scholarly journals OMPARATIVE STUDIES OF THE CONTENT OF PHENOLIC COMPOUNDS, FLAVONOIDS AND ANTIOXIDANT ACTIVITY OF APPLES OF DIFFERENT VARIETIES

2018 ◽  
pp. 115-122 ◽  
Author(s):  
Надежда (Nadezhda) Викторовна (Viktorovna) Макарова (Makarova) ◽  
Динара (Dinara) Фанисовна (Fanisovna) Валиулина (Valiulina) ◽  
Олег (Oleg) Игоревич (Igorevich) Азаров (Azarov) ◽  
Анатолий (Anatolij) Александрович (Aleksandrovich) Кузнецов (Kuznetsov)
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Phenolic Compounds ◽  
Acid System ◽  
Antioxidant Ability ◽  
Restoring Force ◽  
Antioxidant Power ◽  
Hydroalcoholic Extract ◽  
System A ◽  
Ferric Reducing Antioxidant Power

In this article discusses the most commonly used methods of investigation of antioxidants.  Six varieties of apples (23-25-64, 23-17-22, 23-20-50, 23-25-51, 23-31-135, Sinap of Samara) were selected as objects of research, from the collection of the Research Institute "Zhigulevsky Gardens" "The harvest of 2016 grown on the territory of the Samara region. For the study was obtained hydroalcoholic extract (1 : 1) at 37 °C for 2 hours. The analysis for the maintenance of the total number of phenolic compounds content of gallic acid, flavonoids catechin content, antioxidant capacity with use of free radical DPPH (2,2-diphenyl-1-pikrilgidrazil), restoring forceb with method of FRAP (ferric reducing antioxidant power), the antioxidant activity in linoleic acid system. A significant number of methods to assess the antioxidant activity of the compounds according to the method systematically recording parameters used to determine antioxidant activity, including quantitative. As a result of a comparative study of the content of phenolic compounds, flavonoids, antiradical ability using the 2,2'-diphenyl-1-picrylhydrazyl reagent method, the FRAP restoring force with 2,4,6-tri(2-pyridyl)-1,3 reagent, 5-triazine, antioxidant activity on the linoleic acid model for six varieties of apples were identified as leaders by individual indicators (phenols - apples of variety 23-20-50, flavonoids - apples of variety 23-20-50, anti-radical ability – apples of variety 23-25-51, FRAP-values – apples of variety 23-20-50, antioxidant ability – Syn up of Samara), and in general for the majority of indicators - apples of variety 23-20-50.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Phenolic Content, Antioxidant and 15-Lypoxygenase Inhibitory Effects of Daniellia oliveri (rolfe) Hutch. & Dalziel (Fabaceae)

2021 ◽  
pp. 10-18
Author(s):  
Mariam Traore ◽  
Adjaratou C. Coulibaly ◽  
Aboubacar Yaro ◽  
Esther W. L. M. Kabre ◽  
Noufou Ouedraogo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Phytochemical Screening ◽  
Methanol Fraction ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Daniellia Oliveri

Aims: The aim of this study is to evaluate the content of phenolic compounds, antioxidant and 15-lipoxygenase inhibition effects of methanolic extract and its fractions of Daniellia. oliveri stem bark. Place and Duration of Study: The work was carried out in the Department of Traditional Pharmacopoeia and Pharmacy (MEPHATRA / PH) of the Institute for Research in Health Sciences (IRSS) and LABIOCA from Université Joseph KI-ZERBO in Ouagadougou between February 2018 and June 2019 Methodology: The phytochemical screening and phenolic content were determined by the methods described in the literature. Antioxidant activity was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,20-azinobis 3-ethylbenzoline-6-sulphonate), FRAP (ferric reducing antioxidant power), and lipid peroxidation (LPO). The anti-inflammatory activities were measured on the ability of the extract to inhibit the activity of enzymes such as 15-lipoxygenase Results: Phytochemical screening revealed the presence of flavonoids, tannins, saponins, anthocyanosides, triterpenes and sterols. The extract and its fractions showed a good content of phenolic compounds with values ranging from 773.97 ± 22.91 to 505.51 mg EAG / g for total polyphenols; from 24.38 ± 0.34 to 12.04 ± 0.08 mg EQ / g in total flavonoids, 311.65 ± 6.70 to 221 ± 0.52 mg EC / g in condensed tannins; 681.81 ± 3.97 to 421.29 ± 8.73 mg EUA / g in total triterpenes and from 280.29 ± 21.72 to 222.04 ± 20.99 mg EC / g for total sterols. The methanol fraction showed the best antioxidant activity as well as the best inhibitory effect on 15-lipoxygenase activity. Conclusion: These results showed that Daniellia. oliveri possessed an antioxidant power, an inhibitory effect of 15 lipoxygenase. Daniellia oliveri could be used in the management of inflammatory pathologies and oxidative stress.

