scholarly journals Epidemiology and molecular characterization of Theileria annulata in cattle from central Khyber Pakhtunkhwa, Pakistan

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0249417
Author(s):  
Raqeeb Ullah ◽  
Sumaira Shams ◽  
Munsif Ali Khan ◽  
Sultan Ayaz ◽  
Noor ul Akbar ◽  
...  
Keyword(s):  
Risk Factors ◽  
18S Rrna ◽  
Tick Infestation ◽  
Theileria Annulata ◽  
Economic Losses ◽  
Host Age ◽  
Cattle Population ◽  
Tropical Theileriosis ◽  
Feeding Method ◽  
Khyber Pakhtunkhwa

Theileria annulata is a tick-borne hemoprotozoan parasite responsible for tropical theileriosis in the bovine population, which causes substantial economic losses to the livestock sector. The present study has investigated, characterized, and shaped epidemiologic and phylogenetic profiles of T. annulata infection in the cattle population of central Khyber Pakhtunkhwa, Pakistan. A total of 600 blood samples were collected from cattle. Microscopy and PCR (18S rRNA taxonomic marker) assays were performed to detect T. annulata infection in cattle from the study area. The overall relative prevalence rates of T. annulata in the examined cattle population were 12.8% (microscopy) and 23.7% (PCR). District-wise analysis (microscopy/PCR) showed that cattle from district Mardan were found more infected (16.0%/28.0%), as compared to cattle from district Charsadda (13.5%/25.5%) and district Peshawar (9.0%/17.5%). Based on host demographic and ecological parameters analysis, theileriosis was found to be higher in young, female, crossbred, freely grazing, tick-infested, and irregular/no acaricides treated cattle. The univariate logistic analysis showed that host age, tick infestation, acaricides use, and feeding method were significant risk factors (P<0.05) whereas multivariate analysis indicated that host age, gender, tick infestation, acaricidal application, and feeding method were potential risk factors (P<0.05) for tropical theileriosis in the cattle population. Phylogenetic and sequence analysis showed that T. annulata 18S rRNA isolates shared homology and phylogeny with other isolates from Asia and Europe. This study has addressed the epidemiology and phylogeny of T. annulata circulating in bovid in the study area where gaps were still present. These findings will serve as a baseline and will facilitate future large-scale epidemiological investigations on tropical theileriosis in the cattle population at a national level.

Molecular epidemiology, risk factors and hematochemical alterations induced by Theileria annulata in bovines of Punjab (India)

2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Ashuma Tuli ◽  
Lachhman Das Singla ◽  
Amrita Sharma ◽  
Mandeep Singh Bal ◽  
Gursimran Filia ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Theileria Annulata ◽  
Rrna Gene ◽  
Tropical Theileriosis ◽  
Indigenous Cattle ◽  
Cross Sectional ◽  
Blood Smear Examination ◽  
Dairy Animals ◽  
Theileria Spp

AbstractBovine tropical theileriosis, caused by Theileria annulata, is one of the economically important fatal tick borne haemoprotozoan diseases of dairy animals. The aim of present investigation was to map the distribution of T. annulata in bovines of Punjab state of India in relation to various risk factors including age, sex of animals, location and management of farms. In a cross sectional study, a total of 1278 blood samples were randomly collected from twenty districts falling in five major agro-climatic zones of Punjab. All the samples were screened by blood smear examination followed by polymerase chain reaction targeting SSU rRNA gene for Theileria spp. PCR positive samples (n = 386) for Theileria spp. were then analyzed for T. annulata by amplification of Tams1 gene. Overall prevalence of T. annulata was found to be 29.26% in Punjab, with highest in western Zone (40.49%, 95% CI = 35.57-45.41) and lowest in submountain zone (18.90%, 95% CI = 13.73-24.06). The propensity of incidence of T. annulata was found to be highest in cross bred cattle (32.40%, 95% CI = 29.87-34.94), followed by indigenous cattle (19.64%, 95% CI = 10.67-28.61) and buffaloes (19.2%, 95% CI = 14.99-23.41). Between the two sexes, incidence of T. annulata was higher in female animals. Calves less than 6 months of age were found to be more prone to theileriosis.

scholarly journals Risk factors associated with tick infestations on equids in Khyber Pakhtunkhwa, Pakistan, with notes on Rickettsia massiliae detection

