scholarly journals Antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia Against Enterococcus Feacalis by Agar Well Diffusion Method and Minimal Inhibitory Concentration- An Invitro Study.

2019 ◽  
Vol 22 (3) ◽  
pp. 365-370
Author(s):  
Sriram Gummuluri ◽  
Venkata Teja Kavalipurapu ◽  
Apoorva Vasundhara Kaligotla
Keyword(s):  
Antimicrobial Activity ◽  
Minimal Inhibitory Concentration ◽  
Root Canal ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
The Novel ◽  
Agar Well Diffusion Assay

Introduction: the long term success of root canal treatment is ultimately related to the effective debridement and disinfection of the root canal system. Hence, the irrigants play an important role in achieving the good penetrability and bactericidal activity. The present study was mainly aimed at evaluating the invitro antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia by agar well diffusion and minimal inhibitory concentration. Materials and Methods:the antimicrobial efficacy of the Novel Ethanolic Extract of Morinda Citrifolia was tested using agar well diffusion and minimal inhibitory concentration was assessed. The zone of inhibitions were determined at 10 mg/ml concentration of Ethanolic Extract of Morinda Citrifolia on agar well plate and Minimal Inhibitory Concentration (MIC) against tested microorganism. Results: results obtained in the present study by both qualitative and quantitative experiments revieled that the tested Ethanolic Extract of Morinda Citrifolia possesses potential antibacterial activity against Enterococcus Feacalis when compared with standard antibiotic tetracycline. the highest zone of inhibition of 15mm was showed at 1000micrograms by agar well diffusion assay. The optimal antimicrobial activity was seen at 250micrograms for Morinda Citrifolia against Enterococcus Feacalis.Conclusion: novel Ethanolic extract of Morinda Citrifolia has shown an optimal antimicrobial activity against E.Feaclais. But still, future studies are still needed.

scholarly journals Antimicrobial Activity of Leaves and Flowers of Cassia auriculata linn

1970 ◽  
Vol 46 (4) ◽  
pp. 513-518 ◽  
Author(s):  
V Subhadradevi ◽  
K Asokkumar ◽  
M Umamaheswari ◽  
AT Sivashanmugam ◽  
JR Ushanandhini ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Dominant Role ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Cassia Auriculata ◽  
Potential Source ◽  
Wide Range ◽  
Ancient Times

Since ancient times plant as sources of medicinal compounds have continued to play a dominant role in the maintenance of human health. To treat chronic and infectious diseases plants used in traditional medicine contain a wide range of ingredients. In this regard, Cassia auriculata L. (Caesalpiniaceae) is widely used in Ayurvedic medicine as a tonic, astringent and as a remedy for diabetes, conjunctivitis, ulcers, leprosy, skin and liver diseases. The aim of present study was to evaluate the antimicrobial activity of ethanolic extract of Cassia auriculata leaves and flowers (CALE & CAFE). CALE and CAFE exhibited broad spectrum antimicrobial activity against standard strains of Staphylococcus aureus, Escherichia coli and Bacillus subtilis and exhibited no antifungal activity against standard strains of Candida albicans and Aspergillus niger. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) was carried out for CALE and CAFE. The results obtained in the present study indicate that the CALE and CAFE can be a potential source of natural antimicrobial agents. Key words: Cassia auriculata; Antimicrobial activity; Agar well diffusion method. DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9600 BJSIR 2011; 46(4): 513-518

Comparing the Antimicrobial efficacy of Dentifrice containing Rosmarinus officinalis and Fluoride containing Dentifrice - An In vitro study

2021 ◽  
pp. 3651-3656
Author(s):  
Indumathy Pandiyan ◽  
Jayashri Prabakar ◽  
Meignana Arumugham Indiran ◽  
Pradeep Kumar Rathinavelu ◽  
Srisakthi Doraikannan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Herbal Medicine ◽  
Enterococcus Faecalis ◽  
Antimicrobial Property ◽  
Ethanolic Extract ◽  
Rosmarinus Officinalis ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Dental Biofilm

