scholarly journals Analgesic effectiveness comparison between red betel leaf extract (Piper crocatum) and therapy dosage of aspirin in mice

2017 ◽  
Vol 11 (1) ◽  
pp. 1-6
Author(s):  
DIAN AJENG ATIKANINGRUM ◽  
ENDANG EDININGSIH ◽  
CR. SITI UTARI
Keyword(s):  
Treatment Group ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Betel Leaf ◽  
Significant Difference

Atikaningrum DA, Ediningsih E, Utari CRS. 2013. Analgesic effectiveness comparison between red betel leaf extract (Piper crocatum) and therapy dosage of aspirin in mice. Biofarmasi 11: 1-6. The aim of this research was to find out an analgesic effectiveness of red betel leaf extract (Piper crocatum) compared to the therapy dosage of aspirin in mice. This research used a completely randomize experimental design. The subject in this research were 30 male mice of Swiss which in age of 2-3 months with 20-30 grams weight. The subjects were divided into five treatment groups: (i) negative control group (aquadest), (ii) positive control group (aspirin), (iii) first treatment group (3.64 mg red betel leaf extract), (iv) second treatment group (7.28 mg red betel leaf extract), and (v) third treatment group (14.56 mg red betel leaf extract). Analgesic effect was determined by counting the mice jump on 42oC in hotplate during 5 minutes, 2 hours after treatment. The obtained data were tested statistically by Anova and Pos-Hoc processed by Lead Significance Difference. The Anova test showed that there were significant differences among five treatment groups, while the LSD test showed that there were significant difference between negative control group and treatment groups, and the second and third treatment groups showed no significant difference with positive control group. The red betel leaf extract had an analgesic effect when it was given orally in mice. The treatment groups that had the same efficacy with the aspirin treatment group were the second (7.28 mg red betel leaf extract) and the third groups (14.56 mg red betle leaf extract).

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Topical application of snail mucin gel enhances the number of osteoblasts in periodontitis rat model

2019 ◽  
Vol 52 (2) ◽  
pp. 61
Author(s):  
H. Hendrawati ◽  
Hanindya Noor Agustha ◽  
Rezmelia Sari
Keyword(s):  
Treatment Group ◽  
Negative Control Group ◽  
Repair Process ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Important Indicator ◽  
Rat Models ◽  
Bone Damage ◽  
Significant Difference

Background: Repair of bone damage represents a fundamental issue in the treatment of periodontitis. The important indicator employed to monitor the bone damage repair process is the number of osteoblast cells. Achatina Fulica snail mucin (SM) contains glycosaminoglycans which have the potential to increase their number. However, the use of SM in dentistry remains limited. Purpose: To determine and prove the effect of SM gel in increasing the number of osteoblasts in rat models suffering from periodontitis. Methods: This study used 36 rat models divided into three groups, namely; a treatment group (T: 20% snail mucin gel, n = 12), a positive-control group (P: hyaluronic acid gel, n = 12) and a negative-control group (N: CMC-Na gel, n = 12). 0.2 ml of all material was applied to a pocket by means of a tuberculin syringe once a day for 14 days. Histologic observations using Haematoxylin-Eosin staining were carried out on days 3, 5, 7 and 14. Data was analyzed by two-way ANOVA followed by a post-hoc LSD. Results: A significant difference existed between the number of osteoblasts in the test groups. The highest number of osteoblasts observed was consistently that in the treatment group. Conclusion: The application of 20% snail mucin gel was effective in enhancing the number of osteoblasts in rats suffering from periodontitis.

scholarly journals Extract effect of Tintir (Jathropa Multifida Linn) to Total PMN and Selt Mast in Wound Healing Model Rats with Acute Injury

2019 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Wahyuningsih Wahyuningsih
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Significant Difference ◽  
Pmn Leukocytes ◽  
Stem Extract

Wound is considered as a serious health problem, because it can interfere with quality of life due to pain, weakness, and decreased mobility and quality of life. This study aims to determine the effect ofj jathropha multifida, linn to the number of PMN and selt mast in the process of wound healing in rats with acute injury models. This study is an experimental research laboratory using the design of post test control group design Wistar rats were used as research subjects. Based on Tukey HSD test results, the average number of PMN leukocytes third day the negative control group had no significant difference in the positive control group (0.037) and treatment group (0004). On the seventh day the number of PMN leukocytes negative control group had no significant difference in the positive control group (0.069) and had a significant difference to the treatment group (0.00). Meanwhile, on the fourteenth day the number of PMN leukocytes negative control group had no significant difference in the positive control group (0.000) and had a significant difference to the treatment group (0.00). Based on the results of data analysis Annova one direction on the third day obtained a significant level of 0:50 means there is no significant difference in the number of mast cells in each group on the seventh day. While on day three significant values ​​of 0:08 was also no significant difference, as well as on the fourteenth day found no significant difference between the group with a value of 0.68. Conclusions there was a decrease in the number of Leukocyte PMN in rat wounds given a 10% stem extract ointment, and there was a decrease in the number of mast cells in the wounds of rodents given a 10% stem extract ointment. Suggestions need to be investigated regarding the use of distance stem extract in various concentrations to obtain effective results for wound healing and the need to examine other factors related to wound healing.