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Abid Ali ◽  
Hafsa Zahid ◽  
Ismail Zeb ◽  
Muhammad Tufail ◽  
Sulaiman Khan ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dna Sequences ◽  
Tick Species ◽  
Tick Infestation ◽  
Morphological Identification ◽  
Rrna Gene ◽  
Spotted Fever Group ◽  
Khyber Pakhtunkhwa ◽  
Rickettsia Massiliae ◽  
Associated Risk Factors

Abstract Background Studies on ticks infesting equids are lacking in various parts of the world, including Khyber Pakhtunkhwa (KP), Pakistan. The aim of this study was to investigate the diversity of ticks infesting equids, associated risk factors and rickettsial detection in ticks from equids in KP. Methods Inspection of 404 equid hosts from November 2018 to October 2019 resulted in the collection of 550 ticks. Data on tick-associated risk factors were collected from equid owners by means of a questionnaire. After morphological identification, partial DNA sequences of the tick mitochondrial 16S rRNA gene were used for taxonomic confirmation of species. Partial sequences of the gltA and ompA genes were used for Rickettsia detection in ticks. Results A total of 550 tick specimens were collected on 324 (80.2%) of the equids inspected, of which 161 were horses (50%), 145 (45%) were donkeys and 18 were mules (5%). The ticks were identified as belonging to the following five species: Rhipicephalus microplus (341 specimens, 62% of the total ticks), Rh. haemaphysaloides (126, 23%), Rh. turanicus (39, 7%), Rh. sanguineus (s.l.) (33, 6%) and Hyalomma anatolicum (11, 2%). The most prevalent tick life stage was adult females (279, 51%) followed by adult males (186, 34%) and nymphs (85, 15%). Higher tick infestations were observed on male equids (relative risk [RR] 0.7432, P < 0.0005) and adult equids (RR 1.268, P < 0.0020). Ticks were frequently attached to the axial region of horses (55, 21%), sternum of donkeys (44, 21%) and belly of mules (19, 23%) (P < 0.04). Temporal patterns of tick infestation in association with temperature and humidity were highly significant (P < 0.05). Risk factors, such as animal housing (P < 0.0003), living management (P < 0.006), grazing type (P < 0.01) and location in hilly areas (P < 0.02), significantly enhanced the chances for tick infestation. Tick species analyzed in this study were phylogenetically related to species from Afghanistan, China, South Africa and Taiwan. Partial sequences of the gltA and ompA genes obtained from Rh. microplus and Rh. haemaphysaloides were 100% identical to the spotted fever group pathogen Rickettsia massiliae. Conclusions Equids exposed to significant risk factors were infected by one or more of at least five tick species in KP, Pakistan, and some of the ticks harbored the human pathogen R. massiliae. Graphical abstract

scholarly journals Discovery of a new Theileria sp. closely related to Theileria annulata in cattle from Sri Lanka

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Thillaiampalam Sivakumar ◽  
Shiori Fujita ◽  
Bumduuren Tuvshintulga ◽  
Hemal Kothalawala ◽  
Seekkuge Susil Priyantha Silva ◽  
...  
Keyword(s):  
Sri Lanka ◽  
18S Rrna ◽  
Surface Protein ◽  
Theileria Annulata ◽  
Sri Lankan ◽  
Nucleotide Polymorphisms ◽  
Gene Sequences ◽  
Tropical Theileriosis ◽  
Single Nucleotide ◽  
Theileria Lestoquardi

Abstract Theileria annulata is a haemoprotozoan parasite that causes a cancer-like illness known as tropical theileriosis in cattle. In the course of analyzing the genetic diversity of T. annulata in Sri Lanka, we observed that merozoite-piroplasm surface antigen (tams1) and surface protein (tasp)-like gene sequences obtained from bovine blood DNA samples, which were PCR-positive for T. annulata, were conserved but shared low identity with T. annulata GenBank sequences. Moreover, the 18S rRNA sequences from the Sri Lankan samples contained ten unique single-nucleotide polymorphisms compared with all known T. annulata sequences. The cytochrome b (cob) gene sequences isolated from the Sri Lankan samples were highly conserved and shared low identity scores with similarly conserved T. annulata sequences from GenBank. Phylogenetic analysis showed that the Sri Lankan tams1-like, tasp-like, 18S rRNA, and cob sequences clustered together and formed sister clades to the common ancestors of all known T. annulata and Theileria lestoquardi sequences. These findings demonstrated that the Sri Lankan cattle were not infected with T. annulata but with a new Theileria sp. (designated as Theileria sp. Yokoyama) closely related to T. annulata.

scholarly journals Prevalence of BRD-Related Viral Pathogens in the Upper Respiratory Tract of Swiss Veal Calves

