scholarly journals THE COMPARATIVE STUDY OF PHENOLIC COMPOUNDS IN TRIFOLIUM L. SPECIES IN SIBERIA

2017 ◽  
pp. 97-104
Author(s):  
Валерия (Valeriia) Юрьевна (Iur'evna) Андреева (Andreeva) ◽  
Галина (Galina) Ильинична (Il'inichna) Калинкина (Kalinkina) ◽  
Татьяна (Tat'iana) Викторовна (Viktorovna) Полуэктова (Poluektova) ◽  
Вера (Vera) Александровна (Aleksandrovna) Гуляева (Guliaeva)
Keyword(s):  
Phenolic Compounds ◽  
Comparative Study ◽  
Aerial Part ◽  
Trifolium Pratense ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
Material Base ◽  
Standard Samples ◽  
Trifolium Pratense L

Conducted a comparative study of the phenolic compounds content, as well as the qualitative and quantitative composition of flavonoids and isoflavonoids are 8 species of clover (Trifolium L.) flora of Siberia with the purpose of identifying the most promising species as sources of biologically active complexes.It is established that the amount of phenolic compounds in the aerial part of Trifolium pratense L, T.hybridum L., T. arvense L, T. repens L., T.montanum L., T. medium L., T. aurea (Pollich) Green, T. lupinaster L. is of 2,37–3,78%. The content of flavonoids in the samples changed from 1,91 to 3,78 %. By the method of chromatography on paper and thin-layer chromatography to be closest in composition of flavonoids (rutin, quercetin and cynaroside) to the T. pratense L are T.montanum L., T. medium L., T. lupinaster L. and T. aurea (Pollich) Green. The content of isoflavones in the aerial part of the clover species flora of Siberia ranged from 0,27 to 2,58%. The most promising are the T. medium L., T. repens L. and T.hybridum L.. Formononetin, biochanin, genistein, ononin were identified in the composition of these species by chromatographic comparison with standard samples. Trifolium pratense L, T.hybridum L., T. lupinaster L., T. medium L. with sufficient raw material base in the flora of Siberia can be recommended for further study as sources of flavonoids, including isoflavonoids.

scholarly journals Investigation of phenolic compounds of estrogenic wormwood (Artemisia dracunculus L.) according to plant introduction in Zhytomyr Polissya

2016 ◽  
Author(s):  
I. Ivashchenko
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antimicrobial Properties ◽  
Plant Introduction ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
Chromatography Method ◽  
Artemisia Dracunculus ◽  
Chromatography Analysis

High-performance liquid chromatography method has been used to evaluate qualitative and quantitative composition of certain phenolic compounds obtained from areal parts of Artemisia dracunculus L. introduced in Zhytomyr Polissya. 31 phenolic compounds have been detected, among which the following flavonoids were identified: rutin (1.30±0.04 mg/g), luteolin-7-glycoside (0.34±0.03), apigenin-7-glycoside (0.30±0.01) and isochlorogenic acid (0.16± 0.02 mg/g), where rutin was the dominant component. The total amount of phenolic compounds in air-dried raw material constituted 51.24±0.12 mg/g (5.12%). The employed chromatography analysis of phenolic compounds from Artemisia dracunculus areal parts shows that the plant may be considered as a valuable source of biologically active compounds of phenolic origin. Phenolic compounds are likely to determine the antimicrobial properties of the plant, established earlier. Further in-depth study and cultivation of Artemisia dracunculus in Zhytomyr Polissya have a great potential for pharmaceutical and food industries, cosmetics and development of therapeutic antioxidant diets.

scholarly journals Comparative study of the elemental composition in raw materials of plants of genus Geum L.

2021 ◽  
pp. 70-77
Author(s):  
С. А. Козира ◽  
Т. М. Гонтова ◽  
С. І. Степанова ◽  
В. П. Гапоненко ◽  
В. В. Машталер ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Comparative Study ◽  
Atomic Absorption ◽  
Elemental Composition ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Material Base ◽  
Plant Raw Materials ◽  
Microelement Composition

