scholarly journals Investigation of phenolic compounds of estrogenic wormwood (Artemisia dracunculus L.) according to plant introduction in Zhytomyr Polissya

2016 ◽  
Author(s):  
I. Ivashchenko
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antimicrobial Properties ◽  
Plant Introduction ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
Chromatography Method ◽  
Artemisia Dracunculus ◽  
Chromatography Analysis

High-performance liquid chromatography method has been used to evaluate qualitative and quantitative composition of certain phenolic compounds obtained from areal parts of Artemisia dracunculus L. introduced in Zhytomyr Polissya. 31 phenolic compounds have been detected, among which the following flavonoids were identified: rutin (1.30±0.04 mg/g), luteolin-7-glycoside (0.34±0.03), apigenin-7-glycoside (0.30±0.01) and isochlorogenic acid (0.16± 0.02 mg/g), where rutin was the dominant component. The total amount of phenolic compounds in air-dried raw material constituted 51.24±0.12 mg/g (5.12%). The employed chromatography analysis of phenolic compounds from Artemisia dracunculus areal parts shows that the plant may be considered as a valuable source of biologically active compounds of phenolic origin. Phenolic compounds are likely to determine the antimicrobial properties of the plant, established earlier. Further in-depth study and cultivation of Artemisia dracunculus in Zhytomyr Polissya have a great potential for pharmaceutical and food industries, cosmetics and development of therapeutic antioxidant diets.

scholarly journals THE COMPARATIVE STUDY OF PHENOLIC COMPOUNDS IN TRIFOLIUM L. SPECIES IN SIBERIA

2017 ◽  
pp. 97-104
Author(s):  
Валерия (Valeriia) Юрьевна (Iur'evna) Андреева (Andreeva) ◽  
Галина (Galina) Ильинична (Il'inichna) Калинкина (Kalinkina) ◽  
Татьяна (Tat'iana) Викторовна (Viktorovna) Полуэктова (Poluektova) ◽  
Вера (Vera) Александровна (Aleksandrovna) Гуляева (Guliaeva)
Keyword(s):  
Phenolic Compounds ◽  
Comparative Study ◽  
Aerial Part ◽  
Trifolium Pratense ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
Material Base ◽  
Standard Samples ◽  
Trifolium Pratense L

Conducted a comparative study of the phenolic compounds content, as well as the qualitative and quantitative composition of flavonoids and isoflavonoids are 8 species of clover (Trifolium L.) flora of Siberia with the purpose of identifying the most promising species as sources of biologically active complexes.It is established that the amount of phenolic compounds in the aerial part of Trifolium pratense L, T.hybridum L., T. arvense L, T. repens L., T.montanum L., T. medium L., T. aurea (Pollich) Green, T. lupinaster L. is of 2,37–3,78%. The content of flavonoids in the samples changed from 1,91 to 3,78 %. By the method of chromatography on paper and thin-layer chromatography to be closest in composition of flavonoids (rutin, quercetin and cynaroside) to the T. pratense L are T.montanum L., T. medium L., T. lupinaster L. and T. aurea (Pollich) Green. The content of isoflavones in the aerial part of the clover species flora of Siberia ranged from 0,27 to 2,58%. The most promising are the T. medium L., T. repens L. and T.hybridum L.. Formononetin, biochanin, genistein, ononin were identified in the composition of these species by chromatographic comparison with standard samples. Trifolium pratense L, T.hybridum L., T. lupinaster L., T. medium L. with sufficient raw material base in the flora of Siberia can be recommended for further study as sources of flavonoids, including isoflavonoids.

scholarly journals ФУНГІЦИДНІ ВЛАСТИВОСТІ ЕФІРООЛІЙНИХ РОСЛИН РОДУ ARTEMISIA ВІДНОСНО FUSARIUM OXYSPORUM

2015 ◽  
Vol 5 (02) ◽  
pp. 44
Author(s):  
I. V. Ivashchenko
Keyword(s):  
Phenolic Compounds ◽  
Fusarium Oxysporum ◽  
Fungicidal Activity ◽  
Antimicrobial Properties ◽  
Water Extract ◽  
Component Composition ◽  
Biologically Active ◽  
Aromatic Plants ◽  
Artemisia Dracunculus ◽  
Artemisia Abrotanum