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1940
Author(s):  
Eveline Studer ◽  
Lutz Schönecker ◽  
Mireille Meylan ◽  
Dimitri Stucki ◽  
Ronald Dijkman ◽  
...  
Keyword(s):  
Risk Factors ◽  
Welfare Economic ◽  
Economic Losses ◽  
Nasopharyngeal Swab ◽  
Upper Respiratory Tract ◽  
Convenience Sample ◽  
Veal Calves ◽  
Mixed Regression ◽  
Influenza C Virus ◽  
Syncytial Virus

The prevention of bovine respiratory disease is important, as it may lead to impaired welfare, economic losses, and considerable antimicrobial use, which can be associated with antimicrobial resistance. The aim of this study was to describe the prevalence of respiratory viruses and to identify risk factors for their occurrence. A convenience sample of 764 deep nasopharyngeal swab samples from veal calves was screened by PCR for bovine respiratory syncytial virus (BRSV), bovine parainfluenza-3 virus (BPI3V), bovine coronavirus (BCoV), influenza D virus (IDV), and influenza C virus (ICV). The following prevalence rates were observed: BRSV, 2.1%; BPI3V, 3.3%; BCoV, 53.5%; IDV, 4.1%; ICV, 0%. Logistic mixed regression models were built for BCoV to explore associations with calf management and housing. Positive swab samples were more frequent in younger calves than older calves (>100 days; p < 0.001). The probability of detecting BCoV increased with increasing group size in young calves. Findings from this study suggested that young calves should be fattened in small groups to limit the risk of occurrence of BCoV, although an extended spectrum of risk factors for viral associated respiratory disorders such as nutritional aspects should be considered in future studies.

scholarly journals Sero-epidemiological survey of bluetongue disease in one-humped camel (Camelus dromedarius) in Kassala State, Eastern Sudan

2021 ◽  
Vol 74 (1) ◽  
Author(s):  
Molhima M. Elmahi ◽  
Mohammed O. Hussien ◽  
Abdel Rahim E. Karrar ◽  
Amira M. Elhassan ◽  
Abdel Rahim M. El Hussein
Keyword(s):  
Risk Factors ◽  
Potential Risk ◽  
Animal Health ◽  
Water Source ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Cross Sectional ◽  
Potential Risk Factors ◽  
Eastern Sudan

Abstract Background Bluetongue (BT) is a vector-borne viral disease of ruminant and camelid species which is transmitted by Culicoides spp. The causative agent of BT is bluetongue virus (BTV) that belongs to genus Orbivirus of the family Reoviridae. The clinical disease is seen mainly in sheep but mostly sub-clinical infections of BT are seen in cattle, goats and camelids. The clinical reaction of camels to infection is usually not apparent. The disease is notifiable to the World Organization for Animal Health (OIE), causing great economic losses due to decreased trade and high mortality and morbidity rates associated with bluetongue outbreaks. The objective of this study was to investigate the seroprevalence of BTV in camels in Kassala State, Eastern Sudan and to identify the potential risk factors associated with the infection. A cross sectional study using a structured questionnaire survey was conducted during 2015–2016. A total of 210 serum samples were collected randomly from camels from 8 localities of Kassala State. The serum samples were screened for the presence of BTV specific immunoglobulin (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (cELISA). Results Seropositivity to BTV IgG was detected in 165 of 210 camels’ sera accounting for a prevalence of 78.6%. Potential risk factors to BTV infection were associated with sex (OR = 0.061, p-value = 0.001) and seasonal river as water source for drinking (OR = 32.257, p-value = 0.0108). Conclusions Sex and seasonal river as water source for drinking were considered as potential risk factors for seropositivity to BTV in camels. The high prevalence of BTV in camels in Kassala State, Eastern Sudan, necessitates further epidemiological studies of BTV infection in camels and other ruminant species to better be able to control BT disease in this region.

scholarly journals Mapping, cluster detection and evaluation of risk factors of ovine toxoplasmosis in Southern Italy

2016 ◽  
Vol 11 (2) ◽  
Author(s):  
Roberto Condoleo ◽  
Vincenzo Musella ◽  
Maria Paola Maurelli ◽  
Antonio Bosco ◽  
Giuseppe Cringoli ◽  
...  
Keyword(s):  
Risk Factors ◽  
Geographical Information Systems ◽  
Southern Italy ◽  
Geographical Information ◽  
Economic Losses ◽  
Common Source ◽  
High Exposure ◽  
Campania Region ◽  
Source Of Infection ◽  
Serological Data

