scholarly journals Liver metabolic activities of Pasundan cattle induced by irradiated chitosan

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Andi Mushawwir ◽  
JOHAR ARIFIN ◽  
DARMAWAN DARWIS ◽  
TITA PUSPITASARI ◽  
DEWI SEKAR PENGERTENI ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Control Group ◽  
Cattle Breeding ◽  
Blood Samples ◽  
Treatment Groups ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer ◽  
Metabolic Activities ◽  
Development Center ◽  
Growth And Reproduction

Abstract. Mushawwir A, Arifin J, Darwis D, Puspitasari T, Pengerteni DS, Nuryanthi N, Perman R. 2020. Liver metabolic activities of Pasundan cattle induced by irradiated chitosan. Biodiversitas 21: 5571-5578. A total of one hundred and twenty-five, 2-3 year old male Pasundan cattle were used as livestock samples during the three months of this research. They were selected from the local cattle breeding and development center in Ciamis. The animal samples were randomly allocated to 5 treatment groups. One group served as the control, or without irradiated chitosan, while the others were used as treatment in varying levels. Each treatment group involved five replicates with 25 Pasundan bulls per treatment i.e five Pasundan bulls per replication. Each group was provided with the following rations: C0 = Control group, without IC (0 ppm IC); C1 = 350 ppm Irradiated Chitosan (IC); C2 = 400 ppm IC; C3 = 450 ppm IC; and C4 = 500 ppm IC. Irradiated chitosan was obtained through the following steps: extraction, deacetylation, and irradiation of chitin using gamma rays. Five mL of blood samples were collected from each bull at the beginning of each month of this experiment, which totaled three months. The blood samples were sucked from the tail/coccygeal vein using a sterilized syringe and vacuum tube containing K3EDTA. The plasma was used to determine the concentration of parameters related to liver metabolism through an automatic biochemical analyzer Kenza 240TX model from Biolabo, using a commercial kit. Each procedure was followed based on the Biolabo kit (Franch) and Randox kit (UK). This study showed that IC reduces the activity of glycogenolysis and glycolysis, but is accompanied by improvements in the biochemical conditions of liver cells. This is a favorable condition for the metabolism of Pasundan bulls in order to enhance their growth and reproduction.

Renal function in the laboratory rat: a student exercise.

1995 ◽  
Vol 268 (6) ◽  
pp. S49 ◽  
Author(s):  
R L Walker ◽  
M E Olson
Keyword(s):  
Renal Function ◽  
Fluid Intake ◽  
Tap Water ◽  
Extracellular Fluid ◽  
Control Group ◽  
Blood Samples ◽  
Treatment Groups ◽  
Laboratory Rats ◽  
Group A

Because of the increased concern over use of human body fluids in physiology teaching laboratories, we developed an exercise in renal function that utilizes laboratory rats. The purpose is to demonstrate the role of the kidneys in the homeostatic control of extracellular fluid volume, plasma ionic concentrations, and osmolarity. Three treatment groups are utilized: a volume-expanded (access to 1 g/100 ml sucrose) group, a volume-expanded and salt-loaded (access to 0.9 g/100 ml NaCl) group, and a volume-depleted (water-deprived) group. A normovolemic control group (access to tap water) is also included. Rats are housed individually in metabolic cages that allow accurate measurement of fluid intake and urine output. Blood samples are removed via cardiac puncture. The animals recover from this procedure and can be reutilized within 2-3 wk. When class data are pooled, clear trends are seen that demonstrate the volume-, osmo-, and ionoregulatory abilities of the kidneys.

scholarly journals Comparative Efficacies of Amoxicillin, Clindamycin, and Moxifloxacin in Prevention of Bacteremia following Dental Extractions

2006 ◽  
Vol 50 (9) ◽  
pp. 2996-3002 ◽  
Author(s):  
P. Diz Dios ◽  
I. Tomás Carmona ◽  
J. Limeres Posse ◽  
J. Medina Henríquez ◽  
J. Fernández Feijoo ◽  
...  
Keyword(s):  
Prophylactic Treatment ◽  
Venous Blood ◽  
Control Group ◽  
Anesthesia Induction ◽  
Beta Lactams ◽  
Blood Samples ◽  
Treatment Groups ◽  
Microbiological Techniques ◽  
Group A ◽  
Aerobic And Anaerobic