Among the relevant for the modern pharmacy problems, there is a need for the rational complex use of known medicinal plants, as well as the search for new sources of natural biologically active compounds in order to expand the list of official medicinal plants and raw material base. Species of genus Geum L. contain a number of biological active substances and are promising sources of raw materials for the production of antimicrobial, astringent, anti-inflammatory, and hemostatic medicines. Pharmacological activity of these plants results from their multi-component chemical composition, including tannins, flavonoids, macro- and microelements and other compounds. The aim of thе study was to compare the elemental composition of herb and rhizomes with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. of Ukrainian flora. The objects of the study were the samples of herb and rhizome with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. (dried raw materials) which were collected in the Kharkiv region, Ruski Tishki village, in June-August 2018–2019. The determination of the qualitative composition and quantitative content of elements was carried out by atomic absorption spectroscopy with atomization in an air-acetylene flame using a spectrometer CAS 120 («Selmi», Sumy, Ukraine). In the studied samples, 19 elements were determined, including 5 macroelements and 14 microelements. The data about accumulation of elements in investigated herb of G. urbanum L., G. aleppicum Jacq., G. rivale L. can be arranged in descending order as follows: K > Ca > Si > Mg > P > Fe > Al > Na > Sr > Zn, and in rhizomes with roots as follows – K > Si > Ca > Mg > Fe > P > Al > Na > Sr > Zn. The content of toxic elements such as cobalt, cadmium, arsenic, and mercury was within the permissible limits for plant raw materials and food. For the first time, a comparative study of the macro- and microelement composition in herb and rhizomes with roots of G. urbanum L., G. aleppicum Jacq. and G. rivale L. has been carried out. Using the method of atomic absorption spectral analysis, 19 elements have been identified and their content has been determined, among which К, Ca, Mg, Si, P, Fe, Al, Na, Mn, Zn predominantly accumulate.

scholarly journals Comparative study of phenolic compounds of the herb of Betonica L. genus species of flora of Ukraine

2021 ◽  
pp. 66-75
Author(s):  
Iryna Sas ◽  
Andrii Hrytsyk ◽  
Taras Koliadzhyn ◽  
Oleh Koshovyi
Keyword(s):  
Phenolic Compounds ◽  
Comparative Study ◽  
Hydroxycinnamic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
New Drugs ◽  
Raw Material ◽  
Wide Range ◽  
Nitrogen Containing Compounds ◽  
Active Substances

Species of Betonica L. genus are widespread in Ukraine and contain different groups of biologically active substances: hydroxycinnamic acids, flavonoids, tannins, iridoids, terpenoids, steroids, essential oil, organic acids, vitamin K, nitrogen-containing compounds, phenylethanoid glycosides. Species of Betonica L. genus show a wide range of pharmacological activity (anti-inflammatory, antioxidant, choleretic, diuretic, sedative, antitumor, antihypertensive, etc.) and phenolic compounds are one of the most important and promising groups of biologically active substances of these plants. The aim. The aim of the work was to conduct a comparative study of the phenolic compounds of the herb of Betonica L. genus species of flora of Ukraine. Materials and methods. The object of the study was the herb of Betonica peraucta and Betonica brachydonta harvested in the phase of mass flowering of the plant in Ivano-Frankivsk region. The study of phenolic compounds was carried out by paper chromatography, HPLC and spectrophotometry. Results. 7 components of tannins, 4 flavonoids, 5 hydroxycinnamic acids, 2 coumarins were identified and quantified by HPLC in the studied raw material. The quantitative content of the main groups of phenolic compounds in the herb of Betonica peraucta and Betonica brachydonta was determined by the method of absorption spectrophotometry: polyphenols – 5.96 % and 4.82 %, tannins – 1.62 % and 0.68 %, flavonoids – 2.07 % and 1.13 %, hydroxycinnamic acids – 7.01 % and 3.58 %, respectively. Conclusions. As a result of the conducted studies it was found that the content of phenolic compounds in the herb of Betonica peraucta is significantly higher than in the herb of Betonica brachydonta. Therefore, this species is promising for further research and creation of new drugs

scholarly journals Theoretical and experimental substantiation of a fungal therapeutic drug with immunomodulating and antitumor action according to the authors’ prescription

2018 ◽  
Vol 9 (1) ◽  
pp. 3-10
Author(s):  
V. A. Кuchmistov ◽  
N. O. Коziko ◽  
O. F. Кuchmistova ◽  
O. P. Shmatenko ◽  
V. O. Таrasenko ◽  
...  
Keyword(s):  
Lentinula Edodes ◽  
Pharmaceutical Preparation ◽  
Biologically Active ◽  
Raw Material ◽  
Antitumor Action ◽  
Therapeutic Drug ◽  
Steady Increase ◽  
Quantitative Composition ◽  
Material Base ◽  
Dry Powder