Toxoplasmosis, an important cause of reproductive failure in sheep, is responsible for significant economic losses to the ovine industry worldwide. Moreover, ovine meat contaminated by the parasite <em>Toxoplasma gondii</em> is considered as a common source of infection for humans. The aim of this study was to develop point and risk profiling maps of <em>T. gondii</em> seroprevalence in sheep bred in Campania Region (Southern Italy) and analyse risk factors associated at the flock-level. We used serological data from a previous survey of 117 sheep flocks, while environmental and farm management information were obtained from an analysis based on geographical information systems and a questionnaire purveyance, respectively. An univariate Poisson regression model revealed that the type of farm production (milk and meat vs only meat) was the only independent variable associated with <em>T. gondii</em> positivity (P&lt;0.02); the higher within-flock seroprevalence in milking herds suggests that milking practices might influence the spread of the infection on the farm. Neither environmental nor other management variables were significant. Since a majority of flocks were seasonally or permanently on pasture, the animals have a high exposure to infectious <em>T. gondii</em> oocysts, so the high within-flock seroprevalence might derive from this management factor. However, further studies are needed to better assess the actual epidemiological situation of toxoplasmosis in sheep and to clarify the factors that influence its presence and distribution.

scholarly journals Prevalence and herd-level risk factors of bovine tuberculosis in the State of Goiás, Brazil

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3625 ◽  
Author(s):  
Willian Vilela Rocha ◽  
Valéria De Sá Jayme ◽  
Ana Lourdes Arrais de Alencar Mota ◽  
Wilia Marta Elsner Diederichsen de Brito ◽  
Glauciane Ribeiro de Castro Pires ◽  
...  
Keyword(s):  
Risk Factors ◽  
Bovine Tuberculosis ◽  
Protective Factor ◽  
Management Practices ◽  
Multiple Logistic Regression Analysis ◽  
Livestock Production ◽  
The State ◽  
Economic Losses ◽  
Strong Concentration ◽  
Herd Level

Bovine tuberculosis results in economic losses in livestock production and poses a risk to public health. This study aimed to characterize the epidemiological status of bovine tuberculosis in adult cows in the state of Goiás, Brazil. The state was divided into three sampling strata, corresponding to different livestock production circuits. A total of 18,659 animals were tested with the comparative cervical tuberculin test (CCT), in 300 randomly sampled bovine herds per stratum. An epidemiological questionnaire was used to identify health and management practices that may be associated with the presence of tuberculosis infection in the herd. In Stratum 1, dominated by beef cattle, no animals reacted to the CCT. In Stratum 2, which covers the main dairy regions of the state, the prevalence was estimated at 8.67% [5.73 to 12.74%] for herds and at 0.9% [0.21 to 1.58%] for animals. In Stratum 3, characterized by a mix of dairy, beef and dual-purpose herds, the prevalence was estimated at 1.00% [0.21 to 2.89] for herds and 0.30% [0.10 to 0.49 %] for animals. The overall prevalence in the state of Goiás was 3.43% [2.20 to 4.67%] for herds and 0.30% [0.10 to 0.49%] for animals. The multiple logistic regression analysis revealed that herd-level risk factors associated with the presence of the bovine tuberculosis are: the location of the herd in Stratum 2 (OR = 12.05 [3.52 to 41.28]) and the number of times a cow is milked per day (OR = 6.27 [2.72 to 14.44]). Regular veterinary care was identified as a protective factor (OR = 0.38 [0.15-0.94]). These results indicate that bovine tuberculosis is endemic in the state; its spatial distribution is heterogeneous with a strong concentration in dairy regions. The most intensive dairy farms are those with the highest risk, which is consistent with what other authors’ found elsewhere in Brazil. The epidemiological information generated by this study provides information for planning of risk-based surveillance actions, and justifies the adoption of free-herd certification programs for bovine tuberculosis in the main dairy regions of Goiás.

scholarly journals Sero-Prevalence of Contagious Caprine Pleuropneumonia in Goats in Afgoye District Lower Shabelle Region, Somalia

2020 ◽  
Vol 5 (2) ◽  
pp. 1-4
Author(s):  
Omar Mohamed Abdi
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Latex Agglutination ◽  
Economic Losses ◽  
High Morbidity ◽  
Cross Sectional ◽  
Mycoplasma Capricolum ◽  
Contagious Caprine Pleuropneumonia ◽  
Epidemiological Risk ◽  
Associated Risk Factors