Background: Dental biofilm is still a concern for dental professionals, given that it has been correlated with the aetiology of oral diseases. Herbal medicine or plant derived extracts are considered to be safer and exhibits less or no adverse effects in comparison with chemically synthesized medicines. Hence, herbal medicine and their extract can be used as an adjuvant in dental disease treatment. One among the various plant extract exhibiting antimicrobial activity against oral microorganisms is Rosemarinus officinalis is an aromatic plant belonging to lamiaceae family originating from Mediterranean region. Therefore the present study was designed to assess the antimicrobial efficacy of Rosmarinus officinalis and Fluoride containing dentifrices against streptococcus aureus, Enterococcus Faecalis and Candida albicans. Materials and Method: Rosemary leaves were procured from the markets of South India and were powdered to prepare the aqueous and ethanolic extract of Rosmarinus Officinalis. Both the extracts of Rosmarinus Officinalis and Fluoride containing dentifrice were subjected to antimicrobial analysis. The antimicrobial activity was evaluated using Agar well diffusion method. The diameters of the zones of inhibited growth were measured to the nearest whole millimetre. Data was entered in Microsoft excel spread sheet and analysed using SPSS software (version 23). Descriptive statistics were expressed by means of percentage. Results: The antimicrobial activity of Ethanolic extract of Rosmarinus officinalis at 100 conc. showed zone of inhibition of 20mm, 22mm, 17mm against Streptococcus mutans, Enterococcus faecalis and Candida albicans respectively. Hence the Rosemary toothpaste had good antimicrobial property when compared to fluoride toothpaste. Conclusion: Based on the results of the present study, it can be concluded that, rosemary containing dentifrice exhibited good antimicrobial properties when compared with fluoride dentifrice and it can be serve as an excellent replacement of several commercial available dentifrices.

scholarly journals ANTIMICROBIAL ACTIVITY OF MIXTURE FROM DIFFERENT PLANT EXTRACTS IN GEL, CREAM AND BODY MILK FORMULATION, AGAINST DIFFERENT MICROORGANISMS

2017 ◽  
Vol 35 (2) ◽  
Author(s):  
Dzoko Kungulovski ◽  
Natalija Atanasova-Pancevska
Keyword(s):  
Antimicrobial Activity ◽  
Minimal Inhibitory Concentration ◽  
Plant Extracts ◽  
Broad Spectrum ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Inhibition Zones ◽  
Standard Disc

In this study the antimicrobial activity of a mixture of plant extracts originating from five different plants and suspended in three different forms of gel, cream and lotion was examined with the purpose of discovering new anti-microbial compounds. The antimicrobial activity was investigated through the standard disc diffusion method, as well as through a variation of the microdillution method. The formulations under examination (gel, cream and lotion) showed a broad spectrum of action against all the selected microorganisms, with inhibition zones of 9–46 mm. The minimal inhibitory concentration (MIC) for all the formulations against the microorganisms in the study was in the range of 0.000761μg ml-1 to 0.125μg ml-1. The results of this study have clearly demonstrated that the mixture of plant extracts originating from five different plants and suspended in the forms of gel, cream and lotion can definitely be used in the battle against the microorganisms under investigation.

scholarly journals The synthesis, antimicrobial activity and docking studies of 6-(1H-benzimidazol-2-yl)-5-methylthieno[2,3-d]pyrimidin- 4(3H)-ones with acetamide and 1,2,4-oxadiazol-5-ylmethyl substituents

2021 ◽  
Vol 19 (3(75)) ◽  
pp. 15-20
Author(s):  
Sergiy V. Vlasov ◽  
Oleksandr V. Borysov ◽  
Hanna I. Severina ◽  
Sergiy M. Kovalenko ◽  
Tetiana P. Osolodchenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ribosomal Rna ◽  
Active Site ◽  
Docking Studies ◽  
Binding Activity ◽  
Diffusion Method ◽  
The Novel ◽  
Reference Drug ◽  
Sabouraud Agar ◽  
Agar Well Diffusion Assay

