scholarly journals Molecular characterization of Cryptosporidium spp. in dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially zoonotic species and genotype

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255087
Author(s):  
Amanda Gleyce Lima de Oliveira ◽  
Adriana Pittella Sudré ◽  
Teresa Cristina Bergamo do Bomfim ◽  
Helena Lúcia Carneiro Santos
Keyword(s):  
Diarrheal Disease ◽  
Close Contact ◽  
Companion Animals ◽  
Protozoan Parasite ◽  
Health Concern ◽  
Fecal Samples ◽  
Glycoprotein Gene ◽  
Low Prevalence ◽  
The City

Intestinal cryptosporidiosis is a diarrheal disease caused by protists of genus Cryptosporidium that infect a wide variety of hosts, primarily vertebrates. Due to the close contact between humans and their companion animals, especially dogs and cats, there is concern about the potential for zoonotic transmission of this enteric protozoan parasite by infected animals. This study aimed to perform a microscopic and molecular diagnosis of Cryptosporidium spp. in fecal samples from domiciled dogs and cats. One hundred and nineteen fecal samples were processed using sugar centrifugal flotation followed by molecular detection of Cryptosporidium spp. DNA using nested PCR. Subtyping of isolates positive for C. parvum was performed by sequence analysis of the 60 kDa glycoprotein gene (GP60). Cryptosporidium oocysts were detected in 7.8% (5/64) and 5.4% (3/55) of the fecal samples from dogs and cats, respectively. Cryptosporidium canis (n = 3) and C. parvum (n = 2) were the main species found in dogs, whereas C. felis (n = 3) was prevalent in cats. Subtype IIaA17G2R2 (potentially zoonotic) was identified in samples positive for C. parvum. Despite the low prevalence of Cryptosporidium observed in the domiciled dogs and cats, the presence of potentially zoonotic C. parvum in dogs evidences a public health concern. Further research is needed to better understand the epidemiology, source, and potential impacts of Cryptosporidium infection in cats and dogs.

scholarly journals High Diversity of Cryptosporidium Species and Subtypes Identified in Cryptosporidiosis Acquired in Sweden and Abroad

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 523
Author(s):  
Marianne Lebbad ◽  
Jadwiga Winiecka-Krusnell ◽  
Christen Rune Stensvold ◽  
Jessica Beser
Keyword(s):  
Cryptosporidium Parvum ◽  
Ribosomal Rna ◽  
Diarrheal Disease ◽  
Protozoan Parasite ◽  
Small Subunit ◽  
Cryptosporidium Species ◽  
Intestinal Protozoan ◽  
Genotype I ◽  
Glycoprotein Gene ◽  
High Diversity

The intestinal protozoan parasite Cryptosporidium is an important cause of diarrheal disease worldwide. The aim of this study was to expand the knowledge on the molecular epidemiology of human cryptosporidiosis in Sweden to better understand transmission patterns and potential zoonotic sources. Cryptosporidium-positive fecal samples were collected between January 2013 and December 2014 from 12 regional clinical microbiology laboratories in Sweden. Species and subtype determination was achieved using small subunit ribosomal RNA and 60 kDa glycoprotein gene analysis. Samples were available for 398 patients, of whom 250 (63%) and 138 (35%) had acquired the infection in Sweden and abroad, respectively. Species identification was successful for 95% (379/398) of the samples, revealing 12 species/genotypes: Cryptosporidium parvum (n = 299), C. hominis (n = 49), C. meleagridis (n = 8), C. cuniculus (n = 5), Cryptosporidium chipmunk genotype I (n = 5), C. felis (n = 4), C. erinacei (n = 2), C. ubiquitum (n = 2), and one each of C. suis, C. viatorum, C. ditrichi, and Cryptosporidium horse genotype. One patient was co-infected with C. parvum and C. hominis. Subtyping was successful for all species/genotypes, except for C. ditrichi, and revealed large diversity, with 29 subtype families (including 4 novel ones: C. parvum IIr, IIs, IIt, and Cryptosporidium horse genotype VIc) and 81 different subtypes. The most common subtype families were IIa (n = 164) and IId (n = 118) for C. parvum and Ib (n = 26) and Ia (n = 12) for C. hominis. Infections caused by the zoonotic C. parvum subtype families IIa and IId dominated both in patients infected in Sweden and abroad, while most C. hominis cases were travel-related. Infections caused by non-hominis and non-parvum species were quite common (8%) and equally represented in cases infected in Sweden and abroad.

