scholarly journals DESARROLLO DE MODELO MURINO DE COLONIZACIÓN DE Lactobacillus plantarum LP5 DE ORIGEN PORCINO

2021 ◽  
Vol 19 (suplemento) ◽  
Author(s):  
M J Ruiz
Keyword(s):  
Escherichia Coli ◽  
Mus Musculus ◽  
Clinical Signs ◽  
Inhibitory Effect ◽  
Microbiological Analysis ◽  
General State ◽  
The Third ◽  
Ad Libitum ◽  
And Control ◽  
Lactobacillus Spp

The objective of this work was to generate an experimental model of colonization by L. plantarum LP5 in mice (Mus musculus) aimed at improving the general state of the animal and preventing the possible presence of zoonotic pathogens. The mice used were females of the Balb/cCmedc strain, 6 weeks old. Animals were organized into 2 groups of 3 individuals: treated (GT) and control (GC). GT was administered twice weekly by gavage with 100 µl of rifampicin resistant porcine L. plantarum LP5 at a concentration of 1010 CFU. Mice were fed water and food ad libitum throughout the experiment. Colonization in both groups was evaluated by the presence of clinical signs and by a microbiological analysis of the feces. Sampling was performed at weekly intervals to quantify the populations of the supplied L. plantarum LP5, Lactobacillus spp., Escherichia coli, total coliforms, Campylobacter and yeast. The results of the sampling in the GT in the week showed a recovery of L. plantarum LP5 above 5 logUFC/g, in the second it was 7 logUFC/g and in the third it was 5 logUFC/g. The CG, meanwhile, maintained the null count throughout the experiment. The results showed that there was no imbalance between the microbial components. Therefore, it is feasible to affirm that the inoculum did not alter the balance of the intestinal ecosystem. Particularly, it did not exert an inhibitory effect on the members of the previously established microbiota.

Donairs (Gyros) - Potential Hazards and Control

1986 ◽  
Vol 49 (5) ◽  
pp. 369-377 ◽  
Author(s):  
EWEN C. D. TODD ◽  
R. SZABO ◽  
F. SPIRING
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Heat Source ◽  
Bacillus Cereus ◽  
Clostridium Perfringens ◽  
Temperature Measurements ◽  
Microbiological Analysis ◽  
Short Period ◽  
And Control

Because of concerns that meat in donairs could allow growth of pathogens during cooking and overnight cooling of leftovers, 34 donairs from eleven establishments had temperatures taken and were examined microbiologically. Temperatures varied depending on depth of measurement and stage from the raw product to reheated leftovers. These were frequently >4 or <60°C and could be considered at temperatures favorable for growth of pathogens. Although aerobic colony counts were high (mean of 105 to 107 CFU/g), counts tended to decrease the longer the donair remained cooking on the spit. Staphylococcus aureus, Bacillus cereus, Clostridium perfringens and Escherichia coli were never more than 104/g despite some abusive practices, such as leaving donairs on the spit with the heat source turned off because the demand was low. Salmonella was found only in raw chicken slices to be used in donairs. It is recommended that good hygienic practices be encouraged at donair establishments and temperature measurements of donairs taken to verify these. Only if meat is <50°C at 1 cm below the surface during cooking or >5°C for the raw product or cooled leftovers, should samples be considered for microbiological analysis unless abusive practices have been observed. Because temperatures may vary over a short period of time during cooking, at least five measurements are recommended for each stage of the donair life (raw product, cooking donair, cooled leftovers and reheating donairs).

scholarly journals Histopathological Study for the Effect of Ethanolic Extract of Sonchus Oleraceus on Escherichia Coli Bacteria in Mice

2013 ◽  
Vol 37 (2) ◽  
pp. 167-174
Author(s):  
Mohammed J. Alwan
Keyword(s):  
Escherichia Coli ◽  
Ethanolic Extract ◽  
Inflammatory Cells ◽  
Inhibitory Effect ◽  
Histopathological Study ◽  
Internal Organs ◽  
Sonchus Oleraceus ◽  
The Third ◽  
Escherichia Coli Infection ◽  
Escherichia Coli Bacteria