scholarly journals Haematological Indices in Trypanosoma Brucei Brucei (Federe Isolate) Infected Nigerian Donkeys (Equus Asinus) Treated with Homidium and Isometamidium Chloride

2017 ◽  
Vol 40 (1) ◽  
pp. 73-82 ◽  
Author(s):  
Queen Nneka Oparah ◽  
Anthony Bedu kojo Sackey ◽  
Idris Alao Lawal ◽  
Usman Shehu Abdullahi
Keyword(s):  
Treatment Group ◽  
Post Infection ◽  
Haemoglobin Concentration ◽  
Chloride Ion ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Equus Asinus

Abstract The efficacy of intramuscular administration of Homidium chloride (Novidium®) and Isometamidium chloride (Sécuridium®) in Nigerian donkeys (Equus asinus) experimentally infected with T. b. brucei (Federe isolate) was investigated. Changes in haematological and serum biochemical indices were evaluated using clinical haematology and biochemistry methods. Red blood cell (RBC) count for the negative control group was significantly higher than for the positive control, Novidium® and Sécuridium®-treatment groups. Haemoglobin (Hb) concentration significantly reduced in the infected untreated group compared with other groups. Packed cell volume (PCV) was significantly different between negative and positive controls, and also between the infected untreated and treatment groups. There was significant reduction in platelet counts post-infection and post-treatment. Mean corpuscular volume (MCV) increased significantly in the treatment groups while mean corpuscular haemoglobin concentration (MCHC) significantly reduced only in the Sécuridium®-treatment group. Lymphocyte count for infected untreated was non-significantly higher than for the uninfected controls, but treatment with both trypanocides recorded further increases, which were higher compared with that of the uninfected group. Post infection and treatment, aspartate aminotransferase (AST) levels increased significantly. There were non-significant differences in electrolyte ion concentrations across the groups except for chloride ion which recorded a significant reduction in the Novidium®-treatment group. This experiment revealed that Nigerian donkeys infected with T. brucei brucei (Federe isolate) developed symptoms of trypanosomosis; anaemia, lymphocytosis and thrombocytopenia. Treatment with the trypanocides ameliorated effects of the infection, and results suggest that immunosuppression may not be a substantial clinical manifestation of T. brucei brucei (Federe isolate) trypanosomosis in Nigerian donkeys.

scholarly journals The Effect of Salam Leaf (Syzygium polyanthum [Wight] Walp.) Extract on Inflammation in Rat Model of Acute Coronary Syndrome

2020 ◽  
Vol 8 (A) ◽  
pp. 374-377
Author(s):  
Refli Hasan ◽  
Dharma Lindarto ◽  
Gontar Alamsyah Siregar ◽  
Zulfikri Mukhtar
Keyword(s):  
Treatment Group ◽  
Rat Model ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Macroscopic Appearance ◽  
Coronary Syndrome

AIM: The aim of the study was to determine the effect of Salam leaf extract on regenerating islet-derived protein 3 β (Reg3β) and C-reactive protein (CRP) in cardiomyocyte of rat model of ACS. METHODS: Thirty-six male Wistar rats were divided into three groups: Negative control group, positive control group (ACS-induced group), and treatment group (ACS-induced group with Salam leaf administration). Macroscopic appearance and histopathology examination were done to ensure the occurrence of infarction. Reg3β and CRP levels were measured and compared between the positive control group and treatment group. RESULTS: There were significantly lower Reg3β and CRP levels in the treatment group than in the positive control group from day 4 to day 14. CONCLUSION: These data suggest that a Salam leaf extract can improve inflammation by decreasing Reg3β and CRP levels in rat model of ACS.

scholarly journals Pengaruh Pemberian Ekstrak Kayu Manis Terhadap Gambaran Histopatologi Ginjal Pada Tikus Putih Jantan Yang Diinduksi Parasetamol