scholarly journals Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amy Gaye ◽  
Tolla Ndiaye ◽  
Mouhamad Sy ◽  
Awa B. Deme ◽  
Alphonse B. Thiaw ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Clinical Signs ◽  
Health Concern ◽  
Public Health Concern ◽  
Local Circulation ◽  
Viral Genomes ◽  
Genomic Characterization ◽  
The City

AbstractDengue virus is a major and rapidly growing public health concern in tropic and subtropic regions across the globe. In late 2018, Senegal experienced its largest dengue virus outbreak to date, covering several regions. However, little is known about the genetic diversity of dengue virus (DENV) in Senegal. Here we report complete viral genomes from 17 previously undetected DENV cases from the city of Thiès. In total we identified 19 cases of DENV in a cohort of 198 individuals with fever collected in October and November 2018. We detected 3 co-circulating serotypes; DENV 3 was the most frequent accounting for 11/17 sequences (65%), 4 (23%) were DENV2 and 2 (12%) were DENV1. Sequences were most similar to recent sequences from West Africa, suggesting ongoing local circulation of viral populations; however, detailed inference is limited by the scarcity of available genomic data. We did not find clear associations with reported clinical signs or symptoms, highlighting the importance of testing for diagnosing febrile diseases. Overall, these findings expand the known range of DENV in Senegal, and underscore the need for better genomic characterization of DENV in West Africa.

scholarly journals Isolation and Characterization of Coronavirus and Rotavirus Associated With Calves, in Central Part of Oromia, Ethiopia

2020 ◽  
Author(s):  
Umer Seid Geletu ◽  
Fufa Dawo Bari ◽  
Munera Ahmednur Usmael ◽  
Asamino Tesfaye
Keyword(s):  
Diarrheal Disease ◽  
Addis Ababa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infection Prevalence ◽  
Rt Pcr ◽  
Cross Sectional ◽  
Fecal Samples ◽  
Rotavirus Infection ◽  
Isolation And Characterization

Abstract Background: Coronavirus and Rotavirus are most commonly associated etiologies for calves’ diarrhea resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by coronavirus and rotavirus in calves in Ethiopia are inadequately understood. A cross sectional study was conducted with the aim of isolation and molecular characterization of coronavirus and rotavirus from calves in central part of Oromia (Bishoftu, Sebata, Holeta and Addis Ababa), Ethiopia from November 2018 to May 2019. The four study areas were purposively selected and fecal samples were collected by simple random sampling for diagnosis of coronavirus and rotavirus infection by using antigen detection Enzyme linked immunosorbent assay (Ag-ELISA) kit. In addition, this study was carried out to have insight in prevalence and associated risk factors of coronavirus and rotavirus infection in calves. Result: During the study 83 diarrheic and 162 non-diarrheic fecal samples collected from calves less than 4 weeks of age were screened for coronavirus and rotavirus. Of the 83 diarrheic samples, 1 sample (1.2%) was positive for coronavirus antigen (Ag) and 6 samples (7.2%) were found to be positive for rotavirus antigen (Ag) by Ag-ELISA. All the non-diarrheic samples were negative for both coronavirus and rotavirus Ag. The overall prevalence of coronavirus and rotavirus infection in calves were estimated as 0.4% (1/245) and 2.45% (6/245) respectively. All samples (7) of ELISA test positive of both coronavirus and rotavirus were propagated in Madin Darby bovine kidney cells (MDBK). After 3 subsequent passages, progressive cytopathic effect (CPE) i.e. rounding, detachment as well as destruction of mono-layer cell of five sample (1 sample of coronavirus and 4 sample of rotavirus) (71.4%) were observed. At the molecular stage, reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to determine the presence of coronavirus and rotavirus nucleic acid by using specific primers. The 5 samples that were coronavirus and rotavirus antigen positive by ELISA and develop CPE on cell culture were also positive on RT-PCR technique. Infection prevalence peaked have been obtained at 1st and 2nd weeks of age in male calves. Conclusion: Diarrheal disease caused by coronavirus and rotavirus has a great health problem in calves that interrupts production benefits with reduced weight gain and increased mortality, and its potential for zoonotic spread. So the present findings show coronavirus and rotavirus infection in calves in Ethiopia that needs to be addressed by practicing early colostrums feeding in newborn calves, using vaccine, or improving livestock management.