<p>The article establishes the fungicidal activity of water extracts of <em>Artemisia</em><em> </em><em>maritim</em><em>а</em> L., <em>Artemisia</em><em> </em><em>austriaca Jacq.,</em> under the concentration of 100, 50 and 25 mg/ml on dry matter with regard to the phytopathogenic mushroom <em>Fusarium oxysporum</em>. It also shows the fungistatic influence of extract of Artemisia dracunculus L. under concentration 25 and 50 mg/ml, fungicidal – under 100 mg/ml. Concerning <em>Artemisia abrotanum</em> L., the slow growth of mushroom is observed under the concentration 25 mg/ml, fungicidal effect – under 50 and 100 mg/ml.</p> <p>The paper provides the information on the component composition of ethereal oil and phenolic compounds of <em>Artemisia</em><em> </em><em>maritim</em><em>а</em>, <em>Artemisia</em><em> </em><em>austriaca</em>,<em> Artemisia abrotanum</em><em>, </em><em>Artemisia dracunculus</em>, cultivated in Zhytomyr Polissya. The chief ingredients of ethereal oil which is synthesized by the plant of <em>Artemisia </em><em>abrotanum</em> are 1,8-cineole (30.44%) and camphor (31.92%). A high 1,8-cineole and camphor content determines antimicrobial properties of the plants.</p> <p>Amount of phenolic compounds in the air-dry raw <em>Artemisia </em><em>abrotanum</em> is 2.98 percent. By the method of highly efficient solution chromatography (HESChr) in the grass of <em>Artemisia abrotanum </em>we have detected 23 phenolic compounds, of which we identified such flavonoids as rutin, luteolin-7-glycoside as well as caffeic, chlorogenic and isochlorogenic acids.</p> <p>The main compounds of ethereal oil of <em>Artemisia austriaca</em> are trans-verbenole (30.77 %), pinocarvone (10.77 %) and sabinilacetate (18.16 %).<strong> </strong>In the grass of <em>Artemisia austriaca </em>we have detected 31 phenolic compounds, of which we identified such flavonoids as rutin, apigenin, quercetin-bioside and the following acids: caffeic, chlorogenic, and isochlorogenic. Amount of phenolic compounds in the air-dry raw Austrian wormwood is 27.25 mg / g (2.73 %).</p> <p>The main component of ethereal oil of <em>Artemisia dracunculus is </em>methyleugenol (94.65 %). We have discovered 31 phenolic compounds in the grass of linear-leaved wormwood, from which four substances such as rutin, luteolin-7-glycoside, apigenin-7-glycoside and isochlorogenic acid have been identified. The dominant compounds of ethereal oil of <em>Artemisia maritima</em> are α-thujone (41.59 %) and camphor (23.56 %).</p> <p>Obviously, the fungicidal properties of plants are conditioned by the quantitative and qualitative content of biologically active substances – ethereal oils, fenolic compounds and other matters. The paper draws attention to the prospects of the further more detailed study of extracts of<strong> </strong>aromatic plants of the genus <em>Artemisia</em> with the aim of producing antibacterial and antifungal herbal preparations.</p> <p><em>Key words<strong>:</strong></em><strong><em> </em></strong><em>Artemisia, Fusarium oxysporum, water extract, fungicidal activity, aromatic plants.</em></p> <p><em> </em></p>

scholarly journals Application of the thin-layer chromatography method for analysis of plant antioxidant activity

Food systems ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 26-30
Author(s):  
N. V. Kupaeva ◽  
E. R. Vasilevskaya ◽  
L. V. Fedulova ◽  
E. A. Kotenkova
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Chromatography Method ◽  
Physicochemical Methods ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Active Substances

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.

scholarly journals Berries and Leaves of Actinidia kolomikta (Rupr. & Maxim.) Maxim.: A Source of Phenolic Compounds

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Laima Česonienė ◽  
Paulina Štreimikytė ◽  
Mindaugas Liaudanskas ◽  
Vaidotas Žvikas ◽  
Pranas Viškelis ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Content ◽  
Ascorbic Acid Content ◽  
Free Radical Scavenging ◽  
Raw Material ◽  
Quantitative Composition ◽  
Pharmaceutical Industries