The article is devoted to a promising direction in pharmacological mycology and biotechnology, namely the use of Basidiomycetes as a biologically active substance for preparation of pharmaceuticals. The anticancer and immunostimulating properties of Basidiomycetes result from the structural characteristics of the polysaccharides: the presence of β-(1 → 3) connections in the main chain and an additional β-(1 → 6) branched part of glucans. Attention is focused on Shiitake mushrooms (Lentinula edodes) as a unique biosystem with a strong ability to be involved in the regulatory mechanism of human immunomodulation. The purpose of the theoretical and experimental study is development of a pharmaceutical preparation from Shiitake biomass (including its immune cells), the effectiveness of which is considered from the standpoint of the chemistry of natural compounds. Having a 3000-year history of cultivation and application, this mushroom is again of interest to scientists around the world because of investigation into its therapeutic and prophylactic potentials. For the first time, the biotechnological method of cultivating L. edodes biomass was developed with the use of deep cultivation, adapted to the plant raw material base of Ukraine. Malt wort was used as a nutrient medium without processing Humulus lupulus. The analysis of research and preclinical studies of specific activity of the designated biosystem showed a steady increase in human immune status, particularly the antitumor status. This gives special relevance to the evaluation of the properties of the designated biosystem in order to further develop the corresponding fungo-therapeutic immunomodulatory and antitumor drug. According to the results of technological research: physico-chemical characteristics of Shiitake biomass dry powder (including crystallographic) were studied; the possibility of its tableting using only antifriction auxiliaries was substantiated; the optimum technology of tablet manufacturing with an operating name "Shitavit" was presented, an integrated approach to its creation was presented, the qualitative and quantitative composition of the drug was experimentally developed. The direct compression method was rejected due to unsatisfactory performance of all samples of tablets. The chosen technology of using preliminary granulation and the possibility of short-term contact of the L. edodes dry powder with a granulation liquid and an adjustable temperature regime allows all the properties of the biologically active substances of the designated raw material to be preserved. The research results for all series of the designated object showed relative stability of parameters for all indicators in laboratory samples. The information presented in this article is a guide to further research, necessary for a better understanding of the healing properties of fungal polysaccharides, increasing the use of broad-spectrum fungi-based preparations,leading to improvement of the quality of human life.

Technological aspects of alcohol distillates production from non-traditional sugar-raw materials

2021 ◽  
Vol 9 (16) ◽  
pp. 90-97
Author(s):  
Kateryna Danilova ◽  
◽  
Oksana Zavarzina ◽  
Keyword(s):  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Material Base ◽  
Growth Substances ◽  
Technological Properties ◽  
Actual Problem ◽  
Cane Molasses ◽  
Yeast Biomass ◽  
Nitrogen Phosphorus

An actual problem of the alcohol industry development is the expansion of the raw material base through the use of new, cheaper types of carbohydrate sources. Recently, glucose-fructose syrup has been of increasing interest to producers of ethanol. Among the producers of craft drinks, in particular rum-based drinks, cane molasses is in increasing demand. The article presents the technological aspects of the fermentation process of nonconventional sugar-containing raw materials on the example of glucose-fructose syrup and cane molasses in ethanol by different yeast strains. It is determined that glucose-fructose syrup and cane molasses are raw materials depleted of nitrogen, phosphorus and growth substances necessary for the yeast activity. To improve the technological properties of raw materials in the molasses, nitrogen, phosphorus nutrition and corn extract as a growth substance shall be added to a mash. Glucose-fructose syrup is of pH 3.68 and of acidity 0.12, which negatively affects the fermentation. It is established that in order to create optimal living conditions for yeast, it is necessary to add substances to the glucose-fructose syrup that will contribute to the safety of development and prevent the pH 3.5. It was determined that the alcohol yield from 1 ton of glucose-fructose syrup is 31.0 dal, from 1 ton of cane molasses is 24.0 dal on the yeast 46ED and 22 dal on the TegaYeast without the use of growth substances and vitamins necessary for the reproduction of yeast biomass. Under conditions of improving the technological properties of the mash from the glucose-fructose syrup and cane molasses by adding biologically active substances promoting the synthesis of yeast biomass, the ethanol content in the fermented mashes increases up to 11.9 12% vol.

