scholarly journals Chemical composition, antioxidant activity, and diuretic effect of Moroccan fresh bee pollen in rats

2020 ◽  
Vol 13 (7) ◽  
pp. 1251-1261 ◽  
Author(s):  
Asmae El Ghouizi ◽  
Nawal El Menyiy ◽  
Soraia I. Falcão ◽  
Miguel Vilas-Boas ◽  
Badiaa Lyoussi
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Chemical Composition ◽  
Urine Output ◽  
Antioxidant Properties ◽  
Dose Effect ◽  
Control Group ◽  
Pollen Extract ◽  
Diuretic Effect ◽  
Bee Pollen

Aim: This study investigated the chemical composition, antioxidant activity, and diuretic effect of Moroccan aqueous extract of fresh bee pollen (AEFBP) in normal rats. Materials and Methods: The chemical composition of the extracted bioactive compounds was assessed using liquid chromatography with diode array detection coupled to electrospray ionization (ESI) tandem mass spectrometry (LC/DAD/ ESI-MSn). 2,2-diphenyl-1-picrylhydrazyl and the reducing power were used to assess the antioxidant properties of the extract, together with the determination of total phenols and flavonoids. To assess the diuretic effect, 20 normal rats were divided into five groups: The first was a control group administered by distilled water (10 mL/kg body weight), the second group received furosemide (10 mg/kg body weight), the third group received 100 mg/kg body weight of AEFBP, the fourth group received 250 mg/kg body weight of AEFBP, and the fifth group received 500 mg/kg body weight of AEFBP for 30 days. Toward the end of this experiment, urine output was measured, and plasma and urine were sampled to analyze creatinine, potassium, chloride, and sodium levels. Results: N1,N5,N10-tri-p-coumaroylspermidine is a spermidine derivative and was the main compound in this sample, in a total of 19 compounds identified, including flavonoids, glucoside flavonoids, and methylated derivatives. Force feeding with the AEFBP induced a significant increase in urine output and urinary electrolyte levels with a dependent dose-effect without changes in plasma electrolytes, whereas furosemide decreased plasma potassium. Conclusion: Moroccan fresh bee pollen extract contains flavonols and spermidines that induce a potential antioxidant activity related to significant diuretic effect without changes in plasma composition.

Effect of Opuntia dejecta (Salm-Dyck) flowers in liver of fructose-fed rats: Biochemicals, enzymatic and histological studies

2021 ◽  
pp. 096032712110134
Author(s):  
O Zouaoui ◽  
K Adouni ◽  
A Jelled ◽  
A Thouri ◽  
A Ben Chrifa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Diabetes Type 2 ◽  
Male Rats ◽  
Control Group ◽  
Standard Drug ◽  
High Fructose ◽  
Group A ◽  
Histological Architecture

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

scholarly journals RELATIONSHIP BETWEEN ANTIOXIDANT PROPERTIES AND NUTRITIONAL COMPOSITION OF SOME GALACTOPOIETICS HERBS USED IN INDONESIA: A COMPARATIVE STUDY

2016 ◽  
Vol 8 (12) ◽  
pp. 236 ◽  
Author(s):  
Ade Chandra Iwansyah ◽  
Rizal M. Damanik ◽  
Lilik Kustiyah ◽  
Muhammad Hanafi
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Nutritional Composition ◽  
Total Flavonoids ◽  
Wide Range ◽  
Phenolics Content

<p><strong>Objective: </strong>The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.</p><p><strong>Methods: </strong>A wide range of analytical parameters was studied, including yield; pH; water activity (a<sub>w</sub>); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.</p><p><strong>Results: </strong>All of the galactagogue herbs, <em>viz</em>. <em>Plectranthus amboinicus </em>(Lour.) (PA), <em>Sauropus androgynus</em> (SA), <em>Moringa oleifera</em> (MO), and <em>Carica papaya</em> leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC<sub>50</sub>), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO&gt;CA&gt;PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.<strong></strong></p><p><strong>Conclusion: </strong>This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.</p>

scholarly journals Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract

2018 ◽  
Vol 51 (4) ◽  
pp. 164
Author(s):  
K. Khairiah ◽  
Irham Taufiqurrahman ◽  
Deby Kania Tri Putri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Antioxidant Properties ◽  
Linear Connection ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Control Group

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.