Berries of Actinidia kolomikta (A. kolomikta) are known for high ascorbic acid content, but the diversity of phenolic compounds has been little studied. The present research aimed to investigate phenolic compounds and antioxidant activity in berries and leaves of twelve A. kolomikta cultivars. The UHPLC-ESI-MS/MS technique was used to determine differences among cultivars in the quantitative composition of individual phenolic compounds. Antioxidant activity was determined by DPPH• free radical scavenging and CUPRAC methods. In the present study, 13 phenolic compounds were detected in berries, whereas leaves contained 17 phenolic compounds. Flavonols were the primary class found in both berries and leaves; other identified phenolic compounds were flavan-3-ols, flavones and, phenolic acids; and dihydrochalcone phloridzin was identified in the leaves. The amount and variety of phenolic compounds in berries and leaves and antioxidant activity were found to be cultivar-dependent. The highest total content of phenolic compounds was found in the leaves of the cultivar ‘Aromatnaja’ and in the berries of the cultivar ‘VIR-2’. Results of this study have confirmed that berries and leaves of A. kolomikta could be a valuable raw material for both food and pharmaceutical industries.

scholarly journals Determination of pesticide residues by chromatographic methods for food safety

2021 ◽  
Vol 12 (3) ◽  
pp. 111-118
Author(s):  
N. Yu. Tereshchenko ◽  
◽  
O. Yu. Kursenko ◽  
O. I. Khyzhan ◽  
O. I. Khyzhan ◽  
...  
Keyword(s):  
Plant Extract ◽  
High Performance ◽  
Solid Phase ◽  
Raw Materials ◽  
Apple Fruit ◽  
Raw Material ◽  
Benzimidazole Derivatives ◽  
Quantitative Composition ◽  
Variable Component ◽  
Selective Solvents

The paper presents the methodology of preparation of samples of oilseeds, lettuce, apples for research by chromatographic control of xenobiotics of the following chemical groups of pesticides: benzimidazole derivatives, anilinopyrimidine derivatives, bipyridylium derivatives. The implementation of the following processes is considered: homogenization of the sample, purification of the extract by solid-phase or liquid-liquid extraction, obtaining a plant extract, obtaining an extract of analytes. For fine-grained homogenized samples of sunflower seeds, the optimal ratio of raw material -extragent is 1:20, for pasty homogenized samples of apple fruit - 1:10, for liquid samples of homogenized lettuce - 1: 5. Analysis of the distribution of xenobiotics in the system octane/water, the dipole moment of solvents allowed to determine the extractants that are able to dissolve and remove xenobiotics from raw materials. It was found that a mixture of acetonitrile and methanol (4: 1) should be used to remove benzimidazole derivatives and anilinopyrimidine derivatives, bipyridylium derivatives are best extracted with methanolic trifluoroacetic acid (9.5: 0.5). Quantitative analysis of xenobiotics content in extracts obtained from samples artificially enriched with xenobiotics was performed. The most complete xenobiotics were removed from samples of plant products containing traces of fat. The most difficult process of sample preparation is the process of obtaining sunflower seed extract. The content of xenobiotics in extracts obtained from samples artificially enriched in analytes is influenced by the temperature at which the process takes place and the duration of extraction. Based on the chemical composition of the sample matrix and the list of analytes, the conditions of the variable component of the methodology are proposed: obtaining plant extract under the action of selective solvents, homogenized raw material-solvent with constant stirring of the extraction system at 180-200 rpm, or under the action of ultrasonic vibrations with a frequency of 37 kHz from 4°C to 25°C for 5-25 minutes. The control of the qualitative and quantitative composition of the studied plant extracts and analyte extracts was investigated by the methods of high-performance liquid and gas chromatography (liquid and gas) with mass-selective detectors.

A novel ultrashort capillary gas chromatography method using on-column injection and detection

2016 ◽  
Vol 94 (4) ◽  
pp. 259-264
Author(s):  
Fadi L. Alkhateeb ◽  
Taylor C. Hayward ◽  
Kevin B. Thurbide
Keyword(s):  
Gas Chromatography ◽  
High Performance ◽  
Chromatography Method ◽  
Gc Analysis ◽  
Chromatography Analysis ◽  
Flame Ionization ◽  
Drug Molecules ◽  
Rapid Temperature ◽  
Capillary Column Gas Chromatography ◽  
Traditional Approaches

A novel method for ultrashort capillary column gas chromatography (GC) analysis is introduced, which employs on-column injection and detection and rapid temperature programming. Using 10–20 cm long capillary columns, results showed that the method provides efficient and very rapid separations for relatively simple mixtures. Moreover, the on-column aspect of the method used here is demonstrated to avoid the extra column analyte degradation that can occur in traditional approaches to such separations. As a result, the developed method allows for the first time the GC analysis of some very large and (or) highly thermally labile analytes, such as polypeptides and drug molecules that are normally prone to decomposition. As an application, this method is further used to monitor pharmaceutical degradant formation as a function of temperature and was found to provide similar results to those obtained from conventional high-performance liquid chromatography analysis. Overall, the findings indicate that this ultrashort GC column approach could be useful in these areas and potentially others, where relatively simple GC analysis and universal flame ionization detection is desirable.