Background: Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. Capripneumoniae (Mccp), is one of the most important goat diseases causing high morbidity and mortality, these results in heavy economic losses to countries like Somalia, which is a country affected the civil war of the 1990s. Objective: Generally, the disease presence has been reported in the country. However, there is no quantitative data on the prevalence and distribution of the disease has been scientifically reported in the country. Methods: A cross-sectional study was conducted from September 2019 to June 2020 in three villages of Afgoye district of Somalia to assess the seroprevalence and identify the associated risk factors for the occurrence of the CCPP. The risk factors considered included sex, age, and locations. Results: A total of 100 blood samples were collected and evaluated by Capri-Latex Agglutination Test (Capri-LAT). A total of 49/100 (49%, CI 95%: (38.86%-59.2%) goats were seropositive for anti-CCPP antibody. The epidemiological risk factors considered, sex, age and locations, were not significantly associated with the CCPP sero prevalence (p>0.05). However, the sero positivity was slightly higher in female (51%, 95% CI: 39.8-62.6), >3 years old goats (86.3%, 95% CI: 48.9-87.4) and Markazka village (56%, 95% CI: 41.3-70.0) compared to male (40%, 95% CI: 19.1-63.9), 1-3 years old (57.5%, 95% CI: 40.9- 73.0), and Shareeco (43.8%, 95% CI: 19.8-70.1) and Siinka (41.2%, 95% CI: 24.7-59.3) villages, respectively. Conclusion: The present study indicates the prevalence of CCPP in the studies areas and this requires practicable measures to be put in place towards the control of the disease.

scholarly journals Prevalence and Risk Factors Analysis for Cryptosporidium in Apparently Healthy Lambs of Southern Khyber Pakhtunkhwa, Pakistan

2018 ◽  
Vol 50 (3) ◽  
Author(s):  
Naimat Ullah Khan ◽  
Muhammad Hassan Saleem ◽  
Aneela Zameer Durrani ◽  
Nisar Ahmad ◽  
Ayesha Hassan ◽  
...  
Keyword(s):  
Risk Factors ◽  
Khyber Pakhtunkhwa ◽  
Factors Analysis ◽  
Apparently Healthy

scholarly journals Identification of risk factors for human immunodeficiency virus-1 infection in Khyber Pakhtunkhwa population: A case control study

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Jamila Haider ◽  
Ghosia Lutfullah ◽  
Irshad Ur Rehman ◽  
Irfan Khattak
Keyword(s):  
Risk Factors ◽  
Human Immunodeficiency Virus ◽  
Case Control Study ◽  
Case Control ◽  
Male Gender ◽  
Khyber Pakhtunkhwa ◽  
Immunodeficiency Virus ◽  
History Of ◽  
Control Study ◽  
Human Immunodeficiency Virus 1

Objectives: The present study aims to identify the risk factors for Human Immunodeficiency Virus-1(HIV-1) infection in Khyber Pakhtunkhwa (KP) population by comparing HIV-antibody positive cases with HIV-antibody-negative controls. Methods: The study was designed at the Family Care Centre (FCC), Hayatabad Medical Centre (HMC) Peshawar from February 2015 to December 2016. A total of 280 individuals were selected randomly for the study as cases and control. Data was collected on a structured questionnaire with informed oral consent. The collected data was analysed statistically using SPSS version 20. Results: Out of 280 individuals, 56% were males, 44% were females, and 53.21% belonged to the urban areas. The literacy rate was 48.6%, and 75.4% were married. The statistical analysis of risk factors revealed the following factors as of significance value (p < 0.05). Family history of HIV (OR = 9.46), spouse status of HIV (OR=22.22), injection drug users (IDUs), migrants (OR=2.234), use of therapeutic injections (OR= 2.791), employment (OR=2.545), male gender (OR=2.35), tattooing (OR=7.667) and history of blood transfusion (OR= 2.69). Conclusion: The present study revealed spouse status of HIV, tattooing, migrants, IDUs, use of therapeutic injections, history of blood transfusion, male gender and employment as significant risk factors for HIV infection in the population of KP. doi: https://doi.org/10.12669/pjms.35.5.258 How to cite this:Haider J, Lutfullah G, Irshad ur Rehman, Khattak I. Identification of risk factors for human immunodeficiency virus-1 infection in Khyber Pakhtunkhwa population: A case control study. Pak J Med Sci. 2019;35(5):---------. doi: https://doi.org/10.12669/pjms.35.5.258 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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