ABSTRACT We evaluated the efficacies of oral prophylactic treatment with amoxicillin (AMX), clindamycin (CLI), and moxifloxacin (MXF) in the prevention of bacteremia following dental extractions (BDE). Two hundred twenty-one adults who required dental extractions under general anesthesia were randomly assigned to a control group, an AMX group, a CLI group, and an MXF group (the individuals in the drug treatment groups received 2 g, 600 mg, and 400 mg, respectively, 1 to 2 h before anesthesia induction). Venous blood samples were collected from each patient at the baseline and 30 s, 15 min, and 1 h after the dental extractions. The samples were inoculated into BACTEC Plus aerobic and anaerobic blood culture bottles and were processed in a BACTEC 9240 instrument. Subculture and the further identification of the isolated bacteria were performed by conventional microbiological techniques. The prevalences of BDE in the control group, AMX group, CLI group, and MXF group were 96, 46, 85, and 57%, respectively, at 30 s; 64, 11, 70, and 24%, respectively, at 15 min; and 20, 4, 22, and 7%, respectively, at 1 h. Streptococcus spp. were the most frequently identified bacteria in all groups (44 to 68%), with the lowest percentage being detected in the AMX group (44%). AMX and MXF prophylaxis showed high efficacies in reducing the prevalence and duration of BDE, but CLI prophylaxis was noneffective. As a consequence, MXF prophylaxis is a promising antibiotic alternative for the prevention of BDE when beta-lactams are not indicated.

scholarly journals Evaluation of Colocasia esculenta Schott in anti-cancerous properties with proximity extension assays

2021 ◽  
Author(s):  
Liang Wu ◽  
Yuxuan Wang ◽  
Xiaoyan Wang ◽  
Jun Liao ◽  
Hao Dong ◽  
...  
Keyword(s):  
Tap Water ◽  
Colocasia Esculenta ◽  
Blood Samples ◽  
Protein Levels ◽  
Local Cultivar ◽  
Dietary Consumption ◽  
Before And After ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer ◽  
Prostate Cancers

Background: Colocasia esculenta Schott (called as Xiangshayu in Chinese) is an excellent local cultivar of the genus polymorpha in Jiangsu Province, China. Objective: In the present study, we have performed a comparative study before and after dietary consumption with Colocasia esculenta Schott to evaluate its anti-cancerous properties. Design: Forty-two healthy volunteers were recruited, and dietary consumption with 200 g of tap water cooked Colocasia esculenta Schott daily was conducted for 1 month. Plasma samples from the subjects before and after dietary consumption with Colocasia esculenta Schott were analyzed with proximity extension assays for the alteration of 92 proteins in relation with cancers, while blood samples were examined for physiological parameters with an automatic biochemical analyzer. Bioinformatic analyses were conducted using MalaCards and GEPIA. Results: After taking dietary consumption with Colocasia esculenta Schott, circulating CYR61, ANXA1, and VIM protein levels in the subjects was found to be most significantly downregulated, while for ITGB5, EPHA2, and CEACAM1, it was upregulated. Alternation of these proteins was predicted to be associated with the development of tumors such as pancreatic adenocarcinoma and breast and prostate cancers. Conclusion: The present study provides evidence that Colocasia esculenta Schott, as a healthy food, has anti-cancerous properties. Further investigation of phytochemistry in Colocasia esculenta Schott has been taken into our consideration.

scholarly journals Comparison of four methods of inducing pseudopregnancy in rabbits

2022 ◽  
Vol 78 (01) ◽  
pp. 6608-2022
Author(s):  
SYAFRUDDIN SYAFRUDDIN ◽  
SRI WAHYUNI ◽  
GHOLIB GHOLIB ◽  
TONGKU NIZWAN SIREGAR
Keyword(s):  
Positive Control ◽  
The Other ◽  
Control Group ◽  
Corpora Lutea ◽  
Histological Observation ◽  
Blood Samples ◽  
Treatment Groups ◽  
Examination Time ◽  
Significant Difference ◽  
The Difference

