scholarly journals Total Hyptolide of Indonesian Hyptis pectinata extracts in a various solvent using HPLC and UV-Vis spectroscopy and their toxicities

2019 ◽  
Vol 22 (6) ◽  
pp. 305-309
Author(s):  
Meiny Suzery ◽  
Siti Khumairoh ◽  
Bambang Cahyono
Keyword(s):  
Quantitative Analysis ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Uv Spectroscopy ◽  
Bioactive Compound ◽  
Dry Weight ◽  
Pure Compound ◽  
Standard Curve ◽  
Lc50 Value ◽  
Vis Spectroscopy

The bioactive compound of hyptolide has been isolated from an Indonesian plant Hyptis pectinata using various solvents. The pure compound was then used as a standard in quantitative analysis using HPLC and UV-Vis spectroscopy in ethanol (EEth), ethyl acetate (EEa), acetone (Eac) and hexane (Ehex) extract each earned 3.14%, 0.01%, 1.33% and 0.04% (dry weight per sample). The standard curve of hyptolide using UV spectroscopy has been obtained with the coefficient of relationship (r) of 0.997. However, the use of spectroscopy was not recommended for the standardization of hyptolide in the extract due to interference from other compounds that absorb the same wavelength. Furthermore, the toxicity test using the Brine Shrimp lethality test shows LC50 value of 92-181 ppm, which was in EEa compared to EEth, Eac, and Ehex.

scholarly journals Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract

Elkawnie ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 67
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca ◽  
Fajar Indah Puspita Sari
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Positive Control ◽  
Acetone Extract ◽  
Antidiabetic Activity ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Ft Ir

Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.

scholarly journals TOXICITY TEST OF EXTRACTS OF THE FOUR SEA CUCUMBER (FAMILY HOLOTHURIIDAE) FROM EAST PENJALIRAN ISLAND, SERIBU ISLANDS, JAKARTA BASED ON THE BRINE SHRIMP LETHALITY TEST (BSLT)

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Arum Albuntana ◽  
Yasman Yasman ◽  
Wisnu Wardhana
Keyword(s):  
Crude Extract ◽  
Brine Shrimp ◽  
National Park ◽  
Sea Cucumber ◽  
Bioactive Compound ◽  
Active Species ◽  
Brine Shrimp Lethality ◽  
Water Fraction ◽  
Lc50 Value ◽  
Toxicity Level

<p>Sea cucumber is one of the marine bioactive compounds resources . The properties of the bioactive compound are biologically shown by their functions as anticancer, antifungi, hemolytic, and immunomodulatoryt. The purpose of this research is to predict (determine) the toxicity level of the extracts of Actinopyga miliaris, Holothuria leucospilota, Bohadschia argus, and Bohadschia marmorata collected from Penjaliran Timur Island, Seribu Island National Park Jakarta. The method used in this research is Brine Shrimp Lethality Test (BSLT). The result of this research showed that all of the extract from those species are active in BSLT, indicated by LC50 values of less than 1000 μg/ml. Bohadcshia argus is highest active species indicated by LC50 value 69,254 μg/ml. Water fraction of H. leucospilota’s crude extract is the most active fraction indicated by LC50 50,968 μg/ml.</p><p>Keywords: Brine Shrimp Lethality Test, Crude Extract, Fractionation, Family Holothuriidae, Sea Cucumber</p>

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals TOXICITY TEST OF EXTRACTS OF THE FOUR SEA CUCUMBER (FAMILY HOLOTHURIIDAE) FROM EAST PENJALIRAN ISLAND, SERIBU ISLANDS, JAKARTA BASED ON THE BRINE SHRIMP LETHALITY TEST (BSLT)

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Arum Albuntana ◽  
Yasman Yasman ◽  
Wisnu Wardhana
Keyword(s):  
Crude Extract ◽  
Brine Shrimp ◽  
National Park ◽  
Sea Cucumber ◽  
Bioactive Compound ◽  
Active Species ◽  
Brine Shrimp Lethality ◽  
Water Fraction ◽  
Lc50 Value ◽  
Toxicity Level

Sea cucumber is one of the marine bioactive compounds resources . The properties of the bioactive compound are biologically shown by their functions as anticancer, antifungi, hemolytic, and immunomodulatoryt. The purpose of this research is to predict (determine) the toxicity level of the extracts of Actinopyga miliaris, Holothuria leucospilota, Bohadschia argus, and Bohadschia marmorata collected from Penjaliran Timur Island, Seribu Island National Park Jakarta. The method used in this research is Brine Shrimp Lethality Test (BSLT). The result of this research showed that all of the extract from those species are active in BSLT, indicated by LC50 values of less than 1000 μg/ml. Bohadcshia argus is highest active species indicated by LC50 value 69,254 μg/ml. Water fraction of H. leucospilota’s crude extract is the most active fraction indicated by LC50 50,968 μg/ml.Keywords: Brine Shrimp Lethality Test, Crude Extract, Fractionation, Family Holothuriidae, Sea Cucumber

scholarly journals Uji Toksisitas Ekstrak Batang Pinang Yaki (Areca vestiaria) pada Artemia salina Leach.

