scholarly journals Trigona sp. Propolis Ethanolic Extract Decreased Chloramphenicol-induced Serum Glutamic Oxaloacetic Transaminase and Alkaline Phosphatase Levels of Rats (Rattus novergicus)

2018 ◽  
Vol 9 (3) ◽  
pp. 110
Author(s):  
Chanif Mahdi ◽  
Anna Zukiaturrahmah ◽  
Dyah Ayu Oktavianie Ardhiana Pratama ◽  
Putranty Widha Nugraheni
Keyword(s):  
Alkaline Phosphatase ◽  
Free Radicals ◽  
Liver Damage ◽  
Therapy Group ◽  
Ethanolic Extract ◽  
The Body ◽  
Male Rats ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Propolis Extract

Liver has an important role in detoxification of toxins such as xenobiotic which could interfere the function of liver. Chloramphenicol is an antibiotic widely used,despite of its toxicity potentials. The enhancement of free radicals in the body could suppress antioxidant activity. Propolis of Trigona sp. has been known to contain very high amount of antioxidants. The enhanced serum glutamic oxaloacetic transaminase (SGOT) and alkaline phosphatase (ALP) levels in serum is used as marker of liver damage due to the increase of free radicals. The purpose of this study was to observe the effect of Trigona sp. propolis ethanolic extract on SGOT and ALP levels in rats (Rattus novergicus) pretreated by chloramphenicol to induce liver damage. Test animals used for this research were male rats aged 8-12 weeks divided into five treatment groups: negative controlgroup (normal), positive control group (induced by 400 mg/kgBW chloramphenicol), first therapy group, second therapy group, third therapy group induced by chloramphenicol with and propolis extract with the dose of 8 mg, 16 mg, and 24 mg, respectively. Chloramphenicol was injected subcutaneously for 14 days, whereas propolis extract were administered orally for 21 days. The level of SGOT and ALP was determined using spectophotometry. The results showed that propolis extract could reduce levels of SGOT and ALP. Dose of 24 mg/kg was the effective dose to decrease levels of SGOT and ALP significantly (p<0.01). Hence, it may be concluded that the ethanol extract of propolis could be used as herbal therapy in rats model of liver damage.Keywords : ALP, liver, chloramphenicol, propolis, SGOT

scholarly journals Al-Qur�an dan Sains?(Bukti Kebenaran dalam Kasus Propolis Madu)

2014 ◽  
Vol 12 (1) ◽  
Author(s):  
MF. Zenrif Dan Erna Susanti
Keyword(s):  
Free Radicals ◽  
Liver Damage ◽  
Rhodamine B ◽  
Therapy Group ◽  
Carcinogenic Effect ◽  
Treatment Groups ◽  
Group 3 ◽  
Group 2 ◽  
Dose Levels ◽  
Rhodamin B

Exposure free radicals in the activity of this life can not be avoided, included exposure free radicals from food additive espesifically Rhodamine B. Carcinogenic effect of Rhodamin B can be caused by Poly Aromatic Hydrocarbons structure that are destructive to the liver as metabolism organ. The purpose of this study was to prove the antioxidant activity of Honey Propolis against liver damage caused by exposure of Rhodamine B as free radical sources. Indicator of liver damage can be indicated by MDA, a marker of oxidative stress that occurs in these organs. The methode was used experimental methode, with seven treatment groups, namely: (1) normal group, (2) the induction of Rhodamin B without propolis therapy group, (3) the induction of Rhodamin B with Honey Propolis therapy with three dose levels are 350 mg, 700 mg and 1050 mg that are given at intervals of two hours after the administration of Rhodamine B (4) Honey Propolis therapy groups are given at interval of 1 week aftertheadministrationof RhodamineBwiththreedoselevels.MDAlevelsweremeasuredbyTBARS method. The results showed that the levels of Malondialdehyde declined steadily in the group treated with honey propolis to less than control groups. There are significant differences in MDA levels between the treatment groups Honey Propolis therapy (p = 0.034, p <0.05). Based on these results, it can be concluded that Honey propolis dose 700 mg act as therapeutic agent in liver damage due to exposure of Rhodamine B significantly.The results ofthis studyprove thetruth ofthe Quraninthe QS. Al-Nahl (16): 69of thewonders ofhoneyin preventingliver damagedue toexposure tohazardous substancesin food.

