scholarly journals Special aspects of systemic inflammation course in animals

2019 ◽  
Vol 12 (7) ◽  
pp. 932-937 ◽  
Author(s):  
Svetlana Vladimirovna Chernigova ◽  
Yury Vladimirovich Chernigov ◽  
Yury Anatolyevich Vatnikov ◽  
Evgeny Vladimirovich Kulikov ◽  
Irina Anatolyevna Popova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Glutathione Reductase ◽  
Systemic Inflammation ◽  
Inflammatory Process ◽  
Unsaturated Fatty Acids ◽  
Control Group ◽  
Symptomatic Therapy ◽  
Membrane Structures ◽  
Pharmacological Agents ◽  
Septic Focus

Aim: In this study, we identified characteristics of systemic inflammation associated with surgical sepsis in animals. We evaluated the role of purine metabolism, functionally associated lipoperoxidation processes of membrane structures, and the antioxidant system in the development of surgical sepsis in dogs. Materials and Methods: Dogs with a provisional exclusion of sepsis were included in the study. The control group (Group 1) included clinically healthy dogs (n=5), and medium-breed dogs with systemic inflammation response syndrome (n=30) were categorized in the experimental group (Group 2). Along with hemogram and biochemical analysis, we determined the amount of malondialdehyde, glutathione, superoxide dismutase, catalase, glutathione reductase, and glucose-6-phosphate dehydrogenase on the 1st and 14th day of the study. Treatment included a thorough reorganization of the septic focus, followed by antibacterial therapy. Sick animals were injected with a drug (dexamethasone) that suppresses the synthesis and inhibits the action of inflammatory mediators. Decompensation of the functions of organs and systems was carried out using symptomatic therapy. Results: We found that enhanced lipid peroxidation of unsaturated fatty acids of membrane structures stimulates the generalization of inflammatory process, as evidenced by the significant deviation from the physiologically normal values of lipid peroxidation, C-reactive protein, blood cell count, etc. The course of systemic inflammation associated with surgical sepsis in animals can be attributed to several consistently developing processes that function as a result of increased purine mononucleotide catabolism, peroxide compound formation, and their excessive breakdown in reactions associated with the consumption of glutathione due to the insufficient recovery of glutathione disulfide. Conclusion: The amount of uric acid, glycosaminoglycans, hyaluronic acid in blood plasma, and the content of malondialdehyde, glutathione, and glutathione reductase in erythrocytes should be considered when assessing the severity of the systemic inflammatory process. The increased glutathione requirement in dogs with surgical sepsis requires intervention with pharmacological agents, and further research is needed in this aspect.

Immunomodulatory and Antioxidant Action of Selective Inhibitor of Arginase IInor-NOHA and Antihypoxants in Some Extreme Conditions

2015 ◽  
Vol 22 (3) ◽  
pp. 161-164
Author(s):  
Лосенок ◽  
P. Losenok ◽  
Наседкин ◽  
D. Nasedkin ◽  
Квачахия ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Selective Inhibitor ◽  
Control Group ◽  
Extreme Conditions ◽  
Secondary Products ◽  
Diene Conjugates ◽  
Pharmacological Agents ◽  
Combined Application ◽  
Arginase Ii

The study of the effects of a selective inhibitor of arginase II nor-NOHA and its combinations with Hy-poxenum, Kudesan, Cytoflavin on the functional activity of neutrophils, lipid peroxidation and content of stable metabolites of nitrogen in the blood serum of rats under the influence of some extreme conditions was carried out. It was found that under conditions of hypoxia that occurs in some extreme situations, there is a reduction of spontaneous and induced phagocytic and metabolic activity of neutrophils. Combined application of nor-NOHA, hypoxen and cytoflavin increases spontaneous PMA of neutrophils to values of the control group, but does not influence the values of NST Ind. The authors found that at hypoxia, there is an increase in the content of primary and secondary products of lipid peroxidation. Administration nor-NOHA and antihypoxants reduces the concentration of malondialdehyde at all combinations of drugs, and reduction of diene conjugates only in animals treated with nor-NOHA, hypoxen and cytoflavin. Total antioxidant activity increased as well, for all combinations of pharmacological agents, and catalase activity was increased combinations nor-NOHA + cytoflavin and nor-NOHA + hypoxen + cytoflavin. At hypoxia, there is a significant increase of stable NO metabolites in blood serum. Introduction nor-NOHA further increases their concentration, and at different combinations antigipoksantov administered in combination with an inhibitor of arginase II nor-NOHA marked reduction of NO metabolite to an animal in a state of hypoxia.