Chemical composition and physical properties of coffee (Coffea robusta) bee pollen in Daklak province, Vietnam

2021 ◽  
Author(s):  
L.P.T. Quoc
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Physical Properties ◽  
Nutritional Value ◽  
Dry Weight ◽  
Protective Capacity ◽  
Bee Pollen ◽  
Pellet Morphology ◽  
Colour Parameters ◽  
Coffea Robusta

AbstractThe main purpose of this study was to determine the chemical composition of monofloral bee-collected pollen from the coffee (Coffea robusta) plant and its pellet morphology. According to the results coffee bee pollen (CBP) has a high nutritional value. It contains a large percentage of protein, reducing sugar, and lipid. Also, vitamins (B2 and E) and minerals (K, Ca, and P) were found in CBP. In addition, the results revealed that the total polyphenols content (TPC) was 10.62 mg GAE/g dry weight (DW) and antioxidant activity (AA) of CBP was 5.52 μmol TE/g DW. Some physical properties of CBP pellet were recorded such as moisture, relative diameter, sphericity, bulk density, and colour parameters. The pollen pellet exhibits shapes of colour from light to dark yellow. These results pointed out that CBP can be used as a remarkable source of compounds with antioxidant activity and health-protective capacity for humans.

scholarly journals Performance indicators of broilers fed propolis and bee pollen additive

Poljoprivreda ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Ivana Prakatur ◽  
Matija Domaćinović ◽  
Božica Lachner ◽  
Zvonimir Steiner ◽  
Dalida Galović ◽  
...  
Keyword(s):  
Experimental Study ◽  
Body Weight ◽  
Weight Gain ◽  
Performance Indicators ◽  
Control Group ◽  
Significant Positive Effect ◽  
Feed Mixture ◽  
Bee Pollen ◽  
Positive Effect ◽  
Diet Supplementation

The aim of this study was to determine the effect of diet supplementation with propolis and/or bee pollen on the performance indicators of broilers. This experimental study was conducted on 200 Ross 308 broilers equally distributed by sex and divided into five groups. Throughout the whole study the control group of chickens was fed feed mixture. Feed mixture fed to the experimental groups of chickens contained additives (propolis and/or bee pollen, each supplement separately or in combination in a certain proportion). The average values of broilers body weight were significantly higher on 7th (p=0.001), 14th, 21st, 28th, 35th (p&lt;0.001) and 42nd (p=0.002) day of feeding in the experimental groups of broilers compared to the control group. The average values of broilers weight gain were significantly higher on 1st (p&lt;0.001), 2nd (p=0.002), 3rd (p&lt;0.001), 4th (p=0.029) and 5th (p=0.009) week of feeding in the experimental groups of broilers compared to the control group. This study has undoubtedly shown that propolis and bee pollen have significant positive effect on performance indicators of broilers.

scholarly journals In VivoAntioxidant Activity of Deacetylasperulosidic Acid in Noni

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
De-Lu Ma ◽  
Mai Chen ◽  
Chen X. Su ◽  
Brett J. West
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Superoxide Dismutase Activity ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dose Dependent

Deacetylasperulosidic acid (DAA) is a major phytochemical constituent ofMorinda citrifolia(noni) fruit. Noni juice has demonstrated antioxidant activityin vivoand in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group), 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activityin vivobut additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

scholarly journals Antioxidant Activity and Chemical Composition of Extracts from Fruiting Bodies of Xylotrophic Fungi Growing on Birch

2001 ◽  
Vol 14 (3) ◽  
pp. 339-353
Author(s):  
Alexander A. Ermoshin ◽  
◽  
Irina S. Kiseleva ◽  
Irina V. Nikkonen ◽  
Dominique S. Nsengiyumva ◽  
...  
Keyword(s):  
Amino Acids ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Properties ◽  
Biologically Active ◽  
Future Research ◽  
No Production ◽  
Fruiting Bodies ◽  
Fomitopsis Pinicola ◽  
Xylotrophic Basidiomycetes