scholarly journals Secondary hemophagocytic syndrome in adult patients. Study of 91 patients

2020 ◽  
Vol 15 (4) ◽  
pp. 52-64
Author(s):  
V. G. Potapenko ◽  
A. V. Klimovich ◽  
M. Yu. Pervakova ◽  
S. V. Lapin ◽  
O. V. Goloshchapov ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Unknown Origin ◽  
Multiple Organ ◽  
Glutamic Pyruvic Transaminase ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Allogenic Bone ◽  
Laboratory Changes

Background. Secondary hemophagocytic lymphohystiocytosis (sHLH) is a hyperinflammatory reaction provoked by some trigger (cancer, autoimmune or infection). The majority of affected patients are at high risk of fatal multiple organ failure without getting immunsupressive treatment.Objective. Clinical and laboratory profile of sHLH patients.Materials and methods. Retrospective study included clinical, instrumental and lab data from the 91 patients followed between June 2009 and June 2019. Diagnosis sHLH had been based on HLH-2004 and H-Score criteria. The analyzed parameters had been fever chart, liver and spleen enlargement, changes in the bone marrow; values levels of glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, alkaline phosphatase, bilirubin, triglycerides, total ferritin with percentage of glycosylation. All patients with rheumatic disorders or malignancies had received either immunosuppressive or cytotoxic therapy. Febrile patients received anti-infective treatment according to the local routine protocols.Results. The data from 91 patients (41 male and 50 female) had been analyzed. Median age was 58 (2–90) years. The sHLH trigger-diseases spectrum included leukemia/lymphoma (n = 52), infection diseases (n = 11), autoimmune disorders (n = 5), allogenic bone marrow transplantation (n = 13), unidentified (n = 10). A fever with an unknown origin and refractory to antibacterial treatment had been observed in 87 (96 %) patients. Morphological hemophagocytic evidences in the bone marrow had been found in 83 %. Breath shortening, liver failure, neurologic disturbances, systemic effusions, rash, heart failure had been registered in 83 % patients. Detected splenomegaly presented in 56 %. Laboratory changes, median were as following: serum glutamic-pyruvic transaminase (alanine aminotransferase, SGPT) – 92 (39.2–1060.8) IU/L; serum glutamic oxaloacetic transaminase (aspartate aminotransferase, SGOT) – 105 (40–4177) IU/L; alkaline phosphatase – 225 (120.9–989) IU/L; bilirubin – 50.5 (22–559) µmol/L; triglycerides – 3.2 (1.95–8.6) mmol/L; total ferritin – 10000 (597–255000) ng/mL with glycosylation percentage – 20.45 (0–37.8) %. 71 patients received various of HLH-directed therapy courses. The overall survival rate was 27 %, median follow-up – 540 days.Conclusion. The main clinical and instrumental findings in sHLH are fever, refractory to anti-infective treatment, elevation of transaminases, serum alkaline phosphatase, triglycerides, total ferritine with low glycosylated fraction. Early diagnosing and immunesupression are the main factors of survival.

scholarly journals EFFECT OF ETHANOLIC EXTRACT OF TINOSPORA CRISPA L FROM INDONESIA IN ALLOXAN INDUCED LIVER DAMAGE

2017 ◽  
Vol 9 (8) ◽  
pp. 65
Author(s):  
Emsutrisna . ◽  
Fahrizal Aria Sahadewa ◽  
Ikbar Ardiansyah
Keyword(s):  
Body Weight ◽  
Liver Damage ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Hepatoprotective Effect ◽  
Male Rats ◽  
Histopathologic Examination ◽  
Wistar Strain ◽  
Alloxan Monohydrate ◽  
Group 1

