decade and has drawn the attention from the food, cosmetic and nutraceutical industries. Among these natural products, the demand for natural antioxidants as an alternative to synthetic antioxidants has increased. In addition, microalgae combine several advantages for the development of biotechnological applications: high biodiversity, photosynthetic yield, growth, productivity and a metabolic plasticity that can be orientated using culture conditions. Regarding the wide diversity of antioxidant compounds and mode of action combined with the diversity of reactive oxygen species (ROS), this review covers a brief presentation of antioxidant molecules with their role and mode of action, to summarize and evaluate common and recent assays used to assess antioxidant activity of microalgae. The aim is to improve our ability to choose the right assay to assess microalgae antioxidant activity regarding the antioxidant molecules studied.

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DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s1.ff021 ◽

2020 ◽

pp. 107-111

Author(s):

YUNITRIANTI ◽

BERNA ELYA ◽

ARIKADIA NOVIANI

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Total Flavonoid ◽

Dpph Assay ◽

Frap Assay ◽

Methanol Extracts ◽

Uv Visible ◽

Antioxidant Effects

Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research hasbeen conducted to explore the antioxidant activity of the plant.Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay usinga UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentrationof 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetateand methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, andmethanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had thegreatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mgGAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.

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Natural Antioxidants from Seeds and Their Application in Meat Products

Antioxidants ◽

10.3390/antiox9090815 ◽

2020 ◽

Vol 9 (9) ◽

pp. 815 ◽

Cited By ~ 1

Author(s):

Paulo E. S. Munekata ◽

Beatriz Gullón ◽

Mirian Pateiro ◽

Igor Tomasevic ◽

Ruben Domínguez ◽

...

Keyword(s):

Antioxidant Activity ◽

Food Industry ◽

Meat Products ◽

Natural Antioxidants ◽

Synthetic Antioxidants ◽

Oxidative Reactions ◽

Health Related ◽

Processing And Storage ◽

And Storage

The use of synthetic antioxidants in the food industry has raised important questions about the effects of prolonged consumption on human health. On top of that, the consumption of meat products has been changing due to the awareness generated by health-related organizations. In this sense, exploring strategies to develop and produce healthier meat products has become a paramount concern. Several studies explored the composition of several seeds to characterize and explore the compounds with antioxidant activity, which are mainly composed of polyphenols. The use of antioxidant extracts in meat products has shown important results to delay the oxidative reactions in meat products derived from the processing and storage of meat products. Moreover, these extracts can also replace synthetic antioxidants and preserve the quality of meat products. Therefore, the aims of this review are first, to present the sources and compounds with antioxidant activity in seeds, and second, to discuss their protective effect against oxidative reactions in meat products.

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ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i8.25922 ◽

2018 ◽

Vol 10 (8) ◽

pp. 36 ◽

Cited By ~ 4

Author(s):

Muhammad Dawood Shah ◽

Mohammad Iqbal

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Ethyl Acetate ◽

Palmitic Acid ◽

Methanol Extract ◽

Radical Scavenging ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Hexadecanoic Acid ◽

Methanol Extracts

Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained from Commelina nudiflora were investigated for the free radical scavenging effects and phytochemical analysis.Methods: The antioxidative effect of the essential oil, methanol extracts and methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and ﬂavonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively. The phytochemical analyses of the essential oil, methanol extracts and methanol fractions were performed by gas chromatography and mass spectrometry (GCMS). Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetate and chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoids and tannins etc. The identified bioactive constituents of essential oil, methanol extract and methanol fractions of C. nudiflora were indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl, phenol, benzyl alcohol, eugenol, phenol, 2, 4-bis (1,1-dimethylethyl), hexadecanoic acid, ethyl ester (palmitic acid ester), n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid) and phytol. All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractions of C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.

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Deposition Efficacy of Natural and Synthetic Antioxidants on Fabrics

Applied Sciences ◽

10.3390/app10186213 ◽

2020 ◽

Vol 10 (18) ◽

pp. 6213

Author(s):

Paola Giannì ◽

Heiko Lange ◽

Giulia Bianchetti ◽

Conny Joos ◽

David W. Brogden ◽

...

Keyword(s):

Antioxidant Activity ◽

Cotton Fabric ◽

Analytical Method ◽

Pine Bark ◽

Phenolic Antioxidants ◽

Synthetic Antioxidants ◽

Solid Matrices ◽

Natural And Synthetic

Pine bark extracts were tested as valid natural alternatives to common phenolic antioxidants to be deposited onto solid matrices. An analytical method for determining both deposition-efficacy and maintenance of antioxidant activity on fabrics surfaces was developed and successfully applied to phenolic antioxidants and pine tannins onto cotton fabric samples. Accumulated data suggest that common phenolic antioxidants and tannin contribute to an elevated antioxidant activity on fabric. A known source of odour is autoxidation of residual sebum on fabric, leading to the generation of malodourous compounds. Therefore, antioxidants hold the promise for mitigating malodour on fabrics, a top unmet consumer need around the globe.