scholarly journals Experimental approaches targeting the biochemical and antioxidant potential of Synsepalum dulcificum dried fruits

2021 ◽  
Vol 26 (1) ◽  
pp. 2230-2235
Author(s):  
GABRIELA POPA ◽  
RADU TOMA ◽  
JEAN-FRANÇOIS BOÉ
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Dry Matter ◽  
Natural Antioxidants ◽  
Fruit Pulp ◽  
Antioxidant Power ◽  
Dried Fruits ◽  
Dried Fruit ◽  
Ferric Reducing Antioxidant Power ◽  
Pharmacologically Active

This study aimed to investigate the biochemical content and antioxidant activity of methanol extracts prepared from Synsepalum dulcificum dried fruits. The results obtained by phytochemical screening of the extracts revealed the presence of some pharmacologically active chemical compounds such as: alkaloids, flavonoids, phenolic compounds and tannins. Quantitative analysis showed that the seeds had phenols in greater amounts (157.88 ± 2.09 mg GAE / g dry matter) than dried fruit pulp (54.09 ± 2.24 mg GAE / g dry matter). Contrariwise, flavonoid content was found to be low in seed (0.013 ± 0.02 mg EQ / g) and high in fruit pulp (0.13 ± 0.05 mg EQ / g). Correlated with the high amount of total phenol, in the seed extracts was found a good antioxidant activity – measured by both DPPH and ferric reducing antioxidant power (FRAP) assays. This antioxidant capacity may be also correlated with the amount of alkaloids, flavonoids, tannins and phenolic compounds, and as well as with their synergistic interactions. Synsepalum dulcificum fruits can be used as a healthy source of additives for the food industry or as natural antioxidants for the pharmaceutical industry.

scholarly journals Comparative Phytochemical Profile and Biological Activity of Four Major Medicinal Halophytes from Qassim Flora

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2208
Author(s):  
Hamdoon A. Mohammed ◽  
Hussein M. Ali ◽  
Kamal A. Qureshi ◽  
Mansour Alsharidah ◽  
Yasser I. Kandil ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Biological Properties ◽  
Human Pancreatic Cancer ◽  
Antioxidant Power ◽  
Hydroalcoholic Extract ◽  
Anabasis Articulata ◽  
Ic50 Values ◽  
Ferric Reducing Antioxidant Power ◽  
Induced Apoptosis

Four halophytic plants, Lycium shawii, Anabasis articulata, Rumex vesicarius, and Zilla spinosa, growing in the central Qassim area, Saudi Arabia, were phytochemically and biologically investigated. Their hydroalcoholic extracts’ UPLC-ESIQ-TOF analyses demonstrated the presence of 44 compounds of phenolic acids, flavonoids, saponins, carbohydrates, and fatty acids chemical classes. Among all the plants’ extracts, L. shawii showed the highest quantities of total phenolics, and flavonoids contents (52.72 and 13.01 mg/gm of the gallic acid and quercetin equivalents, respectively), along with the antioxidant activity in the TAA (total antioxidant activity), FRAP (ferric reducing antioxidant power), and DPPH-SA (2,2-diphenyl-1-picryl-hydrazyl-scavenging activity) assays with 25.6, 56.68, and 19.76 mg/gm, respectively, as Trolox equivalents. The hydroalcoholic extract of the L. shawii also demonstrated the best chelating activity at 21.84 mg/gm EDTA equivalents. Among all the four halophytes, the hydroalcoholic extract of L. shawii exhibited the highest antiproliferative activity against MCF7 and K562 cell lines with IC50 values at 194.5 µg/mL and 464.9 µg/mL, respectively. The hydroalcoholic extract of A. articulata demonstrated better cytotoxic activity amongst all the tested plants’ extracts against the human pancreatic cancer cell lines (PANC1) with an IC50 value of 998.5 µg/mL. The L. shawii induced apoptosis in the MCF7 cell lines, and the percentage of the necrotic cells changed to 28.1% and 36.5% for the IC50 and double-IC50 values at 22.9% compared with the untreated groups. The hydroalcoholic extract of L. shawii showed substantial antibacterial activity against Bacillus cereus ATCC 10876 with a MIC value of 12.5 mg/mL. By contrast, the A. articulata and Z. spinosa exhibited antifungal activities against Aspergillus niger ATCC 6275 with MIC values at 12.5 and 50 mg/mL, respectively. These findings suggested that the L. shawii is a potential halophyte with remarkable biological properties, attributed to its contents of phenolics and flavonoid classes of compounds in its extract.