scholarly journals Evaluation of lipid peroxidation processes in patients with chronic parenteral viral hepatitis and HIV co-infection depending on degree of inflammatory process activity in the liver

2018 ◽  
Vol 90 (11) ◽  
pp. 37-43 ◽  
Author(s):  
L I Kolesnikova ◽  
M A Darenskaya ◽  
S I Kolesnikov ◽  
L A Grebenkina ◽  
M A Rashidova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Hiv Infection ◽  
Viral Hepatitis ◽  
Inflammatory Process ◽  
Chronic Viral Hepatitis ◽  
Reproductive Age ◽  
Inflammatory Activity ◽  
Control Group ◽  
Diene Conjugates ◽  
Peroxidation Processes

The aim of the study. To evaluate of parameters of lipid peroxidation - antioxidant defense changes in women of fertile age with chronic viral hepatitis (CVH) and with HIV (human immunodeficiency virus) infection, depending on the degree of inflammatory process activity in the liver. Materials and methods. 99 women of reproductive age were examined, of them 44 patients with chronic parenteral viral hepatitis, 27 patients with HIV co-infection (HIV + hepatitis B and/or C) and 28 practically healthy women (control group). Results. Patients with CVH in combination with HIV-infection with the presence of minimal and low degree of inflammatory activity in comparison with patients with CVH-monoinfection had a higher content of lipid peroxidation products (an increase in the content of diene conjugates and ketodienes and conjugated trienes), reduced values of total antioxidant activity, superoxide dismutase activity, as well as the concentration of fat-soluble vitamins α-tocopherol and retinol. In the group with CVH and HIV with moderate and high degree of activity, similar, but even more pronounced changes in the system of lipid peroxidation were observed. Conclusion. The presence of co-infection - CVH in combination with HIV infection with minimal and low, as well as moderate and high inflammatory activity, is characterized by more intensive lipid peroxidation processes and a pronounced lack of antioxidant factors than with CVH-monoinfection.

scholarly journals Atrazine induced toxic impacts on the survival, behavior, and biochemical aspects of Silver carp (Hypophthalmichthys molitrix)

2021 ◽  
Author(s):  
Naireen Nayab ◽  
Syed Ata Ur Rahman Shah ◽  
Sana Ullah ◽  
Muhammad Rauf ◽  
Shandana Ali ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Glutathione Reductase ◽  
Muscle Tissue ◽  
Total Protein ◽  
Silver Carp ◽  
Harmful Effect ◽  
Time Dependent ◽  
Control Group ◽  
Dependent Manner ◽  
Hypophthalmichthys Molitrix

Abstract The current research work was conducted to evaluate the acute toxicity of the widely used herbicide, Atrazine on the behavioral responses and biochemical changes in different tissues of Silver carp (Hypophthalmichthys molitrix). Adult fish were exposed to different concentrations of Atrazine for different time periods. Mortality of fish was observed with increase in the concentration of Atrazine and time of exposure. The LC50 of Atrazine for 96 hours was calculated as 13.7µl/L. Fish exposed to Atrazine showed fast swimming, loss of equilibrium and balance, gulping, pale body coloration, and an excess amount of mucous secretion covered the buccal cavity and gills. Total protein contents, antioxidant enzymes including catalase (CAT), peroxidase (POD), and glutathione reductase (GR) and lipid peroxidation (LPO), were measured in the brain, gills, liver, and muscle tissues for biochemical analysis. Atrazine exposure resulted in a significant time-dependent decrease in total protein content in the liver, gills, brain, and muscle tissue of fish compared to the control group. A slight increase was observed in the activities of CAT and POD in the control group, while a significant decrease was observed in the ATZ-treated groups from 24 to 96 h. The glutathione reductase activity was significantly increased in the ATZ treated groups compared to control group. Likely, LPO level also increased in the liver, gills, brain, and muscle tissue in a time-dependent and concentration-dependent manner in ATZ treated groups compared to control group. Concludingly, our results revealed that Atrazine is toxic to fish, alter enzymes and total protein level, and lipid peroxidation level in fish. This harmful effect of Atrazine maybe generalized into environment and reduce the Silver carp population in the natural freshwater bodies.