    This study was designed to explore the effect of the ethanolic extract of Sonchus oleraceus on Escherichia coli infection in mice. The LD50 of the orally dosage ethanolic extract of Sonchus oleraceus was found to be 800gm/kg B.W for two weeks. Twenty five mice were randomly divided into five groups. The first group was given orally the ethanolic extract with dose 800 mg/kg B.W for two weeks, then infected with Escherichia coli bacteria with dose 1×108 cfu for ten days. Second group was infected with Escherichia coli bacteria with dose 1×108 cfu for ten days then treated with ethanolic extract of Sonchus oleraceus for two weeks. The third group was infected only with Escherichia coli bacteria with a dose 1×108 cfu for ten days. Fourth group was given orally the ethanolic extract with a dose 800 mg/kg B.W orally for two weeks. Fifth group was given normal saline solution and serived as control The histopathological study showed pathological lesions in most of internal organs of the third group that infected with Escherichia coli bacteria specially liver, kidney and intestine. First group revealed lesions less than the third group, while the second group showed just aggregation of inflammatory cells in some internal organs and this refers that the  ethanolic extract of Sonchus oleraceus  have obvious inhibitory effect on Escherichia coli bacteria when compared with the infected group.

scholarly journals Clinical signs in Japanese quails (Coturnix coturnix) experimentally infected with Salmonella Gallinarum

2016 ◽  
Vol 10 (3) ◽  
pp. 253 ◽  
Author(s):  
Roberta Cristina da Rocha e Silva ◽  
William Maciel Cardoso ◽  
Régis Siqueira de Castro Teixeira ◽  
Camila Muniz Cavalcante ◽  
Clarice Pessoa Almeida ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Ovarian Follicles ◽  
Control Group ◽  
Microbiological Analysis ◽  
Salmonella Gallinarum ◽  
Coturnix Coturnix ◽  
Fowl Typhoid ◽  
Japanese Female ◽  
And Control ◽  
Splenic Necrosis

Fowl typhoid a high-impact disease in the poultry industry, this study aims to verify the clinical signs of the disease in experimentally inoculated with quail Salmonella Gallinarum 54 Japanese female quails were distributed into two groups. The inoculated group (GI) SG (32 quail) and control group (CG) (16 birds). A total of six birds were euthanized before the start of the experiment, organs samples (liver, spleen, ovarian follicles, cecum and lung) were collected for the microbiological analysis. The birds of the GI group received 0,7mL of inoculum (1.5x106 CFU/mL) and birds of the CG group received 0,7 mL of saline solution. Thereafter, the quails were observed for ten consecutive days in three daily periods (8:00, 11:00 e 14:00) in order to observe clinical signs of disease. After four days of inoculation was verified prostration, apathy, ruffled feathers, diarrhea, bird's stay in the corner of the cage with closed eyes and reluctance to move (13/32). Some macroscopic changes were observed in euthanized birds with clinical signs like splenomegaly and splenic necrosis in 84,6% (11/13) and 23,0% (3/13) respectively, hepatomegaly and liver necrosis in 15,4% (2/13) e 23% (3/13) respectively, and atrophy and hemorrhage of ovarian follicles in 15,4% (2/13) and 7,7% (1/13) respectively. the birds that died was observed only hepatosplenomegaly. So we can conclude experimentally inoculated with Salmonella Gallinarum quail have the typical clinical signs of fowl typhoid observed in others birds of Galliforme order.

scholarly journals THERAPEUTIC PLASMAPHERESIS IN PRIMARY PRESENTATION OF DIABETES MELLITUS WITH DIABETIC KETOACIDOSIS, HYPERTRIGLYCERIDEMIA AND ACUTE PANCREATITIS

2018 ◽  
Vol 5 (3) ◽  
pp. 166-170
Author(s):  
Ana Maria Tonceanu ◽  
R. Palade ◽  
R. R. Grigorescu ◽  
T. Trotea ◽  
Florentina Mușat
Keyword(s):  
Diabetes Mellitus ◽  
Acute Pancreatitis ◽  
Clinical Signs ◽  
Multimodal Analgesia ◽  
The Third ◽  
Severe Hypertriglyceridemia ◽  
History Of ◽  
Essential Arterial Hypertension ◽  
And Control ◽  
Primary Presentation

We present a case of severe hypertriglyceridemia-induced acute pancreatitis associated withdiabetic ketoacidosis, managed in the intensive care unit. The patient was a 39-year-old woman, with amedical history of Graves-Basedow disease, essential arterial hypertension and morbid obesity(body mass index=31). Firstly, we aggressively corrected hypovolemia and hemodynamicimbalances (administrating fluids and systemic anticoagulant) and then we focused on the infectionprevention and control of intra-abodminal pressure, for the latter outcome. We decided to start the firstsession of plasma exchange. The patient responded well to the treatment applied. Plasma-exchangewas very efficient, reducing TG levels by 60% after the first treatment and achieving a decrease of77.6% at the third plasmapheresis session. This case was safely and effectively managed withplasmapheresis (three sessions), antibiotics, multimodal analgesia (intravenous and thoracicepidural catheter), early jejunal nutrition and forced mobilization. The patient's evolution wassignificantly favorable, with a reduction of the peripancreatic necrosis on computer scan, at day 28and she was discharged with a normal value of TG and without pain or any clinical signs.