scholarly journals Parasitic Nematode and Protozoa Status of Working Sheepdogs on the North Island of New Zealand

Animals ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 94 ◽  
Author(s):  
Adam O’Connell ◽  
Ian Scott ◽  
Naomi Cogger ◽  
Boyd Jones ◽  
Kate Hill
Keyword(s):  
New Zealand ◽  
Close Contact ◽  
Parasite Burden ◽  
Protozoan Parasite ◽  
Gastrointestinal Nematode ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Fecal Samples ◽  
The North ◽  
Anthelmintic Drugs

Working farm dogs in New Zealand may have a high parasitic challenge because of access to raw meat and close contact with other dogs. This cross-sectional study aimed to estimate the percentage of dogs with gastrointestinal nematode and protozoan parasite lifecycle stages present in their feces and to identify factors associated with the presence of parasites. A single researcher collected information about the dogs and their management via a questionnaire, body condition scored (BCS) the dogs, and collected fecal samples to determine the parasite burden. Fecal samples were collected from 171 dogs and 40% (95% CI 33.0% to 47.7%) contained parasite ova or (oo)cysts. There was no association between BCS and the presence of nematodes and parasites (p = 0.74) in the feces. The percentage of dogs with parasites present in their feces was not associated with BCS or the frequency with which anthelmintic drugs were reportedly administered (p = 0.61). The high percentage of dogs with parasites are of concern for the health of the dogs and their owners, given the zoonotic potential of some parasites. Further, research should also focus on understanding why reporting giving anthelmintic drugs at least every three months did not eliminate the infection.

scholarly journals Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018

2020 ◽  
Author(s):  
Amy Gaye ◽  
Tolla Ndiaye ◽  
Mouhamad Sy ◽  
Awa B. Deme ◽  
Alphonse B. Thiaw ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Clinical Signs ◽  
Health Concern ◽  
Metagenomic Sequencing ◽  
Local Circulation ◽  
Genomic Characterization ◽  
Virus Genomes ◽  
The City

AbstractDengue virus is a major and rapidly growing public health concern in tropic and subtropic regions across the globe. In late 2018, Senegal experienced its largest dengue virus outbreak to date, covering several regions. However, little is known about the genetic diversity of dengue virus (DENV) in Senegal. Here we used molecular tools including metagenomic sequencing to identify 19 previously undetected dengue virus cases from the city of Thiès and assemble 17 complete virus genomes. DENV3 was the most frequent serotype; 11 sequences (65%) were DENV3, 4 sequences were DENV2 and 2 were DENV1. Sequences were closest to recent sequences from West Africa, suggesting ongoing local circulation of viral populations; however, detailed inference is limited by the scarcity of available genomic data. We did not find clear associations with reported clinical signs or symptoms, highlighting the importance of testing for diagnosing febrile diseases. Overall, these findings expand the known range of DENV in Senegal, and underscore the need for better genomic characterization of DENV in West Africa.

scholarly journals Quantification and Characterization of Metals in Ultrafine Road Dust Particles

Atmosphere ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1564
Author(s):  
Suzanne Beauchemin ◽  
Christine Levesque ◽  
Clare L. S. Wiseman ◽  
Pat E. Rasmussen
Keyword(s):  
Ultrafine Particle ◽  
Road Dust ◽  
Health Concern ◽  
Dust Particles ◽  
Lung Cells ◽  
Particle Size Fractions ◽  
Icp Ms ◽  
Local Roads ◽  
The City

Road dust is an important source of resuspended particulate matter (PM) but information is lacking on the chemical composition of the ultrafine particle fraction (UFP; <0.1 µm). This study investigated metal concentrations in UFP isolated from the “dust box” of sweepings collected by the City of Toronto, Canada, using regenerative-air-street sweepers. Dust box samples from expressway, arterial and local roads were aerosolized in the laboratory and were separated into thirteen particle size fractions ranging from 10 nm to 10 µm (PM10). The UFP fraction accounted for about 2% of the total mass of resuspended PM10 (range 0.23–8.36%). Elemental analysis using ICP-MS and ICP-OES revealed a marked enrichment in Cd, Cr, Zn and V concentration in UFP compared to the dust box material (nano to dust box ratio ≥ 2). UFP from arterial roads contained two times more Cd, Zn and V and nine times more Cr than UFP from local roads. The highest median concentration of Zn was observed for the municipal expressway, attributed to greater volumes of traffic, including light to heavy duty vehicles, and higher speeds. The observed elevated concentrations of transition metals in UFP are a human health concern, given their potential to cause oxidative stress in lung cells.