scholarly journals Blackberry (Rubus spp.): influence of ripening and processing on levels of phenolic compounds and antioxidant activity of the 'Brazos' and 'Tupy' varieties grown in Brazil

2015 ◽  
Vol 45 (4) ◽  
pp. 744-749 ◽  
Author(s):  
Acácio Antonio Ferreira Zielinski ◽  
Caroline Goltz ◽  
Maira Akemi Casagrande Yamato ◽  
Suelen Ávila ◽  
Elisa Yoko Hirooka ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Anthocyanin Content ◽  
Antioxidant Power ◽  
Significant Difference ◽  
Ferric Reducing Antioxidant Power ◽  
Fermented Beverage ◽  
Tropical Climates

Fruits from temperate and tropical climates which have high levels of antioxidant compounds are the source of numerous studies concerning the correlation with benefits to human health. The objectives of this study were to quantify the anthocyanins and phenolic compounds and also to measure the antioxidant activity (ferric reducing antioxidant power - FRAP) of blackberries from two varieties grown in southern Brazil ('Brazos' and 'Tupy') at three stages of ripening; unripe, semi-ripe, ripe and their products (pulp and fermented products). During fruit ripening it was observed that weight, size, diameter and sugars increase significantly and acidity decreased significantly. The anthocyanin content ranged from 4.19 (semi-ripe 'Tupy' variety) to 205.75mg 100g-1 (ripe 'Brazos' variety). The highest levels of phenolic compounds were observed for the unripe fruit of both varieties, while antioxidant activity showed no significant difference during the ripening stages. The studied pulp showed a high content of phenolic compounds (ten times higher than that found in the ripe fruits). The anthocyanin content and antioxidant activity did not show the same increase due to the degradation of anthocyanins caused by the heat treatment that was used. The alcoholic fermented beverage made from blackberries remained stable (total phenolic compounds and antioxidant activity) during two years of storage, but the in third year a significant reduction in antioxidant activity was observed. These results can be important for establishing the shelf life of this kind of product made with blackberry

scholarly journals Content of Phenolic Compounds in Various Sea Buckthorn Parts

2013 ◽  
Vol 67 (4-5) ◽  
pp. 411-415 ◽  
Author(s):  
Elga Šne ◽  
Dalija Segliņa ◽  
Ruta Galoburda ◽  
Inta Krasnova
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sea Buckthorn ◽  
Raw Material ◽  
Antioxidant Power ◽  
Spectrophotometric Methods ◽  
Ferric Reducing Antioxidant Power ◽  
Medicinal Properties