THE 2019 NOVEL PANDEMIC OF CORONAVIRUS DISEASE (COVID-19): A STUDY OF EXISTING EVIDENCE IN THE INDIAN POPULATION

2020 ◽  
Vol 7 (2) ◽  
pp. 199-204
Author(s):  
Shrikant Verma ◽  
Mohammad Abbas ◽  
Sushma Verma ◽  
Syed Tasleem Raza ◽  
Farzana Mahdi
Keyword(s):  
Disease Transmission ◽  
Indian Population ◽  
Respiratory Infections ◽  
Clinical Signs ◽  
Case Fatality ◽  
Public Health Emergency ◽  
The World ◽  
And Control ◽  
General Wellbeing ◽  
Different Parts

A novel spillover coronavirus (nCoV), with its epicenter in Wuhan, China's People's Republic, has emerged as an international public health emergency. This began as an outbreak in December 2019, and till November eighth, 2020, there have been 8.5 million affirmed instances of novel Covid disease2019 (COVID-19) in India, with 1,26,611 deaths, resulting in an overall case fatality rate of 1.48 percent. Coronavirus clinical signs are fundamentally the same as those of other respiratory infections. In different parts of the world, the quantity of research center affirmed cases and related passings are rising consistently. The COVID- 19 is an arising pandemic-responsible viral infection. Coronavirus has influenced huge parts of the total populace, which has prompted a global general wellbeing crisis, setting all health associations on high attentive. This review sums up the overall landmass, virology, pathogenesis, the study of disease transmission, clinical introduction, determination, treatment, and control of COVID-19 with the reference to India.

THE EFFECT OF APPLYING DEMONSTRATION METHOD ON STUDENTS’ ACHIEVEMENT IN SPEAKING SKILL AT THE THIRD GRADE OF SMK PAB 3 MEDAN ESTATE

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Meryanti Napitupulu And Anni Holila Pulungan
Keyword(s):  
High School ◽  
Control Group ◽  
Vocational High School ◽  
Control Groups ◽  
Speaking Test ◽  
The Third ◽  
Speaking Skill ◽  
The Subject ◽  
And Control ◽  
Experimental Group

This study was conducted as an attempt to discover the effect of applying Demonstration Method on students’ achievement in speaking skill. It was an experimental research. The subject was students of Grade XII, Vocational High School (Sekolah Menengah Kejuruan: SMK), which consisted of 79 students. The research was divided into two groups: experimental and control groups. The instrument used to collect the data was speaking test. To obtain the reliability of the test, the writer applied Kuder Richardson 21 formula. The result of the reliability was 0.7, and it was found that the test was reliable. The data were analyzed by using t-test formula. The analysis showed that the scores of the students in the experimental group were significantly higher than the scores of the students in the control group at the level of significant m = 0.05 with the degree of freedom (df) 77, t-observed value 8.9 > t-table value 1.99. The findings indicate that using Demonstration Method significantly affected the students’ achievement in speaking skill. So, English teachers are suggested to use Demonstration Method in order to improve students’ achievement in speaking skill.

Study effect of crude extracts of Hibiscus sabdarifa L. on some Enterobacteriaceae

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sabreen A Kamal ◽  
Ishraq A Salih ◽  
Hawraa Jawad Kadhim ◽  
Zainab A Tolaifeh
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Bacterial Growth ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Crude Extracts ◽  
And Control

Red rose or roselle (beauty rose ) is natively known as red tea belong to Malvaceae, it is flowers use traditionally for antihypertensive hepato protective, anticancer,antidiabetic,antibacterial, cytotoxicity and antidiarreal, By preparing red tea from it's flower. In this study, we extract chemical compounds by using two solvent which are Ethanol, Ethyl acetate. so we can extract Anthocyanin which is responsible for red colour of flower with many chemical compounds. then study the effect of these extracts on 5 genera from Enterobacteriacaea which can cause diarrheae (Shigella, Salmonella, Escherichia coli, Proteus and Klebsiella ) by preparing 3 concentrations for each solvent (250, 500, 750 ) mg/ml, and control then compare with two antibiotic (Azereonam 30 mg/ml and Bacitracin 10 mg/ml ) these extracts revealed obvious inhibition zone in bacterial growth.