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Windy Astuti Tampungan
Keyword(s):  
Medicinal Plant ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Toxicity Level ◽  
Supernatant Solution

AbstrakEfek toksisitas pinang yaki (Areca vestaria) sebagai tumbuhan obat potensial perlu diuji untuk mengetahui ambang batas penggunaannya. Penelitian ini bertujuan untuk menguji tingkat toksisitas ekstrak batang pinang yaki terhadap larva Artemia salina Leach. dengan metode Brine Shrimp Lethality Test. Ekstrak diencerkan dengan konsentrasi 200, 400, 600 dan 800 ppm masing-masing untuk supernatan rendaman pertama (larutan A),supernatan rendaman ke dua (larutan B) dan supernatan rendaman ke tiga (larutan C) . Hasil penelitian menunjukkan bahwa ekstrak batang pinang yaki mempunyai efek toksisitas dengan nilai LC50 sebesar 398,28 ppm untuk larutan A, 390,84 ppm untuk larutan B dan 438,53 ppm untuk larutan C.Kata kunci: batang Areca vestaria,LC50, uji toksisitasAbstractToxicity effect of pinang yaki (Areca vestiaria) as potential medicinal plant should be evaluated to know its application threshold. This research aimed to evaluate the toxicity level of pinang yaki trunk extract on larve of Artemia salina Leach. using the method of Brine Shrimp Lethality Test. Extract was diluted to concentration of 200, 400, 600 and 800 ppm each for solution A (first soaking supernatant), solution B (second soaking supernatant)and solution C (third soaking supernatant). The result of this research showed that extract of pinang yaki trunk had a toxic characteristic, with LC50 value was 398,28, 390,84, and 438,53 ppm respectively for solution A, B and C.Keywords: LC50 , toxicity test, trunk of Areca vestaria

scholarly journals Isolasi dan Identifikasi Steroid dari Ekstrak N-Heksana Daun Afrika (Vernonia amygdalina Del.)

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 18-22
Author(s):  
Evi Mashunah ◽  
Erwin ◽  
Saibun Sitorus
Keyword(s):  
Data Analysis ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Test Results ◽  
Separation And Purification ◽  
Vernonia Amygdalina ◽  
Isolation And Identification ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ft Ir

Isolation and identification of steroids from the n-hexane fraction of Vernonia amygdalina Del. was carried out. Separation and purification were performed using flash chromatographic and gravity chromatography. Based on the results of phytochemical tests supported by UV and FT-IR data analysis, the isolate obtained was a steroid (sterol type). Toxicity test results using the Brine Shrimp Lethality Test (BSLT) were known that the steroid has moderate toxicity with an LC50 value of 48.39 ppm. Keywords: Vernonia amygdalina Del., Toxicity, BSLT, Sterol, Steroid

scholarly journals Studies on Antifungal Activity and Brine Shrimp Toxicity Argemone mexicana

KYAMC Journal ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 402-405
Author(s):  
Ainun Nahar ◽  
Anwar Habib ◽  
MA Awal ◽  
AKM Shahidur Rahman ◽  
MA Gafur Mondal ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Biologically Active ◽  
Good Activity ◽  
Argemone Mexicana ◽  
Lc50 Value ◽  
Brine Shrimp Toxicity

The present study determined the antifungal activity of the root of extracts Argemone mexicana against 7 fungi using dichloromethane and methanol as solvent. Among these pathogens, 3 were dermatophytes and the rest 4 were nondermatophytes. The dichloromethane extract of Argemone mexicana exhibited moderate to good activity at concentration of 200 ?g/disc but at concentration of 50 ?g/disc, the same extract showed no activity against the tested fungi. The methanol extract were also inactive against the tested pathogens. In brine shrimp toxicity test, it was observed that LC50 value of the dichloromethane extract of Argemone mexicana was 22.35 ?g/ml. From these findings it is indicative that dichloromethane extract of Argemone mexicana is biologically active and may have antifungal principles that could be useful in fungal diseases.KYAMC Journal Vol. 4, No.-2, Jan 2014, Page 402-405

Evaluation of α-amylase Inhibition and Cytotoxic Activities of the Arachis hypogaea and Cinnamomum tamala