Abstract All parts of the sea buckthorn (Hippophae rhamnoides L.) plant are considered to contain large amounts of compounds that are believed to have beneficial health effects. Till now, different parts of sea buckthorn plant have been used for the treatment of diseases in traditional medicine in various countries. Nevertheless, sea buckthorn parts would be a good raw material not only for medicinal properties but also for food products with functional properties; therefore, the aim of the research was to determine the concentration of different phenolic compounds and antioxidant activity in various sea buckthorn parts. The study was conducted on parts of female and male sea buckthorn bushes. Phenolic compounds (total phenols, total flavonoids and condensed tannins) and antioxidative activity (ferric reducing antioxidant power (FRAP) free radical scavenging activity (using 2.2-diphenyl-1-picrylhydrazyl, DPPH)) in ethanolic extracts of leaves, shoots, flowers, and berries were determined using various spectrophotometric methods. The study showed that concentration of phenolic compounds differed among parts of sea buckthorn plant and among gender. Leaves of female plants proved to be the most valuable, as they contained the highest total phenol concentration (165.76 mg/g) and antioxidant activity (220.97 mg/g for FRAP and 43.76 mg/g for DPPH), while lowest values were found in young shoots of male plants (84.94 mg/g, 94.24 mg/g and 24.63 mg/g, respectively). The significant differences in chemical composition and biological activity of sea buckthorn leaves, shoots, berries, and buds indicate a need for detailed studies of their extracts, specific fractions and compounds during a whole vegetative season.

scholarly journals Comparison of phenolic profiles and antioxidant activity of three Ornithogalum L. species

2018 ◽  
Vol 44 (3) ◽  
pp. 299-306
Author(s):  
Gülin Renda ◽  
Arzu Özel ◽  
Emine Akyüz Turumtay ◽  
Burak Barut ◽  
Büşra Korkmaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Protocatechuic Acid ◽  
Radical Scavenging ◽  
Coumaric Acid ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Phenolic Profiles ◽  
Ferric Reducing Antioxidant Power ◽  
First Time

Abstract Background Aboveground parts and bulbs of Ornithogalum species are consumed as food and used in traditional medicine in worldwide. Objective It is aimed to report the antioxidant capacity and phenolic compounds content of Ornithogalum sigmoideum, Ornithogalum orthophyllum and Ornithogalum oligophyllum for the first time. Materials and methods Antioxidant activity of the crude methanol extracts of the aerial parts and the bulbs of the species were determined with 1,1-diphenyl-2-picryl-hydrazyl, superoxide radical scavenging, ferrous ion-chelating effect, phosphomolybdenum-reducing antioxidant power and ferric-reducing antioxidant power assay. The ethylacetate, diethylether and water subextracts from leaf and flower were analyzed to quantify selected phenolic compounds by HPLC-UV. Results Among the six extracts, the methanol extract of the aerial parts of O. orthophyllum contained the highest amount of phenolic compounds (GAE, 11.0 mg/g extract). The aerial parts of O. orthophyllum showed higher DPPH and SOD activities than the other extracts with the SC50 values of 0.39±0.05 mg/mL and 0.44±0.08 mg/mL, respectively. Protocatechuic acid, p-hydroxybenzoic acid, vanillic acid and p-coumaric acid were the most abundant compounds at all the subextracts. Conclusion The antioxidant activity is found to be in accordance with the levels of phenolic content in the extracts.

scholarly journals Variation in Phenolic Compounds Content and Antioxidant Activity of Different Plant Organs from Rumex crispus L. and Rumex obtusifolius L. at Different Growth Stages

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 237 ◽  
Author(s):  
Pavel Feduraev ◽  
Galina Chupakhina ◽  
Pavel Maslennikov ◽  
Natalia Tacenko ◽  
Liubov Skrypnik
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Reproductive Organs ◽  
Biologically Active ◽  
Total Phenolic ◽  
Growth Stages ◽  
Active Components ◽  
Rumex Crispus ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The study investigated the accumulation of phenolic compounds and the antioxidant activity of extracts of various parts of R. crispus and R. obtusifolius, collected at the flowering stage and the fruiting stage. Half of the collected plants were divided into root, stem, leaves, and reproductive organs (inflorescence). The other half was used to study the vertical distribution of biologically active components and antioxidants throughout the plant. The samples were analyzed for total catechins content, total proanthocyanidins content, total phenolic content, and total antioxidant activity (1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’azinobis(3)ethylbenzthiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays). All analyses were performed in four replicates. In general, a similar trend was observed in the distribution of phenolic compounds in the studied species. The maximum content of these secondary metabolites was noted in the reproductive organs, both in the flowering and fruiting period. Stems were characterized by a minimum content of the studied classes of substances. The antioxidant activity of the sorrels studied parts can be arranged in the following order: the generative part (flowers, seeds) > leaves > root > stem (for flowering and fruiting stages). It was found that parts of the root closer to the stem differed in higher activity.