MODEL OF SOCIAL POTENTIAL OF CHILDHOOD IN THE RUSSIAN REGIONS: THE RESULTS OF EXPERIMENTS

2019 ◽  
pp. 152-162
Author(s):  
A.G. Filipova ◽  
A.V. Vysotskaya
Keyword(s):  
Third Wave ◽  
Russian Regions ◽  
The Third ◽  
Control Factors ◽  
Social Potential ◽  
System Input ◽  
Optimal Values ◽  
Fixed Ideal ◽  
And Control ◽  
Selection Of

The article presents the results of mathematical experiments with the system «Social potential of childhood in the Russian regions». In the structure of system divided into three subsystems – the «Reproduction of children in the region», «Children’s health» and «Education of children», for each defined its target factor (output parameter). The groups of infrastructure factors (education, health, culture and sport, transport), socio-economic, territorial-settlement, demographic and en-vironmental factors are designated as the factors that control the system (input parameters). The aim of the study is to build a model îf «Social potential of childhood in the Russian regions», as well as to conduct experiments to find the optimal ratio of the values of target and control factors. Three waves of experiments were conducted. The first wave is related to the analysis of the dynam-ics of indicators for 6 years. The second – with the selection of optimal values of control factors at fixed ideal values of target factors. The third wave allowed us to calculate the values of the target factors based on the selected optimal values of the control factors of the previous wave.

scholarly journals Stage-dependent effect of deferoxamine on growth of Plasmodium falciparum in vitro

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1250-1255 ◽  
Author(s):  
S Whitehead ◽  
TE Peto
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Antimalarial Activity ◽  
Clinical Malaria ◽  
Inhibitory Effect ◽  
Parasite Development ◽  
And Control ◽  
Speed Of Onset

Abstract Deferoxamine (DF) has antimalarial activity that can be demonstrated in vitro and in vivo. This study is designed to examine the speed of onset and stage dependency of growth inhibition by DF and to determine whether its antimalarial activity is cytostatic or cytocidal. Growth inhibition was assessed by suppression of hypoxanthine incorporation and differences in morphologic appearance between treated and control parasites. Using synchronized in vitro cultures of Plasmodium falciparum, growth inhibition by DF was detected within a single parasite cycle. Ring and nonpigmented trophozoite stages were sensitive to the inhibitory effect of DF but cytostatic antimalarial activity was suggested by evidence of parasite recovery in later cycles. However, profound growth inhibition, with no evidence of subsequent recovery, occurred when pigmented trophozoites and early schizonts were exposed to DF. At this stage in parasite development, the activity of DF was cytocidal and furthermore, the critical period of exposure may be as short as 6 hours. These observations suggest that iron chelators may have a role in the treatment of clinical malaria.

scholarly journals Mating Disruption of Pseudococcus calceolariae (Maskell) (Hemiptera, Pseudococcidae) in Fruit Crops

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 343
Author(s):  
Carolina Ballesteros ◽  
Alda Romero ◽  
María Colomba Castro ◽  
Sofía Miranda ◽  
Jan Bergmann ◽  
...  
Keyword(s):  
Visual Inspection ◽  
Mating Disruption ◽  
Economic Importance ◽  
Fruit Crops ◽  
Potential Control ◽  
The Third ◽  
Pseudococcus Calceolariae ◽  
And Control ◽  
Potential Use ◽  
Control Tool

Pseudococcus calceolariae, the citrophilous mealybug, is a species of economic importance. Mating disruption (MD) is a potential control tool. During 2017–2020, trials were conducted to evaluate the potential of P. calceolariae MD in an apple and a tangerine orchard. Two pheromone doses, 6.32 g/ha (2017–2018) and 9.45 g/ha (2019–2020), were tested. The intermediate season (2018–2019) was evaluated without pheromone renewal to study the persistence of the pheromone effect. Male captures in pheromone traps, mealybug population/plant, percentage of infested fruit at harvest and mating disruption index (MDI) were recorded regularly. In both orchards, in the first season, male captures were significantly lower in MD plots compared to control plots, with an MDI > 94% in the first month after pheromone deployment. During the second season, significantly lower male captures in MD plots were still observed, with an average MDI of 80%. At the third season, male captures were again significant lower in MD than control plots shortly after pheromone applications. In both orchards, population by visual inspection and infested fruits were very low, without differences between MD and control plots. These results show the potential use of mating disruption for the control of P. calceolariae.

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