scholarly journals Effects of Repeatedly Heated Palm Oil on Serum Lipid Profile, Lipid Peroxidation and Homocysteine Levels in a Post-Menopausal Rat Model

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Siti Khadijah Adam ◽  
Ima Nirwana Soelaiman ◽  
Nor Aini Umar ◽  
Norhayati Mokhtar ◽  
Norazlina Mohamed ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Profile ◽  
Palm Oil ◽  
Serum Lipid ◽  
Unsaturated Fatty Acids ◽  
Thiobarbituric Acid ◽  
Serum Lipid Profile ◽  
Control Group ◽  
Cholesterol Diet ◽  
Sprague Dawley

Oxidized unsaturated fatty acids may contribute to the pathogenesis of atherosclerosis. In the present study, we examined the effects of heated palm oil mixed with 2% cholesterol diet on serum lipid profile, homocysteine and thiobarbituric acid reactive substances (TBARS) levels in estrogen-deficient rats. Twenty-four female Sprague Dawley rats were ovariectomized and then were divided equally into four groups. The control group was given 2% cholesterol diet only throughout the study period. The three treatment groups received 2% cholesterol diet fortified with fresh, once-heated or five-times-heated palm oil,respectively. Serum TBARS, lipid profile and homocysteine levels were measured prior to ovariectomy and at the end of four months of the study. Five-times-heated palm oil caused a significant increase in TBARS and total cholesterol (TC) compared to control (F = 22.529, p < 0.05). There was a significant increase in serum homocysteine in the control as well as five-times heated palm oil group compared to fresh and once-heated palm oil groups (F = 4.432, p < 0.05). The findings suggest that repeatedly heated palm oil increase lipid peroxidation and TC. Ovariectomy increases the development of atherosclerosis as seen in this study. Feeding with fresh and once-heated palm oil does not cause any deleterious effect but repeatedly heated oil may be harmful because it causes oxidative damage thereby predisposing to atherosclerosis.

RELATION OF THE MARKERS OF MYOCARDIAL DYSFUNCTION AND SYSTEMIC INFLAMMATION WITH STRUCTURAL AND FUNCTIONAL PARAMETERS OF THE LUNGS IN MYOCARDIAL INFARCTION PATIENTS

2018 ◽  
Vol 17 (2) ◽  
pp. 24-28
Author(s):  
O. M. Polikutina ◽  
Y. S. Slepynina ◽  
E. D. Bazdyrev ◽  
V. N. Karetnikova ◽  
O. L. Barbarach
Keyword(s):  
Myocardial Infarction ◽  
Systemic Inflammation ◽  
Myocardial Dysfunction ◽  
Control Group ◽  
Chronic Obstructive ◽  
Lung Pathology ◽  
Functional Changes ◽  
Functional Parameters ◽  
Lung Capacity ◽  
Copd Patients

Aim. To evaluate the structural and functional changes in the lungs of ST elevation myocardial infarction (STEMI) patients with absence or presence of chronic obstructive lung disease (COPD), and the relation with myocardial dysfunction and systemic inflammation.Material and methods. Totally, 189 STEMI patients included: group 1 — STEMI with COPD of moderate and mild grade, 2 — STEMI with no lung pathology. Groups were comparable by clinical and anamnestic parameters. Assessment of lung function and blood collection were done at 10­12 day of STEMI. For comparison of the parameters representing structural and functional changes in the lungs and comparison of C­reactive protein (CRP), N­terminal pro­brain natriuretic peptide (NT­proBNP) concentration, a control group was formed with no pulmonary pathology, comparable by age and sex with the STEMI patients.Results. In COPD patients, higher values revealed of the parameters representing the part of residual volumes in pulmonary structure. Higher residual volume (RV) was found also in STEMI and no COPD comparing to controls, however the relation RV/TLC (total lung capacity) was not higher than normal range. In both groups there were lower values of diffusion lung capacity (DLCO) comparing to controls. The lowest DLCO found in COPD patients. Concentration of NT­proBNP (H=41,6; p<0,001) and CRP (H=38,6; p<0,001) in COPD was significantly higher in STEMI with no COPD patients than in controls. The negative correlations found for NT­proBNP and CRP with forced expiratory volume 1 sec, FEV/FVC1, DLCO, and positive — with the values of thoracic volume, RV/TLC.Conclusion. In STEMI patients the increase revealed of residual lung volumes. Mostly the level of residual volumes is high in STEMI and COPD patients. There are associations of NT­proBNP and CRP with structural and functional parameters of the lungs regardless of COPD.

Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

2020 ◽  
Vol 28 (3) ◽  
pp. 323-333
Author(s):  
Sergej V. Ivanov ◽  
Ilya S. Ivanov ◽  
Evgenij G. Obyedkov ◽  
Liliya P. Popova
Keyword(s):  
Abdominal Wall ◽  
Inflammatory Reaction ◽  
Inflammatory Process ◽  
Anterior Abdominal Wall ◽  
Dynamic Change ◽  
Main Group ◽  
Control Group ◽  
Surgical Department ◽  
Regeneration Stage

Aim. To study the influence of deproteinized dialysate from blood of milk-fed calves on the type of exudative discharge and dynamics of inflammatory reaction after hernia repair with plastics of the anterior abdominal wall with hernioendoprosthesis of polypropylene. Materials and Methods. The study involved 59 patients being on stationary treatment in the surgical department of Kursk Regional Clinical Hospital. Patients were hospitalized for the herniation of small or medium dimension. The patients were divided to two groups: the main (n=30) and control (n=29) groups. After endoprosthetics, the patients of the control group were given complex conservative treatment. The patients of the main group, besides standard treatment, were administered deproteinized dialysate from blood of milk-fed calves intravenously in drips 10 ml + 200 ml of 0.9% sodium chloride solution within 7 days. For cytological examination and determination of the type of cytograms, the traumatic discharge was collected and analyzed using the method of M.F. Kamaev and M.A. Palthsev. Results. Cytomorphometric examination was conducted on the third, fifth and seventh day after endoprosthetics with the aim of studying dynamic changes. Determination of cell composition and also of its changes characteristic of each studied period, was necessary for obtaining further information characterizing inflammatory process in the region of placement of the endoprosthesis. After endoprosthetics in patients who were administered hemodialysate, the inflammatory reaction was less pronounced than in patients who did not receive the preparation. This was associated with a more dynamic change of stages of the inflammatory process. In patients of the main group who received deproteinized dialysate, regenerative type of inflammation first appeared on the fifth day and made 6.9%; by the seventh day the share of patients with the regenerative type rose to 17.5%, while in the patients of the control group no regeneration stage was observed in both periods. Conclusion. Analysis of the efficiency of influence of hemodialysate on the inflammatory reaction in plastics of the anterior abdominal wall with polypropylene endoprosthesis evidences faster course of all stages of inflammation and reduction of its intensity by 10%.

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

scholarly journals Evaluation of Neutrophil-to-Lymphocyte Ratio, Platelet-to-Lymphocyte Ratio, and C-Reactive Protein in Tension-Type Headache Patients

2021 ◽  
Author(s):  
Hasan Hüseyin Özdemir ◽  
Ahmet Dönder
Keyword(s):  
Systemic Inflammation ◽  
Tension Type Headache ◽  
Neutrophil To Lymphocyte Ratio ◽  
Control Group ◽  
C Reactive Protein ◽  
Platelet To Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Reactive Protein ◽  
Significant Difference ◽  
Type Headache

Abstract Objectives A tension headache is the most common type of headache, and its causes are multifactorial. A relationship has been shown between migraine headaches and neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and C-reactive protein (CRP). In this study, we investigated the NLR, PLR, and serum CRP levels in frequent episodic tension-type headache (FETTH) and chronic tension-type headache (CTTH) patients. Materials and Methods This retrospective study included 64 patients with FETTH, 80 patients with CTTH, and 60 healthy controls who were followed up in the neurology clinic. Hematological parameters were compared between the patient and control groups. Results In CTTH patients, platelets, NLR, PLR, and CRP values were statistically higher than in FETTH patients and patients in the control group. In FETTH patients, the PLR value was higher than in patients in the control group, but there was no statistically significant difference in NLR and CRP values between FETTH patients and patients in the control group. Also, there was no correlation between these values and age and gender. Conclusion Increase platelet count might have an effect on tension-type headache pathophysiology. Systemic inflammation parameters were shown to be significantly higher in CTTH patients. More comprehensive studies are needed to evaluate the effect of systemic inflammation on the chronicity of tension headaches.