2021 ◽  
Vol 10 (2) ◽  
pp. 142
Author(s):  
Rachma Eka Rani ◽  
Fitri Handajani ◽  
Eva Pravitasari Nefertiti
Keyword(s):  
Single Dose ◽  
Treatment Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Cinnamon Extract ◽  
Significant Difference ◽  
Cinnamomum Burmannii

AbstrakLatar Belakang : Kayu manis (Cinnamomun burmannii) mengandung flavonoid dan sinamaldehid yang berguna sebagai antioksidan dan bersifat renal protektor. Parasetamol berguna sebagai antipiretik dan analgesik. Parasetamol dimetabolisme oleh sitokrom P450 di sel hepar membentuk glukoronida, sulfat, dan NAPQI. NAPQI merupakan hasil metabolit yang sangat reaktif dan mengakibatkan stres oksidatif. Penelitian ini bertujuan untuk engetahui efek pemberian ekstrak kayu manis  yang dapat mencegah kerusakan sel tubulus ginjal tikus putih jantan yang diinduksi parasetamol.Metode :  30 ekor tikus yang dibagi menjadi 3 kelompok; (1) kelompok kontrol negatif tanpa perlakuan, (2) kelompok kontrol positif yang diinduksi parasetamol dosis tunggal 1750 mg/kgBB pada hari ke 14, (3) kelompok perlakuan  yang diberi ekstrak kayu manis dosis 400 mg/kgBB selama 14 hari lalu diinduksi parasetamol dosis tunggal 1750 mg/kgBB pada hari ke 14. Pada hari ke 17  hewan coba dikorbankan, dilakukan pengambilan ginjal dan dipemeriksa secara mikroskopik. Selanjutnya dilakukan uji Kruskal-Wallis dan uji Mann-Whitney U.Hasil : Uji  Kruskal-Wallis menunjukkan terdapat perbedaan derajat kerusakan ginjal yang signifikan. Uji Mann-Whitney U menunjukkan perbedaan yang bermakna antara kelompok kontrol negatif dan kelompok kontrol positif p=0,001 (p<a); kelompok kontrol negatif dan kelompok perlakuan p=0,001 (p<a); juga antara kelompok kontrol positif dan kelompok perlakuan p=0,001 (p<a).Kesimpulan : Terdapat pengaruh pemberian ekstrak kayu manis gambaran histopatologi ginjal  tikus putih jantan yang diinduksi parasetamol.Kata Kunci : Ekstrak kayu manis (Cinnamomum burmannii), parasetamol, gambaran histopatologi ginjal AbstractBackground: Cinnamon (Cinnamomum burmannii) contains flavonoids and cinnamaldehyde which are useful as antioxidants and renal protectors. Paracetamol is useful as an antipyretic and analgesic. Paracetamol is metabolized by cytochrome P450 in liver cells to form glucuronide, sulfate, and NAPQI. NAPQI is the result of highly reactive metabolites and cause oxidative stress. This study aim to know the effect of cinnamon extract  which can prevent damage of renal tubular cell rats  male induced by paracetamol.Method: 30  rats used and divided into 3 groups (1) untreated group, (2) rats which were induced by single dose paracetamol 1750 mg/kgBW on 14th day, and  (3) rats were which given cinnamon extract dose 400 mg/kgBW for 14 days then induced single dose paracetamol 1750 mg/kgBW on day 14th. On the 17th day the kidneys are taken and microscopic examination is performed. Data analysis uses the Kruskal-Wallis test and the Mann-Whitney U test.Result: The Kruskal-Wallis test showed a significant difference in the degree of kidney damage. The Mann-Whitney U test showed a significant difference between the negative control group and the positive control group p=0.001 (p <); negative control group and the treatment group p=0.001 (p <); also between the positive control group and the treatment group p=0.001 (p <).Conclusion: There is an effect of administration of cinnamon extract  on the histopathological picture of the kidney of white rats male induced by paracetamol.Keyword:  Cinnamon extract (Cinnamomum burmannii), paracetamol, renal histopathology. 

scholarly journals The effectiveness of Boiled figs leaf (FIcus Carica L) and Rosy Periwinkle (Catharantus roseus) on SGOT and SGPT levels of male wistar strain rats with hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 850-859
Author(s):  
Debora Oktaviani
Keyword(s):  
Treatment Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups ◽  
Wistar Strain ◽  
Significant Difference