scholarly journals The Impact of Processing Parameters on the Content of Phenolic Compounds in New Gluten-Free Precooked Buckwheat Pasta

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1262 ◽  
Author(s):  
Anna Oniszczuk ◽  
Kamila Kasprzak ◽  
Agnieszka Wójtowicz ◽  
Tomasz Oniszczuk ◽  
Marta Olech
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
High Performance ◽  
Antioxidant Properties ◽  
Total Content ◽  
Natural Antioxidants ◽  
Raw Material ◽  
Gluten Free ◽  
Free Phenolic Acids ◽  
The Impact

Buckwheat is a generous source of phenolic compounds, vitamins and essential amino acids. This paper discusses the procedure of obtaining innovative gluten-free, precooked pastas from roasted buckwheat grains flour, a fertile source of natural antioxidants, among them, phenolic acids. The authors also determined the effect of the extruder screw speed and the level of moisture content in the raw material on the quantity of free phenolic acids. The qualitative and quantitative analysis of phenolic acids in pasta was carried out using high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). The chromatographic method was validated. For extracts with the highest total content of free phenolic acids and unprocessed flour from roasted buckwheat grain, the TLC-DPPH test was also performed to determine the antioxidant properties of the tested pasta. The level of moisture in the raw material had an impact on the content of phenolic acids. All pastas made from buckwheat flour moistened up to 32% exhibited a higher total content of free phenolic acids than other mixes moistened to 30 and 34% of water.

scholarly journals Comparative Analysis of Phenolic Compounds in Seven Hypericum Species and Their Antioxidant Properties

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Gordana Zdunic ◽  
Dejan Godjevac ◽  
Katarina Savikin ◽  
Silvana Petrovic
Keyword(s):  
Comparative Analysis ◽  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Uv Spectra ◽  
Biologically Active ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Hypericum Species

A comparative analysis of the phenolic compounds in the 70% EtOH extracts of Hypericum acutum, H. androsaemum, H. barbatum, H. hirsutum, H. maculatum, and H. richeri has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and high resolution TOF mass spectrometry. Quercetin, astilbin, I3, II8-biapigenin, orientin, 2”- O-acetylorientin, three phenolcarboxylic acids, and eight flavonols 3- O-glycosides were identified in the extracts on the basis of their on-line UV spectra, accurate mass spectral data, and in comparison of retention times with those from the standards. Fingerprint analysis of the extracts revealed significant differences in the qualitative and quantitative chemical composition of the studied species. Antioxidant assays with various reaction mechanisms were used including ferric reducing antioxidant power (FRAP) assay, DPPH, ABTS, superoxide anion radical scavenging capacity and inhibition of liposome peroxidation induced by Fe2+. The most potent were extracts of H. acutum and H. maculatum indicating this Hypericum species interesting for further research aimed as a potentially new source of biologically active compounds.

Characterization of phenolic compounds from inner bark of Betula pendula

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Jaana Liimatainen ◽  
Maarit Karonen ◽  
Jari Sinkkonen ◽  
Marjo Helander ◽  
Juha-Pekka Salminen
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Betula Pendula ◽  
Silver Birch ◽  
Biologically Active ◽  
Array Detector ◽  
Ionization Mass ◽  
Inner Bark ◽  
First Time

Abstract A method has been developed for the characterization of biologically active silver birch (Betula pendula) inner bark phenolics based on high-performance liquid chromatography/diode array detector (HPLC-DAD)/electrospray ionization-mass spectrometry (ESI-MS). It was demonstrated that the inner bark contains high amounts of flavonoids, arylbutanoids, diarylheptanoids, simple phenolic compounds, phenolic acids, lignans, and procyanidins. Altogether, 30 individual compounds were characterized based on their ultraviolet (UV) and MS data. Structures of 22 compounds were confirmed by nuclear magnetic resonance (NMR) spectroscopy. In addition to previously reported phenolic compounds, 12 compounds were identified in silver birch inner bark for the first time; two of them are novel compounds: 3-β-glucopyranosyloxy-2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-propan-1-one and 1,7-bis-(4-hydroxyphenyl)-3-heptanol 3-O-β-ap-iofuranosyl-(1→2)-β-glucopyranoside.

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