scholarly journals Genotypes of Giardia duodenalis in Household Dogs and Cats from Poland

2020 ◽  
Author(s):  
Agnieszka Piekara-Stępińska ◽  
Jolanta Piekarska ◽  
Michał Gorczykowski ◽  
Jacek Bania
Keyword(s):  
Companion Animals ◽  
Giardia Duodenalis ◽  
Protozoan Parasite ◽  
Zoonotic Potential ◽  
Current Frequency ◽  
Fecal Samples ◽  
Pet Dogs ◽  
One Year

Abstract Background Giardia duodenalis is a widespread protozoan parasite affecting humans and many species of animals, including dogs and cats. Due to its zoonotic potential, it is important to know the frequency of this parasite in companion animals. The aim of this study was to determine current epidemiological status of G. duodenalis in household dogs and cats. Methods In this study, 293 fecal samples from pet dogs and cats were collected from January 2017 to July 2019 and tested for G. duodenalis by PCR (using β-giardin gene). The animals were divided into groups depending on their age, breed and fecal consistency. Results The examination allowed for detection of G. duodenalis in 6.0% of canine and 3.9% of feline fecal samples. The highest frequency was revealed in young (under one-year old) dogs. Sequencing confirmed the presence of assemblages C and D in dogs and A and F in cats. Conclusion The study showed current frequency of G. duodenalis in dogs and cats and also revealed the occurrence of host-specific assemblages as well as zoonotic assemblage A.

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Comparação de testes coproparasitológicos em criadouro comercial de psitacídeos exóticos

2020 ◽  
Vol 3 (5) ◽  
pp. 100-107
Author(s):  
Marina Camargo de Sousa ◽  
◽  
Julia Ronzani Vial ◽  
Rodrigo Hidalgo Friciello Teixeira ◽  
Andrea Cristina Higa Nakaghi ◽  
...  
Keyword(s):  
Statistical Tests ◽  
Companion Animals ◽  
Clinical Importance ◽  
Breeding Site ◽  
Chi Square ◽  
Fecal Samples ◽  
Breeding Sites ◽  
Specificity And Sensitivity ◽  
Nematode Eggs ◽  
Commercial Breeding

Birds of the psittaciform order, composed by the Psittacidae and Loridae family have several characteristics making them more frequently kept as companion animals, promoting the increase of breeding sites in Brazil. The present study aimed to analyze the specificity and sensitivity of three different coproparasitological tests, Willis, Hoffman and Direto de feces, through statistical tests: Chi-Square and Kappa. 70 fecal samples of exotic parrots were collected from a commercial breeding site and these were submitted to the three tests, totaling 210 coproparasitological exams. Among the tests performed, 29,5% were positive for nematode eggs, cestodes and oocysts. Coproparasitological exams are inexpensive, have clinical importance, indicating the population of endoparasites and therapeutic treatments.

SARS-COV2 virus and Coronavirus disease (COVID-19)

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 977-982
Author(s):  
Mohamed J. Saadh ◽  
Bashar Haj Rashid M ◽  
Roa’a Matar ◽  
Sajeda Riyad Aldibs ◽  
Hala Sbaih ◽  
...  
Keyword(s):  
Close Contact ◽  
Antiviral Agents ◽  
Health Concern ◽  
The Novel ◽  
Global Public Health ◽  
Fatal Disease ◽  
Mild Disease ◽  
Life Threatening ◽  
Novel Coronavirus

SARS-COV2 virus causes Coronavirus disease (COVID-19) and represents the causative agent of a potentially fatal disease that is of great global public health concern. The novel coronavirus (2019) was discovered in 2019 in Wuhan, the market of the wet animal, China with viral pneumonia cases and is life-threatening. Today, WHO announces COVID-19 outbreak as a pandemic. COVID-19 is likely to be zoonotic. It is transmitted from bats as intermediary animals to human. Also, the virus is transmitted from human to human who is in close contact with others. The computerized tomographic chest scan is usually abnormal even in those with no symptoms or mild disease. Treatment is nearly supportive; the role of antiviral agents is yet to be established. The SARS-COV2 virus spreads faster than its two ancestors, the SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV), but has lower fatality. In this article, we aimed to summarize the transmission, symptoms, pathogenesis, diagnosis, treatment, and vaccine to control the spread of this fatal disease.

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