Objective: The objective of this study was to evaluate the hepatoprotective effect of Tinospora crispa L. (Bratawali).Methods: Twenty four male rats wistar strain were divided into four groups. The serum alanine amino transferase (ALT) and aspartate aminotransferase (AST) of rats were measured (day 0). Rats then were injected by alloxan monohydrate at doses of 120 mg/200 bw (g) intraperitoneally. Four days later, the serum ALT and AST of rats were measured (second measurement/day 4) and then were treated by extract appropriate their groups. Group 1 was treated by 2 ml of distilled water orally; group 2,3 and 4 were treated by 70% ethanolic extract of T. crispa L. (EETC) at dose of 100; 200 and 400 mg/200 bw (g)/day respectively orally. After 10 d treatment, serum ALT and AST were measured (third measurement/day 14). At the end of this treatment, all rats were killed for histopathologic examination of their liver. The histopathologic examination was performed to assess the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei.Results: The result of this study showed that the ethanolic extract of T. crispa L at dose of 100 and 200 mg/200gbw can reduce blood ALT and AST significantly (P<0.05). From the histopathological examination, it was found that the number of pyknotic nuclei, karyorrhexis nuclei and karyolysis nuclei of EETC at doses of 100 and 200 mg/200 body weight (g) lower than negative control.Conclusion: The present study shows that the 70% EETC at dose of 100 and 200 mg/200 body weight (g) has hepatoprotective effect against alloxan induced liver damage.  

Hepatoprotective and antioxidant effect of Adiantum lunulatum Burm. F. leaf in alcohol-induced rat model

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Nimisha Parshottambhai Kakadia ◽  
Monika A Amin ◽  
Shrikalp S Deshpande
Keyword(s):  
Total Bilirubin ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Antioxidant Effect ◽  
Serum Enzymes ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Pyruvic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Experimental Animals

ABSTRACTBackgroundAdiantum lunulatum Burm. F. leaf (AL) and its related species have been used traditionally for the treatment of various diseases.ObjectiveThe present study evaluated the hepatoprotective, and antioxidant activities of ethanolic extract of AL.Methodology and ResultThe hepatoprotective effect of AL was evaluated against ethanol-induced hepatotoxicity in rats. Administration of ethanol (2 g/kg) showed a significant biochemical and histological deterioration in the liver of experimental animals. Pretreatment with ethanolic extract of AL (250 and 500 mg/kg b.wt. p.o) significantly reduced the elevated levels of serum enzymes like serum glutamic-oxaloacetic transaminase (AST), serum glutamic-pyruvic transaminase (ALT), alkaline phosphatase (ALP), total protein, total bilirubin and reversed the hepatic damage in the liver which evidenced the hepatoprotective activity. The superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) level notably increased due to doses of AL.ConclusionThe results of the present study demonstrate that the ethanolic extract of AL possesses hepatoprotective and antioxidant activities.Graphical Abstract

scholarly journals The Increase Activity of Lipoprotein Lipase (LPL) Enzyme and Histophatological Changes of Liver of Hypercholesterolemic Rat (Rattus norvegicus) Induced by Ethanolic Extract of Ant Plant (Myrmecodia sp.)

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Roslizawaty R ◽  
Rusli R ◽  
Nazaruddin N ◽  
Syafruddin S ◽  
Indahlia Syahfitri Bangun ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Lipoprotein Lipase ◽  
Liver Damage ◽  
Fatty Infiltration ◽  
Ethanolic Extract ◽  
Fatty Degeneration ◽  
Negative Control Group ◽  
Negative Control ◽  
Male Rats ◽  
Control Group

This study was aimed to find out the effect of ethanolic extract of ant plant (Myrmecodia sp.) to increase the activity of enzyme lipoprotein lipase (LPL) serum and to observe the histopathological changes of hypercholesterolemic rat liver. This study used 20 male rats grouped into 4treatment groups, namely negative control group (K1), hypercholesterolemic group (K2), and hypercholesterolemic group that administered withethanolic extract of ant plant 100 (K3) and 200 mg/kg bw (K4). The LPL enzyme activity were measured by the titration method andhistopatological changes of liver were observed by calculated fatty degeneration and fatty infiltration. The data were analyzed using one wayanova followed by Duncan test. The average of LPL enzyme activity on group K1, K2, K3, and K4 were 0.80±0.06, 0.45±0.10, 0.83±0.11, and0.76±0.03 unit, respectively. The average number of fatty degeneration on hepatocyte and fatty infiltration were 1.80±0.83, 3.60±1.14, 23.00±1.22, and 40.20±1.30; and 9.20±0.84, 16.40±1.14, 2.60±0.54, and 4.80±0.83, respectively. The results showed that theraphy ethanolic extract ofant plant effects significantly (P<0.01) on the increase of enzyme LPL and improve liver damage in hypercholesterolemic male rats. To concludethe administration of ethanolic extract of ant plant increases the LPL enzyme activity and improves liver damage on hyperch olesterolemic rats.Key words: Myrmecodia sp., LPL activity, histopathological liver, hypercholesterolemia

scholarly journals Hepatoprotector Effect of Coconut Water (Cocos nucifera L.) and Folic Acid to the Liver Histopathological Desccription of Pregnant Wistar Female Rats (Rattus norvegicus) Induced by Carbamate