Aim. To synthesize, study the antimicrobial activity and suggest antimicrobial activity mechanism for the novel derivatives of 6-(1H-benzimidazol-2-yl)-5-methylthieno[2,3-d]pyrimidin-4(3H)-one. Results and discussion. As the result of the targeted modification of 6-(1H-benzimidazol-2-yl)-5-methylthieno[2,3-d]-pyrimidin-4(3H)-one in position 3 with acetamide and 1,2,4-oxadiazol-5-ylmethyl substituents, the compounds, which demonstrated better antimicrobial activity in the agar well diffusion assay than the reference drug Streptomycin, were obtained. To elucidate the mechanism of action of the novel compounds, the docking studies were con-ducted to the active site of the 16S subunit of ribosomal RNA, the proven target for aminoglycoside antibiotics, as well as tRNA (Guanine37-N1)-methyltransferase (TrmD), which inhibitors were considered as a new potential class of antibiotics. Experimental part. By the interaction of 6-(1H-benzimidazol-2-yl)-5-methylthieno[2,3-d]pyrimidin-4(3H)-one with a series of N-arylchloroacetamides and 3-aryl-5-(chloromethyl)-1,2,4-oxadiazoles in DMF in the presence of K2CO3 the target compounds were obtained. The antimicrobial activity was assessed by the agar well diffusion method. The concentration of microbial cells was determined by the McFarland standard; the value was 107 cells in 1 mL of the media. The 18 – 24 hour culture of microorganisms was used for tests. For the bacteria cultivation, Müller-Hinton agar was used, Sabouraud agar was applied for C. albicans cultivation. The compounds were tested as the DMSO solution with the concentration of 100 µg/mL; the volume of the solution was 0.3 mL, the same volume was used for Streptomycin (the concentration 30 µg/mL). The docking studies were performed using Autodock Vina. Crystallographic data for the complexes of Streptomycin with the 16S subunit of ribosomal RNA (1NTB) and its active site, as well as for tRNA (Guanine37-N1)-methyltransferase (EC 2.1.1.228; TrmD) (5ZHN) and its active site were obtained from the Protein Data Bank.Conclusions. It has been determined that 2-[6-(1H-benzimidazol-2-yl)-5-methyl-4-oxothieno[2,3-d]pyrimidin-3(4H)-yl]-N-[4-(ethoxy)phenyl]acetamide, which is the most active as an antimicrobial agent among the compounds tested, also shows the best binding activity towards the active site of tRNA (guanine37-N1)-methyltransferase.

scholarly journals ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ETHANOLIC LEAF EXTRACTS OF ANACARDIUM OCCIDENTALE

2018 ◽  
Vol 11 (12) ◽  
pp. 474
Author(s):  
Shobha Kl ◽  
Amita Shobha Rao ◽  
Pai Ksr ◽  
Sujatha Bhat
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Klebsiella Pneumoniae ◽  
Bacterial Infections ◽  
Ethanolic Extract ◽  
Culture Collection ◽  
Anacardium Occidentale ◽  
Diffusion Method ◽  
Leaf Extracts

Objective: The objective of this study was to evaluate the antimicrobial activity of leaves of Anacardium occidentale (A. occidentale) against microorganisms including multidrug-resistant (MDR) bacteria. Methods: Agar well diffusion method was employed to demonstrate the antimicrobial activity of leaves A. occidentale. Ethanol and aqueous extracts of the leaves were used against microorganisms, which included American type culture collection strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa and the clinical isolates of Streptococcus pneumoniae, Candida albicans, MDR Escherichia coli, and MDR Klebsiella pneumoniae. Results: The ethanolic extract of leaves of A. occidentale showed significant antimicrobial activity. Aqueous extract had mild antifungal activity. Conclusion: Ethanolic extract of leaves of A. occidentale could be a good source for the antibacterials to combat MDR bacterial infections. Further studies are necessary for these potent plant extracts to evaluate the in vivo efficacy and toxicity.