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The Hypoglycemic and Antioxidant Activity of Cress Seed and Cinnamon on Streptozotocin Induced Diabetes in Male Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/5614564 ◽

2016 ◽

Vol 2016 ◽

pp. 1-15 ◽

Cited By ~ 6

Author(s):

Safaa Qusti ◽

Haddad A. El Rabey ◽

Sarah A. Balashram

Keyword(s):

Antioxidant Activity ◽

Blood Sugar ◽

Positive Control ◽

Diabetic Group ◽

Fasting Blood Sugar ◽

Histopathological Changes ◽

Garden Cress ◽

Methanol Extracts ◽

Cress Seed ◽

Streptozotocin Induced Diabetes

The present study aimed to estimate the stimulation of pancreas of rats with streptozotocin induced diabetes using 20% (w/w) garden cress seed (Lepidium sativum) and cinnamon methanol extracts. The positive control diabetic group showed a significant increase in fasting blood sugar, lipid peroxide, interleukin-6, carboxymethyl lysine, serum uric acid, urea, creatinine, immunoglobulins, and urine albumin and a significant decrease in antioxidant enzymes, sodium ions, potassium ions, and urine creatinine. Severe histopathological changes in the kidney and pancreas tissues in hyperglycemic rats were also shown in the positive control diabetic group. Meanwhile, the groups that were treated with 20% garden cress seed and cinnamon methanol extracts showed a significant decrease in fasting blood sugar and all elevated abovementioned biochemical parameters and an increase in the lowered ones restoring them nearly to the normal levels of G1. Kidney and pancreas tissues were also ameliorated and restored nearly to the normal status. Both garden cress seed and cinnamon methanol extracts succeeded in controlling hyperglycemia in rats with streptozotocin induced diabetes and ameliorated the biochemical and histopathological changes because of their antioxidant activity acquired by their possession of phenolic phytochemicals.

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Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela

Ciência Rural ◽

10.1590/0103-8478cr20170772 ◽

2018 ◽

Vol 48 (6) ◽

Cited By ~ 2

Author(s):

Caroline Pagnossim Boeira ◽

Natiéli Piovesan ◽

Marcela Bromberger Soquetta ◽

Déborah Cristina Barcelos Flores ◽

Bruna Nichelle Lucas ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Bioactive Compounds ◽

Extraction Method ◽

Ultrasonic Method ◽

Ultrasound Assisted Extraction ◽

Ultrasound Method ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Conventional Extraction

ABSTRACT: The aim of this study was to evaluate the effect of extraction conditions on bioactive compounds, as well as on antioxidant activity, and the antimicrobial activity of the extracts with the highest antioxidant characteristics. The extracts were obtained by conventional method and ultrasound-assisted extraction at various temperatures (20°C, 40°C and 60°C). Total phenolics, total flavonoids, antimicrobial activity and antioxidant activity were quantified by the methods of DPPH, FRAP, and ORAC, respectively. The conventional extraction method and ultrasound method influenced the phenolic content at all the tested temperatures. Flavonoids were not influenced by extraction methods. The antioxidant activity (DPPH) was highest in the ultrasonic method at temperatures of 40°C and 60°C; however, in the case of the FRAP method the best results were for the conventional extraction method. The conventional and ultrasonic methods did not influence the IC50 at temperatures of 20°C and 40°C, but using ORAC the antioxidant activity was influenced by the methods at all temperatures. The extract obtained at 60°C by the ultrasound method had high antimicrobial action in relation to the strains of Salmonella sp., Escherichia coli, and Staphylococcus aureus. Extraction ultrasonic-assisted can be considered adequate to obtain extracts of marcela, which are rich in bioactive compounds with high antioxidant activity.

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Antioxidant Activity of Methanol Extracts Obtained fromPlantagoSpecies

Journal of Agricultural and Food Chemistry ◽

10.1021/jf048076s ◽

2005 ◽

Vol 53 (6) ◽

pp. 1927-1933 ◽

Cited By ~ 104

Author(s):

Marina Gálvez ◽

Carmen Martín-Cordero ◽

Peter J. Houghton ◽

María Jesús Ayuso

Keyword(s):

Antioxidant Activity ◽

Methanol Extracts

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