Hepatitis is an inflammatory disease of liver cells that is contagious in the low category, the cause of it is bacteria, viruses, drugs and alcohol. The purpose of this study was to determine the effectiveness of boiled figs leaf (Ficus Carica L) and Rosy periwinkle (Catharantus roseus ) in reducing SGOT and SGPT levels. The subjects of this study were 30 male Wistar strain rats with age of 2-3 months, body weight 180-200 grams. Rats were divided into 3 groups; treatment groups, negative control groups and positive control groups. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. The treatment group was given 0.4 grams boiled figs leaf (Ficus Carica L) and 2.6 grams boiled rosy periwinkle  (Catharantus roseus) it given as much as 3.6 cc / day orally for 7 days. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare SGOT and SGPT levels. The results showed there were significant differences in SGOT levels between the treatment group, the positive control group, and the negative control group (p <0.05) and there was a significant difference in the SGPT value between the treatment group and the positive control group (p <0.05), while the negative control group and the treatment group there was no significant difference (p = 0.75). The conclusion of this study was the water extraction of figs leaf (Ficus Carica L), Rosy periwinkle (Catharantus roseus) had an effect in decreasing the SGOT and SGPT values of male wistar strain rats induced by paracetamol.

scholarly journals The Effectiveness of Boiled Rosy Periwinkle (Catharanthus roseus) in Decreasing SGOT and SGPT Value of Male Wistar Strain Rats with Acute Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 780-791
Author(s):  
Rutdiana Zai ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Catharanthus Roseus ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Wistar Strain ◽  
Significant Difference

Acute hepatitis is a disease that attacks the liver and causes inflammation in the liver cells which is characterized by swelling, damage, death, and even disruption of liver function, so that it can result in increased values of Serum Glutamic Pyruvic Oxaloactic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT). This study aims to determine the effectiveness of the Rosy Periwinkle  (Catharanthus roseus) on decreasing SGOT and SGPT level. The object of this research is 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams which were adapted for 7 days. The object of this study was divided into 3 groups, namely the treatment group, the positive control group and the negative control group. The treatment group and positive control group were induced Paracetamol 120 mg / day orally for 7 days. 5.2 grams of boiled Rosy Periwinkle (Catharanthus roseus) as much as 3.6 cc orally for 7 days was given to the treatment group. Data were analyzed with SPSS version 24, One Way ANOVA test to compare SGOT and SGPT values. The results of this study found that there were a significant difference in the SGOT value between the treatment group, the positive control group and the negative control group (p <0.05) and there were significant differences in the SGPT value between the treatment group and the positive control group (p <0.05) . The conclusion of this study is that the boiled Rosy Periwinkle (Catharanthus roseus) has an effect on the decrease in SGOT and SGPT values in male wistar strain Rats with acute hepatitis model.

scholarly journals Cardioprotective effect of ethanolic extract of mengkudu (Morinda citrifolia) on rats induced by doxorubicin

2019 ◽  
Vol 4 (4) ◽  
pp. 7-12
Author(s):  
Lyvia Lovin ◽  
Nyoman Ehrich Lister I ◽  
Edy Fachrial ◽  
Sukirman Lie
Keyword(s):  
Treatment Group ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

Doxorubicin is an effective drug used in cancer therapy but produces reactive oxygen species (ROS) that are toxic to heart cells. The purpose of this study was to determine the cardioprotective activity of Mengkudu fruit ethanol extract against the heart induced by doxorubicin. Mengkudu fruit ethanol extract was obtained by maceration. Cardioprotective activity test is done by measuring blood LDH and CK-MB levels as well as cardiac histology. Animals were induced with DOX 5 mg/kg BW on day 1,7,14 and 20th. Administration of Mengkudu extract 100 mg / kg bw, 300 mg / kg bw, and 500 mg / kg BW given from day 1 to day 20 and on the 21st day cardiac serum levels of CK-MB normal group had a significant difference (p <0.05) with a negative control treatment group, treatment group I, treatment group II, and treatment group III and did not have a significant difference (P> 0.05) with a positive control group with vitamin e supplementation and serum LDH levels the normal group had a significant difference (p <0.05) with the negative control group, the treatment group I, the treatment group II, and the treatment group II and did not have a significant difference (P> 0.05) with the positive control group with vitamin e supplementation. Cardiology histology of the Mengkudu extract 100 mg/kg bw + DOX and the Mengkudu extract 300 mg/kg bb + DOX, and the negative control group showed bleeding, fragmentation and myocytolysis, in the treatment of group III, the group normal, and the positive control group did not show heart muscle damage. Based on the description above it can be concluded that the ethanol extract of Mengkudu fruit containing flavonoids has cardioprotective activity by inhibiting the formation of ROS. The higher the dose of an extract, the greater the decrease in LDH and CKMB levels and increase protection against heart damage.

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