2020 ◽  
Vol 6 (1) ◽  
pp. 53
Author(s):  
Muhammad Rosyid Ridho ◽  
Aris Prasetyo ◽  
Hairrudin Hairrudin
Keyword(s):  
Free Radicals ◽  
Folic Acid ◽  
Liver Damage ◽  
Liver Cells ◽  
The Body ◽  
Coconut Water ◽  
Female Rats ◽  
Test Results ◽  
Liver Histopathology ◽  
Significant Difference

Abstract Carbamate is an insecticide with a working mechanism that inhibits the enzyme acetylcholineestrase (AChE). Obstacles to AChE will cause the formation of excessive free radicals in the body causing oxidative stress and causing lipid peroxidation in body cells, including hepatocyte cells in the liver. Pregnant women have a change in detoxification activity in the liver due to exposure to xenobiotic substances during pregnancy causing a decrease in cytochrome P450 1A2 (CYP1A2) which will cause an increase in free radicals and can damage liver cells. Coconut water contains antioxidants that can neutralize free radicals in the body. While folic acid can encourage improvement in morphology of liver cells. The purpose of this study was to determine the hepatoprotector effect of coconut water and folic acid on the histopathology of the liver of pregnant female wistar rats induced by carbamate. The design of this study is true experimental with a post test only control group design. The sampling method used is simple random sampling. The number of samples in this study were 28 rats divided into 4 groups K (aquades), P1 (carbamate), P2 (carbamate and coconut water), and P3 (carbamate and folic acid). At the end of the study rat liver was taken to then become histological preparations. The One Way ANOVA test results showed a significant difference between groups compared (p <0.05). Post hoc LSD test results showed that group P1 had damaged liver histopathology, there were significant differences with group K and P3 (p <0.05), but there were no significant differences when compared with group P2 (p = 0.826). Meanwhile, the P3 group had significant differences when compared with all groups (p <0.05). The conclusion of this study is that giving coconut water cannot prevent liver damage due to carbamate induction, while folic acid has been shown to prevent liver damage due to carbamate induction, from liver histopathology. Keywords: carbamate, coconut water, folic acid, liver histopathology

scholarly journals Вплив вуглецевих наночастинок на гепатотоксичність тетрахлорметану

2018 ◽  
Author(s):  
N. Ya. Letniak ◽  
M. M. Korda
Keyword(s):  
Carbon Nanotubes ◽  
Alkaline Phosphatase ◽  
Thermal Properties ◽  
Carbon Nanoparticles ◽  
Soft Tissues ◽  
The Body ◽  
Male Rats ◽  
Multi Walled Carbon Nanotubes ◽  
A Cell ◽  
Walled Carbon Nanotubes

Introduction. Carbon nanoparticles have unique mechanical, electrical and thermal properties and are widely used in scientific research, industry and medicine. They are a promising nanomaterial for medical use due to their really high biocompatibility with blood, bones, cartilages and soft tissues. The capability of nanotubes to transport medicines and chemicals inside a cell predicts the possibility of the increase of classical substances toxicity in case of their intake into the body together with nanotubes.The aim of the study – to determine how nanoparticles affect the hepatotoxic properties of  tetrachloromethane.Research Methods. The experiments were performed on outbred male rats, which were administered intraperitoneally with 0.5 ml of suspension of single-walled, multi-walled or multi-walled functionalized by COOH nanotubes (60 mg/kg) separately or together with tetrachlormethane (2 ml/kg). The animals were taken out of the experiment in 3, 6 and 48 hours after the administration of the nanotubes and tetrachlormethane. Alanine and aspartate transaminases, alkaline phosphatase activities, as well as total protein and urea contents were measured in rat blood plasma.Results and Discussion. It has been shown that only multi-walled carbon nanotubes changed significantly  the studied parameters. The administration of tetrachloromethane to rats caused significant changes of all indices.Maximal changes of all parameters were registered in the group of animals that were coadministered with carbon nanotubes and tetrachloromethane. In this case, activities of transaminases and alkaline phosphatase in blood significantly changed compared to the similar indicators in the group of animals, which were administered with the chemical toxicant only.Conclusion. Carbon nanotubes enhance the negative hepatotoxic effects of tetrachlormethane.