scholarly journals Chemical Composition of Essential Oil, Phenolic Compounds Content, and Antioxidant Activity of Cistus monspeliensis from Northern Morocco

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Sara Haida ◽  
Kaltoum Bakkouche ◽  
Abdelaziz Ramadane Kribii ◽  
Abderahim Kribii
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Condensed Tannins ◽  
Natural Antioxidants ◽  
Health It ◽  
Total Polyphenols ◽  
Antioxidant Power ◽  
Liquid Liquid Extraction ◽  
Ferric Reducing Antioxidant Power

Currently, oxidative stress is one of the major problems that threatens human health. It is at the root of many diseases such as cancer. Despite the enormous efforts provided to combat this scourge, oxidative stress is still relevant and hence comes the need for research of new remedies especially from natural origin. For this purpose, the study of the antioxidant activity of extracts of Cistus monspeliensis from Morocco is a principal research objective. The phenolic extracts were obtained by maceration of the plant in a water/acetone mixture and then separated by liquid/liquid extraction with solvents of increasing polarity. The first phytochemical tests carried out on these extracts showed the existence of different families of phenolic compounds, such as flavonoids, tannins, and others. Assays for total polyphenols, flavonoids, hydrolysable, and condensed tannins were carried out by known colorimetric methods. The results of these assays have shown that the studied extracts are rich in phenolic compounds present in the plant in the form of flavonoids (69.81 ± 0.22 mg EQ/g DM), hydrolysable tannins (61.86 ± 0.89 mg ETA/g DM), and condensed tannins (70.05 ± 1.61 mg EC/g DM). The evaluation of the antioxidant activity is carried out by two different methods: the DPPH test (2,2-DiPhenyl-1-Picryl-Hydrazyl) and the FRAP test (Ferric Reducing Antioxidant Power). The results obtained show that the extracts of Cistus monspeliensis are active and have interesting antioxidant powers. In particular, the water/acetone (WAE) (IC50 = 0.079 mg/mL) and butanolic (BUE) (IC0.5 = 0.099 mg/mL) extracts are the most active with values comparable to that of ascorbic acid. The interesting results obtained in this study clearly show that Cistus monspeliensis originating from Morocco can be considered as a source of natural antioxidants. Therefore, the extracts of this plant deserve to be tested in the medicinal field, against cancer and cardiovascular diseases, and in food field as an additive and preservative.

scholarly journals Differential Antioxidant Compounds and Activities in Seedlings of Two Rice Cultivars Under Chilling Treatment

2021 ◽  
Vol 12 ◽  
Author(s):  
Shangguang Du ◽  
Xueyong Huang ◽  
Yali Cai ◽  
Yingbin Hao ◽  
Shengrong Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
High Performance ◽  
Food Choices ◽  
Antioxidant Compounds ◽  
Rice Cultivars ◽  
Treatment Results ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Chilling Treatment

Variations in antioxidant compounds were examined in seedlings of two rice cultivars (Qiutianxiaoting and 93-11) exposed to low temperature (4°C) for 0, 12, 36, and 48 h. Antioxidant activity was identified by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The concentrations of total phenols, flavonoids, chlorophyll, and anthocyanins (ACNs) were determined by spectrophotometry. In addition, high-performance liquid chromatography (HPLC) was used to reveal the changes in phenolic compound concentrations in rice seedlings under chilling treatment. Results showed that antioxidant concentrations and antioxidant activity after chilling treatment were higher in 93-11 compared to Qiutianxiaoting, reaching the highest level at 36 h chilling treatment in 93-11. Phenolic compounds in Qiutianxiaoting decreased between 12 and 36 h but then increased at 48 h, whereas the corresponding levels in 93-11 increased as chilling time increased. Moreover, 10 phenolic compounds were detected and quantified by HPLC, of which gallic acid and caffeic acid tended to only exist in 93-11, whereas rutin was observed only in Qiutianxiaoting. The results of this study could be leveraged to optimize the antioxidant potential of rice in the context of healthy food choices.

Sign in / Sign up

Export Citation Format

Share Document