The search for new natural sources of biologically active substances is a major issue in pharmaceutical industry. Xylotrophic basidiomycetes are common in forests worldwide, but as a prospective raw source of biologically active compounds they have not been studied as extensively as plants and other groups of fungi. The study is aimed to determine the chemical composition and antioxidant activity of extracts from 10 species of tinder fungi growing on birch and common in the forests in Russia. The chaga muchroom (Inonotus obliquus), traditionally used in medicine, was chosen as a standard species. Extracts from fruiting bodies were obtained with water or 95 % ethanol. They contained 4 to 8 types of free amino acids including 2 to 6 essential ones. Perennial basidiocarps were shown to be richer in phenolic compounds and poorer in amino acids than annual ones. Alkaloids and saponins were found in perennial basidiocarps of two species, saponins were also found in annual basidiocarps of one species. Water and alcohol extracts differed in composition and concentration of extractives, and showed different antioxidant (inhibition of lipid peroxidation) and antiradical (ABTS‑test, inhibition of NO production) activity. This way it was shown that the nature of the solvent extraction agent is important for the manifestation of biological activity. In most tests, water extracts from chaga showed better antioxidant properties; extracts from Piptoporus betulinus and Fomitopsis pinicola were also effective as antioxidants, which may be promising avenues for future research

scholarly journals Essential Oil Composition and Antioxidant Activity of Marrubium vulgare L. Growing Wild in Eastern Algeria

2013 ◽  
Vol 14 ◽  
pp. 17-24 ◽  
Author(s):  
Abderazak Abadi ◽  
Aicha Hassani
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Aerial Parts ◽  
Marrubium Vulgare ◽  
Eastern Algeria

In previous work [1], the essential oil of the aerial parts of Marrubium vulgare L. obtained by hydrodistillation was analysed by gas chromatography coupled to mass spectrometry (GC-MS) in order to determine their chemical composition. Fifty (50) components in the oil of M. vulgare were identified. The results demonstrated that the major components of the essential oil were: 4,8,12,16-Tetramethyl heptadecan-4-olid (16.97 %), Germacrene D-4-ol (9.61 %), α- pinéne (9.37 %), Phytol (4.87 %), Dehydro-sabina ketone (4.12 %), Piperitone (3.27 %), δ-Cadinene (3.13 %), 1-Octen-3-ol (2.35 %) and Benzaldehyde (2.31 %). In this study, the antioxidant properties of essential oil were examined. The results showed that this oil can be considered an effective source of antioxidants of natural origin. This is the first report on chemical composition of M. vulgare essential oil cultivated in Algeria and the original study on the antioxidant activity of M. vulgare essential oil. The antioxidant activity was investigated with one method: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method.

scholarly journals DIABETIC NEPHROPATHY

2018 ◽  
Vol 25 (07) ◽  
pp. 1117-1123
Author(s):  
Faiza Irshad ◽  
Rabia Sajjad Toor ◽  
Madiha Hussain
Keyword(s):  
Body Weight ◽  
Diabetic Nephropathy ◽  
Serum Creatinine ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Kidney Weight ◽  
Experimental Group

Background: Zingiber Officinale Roscoe (Zingiberaceae family) is knownas Ginger. It is famous for its antioxidant properties. Objectives: To evaluate the effects ofGinger aqueous extract on the serum creatinine and paired kidney weight in Alloxan induceddiabetic nephropathy of albino rats. Study Design: Experimental study. Period: 06 months01-01-2013 to 30 June 2013. Setting: Anatomy Department, Sheikh Zayed, PGMI Lahore.Materials and Methods: Diabetes mellitus was induced with Alloxan intraperitoneally (150 mg/kg body weight) in Experimental groups B & C. Then the rats of experimental group C received200mg/kg body weight of ginger aqueous extract by gavage daily for five weeks starting from8th day after Alloxan injection. Results: Serum creatinine levels increased more in experimentalgroup B as compared to experimental group C. Group wise comparison of creatinine levelrevealed that the difference among control (A group) and experimental (B & C Groups) wassignificant having p-value <0.001. We observed that Paired kidney weight in experimentalgroup B increased as compared to control group A. Less increase in the paired kidney weightwas observed in experimental group C as compared to experimental group B. The differenceof mean paired kidney weight among three groups was significant having p-value <0.001.Conclusion: The results of the present study indicated that the co-treatment of Ginger aqueousextract prevented alloxan induced diabetic nephropathy in albino rats. The aqueous extract ofGinger showed amazing results on paired kidney weight.

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