scholarly journals Antioxidant Synergy of Mitochondrial Phospholipase PNPLA8/iPLA2γ with Fatty Acid–Conducting SLC25 Gene Family Transporters

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 678
Author(s):  
Martin Jabůrek ◽  
Pavla Průchová ◽  
Blanka Holendová ◽  
Alexander Galkin ◽  
Petr Ježek
Keyword(s):  
Lipid Peroxidation ◽  
Unsaturated Fatty Acids ◽  
Second Messengers ◽  
Redox Status ◽  
Mitochondrial Uncoupling ◽  
Mitochondrial Carrier ◽  
Mitochondrial Superoxide ◽  
Redox Activation ◽  
Mitochondrial Carrier Family ◽  
Alternatively Activated

Patatin-like phospholipase domain-containing protein PNPLA8, also termed Ca2+-independent phospholipase A2γ (iPLA2γ), is addressed to the mitochondrial matrix (or peroxisomes), where it may manifest its unique activity to cleave phospholipid side-chains from both sn-1 and sn-2 positions, consequently releasing either saturated or unsaturated fatty acids (FAs), including oxidized FAs. Moreover, iPLA2γ is directly stimulated by H2O2 and, hence, is activated by redox signaling or oxidative stress. This redox activation permits the antioxidant synergy with mitochondrial uncoupling proteins (UCPs) or other SLC25 mitochondrial carrier family members by FA-mediated protonophoretic activity, termed mild uncoupling, that leads to diminishing of mitochondrial superoxide formation. This mechanism allows for the maintenance of the steady-state redox status of the cell. Besides the antioxidant role, we review the relations of iPLA2γ to lipid peroxidation since iPLA2γ is alternatively activated by cardiolipin hydroperoxides and hypothetically by structural alterations of lipid bilayer due to lipid peroxidation. Other iPLA2γ roles include the remodeling of mitochondrial (or peroxisomal) membranes and the generation of specific lipid second messengers. Thus, for example, during FA β-oxidation in pancreatic β-cells, H2O2-activated iPLA2γ supplies the GPR40 metabotropic FA receptor to amplify FA-stimulated insulin secretion. Cytoprotective roles of iPLA2γ in the heart and brain are also discussed.

scholarly journals Interaction of Lactoferrin with Unsaturated Fatty Acids: In Vitro and In Vivo Study of Human Lactoferrin/Oleic Acid Complex Cytotoxicity

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1602
Author(s):  
Anna Elizarova ◽  
Alexey Sokolov ◽  
Valeria Kostevich ◽  
Ekaterina Kisseleva ◽  
Evgeny Zelenskiy ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Structural Changes ◽  
Unsaturated Fatty Acids ◽  
Structural Features ◽  
Control Group ◽  
Acid Complex ◽  
Hepatoma 22A ◽  
Constitutive Parameters

As shown recently, oleic acid (OA) in complex with lactoferrin (LF) causes the death of cancer cells, but no mechanism(s) of that toxicity have been disclosed. In this study, constitutive parameters of the antitumor effect of LF/OA complex were explored. Complex LF/OA was prepared by titrating recombinant human LF with OA. Spectral analysis was used to assess possible structural changes of LF within its complex with OA. Structural features of apo-LF did not change within the complex LF:OA = 1:8, which was toxic for hepatoma 22a cells. Cytotoxicity of the complex LF:OA = 1:8 was tested in cultured hepatoma 22a cells and in fresh erythrocytes. Its anticancer activity was tested in mice carrying hepatoma 22a. In mice injected daily with LF-8OA, the same tumor grew significantly slower. In 20% of animals, the tumors completely resolved. LF alone was less efficient, i.e., the tumor growth index was 0.14 for LF-8OA and 0.63 for LF as compared with 1.0 in the control animals. The results of testing from 48 days after the tumor inoculation showed that the survival rate among LF-8OA-treated animals was 70%, contrary to 0% rate in the control group and among the LF-treated mice. Our data allow us to regard the complex of LF and OA as a promising tool for cancer treatment.

Sign in / Sign up

Export Citation Format

Share Document