This study was conducted to compare several methods for inducing pseudopregnancy in local rabbits. The study used 25 clinically healthy rabbits of local breeds, aged 1-1.5 years, with a bodyweight of 1.8-2.2 kg. The rabbits were divided into five treatment groups (n = 5): R1, R2, R3, R4, and R5. Each group was given a different treatment. Group R1 was injected intramuscularly with 100 IU PMSG and mated with males three days later, which was followed by an intravenous injection of 75 IU hCG. Pseudopregnancy in group R2 was induced through artificial copulation by inserting a 1 cm long cotton bud into vaginal lumen at 5 a.m. Pseudopregnancy in group R3 was induced using 50 IU hCG intramuscularly. Group R4 was injected intravenously with 5 g GnRH and then intramuscularly with 100 IU PMSG and three days later with 75 IU hCG. Blood samples were collected on days 2, 4, 6, and 8 for estrogen and progesterone measurement, whereas an ovarian histological observation of all groups was performed on day 8. The results show that the highest concentration of estrogen was obtained in group R2, which was significantly different from the other groups (P <0.05), but the difference in examination time was not statistically significant (P > 0.05). Furthermore, the concentration of progesterone in all groups fluctuated at various times of the day. Progesterone concentrations were higher in group R5, in which pseudopregnancy was induced with PMSG and hCG. Group R5 showed a significant difference (P <0.05) with R2, R3, and R4, but the difference with the positive control group (R1) was not significant (P > 0.05). The numbers of corpora lutea in groups R1, R2, R3, R4, and R5 were, respectively, 6.87 ± 1.58, 6.22 ± 2.71, 6.74 ± 1.94, 5.98 ± 2.04, and 8.8 ± 1.90, and the largest diameter was obtained in R5 (1.65 ± 0.37 mm). In conclusion, the best method of inducing pseudopregnancy in local rabbits is the administration of PMSG and hCG.

scholarly journals Oral single dose of allopurinol in thoroughbred foals born from mares with placentitis

2016 ◽  
Vol 46 (6) ◽  
pp. 1119-1125
Author(s):  
Luciana Oliveira de Araujo ◽  
Carlos Eduardo Wayne Nogueira ◽  
Fernanda Maria Pazinato ◽  
Friedrich Frey Junior ◽  
Silvano Costa Paixão ◽  
...  
Keyword(s):  
Single Dose ◽  
Adverse Effects ◽  
Serum Calcium ◽  
Group Treatment ◽  
Control Group ◽  
Blood Samples ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Wbc Count ◽  
Thoroughbred Foals

ABSTRACT: The aim of this study was to evaluate the effects of Allopurinol in foals born from mares with placentitis. Twenty foals were assigned into two groups: Healthy foals (n=10), born from healthy mares and Placentitis foals (n=10), born from mares with placentitis. Five foals from each group were randomly assigned to a treatment or control group. Treatment groups received Allopurinol (40mg kg-1 orally six hours after birth). Blood samples were collected for estimation of hematological variables and serum concentration of calcium, chloride, creatinine, phosphorus, glucose, lactate and magnesium. Placentitis foals presented leukopenia and neutropenia when compared with Healthy foals, at birth. The white blood cell (WBC) count was lower in the Placentitis foals untreated at 12 hours. No adverse effects related to the use of Allopurinol were detected. Treated Placentitis foals showed higher serum calcium and glucose levels within 12 hours than untreated Placentitis foals. Administration of Allopurinol PO in foals born from mares with placentitis did not result in adverse effects and can help in stabilizing serum calcium and glucose levels.

scholarly journals Antioxidant Effect of Virgin Coconut Oil on Urea and Creatinine Levels on Maximum Physical Activity

2019 ◽  
Vol 7 (22) ◽  
pp. 3781-3785
Author(s):  
Fajar Apollo Sinaga ◽  
Urip Harahap ◽  
Jansen Silalahi
Keyword(s):  
Physical Activity ◽  
Tissue Injury ◽  
Coconut Oil ◽  
Maximal Activity ◽  
Male Rats ◽  
Control Group ◽  
Healthy Male ◽  
Virgin Coconut Oil ◽  
Blood Samples ◽  
Treatment Groups

BACKGROUND: Maximal physical activity can produce an imbalance between reactive oxygen species (ROS) and antioxidants which are possibly related to fatigue and tissue injury. One of the natural sources that contain antioxidants is virgin coconut oil (VCO). AIM: This study aimed to determine the protective effects antioxidant of virgin coconut oil (VCO) treatment on urea and creatine level on maximum physical activity METHODS: This study used 24 healthy male rats. The rats were divided into four groups, randomly consisted of six rats in each group. The control group (P0) was given 2 mL water, the treatment groups (VCO-1, VCO-2, and VCO-4) were given VCO 1 mL/200 gBW, 2 mL/200 gBW and 4 ml/200 gBW, respectively, per day using gavage spuit. After 28 days, the rats were forced to perform maximal activity by putting the rats in water with no exit. Blood samples were collected immediately after the maximum physical activity. The urea, creatinine, malondialdehyde and glutation peroxidase level was then measured. RESULTS: This study used 24 healthy male rats. The rats were divided into four groups randomly consisted of six rats in each group. The control group (P0) was given 2 mL water, the treatment groups (VCO-1, VCO-2, and VCO-4) were given VCO 1 mL/200 gBW, 2 mL/200 gBW and 4 ml/200 gBW, respectively, per day using gavage spuit. After 28 days, the rats were forced to perform the maximal activity by putting the rats in water with no exit. Blood samples were collected immediately after the maximum physical activity. The urea, creatinine, malondialdehyde and glutathione peroxidase level was then measured. CONCLUSION: The results of this study indicate that virgin coconut oil is effective in the prevention of oxidative stress following maximum physical activity.