scholarly journals Berries and Leaves of Actinidia kolomikta (Rupr. & Maxim.) Maxim.: A Source of Phenolic Compounds

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Laima Česonienė ◽  
Paulina Štreimikytė ◽  
Mindaugas Liaudanskas ◽  
Vaidotas Žvikas ◽  
Pranas Viškelis ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Content ◽  
Ascorbic Acid Content ◽  
Free Radical Scavenging ◽  
Raw Material ◽  
Quantitative Composition ◽  
Pharmaceutical Industries

Berries of Actinidia kolomikta (A. kolomikta) are known for high ascorbic acid content, but the diversity of phenolic compounds has been little studied. The present research aimed to investigate phenolic compounds and antioxidant activity in berries and leaves of twelve A. kolomikta cultivars. The UHPLC-ESI-MS/MS technique was used to determine differences among cultivars in the quantitative composition of individual phenolic compounds. Antioxidant activity was determined by DPPH• free radical scavenging and CUPRAC methods. In the present study, 13 phenolic compounds were detected in berries, whereas leaves contained 17 phenolic compounds. Flavonols were the primary class found in both berries and leaves; other identified phenolic compounds were flavan-3-ols, flavones and, phenolic acids; and dihydrochalcone phloridzin was identified in the leaves. The amount and variety of phenolic compounds in berries and leaves and antioxidant activity were found to be cultivar-dependent. The highest total content of phenolic compounds was found in the leaves of the cultivar ‘Aromatnaja’ and in the berries of the cultivar ‘VIR-2’. Results of this study have confirmed that berries and leaves of A. kolomikta could be a valuable raw material for both food and pharmaceutical industries.

scholarly journals Fast screening of some flavonoids content in raw plant materials: opportunities of 1H NMR spectroscopy

2020 ◽  
Vol 169 ◽  
pp. 02006
Author(s):  
Gennady Kalabin ◽  
Vasilii Vasil’ev ◽  
Vasilii Ivlev ◽  
Vasilii Babkin
Keyword(s):  
Nmr Spectroscopy ◽  
Plant Biomass ◽  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Standard Samples ◽  
Plant Materials ◽  
H Nmr ◽  
Fast Screening ◽  
Raw Plant Materials

Environmental monitoring and assessment of the prospects for extracting biologically active substances (BAS) from various types of plant biomass requires the development of simple and fast methods for measuring their content in raw materials. A new approach for measuring the content of various flavonoids groups in plant raw material using 1H NMR spectroscopy has been developed, which allows to characterize its resource capabilities and study the effects on their composition different environmental factors without complex sample preparation and standard samples.

scholarly journals Study of the antimicrobial and fungicidal activity of the essential oil Thymus x citriodorus (Pers.) Schreb. var. “Silver Queen”

2021 ◽  
Vol 14 (2) ◽  
pp. 211-214
Author(s):  
Ya. M. Steshenko ◽  
O. V. Mazulin ◽  
N. M. Polishchuk
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Fungicidal Activity ◽  
Wide Spectrum ◽  
Bactericidal Effect ◽  
Biologically Active ◽  
Raw Material ◽  
Diffusion Method ◽  
Material Base

The main problem of modern phytotherapy is the medicinal plants that have a sufficient raw material base and contain a large number of biologically active substances. Treatment of diseases with synthetic drugs leads to the development of resistance to pathogenic microflora and the appearance of allergic reactions. Therefore, to solve this problem, it is necessary to find new plant-based antimicrobials that are safe for long-term use and have a wide spectrum of action. The aim of the work was to study the antibacterial and fungicidal activity of the essential oil of Thymus x citriodorus (Pers.) Schreb. var. “Silver Queen”. Materials and methods. The essential oil of Thymus x citriodorus (Pers.) Schreb. var. “Silver Queen” was used for the experimental part. To test the antimicrobial and antifungal action of the essential oil, reference test strains, gram-positive and gram-negative bacteria, as well as yeast-like fungi of the genus Candida were selected. The studies were performed in vitro using the disco-diffusion method. Results. Studies have shown that the essential oil of Thymus x citriodorus (Pers.) Schreb. var. “Silver Queen” has a significant antibacterial effect against S. aureus ATCC 25923 (diameters of growth inhibition were 14.60 ± 1.52 mm) and fungicidal effect Candida albicans ATCC 885-6530 (29.30 ± 2.82 mm). Antibacterial activity of the essential oil was detected in relation to E. coli (19.60 ± 1.85 mm). It was experimentally proven that the essential oil had no bactericidal effect on the test strain of P. aeruginosa. Conclusions. It was found that the essential oil of the studied hybrid species Thymus x citriodorus (Pers.) Schreb. var. “Silver Queen” shows a significant antibacterial activity and is promising for further research.