Elucidation of Malaria on Metabolic Activities During Pregnancy

2019 ◽  
Vol 15 (3) ◽  
pp. 218-222
Author(s):  
Saira Baloch ◽  
Xiaofang Pei ◽  
Jiayi Chen ◽  
Yuhang Chen ◽  
Shafi Muhammad ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Pregnant Women ◽  
Aspartate Aminotransferase ◽  
Vivax Malaria ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Iron Copper ◽  
Metabolic Activities

Background:Malaria is extended to more than 90 countries of the world, and is also considered as a major public health and socio-economical problem.Objective:The aim of this study is the elucidation of malaria on metabolic activities in pregnancy.Material and Methods:A total of 60 patients with gestational vivax malaria were recruited in this study, and 60 healthy pregnant women were selected as a control group. These women were selected using simple random sampling technique. A sample of 5 ml venous blood was drawn from each patient, centrifuged at 5000 rpm for 20 minutes to separate the serum. The serum samples were analyzed for enzyme activity such as Alkaline Phosphatase (ALP), Creatine Phospho Kinase (CPK), Lactate Dehydrogenase (LDH), and Serum Glutamic Oxaloacetic Transaminase / aspartate aminotransferase (SGOT/AST) by using kit method on Microlab 300 and trace metal content Magnesium, Iron, Copper and Zinc (Mg, Fe, Cu, and Zn) by Atomic Absorption Spectroscopy (Varian AAS Model A-20).Results:There was an increased level of Alkaline Phosphatase (ALP), Creatine Phospho Kinase (CPK), Lactate Dehydrogenase (LDH), and Serum Glutamic Oxaloacetic Transaminase / aspartate aminotransferase in sixty pregnant women suffering from vivax malaria as compared to the control subjects. Whereas, the low concentrations of serum iron, copper, magnesium and zinc were observed in patients as compared to the control subjects.Conclusion:The findings of this study suggest that in order to enhance the immunity and develop resistance towards malaria during pregnancy, it is necessary to balance enzyme activity and provide diverse metals in the therapy to cure this disease.

scholarly journals Propolis Prevents Hepatorenal Injury Induced by Chronic Exposure to Carbon Tetrachloride

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Monika Bhadauria
Keyword(s):  
Oxidative Stress ◽  
Alkaline Phosphatase ◽  
Carbon Tetrachloride ◽  
Ethanolic Extract ◽  
Protective Role ◽  
Sprague Dawley ◽  
Chronic Injury ◽  
Biochemical Alterations ◽  
Propolis Extract ◽  
Liver And Kidney

Carbon tetrachloride (CCl4) is a well-known hepatotoxicant, and its exposure induces hepatorenal injury via oxidative stress and biochemical alterations. This study had been conducted to confirm the protective role of propolis extract on CCl4-induced hepatorenal oxidative stress and resultant injury. Propolis extracts collected from Gwalior district and 24 female Sprague Dawley rats were used for experiment. Animals were exposed to CCl4(0.15 mL/kg, i.p.) for 12 weeks (5 days/week) followed by treatment with propolis extract (200 mg/kg, p.o.) for consecutive 2 weeks. CCl4exposure significantly depleted blood sugar and hemoglobin level and raised the level of transaminases, alkaline phosphatase, lactate dehydrogenase, protein, urea, albumin, bilirubin, creatinine, triglycerides, and cholesterol in serum. Lipid peroxidation was enhanced, whereas GSH was decreased significantly in liver and kidney in CCl4-intoxicated group. Ethanolic extract of propolis successfully prevented these alterations in experimental animals. Activities of catalase, adenosine triphosphatase, glucose-6-phosphatase, acid, and alkaline phosphatase were also maintained towards normal with propolis therapy. Light microscopical studies showed considerable protection in liver and kidney with propolis treatment, thus, substantiated biochemical observations. This study confirmed hepatoprotective potential of propolis extract against chronic injury induced by CCl4by regulating antioxidative defense activities.

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