scholarly journals Determination of the activity of selected antioxidant enzymes during bovine laminitis, induced by oligofructose overload

2020 ◽  
Vol 76 (05) ◽  
pp. 6398-2020
Author(s):  
MUHAMMAD ABID HAYAT ◽  
JIAFENG DING ◽  
YUEPENG LI ◽  
XIANHAO ZHANG ◽  
JIANTAO ZHANG ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Reduced Glutathione ◽  
Tap Water ◽  
Treated Group ◽  
Control Group ◽  
Activity Levels ◽  
Blood Samples ◽  
Treatment Groups ◽  
Dairy Heifers

Alimentary oligofructose (OF) overload induces acute laminitis in dairy heifers. We examined the correlation between acute bovine laminitis and antioxidant levels by measuring the activities of malondialdehyde (MDA), catalase (CAT), reduced glutathione (GSH), and superoxide dismutase (SOD) in dairy heifers with acute bovine laminitis. A total of 12 clinically healthy non-pregnant Chinese Holstein heifers, aged between 18-26 months (20.67 ± 3.01 mo), weighing 335-403 kg (379.71 ± 19.87 kg), and with BCS ranging from 2.7 to 3.3 were selected and divided into two groups of six animals: an OF-treated group and a control group. The OF-treated heifers (n = 6) received 17 g/kg BW of oligofructose dissolved in 2 L/100 kg BW of tap water, whereas the control heifers (n = 6) received 2 L/100 kg BW of tap water. Blood samples of OF-treated and the control heifers were collected at –72 h before, as well as 0, 6, 12, 18, 24, 36, 48, 60 and 72 h after OF overload. Malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), and catalase (CAT) activity levels were measured by authenticated standard methods using blood samples. The mean concentration of superoxide dismutase (SOD) was significantly lower (p < 0.01) in the OF-treated heifers from 24 h to 72 h and significantly lower (p < 0.05) at 18 h compared to the control group. Malondialdehyde (MDA) level was significantly higher (p < 0.01) at 24 h to 72 h and significant increased (p < 0.05) at 12 h to 18 h in OF-treated heifers compared with healthy heifers. There were no significant differences in GSH and CAT activities between the control and treatment groups. Our study showed that inadequate levels of antioxidants (enzymatic and non-enzymatic) may be linked to oxidative stress in sick heifers. OF-treated heifers had decreased SOD and increased MDA in response to acute laminitis. Therefore, SOD, GSH, CAT, and MDA activities may play a dynamic role in the etiopathogenesis of acute laminitis in dairy heifers. However, further detailed research is required to describe these responses and to develop strategies to control acute laminitis.

scholarly journals Hematological and antioxidants responses of dairy cow fed with a combination of feed and duckweed (Lemna minor) as a mixture for improving milk biosynthesis

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
Ujang Hidayat Tanuwiria ◽  
Andi Mushawwir
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Physiological Condition ◽  
Lemna Minor ◽  
Hematological Parameters ◽  
Blood Samples ◽  
Hematology Analyzer ◽  
Biochemical Analyzer ◽  
Automatic Biochemical Analyzer ◽  
Commercial Kit

Abstract. Tanuwiria UH, Mushawwir A. 2020. Hematological and antioxidants responses of dairy cows fed with a combination of feed and duckweed (Lemna minor) as a mixture for improving performance. Biodiversitas 21: 4741-4746. A total of twenty-five, 5-7th-lactation-old or 7-8thyears-old dairy cows were used in the current study to study the effect of feed duckweed (Lemna minor) on the hematological status and parameters related antioxidant in the dairy cow. Each group of treatment involved 5 replicates with a dairy cow each (5 dairy cows per group). All of the group was provided ration following F0 = Uncultivated grass (UG) 60% and supplemented concentrated (C) 40%; F1 = UG 50%+fresh  duckweed 10%+C 40%; F2 = UG 60%+fresh  duckweed 3%+C 37%; F3 = UG 50%+fresh  duckweed 10%+dried  duckweed  3%+C 37%; F4 = UG 45%+fresh  duckweed 15%+dried  duckweed  2%+C 38%. Blood samples were collected from each dairy cow monthly beginning at the first month of this experiment, from the tail vein (vena coccygeal), using a sterilized syringe and vacuum tube containing K3EDTA. Hematological parameters were analyzed by a hematology analyzer. The blood samples collected were also centrifuged to separate the plasma. The plasma was used to determine of concentration of parameters related to antioxidant by an automatic biochemical analyzer, using a commercial kit. All procedure of the analysis was following based on the Biolabo kit (Franch) and Randox kit (UK). Based on in this current study showed that increased hematological condition and antioxidants profile in dairy cow supplemented duckweed . Supplemented duckweed  can be improved physiological condition (hematologic and antioxidants) in the dairy cow.