DEVELOPMENT AND VALIDATION OF PROCEDURE FOR QUANTITATIVE DETERMINATION OF SUM OF FLAVONOIDS IN STOCK OF Salvia stepposa

2020 ◽  
pp. 52-57
Author(s):  
Polukhina T.S.
Keyword(s):  
Plant Species ◽  
Folk Medicine ◽  
Biologically Active ◽  
Raw Material ◽  
Cultivated Plants ◽  
Material Base ◽  
Upper Respiratory Tract ◽  
Limited Information ◽  
Treatment And Prevention ◽  
Relative Standard

On the territory of the Russian Federation, about 80 species grow, both wild-growing and cultivated, plants of the genus sage Salvia L. family Lamiaceae. In scientific and folk medicine, they are widely used as anti-inflammatory, antimicrobial, expectorant and antispasmodic agents for the treatment and prevention of the upper respiratory tract, dentistry. However, many plant species of the genus Salvia L. are not yet sufficiently studied and are not in demand for medical use. In this regard, the problem of finding new plant species of the genus Salvia L., expanding the raw material base and developing highly effective drugs based on them, is quite urgent. Of scientific interest is a Salvia stepposa L., which grows in the Astrakhan region. To date, there is limited information in the literature about the chemical composition and quantitative content of some biologically active substances (BAV) of this plant. As a result of the experiment, a spectrophotometric technique for quantifying the sum of flavonoids in terms of rutin in the leaves of steppe sage harvested in the Astrakhan region was developed. Validation of the proposed methodology on indicators: correctness, convergence, reproducibility, specificity, linearity. The error of the single determination with 95% probability did not exceed ± 3.25%, the relative standard deviation did not exceed 3%. The content of the sum of flavonoids in terms of rutin ranged from 5.72 to 5.82%. Thus, the developed methodology can be recommended for inclusion in the FS project «Salvia stepposa L. leaves».

TECHNOLOGY OF PRODUCTION OF BIOLOGICALLY ACTIVE PLANT COMPLEX: DETERMINATION OF REGULATED PARAMETERS, FUNCTIONAL PROPERTIES

2019 ◽  
Keyword(s):  
Functional Properties ◽  
Viral Infections ◽  
Raw Materials ◽  
Extraction Process ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
Comprehensive Treatment ◽  
Plant Raw Materials ◽  
Technology Of Production

The technology of production of a biologically active phytocomplex in the form of a biologically active additive has been discussed. The qualitative and quantitative composition of the formulation, which forms the functional properties of a specialized product, has scientifically been determined. The main stages of production include the preparation and input control of the raw material, the preparation of a mixture for encapsulation, including the steps of dispensing, sieving, grinding and mixing at a rate of 100 kg / 1 h, encapsulation and deducting, packaging, packaging and storage. A description of the technology of ex-traction of plant raw materials has been given, which is of great importance in the formation of consumer properties of the product being developed. The extraction process includes: obtaining liquid recovery us-ing demineralized water with dissolved in it sodium bicarbonate in an amount of 8% of the dry feed con-tent. The extractant-raw ratio is 1:13, the extraction time is 4 hours for each time; thickening of the liquid extract. It is carried out by means of vacuum in a vacuum evaporator plant before obtaining an extract with a solids content of not less than 20%; obtaining a dry extract. It is carried out by spray drying at a temperature of 90-95 ° C. The advantage of the developed technology of dietary supplements is the possi-bility of combining several differently directed biologically active substances in one capsule. The gelati-nous membrane reliably protects the contents from various environmental factors. Functional properties of the formulation formula of a specialized product are aimed at the prevention and comprehensive treatment of infectious viral diseases, confirmed by the conclusion of experts from the Russian Federal Service for Consumer Rights and Consumer Protection and the results of clinical trials in a group of pa-tients with acute respiratory viral infections. The developed product has been approved and produced at the enterprises of the company «Art Life».

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