scholarly journals Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

2010 ◽  
Vol 7 (3) ◽  
pp. 1159-1165
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Cold Water ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Diffusion Method ◽  
Resistant Bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds

scholarly journals A Comparative Study of Antimicrobial Activity of Ethanolic Extract of Tecoma stans and Abutilon indicum Leaves: An In vitro Study

2021 ◽  
pp. 609-615
Author(s):  
M. Binigha ◽  
R. Gayatri Devi ◽  
J. Selavaraj ◽  
A. Jothi Priya
Keyword(s):  
Antimicrobial Activity ◽  
Agar Plate ◽  
Ethanolic Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
The Family ◽  
Abutilon Indicum

Tecoma stans is a flowering plant belonging to the family Bignoniaceae. It is the floral emblem of the Bahamas. Abutilon indicum belongs to the family Malvaceae. It is also called Indian Mallow. Tecoma stans and Abutilon indicumhave antimicrobial activity and can be used in producing antibiotics. There is an urgent need to produce new antibiotics as there is an increase in the development of pathogen resistant drugs. The aim of this study was to compare the antimicrobial activity of ethanolic extract of Tecoma stansand Abutilonindicum leaves. E.faecalis bacteria were maintained in nutrient agar slopes. The powder of the two herbs, were subjected to extraction by Soxhlet extractor and was concentrated to dry residue by Agar well diffusion method. The nutrient broth was inoculated with bacterial strains. The culture was adjusted to 0.5McFarland turbidity standard. Lawn culture was done in Muller-Hinton agar plate and then the plates were dried. A 6 mm diameter well is bored for different concentrations. The extracts were introduced into the well and allowed to stand for 24 hrs. The antibacterial activity was determined by measuring the zone of inhibition. The present finding on the antimicrobial potential of Abutilon indicum and Tecoma stands showed that the two plants have growth inhibitory activity against oral pathogen E. faecalis. Abutilon indicum has a zone of inhibition greater than Tecoma stans. Abutilon indicum exhibited better activity compared to Tecoma stans.

Antimicrobial Activity of N,N'-bis(decylmethyl)- α,ω-alkanediamine Dioxides [1]

1983 ◽  
Vol 38 (1-2) ◽  
pp. 151-152 ◽  
Author(s):  
Ferdinand Devínsky ◽  
Ivan Lacko ◽  
Ludovít Krasnec ◽  
Dušan Mlynarčík
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration

Antimicrobial activity of N,N′-bis(decylmethyl)-α,ω-alkanediamine dioxides determined on Staphylococcus aureus, Escherichia coli, and Candida albicans is presented as minimal inhibitory concentration (MIC). The effect of the length of linking alkylene chain on this activity has been followed.

Antimicrobial activity of Syzygium caryophyllatum L.

2021 ◽  
pp. 4275-4276
Author(s):  
Rohit Raj ◽  
Chandrashekar. K. S ◽  
Vasudev Pai
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Small Tree ◽  
Good Antibacterial Activity ◽  
Tropical Area ◽  
The Family ◽  
Agar Diffusion Method ◽  
India And China

Syzygium caryophyllatum L. is a small tree or large shrub grow widely mainly in the tropical area. It is native to India and China. S. caryophyllatum L. belonging to the family Myrtaceae is taken for screening antimicrobial activity. Ethanolic extract of the leaves of Syzygium caryophyllatum was screened for antibacterial activity using Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Activity of ethanolic extract was good when compared to Ampicillin. The antimicrobial activity was determined by Agar diffusion method and also MIC technique. From the result it was found that ethanolic extract of the leaves of Syzygium caryophyllatum exhibited good antibacterial activity against these gram +ve and gram –ve microorganisms.

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

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