2020 ◽  
Vol 16 ◽  
Author(s):  
Deedarul Hyder Sani ◽  
Ali Newaz Munna ◽  
Mohammad Salim ◽  
Md. Jahangir Alam ◽  
Md. Jahangir Alam
Keyword(s):  
Brine Shrimp ◽  
Ethanol Extract ◽  
Acetone Extract ◽  
Cytotoxic Activities ◽  
Inhibition Activity ◽  
Inhibition Assay ◽  
Peanut Seed ◽  
Lc50 Value ◽  
Cinnamomum Tamala ◽  
Ic50 Value

Background: Diabetes mellitus is the most occurring non-communicable disease resulting in a high blood glucose level. There has been an immense interest in the development of alternative medicines for diabetes treatment, specifically screening functional foods for phytochemicals with the capability of delaying or preventing glucose absorption through digestive enzymes (e.g. α-amylase) inhibition. So, the development of α-amylase inhibitors derived from natural food products is an alternative way to prevent diabetes mellitus Objective: In this study, organic solvent extracts of the Arachis hypogaea (Peanut) and Cinnamomum tamala (Indian bay leaf /Tejpata) were used to investigate their potential α-amylase inhibition and cytotoxic activities through α-amylase inhibition assay and brine shrimp lethality bioassay respectively Method: The α-amylase inhibition assay was performed using the 3,5-dinitrosalicylic acid method for different concentrations of plant extracts. The optical density (OD) of the solutions were measured to determine the inhibition activity at 540 nm using a spectrophotometer. The cytotoxicity of the plant extracts was measured using brine shrimp (Artemia salina) lethality bioassay Results: Among the different organic solvent extracts, peanut seed ethanol extract showed the highest α-amylase inhibition activity (67.68±8.67%) at 1.25 μg/mL concentration with an IC50 value of 0.61 μg/mL which is very close to standard α-amylase inhibitor Acarbose (72.34±4.23%) with an IC50 value of 0.32 μg/mL while acetone extract of Indian bay leaf exhibited the lowest inhibition activity (47.75±1.63%) with an IC50 value of 1.42 μg/mL at the same concentration. Besides, the maximum cytotoxic activity was found in acetone extract of peanut shell with an LC50 value of 57.87 μg/mL whereas ethanol extract of peanut seed showed the lowest cytotoxicity with an LC50 value of 413.90 μg/mL Conclusion: The result of the present work clearly indicates the potentiality of peanut seed ethanol extract to be used in the management of hyperglycemia as it significantly inhibits α-amylase activity while showing less cytotoxic activities

scholarly journals Efficacy Assessment of Biosynthesized Copper Oxide Nanoparticles (CuO-NPs) on Stored Grain Insects and Their Impacts on Morphological and Physiological Traits of Wheat (Triticum aestivum L.) Plant

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 233
Author(s):  
Ali A. Badawy ◽  
Nilly A. H. Abdelfattah ◽  
Salem S. Salem ◽  
Mohamed F. Awad ◽  
Amr Fouda
Keyword(s):  
Block Design ◽  
Dry Weight ◽  
Triticum Aestivum L ◽  
Copper Oxide Nanoparticles ◽  
Rhyzopertha Dominica ◽  
Stored Grain ◽  
Stabilizing Agents ◽  
Cuo Nps ◽  
Vis Spectroscopy ◽  
Ft Ir

Herein, CuO-NPs were fabricated by harnessing metabolites of Aspergillus niger strain (G3-1) and characterized using UV–vis spectroscopy, XRD, TEM, SEM-EDX, FT-IR, and XPS. Spherical, crystallographic CuO-NPs were synthesized in sizes ranging from 14.0 to 47.4 nm, as indicated by TEM and XRD. EDX and XPS confirmed the presence of Cu and O with weight percentages of 62.96% and 22.93%, respectively, at varied bending energies. FT-IR spectra identified functional groups of metabolites that could act as reducing, capping, and stabilizing agents to the CuO-NPs. The insecticidal activity of CuO-NPs against wheat grain insects Sitophilus granarius and Rhyzopertha dominica was dose- and time-dependent. The mortality percentages due to NP treatment were 55–94.4% (S. granarius) and 70–90% (R. dominica). A botanical experiment was done in a randomized block design. Low CuO-NP concentration (50 ppm) caused significant increases in growth characteristics (shoot and root length, fresh and dry weight of shoot and root, and leaves number), photosynthetic pigments (total chlorophylls and carotenoids), and antioxidant enzymes of wheat plants. There was no significant change in carbohydrate or protein content. The use of CuO-NPs is a promising tool to control grain insects and enhance wheat growth performance.

scholarly journals Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

2017 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Sari Setianingsih ◽  
Rudi Kartika ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Test Results ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Ms Analysis

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

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