scholarly journals The effect of aqueous Aloe vera gel extract on serum mineral compositions of Red Sokoto Bucks (Maradi)

2015 ◽  
Vol 42 (2) ◽  
pp. 209-217
Author(s):  
S. A. Ameen ◽  
I. A. Odetokun ◽  
L. O. Raji ◽  
K. T. Biobaku ◽  
G. J. Akorede ◽  
...  
Keyword(s):  
Calcium Chloride ◽  
Total Protein ◽  
Aloe Vera ◽  
Control Group ◽  
Blood Samples ◽  
Treatment Groups ◽  
Aloe Vera Gel ◽  
Significant Difference ◽  
Mineral Compositions ◽  
And Control

This study was conducted to determine the effect of Aloe vera gel extract on the serum mineral compositions of Red Sokoto bucks. Blood samples were collected from 30 bucks before the commencement of administration of Aloe vera gel extract for serum minerals and these served as control group. The bucks were now divided into three groups A, B and C containing ten goats per group. The Aloe vera extract was administered orally at 1.0 ml/kg body weight daily at 3.0%, 4.0% and 5.0% to the group A, B, and C respectively for 14 days period. Five milliliters (5ml) of blood samples were collected from the jugulars vein of Red Sokoto bucks in the respective animals in the groups at 7 and 14 days, deposited into anticoagulant free plastic tubes and allowed to clot at room temperature within 3 hours of collection.Sera samples were analysed for potassium, sodium, calcium, Chloride, total protein, and albumin. Data were analysed using one way analysis of variance (ANOVA). The results showed that there were significant (P<0.05) differences in the values of potassium, calcium, chloride, total protein and albunin in the pretreated control group and post treatment groups while there were no significant difference in sodium level (P>0.05) in the bucks that were administered with Aloe vera gel extract and control group. This indicated that oral Aloe vera gel administration in Red Sokoto bucks relatively caused some changes in mineral composition which may affect some physiological functions of visceral organs in these animals.

scholarly journals Irradiation of intraerythrocytic Plasmodium berghei with a fractionated dose of gamma rays does not effectively reduce the infectivity in mice Mus musculus

2019 ◽  
Vol 4 (1) ◽  
pp. 18-26
Author(s):  
Mukh Syaifudin ◽  
Siti Nurhayati ◽  
Darlina Darlina ◽  
Yanti Lusiyanti ◽  
Teja Kisnanto
Keyword(s):  
Single Dose ◽  
Gamma Radiation ◽  
Gamma Rays ◽  
Malaria Vaccine ◽  
Control Group ◽  
Γ Radiation ◽  
Treatment Groups ◽  
Blood Smears ◽  
Parasitemia Level ◽  
Γ Rays

Malaria infection kills more than one million human every year, mainly under-5-year-old children, including in South East Asian nations. Gamma radiation given at a single dose is commonly used to create the attenuated Plasmodium parasites to get vaccine materials. However, there is no study on the infectivity of parasites after fractionated γ-radiation. This study aimed to assess the infectivity of parasites after irradiated with fractionated γ-rays in mice. A number of Plasmodium bergheithat was irradiated in two fractions of 100 and 50 Gy, 100 and 75 Gy; and 100 and 100 Gy within 5 minutes of interval time was injected intraperitoneally into 12 mice. Mice injected with unirradiated parasites (0 Gy) served as a control group. The parasitemia level of intraerythrocytic parasites in each group was observed at days post injection up to 20 days by making Giemsa stained thin blood smears and observed under the microscope. Results showed that fractionation radiation did not effectively attenuate the parasites where they still grew in blood of mice, except for 100+75 Gy. There are no significant differences among the treatment groups (p0.05). This is different from irradiation at the single dose that resulted in almost completely attenuated parasites mainly the dose of 150 Gy. This implicating that irradiation of gamma rays at a single dose is a better way to mitigate parasites than fractionation dose as the infectivity of irradiated parasites were lower compared to that of fractionated dosage. Keywords: Malaria vaccine, Gamma radiation, Fractionation, Parasitemia

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